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Pyrogallol

Synonyms: 1,2,3-trihydroxybenzene

Pyrogallol (1,2,3-trihydroxybenzene), an organic compound belonging to the phenol family, used as a photographic film developer and in the preparation of other chemicals. It is known to be a superoxide anion generator and ROS inducer.

Pyrogallol Chemical Structure

Pyrogallol Chemical Structure

CAS: 87-66-1

Selleck's Pyrogallol has been cited by 3 publications

Purity & Quality Control

Batch: S388501 DMSO] 25 mg/mL] false] ] ] false] ] ] false Purity: 99.99%
99.99

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Biological Activity

Description Pyrogallol (1,2,3-trihydroxybenzene), an organic compound belonging to the phenol family, used as a photographic film developer and in the preparation of other chemicals. It is known to be a superoxide anion generator and ROS inducer.
In vitro
In vitro Pyrogallol (PG) inhibits the growth of human pulmonary A549 cells by inducing cell cycle arrest as well as triggering apoptosis. PG can disturb the natural oxidation and reduction equilibrium in cells. Oxidative stress by PG induces NF kappa B DNA binding and inducible NOS mRNA in cultured A549 lung epithelial cells, which may play a role in defending cells against PG-induced oxidative stress[1].
Cell Research Cell lines A549 cells
Concentrations 0-100 μM
Incubation Time 72 h
Method

1×106 cells are incubated with 0-100 μM of PG for 72 h. Cells are washed twice with cold PBS and then resuspended in 500 μl of binding buffer (10 mM HEPES/NaOH pH 7.4, 140 mM NaCl, 2.5 mM CaCl2) at 1×106 cells/ml. Then 5 μl of annexin V-FITC is added to these cells, which are analyzed with a FACStar flow cytometer.
In Vivo
In vivo Dermal administration of pyrogallol causes increased incidences of nonneoplastic lesions of the skin at the site of application in male and female rats and mice, skin adjacent to the site of application in male and female mice, and mammary gland in female mice[2].
Animal Research Animal Models Male and female F344/N rats and B6C3F1/N mice
Dosages 0, 9.5, 18.75, 37.5, 75, or 150 mg/kg (rats); 0, 38, 75, 150, 300, or 600 mg/kg (mice)
Administration dermally

Chemical Information & Solubility

Molecular Weight 126.11 Formula

C6H6O3
CAS No. 87-66-1 SDF Download Pyrogallol SDF
Smiles C1=CC(=C(C(=C1)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 25 mg/mL ( (198.23 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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