Home Apoptosis RIP kinase inhibitor - Autophagy activator ICCB-19 hydrochloride For research use only.

ICCB-19 hydrochloride

ICCB-19 hydrochloride is an inhibitor of TNFRSF1A Associated Via Death Domain (TRADD) with IC50 of 1.12 μM and 2.01 μM for protecting Velcade-induced apoptosis in Jurkat cells and protecting RDA in MEFs, respectively. ICCB-19 indirectly inhibits Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). ICCB-19 effectively induces autophagy.

ICCB-19 hydrochloride Chemical Structure

ICCB-19 hydrochloride Chemical Structure

CAS: 1803605-68-6

Selleck's ICCB-19 hydrochloride has been cited by 1 publication

Purity & Quality Control

Batch: S607801 DMSO] 58 mg/mL] false] Water] 58 mg/mL] false] Ethanol] 58 mg/mL] false Purity: 99.25%
99.25

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Biological Activity

Description ICCB-19 hydrochloride is an inhibitor of TNFRSF1A Associated Via Death Domain (TRADD) with IC50 of 1.12 μM and 2.01 μM for protecting Velcade-induced apoptosis in Jurkat cells and protecting RDA in MEFs, respectively. ICCB-19 indirectly inhibits Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). ICCB-19 effectively induces autophagy.
Targets
TRADD [1] RIPK1 [1]
In vitro
In vitro

ICCB-19 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N), which interacts with the C-terminal domain (TRADD-C) and TRAF2 to modulate the ubiquitination of RIPK1 and beclin 1.[2]
Cell Research Cell lines HEK293T cells, MEFs, Jurkat cells, SH-SY5Y cells, HT-29 cells, U-937 cells, H4 cells and HeLa cells
Concentrations 10 μM
Incubation Time --
Method

The rates of cell death were measured in triplicate or quadruplicate in a 96-well or 384-well plate using SYTOX Green Nucleic Acid Stain or ToxiLight Non-destructive Cytotoxicity BioAssay Kit. The intensity of luminescence was determined in an EnSpire Multimode Plate Reader. Cytotoxicity was expressed as percentages of cell death per well after deducting the background signal in non-induced cells and compared to that of the maximal cell death with 100% Lysis Reagent.

Chemical Information & Solubility

Molecular Weight 291.84 Formula

C12H22ClN3OS
CAS No. 1803605-68-6 SDF --
Smiles Cl.O=C(CSC1=NCCN1)NC2CCCCCC2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 58 mg/mL ( (198.73 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 58 mg/mL

Ethanol : 58 mg/mL


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In vivo
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