Asiaticoside

Catalog No.S3616 Synonyms: Ba 2742, BRN0078195, CCRIS8995, NSC166062, Emdecassol,Madecassol

For research use only.

Asiaticoside (Ba 2742, BRN0078195, CCRIS8995, NSC166062, Emdecassol,Madecassol), the major active principle of Centella asiatica, prevents ultraviolet A-dependent photoaging by suppressing ultraviolet A-induced reactive oxygen species production. It also decreases DNA binding by MITF.

Asiaticoside Chemical Structure

CAS No. 16830-15-2

Selleck's Asiaticoside has been cited by 1 Publication

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Biological Activity

Description Asiaticoside (Ba 2742, BRN0078195, CCRIS8995, NSC166062, Emdecassol,Madecassol), the major active principle of Centella asiatica, prevents ultraviolet A-dependent photoaging by suppressing ultraviolet A-induced reactive oxygen species production. It also decreases DNA binding by MITF.
In vitro

In the central nervous system, Asiaticoside has been shown to attenuate in vitro neuronal damage caused by exposure to β-amyloid. In cultured mouse cortical neurons exposed to glutamate-induced excitotoxicity invoked by N-methyl-D-aspartate, pretreatment with Asiaticoside decreases neuronal cell loss in a con­centration-dependent manner and restored changes in expression of apoptotic-related proteins Bcl-2 and Bax. Asiaticoside pretreatment also attenuates the upregulation of NR2B expression, a subunit of N-methyl-D-aspartate receptors, but does not affect expression of NR2A subunits. It also significantly inhibits Ca 2+ influx induced by N-methyl-D-aspartate[1]. Presence of asiaticoside along with the DNA during irradiation prevents the relaxation of the supercoiled form to the open circular form[2].

In vivo In vivo studies demonstrate that Asiaticoside could attenu­ate neurobehavioral, neurochemical and histological changes in transient focal middle cerebral artery occlusion animals. In addition, Asiaticoside shows anxiolytic effects in acute and chronic stress animals. It has been shown to have wound healing effects, anti-infammatory effects, and promotes liver protective activity[1]. Administration of asiaticoside prior to whole-body radiation exposure of the mice prevents this increase in radiation-induced increase in comet parameters, which could be the result of protection to DNA under in vivo conditions of radiation exposure[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Cortical neurons
  • Concentrations: 0.1, 1, 10, 100 μM
  • Incubation Time: 24 h
  • Method: Cortical neurons cultured for 7 days in vitro are divided into three groups: control, NMDA treatment (200 μmol/L NMDA and 10 μmol/L glycine, NMDA receptor co-activa­tor) and Asiaticoside pretreatment (Asiaticoside (less than 1%) pretreatment for 24 hours, then NMDA (200 μmol/L) and glycine (10 μmol/L) treatment for another 30 minutes). The NR2B receptor specific antagonist, RO-25-6981, is used as a positive control. The cells are then returned to the original culture medium for further 24 hours.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 959.12
Formula

C48H78O19

CAS No. 16830-15-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)O)O)C)C)C2C1C)C)C(=O)OC6C(C(C(C(O6)COC7C(C(C(C(O7)CO)OC8C(C(C(C(O8)C)O)O)O)O)O)O)O)O

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03814850 Not yet recruiting Device: Standard Manual Aneroid Sphygmomanometer Unruptured Cerebral Aneurysm Sebastian Koch|University of Miami December 1 2022 Not Applicable
NCT05157724 Not yet recruiting Procedure: PLASMA Prostatic Hyperplasia Benign|Prostatic Adenoma Elsan October 2022 --
NCT05443061 Not yet recruiting Drug: Nitroglycerin|Drug: Normal saline Arterial Line|Ischemia Seoul National University Hospital September 1 2022 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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