Digoxin

Catalog No.S4290

Digoxin   Chemical Structure

Molecular Weight(MW): 780.94

Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.

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Biological Activity

Description Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.
Targets
α2β3 [1] α2β1 [1] α1β1 [1]
42.8 nM(Ki) 58.7 nM(Ki) 268 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
TK10 cells M4DIW2N6fG:2b4jpZ4l1gSCjc4PhfS=> MnH4OFghcA>? NYrKeJhnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXEtzMDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvODF2NjFOwG0> NXO4NZBVOTZ|MEmzNVU>
MCF7 cells MU\DfZRwfG:6aXPpeJkh[XO|YYm= NXnWdFJ7PDhiaB?= MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MD6wNlQyKM7:TR?= MUKxOlMxQTNzNR?=
UACC62 cells MonRR5l1d3SxeHnjbZR6KGG|c3H5 MWS0PEBp M1jPdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0N4MjDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvODJ7NTFOwG0> MVKxOlMxQTNzNR?=
K562 cells NWDZS5d3S3m2b4TvfIlkcXS7IHHzd4F6 NIrCe|BEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGK7IGjUWEBie3OjeTygTWM2OD1yLkK4NkDPxE1? NH\LTngyPjNyOUOxOS=>
CC20 cells MUfDfZRwfG:6aXPpeJkh[XO|YYm= MYS3NkBp NEO4cWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBESzJyIHPlcIx{KGGodHXyJFczKGi{czDifUBHVUODIH3leIhw\CxiSVO1NF0xNjJ2IN88US=> NInQWXMyQTh7NEezNy=>
HCT116 cells M4PWXGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mn:zO|IhcA>? MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGZOS0FibXX0bI9lNCCLQ{WwQVAvOjdizszN NVzzN2dkOTl6OUS3N|M>
HT-29 cells NXvWNVg2S3m2b4TvfIlkcXS7IHHzd4F6 MXO3NkBp NY\6WGltS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE[PQ1GgcYV1cG:mLDDJR|UxRTFwNDFOwG0> NWi3cohxOTl6OUS3N|M>
human colon cancer cells NWPEOoJvS3m2b4TvfIlkcXS7IHHzd4F6 NGSzbpI4OiCq MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDjc4xwdiClYX7j[ZIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF\NR2EhdWW2aH;kMEBKSzVyPUCuNUDPxE1? NYD1fWRkOTl6OUS3N|M>
MCF7 cells M1TuT2N6fG:2b4jpZ4l1gSCjc4PhfS=> NFywS|E4OiCq MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyPUSuO{DPxE1? NE\UdHEzOjNzNkG2PC=>
human T47D cells NFvuS2JEgXSxdH;4bYNqfHliYYPzZZk> M4nkcVczKGh? MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyPUCuOlYh|ryP NXvUOWYxOjJ|MU[xOlg>
SK-BR-3 cells MXnDfZRwfG:6aXPpeJkh[XO|YYm= NFrxV5Y4OiCq NHH6RWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2EUj2zJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7LDDJR|UxRTFwMEig{txO NVfHeFhJOjJ|MU[xOlg>
Hs578Bst cells NEK5TYxEgXSxdH;4bYNqfHliYYPzZZk> Mon2O|IhcA>? M4nxc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh{PTd6QoP0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7LDDJR|UxRTBwMESg{txO MmL3NlI{OTZzNki=
human Hs578Bst cells NYXlR5ZHS3m2b4TvfIlkcXS7IHHzd4F6 MXO0NEB2VQ>? M2flRVczKGh? Mm\rR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJM2PziEc4SgZ4VtdHNiYYSgOFAhfU1iYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XlicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGlEPTB;NEeuO{DPxE1? NV\SfopGOjJ|MU[xOlg>
SK-MEL-28 cells M1nsOWN6fG:2b4jpZ4l1gSCjc4PhfS=> MXizJIRigXN? M4LqdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HTD2yPEBk\WyuczDh[pRmeiB|IHThfZMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMk[2JO69VQ>? MXWyN|cxPjByNR?=
human U373 cells MW\DfZRwfG:6aXPpeJkh[XO|YYm= MlT5N{Bl[Xm| NGDVXZBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWOzd|IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9NE4yPDZizqzt MmHQNlM4ODZyMEW=
human A549 cells MYPDfZRwfG:6aXPpeJkh[XO|YYm= NG\JbWM{KGSjeYO= NHfk[oVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9NE4xPSEQvF2= MlLvNlM4ODZyMEW=
human PC3 cells NIPJOFVEgXSxdH;4bYNqfHliYYPzZZk> MnTzN{Bl[Xm| NFX2ZVhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlA4PSEQvF2= MXqyN|cxPjByNR?=
MCF7 cells MYLDfZRwfG:6aXPpeJkh[XO|YYm= M{LUNFMh\GG7cx?= MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD60NlYh|ryP M2HqflI{PzB4MEC1
Hs683 cells NXTx[4NkS3m2b4TvfIlkcXS7IHHzd4F6 NV7oNFA5OyCmYYnz M4nMe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh{Pjh|IHPlcIx{KGGodHXyJFMh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9NE4xPCEQvF2= MlnNNlM4ODZyMEW=
CHO cells M1m2OWZ2dmO2aX;uJIF{e2G7 MkDDdGlEPTBidnHseYV{KG[xcjDzc4RqfW1iZnz1c5Jme2OnaX6gLFExKHWPKTD1dJRic2ViaX6gU2FVWDGEMT30doFve2[nY4Tl[EBEUE9iY3XscJMtKEmFNUC9N{4zOzV7NDFOwG0> MW[yN|U4OTRzNR?=
rat hepatocytes MlLTSpVv[3Srb36gZZN{[Xl? NUnVSVdxTHK3ZzD1dJRic2ViaX6gdoF1KGincHH0c4N6fGW|LDDLcV0xNjF6IN88US=> MlnvNlA6OjZ4MUm=
human hepatocytes M4XIfWZ2dmO2aX;uJIF{e2G7 NXfCUVlyOC5yNTD0c{AyOCC3TR?= NUK2OHFRTHK3ZzD1dJRic2ViaX6gd4Fv\HerY3itZ5VtfHW{ZXSgbJVu[W5iaHXwZZRw[3m2ZYOgZZQhOC5yNTD0c{AyOCC3TTDheEA{PyCmZXfDJIJ6KE2rY3jh[Yxqey2PZX70[Y4hc2mwZYTpZ5Mwe2OrboTpcIxifGmxbjDzdIVkfHKxc3PvdJktKEuvPUKuN|kh|ryP NFTmNpgzODl{Nk[xPS=>
CHO cells MmrHSpVv[3Srb36gZZN{[Xl? NXv5UnBSeEmFNUCgeoFtfWW|IH\vdkB{d2SrdX2g[ox2d3Knc3PlbY4hMDFyIIXNLUB2eHSja3WgbY4hV0GWUEHCN{11emGwc3\lZ5Rm\CCFSF:gZ4VtdHNuIFnDOVA:OTNwMUiyOkDPxE1? NXX6[3FkOjN3N{G0NVU>
human HeLa cells M4PkUmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{izNlQ5KGh? MleyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKuNkDPxE1? M3\0PVI3OTJ{N{ey
RKO-AS45-1 cells M4fNTWN6fG:2b4jpZ4l1gSCjc4PhfS=> NFnlNIY1QCCq NF30c5REgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTU09vQWO0OU0yKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD60NkDPxE1? NHPBWo4zPjF{Mke3Ni=>
Hs578T cells M{D2U2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mn\BO|IhcA>? MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDId|U4QFRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTldk1IdG9iYYPzZZktKEmFNUC9NE4zPTFizszN MW[yNlMyPjF4OB?=
Drosophila Schneider cells NWL4XIVVTnWwY4Tpc44h[XO|YYm= MXTBcpRi\2:waYP0JIFkfGm4aYT5JIF1KHS{YX7zZYN1cX[jdHnvckBld22jaX6gc4YhWk:UZ3HtcYF1KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFTyc5NweGirbHGgV4NpdmWrZHXyJINmdGy|IHPvMYV5eHKnc4PpcochT2GuND3EUmEh[mmwZHnu[{Bld22jaX6sJGlEPTB;MjFOwG0> NXXoeZZ1OjRyNEC0PFY>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Src / Src / p-EGFR / EGFR / p-STAT3 / STAT3 ; 

PubMed: 25955608     


Analysis of phosphorylated and non-phosphorylated Src, EGFR and STAT3 in A549 cells in a dose-dependent manner. The cancer cells treated with or without various concentrations of digoxin (50, 100, 250 and 500 nM) or 100 nM dasatinib for 24 h were analyzed by Western blot. 

p-PI3K / PI3K / p-AKT / AKT / p-MEK / MEK / p-ERK / ERK / p-FAK / FAK / p-SAPK/JNK / p-Paxillin / Paxillin / p-p130Cas / p130Cas ; 

PubMed: 25955608     


Western blotting analyses of phosphorylated and non-phosphorylated PI3K, AKT, MEK1/2, ERK, FAK, SAPK/JNK, paxillin and p130Cas in (a) A549 cell lines after a 24-h treatment with 100, 250 or 500 nM digoxin. The solvent control was 0.1% DMSO; GAPDH was used as an internal control. 

Nrf2; 

PubMed: 30735911     


Digoxin inhibited the Nrf2 signaling in SW1990/Gem and Panc-1/Gem cells. (A–B) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells. (C–D) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells at different time points.

25955608 30735911
Growth inhibition assay
Cell viability; 

PubMed: 25955608     


Five lung cancer cell lines, (a) A549, (b) H3255, (c) H1975, (d) PC9 and (e) PC9/gef, were treated with 6 different doses (0, 0.01, 0.05, 0.1, 1 and 5 μM) of digoxin at different time points (0, 24, 48, 72 and 96 h), and cell viability was measured using the PrestoBlue Cell Viability reagent after treatment. Quantitative data are presented as the mean±SD (n = 3); *p<0.05 compared with solvent control (0.1% DMSO) at the corresponding time point.

25955608

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (128.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 780.94
Formula

C41H64O14

CAS No. 20830-75-5
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03559868 Not yet recruiting Drug: Digoxin|Other: Placebo Inflammatory Response Yale University August 20 2019 Phase 1
NCT03877965 Enrolling by invitation Drug: Digoxin Congenital Heart Disease Christoph P Hornik MD MPH|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|The Emmes Company LLC|Duke University August 5 2019 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID