Piperlongumine

For research use only.

Catalog No.S7551 Synonyms: PPLGM, Piplartine

18 publications

Piperlongumine Chemical Structure

CAS No. 20069-09-4

Piperlongumine (PPLGM, Piplartine), a natural alkaloid from Piper longum L., increases the level of reactive oxygen species (ROS) and selectively kills cancer cells. It is a direct TrxR1 inhibitor with suppressive activity against gastric cancer and a novel inhibitor of CRM1; also an inhibitor of PI3K/Akt/mTOR in human breast cancer cells.

Selleck's Piperlongumine has been cited by 18 publications

2 Customer Reviews

  • a. Cell growth of ARID1A-wildtype RMG1 cells transfected with ARID1A and non-target siRNA for 24 h and treated with piperlongumine for 72 h. b. Apoptosis of RMG1 cells after transfection and treatment as described in a as measured using annexin-V and PI staining. c. Cell growth of RMG1 cells transfected and treated with 5 μM of piperlongumine as described in a, but in the presence or absence of the antioxidant NAC. Cell growth was measured using the WST-1 assay and quantified relative to DMSO treated non-target control. *P < 0.05; **P < 0.01; ***P < 0.001.

    Oncotarget, 2016, 7(35):56933-56943. Piperlongumine purchased from Selleck.

  • MPC cells were treated with the indicated concentrations of PL at 21% and 1% O2 for 24 hours. Total cell lysates were subjected to Western blot with antibodies against cleaved PARP, cleaved caspase 3, and caspase 3. β-tubulin was used as a loading control. A representative image (n=3) is shown.

    Oncotarget, 2016, 7(26):40531-40545. Piperlongumine purchased from Selleck.

Purity & Quality Control

Choose Selective ROS Inhibitors

Biological Activity

Description Piperlongumine (PPLGM, Piplartine), a natural alkaloid from Piper longum L., increases the level of reactive oxygen species (ROS) and selectively kills cancer cells. It is a direct TrxR1 inhibitor with suppressive activity against gastric cancer and a novel inhibitor of CRM1; also an inhibitor of PI3K/Akt/mTOR in human breast cancer cells.
Targets
reactive oxygen species (ROS) [1] TrxR1 [5] CRM1 [6] PI3K/Akt/mTOR [7]
In vitro

Piperlongumine is a known ROS inducer which could induce pancreatic cancer cell death in cell culture[1] As a thromboxane A(2) receptor antagonist, Piperlongumine inhibits platelet aggregation. [2] Piperlongumine also promotes autophagy via inhibition of Akt/mTOR signalling and mediates cancer cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ZR75-30 M3zZN2N6fG:2b4jpZ4l1gSCjc4PhfS=> M3zyfFczKGi{cx?= NWr5c4x1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hYlJ5NT2zNEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTVwOEdOwG0> NVzVbVhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5N|cyQDZpPkK0PVM4OTh4PD;hQi=>
HCT116 NU\oeW1qS3m2b4TvfIlkcXS7IHHzd4F6 M4P3R|czKGi{cx?= NVHwT5pGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Pi5yNN88US=> M3\YNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUO3NVg3Lz5{NEmzO|E5PjxxYU6=
MDA-MB-231 MlnJR5l1d3SxeHnjbZR6KGG|c3H5 NIe5T3Q4OiCqcoO= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QC52Nt88US=> MnzLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7M{exPFYoRjJ2OUO3NVg3RC:jPh?=
A549 NV;sTINrS3m2b4TvfIlkcXS7IHHzd4F6 Mn7UO|IhcHK| M4XkemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yNk45Pc7:TR?= NE\He|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmzO|E5Pid-MkS5N|cyQDZ:L3G+
WI38 M4\pNmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2i1OFczKGi{cx?= MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDXTVM5KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mk[uO|jPxE1? M3v5elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUO3NVg3Lz5{NEmzO|E5PjxxYU6=
MRC5 NVq1e2xFS3m2b4TvfIlkcXS7IHHzd4F6 NFW2eo84OiCqcoO= NHzoZ|JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|UvODUQvF2= M1rLNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUO3NVg3Lz5{NEmzO|E5PjxxYU6=
PANC1 MX7DfZRwfG:6aXPpeJkh[XO|YYm= M1nFW|I1KGi{cx?= M1LmWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBCVkNzIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliTWTUJJJm\HWldHnvckBie3OjeTygTWM2OD1|LkNOwG0> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNUexPEc,OjV|MEW3NVg9N2F-
HaCaT NXq4TIR5S3m2b4TvfIlkcXS7IHHzd4F6 MlPONlQhcHK| MoOzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIFE[VRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGSgdoVlfWO2aX;uJIF{e2G7LDDJR|UxRTF4zszN MlXFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEW3NVgoRjJ3M{C1O|E5RC:jPh?=
RAW264.7 NWDRUIRtSW62aXnu[oxidW2jdH;yfUBie3OjeR?= NYLVU3drSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25uIFnDOVA:O87:TR?= Mny0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NUO4NFkoRjJ3NEWzPFA6RC:jPh?=
MA9.3 MkLxR5l1d3SxeHnjbZR6KGG|c3H5 NGCyWG41QCCqcoO= NYrybI93S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUF7LkOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0z|ryP NFH3fGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2OFg5Pyd-MkW0OlQ5QDd:L3G+
SK-MEL-2 NV;FW2IzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M3[0TlczKGi{cx?= NGDwSJpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW1GVC1{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEWFNUC9OE4{Qc7:TR?= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NkO5PEc,OjV6Mk[zPVg9N2F-
HaCaT M1m0bGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGXDd3U4OiCqcoO= NEH6UmdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjhR4FVKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;ND60Ou69VQ>? M1Gwd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK2N|k5Lz5{NUiyOlM6QDxxYU6=
A375 M1zycGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 Mlr1O|IhcHK| NVPpR|d5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;Nj6xO:69VQ>? NEXUWlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOlM6QCd-MkW4NlY{QTh:L3G+
HCT116 M{[4bGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWnXOlVuPzJiaILz MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTFzLkZOwG0> NYLFSmI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlY{QThpPkK1PFI3Ozl6PD;hQi=>
A549 MYrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFm4Ook4OiCqcoO= M1uzUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTF{LkNOwG0> NVjGdYpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NlY{QThpPkK1PFI3Ozl6PD;hQi=>
HFF1 MmPTRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmDtO|IhcHK| Mo[yRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDISmYyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;MUOuNe69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh{NkO5PEc,OjV6Mk[zPVg9N2F-
MCF10A NEnhV4FCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MlXFO|IhcHK| M3HzVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGNVBCKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;MUiuNe69VQ>? NGC3NZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOlM6QCd-MkW4NlY{QTh:L3G+
A549 MonrSpVv[3Srb36gZZN{[Xl? Mo\FN{BpenN? MoH0TY5lfWO2aX;uJI9nKFKRUzDwdo9lfWO2aX;uJIlvKGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgN{BpenNiYomgSGhGKHO2YXnubY5oNWKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfS=> M{O0OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK2N|k5Lz5{NUiyOlM6QDxxYU6=
A549 Ml;5SpVv[3Srb36gZZN{[Xl? Ml\tN{BpenN? MnHLTY5lfWO2aX;uJI9nKFKRUzDwdo9lfWO2aX;uJIlvKGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgN{BpenNiYomgRXBHKHO2YXnubY5oNWKjc3XkJIZtfW:{ZYPj[Y5k\SCjc4PhfS=> MkT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6Mk[zPVgoRjJ3OEK2N|k5RC:jPh?=
A549 MVXGeY5kfGmxbjDhd5NigQ>? M1uySVExKHWP NELEOGI1KGi{cx?= NUixPYhvWmWmdXP0bY9vKGmwIITveIFtKEeVSDDs[ZZmdCCrbjDoeY1idiCDNUS5JINmdGy|IHH0JFExKHWPIHHmeIVzKDRiaILzJIJ6KGy3bXnu[ZNk\W6lZTDhd5NigQ>? NH\aU2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUiyOlM6QCd-MkW4NlY{QTh:L3G+
A549 NGrKSZpHfW6ldHnvckBie3OjeR?= M2DMbFExKHWP Ml:2OEBpenN? M2Xy[XJm\HWldHnvckBqdiC{ZXT1Z4VlKEeVSDDs[ZZmdCCrbjDoeY1idiCDNUS5JINmdGy|IHH0JFExKHWPIHHmeIVzKDRiaILzJIJ6KGy3bXnu[ZNk\W6lZTDhd5NigQ>? M174WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK2N|k5Lz5{NUiyOlM6QDxxYU6=
HT1080 MlnaR5l1d3SxeHnjbZR6KGG|c3H5 MkH5O|IhcHK| MVjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWFExQDBiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgO|IhcHK|IHL5JJJme2G8dYLpckBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;Mz60N:69VQ>? NHTjTFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5PVU{OCd-Mk[1PVk2OzB:L3G+
GBM10 Mo[wR5l1d3SxeHnjbZR6KGG|c3H5 Mne4O|IhcHK| M3TISmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGdDVTFyIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBz\XOjeoXybY4hemWmdXP0bY9vKGG|c3H5MEBKSzVyPUOuPFHPxE1? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7OUWzNEc,OjZ3OUm1N|A9N2F-
SF188 MWXDfZRwfG:6aXPpeJkh[XO|YYm= NY\SOoszPzJiaILz NHzJNIZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUTjF6ODDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhemW|YYr1dolvKHKnZIXjeIlwdiCjc4PhfUwhUUN3ME2zMlnPxE1? M2\s[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm5OVMxLz5{NkW5PVU{ODxxYU6=
SKBR3 MUjDfZRwfG:6aXPpeJkh[XO|YYm= NWW4UWM5PzJiaILz MkmxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tDWjNiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgO|IhcHK|IHL5JJJme2G8dYLpckBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;ND6wOO69VQ>? M4LZNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUm5OVMxLz5{NkW5PVU{ODxxYU6=
T98G NYn1TZg2S3m2b4TvfIlkcXS7IHHzd4F6 NVLEOYtLPzJiaILz M2X0eGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHQ6QEdiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgO|IhcHK|IHL5JJJme2G8dYLpckBz\WS3Y4Tpc44h[XO|YYmsJGlEPTB;ND65Nu69VQ>? NXu0dohQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVk2OzBpPkK2OVk6PTNyPD;hQi=>
HT-29 MlX4R5l1d3SxeHnjbZR6KGG|c3H5 M4PYfVczKGi{cx?= MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC3NkBpenNiYomgdoV{[Xq3cnnuJJJm\HWldHnvckBie3OjeTygTWM2OD13LkS5{txO NUTCRZQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVk2OzBpPkK2OVk6PTNyPD;hQi=>
MCF7 NHnle3BEgXSxdH;4bYNqfHliYYPzZZk> MWK3NkBpenN? NUjHZ5Z7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhemW|YYr1dolvKHKnZIXjeIlwdiCjc4PhfUwhUUN3ME22MlE1|ryP NGPD[5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5PVU{OCd-Mk[1PVk2OzB:L3G+
A549 M1L4fGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXW3NkBpenN? NIftd3REgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBz\XOjeoXybY4hemWmdXP0bY9vKGG|c3H5MEBKSzVyPU[uOVTPxE1? NFTweoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5PVU{OCd-Mk[1PVk2OzB:L3G+
MV4-11 NEfPVHhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NUTEWWxyPzJiaILz M1T2dGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTW[0MVEyKGOnbHzzJIFnfGW{IEeyJIhzeyCrbjDwdoV{\W6lZTDv[kBUSUiDIHL5JG1VXCCjc4PhfUwhUUN3ME2wMlI5QTIQvF2= MnXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
CMK NHnq[FhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXntVFlHPzJiaILz MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFHyZU1EKHKnc3nzeIFvfCCqdX3hckBEVUtiY3XscJMh[W[2ZYKgO|IhcHK|IHnuJJBz\XOnbnPlJI9nKFODSFGgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvPDJzON88US=> MmjsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
CMY MnnzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mn;IO|IhcHK| M3jNXmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ13ZJINmdGy|IHHmeIVzKDd{IHjyd{BqdiCycnXz[Y5k\SCxZjDTRWhCKGK7IF3UWEBie3OjeTygTWM2OD1yLkS0NlTPxE1? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
HL60 NUW2fotWSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHvxPHc4OiCqcoO= MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIlvKHC{ZYPlcoNmKG:oIGPBTGEh[nliTWTUJIF{e2G7LDDJR|UxRTBwNUK5Nu69VQ>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
CMK MnTnRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGjvVnU4OiCqcoO= NWTNNpVySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDUWsh[2WubIOgZYZ1\XJiN{KgbJJ{KGmwIIDy[ZNmdmOnIH;mJHNCUEFiYomgUXRVKGG|c3H5MEBKSzVyPUCuOlEzP87:TR?= NF;mRWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WwOVg1QCd-Mke1NFU5PDh:L3G+
U937 MoX3RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MVW3NkBpenN? Mk\lRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDwOVMhdnWubDDoeY1idiCXOUO3JINmdGy|IHHmeIVzKDd{IHjyd{BqdiCycnXz[Y5k\SCxZjDTRWhCKGK7IF3UWEBie3OjeTygTWM2OD1yLke2O:69VQ>? M3nMelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUC1PFQ5Lz5{N{WwOVg1QDxxYU6=
MOLM13 NV3Od|c3SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NInWd5c4OiCqcoO= MnHVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPT1zNNVMh[2WubIOgbIFz[m:{aX7nJJdqdGRidInw[UBxPTNxRlzUN{1KXERibYX0ZY51KGGodHXyJFczKGi{czDpckBxemW|ZX7j[UBw\iCVQVjBJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjl|NEZOwG0> MlPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
CMS NV;iRWRySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1rRb|czKGi{cx?= M2LlTmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ13TJINmdGy|IHHmeIVzKDd{IHjyd{BqdiCycnXz[Y5k\SCxZjDTRWhCKGK7IF3UWEBie3OjeTygTWM2OD1zLkCzO:69VQ>? NHrzR5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WwOVg1QCd-Mke1NFU5PDh:L3G+
OCI-AML3 NWC1[5p6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NXG0NpVWPzJiaILz Mn3VRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCRQ1mtRW1NOyClZXzsd{Bi\nSncjC3NkBpenNiaX6gdJJme2WwY3Wgc4YhW0GKQTDifUBOXFRiYYPzZZktKEmFNUC9NU4yODoQvF2= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
HL60 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFfISow4OiCqcoO= M1fNSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgRZJiNUNicnXzbZN1[W62IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIlvKHC{ZYPlcoNmKG:oIGPBTGEh[nliTWTUJIF{e2G7LDDJR|UxRTFwMUK4{txO M1TxNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUC1PFQ5Lz5{N{WwOVg1QDxxYU6=
MV4-11 NVLEZoJSSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MWm3NkBpenN? NEm2XYRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3WOE0yOSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOFM6|ryP MojWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
CMY NHPlO3ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M2G5TFczKGi{cx?= M3XFZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ13ZJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS54Mt88US=> NUW4[HRCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NFU5PDhpPkK3OVA2QDR6PD;hQi=>
HL60 M4rGNmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M4rXPFczKGi{cx?= NXT0VYtuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT63O|jPxE1? M2jXVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUC1PFQ5Lz5{N{WwOVg1QDxxYU6=
MOLM13 NGfSZ|ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MkLjO|IhcHK| M3zrfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE{KGOnbHzzJIhiemKxcnnu[{B4cWymIIT5dIUheDV|L1\MWFMuUVSGIH31eIFvfCCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvQDN6zszN NULWSINtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NFU5PDhpPkK3OVA2QDR6PD;hQi=>
OCI-AML3 NIfiWHhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M17qV|czKGi{cx?= NV7TPIdOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDPR2kuSU2OMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvQDZ6zszN MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
CMK NEf3fYhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NInOZ4w4OiCqcoO= MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFHyZU1EKHKnc3nzeIFvfCCqdX3hckBEVUtiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlkyOc7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
U937 M3vVemFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGfNS|Q4OiCqcoO= MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IIC1N{BvfWyuIHj1cYFvKFV7M{egZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zNjF5NN88US=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
HL60 MV3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGmzRmk4OiCqcoO= NFjaOVJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGFz[S2FIILld4l{fGGwdDDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Oi5{Nd88US=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
CMK MmLWRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NFTsbo04OiCqcoO= MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEOPSzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQDF3zszN Mn:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
CMS NITZb5lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4rYblczKGi{cx?= M1[xOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ13TJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Oy5yOUhOwG0> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzVyNUi0PEc,Ojd3MEW4OFg9N2F-
U937 NYrUTG84SXCxcITvd4l{KGG|c3H5 MlPoNkB2VQ>? MUiyOEBpenN? NWnyZmk5UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBxPTNiboXscEBpfW2jbjDVPVM4KGOnbHzzJIF{e2W|c3XkJIF{KGOjc4Dhd4UhOyClbHXheoFo\SCjdDCyJJVOKGGodHXyJFI1KGi{czDpckBxemW|ZX7j[UBw\iCVQVjBJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= M{jBTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUC1PFQ5Lz5{N{WwOVg1QDxxYU6=
U937 Mn;6RZBweHSxc3nzJIF{e2G7 Ml3lNkB2VQ>? Mn\INlQhcHK| NIPI[GlKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJJA2OyCwdXzsJIh2dWGwIGW5N|ch[2WubIOgZZN{\XO|ZXSgZZMhWEGUUD2xJINt\WG4YXflJIF1KDJidV2gZYZ1\XJiMkSgbJJ{KGmwIIDy[ZNmdmOnIH;mJHNCUEFiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ NIHQZ4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WwOVg1QCd-Mke1NFU5PDh:L3G+
U937 NXjBO|BSS2WubDDk[YF1cCCjc4PhfS=> MYexJJRwKDRidV2= MUmyOEBpenN? MlfVTY5lfWO2aX;uJI9nKGOnbHyg[IVifGhiaX6gdFU{KG63bHygbJVu[W5iVUmzO{Bk\WyuczDheEAyKHSxIESgeW0h[W[2ZYKgNlQhcHK|IHL5JIFvdmW6aX6gWk1HUVSFL2DJJJN1[WmwaX7nJIJie2WmIH\sc5ch[3m2b33leJJ6 M1L6fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUC1PFQ5Lz5{N{WwOVg1QDxxYU6=
U937 NU\Bb|BmS3m2b4TvfIlkcXS7IHHzd4F6 NGH4cnUxNjVidH:gOUB2VQ>? MXqyOEBpenN? MmWyR5l1d3SxeHnjbZR6KGGpYXnud5QheDV|IH71cIwhcHWvYX6gWVk{PyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDj[YxtKH[rYXLpcIl1gSCjdDCwMlUhfG9iNTD1UUBi\nSncjCyOEBpenNiaX6gdJJme2WwY3Wgc4YhW0GKQTDifUBOXFRiYYPzZZk> NHXOWoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WwOVg1QCd-Mke1NFU5PDh:L3G+
U937 M4nQRWZ2dmO2aX;uJIF{e2G7 NX7CZWVVOiC3TR?= Ml7INlAhcHK| MX3JcoR2[3Srb36gc4YhTE6DIHThcYFo\SCrbjDwOVMhdnWubDDoeY1idiCXOUO3JINmdGy|IHH0JFIhfU1iYX\0[ZIhOjBiaILzJIlvKHC{ZYPlcoNmKG:oIGPBTGEh[nliU2nCVkBod2ymIIP0ZYlvcW6pIHLhd4VlKGGua3HsbY5mKGOxbXX0JIF{e2G7 MkHXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3MEW4OFgoRjJ5NUC1PFQ5RC:jPh?=
MCF7 M3rz[2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXO0PEBpenN? NH2yWlVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBidGGvYYLCcJVmKGG|c3H5MEBKSzVyPUGuNu69VQ>? MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OEm3NlgoRjJ5Nki5O|I5RC:jPh?=
Jurkat NVyybWw6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MXG0PEBpenN? NITafY5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBqdiCqdX3hckBLfXKtYYSgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KGGuYX3hdmJtfWViYYPzZZktKEmFNUC9NU41|ryP NUe1XGRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PFk4OjhpPkK3Olg6PzJ6PD;hQi=>
Jurkat NXPRPFdiS3m2b4TvfIlkcXS7IHHzd4F6 M4nScFI1KGi{cx?= NGDyNHhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBLfXKtYYSgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF02|ryP NF24PZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PVczQCd-Mke2PFk4Ojh:L3G+
MCF7 NHnWV4JEgXSxdH;4bYNqfHliYYPzZZk> NYf4NZVQOjRiaILz NXfaeoxES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVjPxE1? M1LXSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Nki5O|I5Lz5{N{[4PVczQDxxYU6=
MCF7 MYTBdI9xfG:|aYOgZZN{[Xl? M374UVExKHWP NVTTd3BDPDhiaILz MWXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[I94dnKnZ4XsZZRqd25ib3[gRoNtOiCneIDy[ZN{cW:wIHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m NITH[Y49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PVczQCd-Mke2PFk4Ojh:L3G+
MCF7 NED3VGxCeG:ydH;zbZMh[XO|YYm= M4fnelExKHWP NFXjWnM4OiCqcoO= Mn3vTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJG1kdC1zIHX4dJJme3Orb36gZZQhOTBidV2gZYZ1\XJiN{KgbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S= NF\4bXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PVczQCd-Mke2PFk4Ojh:L3G+
MCF7 MlnJSpVv[3Srb36gZZN{[Xl? NW\GcY5pOjBidV2= NYLWUGRuOTZiaILz NYW3eXR[TGm|coXweIlwdiCxZjDtbYNzd3S3YoXs[UBv\XS5b4LrJIlvKGi3bXHuJG1ETjdiY3XscJMh[XRiMkCgeW0h[W[2ZYKgNVYhcHK|IHL5JIludXWwb3\seY9z\XOlZX7j[UBidmGueYPpdy=> MlLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OEm3NlgoRjJ5Nki5O|I5RC:jPh?=
MCF7 M{TxUWZ2dmO2aX;uJIF{e2G7 MonWNVAhfU1? NY\PN3BKOTZiaILz NImxR3lKdmS3Y4Tpc44hd2ZibXnjdo91fWK3bHWg[IVxd2y7bXXybZpifGmxbjDpckBpfW2jbjDNR2Y4KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEG2JIhzeyCkeTDpcY12dm:obIXvdoV{[2WwY3WgZY5idHm|aYO= NVzSblZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PFk4OjhpPkK3Olg6PzJ6PD;hQi=>
MCF7 NGLTeIpHfW6ldHnvckBie3OjeR?= M3H1OlEhfG9iMkCgeW0> Mn7vOFghcHK| NW[weVdoUW6mdXP0bY9vKG:oIILlZYN1cX[nIH;4fYdmdiC|cHXjbYV{KGenbnXyZZRqd25iaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBifCBzIITvJFIxKHWPIHnuJJBz\XOnbnPlJI9nKHS{b3zvfEBi\nSncjC0PEBpenNiYomgZYxidWG{Qnz1[UBie3OjeR?= M4T3eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Nki5O|I5Lz5{N{[4PVczQDxxYU6=
MCF7 MWHGeY5kfGmxbjDhd5NigQ>? MnPTOUB1dyB{MDD1US=> NInRb2Y1KGi{cx?= M3Wxdmlv\HWldHnvckBw\iC2dXL1cIlvKGSncH;sfY1memm8YYTpc44hcW5iaIXtZY4hVUOINzDj[YxteyCjdDC1JJRwKDJyIIXNJIFnfGW{IESgbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S= MoL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OEm3NlgoRjJ5Nki5O|I5RC:jPh?=
Jurkat NInPSWFHfW6ldHnvckBie3OjeR?= M2LTflUhfG9iMkCgeW0> MoK1OEBpenN? NVfTVYI3UW6mdXP0bY9vKG:oIIT1ZpVtcW5iZHXwc4x6dWW{aYrheIlwdiCrbjDoeY1idiCMdYLrZZQh[2WubIOgZZQhPSC2bzCyNEB2VSCjZoTldkA1KGi{czDifUBY\XO2ZYLuJIJtd3RibXX0bI9l M1S5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Nki5O|I5Lz5{N{[4PVczQDxxYU6=
A549 MkjOSpVv[3Srb36gZZN{[Xl? NIKxPIUzPCCqcoO= NEHuWm1KdmirYnn0bY9vKG:oIF7GMYtieHCjQjDpckBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NU44Ps7:TR?= NIDKfY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ixNFU6PCd-Mke4NVA2QTR:L3G+
HepG2 MkGyR5l1d3SxeHnjbZR6KGG|c3H5 MYi3NkBpenN? MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVgvQc7:TR?= MmKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzNUm0NVUoRjJ6MUW5OFE2RC:jPh?=
HCT116 M1vES2N6fG:2b4jpZ4l1gSCjc4PhfS=> M17BelczKGi{cx?= M1zBUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTlwMt88US=> NVmyb5JyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixOVk1OTVpPkK4NVU6PDF3PD;hQi=>
A549 MVjDfZRwfG:6aXPpeJkh[XO|YYm= MYC3NkBpenN? M4PWOmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOM7:TR?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF3OUSxOUc,OjhzNUm0NVU9N2F-
MRC5 MlnIR5l1d3SxeHnjbZR6KGG|c3H5 NXvseXBjPzJiaILz NFvPfnJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9N|fPxE1? NICzZXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG1PVQyPSd-MkixOVk1OTV:L3G+
A549 NV3hcI1CSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVK3NkBpenN? M1XUc2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTZwOEVOwG0> M3rVVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEO0O|Y1Lz5{OESzOFc3PDxxYU6=
Saos2 NF7QOotCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFGxd204OiCqcoO= MmnYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVYX;zNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTdwM{JOwG0> Mm\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{S3OlQoRjJ6NEO0O|Y1RC:jPh?=
HCT116 MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MUC3NkBpenN? NX3aTo5kSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME23MlM1|ryP M{\JblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEO0O|Y1Lz5{OESzOFc3PDxxYU6=
U2OS NFnpRYRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXPMS|lNPzJiaILz Mk\BRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXMl;TJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QS52Od88US=> Mmr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{S3OlQoRjJ6NEO0O|Y1RC:jPh?=
MDA-MB-231 M1H2OWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NV64OmR1PzJiaILz M1TZVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVExNjcQvF2= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR|NEe2OEc,Ojh2M{S3OlQ9N2F-
SKHEP1 NUTQOnZVSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MWm3NkBpenN? Ml\nRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVS1jFVFEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMz6z{txO NIHSRXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESzOFc3PCd-Mki0N|Q4PjR:L3G+
HCT116 MlPHSpVv[3Srb36gZZN{[Xl? MYKxNEB2VQ>? MVKxJIhz Mn;QTY5lfWO2aX;uJI9nKHKnYXP0bZZmKG:6eXflckB{eGWlaXXzJIdmdmW{YYTpc44hcW5iaIXtZY4hUEOWMUG2JINmdGy|IHH0JFExKHWPIHHmeIVzKDFiaIKgZpkhTEOISD3ERUBlgWVvYnHz[YQh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nz MlOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2M{S3OlQoRjJ6NEO0O|Y1RC:jPh?=
HCT116 NFm1R2xE\WyuIHP5Z4xmKGG|c3H5 MW[xJJRwKDVidV2= MoroNlQhcHK| NY\oOVJ2S2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIFk[3WvdXzheIlwdiCjdDDTJJBp[XOnIEGgeI8hPSC3TTDh[pRmeiB{NDDodpMh[nlicILvdIllcXWvIHnv[Ill\SC|dHHpcolv\yCkYYPl[EBndG:5IHP5eI9u\XS{aXOgcYV1cG:m NIXDOFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESzOFc3PCd-Mki0N|Q4PjR:L3G+
HCT116 NH\xVWtE\WyuIHP5Z4xmKGG|c3H5 MVOxJJRwKDVidV2= NF;IOmkzPCCqcoO= Mn\tR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMD;HNUBxcGG|ZTDheEAyKHSxIEWgeW0h[W[2ZYKgNlQhcHK|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnegZoF{\WRiZnzve{BkgXSxbXX0dolkKG2ndHjv[C=> M33YelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEO0O|Y1Lz5{OESzOFc3PDxxYU6=
HCT116 NGq0NFdE\WyuIHP5Z4xmKGG|c3H5 MXGxJJRwKDVidV2= MnjvNlQhcHK| M{TTemNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGhEXDFzNjDj[YxteyCmZXPy[YF{\SCrbjDwc5B2dGG2aX;uJIF1KEdyL1exJJBp[XOnIHH0JFEhfG9iNTD1UUBi\nSncjCyOEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTybYMhdWW2aH;k M2XheVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEO0O|Y1Lz5{OESzOFc3PDxxYU6=
HT-29 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYOzbWR2PzJiaILz NGH5OGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mj62Oe69VQ>? NVjzZlM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFY6OTFpPkK4Olg3QTFzPD;hQi=>
HCT8 M4[wZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGTiOpU4OiCqcoO= M2TNOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUPEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTRwMd88US=> NYHaVmFJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFY6OTFpPkK4Olg3QTFzPD;hQi=>
HCT116 NYnoclYxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFrueW44OiCqcoO= NWDieFF2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME24MlE{|ryP NIDqdmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4OlkyOSd-Mki2PFY6OTF:L3G+
CCD-841 MnLER5l1d3SxeHnjbZR6KGG|c3H5 NY\QeFVXPzJiaILz MnXLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2NFNTh2MTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD12ND6zNu69VQ>? NH3TRYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4OlkyOSd-Mki2PFY6OTF:L3G+
HCT116 MnHwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlfENVAhfU1? Mmq0O|IhcHK| NFWyWmFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{BifCBzMDD1UUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5 M1;NfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki2PVEyLz5{OE[4OlkyOTxxYU6=
A549 M4rkPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHHEUJEyOCC3TR?= Mn[5O|IhcHK| MkT0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHH0JFExKHWPIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[Xl? NVvDSGU1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFY6OTFpPkK4Olg3QTFzPD;hQi=>
K562 M3TWVWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHnmWI0yOCC3TR?= Ml3RO|IhcHK| NGmwSmlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubIOgZZQhOTBidV2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?= M3TrSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki2PVEyLz5{OE[4OlkyOTxxYU6=
HT-29 MlK4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NILzSYk4OiCqcoO= MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Oi54Nd88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
HCT8 NF7nd5VCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NWXHZppSPzJiaILz MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEigZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01NjIQvF2= Ml3OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
SW620 NWDRTI44SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MlvtO|IhcHK| NITwbVZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXOlIxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;ND62Nu69VQ>? NFTZb4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSyOFU{QSd-Mkm0NlQ2Ozl:L3G+
K562 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYPRd2NEPzJiaILz NVjDSHE1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NT6wOe69VQ>? M3H0OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
K562 Ml7YRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MVm3NkBpenN? NI\DV5ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiN{KgbJJ{KGmwIIDy[ZNmdmOnIH;mJIxq\3W|dILhfolv\SCkeTDNWHQh[XO|YYmsJGlEPTB;NT6wPe69VQ>? Mm\TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
HCT116 NYHNWZNTSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEHvRWc4OiCqcoO= MnLwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD16LkGz{txO MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
HCT116 NH3ZSXVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mo\wO|IhcHK| MnjORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGmwIIDy[ZNmdmOnIH;mJIxq\3W|dILhfolv\SCkeTDNWHQh[XO|YYmsJGlEPTB;OD6xO:69VQ>? NVHub4ltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NlQ2OzlpPkK5OFI1PTN7PD;hQi=>
A549 M3q1dmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnflO|IhcHK| NEj3dYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiN{KgbJJ{KGmwIIDy[ZNmdmOnIH;mJIxq\3W|dILhfolv\SCkeTDNWHQh[XO|YYmsJGlEPTB;MUWuNlLPxE1? Mn;MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
A549 NW\PcJRySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MlvnO|IhcHK| MnTWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTVwMklOwG0> M1P5bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
U87MG M2\OTmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHrOdIg4OiCqcoO= MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6N13HJINmdGy|IHHmeIVzKDd{IHjyd{BqdiCycnXz[Y5k\SCxZjDsbYd2e3S{YYrpcoUh[nliTWTUJIF{e2G7LDDJR|UxRTF4LkG1{txO NIPCUXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSyOFU{QSd-Mkm0NlQ2Ozl:L3G+
U87MG NYC3T|E4SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MoDJO|IhcHK| NV6zdJF4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFdOTyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG3MlM1|ryP MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
CCD-841 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M33DZ|czKGi{cx?= NYKySYtuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDR2QuQDRzIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFQvOzMQvF2= NGLO[2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSyOFU{QSd-Mkm0NlQ2Ozl:L3G+
HCT116 MXjBcpRqdWmpcnH0c5J6KGG|c3H5 NYqzcZBmPTBibl2= M2fle|Q5KGi{cx?= NX;ydYpOSW62aX3p[5JifG:{eTDhZ5Rqfmm2eTDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBVT0ZvYnX0ZVEucW6mdXPl[EBk\WyuIH3p[5JifGmxbjDheEA2OCCwTTDh[pRmeiB2ODDodpMh[nlidILhcpMuf2WubDDhd5NigQ>? M{n5flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
HCT116 M{TNb2Z2dmO2aX;uJIF{e2G7 MU[xNEB2VQ>? MXyzJIhzew>? MlvjTY5lfWO2aX;uJI9nKFKRUzDhZ4N2dXWuYYTpc44hcW5iaIXtZY4hUEOWMUG2JINmdGy|IHH0JFExKHWPIHHmeIVzKDNiaILzJIJ6KESFRlitSGEhe3SjaX7pcocu[mG|ZXSg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweGmlIHHuZYx6e2m| MmHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
HCT116 MonkRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MYi4JJVO NUP1[o9pPzJiaILz NVOwbGFuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XRiODD1UUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5 NYj6SGh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NlQ2OzlpPkK5OFI1PTN7PD;hQi=>
HCT116 NHm5bJpCdnSraX72ZZNqfmViYYPzZZk> MUK1NEBvVQ>? M2\YNVQ5KGi{cx?= NV7hVFRbSW62aXnueoF{cX[nIHHjeIl3cXS7IHnuJIh2dWGwIFjDWFEyPiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHRITi2kZYThNU1qdmS3Y3XkJINmdGxiaX72ZZNqd25iYYSgOVAhdk1iYX\0[ZIhPDhiaILzJIJ6KGO{eYP0ZYwhfmmxbHX0JJN1[WmwaX7nMYJie2WmIHHzd4F6 Mn3FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
HCT116 MkDCSpVv[3Srb36gZZN{[Xl? NIPWe5A2OCCwTR?= MUCxJIhz NXTjSXF2UW6qaXLpeIlwdiCxZjDJUE0y[mW2YT3t[YRq[XSnZDDh[Ihme2mxbjDv[kBpfW2jbjDybI9l[W2rbnWgNVI{NWyjYnXs[YQhUEOWMUG2JINmdGy|IITvJGhWXkWFIHH0JFUxKG6PIHHmeIVzKDFiaIKgZpkh\my3b4Lld4Vv[2ViYYPzZZk> M1XZTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
HCT116 NUTvSGlvTnWwY4Tpc44h[XO|YYm= M4L1OFUxKG6P M4T4RlQ5KGi{cx?= NEe4WXNKdmirYnn0bY9vKG:oIGTHSk1j\XSjMT3pcoR2[2WmIHTlZ5Jm[XOnIHnuJIV5eHKnc4Ppc44hdGW4ZXzzJI9nKEVvY3HkbIVzcW5icILveIVqdiCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZQhPTBibl2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> M4TSTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
HCT116 M{DRTGZ2dmO2aX;uJIF{e2G7 MkewOVAhdk1? NVrKW5RPPDhiaILz M4DjRmlvcGmkaYTpc44hd2ZiVFfGMYJmfGFzLXnu[JVk\WRiaX7jdoVie2ViaX6g[ZhxemW|c3nvckBt\X[nbIOgc4YhfmmvZX70bY4heHKxdHXpckBqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYYSgOVAhdk1iYX\0[ZIhPDhiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NEjmdXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSyOFU{QSd-Mkm0NlQ2Ozl:L3G+
HCT116 M3jWS2Z2dmO2aX;uJIF{e2G7 MYi1NEBvVQ>? MVi0PEBpenN? MWXJcohq[mm2aX;uJI9nKFSJRj3i[ZRiOS2rbnT1Z4VlKGmwY4LlZZNmKGmwIHX4dJJme3Orb36gcIV3\Wy|IH;mJG1OWC17IIDyc5RmcW5iaX6gbJVu[W5iSFPUNVE3KGOnbHzzJIF1KDVyIH7NJIFnfGW{IES4JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NYnBZ2RWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NlQ2OzlpPkK5OFI1PTN7PD;hQi=>
HCT116 MkPISpVv[3Srb36gZZN{[Xl? MkX0OVAhdk1? M1HY[VQ5KGi{cx?= NGLRRllKdmirYnn0bY9vKG:oIGTHSk1j\XSjMT3pcoR2[2WmIHnuZ5Jm[XOnIHnuJIV5eHKnc4Ppc44hdGW4ZXzzJI9nKHOwYXnsJJBzd3SnaX6gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG2IEWwJI5OKGGodHXyJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
HCT116 M1OxVWZ2dmO2aX;uJIF{e2G7 MlLQOVAhdk1? MUe0PEBpenN? M2fUTGlvcGmkaYTpc44hd2ZiVFfGMYJmfGFzLXnu[JVk\WRiaX7jdoVie2ViaX6g[ZhxemW|c3nvckBt\X[nbIOgc4YhfHerc4SgdJJwfGWrbjDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XRiNUCgcm0h[W[2ZYKgOFghcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
HCT116 M{jSSmZ2dmO2aX;uJIF{e2G7 MVi1NEBvVQ>? NWXiZlZKPDhiaILz MoLoTY5pcWKrdHnvckBw\iCWR1[tZoV1[TFvaX7keYNm\CCDS2SgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIFjDWFEyPiClZXzsd{BifCB3MDDuUUBi\nSncjC0PEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M1TIR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
HCT116 MlGxSpVv[3Srb36gZZN{[Xl? MmjtOVAhdk1? NWCxd4ZZPDhiaILz M4Xs[GlvcGmkaYTpc44hd2ZiVFfGMYJmfGFzLXnu[JVk\WRiR2PLMVNj\XSjIIDoc5NxcG:{eXzheIlwdiCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZQhPTBibl2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR{NEWzPUc,Ojl2MkS1N|k9N2F-
HCT116 NHPBdXVHfW6ldHnvckBie3OjeR?= NH65PHA2OCCwTR?= MUO0PEBpenN? Mlu1TY5pcWKrdHnvckBw\iCWR1[tZoV1[TFvcnXneYxifGWmIHTlZ5Jm[XOnIHnuJIN6fG:ybHHzcYlkKGKndHGtZ4F1\W6rbjDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XRiNUCgcm0h[W[2ZYKgOFghcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MoO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2MkS1N|koRjJ7NEK0OVM6RC:jPh?=
HCT116 M4XjW2Z2dmO2aX;uJIF{e2G7 M3G2fVUxKG6P MVW0PEBpenN? MnX6TY5pcWKrdHnvckBw\iCWR1[tZoV1[TFvcnXneYxifGWmIHnuZ5Jm[XOnIHnuJI52[2ynYYKgZoV1[S2lYYTlcolvKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDheEA2OCCwTTDh[pRmeiB2ODDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M2Gwe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEK0OVM6Lz5{OUSyOFU{QTxxYU6=
DU145 MWnGeY5kfGmxbjDhd5NigQ>? NIrBN2QzPCCqcoO= M4mwV2lvcGmkaYTpc44hd2ZiU2TBWFMheGixc4Doc5J6dGG2aX;uJIF1KFS7ckewOUBz\XOrZIXld{BqdiCqdX3hckBFXTF2NTDj[YxteyCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m|LDDJR|UxRTgQvF2= M3rZNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OEC3O|k2Lz5{OUiwO|c6PTxxYU6=
DU145 M{O1UGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDMUlBvPDhiaILz NX;NZXN1T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFeVVD2xJIF{e2G7LDDHTVUxRTdwMd88US=> NH7qO3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUiwO|c6PSd-Mkm4NFc4QTV:L3G+
LNCAP NF;qPWdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWi0PEBpenN? MlflS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUG5ESVBiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHdUXC1zIHHzd4F6NCCJSUWwQVkvOs7:TR?= NFvRdlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUiwO|c6PSd-Mkm4NFc4QTV:L3G+
WI38 M1LUU2Z2dmO2aX;uJIF{e2G7 NETaPFlU\W6xbIn0bYMh[WO2aY\peJkh[WejaX7zeEBqd26rennu[{Bz[WSrYYTpc44hcW6mdXPl[EBpfW2jbjDXTVM5KHOnbnXzZ4VvfCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfUwhTUN3ME24{txO MojiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl7MkW0PFQoRjJ7OUK1OFg1RC:jPh?=
WI38 MVLGeY5kfGmxbjDhd5NigQ>? M{PPcnNmdm:ueYTpZ{Bi[3Srdnn0fUBi\2GrboP0JI5wdi2|ZX7ld4NmdnRiaIXtZY4hX0l|ODDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZpkheHKxcHnkbZVuKGmxZHnk[UB{fGGrbnnu[{Bj[XOnZDDmcI94KGO7dH;t[ZRzgSxiRVO1NF0zOC5|zszN M3rmZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUK1OFg1Lz5{OUmyOVQ5PDxxYU6=
WI38 MV7GeY5kfGmxbjDhd5NigQ>? NVXDbmxOPSC3TR?= NUWxTG9mOjRiaILz M2H4WWlv\HWldHnvckBw\iCRWGKxJIRm\3KjZHH0bY9vKGmwIHnvcol7cW6pIILh[IlifGmxbjDpcoR2[2WmIHj1cYFvKFeLM{igd4Vv\XOlZX70JINmdGy|IHH0JFUhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl{NUS4OEc,Ojl7MkW0PFQ9N2F-
WI38 NWXnPWxWTnWwY4Tpc44h[XO|YYm= MljUeZAhfG9iNkSgeW0> MXmxMlUhcHK| NI\OSG5KdmS3Y4Tpc44hd2ZiUl;TJJBzd2S3Y4Tpc44hcW5iaX;ubZpqdmdicnHkbYF1cW:wIHnu[JVk\WRiaIXtZY4hX0l|ODDz[Y5me2OnboSgZ4VtdHNidYCgeI8hPjRidV2gZYZ1\XJiMT61JIhzeyCkeTDETHIyOjNiZInlJIJie2WmIH\sc5ch[3m2b33leJJ6 M{PQSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUK1OFg1Lz5{OUmyOVQ5PDxxYU6=
RAW264.7 MYfGeY5kfGmxbjDhd5NigQ>? NFPwN2UyOCC3TR?= MUKwMlUhcHK| MkXjTY5pcWKrdHnvckBw\iCPQWDLM25Hc2GycHHCJIlvKEmFUjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUNWmwZIXj[YQhXE6IYXzwbIEheHKxZIXjeIlwdiCjdDCxNEB2VSCycnX0doVifGWmIH\vdkAxNjViaILzJIZwdGyxd3XkJIJ6KEySUzDjbIFtdGWwZ3WgZY5lKG2nYYP1doVlKGGodHXyJFI1KGi{czDifUBGVEmVQTDy[YxifGm4ZTD0c{Bkd262cn;s NIrwcVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEe4NFA5QCd-M{C3PFAxQDh:L3G+
RAW264.7 NVrub45nTnWwY4Tpc44h[XO|YYm= MUexNEB2VQ>? M{LKdVAvPSCqcoO= MlPHTY5pcWKrdHnvckBw\iCPQWDLM25Hc2GycHHCJIlvKEmFUjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUNWmwZIXj[YQhUUx4IIDyc4R2[3Srb36gZZQhOTBidV2gdJJmfHKnYYTl[EBnd3JiMD61JIhzeyCob3zsc5dm\CCkeTDMVHMh[2ijbHzlcodmKGGwZDDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliRVzJV2EhemWuYYTpeoUhfG9iY3;ueJJwdA>? NW\odolwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PFAxQDhpPkOwO|gxODh6PD;hQi=>
BHK21 MofaR5l1d3SxeHnjbZR6KGG|c3H5 MVK0PEBpenN? NEL0[|REgXSxdH;4bYNqfHliYXfhbY5{fCCESFuyNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME20NE4yPM7:TR?= Mny1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyMEm5NFgoRjNzMEC5PVA5RC:jPh?=
SH-SY5Y M4j5VmN6fG:2b4jpZ4l1gSCjc4PhfS=> NGfBPY04OiCqcoO= MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTTE1UYTW\IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS57NN88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTRyMEewPEc,OzF2MEC3NFg9N2F-
U87 M4L3UGN6fG:2b4jpZ4l1gSCjc4PhfS=> MUS3NkBpenN? MkHRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVg4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9O{4yQM7:TR?= NXnW[GhCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G0NFA4ODhpPkOxOFAxPzB6PD;hQi=>
SH-SY5Y MV7GeY5kfGmxbjDhd5NigQ>? MnL2NVUhdWmwcx?= M1TKcGlvcGmkaYTpc44hd2ZiVIL4VkBqdiCqdX3hckBUUC2VWUXZJINmdGy|IIXzbY5oKESWTlKgZZMhe3Wkc4TyZZRmKHC{ZXnuZ5Vj[XSnZDDmc5IhOTVibXnud{Bnd2yub4fl[EBjgSC|dXLzeJJifGViYXTkbZRqd25iYX7kJI1m[XO3cnXkJIZweiB{MDDtbY5{KGK7IFXscI1idie|IH3leIhw\CxiSVO1NF01PS56ON88US=> NUDMWmNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G0NFA4ODhpPkOxOFAxPzB6PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
CDK2 / Cyclin E / Cyclin A ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for 12, 24, or 48 h; cyclins and CDK2 levels were then measured

Bax / Bcl-2 ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for 12 or 24 h and Bcl-2 and Bax levels were measured. Quantitative analysis was performed using Image J software, with normalization to GAPDH expression.

Survivin / p21 / p27 ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for different amounts of time. Survivin, p21, and p27 levels were then measured.

p-STAT3 / STAT3 / p-JAK2 / JAK2 / c-myc ; 

PubMed: 27634873     


NCI-H929 cells were treated with 4 μM piperlongumine for different amounts of time, and STAT3, p-STAT3, p-JAK2, JAK2 and c-myc levels were then measured.

Hexokinase 1 / Hexokinase 2 ; 

PubMed: 30906213     


Piperlongumine regulates glycolysis in NSCLC cells. NSCLC cells, including H23 (A), HCC827 (B) and H1975 (C) were treated with different concentrations of piperlongumine for 24 h. Western blotting was performed to detect HK2 expression (left). The levels of glucose consumption (middle) and lactate production (right) were examined in these cells. Columns, mean of the number of colonies as determined from three independent experiments; bars, standard deviation; asterisk, significant (*p<0.05, **p<0.01, ***p<0.001) suppression of HK2 expression or glycolysis by piperlongumine compared with DMSO treated group. PL, Piperlongumine.

p-AKT / AKT / p-S6 / S6 ; 

PubMed: 30906213     


HCC827 (left), H1975 (middle) and H23 cells were treated with piperlongumine for 24 h, western blot was conducted to detect the target proteins as indicated.

27634873 30906213
Growth inhibition assay
Cell viability; 

PubMed: 25193861     


Piperlongumine induces death in HNC cells but not normal cells. Cytotoxicity was assessed by MTT assay 

25193861
In vivo Piperlongumine (50 mg/kg i.p.) causes in vivo growth inhibition of tumor cells without leading to major changes in the biochemical, hematological and histopathological parameters. [4]

Protocol

Cell Research:

[3]

- Collapse
  • Cell lines: MCF-7 and 786-O cells
  • Concentrations: ~10 μM
  • Incubation Time: 48 hours
  • Method:

    MCF-7 and 786-O cells are incubated with various PL concentrations for 48h. Cell proliferation is analysed by CellTiter Blue assay. Effective doses (ED) are calculated using XLift, Microsoft Excel add-in.


    (Only for Reference)
Animal Research:

[4]

- Collapse
  • Animal Models: Mice transplanted with sarcoma 180 tumors
  • Dosages: ~50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 16 mg/mL warmed (50.41 mM)
Ethanol 6 mg/mL warmed (18.9 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 317.34
Formula

C17H19NO5

CAS No. 20069-09-4
Storage powder
in solvent
Synonyms PPLGM, Piplartine
Smiles COC1=CC(=CC(=C1OC)OC)C=CC(=O)N2CCC=CC2=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Piperlongumine | Piperlongumine supplier | purchase Piperlongumine | Piperlongumine cost | Piperlongumine manufacturer | order Piperlongumine | Piperlongumine distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID