Name: Colchicine
Brand: Selleck Chemicals
SKU: S2284
Availability: InStock
Rating: 5 (25 reviews)

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Quality Control

Batch: Purity: 99.99%

99.99

Related Targets	CDK HSP PD-1/PD-L1 ROCK Wee1 DNA/RNA Synthesis Ras KRas Aurora Kinase Casein Kinase
Other Microtubule Associated Inhibitors	Nocodazole Patupilone (Epothilone B) CW069 Combretastatin A4 Lexibulin (CYT997) Epothilone A ABT-751 (E7010) Cucurbitacin B TRx0237 (LMTX) mesylate DM1 (Mertansine)

Solubility

In vitro
Batch:

DMSO : 80 mg/mL (200.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 80 mg/mL

Water : 79 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.000mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.400mg/ml (1.00mM)	Taking the 1 mL working solution as an example, add 50 μL of 8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	399.44	Formula	C₂₂H₂₅NO₆	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	64-86-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Colchineos, Colchisol, Colcin,NSC 757			SMILES	CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.
In vivo	Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.
References	[1] https://pubmed.ncbi.nlm.nih.gov/31628188/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-07-08)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06352632	RECRUITING	Stroke	The George Institute	2024-09-26	PHASE3
NCT07232069	RECRUITING	Coronary Artery Disease Risk Factors Multiple; Coronary Artery Disease Progression; Prevention & Control; Premature Atherosclerosis	Duke University	2026-07-21	PHASE3
NCT06930885	RECRUITING	Atherothrombotic Diseases; Atherosclerotic Cardiovascular Disease (ASCVD)	Hospital do Coracao	2025-06-12	PHASE3
NCT05949281	ACTIVE_NOT_RECRUITING	Type 1 Diabetes; Cardiovascular Diseases; Chronic Inflammation	Asger Lund, MD	2023-08-29	PHASE2
NCT06855433	RECRUITING	CPPD - Calcium Pyrophosphate Deposition Disease	Brigham and Women's Hospital	2026-07-20	PHASE2
NCT07349875	RECRUITING	Coronary Artery Disease (CAD)	Nanjing First Hospital, Nanjing Medical University	2026-01-01	PHASE4

Tech Support

Handling Instructions

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Colchicine Microtubule Associated inhibitor

Chemical Structure

Molecular Weight: 399.44