Catalog No.S7551 Synonyms: PPLGM, Piplartine

Piperlongumine Chemical Structure

Molecular Weight(MW): 317.34

Piperlongumine, a natural alkaloid from Piper longum L., increases the level of reactive oxygen species (ROS) and selectively kills cancer cells. It is a direct TrxR1 inhibitor with suppressive activity against gastric cancer and a novel inhibitor of CRM1; also an inhibitor of PI3K/Akt/mTOR in human breast cancer cells.

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  • a. Cell growth of ARID1A-wildtype RMG1 cells transfected with ARID1A and non-target siRNA for 24 h and treated with piperlongumine for 72 h. b. Apoptosis of RMG1 cells after transfection and treatment as described in a as measured using annexin-V and PI staining. c. Cell growth of RMG1 cells transfected and treated with 5 μM of piperlongumine as described in a, but in the presence or absence of the antioxidant NAC. Cell growth was measured using the WST-1 assay and quantified relative to DMSO treated non-target control. *P < 0.05; **P < 0.01; ***P < 0.001.

    Oncotarget, 2016, 7(35):56933-56943. Piperlongumine purchased from Selleck.

    MPC cells were treated with the indicated concentrations of PL at 21% and 1% O2 for 24 hours. Total cell lysates were subjected to Western blot with antibodies against cleaved PARP, cleaved caspase 3, and caspase 3. β-tubulin was used as a loading control. A representative image (n=3) is shown.

    Oncotarget, 2016, 7(26):40531-40545. Piperlongumine purchased from Selleck.

Purity & Quality Control

Choose Selective ROS Inhibitors

Biological Activity

Description Piperlongumine, a natural alkaloid from Piper longum L., increases the level of reactive oxygen species (ROS) and selectively kills cancer cells. It is a direct TrxR1 inhibitor with suppressive activity against gastric cancer and a novel inhibitor of CRM1; also an inhibitor of PI3K/Akt/mTOR in human breast cancer cells.
reactive oxygen species (ROS) [1] TrxR1 [5] CRM1 [6] PI3K/Akt/mTOR [7]
In vitro

Piperlongumine is a known ROS inducer which could induce pancreatic cancer cell death in cell culture[1] As a thromboxane A(2) receptor antagonist, Piperlongumine inhibits platelet aggregation. [2] Piperlongumine also promotes autophagy via inhibition of Akt/mTOR signalling and mediates cancer cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MA9.3 cells MYrDfZRwfG:6aXPpeJkh[XO|YYm= MlHoOFghcA>? NGfYR5NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOSTlwMzDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVIh|ryP NF\iVYkzPTR4NEi4Oy=>
mouse RAW264.7 cells M4TWfWZ2dmO2aX;uJIF{e2G7 NIjXSVBCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdixiSVO1NF0{KM7:TR?= M1PmdFI2PDV|OEC5
PANC1 cells NXXzeXlqS3m2b4TvfIlkcXS7IHHzd4F6 NX3rXGM4OjRiaB?= MkjJR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGSgdoVlfWO2aX;uJIF{e2G7LDDJR|UxRTNwMjFOwG0> M3i3ZlI2OzB3N{G4
SK-MEL-2 cells NFT2VnlRem:uaX\ldoF1cW:wIHHzd4F6 NFrmbnQ4OiCq Ml7GRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSz3NSWwuOiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBGSzVyPUSuN|kh|ryP NGLLVpQzPTh{NkO5PC=>
HaCaT cells MlrHVJJwdGmoZYLheIlwdiCjc4PhfS=> MYe3NkBp NHrq[2pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjhR4FVKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;ND60OkDPxE1? MoTBNlU5OjZ|OUi=
ZR75-30 cells NGXYVJBEgXSxdH;4bYNqfHliYYPzZZk> Mm\nO|IhcA>? NIH6emFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBbWjd3LUOwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS56NjFOwG0> NXizbWJFOjR7M{exPFY>
HCT116 cells M2fCRmN6fG:2b4jpZ4l1gSCjc4PhfS=> Ml;GO|IhcA>? NGXjdWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF03NjB2IN88US=> NIH1bmgzPDl|N{G4Oi=>
A375 cells M2LSNXBzd2yrZnXyZZRqd25iYYPzZZk> NYCxUm5VPzJiaB?= MlHhRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDM{e1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Pi5zNzFOwG0> NVn1SZB1OjV6Mk[zPVg>
MDA-MB-231 cells M2XNTGN6fG:2b4jpZ4l1gSCjc4PhfS=> MnzjO|IhcA>? Mlr4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRThwNE[g{txO M{K1fFI1QTN5MUi2
A549 cells MYfQdo9tcW[ncnH0bY9vKGG|c3H5 MYG3NkBp M1XvcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTF{LkKg{txO M33DZVI2QDJ4M{m4
HFF1 cells NWP4Z2lDWHKxbHnm[ZJifGmxbjDhd5NigQ>? NXHMeYRVPzJiaB?= MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFjGSlEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1zMz6xJO69VQ>? MoLCNlU5OjZ|OUi=
MCF10A cells NHnnNYVRem:uaX\ldoF1cW:wIHHzd4F6 M4\CfVczKGh? MWPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkGwRUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTF6LkGg{txO MYSyOVgzPjN7OB?=
WI38 cells MnHHR5l1d3SxeHnjbZR6KGG|c3H5 Mke1O|IhcA>? NYm2eJJqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hX0l|ODDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI3Njd6IN88US=> NIfEfGgzPDl|N{G4Oi=>
MRC5 cells MlnZR5l1d3SxeHnjbZR6KGG|c3H5 MUS3NkBp M2r5PWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2zOU4xPCEQvF2= MkHaNlQ6OzdzOE[=

... Click to View More Cell Line Experimental Data

In vivo Piperlongumine (50 mg/kg i.p.) causes in vivo growth inhibition of tumor cells without leading to major changes in the biochemical, hematological and histopathological parameters. [4]


Cell Research:


+ Expand
  • Cell lines: MCF-7 and 786-O cells
  • Concentrations: ~10 μM
  • Incubation Time: 48 hours
  • Method:

    MCF-7 and 786-O cells are incubated with various PL concentrations for 48h. Cell proliferation is analysed by CellTiter Blue assay. Effective doses (ED) are calculated using XLift, Microsoft Excel add-in.

    (Only for Reference)
Animal Research:


+ Expand
  • Animal Models: Mice transplanted with sarcoma 180 tumors
  • Formulation: 4% DMSO
  • Dosages: ~50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 16 mg/mL warmed (50.41 mM)
Ethanol 6 mg/mL warmed (18.9 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 317.34


CAS No. 20069-09-4
Storage powder
in solvent
Synonyms PPLGM, Piplartine

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID