Rolipram (ZK-62711)

For research use only.

Catalog No.S1430 Synonyms: SB 95952

15 publications

Rolipram (ZK-62711) Chemical Structure

CAS No. 61413-54-5

The PDE4 selective inhibitor, Rolipram (ZK-62711, SB 95952), inhibited immunopurified PDE4B and PDE4D activities similarly, with IC50 values of approx. 130 nM and 240 nM respectively; an anti-inflammatory agent.

Selleck's Rolipram (ZK-62711) has been cited by 15 publications

5 Customer Reviews

  • D, average percentage difference of cell growth by PDE4D inhibitors between STK11 mutant and wild-type cell lines. The sensitivity on STK11mt cell lines represents the difference of average percentage growth between STK11-mutant (NCI-H1993 and NCI-H1395) and wild-type cell lines (NCI-H82 and NCI-H524). Average percentage growth of each cell line by a PDE4D inhibitor was based on the DMSO control. Cells were treated with a 20 μmol/L of PDE4D inhibitors for 3, 7, and 10 days incubation, respectively. The cell viability was determined using CellTiter-Blue fluorescence. *, P < 0.01 and **, P < 0.05 in the difference between STK11-mutant and wild-type cell lines. All cells used in the experiment were cultured in normal conditioned RPMI medium with 10% FBS and 1% penicillin/streptavidin.

    Mol Cancer Ther, 2014, 13(10):2463-73.. Rolipram (ZK-62711) purchased from Selleck.

  • Rolipram attenuated neuronal apoptosis in ipsilateral cortex after SAH. (A) Representative transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL)/NeuN photomicrographs of the ipsilateral cortex in different groups (scale bar=50 μm). Fluorescence colors: DAPI, blue; TUNEL, green;and NeuN, red. (B) Quantification of the TUNEL/NeuN positive cells in the ipsilateral cortex in different groups. n=4. Data were presented as mean ± SD. *P < .05 versus sham group; #P < .05 versus SAH+vehicle group.

    Biomed Pharmacother, 2018, 99:947-955. Rolipram (ZK-62711) purchased from Selleck.

  • Panel C shows the effect of rolipram against H2O2 toxicity and hypoxia in PC12, SH-SY5Y, and NT2 cell lines (*P < .5 vs group without rolipram treatment). shRNA ¼ short hairpin RNA.

    J Sex Med, 2016, 13(12):1834-1843. Rolipram (ZK-62711) purchased from Selleck.

  • Representative microphotographs showed the co-localization of NeuN (red) with TUNEL (green)-positive cells in the ipsilateral cortex at 24 h after SAH.

    Neurochem Res, 2018, 43(4):785-795. Rolipram (ZK-62711) purchased from Selleck.

  • PDE inhibitor increases SIRT1 protein and mRNA expression and p-AMPK protein expression. Resveratrol and rolipram, a PDE inhibitor, significantly increased SIRT1 mRNA levels (A) and protein expression (B). (C) Western blot analysis showed that both resveratrol and rolipram increased AMPK phosphorylation levels. Results are expressed as the mean ± S.E.M. (*P < 0.05, **P < 0.01, n = 4).

    Brain Res Bull, 2016, 121:255-62. Rolipram (ZK-62711) purchased from Selleck.

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Biological Activity

Description The PDE4 selective inhibitor, Rolipram (ZK-62711, SB 95952), inhibited immunopurified PDE4B and PDE4D activities similarly, with IC50 values of approx. 130 nM and 240 nM respectively; an anti-inflammatory agent.
Targets
PDE4B [1]
(Cell-free assay)
PDE4D [1]
(Cell-free assay)
130 nM 240 nM
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf9 cells Mkm0SpVv[3Srb36gZZN{[Xl? NXO5SmNmUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDQSGU1SSCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzMEBKSzVyPUSgcm0> M2fmWVEyODV{N{i1
human PBMC M33Z[GZ2dmO2aX;uJIF{e2G7 M{W3[WlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5H[WyyaHGgdJJw\HWldHnvckBqdiCqdX3hckBRSk2FLDDJR|UxRTBwME[g{txO NUTKT2JkOTlyNEmzOFk>
COS7 cells MXLGeY5kfGmxbjDhd5NigQ>? MYfJcohq[mm2aX;uJI9nKFCGRUTCNkBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczDhd5Nme3OnZDDhd{BkSU2SIHj5[JJwdHm|aYOsJGlEPTB;MD6xNFUh|ryP NGPoeVgyQDJ{MkC4PC=>
HEK293 cells M3SzTGZ2dmO2aX;uJIF{e2G7 NXjYc|dWUW6qaXLpeIlwdiCxZjDQSGU1KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[2:neIDy[ZN{cW6pIFetdJJwfGWrbjDjc5VxdGWmIILlZ4VxfG:{IHHu[EBkgWOuaXOgcpVkdGWxdHnk[UBo[XSnZDDpc44h[2ijbn7lcEBjgSCobIXvdoV{[2WwY3WgZZN{[XluIFXDOVA:OC5zM{G1JO69VQ>? NUDoXGY1OTl2NkS4PFY>
rat UMR106 cells NYLuZo9yTnWwY4Tpc44h[XO|YYm= MofBTY5pcWKrdHnvckBw\iCSRFWgbY4hemG2IGXNVlExPiClZXzsd{Bkdy2neIDy[ZN{cW6pIFPSSU1tfWNiYYPz[ZN{\WRiYYOgVHRJNWmwZIXj[YQh[0GPUDDhZ4N2dXWuYYTpc44h[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuOkDPxE1? MUKyNlA{ODB|MB?=
human U937 cells MorKSpVv[3Srb36gZZN{[Xl? M3Sxc2lvcGmkaYTpc44hd2ZiUFTFOEBqdiCqdX3hckBWQTN5IHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBw\iCdM1jdZYRmdm:|aX7lJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGlEPTB;MD64OkDPxE1? M1vBcFE6OzB|Mkmw

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 55 mg/mL (199.75 mM)
Water Insoluble
Ethanol '55 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 275.34
Formula

C16H21NO3

CAS No. 61413-54-5
Storage powder
in solvent
Synonyms SB 95952
Smiles COC1=C(C=C(C=C1)C2CC(=O)NC2)OC3CCCC3

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01215552 Terminated Drug: HT-0712 Healthy Elderly Volunteers Dart NeuroScience LLC September 2010 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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PDE Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID