Digoxin

For research use only.

Catalog No.S4290

4 publications

Digoxin   Chemical Structure

Molecular Weight(MW): 780.94

Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.

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Biological Activity

Description Digoxin is a classical Na,K-ATPase inhibitor, with selectivity for the α2β3 isoform over the common α1β1 isoform, used in the treatment of atrial fibrillation and heart failure.
Targets
α2β3 [1] α2β1 [1] α1β1 [1]
42.8 nM(Ki) 58.7 nM(Ki) 268 nM(Ki)
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
TK10 cells NEK3SnlEgXSxdH;4bYNqfHliYYPzZZk> MmrhOFghcA>? MnXTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWGsyOCClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuNFE1PiEQvF2= MoHUNVY{ODl|MUW=
MCF7 cells NEfvVY1EgXSxdH;4bYNqfHliYYPzZZk> MljYOFghcA>? MmLhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUCuNFI1OSEQvF2= NFLkOWwyPjNyOUOxOS=>
UACC62 cells MWnDfZRwfG:6aXPpeJkh[XO|YYm= M3ntbFQ5KGh? MkTSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWWFESzZ{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NE4xOjl3IN88US=> MXGxOlMxQTNzNR?=
K562 cells MXjDfZRwfG:6aXPpeJkh[XO|YYm= NEm3PVVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGK7IGjUWEBie3OjeTygTWM2OD1yLkK4NkDPxE1? NI\LSZEyPjNyOUOxOS=>
CC20 cells MUHDfZRwfG:6aXPpeJkh[XO|YYm= M3vIZlczKGh? M4OzSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNEOjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGZOS0FibXX0bI9lNCCLQ{WwQVAvOjRizszN MVqxPVg6PDd|Mx?=
HCT116 cells MoPYR5l1d3SxeHnjbZR6KGG|c3H5 NEP4b|k4OiCq NVzqWIFFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCITVPBJI1mfGixZDygTWM2OD1yLkK3JO69VQ>? MVuxPVg6PDd|Mx?=
HT-29 cells Mk\LR5l1d3SxeHnjbZR6KGG|c3H5 M3;iOFczKGh? M1u2ZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFczKGi{czDifUBHVUODIH3leIhw\CxiSVO1NF0yNjRizszN MXixPVg6PDd|Mx?=
human colon cancer cells MX3DfZRwfG:6aXPpeJkh[XO|YYm= M2LUbVczKGh? MonYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZ49td25iY3HuZ4VzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDGUWNCKG2ndHjv[EwhUUN3ME2wMlEh|ryP NUHodWZVOTl6OUS3N|M>
MCF7 cells NFSxeZJEgXSxdH;4bYNqfHliYYPzZZk> M1X5XlczKGh? NGq5SZlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCLQ{WwQVQvPyEQvF2= M{[wdVIzOzF4MU[4
human T47D cells Mn:4R5l1d3SxeHnjbZR6KGG|c3H5 MWi3NkBp M3myOWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHQ1P0RiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGNmdGyWaYTldk1IdG9iYYPzZZktKEmFNUC9NE43PiEQvF2= M3;3W|IzOzF4MU[4
SK-BR-3 cells M{DGPGN6fG:2b4jpZ4l1gSCjc4PhfS=> MUC3NkBp M4K2[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNUKULUOgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFnDOVA:OS5yODFOwG0> NFqxbpgzOjNzNkG2PC=>
Hs578Bst cells MXrDfZRwfG:6aXPpeJkh[XO|YYm= M2DYU|czKGh? MoP6R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJM2PziEc4SgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XluIFnDOVA:OC5yNDFOwG0> MlPxNlI{OTZzNki=
human Hs578Bst cells NFzMfoREgXSxdH;4bYNqfHliYYPzZZk> NEHXSVM1OCC3TR?= MmPVO|IhcA>? MoHIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJM2PziEc4SgZ4VtdHNiYYSgOFAhfU1iYX\0[ZIhPzJiaILzJIJ6KEOnbHzUbZRmei2JbH:gZZN{[XlicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGlEPTB;NEeuO{DPxE1? MnPvNlI{OTZzNki=
SK-MEL-28 cells NFXlUWNEgXSxdH;4bYNqfHliYYPzZZk> M1La[FMh\GG7cx?= MmGzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOLUK4JINmdGy|IHHmeIVzKDNiZHH5d{BjgSCPVGSgZZN{[XluIFnDOVA:OC5{Nk[g{txO NFHNeYozOzdyNkCwOS=>
human U373 cells Mlf2R5l1d3SxeHnjbZR6KGG|c3H5 M1jGPFMh\GG7cx?= MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVN|c{KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6xOFYh|pyv MXeyN|cxPjByNR?=
human A549 cells MUHDfZRwfG:6aXPpeJkh[XO|YYm= NXywNJB2OyCmYYnz M2LZeWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlA2KM7:TR?= NYHPWFNOOjN5ME[wNFU>
human PC3 cells Mm\yR5l1d3SxeHnjbZR6KGG|c3H5 NHXKU2Y{KGSjeYO= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1yLkC3OUDPxE1? MWSyN|cxPjByNR?=
MCF7 cells NWXEWGtDS3m2b4TvfIlkcXS7IHHzd4F6 NEntcYs{KGSjeYO= MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MD60NlYh|ryP MUCyN|cxPjByNR?=
Hs683 cells M1fpXGN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\2THg{KGSjeYO= MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDId|Y5OyClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUCuNFQh|ryP MnfNNlM4ODZyMEW=
CHO cells MoT4SpVv[3Srb36gZZN{[Xl? NE\YUYZxUUN3MDD2ZYx2\XNiZn;yJJNw\Gm3bTDmcJVwemW|Y3XpckApOTBidV2pJJVxfGGtZTDpckBQSVSSMVKxMZRz[W6|ZnXjeIVlKEOKTzDj[YxteyxiSVO1NF0{NjJ|NUm0JO69VQ>? MkTjNlM2PzF2MUW=
rat hepatocytes M2S2TmZ2dmO2aX;uJIF{e2G7 NF\lbo5FenWpIIXweIFs\SCrbjDyZZQhcGWyYYTvZ5l1\XNuIFvtQVAvOThizszN NVHJTo17OjB7Mk[2NVk>
human hepatocytes NFXXPYlHfW6ldHnvckBie3OjeR?= NF76N2cxNjB3IITvJFExKHWP MV;EdpVoKHWydHHr[UBqdiC|YX7ke4lkcC2ldXz0eZJm\CCqdX3hckBp\XCjdH;jfZRmeyCjdDCwMlA2KHSxIEGwJJVOKGG2IEO3JIRm\0NiYomgUYlkcGGnbHnzMW1mdnSnbjDrbY5mfGmlcz;zZ4lvfGmubHH0bY9vKHOyZXP0do9{[2:yeTygT40:Oi5|OTFOwG0> MnTmNlA6OjZ4MUm=
CHO cells MXLGeY5kfGmxbjDhd5NigQ>? M1[3RZBKSzVyII\hcJVmeyCob4Kgd49lcXWvIH\seY9z\XOlZXnuJEgyOCC3TTmgeZB1[WunIHnuJG9CXFBzQkOteJJidnOoZXP0[YQhS0iRIHPlcIx{NCCLQ{WwQVE{NjF6Mk[g{txO MnfJNlM2PzF2MUW=
human HeLa cells M3;KU2N6fG:2b4jpZ4l1gSCjc4PhfS=> NXvFb3o3PDhiaB?= M4jsfGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yMlIh|ryP MXSyOlEzOjd5Mh?=
RKO-AS45-1 cells M1[xU2N6fG:2b4jpZ4l1gSCjc4PhfS=> MYO0PEBp MnrpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVmtQNUGVNEWtNUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwNEKg{txO NYDuWm0{OjZzMkK3O|I>
Hs578T cells NWDyZWJ{S3m2b4TvfIlkcXS7IHHzd4F6 NFrMO|g4OiCq M2L4bmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh{PTd6VDDj[YxteyCjZoTldkA4OiCqcoOgZpkhS2WubGTpeIVzNUeubzDhd5NigSxiSVO1NF0xNjJ3MTFOwG0> MVOyNlMyPjF4OB?=
Drosophila Schneider cells NHz1O5NHfW6ldHnvckBie3OjeR?= Mn7DRY51[WexbnnzeEBi[3Srdnn0fUBifCC2cnHud4FkfGm4YYTpc44h\G:vYXnuJI9nKFKRUnfhcY1ifCBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDEdo9{d3CqaXzhJHNkcG6naXTldkBk\WyuczDjc{1mgHC{ZYPzbY5oKEejbEStSG5CKGKrbnTpcoch\G:vYXnuMEBKSzVyPUKg{txO MkH3NlQxPDB2OE[=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-Src / Src / p-EGFR / EGFR / p-STAT3 / STAT3 ; 

PubMed: 25955608     


Analysis of phosphorylated and non-phosphorylated Src, EGFR and STAT3 in A549 cells in a dose-dependent manner. The cancer cells treated with or without various concentrations of digoxin (50, 100, 250 and 500 nM) or 100 nM dasatinib for 24 h were analyzed by Western blot. 

p-PI3K / PI3K / p-AKT / AKT / p-MEK / MEK / p-ERK / ERK / p-FAK / FAK / p-SAPK/JNK / p-Paxillin / Paxillin / p-p130Cas / p130Cas ; 

PubMed: 25955608     


Western blotting analyses of phosphorylated and non-phosphorylated PI3K, AKT, MEK1/2, ERK, FAK, SAPK/JNK, paxillin and p130Cas in (a) A549 cell lines after a 24-h treatment with 100, 250 or 500 nM digoxin. The solvent control was 0.1% DMSO; GAPDH was used as an internal control. 

Nrf2; 

PubMed: 30735911     


Digoxin inhibited the Nrf2 signaling in SW1990/Gem and Panc-1/Gem cells. (A–B) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells. (C–D) Effects of digoxin on the protein level of Nrf2 in SW1990/Gem and Panc-1/Gem cells at different time points.

25955608 30735911
Growth inhibition assay
Cell viability; 

PubMed: 25955608     


Five lung cancer cell lines, (a) A549, (b) H3255, (c) H1975, (d) PC9 and (e) PC9/gef, were treated with 6 different doses (0, 0.01, 0.05, 0.1, 1 and 5 μM) of digoxin at different time points (0, 24, 48, 72 and 96 h), and cell viability was measured using the PrestoBlue Cell Viability reagent after treatment. Quantitative data are presented as the mean±SD (n = 3); *p<0.05 compared with solvent control (0.1% DMSO) at the corresponding time point.

25955608

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (128.05 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 780.94
Formula

C41H64O14

CAS No. 20830-75-5
Storage powder
in solvent
Synonyms N/A
Smiles CC1OC(CC(O)C1O)OC2C(O)CC(OC2C)OC3C(O)CC(OC3C)OC4CCC5(C)C(CCC6C5CC(O)C7(C)C(CCC67O)C8=CC(=O)OC8)C4

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04322552 Recruiting Drug: Apatinib Mesylate|Drug: Digoxin tablet Advanced Solid Tumor Jiangsu HengRui Medicine Co. Ltd. March 12 2020 Phase 1
NCT04206488 Suspended Drug: JNJ-70033093|Drug: Digoxin Healthy Janssen Research & Development LLC|Bristol-Myers Squibb January 9 2020 Phase 1
NCT03559868 Not yet recruiting Drug: Digoxin|Other: Placebo Inflammatory Response Yale University August 20 2019 Phase 1
NCT03877965 Enrolling by invitation Drug: Digoxin Congenital Heart Disease Christoph P Hornik MD MPH|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|The Emmes Company LLC|Duke University August 5 2019 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID