Necrostatin 2 racemate (Nec-1s)

Catalog No.S8641 Synonyms: 7-Cl-O-Nec1, Necrostatin-1 stable, Necrostatin 1S

For research use only.

Necrostatin 2 racemate (Nec-1s, 7-Cl-O-Nec1, Necrostatin-1 stable, Necrostatin 1S) is a stable variant of Necrostatin-1. It is a more specific RIPK1 inhibitor lacking the IDO-targeting effect, with >1000-fold more selective for RIPK1 than for any other kinase out of 485 human kinases.

Necrostatin 2 racemate (Nec-1s) Chemical Structure

CAS No. 852391-15-2

Selleck's Necrostatin 2 racemate (Nec-1s) has been cited by 38 publications

Purity & Quality Control

Choose Selective RIP kinase Inhibitors

Biological Activity

Description Necrostatin 2 racemate (Nec-1s, 7-Cl-O-Nec1, Necrostatin-1 stable, Necrostatin 1S) is a stable variant of Necrostatin-1. It is a more specific RIPK1 inhibitor lacking the IDO-targeting effect, with >1000-fold more selective for RIPK1 than for any other kinase out of 485 human kinases.
Targets
RIPK1 [1]
In vitro

Nec-1s is a potent inhibitor of RIPK1 and cellular necroptosis while lacking IDO inhibitory activity[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U118 cells MYnGeY5kfGmxbjDhd5NigQ>? M1rDPFIxOCEQvF2= M4Dwbm5m[y1zczDy[YR2[2W|IGDJJIlv[2:{cH;yZZRqd25iaX6g[YRmdG[xc3nu[U11emWjdHXkJHUyOThiY3XscJMv M3zhbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUmzPVk1Lz5{NUW5N|k6PDxxYU6=
MEFs NWryc2RwTnWwY4Tpc44h[XO|YYm= NFrWUnczOCEEtV2= MnfuNVAhdWmw NH2yS3pVcGViY4nsbY5lem:vYYTvd4l{KGOuZXH2ZYdmKGmwZIXj[YQh[nliVF7G{tEh[2:3bHSgZoUhcW6qaXLpeIVlKGK7IF7lZ{0yey5? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDdyMUO3OUc,Ojh5MEGzO|U9N2F-
Jurkat MUjGeY5kfGmxbjDhd5NigQ>? NEO5Toc{OCCqcoO= NH63UZpKdmirYnn0bY9vKG:oIHTlZZRpKHKnY3XweI9zKHOrZ37hcIlv\yCvZXTpZZRm\CCwZXPyc5B1d3SrYzDj[YxtKGSnYYToJIlvKGi3bXHuJGp2emujdDDj[YxtKGSnZnnjbYVvfCCrbjDGRWRFKGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFMxKGi{czDifUBCXFBiYnHz[YQhfmmjYnnsbZR6KGG|c3H5JIlvKHC{ZYPlcoNmKG:oIGTOSoFteGijLDDJR|UxKD1iMD6yNFYh|ryPLh?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjRyOECwPEc,OTZ2MEiwNFg9N2F-
Jurkat Moj3SpVv[3Srb36gZZN{[Xl? NYnqNoZOTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSg[o9zKGmwaHnibZRqd25ib3[gcoVkem:ydH;zbZMhcW5iRlHESEBl\W[rY3nlcpQhUnW{a3H0JHQh[2WubIOgeJJm[XSnZDD3bZRpKFSQRj3hcJBp[SxiRVO1NEA:KDBwMkGg{txONg>? M2HMUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MUWzPFQxLz5zNkG1N|g1ODxxYU6=
Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 29527209
In vivo

Nec-1s is effective in reducing brain injuries. It is a superior inhibitor suitable for use in vivo lacking a paradoxical sensitizing effect in TNF-induced lethality[1]. Nec-1s possesses several advantageous pharmacokinetic and pharmacodynamic characteristics in comparison to Nec-1. Safety profile of Nec-1s is also superior to that of Nec-1 for reduced in vivo and in vitro toxicity[2].

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: TNF-induced SIRS model (Female C57BL/6J WT mice)
  • Dosages: 0.6 mg/kg and 6 mg/kg
  • Administration: i.v.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 277.71
Formula

C13H12ClN3O2

CAS No. 852391-15-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CN1C(=O)C(NC1=O)CC2=CNC3=C2C=CC=C3Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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