Choose Your Country or Region

Rolipram

Name: Rolipram
Brand: Selleck Chemicals
SKU: S1430
Rating: 5 (17 reviews)

Synonyms: ZK-62711, SB 95952

Catalog No.S1430 Purity:99.95%

The PDE4 selective inhibitor, Rolipram, inhibited immunopurified PDE4B and PDE4D activities similarly, with IC50 values of approx. 130 nM and 240 nM respectively; an anti-inflammatory agent.

Rolipram Chemical Structure

CAS No. 61413-54-5

Purity & Quality Control

Batch: Purity: 99.95%

99.95

Rolipram Related Products

Related Targets	PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE10A PDE9 PDE4B	Click to Expand
Related Products	IBMX Icariin Mardepodect (PF-2545920) Cilomilast Deltarasin PF-8380 Enpp-1-IN-1 Ibudilast GSK256066 Ziritaxestat (GLPG1690) TAK-063 Doxofylline Diosgenin BRL-50481 S-(+)-Rolipram	Click to Expand
Related Compound Libraries	Metabolism Compound Library Anti-cancer Metabolism Compound Library Glutamine Metabolism Compound Library Carbohydrate Metabolism Compound Library Lipid Metabolism Compound Library	Click to Expand

Signaling Pathway

PDE Signaling Pathway

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
Sf9 cells	Function assay	Inhibition of recombinant human PDE4A expressed in Sf9 cells, IC50=4 nM	11052785
human PBMC	Function assay	Inhibition of LPS-induced TNFalpha production in human PBMC, IC50=0.06 μM	19049349
COS7 cells	Function assay	Inhibition of PDE4B2 expressed in COS7 cells assessed as cAMP hydrolysis, IC50=0.105 μM	18222088
HEK293 cells	Function assay	Inhibition of PDE4 expressed in HEK293 cells coexpressing G-protein coupled receptor and cyclic nucleotide gated ion channel by fluorescence assay, EC50=0.1315 μM	19464886
rat UMR106 cells	Function assay	Inhibition of PDE in rat UMR106 cells co-expressing CRE-luc assessed as PTH-induced cAMP accumulation by luciferase reporter gene assay, IC50=0.6 μM	22030030
human U937 cells	Function assay	Inhibition of PDE4 in human U937 cells assessed as accumulation of [3H]adenosine by scintillation counting, IC50=0.86 μM	19303290
Click to View More Cell Line Experimental Data

Biological Activity

Description

Targets

PDE4B ^[1] (Cell-free assay)	PDE4D ^[1] (Cell-free assay)
130 nM	240 nM

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT01215552	Terminated	Healthy Elderly Volunteers	Dart NeuroScience LLC	September 2010	Phase 1

References

[1] MacKenzie SJ, et al. Biochem J. 2000, 347(Pt 2):571-8.

Chemical Information & Solubility

Molecular Weight	275.34	Formula	C₁₆H₂₁NO₃
CAS No.	61413-54-5	SDF	Download Rolipram SDF
Smiles	COC1=C(C=C(C=C1)C2CC(=O)NC2)OC3CCCC3
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 55 mg/mL ( (199.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 55 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.300mg/ml (8.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.300mg/ml (8.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):