Milrinone

Name: Milrinone
Brand: Selleck Chemicals
SKU: S2484
Rating: 5 (2 reviews)

For research use only.

Catalog No.S2484 Synonyms: Win 47203

2 publications

CAS No. 78415-72-2

Milrinone (Win 47203) is a phosphodiesterase 3 (PDE3) inhibitor, used to increase the heart's contractility.

Selleck's Milrinone has been cited by 2 publications

Biochem Biophys Res Commun, 2018, 503(3):1409-1414

PubMed: 30025893 ( click the link to review the publication )

Mol Pharm, 2017, 10.1021/acs.molpharmaceut.7b00360

PubMed: 28837343 ( click the link to review the publication )

1 Customer Review

Viability of H9c2 cells incubated with MRN-HSA-NPs (black bars) compared with MRN-Lactate (grey bars) at different MRN concentrations, at (a) 4 hours, (b) 24 hours and (c) 48 hours. The graph shows a representative result of mean±SD (n=3). ****P<0.0001, ***P<0.001, **P<0.01 and *P<0.05 were considered significant based on Sidak’s posthoc analysis.

Mol Pharm, 2017, doi: 10.1021/acs.molpharmaceut.7b00360. Milrinone purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description

Milrinone (Win 47203) is a phosphodiesterase 3 (PDE3) inhibitor, used to increase the heart's contractility.

Targets

ATPase ^[1]	PDE3 ^[7]	PDE2 ^[7]
	2.1 μM	5.2 μM

In vitro

Milrinone causes a concentration-dependent increase in the cAMP level in rabbit and human platelets with similar potency. Milrinone inhibits human platelet aggregation with a median inhibitory concentration (IC50) of 2 mM.Milrinone concentration-dependently increases left ventricular developed pressure (LVDP) and contractility. Milrinone concentration-dependently increases cAMP in rabbit coronary smooth muscle cells. ^[1] Milrinone increases intracellular cyclic adenosine monophosphate by inhibiting Type III phosphodiesterase. ^[2] Milrinone is a potent (IC50 = 0.16-0.90 mM) and selective (100 times peak III relative to peak I) peak III inhibitor. Milrinone significant increases in cAMP content accompany significant vasorelaxation. ^[3]

In vivo

Milrinone inhibits PDE4 in addition to PDE3 activity in the rabbit heart.Milrinone (>10 microM) causes greater elevations in intracellular cAMP and calcium than cilostazol. ^[4] Milrinone causes similar increases in heart rate, cardiac output, and left ventricular +dP/dt and decreases in end-diastolic pressure and systemic vascular resistance in anaesthetized dogs. ^[5] Milrinone leads to significant increases in right ventricular function as well as significant improvements in pulmonary vascular resistance, pulmonary blood flow, and left ventricular filling in mongrel dogs underwent pulmonary artery catheterization. ^[6]

Protocol

References

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Solubility (25°C)

In vitro	DMSO	42 mg/mL (198.84 mM)
	Water	Insoluble
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Milrinone SDF

Molecular Weight	211.22
Formula	C₁₂H₉N₃O
CAS No.	78415-72-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Win 47203
Smiles	CC1=C(C=C(C(=O)N1)C#N)C2=CC=NC=C2

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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The Serial Dilution Calculator Equation

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Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03945708	Recruiting	Device: CytoSorb	Infective Endocarditis	Emma Hansson\|CytoSorbents Inc\|Sahlgrenska University Hospital Sweden	May 15 2019	Not Applicable
NCT04391478	Completed	Drug: Milrinone\|Drug: Sildenafil	Persistent Pulmonary Hypertension	Rania Ali El-Farrash\|Ain Shams University	January 2015	Phase 1
NCT01940250	Completed	Drug: Magnesium Sulphate\|Drug: Sterile water	Hand Foot and Mouth Disease	Oxford University Clinical Research Unit Vietnam\|Hospital for Tropical Diseases Ho Chi Minh City Vietnam\|Children''s Hospital Number 1 Ho Chi Minh City Vietnam	April 21 2014	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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PDE Signaling Pathway Map

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Milrinone