Home DNA Damage/DNA Repair Topoisomerase inhibitor Irinotecan (CPT-11) HCl Trihydrate

Irinotecan (CPT-11) HCl Trihydrate

Catalog No.S2217

For research use only.

Irinotecan (CPT-11) prevents DNA from unwinding by inhibition of topoisomerase 1.

Irinotecan (CPT-11) HCl Trihydrate Chemical Structure

CAS No. 136572-09-3

Selleck's Irinotecan (CPT-11) HCl Trihydrate has been cited by 71 publications

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Related Compound Libraries

Other Topoisomerase Products

Download Inhibitor Catalog

Download Inhibitor Catalog
Topoisomerase Signaling Pathways

Topoisomerase Signaling Pathway Map

Biological Activity

Description Irinotecan (CPT-11) prevents DNA from unwinding by inhibition of topoisomerase 1.
Features Irinotecan is a prodrug that is used to treat metastatic colorectal cancer.
Targets
Topo I [1]
In vitro

Irinotecan is activated to SN-38 by carboxylesterases to become able to interact with its target, topoisomerase I. Irinotecan induces similar amounts of cleavable complexes at its IC50 in LoVo cells and HT-29 cell lines. SN-38 induces a concentration-dependent formation of cleavable complexes, which is not significantly different in LoVo cells and HT-29 cell lines. Cell accumulation of Irinotecan is markedly different, reaching consistently higher levels in HT-29 cells than in LoVo cells. [1] The lactone E-ring of Irinotecan and SN-38 hydrolyses reversibly in aqueous solutions, and the interconversion between the lactone and carboxylate forms is dependent on pH and temperature. Liver is primarily responsible for the activation of Irinotecan to SN-38. At equal concentrations of Irinotecan and SN-38 glucuronide, the rate of beta-glucuronidase-mediated SN-38 production is higher than that formed from Irinotecan in both tumour and normal tissue. [2] Irinotecan is also converted to SN-38 in intestines, plasma and tumor tissues. [3] Irinotecan is significantly more active in SCLC than in NSCLC cell lines, whereas no significant difference between histological types is observed with SN-38. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RPMI8402 M2D0PWN6fG:2b4jpZ4l1gSCjc4PhfS=> M{izT|Qh\GG7cx?= NYnPOmEyS3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gcJlueGixYnzhd5QhfHWvb4KgZ4VtdCCuaX7lJHJRVUl6NECyJIFnfGW{IESg[IF6eyCxZjD0doVifG2nboSuJGlEPTB;MD61O{DPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjd2N{e5PEc,OTJ5NEe3PVg9N2F-
Hep3B NW\xZYpRSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYT3VpJXPzJiaILz NUHycIlMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[ZA{SiClZXzsd{Bi\nSncjC3NkBpenNiYomgXHRVKGG|c3H5MEBIUTVyIE2gOE44OyEQvF2u MkDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5OU[5OVYoRjF7N{m2PVU3RC:jPh?=
HepG2 MYjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MkfpO|IhcHK| MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC[VGSgZZN{[XluIFfJOVAhRSB3Lkm0JO69VS5? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd7Nkm1Okc,OTl5OU[5OVY9N2F-
NCI60 M4r1[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3vaV2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG5EUTZyIHPlcIx{KGK7IGPCVkBie3OjeTygS2k2OCB;IEG0MlEzPTRizszNMi=> NV\lc5NLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwN|U{OzRpPkKxNFM2OzN2PD;hQi=>
Hep3B NXvTUXpyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkW3O|IhcHK| M2nxNWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmeDOEIHPlcIx{KGGodHXyJFczKGi{czDifUBZXFRiYYPzZZktKEeLNUCgQUA1Njd|IN88UU4> NETubVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC3PVI2PCd-MkKwO|kzPTR:L3G+
HepG2 MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVO4cZY{PzJiaILz M3K1Nmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFczKGi{czDifUBZXFRiYYPzZZktKEeLNUCgQUA2Njl2IN88UU4> Mmq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{myOVQoRjJ{MEe5NlU1RC:jPh?=
MDA-MB-231 M2\Jb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUO3NkBpenN? NGXZSXhIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTBiPTC5MlE4KM7:TT6= M4XBd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEe5NlU1Lz5{MkC3PVI2PDxxYU6=
A549 M1jIVWN6fG:2b4jpZ4l1gSCjc4PhfS=> M3\SUVczKGi{cx?= M{TTO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gOUDPxE1w MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh4N{CxPUc,OjJ6NkewNVk9N2F-
HCT116 MYLDfZRwfG:6aXPpeJkh[XO|YYm= MV[3NkBpenN? NY\TNVNbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy|IHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDdwMTFOwG0v NGnLVXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki2O|AyQSd-MkK4OlcxOTl:L3G+
MDA-MB-435 M3\DdGN6fG:2b4jpZ4l1gSCjc4PhfS=> NX;uUnpRPzJiaILz MmO0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFfJOVAhRSBzMj6yJO69VS5? MkfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NkewNVkoRjJ{OE[3NFE6RC:jPh?=
K562 NFvqNnBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mn;JO|IhcHK| NEDsV3VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiN{KgbJJ{NCCJSUWwJF0hOS57IN88UU4> NXr2elJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0NlAyPzVpPkK1OFIxOTd3PD;hQi=>
A549 M2TQU2FvfGm2dX3vdkBie3OjeR?= MVG0JIhzew>? NEe1ZWRCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IESgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IE[uOVI5KM7:TT6= M1HN[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkC3O|Q5Lz5zOEKwO|c1QDxxYU6=
LOVO NGG5NYxCdnSrdIXtc5Ih[XO|YYm= NVTZfI5bPCCqcoO= NVXoNIt6SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUG9XVyClZXzsd{Bi\nSncjC0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC5MlAyPSEQvF2u NEOxe449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKwO|c1QCd-MUiyNFc4PDh:L3G+
MCF7 NIqycIZCdnSrdIXtc5Ih[XO|YYm= MV:0JIhzew>? MYHBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDF5LkSwN{DPxE1w M1e0NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkC3O|Q5Lz5zOEKwO|c1QDxxYU6=
LS174T NE\lcIVEgXSxdH;4bYNqfHliYYPzZZk> M2jzeFk3KGi{cx?= NHnWV3JEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNWzF5NGSgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwMU[g{txONg>? NFPVOZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWxN|k4Pid-MUi1NVM6PzZ:L3G+
KB3-1 MmPlR5l1d3SxeHnjbZR6KGG|c3H5 MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRlMuOSClZXzsd{BjgSCPVGSgcYV1cG:mLDDJR|UxKD1iMD62PEDPxE1w MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDd5MUmzNEc,OTh5N{G5N|A9N2F-
KBH5.0 M1fGZmN6fG:2b4jpZ4l1gSCjc4PhfS=> NXK4O2FvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0KKNT6wJINmdGy|IHL5JG1VXCCvZYToc4QtKEmFNUCgQUA4NjRizszNMi=> M1eyOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6N{exPVMxLz5zOEe3NVk{ODxxYU6=
KBV1 MlvRR5l1d3SxeHnjbZR6KGG|c3H5 M2KxWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDXjFiY3XscJMh[nliTWTUJI1mfGixZDygTWM2OCB;IESwJO69VS5? NGW4eJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe3NVk{OCd-MUi3O|E6OzB:L3G+
MDA-MB-435 NITMcYdEgXSxdH;4bYNqfHliYYPzZZk> MoG1O|IhcHK| MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkG0JO69VS5? NEDZdXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEO3NVE5Oyd-MkCzO|EyQDN:L3G+
A549 MYXDfZRwfG:6aXPpeJkh[XO|YYm= M2flS|czKGi{cx?= NUHTO3UzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hPC54MTFOwG0v NWXMclVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzO|EyQDNpPkKwN|cyOTh|PD;hQi=>
LoVo MofPR5l1d3SxeHnjbZR6KGG|c3H5 MXW3NkBpenN? M4K5TmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxwXm9iY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFQvQTlizszNMi=> MnXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|N{GxPFMoRjJyM{exNVg{RC:jPh?=
A549 NH;2dZdEgXSxdH;4bYNqfHliYYPzZZk> NVrXdFY3OyCmYYnz M4XwTmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFYvPCEQvF2u NGnYV4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm0NlQ6OCd-MkC5OFI1QTB:L3G+
LoVo NFnQWZFEgXSxdH;4bYNqfHliYYPzZZk> MorUN{Bl[Xm| MoHxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUI9XdyClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gPE45KM7:TT6= M1q5WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUSyOFkxLz5{MEm0NlQ6ODxxYU6=
MDA-MB-435 NX\MNpRuS3m2b4TvfIlkcXS7IHHzd4F6 MlfuN{Bl[Xm| NXTKfJlOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNVch|ryPLh?= NYWzS4VYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC5OFI1QTBpPkKwPVQzPDlyPD;hQi=>
A375 MXPDfZRwfG:6aXPpeJkh[XO|YYm= MVm3NkBpenN? M1;yXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE{PzViY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyIE2gNE4xODRizszNMi=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2MU[3OEc,OjF|NEG2O|Q9N2F-
PC3 NH7sTZhEgXSxdH;4bYNqfHliYYPzZZk> NIDjOpY4OiCqcoO= MmDuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGM{KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygTWM2OCB;IECuNFA1KM7:TT6= MmniQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
LNCAP NUfodVl3S3m2b4TvfIlkcXS7IHHzd4F6 M2rhN|czKGi{cx?= M1XXS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODB7IN88UU4> MnLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
MESSA M2T0fmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{jlPFczKGi{cx?= NWna[YJ2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUWVU1GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwJF0hOC5yMTFOwG0v MoL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
H460 NYO1cGNzS3m2b4TvfIlkcXS7IHHzd4F6 MWi3NkBpenN? NFH0OllEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODF3IN88UU4> MmLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
H69 NIfMd2VEgXSxdH;4bYNqfHliYYPzZZk> MWi3NkBpenN? M3Ts[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGg3QSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTBiPTCwMlAzOiEQvF2u NETwdoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0NVY4PCd-MkGzOFE3PzR:L3G+
MES-SA/Dx5 NYTtRmJIS3m2b4TvfIlkcXS7IHHzd4F6 NEHSfW84OiCqcoO= NYnKTWplS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUWVLWPBM2R5PSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDNSHIyKGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3MDC9JFAvODJ{IN88UU4> MonqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
H460 MmrVR5l1d3SxeHnjbZR6KGG|c3H5 NWXJdYdrPzJiaILz MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTDpckBxemW|ZX7j[UBw\iB2MDDt[{9udCCKU1GsJGlEPTBiPTCwMlAzPyEQvF2u MlLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEG2O|QoRjJzM{SxOlc1RC:jPh?=
IGROV1 M{myTGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVHTNmt{PzJiaILz MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDJS3JQXjFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MEBKSzVyIE2gNE4xOyEQvF2u NIPGUFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0NVY4PCd-MkGzOFE3PzR:L3G+
SK-MEL-2 NHzLZYdEgXSxdH;4bYNqfHliYYPzZZk> NITQe5I4OiCqcoO= M4jkTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HTD2yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NEA:KDBwMTFOwG0v NGLLUI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0NVY4PCd-MkGzOFE3PzR:L3G+
MALME-3M NWHSSFJrS3m2b4TvfIlkcXS7IHHzd4F6 MYm3NkBpenN? NWG5Z5lbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUGOTVWtN20h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxKD1iMD6yJO69VS5? M3PyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxOlc1Lz5{MUO0NVY4PDxxYU6=
DU145 M2TDUmN6fG:2b4jpZ4l1gSCjc4PhfS=> MXu3NkBpenN? NH74TZREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUCgQUAxNjJizszNMi=> M4XZclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxOlc1Lz5{MUO0NVY4PDxxYU6=
HT-29 NFnScYdEgXSxdH;4bYNqfHliYYPzZZk> M2GzWlczKGi{cx?= NWnpdG1ES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwJF0hOC5{MjFOwG0v M1XtZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxOlc1Lz5{MUO0NVY4PDxxYU6=
H69AR M{OxZWN6fG:2b4jpZ4l1gSCjc4PhfS=> NUTy[I1ZPzJiaILz NUD3VY9HS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDZ7QWKgZ4VtdHNib4\ldoV5eHKnc4PpcochVUSUMTDh[pRmeiB5MjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFnDOVAhRSByLkK1JO69VS5? M3TGfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{SxOlc1Lz5{MUO0NVY4PDxxYU6=
DU145 NGHzNGhEgXSxdH;4bYNqfHliYYPzZZk> NUjTN5NYS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYomgV3JDKGG|c3H5MEBKSzVyIE2gPU4{KM7:TT6= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzFyMki5N{c,OjNzMEK4PVM9N2F-
A549 NETwOVZEgXSxdH;4bYNqfHliYYPzZZk> NIP0OJhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGK7IGPSRkBie3OjeTygTWM2OCB;IEmuOFgh|ryPLh?= NWTqWGFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxNFI5QTNpPkKzNVAzQDl|PD;hQi=>
KB NV\kWXVCS3m2b4TvfIlkcXS7IHHzd4F6 MkfoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hQS56MzFOwG0v MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzFyMki5N{c,OjNzMEK4PVM9N2F-
HEK293 M{e2[GlvcGmkaYTpc44hd2ZiaIXtZY4> MYSxMlUhdWmwcx?= NULkZ5lQUW6qaXLpeIlwdiCxZjDoeY1idiCPQWTFNU1u\WSrYYTl[EBcOTSfLX3leIZwem2rbjD1dJRic2ViZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyNjVibXnud{BjgSC|Y3nueIltdGG2aX;uJINwfW62aX7nJIFv[Wy7c3nzMEBKSzVyIE2gNk4yKM7:TT6= Ml[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NEGwNlkoRjJ|MkSxNFI6RC:jPh?=
HEK293 M{TRN2lvcGmkaYTpc44hd2ZiaIXtZY4> M2GwN|MhdWmwcx?= MXPJcohq[mm2aX;uJI9nKGi3bXHuJG9EXDJvbXXkbYF1\WRiQWPQL{B2eHSja3Wg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB|IH3pcpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMj63JO69VS5? NV7ST3g1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOFExOjlpPkKzNlQyODJ7PD;hQi=>
MDCK2 NWTsZpdLUW6qaXLpeIlwdiCxZjDoeY1idg>? M4HlbVUhdWmwcx?= MYTJcohq[mm2aX;uJI9nKGi3bXHuJG1CXEVzLX3l[IlifGWmIGuxOH0udWW2Zn;ycYlvKHWydHHr[UBmgHC{ZYPz[YQhcW5icH;sZZJqgmWmIF3ER2szKGOnbHzzJIFnfGW{IEWgcYlveyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpd{whUUN3MDC9JFQvOyEQvF2u NX[5Upp6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOFExOjlpPkKzNlQyODJ7PD;hQi=>
HEK293 NHjYVXZKdmirYnn0bY9vKG:oIHj1cYFv NIe1ZlZ2eCC2bzC1NFAhfU1? MXyxMlUhdWmwcx?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJG1CXEV{Sz3t[YRq[XSnZDDBV3AsKHWydHHr[UBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJJVxKHSxIEWwNEB2VSCjZoTldkAyNjVibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSB5Lkmg{txONg>? NY\odHdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOFExOjlpPkKzNlQyODJ7PD;hQi=>
HEK293 NFnaR3BKdmirYnn0bY9vKG:oIHj1cYFv NYLX[m1lOS53IH3pcpM> NITxcXZKdmirYnn0bY9vKG:oIHj1cYFvKE2DVFWxMY1m\GmjdHXkJGFUWCtidYD0ZYtmKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNU42KG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hPy57IN88UU4> NFXFSXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K0NVAzQSd-MkOyOFExOjl:L3G+
HEK293 NXnwTIk2UW6qaXLpeIlwdiCxZjDoeY1idg>? M4nINlMhdWmwcx?= M3y1SmlvcGmkaYTpc44hd2ZiaIXtZY4hV0OWMT3t[YRq[XSnZDDBV3AsKHWydHHr[UBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEOgcYlveyCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCyNE45KM7:TT6= NFXXfos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K0NVAzQSd-MkOyOFExOjl:L3G+
H3347 MYnDfZRwfG:6aXPpeJkh[XO|YYm= MkSxO|IhcHK| MlHqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFM{PDdiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBDdHWnIHHzd4F6NCCLQ{WwJF0hPy53MzFOwG0v MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV6M{O1OUc,OjR3OEOzOVU9N2F-
DLD1 NV3WWWRbS3m2b4TvfIlkcXS7IHHzd4F6 NEjsOmE4OiCqcoO= NEL1eWpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCDbHHtZZIhSmy3ZTDhd5NigSxiSVO1NEA:KDJ3LkGg{txONg>? Mlf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEOzOVUoRjJ2NUizN|U2RC:jPh?=
DU145 Moq3R5l1d3SxeHnjbZR6KGG|c3H5 NYfYZVdqPzJiaILz MlrhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSHUyPDViY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFkvOyEQvF2u NIS0b5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwN|k6PSd-MkWwNFM6QTV:L3G+
A549 M1TLVWN6fG:2b4jpZ4l1gSCjc4PhfS=> MlflO|IhcHK| NFnaWG5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUA6NjVizszNMi=> MkfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyMEO5PVUoRjJ3MECzPVk2RC:jPh?=
KB Mmi0R5l1d3SxeHnjbZR6KGG|c3H5 NW\wOpEzPzJiaILz MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iOT64JO69VS5? NHrMfmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwN|k6PSd-MkWwNFM6QTV:L3G+
MDA-MB-435 M1v2[2N6fG:2b4jpZ4l1gSCjc4PhfS=> NYT6NGRlPzJiaILz MojTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iNj6zJO69VS5? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{NkO3PUc,OjZ{Mk[zO|k9N2F-
HCT116 NXTLOlF5S3m2b4TvfIlkcXS7IHHzd4F6 NFGze2s4OiCqcoO= MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFE5NjZizszNMi=> M3PrfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkK2N|c6Lz5{NkKyOlM4QTxxYU6=
A549 M2fCemN6fG:2b4jpZ4l1gSCjc4PhfS=> NGHwWVU4OiCqcoO= MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCyNE4yKM7:TT6= M2D1SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkK2N|c6Lz5{NkKyOlM4QTxxYU6=
HCT116 NE\5W3NCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4qyOVI1KHSxIEeyJIhzew>? NWTRWG9QSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgNlQhfG9iN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IEKg{txONg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUi3OUc,OjZ3OUW4O|U9N2F-
RKO MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M1;v[lI1KHSxIEeyJIhzew>? MW\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKNTzDj[YxteyCjZoTldkAzPCC2bzC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gOk4zKM7:TT6= NIHNcpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVg4PSd-Mk[1PVU5PzV:L3G+
HCT15 MV7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MlXRNlQhfG9iN{KgbJJ{ NIPidohCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFE2KGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB6LkWg{txONg>? NG\1XoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5OVg4PSd-Mk[1PVU5PzV:L3G+
SW480 MojZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NELzdmozPCC2bzC3NkBpenN? NECwcGNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXOFgxKGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSBzMj6zJO69VS5? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7NUi3OUc,OjZ3OUW4O|U9N2F-
PC3 MlPTRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MljKO|IhcHK| NXHBN3Y6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZZN{\XO|ZXSgZZMh[2WubIXsZZIhTE6DIHPvcpRmdnRiYX\0[ZIhPzJiaILzJIJ6KEO7UWXBUnQhVkZiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD64JO69VS5? M1roVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxN|AxLz5{NkezNVMxODxxYU6=
MCF7 Mo\oRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGG2S406PiCqcoO= NGXzNHZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMh[2WubIXsZZIhTE6DIHPvcpRmdnRiYX\0[ZIhQTZiaILzJIJ6KEO7UWXBUnQhVkZiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxKD1iMD65JO69VS5? M2n6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{OxN|AxLz5{NkezNVMxODxxYU6=
T84 NXrybXhtSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUfXeYxsQTZiaILz NF\CWWRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGS4OEBk\WyuczDhd5Nme3OnZDDhd{Bk\WyudXzhdkBFVkFiY3;ueIVvfCCjZoTldkA6PiCqcoOgZpkhS3mTVVHOWEBPTiCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSBzLkKg{txONg>? MnrDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5M{GzNFAoRjJ4N{OxN|AxRC:jPh?=
HT-29 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXPiWYFWPzJiaILz NX;ZcYxGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyClZXzseYxieiCGTlGgZ49vfGWwdDDh[pRmeiB5MjDodpMh[nliQ4nRWWFPXCCQRjDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCxMlkh|ryPLh?= NWjjeYNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3N|E{ODBpPkK2O|MyOzByPD;hQi=>
MCF7 M2CzPGN6fG:2b4jpZ4l1gSCjc4PhfS=> MWKyOEBpenN? MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOC53IN88UU4> NX3ENnFvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4OFEyPjhpPkK2PFQyOTZ6PD;hQi=>
A549 NXrwUW15S3m2b4TvfIlkcXS7IHHzd4F6 NVvJZWd{OjRiaILz NWHhPIc6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiMkSgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IECuPEDPxE1w M{nxXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OESxNVY5Lz5{Nki0NVE3QDxxYU6=
HepG2 NVzVZo5wS3m2b4TvfIlkcXS7IHHzd4F6 M{\COVI1KGi{cx?= MmPYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XluIFnDOVAhRSByLkmg{txONg>? M3L6OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OESxNVY5Lz5{Nki0NVE3QDxxYU6=
SW480 M4XGc2N6fG:2b4jpZ4l1gSCjc4PhfS=> MU[yOEBpenN? Ml\QR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV3c1QDBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XluIFnDOVAhRSBzLkSg{txONg>? NV7tVGFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4OFEyPjhpPkK2PFQyOTZ6PD;hQi=>
A549 M1rLRmN6fG:2b4jpZ4l1gSCjc4PhfS=> MV23NkBpenN? M3XlTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZ49td3KrbXX0dolkKGG|c3H5MEBKSzVyIE2gPU41QCEQvF2u MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7NEi0O{c,OjZ7OUS4OFc9N2F-
KB NYfiT5g{S3m2b4TvfIlkcXS7IHHzd4F6 NX7jOXdpPzJiaILz M4DSZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHPvcI9zcW2ndILpZ{Bie3OjeTygTWM2OCB;IEmuPFI5KM7:TT6= NUD1fWMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVQ5PDdpPkK2PVk1QDR5PD;hQi=>
KB MYjDfZRwfG:6aXPpeJkh[XO|YYm= MUK3NkBpenN? MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhUUN3MDC9JFcvQTlizszNMi=> NU\3cXJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyPFU6OTJpPkK4Nlg2QTF{PD;hQi=>
A549 MVHDfZRwfG:6aXPpeJkh[XO|YYm= M4fsUlczKGi{cx?= MX\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hQC5|IN88UU4> MonZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{OEW5NVIoRjJ6Mki1PVEzRC:jPh?=
MCF7 MVjDfZRwfG:6aXPpeJkh[XO|YYm= MY[3NkBpenN? Mlf3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NEA:KDFzLkOg{txONg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ6NUmxNkc,Ojh{OEW5NVI9N2F-
MDA-MB-231 NYW4eGJwS3m2b4TvfIlkcXS7IHHzd4F6 MonNO|IhcHK| NHvOdJlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwJF0hOTVwNjFOwG0v NFfHbpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK4OVkyOid-MkiyPFU6OTJ:L3G+
HCT116 NUHhSZpHSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVW3NkBpenN? MmGzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IE[uN{DPxE1w NHfDN5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEO1NVU6OCd-MkizOVE2QTB:L3G+
ZR-7530 NXLne5Z5SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NHjhUm04OiCqcoO= MX;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFqULUe1N|Ah[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEG4JO69VS5? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN3MUW5NEc,Ojh|NUG1PVA9N2F-
A549 M1LweWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEmwblU4OiCqcoO= MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDJyIN88UU4> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDN3MUW5NEc,Ojh|NUG1PVA9N2F-
KB NFrUbo9EgXSxdH;4bYNqfHliYYPzZZk> M32x[lczKGi{cx?= NX\MNXNRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFcvQTlizszNMi=> NXTnXXQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3PFk5QTFpPkK4O|g6QDlzPD;hQi=>
A549 NEjpdphEgXSxdH;4bYNqfHliYYPzZZk> MoHIO|IhcHK| NUHUUFNmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hQC5|MTFOwG0v NF7EcIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe4PVg6OSd-Mki3PFk5QTF:L3G+
MCF7 NIrhO2xEgXSxdH;4bYNqfHliYYPzZZk> M2jUSlczKGi{cx?= M4n4NmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFEyNjN{IN88UU4> M{i5WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{i5PFkyLz5{OEe4PVg6OTxxYU6=
MDA-MB-231 NX7PbodHS3m2b4TvfIlkcXS7IHHzd4F6 MXO3NkBpenN? M4jCWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IEG1MlU3KM7:TT6= M2rYblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{i5PFkyLz5{OEe4PVg6OTxxYU6=
KB MkO0R5l1d3SxeHnjbZR6KGG|c3H5 M3;GcFczKGi{cx?= MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iNz65PUDPxE1w M2fkbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUK3O|kxLz5{OEmyO|c6ODxxYU6=
A549 NEfrSINEgXSxdH;4bYNqfHliYYPzZZk> M123W|czKGi{cx?= NV\yZ|U3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hQC5|IN88UU4> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{e5NEc,Ojh7Mke3PVA9N2F-
MCF7 MV3DfZRwfG:6aXPpeJkh[XO|YYm= MYG3NkBpenN? NYLSOoN{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwJF0hOTFwMzFOwG0v MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{e5NEc,Ojh7Mke3PVA9N2F-
MDA-MB-231 NVfFeIpjS3m2b4TvfIlkcXS7IHHzd4F6 NFfuVVk4OiCqcoO= NGPvVmtEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCxOU43KM7:TT6= MofNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mke3PVAoRjJ6OUK3O|kxRC:jPh?=
Maver2 MUDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MkjaO|IhcHK| NU\XblY5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNZZZmejJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPzRizszNMi=> NHzNWGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
NB4 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2XrNlczKGi{cx?= MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6ENDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOSEQvF2u M17jclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUWwN|M2Lz5{OUG1NFM{PTxxYU6=
Capan1 NVfleHBESW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUnCfopMPzJiaILz NGTVNGVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFPhdIFvOSClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNU42KM7:TT6= NFv6WGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
NCI-H460 MljHRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MVq3NkBpenN? MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IOFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTCyMlQh|ryPLh?= MlXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzNUCzN|UoRjJ7MUWwN|M2RC:jPh?=
DU145 NUjkepVTSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1jmWlczKGi{cx?= NUj1Wng{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjC3NkBpenNiYomgV3JDKGG|c3H5MEBKSzVyIE2gNk45KM7:TT6= NF[0bGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
A2780 NFuxTIZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4jCZ|czKGi{cx?= Mlz1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDMke4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMz64JO69VS5? NEXLeYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
HepG2 MnP5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGPzTow4OiCqcoO= NGPIZnpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTC0JO69VS5? NFGzPJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
A431 M4XseWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MYq3NkBpenN? M4m3R2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUSzNUBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iND6xJO69VS5? NYjxO2N[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxOVA{OzVpPkK5NVUxOzN3PD;hQi=>
U2932 MXPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFS1ZVA4OiCqcoO= NYn5cIxXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVNlk{OiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOE41KM7:TT6= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF3MEOzOUc,OjlzNUCzN|U9N2F-
HT-29 MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVW3NkBpenN? MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSB2Lkig{txONg>? NUT6V2k4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxOVA{OzVpPkK5NVUxOzN3PD;hQi=>
HeLa MoDtRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUC3NkBpenN? NWCxRYNySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTC1Mlgh|ryPLh?= NYrCSnE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxOVA{OzVpPkK5NVUxOzN3PD;hQi=>
MM487 MnXNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NX;NNGFWOTBidV2= MoLHO|IhcHK| M3nZNWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV20PFch[2WubIOgZZQhOTBidV2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTDy[YxifGm4ZTD0c{Bkd262cn;sMEBKSzVyIE2gPU41KM7:TT6= NWj1dY9WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxOVA{OzVpPkK5NVUxOzN3PD;hQi=>
MM432 NFzJVJdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MlvINVAhfU1? Mom3O|IhcHK| NWrmS2hXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNUVQ{OiClZXzsd{BifCBzMDD1UUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4wtKEmFNUCgQUAyOCEQvF2u NEPGWZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
A2780/DX NITBNmdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MofnO|IhcHK| M3SwT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUK3PFAwTFhiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFIxNjFizszNMi=> NHvlWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUG1NFM{PSd-MkmxOVA{OzV:L3G+
HCT116 MoS0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1PDblczKGi{cx?= MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB5MjDodpMh[nliU2LCJIF{e2G7LDDJR|UxKD1iMT62N|M1PyEQvF2u MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODFzNUS5Nkc,OzBzMUW0PVI9N2F-
A549 NFzO[ZpCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4GyZ|czKGi{cx?= NFezN5pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IEGuPFgxQTNizszNMi=> Moi2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzMUW0PVIoRjNyMUG1OFkzRC:jPh?=
colon cancer cells MWTDfZRwfG:6aXPpeJkh[XO|YYm= M{LGWmNwdXCxdX7kJJdieyC2ZYP0[YQhcW5idnn0do8h\m:{IHP5eI91d3irY3n0fUBi\2GrboP0JGhEXDFzNjygbJVu[W5iY3;sc44h[2GwY3XyJINmdGy|IDj0ZZhwdC2{ZYPpd5RidnRrIHH0JIEh\HK3ZzDjc45k\W62cnH0bY9vKHC{b3T1Z4lv\yB3MDWgbY5pcWKrdHnvckBw\iClb3zvcpkh\m:{bXH0bY9vNCCLREWwJF0hOC53NDFOwG0v NW\tXHdQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5OlU{PjJpPkGxPVY2OzZ{PD;hQi=>
SW620 NXPY[nhuSW62aYT1cY9zKGG|c3H5 NG\RPWIyOi53IH3nM4to Ml7IRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e2NlAh[2WubIOgfIVvd2e{YX\0[YQhcW5iboXk[UBUS0mGIH3veZNmKGG|c3Xzd4VlKGG|IHTlcIF6KGmwIIT1cY9zKGe{b4f0bEBifCBzMj61JI1oN2upLDDpdC=> NX;uWpNXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVE6OjdpPkKyNVEyQTJ5PD;hQi=>
HT-29 NHzkc3VCdnSrdIXtc5Ih[XO|YYm= MV:2NEBu\y:tZx?= NILzOWozKGSjeYO= NHfzVG5CdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDueYRmKG2xdYPlJIF{e2W|c3XkJIF{KHS3bX;yJIdzd3e2aDDpcohq[mm2aX;uJIF1KDZyIH3nM4toNCCrdjD0bYQh[WSvaX7pd5RmemWmIEKg[IF6eyCvZXHzeZJm\CCxbjDkZZkhOjB? MlP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NkCyPFQoRjJ|M{[wNlg1RC:jPh?=
BT-483 MV7GeY5kfGmxbjDhd5NigQ>? MXGxJJVO NH7RTGszPCCqcoO= MWnJcoR2[3Srb36gc4YhTE6DIHThcYFo\SCrbjDoeY1idiCEVD20PFMh[2WubIOgZZN{\XO|ZXSgZZMh\2GvbXGgTFJCYCCyaH;zdIhwenmuYYTpc44h[XRiU3XyNVM6KGG2IEGgeW0h[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M2DkfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEG5O|M4Lz5{M{SxPVc{PzxxYU6=
HCT116 MWXBcpRqfHWvb4KgZZN{[Xl? NETiblk{OCCvZz;r[y=> MWW2JIRigXN? MVjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgfIVvd2e{YX\0[YQhcW5iboXk[UBud3W|ZTDhd5Nme3OnZDDhd{B{fXK4aY\hcEBifCB|MDDt[{9s\yyrdjDx[EBi\G2rbnnzeIVz\WRiZn;yJFYh\GG7cx?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV6M{O1OUc,OjR3OEOzOVU9N2F-
H3347 Ml\pRY51cXS3bX;yJIF{e2G7 MlzRN|AhdWdxa3e= NWXQfpd[PiCmYYnz MWrBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKM{O0O{Bk\WyuczD4[Y5w\3KjZoTl[EBqdiCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJJN2en[rdnHsJIF1KDNyIH3nM4toNGm4IIHkJIFldWmwaYP0[ZJm\CCob4KgOkBl[Xm| NGrR[HE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW4N|M2PSd-MkS1PFM{PTV:L3G+
HCT116 NF\iPHhCdnSrdIXtc5Ih[XO|YYm= NWiyXHZMOTByIH3nM4to MkTpRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJJhmdm:pcnHmeIVlKGmwIH71M452KG2xdYPlJIF{e2W|c3XkJIF{KHS3bX;yJJJm\3Knc4Ppc44h[XRiMUCwJI1oN2upLDDpekBld3OnZDDvcoNmKGW4ZYL5JJdm\Wt? NGrmUos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCwN|k6PSd-MkWwNFM6QTV:L3G+
NCI-H526 NI\neXpCdnSrdIXtc5Ih[XO|YYm= MYSzNEBu\y:tZx?= MkLXRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJMWg2OjZiY3XscJMhgGWwb3fyZYZ1\WRiaX6gZZRpgW2rYzDueYRmKG2xdYPlJIF1KDNyIH3nM4toNCCrdjDx[EBi\G2rbnnzeIVz\WRiNTD0bY1meyCob4Kg[ZZmenliMjDkZZl{ NXHT[Wc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwOlQxPzhpPkK4NFY1ODd6PD;hQi=>
TC32 NF\zVYdyUFSVIHHzd4F6 NVjNdXd1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGTDN|Ih[2WubIO= NXLn[HJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673 M1jG[JFJXFNiYYPzZZk> NVjzeXVLeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY NWnXO2dteUiWUzDhd5NigQ>? NVu2VI9qeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= NWfzd21uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NUjnOWNMeUiWUzDhd5NigQ>? M{C5NpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= NYXyfGRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 MVHxTHRUKGG|c3H5 MkLZdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy| M2HZNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD MXTxTHRUKGG|c3H5 MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> NFjHOXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 NXT0UHNneUiWUzDhd5NigQ>? MULxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvMUKgZ4VtdHN? NH;oV|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MUTxTHRUKGG|c3H5 NHfST3hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= Ml21QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 Mmf5dWhVWyCjc4PhfS=> MXzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| NETMW|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 NUfoZ5JoeUiWUzDhd5NigQ>? MWPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MlnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 NUDEOI57eUiWUzDhd5NigQ>? NWPs[4dseUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NXjkV2VsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 NIjZXItyUFSVIHHzd4F6 MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORk1GSmNzIHPlcIx{ M3:4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NGT3eIVyUFSVIHHzd4F6 MX\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> M3\HblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 MWTxTHRUKGG|c3H5 MkTndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRnQuOzdiY3XscJM> MkSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) MV7xTHRUKGG|c3H5 M{nkbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ|IDi2MXRIKFJrIHPlcIx{ MmThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 NEHXPVByUFSVIHHzd4F6 NFvVRXRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDNyIHPlcIx{ NF\qNo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 MV7xTHRUKGG|c3H5 MmHudWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgU2hUNTVyIHPlcIx{ NH7lT4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH M3OxZ5FJXFNiYYPzZZk> NUXEeXN2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz Ml7KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 NVvTUplEeUiWUzDhd5NigQ>? MWHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFQyKGOnbHzz NE\UcHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 MW\xTHRUKGG|c3H5 MlzNdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohV3K2aH;nc45idCB|RDD2bYFjcWyrdImgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ M1nVZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) M13UT5FJXFNiYYPzZZk> NI\xTHJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MWXxTHRUKGG|c3H5 MX\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NX\UUFFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MXvxTHRUKGG|c3H5 M{\LSZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz NVm2S3plRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 MWLxTHRUKGG|c3H5 NIm1dY9yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> NVzzXIdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 MoPpdWhVWyCjc4PhfS=> NVzYXZdHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 NX\PSFFueUiWUzDhd5NigQ>? MoXsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? NIq2ZlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 MoK0dWhVWyCjc4PhfS=> NW\SdZM2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| Ml23QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC NEDSd|lyUFSVIHHzd4F6 NGj1bXJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| NUHEbGh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 M4e1NJFJXFNiYYPzZZk> NF7nV3ByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ M3TYXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 NYrCS5d7eUiWUzDhd5NigQ>? NXHvSIlieUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? NGDnSVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 MUXxTHRUKGG|c3H5 MlPjdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV4Fwey1{IHPlcIx{ NX;KWWRERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 MUfxTHRUKGG|c3H5 NGnhcopyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= NVm3cYVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot AMPK / p-AMPK / mTOR / p-mTOR / p70S6K / p-p70S6K ; NFκB p65 / phospho-NFκB p65 / NFκB p50 / IκBα / p27Kip1 ; TopI / pAKT / pMEK / pERK / p-p38 MAPK / pJNK2 25973791 22206574 29237470
Immunofluorescence NFκB 22206574
Growth inhibition assay Cell viability 25973791
In vivo In COLO 320 xenografts, Irinotecan induces a maximum growth inhibition of 92%. [5] A single dose of Irinotecan significantly increases amounts of topoisomerase I covalently bound to DNA in stomach, duodenum, colon and liver. Concomitantly, the Irinotecan-treated group shows significantly higher amounts of DNA strand breaks in colon mucosa cells compared to the control group. [6]

Protocol (from reference)

Cell Research:

[1]
  • Cell lines: LoVo and HT-29 cells
  • Concentrations: 0 μM -100 μM
  • Incubation Time: 48 hours
  • Method:

    Exponentially growing cells (LoVo and HT-29 cells) are seeded in 20 cm2 Petri dishes with an optimal cell number for each cell line (2 × 104 for LoVo cells, 105 for HT-29 cells). They are treated 2 days later with increasing concentrations of Irinotecan or SN-38 for one cell doubling time (24 hours for LoVo cells, 40 hours for HT-29 cells). After washing with 0.15 M NaCl, the cells are further grown for two doubling times in normal medium, detached from the support with trypsin-EDTA and counted in a hemocytometer. The IC50 values are then estimated as the Irinotecan or SN-38 concentrations responsible for 50% growth inhibition as compared with cells incubated without Irinotecan or SN-38.
Animal Research:

[5]
  • Animal Models: Female nude mice with COLO 320 and WiDr xenografts.
  • Dosages: 20 mg/kg
  • Administration: Intraperitoneal injection

Solubility (25°C)

In vitro

 DMSO 100 mg/mL
(147.67 mM)
Water 1 mg/mL
(1.47 mM)
Ethanol '7 mg/mL

Chemical Information

Molecular Weight 677.18
Formula

C33H38N4O6.HCl.3H2O
CAS No. 136572-09-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1=C2CN3C(=CC4=C(C3=O)COC(=O)C4(CC)O)C2=NC5=C1C=C(C=C5)OC(=O)N6CCC(CC6)N7CCCCC7.O.O.O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04158349 Terminated Drug: Oxaliplatin|Drug: mFOLFIRI Colorectal Cancer|Appendiceal Cancer|Peritoneal Carcinoma University of Utah March 22 2021 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Irinotecan (CPT-11) HCl Trihydrate | Irinotecan (CPT-11) HCl Trihydrate supplier | purchase Irinotecan (CPT-11) HCl Trihydrate | Irinotecan (CPT-11) HCl Trihydrate cost | Irinotecan (CPT-11) HCl Trihydrate manufacturer | order Irinotecan (CPT-11) HCl Trihydrate | Irinotecan (CPT-11) HCl Trihydrate distributor