Catalog No.S1940 Synonyms: Fluoroquinolone

For research use only.

Levofloxacin (Fluoroquinolone) is a broad-spectrum antibiotic topoisomerase II and topoisomerase IV inhibitor, used to treat respiratory, urinary tract, gastrointestinal, and abdominal infections.

Levofloxacin Chemical Structure

CAS No. 100986-85-4

Selleck's Levofloxacin has been cited by 7 Publications

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Biological Activity

Description Levofloxacin (Fluoroquinolone) is a broad-spectrum antibiotic topoisomerase II and topoisomerase IV inhibitor, used to treat respiratory, urinary tract, gastrointestinal, and abdominal infections.
Topoisomerase II [1] Topoisomerase IV [1]
In vitro

Levofloxacin is active against most aerobic Gram-positive and Gram-negative organisms and demonstrates moderate activity against anaerobes.[1] Levofloxacin is two-fold more active than ciprofloxacin against Streptococcus pneumoniae and 2- to 4-fold more active than ciprofloxacin against Staphylococcus aureus, Xanthomonas maltophilia, and Bacteroides fragilis. Levofloxacin is two- to eightfold more active than ciprofloxacin against coagulase-negative staphylococci and Acinetobacter spp., although these improvements in potency may not be clinically relevant. Levofloxacin inhibits 90% of streptococci when it is used at concentrations of 1 mg/mL to 2 mg/mL. [2] Levofloxacin exhibits twofold greater inhibitory and bactericidal activities than ofloxacin against either extracellular or intracellular tubercle bacilli. [3] Levofloxacin has the least inhibitory effect on osteoblastic cell growth, with a 50% inhibitory concentration of approximately 80 mg/mL at 48 and 72 hours. Levofloxacin results in strong inhibition of calcium deposition, as determined on day 14 by alizarin red staining and biochemical analysis.[4] Levofloxacin inhibits glycosaminoglycan synthesis initially and DNA synthesis and mitochondrial function secondarily at actual arthropathic concentrations in cultured rabbit chondrocytes but that these changes are reversible and not enough to kill the cells. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NFO4ToJHfW6ldHnvckBie3OjeR?= MXGxMlUhdWmwcx?= NGXQc5JKdmirYnn0bY9vKG:oIHj1cYFvKE2DVFWxMY1m\GmjdHXkJGFUWCtidYD0ZYtmKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNU42KG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwQVE4NjlizszN Mn3ZNlMzPDFyMkm=
human 5637 cells MmTZSpVv[3Srb36gZZN{[Xl? MVOyNFAhfG9iM{CwJJVoN22u MkTFNVIhcA>? NG[zXIZCdnSrYnHjeIVzcWGuIHHjeIl3cXS7IHHnZYlve3RidYLvdIF1cG:pZX7pZ{BGe2OqZYLpZ4hq[SClb3zpJHVVUTh7IHnu[oVkfGWmIHnuJIh2dWGwIEW2N|ch[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iaX70doFk\WyudXzhdkBj[WO2ZYLpZYwhdGW4ZXygZZQhOjByIITvJFMxOCC3Zz;tcEBi\nSncjCxNkBpenNiYomgd4VzcWGuIHTpcJV1cW:wIH3leIhw\A>? MUCyNFI{OTN7MB?=
In vivo Levofloxacin is as efficacious as or more efficacious than that with ciprofloxacin in systemic as well as pyelonephritis infections in mice. Levofloxacin achieves higher concentrations in the serum and tissue of mice than does ciprofloxacin. [2]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 361.37


CAS No. 100986-85-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1COC2=C3N1C=C(C(=O)C3=CC(=C2N4CCN(CC4)C)F)C(=O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05441930 Not yet recruiting Drug: Levofloxacin Ocular Implant|Drug: Control Cataract PolyActiva Pty Ltd June 23 2022 Phase 1
NCT05117788 Recruiting -- Tuberculosis Multidrug-Resistant Foundation for Innovative New Diagnostics Switzerland|V. N. Karazin Kharkiv National University|Kenya Medical Research Institute|Universidad Peruana Cayetano Heredia|Bioneer Corporation February 23 2022 --
NCT04239326 Recruiting Diagnostic Test: targeted Next Generation Sequencing (tNGS) Tuberculosis Multidrug-Resistant Foundation for Innovative New Diagnostics Switzerland April 16 2021 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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