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Ciprofloxacin Topoisomerase inhibitor

Name: Ciprofloxacin
Brand: Selleck Chemicals
SKU: S2027
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S2027

Ciprofloxacin (Bay o 9867,Bay q 3939) is a fluoroquinolone antibiotic, shows broad and potent antibacterial activity, with MIC90 of 0.024-6 μM.

Chemical Structure

Molecular Weight: 331.34

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S202701 0.5MNAOH]3 mg/mL]false]DMSO]Insoluble]false]Water]Insoluble]false Purity: 99.97%

99.97

Related Targets	DNA/RNA Synthesis HDAC PPAR PARP Sirtuin ATM/ATR Casein Kinase eIF MDM2/MDMX DNA-PK DNA alkylator DHFR Telomerase Nucleoside Analog/Antimetabolite Thymidylate Synthase CRISPR/Cas9 RAD51 BMI-1 LIM kinase RNR NUDIX High-mobility Group OGG1 Alkylating Agent MTH1 G-quadruplex SMN FENs WRN Pax
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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description
human erythrocytes	Growth inhibition assay		50 h	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 infected in human erythrocytes assessed as parasite growth inhibition after 50 hrs by SYBR Green1 dye-based fluorescence assay, IC50=33.9 μM
5637 cells	Function assay		12 h	Antibacterial activity against uropathogenic Escherichia coli UTI89 infected in human 5637 cells assessed as decrease in intracellular bacterial level at 2 to 3 ug/ml after 12 hrs by serial dilution method
CCRF-CEM cells	Proliferation assay	50 μM		Antiproliferative activity against human CCRF-CEM cells assessed as cell viability at 50 uM by CellTiter assay relative to DMSO control
Caco-2 cells	Function assay		30 mins	Antibacterial activity against wild type Salmonella enteritidis 5408 harboring gyrA D87Y mutant gene infected in human Caco-2 cells assessed as effect on bacterial cell adherence after 30 mins
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	331.34	Formula	C₁₇H₁₈FN₃O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	85721-33-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Bay o 9867,Bay q 3939			Smiles	C1CC1N2C=C(C(=O)C3=CC(=C(C=C32)N4CCNCC4)F)C(=O)O

Solubility

In vitro
Batch:

0.5MNAOH : 3 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	5%0.1MNaOH 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.15mg/ml (0.45mM)	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clarified 0.1MNaOH stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% 0.1MNaOH 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.15mg/ml (0.45mM)	Taking the 1 mL working solution as an example, add 50 μL of 3 mg/ml clear 0.1MNaOH stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Topoisomerase IV ^[2]
In vitro	Ciprofloxacin is a fluoroquinolone antibiotic, shows MIC90 (minimal inhibitory concentrations for 90%) of between 0.008 and 2 μg/ml for Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae, Neisseria gonorrhoeae, streptococci, Staphylococcus aureus, and Bacteroidesfragilis strains. ^[1] This compound inhibits topoisomerase IV as a primary topoisomerase target and gyrase as a secondary target. ^[2] It shows an AUC (area under the curve) of 24 μg×h/ml^[3]
References	https://pubmed.ncbi.nlm.nih.gov/6222695/ https://pubmed.ncbi.nlm.nih.gov/8891138/ https://pubmed.ncbi.nlm.nih.gov/10049286/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05828550	Not yet recruiting	Patients withInfections Caused by S.Aureus Like Skin Infections Chest Infections Surgical Site Infections and Urinary Tract Infections	Sohag University	May 2023	Not Applicable
NCT05286931	Recruiting	Neisseria Gonorrhoeae Infection	University of Washington	March 3 2022	Not Applicable

Tech Support

Handling Instructions

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