IBMX

Catalog No.S5836 Synonyms: Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine

For research use only.

IBMX (Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine) is a nonspecific inhibitor of phosphodiesterase (PDE) with IC50 values of 6.5±1.2, 26.3±3.9 and 31.7±5.3 μM for PDE3, 4 and 5 respectively. It may enhance the intracellular cAMP levels and also acts as an adenosine (A1) receptor antagonist.

IBMX Chemical Structure

CAS No. 28822-58-4

Selleck's IBMX has been cited by 7 Publications

Purity & Quality Control

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Biological Activity

Description IBMX (Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine) is a nonspecific inhibitor of phosphodiesterase (PDE) with IC50 values of 6.5±1.2, 26.3±3.9 and 31.7±5.3 μM for PDE3, 4 and 5 respectively. It may enhance the intracellular cAMP levels and also acts as an adenosine (A1) receptor antagonist.
Targets
PDE3 [1] PDE4 [1] PDE5 [1]
6.5 μM 26.3 μM 31.7 μM
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
adipocytes, heart and brain cells Mmm1SpVv[3Srb36gZZN{[Xl? MkXoRYZncW6rdImgZ49ve3SjboSg[o9zKGmwaHnibZRqd25ib3[gRVEhemWlZYD0c5Ih[2:wdILvcEBw\iCjZHXufYxifGViY4njcIF{\SCrbjDh[Ilxd2O7dHXzMEBp\WG{dDDhcoQh[nKjaX6gZ4VtdHNuIFHm[olvcXS7IHPvcpN1[W62PUNOwG0> M4Dk[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzZ{N{m4OFAoRjZ{N{m4OFA9N2F-
adipocytes, heart and brain cells NVjUTYdWTnWwY4Tpc44h[XO|YYm= MXzB[oZqdmm2eTDjc45{fGGwdDDmc5IhSTJicnXj[ZB1d3JiY3;ueJJwdCCxZjDh[IVvgWyjdHWgZ5lkdGG|ZTDpckBi\Gmyb3P5eIV{NCCqZXHyeEBidmRiYoLhbY4h[2WubIOsJGFn\mmwaYT5JINwdnO2YX70QVXPxE1? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek83Ojd7OESwK|43Ojd7OESwQE9iRg>?
3T3L1 M4nnfGZ2dmO2aX;uJIF{e2G7 MXKwMlI2KG2P MVW0PEBpenN? NFvCO49UfGmvdXzheIlwdiCxZjDsbZBwdHm|aYOgbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XRiMD6yOUBuVSCjZoTldkA1QCCqcoOgZpkh\2y7Y3Xyc4whemWuZXHz[UBie3OjeR?= NV;tfZFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PVc5OjBpPkK0OVk4QDJyPD;hQi=>
BL21(DE3) pLysS NYXpbItlTnWwY4Tpc44h[XO|YYm= NYfX[ItCUW6qaXLpeIlwdiCxZjDoeY1idiCKaYOteIFo\2WmIGDESVNDKGOjdHHsfZRq[yCmb33hbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUhFTTNrIIDMfZNUKGOnbHzzMEBKSzVyPUCuNlQz|ryP M3\jR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUC4NFI2Lz5{NkmwPFAzPTxxYU6=
Sf21 M1rHUmZ2dmO2aX;uJIF{e2G7 MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGMufGW{bXnuZYwhUGm|Nj30ZYdo\WRiUFTFNmEyKCh{MUWgeI8hQTByIILld4llfWW|KTDlfJBz\XO|ZXSgbY4he2Z{MTDj[YxteyxiSVO1NF01|ryP M2rEZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUC4NFI2Lz5{NkmwPFAzPTxxYU6=
BL21-CodonPlus MojMSpVv[3Srb36gZZN{[Xl? MVPJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEirc{[teIFo\2WmIGDESVVCKCh3M{WgeI8hPzh4IILld4llfWW|KT;QSGU3SyCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyNUOxZH;uVIx2eyClZXzsd{B2e2mwZzDbN2he[0ePUDDhd{B{fWK|dILheIUtKEurPUiuOe69VQ>? NYXC[oVqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5NFgxOjVpPkK2PVA5ODJ3PD;hQi=>
Sf9 NHT0PZpHfW6ldHnvckBie3OjeR?= NVr0SYdvOTBibXnudy=> Mm\pTY5pcWKrdHnvckBw\iCqdX3hckBIW1RvdHHn[4VlKFCGRUTCJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3czDpcoZm[3SnZDDT[lkhcW6|ZXP0JINmdGy|IIXzbY5oKGODTWCgZZMhe3Wkc4TyZZRmKGGodHXyJFExKG2rboOgZpkhWESHLVfsc{BRcG:|cHjv[Ilme3SncnHz[UBCe3OjeTygTWM2OD1{OD6y{txO NHHNc4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSwO|k3PSd-Mkm0NFc6PjV:L3G+

Protocol (from reference)

Animal Research:

[2]

  • Animal Models: Sprague-Dawley rats
  • Dosages: 0.5, 5.0, or 10.0 mg/5 μl
  • Administration: i.c.v.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 222.24
Formula

C10H14N4O2

CAS No. 28822-58-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)CN1C2=C(C(=O)N(C1=O)C)NC=N2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02807415 Completed Drug: Lumacaftor plus Ivacaftor Cystic Fibrosis Hannover Medical School|Heidelberg University|University of Giessen June 1 2016 --
NCT03652090 Completed Procedure: cell sampling Cystic Fibrosis Institut National de la Santé Et de la Recherche Médicale France|ABCF2 September 1 2010 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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