Digoxin

Catalog No.S4290

Digoxin   Chemical Structure

Molecular Weight(MW): 780.94

Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.

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Biological Activity

Description Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.
Targets
α2β3 [1] α2β1 [1] α1β1 [1]
42.8 nM(Ki) 58.7 nM(Ki) 268 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
TK10 cells NISxXVlEgXSxdH;4bYNqfHliYYPzZZk> MV[0PEBp NVPZZ3hiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXEtzMDDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvODF2NjFOwG0> MUSxOlMxQTNzNR?=
MCF7 cells NY\RVmRTS3m2b4TvfIlkcXS7IHHzd4F6 NV:3RZNnPDhiaB?= NVX6[ZdSS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvODJ2MTFOwG0> MnjINVY{ODl|MUW=
UACC62 cells M4LhWGN6fG:2b4jpZ4l1gSCjc4PhfS=> M13NOlQ5KGh? NFri[5NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWSUOFNkKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjB{OUWg{txO NVHYe|JbOTZ|MEmzNVU>
K562 cells MnLHR5l1d3SxeHnjbZR6KGG|c3H5 M33wU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGs2PjJiY3XscJMh[nliWGTUJIF{e2G7LDDJR|UxRTBwMkiyJO69VQ>? MXexOlMxQTNzNR?=
CC20 cells NIfNeI5EgXSxdH;4bYNqfHliYYPzZZk> M4fMSVczKGh? Ml[yR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2MzOCClZXzsd{Bi\nSncjC3NkBpenNiYomgSm1ESSCvZYToc4QtKEmFNUC9NE4zPCEQvF2= MWGxPVg6PDd|Mx?=
HCT116 cells M1u5XWN6fG:2b4jpZ4l1gSCjc4PhfS=> NVPP[IVPPzJiaB?= MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGZOS0FibXX0bI9lNCCLQ{WwQVAvOjdizszN NV7v[FQ5OTl6OUS3N|M>
HT-29 cells NF:xbVlEgXSxdH;4bYNqfHliYYPzZZk> NG\QZZU4OiCq M37xXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFczKGi{czDifUBHVUODIH3leIhw\CxiSVO1NF0yNjRizszN M3XkXVE6QDl2N{Oz
human colon cancer cells NEizfJhEgXSxdH;4bYNqfHliYYPzZZk> NXSxNZkzPzJiaB?= NVnkSJE3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIx{KGGodHXyJFczKGi{czDifUBHVUODIH3leIhw\CxiSVO1NF0xNjFizszN NVXkOYczOTl6OUS3N|M>
MCF7 cells MX;DfZRwfG:6aXPpeJkh[XO|YYm= NXv1[YhiPzJiaB?= MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyPUSuO{DPxE1? M2\lR|IzOzF4MU[4
human T47D cells NYX5bHFTS3m2b4TvfIlkcXS7IHHzd4F6 M3T0c|czKGh? MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDD[YxtXGm2ZYKtS4xwKGG|c3H5MEBKSzVyPUCuOlYh|ryP NX62S|dEOjJ|MU[xOlg>
SK-BR-3 cells Mnf2R5l1d3SxeHnjbZR6KGG|c3H5 MXW3NkBp NVXVZ|lYS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvQmKtN{Bk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygTWM2OD1zLkC4JO69VQ>? M2LnVFIzOzF4MU[4
Hs578Bst cells M3XHTWN6fG:2b4jpZ4l1gSCjc4PhfS=> MYO3NkBp NGrZb3FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJezV5OFLzeEBk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{Bie3OjeTygTWM2OD1yLkC0JO69VQ>? NUKxVnFFOjJ|MU[xOlg>
human Hs578Bst cells NH3tXVVEgXSxdH;4bYNqfHliYYPzZZk> MUe0NEB2VQ>? MoLHO|IhcA>? M13NRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh{PTd6QoP0JINmdGy|IHH0JFQxKHWPIHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIF{e2G7IILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;sMEBKSzVyPUS3Mlch|ryP NInZOIYzOjNzNkG2PC=>
SK-MEL-28 cells MVXDfZRwfG:6aXPpeJkh[XO|YYm= M1zUTVMh\GG7cx?= NGLzOYFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2PRVytNlgh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1yLkK2OkDPxE1? Mlz6NlM4ODZyMEW=
human U373 cells MmPJR5l1d3SxeHnjbZR6KGG|c3H5 MVWzJIRigXN? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVN|c{KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xOFYh|pyv MWKyN|cxPjByNR?=
human A549 cells M2HrPWN6fG:2b4jpZ4l1gSCjc4PhfS=> NIXNPXc{KGSjeYO= M4DVbmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlA2KM7:TR?= M3\BelI{PzB4MEC1
human PC3 cells M4LQbWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmHmN{Bl[Xm| MU\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1yLkC3OUDPxE1? NXHRWZpTOjN5ME[wNFU>
MCF7 cells MnG2R5l1d3SxeHnjbZR6KGG|c3H5 NFnldIo{KGSjeYO= NUSxW5hkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvPDJ4IN88US=> M1HaPFI{PzB4MEC1
Hs683 cells MnvHR5l1d3SxeHnjbZR6KGG|c3H5 MWqzJIRigXN? NH7sSXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJezZ6MzDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODRizszN Mn3HNlM4ODZyMEW=
CHO cells NFLINFdHfW6ldHnvckBie3OjeR?= NUPtWXB5eEmFNUCgeoFtfWW|IH\vdkB{d2SrdX2g[ox2d3Knc3PlbY4hMDFyIIXNLUB2eHSja3WgbY4hV0GWUEHCNU11emGwc3\lZ5Rm\CCFSF:gZ4VtdHNuIFnDOVA:Oy5{M{W5OEDPxE1? NWnSRW06OjN3N{G0NVU>
rat hepatocytes M360d2Z2dmO2aX;uJIF{e2G7 MVnEdpVoKHWydHHr[UBqdiC{YYSgbIVx[XSxY4n0[ZMtKEuvPUCuNVgh|ryP M4\kPVIxQTJ4NkG5
human hepatocytes M4jHTWZ2dmO2aX;uJIF{e2G7 NVHEcY1xOC5yNTD0c{AyOCC3TR?= NE\SXZJFenWpIIXweIFs\SCrbjDzZY5lf2mlaD3jeYx1fXKnZDDoeY1idiCqZYDheI9kgXSnczDheEAxNjB3IITvJFExKHWPIHH0JFM4KGSnZ1OgZpkhVWmlaHHlcIl{NU2nboTlckBscW6ndHnjd{9{[2mwdHnscIF1cW:wIIPw[YN1em:|Y3;wfUwhU21;Mj6zPUDPxE1? M4DuelIxQTJ4NkG5
CHO cells MmTRSpVv[3Srb36gZZN{[Xl? MYjwTWM2OCC4YXz1[ZMh\m:{IIPv[Il2dSCobIXvdoV{[2WrbjCoNVAhfU1rIIXweIFs\SCrbjDPRXRROUJ|LYTyZY5{\mWldHXkJGNJVyClZXzsd{whUUN3ME2xN{4yQDJ4IN88US=> M2\kNFI{PTdzNEG1
human HeLa cells NV\XNWlvS3m2b4TvfIlkcXS7IHHzd4F6 NVnsXGdsPDhiaB?= MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mj6yJO69VQ>? NYDt[ldjOjZzMkK3O|I>
RKO-AS45-1 cells M2e5cGN6fG:2b4jpZ4l1gSCjc4PhfS=> NXrxT3FXPDhiaB?= NXS5eZFXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWkuRLVHTOFUuOSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOFIh|ryP NGrHO5ozPjF{Mke3Ni=>
Hs578T cells MnfCR5l1d3SxeHnjbZR6KGG|c3H5 M3PmfFczKGh? M371UmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh{PTd6VDDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUeubzDhd5NigSxiSVO1NF0xNjJ3MTFOwG0> NYrYXW9oOjJ|MU[xOlg>
Drosophila Schneider cells NI\m[I1HfW6ldHnvckBie3OjeR?= NULSfmJpSW62YXfvcol{fCCjY4Tpeol1gSCjdDD0doFve2GldHn2ZZRqd25iZH;tZYlvKG:oIGLPVodidW2jdDCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBFem:|b4DobYxiKFOlaH7lbYRmeiClZXzsd{Bkdy2neIDy[ZN{cW6pIFfhcFQuTE6DIHLpcoRqdmdiZH;tZYlvNCCLQ{WwQVIh|ryP MW[yOFA1ODR6Nh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Src / Src / p-EGFR / EGFR / p-STAT3 / STAT3 ; 

PubMed: 25955608     


Analysis of phosphorylated and non-phosphorylated Src, EGFR and STAT3 in A549 cells in a dose-dependent manner. The cancer cells treated with or without various concentrations of digoxin (50, 100, 250 and 500 nM) or 100 nM dasatinib for 24 h were analyzed by Western blot. 

p-PI3K / PI3K / p-AKT / AKT / p-MEK / MEK / p-ERK / ERK / p-FAK / FAK / p-SAPK/JNK / p-Paxillin / Paxillin / p-p130Cas / p130Cas ; 

PubMed: 25955608     


Western blotting analyses of phosphorylated and non-phosphorylated PI3K, AKT, MEK1/2, ERK, FAK, SAPK/JNK, paxillin and p130Cas in (a) A549 cell lines after a 24-h treatment with 100, 250 or 500 nM digoxin. The solvent control was 0.1% DMSO; GAPDH was used as an internal control. 

Nrf2; 

PubMed: 30735911     


Digoxin inhibited the Nrf2 signaling in SW1990/Gem and Panc-1/Gem cells. (A–B) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells. (C–D) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells at different time points.

25955608 30735911
Growth inhibition assay
Cell viability; 

PubMed: 25955608     


Five lung cancer cell lines, (a) A549, (b) H3255, (c) H1975, (d) PC9 and (e) PC9/gef, were treated with 6 different doses (0, 0.01, 0.05, 0.1, 1 and 5 μM) of digoxin at different time points (0, 24, 48, 72 and 96 h), and cell viability was measured using the PrestoBlue Cell Viability reagent after treatment. Quantitative data are presented as the mean±SD (n = 3); *p<0.05 compared with solvent control (0.1% DMSO) at the corresponding time point.

25955608

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (128.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 780.94
Formula

C41H64O14

CAS No. 20830-75-5
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03559868 Not yet recruiting Drug: Digoxin|Other: Placebo Inflammatory Response Yale University August 20 2019 Phase 1
NCT03877965 Enrolling by invitation Drug: Digoxin Congenital Heart Disease Christoph P Hornik MD MPH|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|The Emmes Company LLC|Duke University August 5 2019 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID