Plerixafor (AMD3100) 8HCl

Catalog No.S3013 Synonyms: JM 3100 8HCl

For research use only.

Plerixafor (AMD3100, JM 3100) 8HCl is the hydrochloride of Plerixafor, a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor can be used as an anti-HIV agent.

Plerixafor (AMD3100) 8HCl Chemical Structure

CAS No. 155148-31-5

Selleck's Plerixafor (AMD3100) 8HCl has been cited by 38 publications

Purity & Quality Control

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Biological Activity

Description Plerixafor (AMD3100, JM 3100) 8HCl is the hydrochloride of Plerixafor, a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor can be used as an anti-HIV agent.
Targets
CXCL12 [1]
(Cell-free assay)
CXCR4 [1]
(Cell-free assay)
5.7 nM 44 nM
In vitro

Plerixafor inhibits CXCL12-mediated chemotaxis with a potency lightly better than its affinity for CXCR4. [1] Plerixafor also antagonizes SDF-1/CXCL12 ligand binding with an IC50 of 651 nM. Plerixafor inhibits SDF-1 mediated GTP-binding, SDF-1 mediated calcium flux and SDF-1 stimulated chemotaxis with IC50 of 27 nM, 572 nM and 51 nM, respectively. Plerixafor does not inhibit calcium flux against cells expressing CXCR3, CCR1, CCR2b, CCR4, CCR5 or CCR7 when stimulated with their cognate ligands, nor does Plerixafor inhibit receptor binding of LTB4. Plerixafor does not, on its own, induce a calcium flux in the CCRF–CEM cells, which express multiple GPCRs including CXCR4, CCR4 and CCR7. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHOK1 cells NVfiPFdDTnWwY4Tpc44h[XO|YYm= NI\LUJRFcXOybHHj[Y1mdnRib3[gX|EzPUmfU1TGNYFteGijIH\yc40hS1iFUkSg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|LDDJR|UxRTBwOEGgcm0> Mn7BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd5MUWxNlgoRjF5N{G1NVI5RC:jPh?=
GHOST CXCR4 cell line NUDnW|F7TnWwY4Tpc44h[XO|YYm= M4TnTGlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBi\2GrboP0JGhKXi1zIFzBTUB{fHKjaX6gbY4hT0iRU2SgR3hEWjRiY3XscEBtcW6nLDDJR|UxRTBwOUWgcm0> MlLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR4OUixPFkoRjF2Nkm4NVg6RC:jPh?=
HEK293 cells NEW1[|RHfW6ldHnvckBie3OjeR?= NF[0[WQzKGSjeYO= NIjHUYpCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBVOjBvcnXzbZN1[W62IFjJWlEhVkx2LUOgbY5n\WO2ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhOiCmYYnzMEBKSzVyPUKuN{BvVQ>? MmX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGzNFUoRjF7NEWxN|A2RC:jPh?=
PBMC cells M4mxcmZ2dmO2aX;uJIF{e2G7 MlvVSYZn\WO2aY\lJINwdmOnboTyZZRqd25ib3[gZ49ueG:3bnSgZYdicW6|dDDITXYuOSB6OT62JJN1emGrbjDpckBRSk2FIHPlcIx{NCCHQ{WwQVMvQCCwTR?= MkL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR4OUixPFkoRjF2Nkm4NVg6RC:jPh?=
human MT4 cells MljlSpVv[3Srb36gZZN{[Xl? MYG0JIRigXN? M{PZOGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNicnXwcIlk[XSrb36gZYZ1\XJiNDDkZZl{KGK7IF3UWEBie3OjeTygSWM2OD12IH7N M330WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMESzOlM5Lz5{MEC0N|Y{QDxxYU6=
human Jurkat cells M4niemZ2dmO2aX;uJIF{e2G7 MU\BcpRi\2:waYP0JIFkfGm4aYT5JIF1KEO[Q2K0JIlvKGi3bXHuJGp2emujdDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFOGRkGtbY5lfWOnZDDj[YxtKG2rZ4LheIlwdixiSVO1NF0zPy52IH7N NWXL[21ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxPFgxPzFpPkG5NVg5ODdzPD;hQi=>
MT-4 cells MY\GeY5kfGmxbjDhd5NigQ>? NXv0N4VKTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gc4Yh[2:vcH;1coQh[WejaX7zeEBJUVZvMTDJTWlDKHO2cnHpckBqdiCPVD20JINmdGy|LDDFR|UxRTZ3IH7N Ml3yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR4OUixPFkoRjF2Nkm4NVg6RC:jPh?=
rat IR983F cells MlT6SpVv[3Srb36gZZN{[Xl? MnezSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZUO[Q1yxNkBnem:vIFPYR3I1KGmwIILheEBKWjl6M1[gZ4VtdHNuIFnDOVA:OTB6IH7N M2TZfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEWzO|Y5Lz5zOUC1N|c3QDxxYU6=
CEM-SS cells MW\GeY5kfGmxbjDhd5NigQ>? MX;F[oZm[3SrdnWgZ49v[2WwdILheIlwdiCxZjDjc41xd3WwZDDh[4FqdnO2IFjJWk0yKEyDSTDzeJJicW5iaX6gR2VONVOVIHPlcIx{NCCHQ{WwQVEzPyCwTR?= NHvHUZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNE[5PFE5QSd-MUS2PVgyQDl:L3G+
human HL60 cells M3HEWmZ2dmO2aX;uJIF{e2G7 Ml;oSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZVOGRkHhcJBp[SCocn;tJGNZS1J2IHnuJIh2dWGwIFjMOlAh[2WubIOsJGlEPTB;MUWuNkDPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF6OEC3NUc,OTlzOEiwO|E9N2F-
human MOLT4 cells MnnmSpVv[3Srb36gZZN{[Xl? MYixNFAxKG6P NYjFdZN{UW6qaXLpeIlwdiCxZjDNZYIhOTKJNTDibY5lcW6pIITvJGNZS1J2IHX4dJJme3OnZDDpckBpfW2jbjDNU2xVPCClZXzsd{BifCBzMECwJI5OKGK7IF\BR3Mh[W6jbInzbZM> NGrQ[XM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
human MT2 cells MX7GeY5kfGmxbjDhd5NigQ>? NXO3c4NtOSC3Zz;tUC=> Mnv2OEBl[Xm| MmjHRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDpcoZm[3SnZDDpckBpfW2jbjDNWFIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCCyMkSgZY51cWenbjDwdo9lfWO2aX;uJIF1KDFidXevcWwh[W[2ZYKgOEBl[Xm|IHL5JGVNUVOD M3;5N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[4N|M3Lz5{MUG2PFM{PjxxYU6=
human U87 cells MlfOSpVv[3Srb36gZZN{[Xl? MmrQNVAxOCCwTR?= M4r4RmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiQ2jDVlQhcW5iaIXtZY4hXTh5IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhW0SIMT3pcoR2[2WmIH3v[JVt[XSrb36gc4Yh[0GPUDDwdo9lfWO2aX;uJIF1KDFyMECgcm0h[nliVGKtSnJGXCCjc4PhfS=> NHzqVlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m1PFM1PCd-MUe5OVg{PDR:L3G+
MT4 NHLyRWtCdnSrdnnyZYwh[XO|YYm= NHfMRnJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDjfZRweGG2aHnjJIVn\mWldDDpckBOXDRiY3XscJMtKEWFNUC9NE4xOTIQvF2= NU\UcId7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewN|QyOjJpPkG3NFM1OTJ{PD;hQi=>
U87 NEXCWo5HfW6ldHnvckBie3OjeR?= NGjseGwyPSCvaX7z M37RSWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSg[ZhxemW|c3XkJIlvKGi3bXHuJHU5PyClZXzsd{BmgHC{ZYPzbY5oKEOGNDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPYR2wyOi2rbnT1Z4VlKGODTWCgdJJw\HWldHnvckBxemW2cnXheIVlKG[xcjCxOUBucW6|IHLl[o9z\SCob4Lzb49tcW5iY3jhcIxmdmenIHL5JHRTNU[URWSgZY5idHm|aYOsJGlEPTB;MD62PVXPxE1? M3vmPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUC1O|E2Lz5{MUGwOVcyPTxxYU6=
MT4 MW\BcpRqfmm{YXygZZN{[Xl? MVTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDYOE11em:yaXOgTGlXOSCQTESuN{Bqdm[nY4Tl[EBqdiCqdX3hckBOXDRiY3XscJMh[XO|ZYPz[YQh[XNicILveIVkfGmxbjDmdo9uKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjv[4VvcWOrdImsJGVEPTB;MD6wOlLPxE1? M{fQdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUe5OFE5Lz5{MkW3PVQyQDxxYU6=
MT4 NF\ZdIZCdnSrdnnyZYwh[XO|YYm= NV3i[VVTPSCmYYnz M1fPTmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiaX7m[YN1\WRiaX6gUXQ1KGOnbHzzJIV5eHKnc4PpcochS1iFUkSgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGGodHXyJFUh\GG7czygSWM2OD1yLkCwNu69VQ>? NYXiRXJNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NFkxQDhpPkKyPVA6ODh6PD;hQi=>
CHO NV7jPWt{TnWwY4Tpc44h[XO|YYm= M4r5ZmJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6gdoVkd22kaX7hcpQhS1iFUkSg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3K9NE4xPzgQvF2= NEHGPWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwPVA5QCd-MkK5NFkxQDh:L3G+
CHO NX:0VHBmTnWwY4Tpc44h[XO|YYm= NWDCd49bSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiC{ZXPvcYJqdmGwdDDDXGNTPCCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV2RHOWFvaX7keYNm\CCnbHXjeJJq[2GuIHntdIVl[W6lZTDifUBlcWWuZXP0dolkKHOyZXP0do9{[2:yaXOgZY5idHm|aYOsJGlEPTB;MD6yOu69VQ>? NGHSVJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwPVA5QCd-MkK5NFkxQDh:L3G+
CD44null NGTqW2hHfW6ldHnvckBie3OjeR?= NHPNVWM4KGSjeYO= NIrUNmtG\m[nY4Sgc44hcHWvYX6gS2JOOiClZXzsJIRq\m[ncnXueIlifGmxbjDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCFRES0cpVtdCClZXzsd{Bi\nSncjC3JIRigXNiYomg[oxwfyCleYTvcYV1emmlIHHuZYx6e2m| Mo\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MEmwPFgoRjJ{OUC5NFg5RC:jPh?=
CD44null MYXGeY5kfGmxbjDhd5NigQ>? MV[3JIRigXN? NYnmNVFbTW[oZXP0JI9vKGi3bXHuJGdDVTFiY3XscEBlcW[oZYLlcpRq[XSrb36gZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iQ1S0OI52dGxiY3XscJMh[W[2ZYKgO{Bl[Xm|IHL5JIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MmLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MEmwPFgoRjJ{OUC5NFg5RC:jPh?=
Assay
Methods Test Index PMID
Immunofluorescence CXCR4 ; β-arrestin2 28521261 31186083
In vivo A single topical application of Plerixafor promotes wound healing in diabetic mice by increasing cytokine production, mobilizing bone marrow EPCs, and enhancing the activity of fibroblasts and monocytes/macrophages, thereby increasing both angiogenesis and vasculogenesis. [3] Cohorts of mice are administered with PBS, IGF1, PDGF, SCF, or VEGF for five consecutive days and Plerixafor on the 5th day. The number and size of the colonies are highest in IGF1 plus Plerixafor injected mice compared to PDGF, SCF and VEGF treated groups, in combination with Plerixafor. [4]

Protocol (from reference)

Animal Research:[4]
  • Animal Models: Twelve-week-old C57BL/6 mice with segmental bone defect
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 794.47
Formula

C28H54N8.8HCl

CAS No. 155148-31-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CNCCNCCCN(CCNC1)CC2=CC=C(C=C2)CN3CCCNCCNCCCNCC3.Cl.Cl.Cl.Cl.Cl.Cl.Cl.Cl

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04646603 Active not recruiting Drug: MRG-001|Drug: Placebo COVID-19 MedRegen LLC|ICON plc January 28 2021 Phase 1|Phase 2
NCT03547830 Recruiting Drug: Plerixafor|Drug: Gcsf Chronic Granulomatous Disease Federal Research Institute of Pediatric Hematology Oncology and Immunology April 13 2019 Phase 2
NCT03653247 Recruiting Biological: Plerixafor|Drug: Busulfan|Genetic: BIVV003 Sickle Cell Disease Bioverativ a Sanofi company|Sanofi March 6 2019 Phase 1|Phase 2
NCT03442673 Recruiting Drug: Vinorelbine|Drug: Gemcitabine|Drug: G-CSF Multiple Myeloma University Hospital Inselspital Berne September 17 2018 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

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