Home Apoptosis RIP kinase inhibitor Mito-TEMPO For research use only.

Mito-TEMPO

Mito-TEMPO (MT), a mitochondria-targeted superoxide dismutase mimetic, protects against the early phase of acetaminophen (APAP) hepatotoxicity by inhibiting peroxynitrite formation. Mito-TEMPO treatment inhibits APAP-induced RIP3 kinase expression.

Mito-TEMPO Chemical Structure

Mito-TEMPO Chemical Structure

CAS: 1334850-99-5

Selleck's Mito-TEMPO has been cited by 14 publications

Purity & Quality Control

Batch: S973301 DMSO] 100 mg/mL] false] Water] 100 mg/mL] false] Ethanol] 100 mg/mL] false Purity: 98.43%
98.43

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Biological Activity

Description Mito-TEMPO (MT), a mitochondria-targeted superoxide dismutase mimetic, protects against the early phase of acetaminophen (APAP) hepatotoxicity by inhibiting peroxynitrite formation. Mito-TEMPO treatment inhibits APAP-induced RIP3 kinase expression.
Targets
RIP3 kinase [1]
In vitro
In vitro

Mito-TEMPO improves blastocyst development by superoxide reduction in porcine embryos in vitro.[2]
Cell Research Cell lines Porcine embryos
Concentrations 0.1 μM
Incubation Time --
Method

Embryos are cultured in 50 μl drops porcine zygote medium-3 (PZM-3) with 3  mg/ml BSA at 38.5 °C under 5% CO2. After culturing 48 hours, cleaved embryos are transferred to 50  μl drop of fresh PZM-3 medium and cultured for 4 days. Blastocyst formation is evaluated after 6 days of culture. Mito-TEMPO (0.1 μM) is added to the culture medium during the IVC periods.
In Vivo
In vivo

Mito-Tempo (MT) treatment inhibits APAP-induced RIP3 kinase expression, and RIP3-deficient mice shows caspase activation and apoptotic morphology in hepatocytes analogous to MT treatment.[1]
Animal Research Animal Models 8-12 week-old male C57BL/6J mice
Dosages 20 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 510.03 Formula

C29H35N2O2P.Cl
CAS No. 1334850-99-5 SDF --
Smiles [Cl-].CC1(C)CC(CC(C)(C)N1[O])NC(=O)C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4
Storage (From the date of receipt) 3 years -20°C powder (seal)

In vitro
Batch:

DMSO : 100 mg/mL ( (196.06 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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