Allylthiourea

Catalog No.S4017

For research use only.

Allylthiourea is a metabolic inhibitor that selective inhibits ammonia oxidation.

Allylthiourea Chemical Structure

CAS No. 109-57-9

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Biological Activity

Description Allylthiourea is a metabolic inhibitor that selective inhibits ammonia oxidation.
In vitro

Allylthiourea selectively inhibits ammonia oxidation at concentrations 8-80 μM. Allylthiourea (1 μM)inhibits ammonia oxidation by 80%. Complete inhibition is observed at an Allylthiourea concentration of 86 μM. [1] The inhibition of Allylthiourea on ammonia oxidation probably acts through chelating the copper of the ammonia monooxygenase active site. [2] Allylthiourea is able to produce soluble methane monooxygenase (sMMO) in the presence of copper. Addition of 25 μM Allylthiourea decreases intracellular copper by 48% in Methylosinus trichosporium OB3b, allowing sMMO production at Cu/biomass ratios normally not permitting sMMO synthesis, which achieves a plateau of 320 μmol naphthol formed per gram dry biomass per hour. [3]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 116.18
Formula

C4H8N2S

CAS No. 109-57-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C=CCNC(=S)N

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