Rolipram (ZK-62711)
For research use only.
Catalog No.S1430 Synonyms: SB 95952
13 publications
CAS No. 61413-54-5
The PDE4 selective inhibitor, Rolipram (ZK-62711, SB 95952), inhibited immunopurified PDE4B and PDE4D activities similarly, with IC50 values of approx. 130 nM and 240 nM respectively; an anti-inflammatory agent.
5 Customer Reviews
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D, average percentage difference of cell growth by PDE4D inhibitors between STK11 mutant and wild-type cell lines. The sensitivity on STK11mt cell lines represents the difference of average percentage growth between STK11-mutant (NCI-H1993 and NCI-H1395) and wild-type cell lines (NCI-H82 and NCI-H524). Average percentage growth of each cell line by a PDE4D inhibitor was based on the DMSO control. Cells were treated with a 20 μmol/L of PDE4D inhibitors for 3, 7, and 10 days incubation, respectively. The cell viability was determined using CellTiter-Blue fluorescence. *, P < 0.01 and **, P < 0.05 in the difference between STK11-mutant and wild-type cell lines. All cells used in the experiment were cultured in normal conditioned RPMI medium with 10% FBS and 1% penicillin/streptavidin.
Mol Cancer Ther, 2014, 13(10):2463-73.. Rolipram (ZK-62711) purchased from Selleck.
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Rolipram attenuated neuronal apoptosis in ipsilateral cortex after SAH. (A) Representative transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL)/NeuN photomicrographs of the ipsilateral cortex in different groups (scale bar=50 μm). Fluorescence colors: DAPI, blue; TUNEL, green；and NeuN, red. (B) Quantification of the TUNEL/NeuN positive cells in the ipsilateral cortex in different groups. n=4. Data were presented as mean ± SD. *P < .05 versus sham group; #P < .05 versus SAH+vehicle group.
Biomed Pharmacother, 2018, 99:947-955. Rolipram (ZK-62711) purchased from Selleck.
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Panel C shows the effect of rolipram against H2O2 toxicity and hypoxia in PC12, SH-SY5Y, and NT2 cell lines (*P < .5 vs group without rolipram treatment). shRNA ¼ short hairpin RNA.
J Sex Med, 2016, 13(12):1834-1843. Rolipram (ZK-62711) purchased from Selleck.
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Representative microphotographs showed the co-localization of NeuN (red) with TUNEL (green)-positive cells in the ipsilateral cortex at 24 h after SAH.
Neurochem Res, 2018, 43(4):785-795. Rolipram (ZK-62711) purchased from Selleck.
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PDE inhibitor increases SIRT1 protein and mRNA expression and p-AMPK protein expression. Resveratrol and rolipram, a PDE inhibitor, significantly increased SIRT1 mRNA levels (A) and protein expression (B). (C) Western blot analysis showed that both resveratrol and rolipram increased AMPK phosphorylation levels. Results are expressed as the mean ± S.E.M. (*P < 0.05, **P < 0.01, n = 4).
Brain Res Bull, 2016, 121:255-62. Rolipram (ZK-62711) purchased from Selleck.
Purity & Quality Control
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Biological Activity
| Description | The PDE4 selective inhibitor, Rolipram (ZK-62711, SB 95952), inhibited immunopurified PDE4B and PDE4D activities similarly, with IC50 values of approx. 130 nM and 240 nM respectively; an anti-inflammatory agent. | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Cell Data |
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Protocol
Solubility (25°C)
| In vitro | DMSO | 55 mg/mL (199.75 mM) |
|---|---|---|
| Water | Insoluble | |
| Ethanol | '55 mg/mL warmed |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 275.34 |
|---|---|
| Formula | C16H21NO3 |
| CAS No. | 61413-54-5 |
| Storage |
powder in solvent |
| Synonyms | SB 95952 |
| Smiles | COC1=C(C=C(C=C1)C2CC(=O)NC2)OC3CCCC3 |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Bio Calculators
Molarity Calculator
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Dilution Calculator
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|---|
| NCT01215552 | Terminated | Drug: HT-0712 | Healthy Elderly Volunteers | Dart NeuroScience LLC | September 2010 | Phase 1 |
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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