Milrinone
For research use only.
Catalog No.S2484 Synonyms: Win 47203
2 publications

CAS No. 78415-72-2
Milrinone (Win 47203) is a phosphodiesterase 3 (PDE3) inhibitor, used to increase the heart's contractility.
Selleck's Milrinone has been cited by 2 publications
1 Customer Review
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Viability of H9c2 cells incubated with MRN-HSA-NPs (black bars) compared with MRN-Lactate (grey bars) at different MRN concentrations, at (a) 4 hours, (b) 24 hours and (c) 48 hours. The graph shows a representative result of mean±SD (n=3). ****P<0.0001, ***P<0.001, **P<0.01 and *P<0.05 were considered significant based on Sidak’s posthoc analysis.
Mol Pharm, 2017, doi: 10.1021/acs.molpharmaceut.7b00360. Milrinone purchased from Selleck.
Purity & Quality Control
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Biological Activity
Description | Milrinone (Win 47203) is a phosphodiesterase 3 (PDE3) inhibitor, used to increase the heart's contractility. | ||||||
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Targets |
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In vitro |
Milrinone causes a concentration-dependent increase in the cAMP level in rabbit and human platelets with similar potency. Milrinone inhibits human platelet aggregation with a median inhibitory concentration (IC50) of 2 mM.Milrinone concentration-dependently increases left ventricular developed pressure (LVDP) and contractility. Milrinone concentration-dependently increases cAMP in rabbit coronary smooth muscle cells. [1] Milrinone increases intracellular cyclic adenosine monophosphate by inhibiting Type III phosphodiesterase. [2] Milrinone is a potent (IC50 = 0.16-0.90 mM) and selective (100 times peak III relative to peak I) peak III inhibitor. Milrinone significant increases in cAMP content accompany significant vasorelaxation. [3] |
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In vivo | Milrinone inhibits PDE4 in addition to PDE3 activity in the rabbit heart.Milrinone (>10 microM) causes greater elevations in intracellular cAMP and calcium than cilostazol. [4] Milrinone causes similar increases in heart rate, cardiac output, and left ventricular +dP/dt and decreases in end-diastolic pressure and systemic vascular resistance in anaesthetized dogs. [5] Milrinone leads to significant increases in right ventricular function as well as significant improvements in pulmonary vascular resistance, pulmonary blood flow, and left ventricular filling in mongrel dogs underwent pulmonary artery catheterization. [6] |
Protocol
Solubility (25°C)
In vitro | DMSO | 42 mg/mL (198.84 mM) |
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Water | Insoluble | |
Ethanol | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
Molecular Weight | 211.22 |
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Formula | C12H9N3O |
CAS No. | 78415-72-2 |
Storage |
powder in solvent |
Synonyms | Win 47203 |
Smiles | CC1=C(C=C(C(=O)N1)C#N)C2=CC=NC=C2 |
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
Dosage | mg/kg | Average weight of animals | g | Dosing volume per animal | ul | Number of animals | ||||
Step 2: Enter the in vivo formulation () | ||||||||||
% DMSO % % Tween 80 % ddH2O | ||||||||||
CalculateReset |
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: : mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL,)
Method for preparing in vivo formulation:Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80,mix and clarify, next add μL ddH2O,mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Bio Calculators
Molarity Calculator
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Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
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Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Molarity Calculator
Clinical Trial Information
NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
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NCT03945708 | Recruiting | Device: CytoSorb | Infective Endocarditis | Emma Hansson|CytoSorbents Inc|Sahlgrenska University Hospital Sweden | May 15 2019 | Not Applicable |
NCT04391478 | Completed | Drug: Milrinone|Drug: Sildenafil | Persistent Pulmonary Hypertension | Rania Ali El-Farrash|Ain Shams University | January 2015 | Phase 1 |
NCT01940250 | Completed | Drug: Magnesium Sulphate|Drug: Sterile water | Hand Foot and Mouth Disease | Oxford University Clinical Research Unit Vietnam|Hospital for Tropical Diseases Ho Chi Minh City Vietnam|Children''s Hospital Number 1 Ho Chi Minh City Vietnam | April 21 2014 | Phase 2 |
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