Irinotecan HCl Trihydrate

Catalog No.S2217 Synonyms: CPT-11 HCl Trihydrate

Irinotecan HCl Trihydrate Chemical Structure

Molecular Weight(MW): 677.18

Irinotecan prevents DNA from unwinding by inhibition of topoisomerase 1.

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In DMSO USD 190 In stock
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Cited by 14 Publications

4 Customer Reviews

  • Growth inhibitory effects of Irinotecan in human pancreatic cancer cells. Panc1 cells were plated in triplicates into 48-well plates at a density of 10,000 cells/ml. After 24 hours, complete culture medium was changed into fresh low-serum-containing medium (1% FBS) containing DMSO (control) or indicated doses of Irinotecan (Selleckchem). Cell viability 72 hours after treatment was determined by AlamarBlue assay (Invitrogen) according to manufacturer's instructions. Results are expressed as percentages of control, which was arbitrarily assigned 100% viability, and represented as the mean ± standard deviation (SD) of the tripicate wells. 

    Dr. Mikhail Menshikov of Cardiology Research Center. Irinotecan HCl Trihydrate purchased from Selleck.

  • In vitro cell uptake data showing the HA coating on HAC-PFP-DC nanoparticles significantly improves drug delivery into prostasphere and mammosphere cells enriched with CSCs. Fluorescence micrographs of (A) prostasphere and (B) mammosphere cells after incubated with the simple mixture of free DOX&CPT, PFP-DC nanoparticles, and HAC-PFP-DC nanoparticles for 3 h at 37 °C. CPT:irinotecan

    Biomaterials, 2015, 72:74-89. Irinotecan HCl Trihydrate purchased from Selleck.

  • Monolayer cultures of human OVCAR-5 cells were incubated with Mn, irinotecan or their combination for 6, 24, and 48 hours. Representative immunofluorescence imaging of Tdp1 (red fluorescence) and γH2AX (green fluorescence) in OVCAR-5 cells subjected to (i) No-treatment (NT); (ii) Mn ; (iii) Irinotecan; and (iv) Combination of Mn and irinotecan for 24 hours.

    Mol Cancer Ther, 2018, 17(2):508-520. Irinotecan HCl Trihydrate purchased from Selleck.

  • (A) HCT116 and (B) HT29 cells were cultured with 0.1% DMSO (control) or A452 (0.5, 1, 2 uM), SAHA (5 uM), cisplatin (10 uM), irinotecan (5 uM), or capecitabine (10 uM) at the indicated concentrations for 24 h. The Western blot analysis shows PARP degradation, proapoptotic and antiapoptotic markers. α-Tubulin is shown as a loading control.

    Carcinogenesis, 2018, 39(1):72-83. Irinotecan HCl Trihydrate purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Irinotecan prevents DNA from unwinding by inhibition of topoisomerase 1.
Features Irinotecan is a prodrug that is used to treat metastatic colorectal cancer.
Targets
Topo I [1]
In vitro

Irinotecan is activated to SN-38 by carboxylesterases to become able to interact with its target, topoisomerase I. Irinotecan induces similar amounts of cleavable complexes at its IC50 in LoVo cells and HT-29 cell lines. SN-38 induces a concentration-dependent formation of cleavable complexes, which is not significantly different in LoVo cells and HT-29 cell lines. Cell accumulation of Irinotecan is markedly different, reaching consistently higher levels in HT-29 cells than in LoVo cells. [1] The lactone E-ring of Irinotecan and SN-38 hydrolyses reversibly in aqueous solutions, and the interconversion between the lactone and carboxylate forms is dependent on pH and temperature. Liver is primarily responsible for the activation of Irinotecan to SN-38. At equal concentrations of Irinotecan and SN-38 glucuronide, the rate of beta-glucuronidase-mediated SN-38 production is higher than that formed from Irinotecan in both tumour and normal tissue. [2] Irinotecan is also converted to SN-38 in intestines, plasma and tumor tissues. [3] Irinotecan is significantly more active in SCLC than in NSCLC cell lines, whereas no significant difference between histological types is observed with SN-38. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse bone marrow cell MmHKR5l1d3SxeHnjbZR6KGG|c3H5 Mo\XR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgZo9v\SCvYYLyc5ch[2WubDDifUBETlVvR12gZZN{[XlwIFnDOVA:OC5yMEGg{txO NFLGbZgzOTN2MU[3OC=>
PC3 cells M{HCOWN6fG:2b4jpZ4l1gSCjc4PhfS=> NY\vSXluPzJiaB?= NHnxXZNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNFQh|ryP MYSyNVM1OTZ5NB?=
A375 cells MVrDfZRwfG:6aXPpeJkh[XO|YYm= NFjXRos4OiCq MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeT6gTWM2OD1yLkC0JO69VQ>? MWSyNVM1OTZ5NB?=
LNCAP cells M4i1WGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVraXm9nPzJiaB?= Mn7oR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUG5ESVBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNFkh|ryP M4jkPVIyOzRzNke0
MESSA cells M3XKR2N6fG:2b4jpZ4l1gSCjc4PhfS=> MXm3NkBp NYLjRZdMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUWVU1GgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NiCLQ{WwQVAvODFizszN M3zMclIyOzRzNke0
H460 cells M1PWTGN6fG:2b4jpZ4l1gSCjc4PhfS=> MnnkO|IhcA>? MnTDR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFQ3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmuJGlEPTB;MD6wNVUh|ryP NIXhSYgzOTN2MU[3OC=>
MES-SA/Dx5 cells NIn4U|lEgXSxdH;4bYNqfHliYYPzZZk> MnPuO|IhcA>? NF3iUGFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTVNvU1GvSJg2KGOnbHzzJI93\XKneIDy[ZN{cW6pIF3EVlEh[W[2ZYKgO|IhcHK|IHL5JIFt[W2jcjDicJVmKGG|c3H5MkBKSzVyPUCuNFIzKM7:TR?= M4[4cVIyOzRzNke0
H69 cells NWrZOZJmS3m2b4TvfIlkcXS7IHHzd4F6 M1;teFczKGh? NVTVSVBmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDZ7IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlAzOiEQvF2= MXmyNVM1OTZ5NB?=
IGROV1 cells NHvifWtEgXSxdH;4bYNqfHliYYPzZZk> NVjpfW5wPzJiaB?= MmrmR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTWdTV1ZzIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlA{KM7:TR?= NH22W40zOTN2MU[3OC=>
SK-MEL-2 cells NH7kTpdEgXSxdH;4bYNqfHliYYPzZZk> NGjuSGk4OiCq MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMjDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZkvKEmFNUC9NE4yKM7:TR?= MX6yNVM1OTZ5NB?=
MALME-3M cells NELJfXJEgXSxdH;4bYNqfHliYYPzZZk> NEnDdZE4OiCq NHjwZ2xEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOSUyPRT2zUUBk\WyuczDh[pRmeiB5MjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XlwIFnDOVA:OC5{IN88US=> MXOyNVM1OTZ5NB?=
DU145 cells NGrFSXZEgXSxdH;4bYNqfHliYYPzZZk> MXq3NkBp NELCRpFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZkvKEmFNUC9NE4zKM7:TR?= NXnv[JE{OjF|NEG2O|Q>
HT-29 cells M1TB[WN6fG:2b4jpZ4l1gSCjc4PhfS=> NWjsbmNSPzJiaB?= M3zhU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfU4hUUN3ME2wMlIzKM7:TR?= NH\JWW4zOTN2MU[3OC=>
H69AR cells NFX6OJJEgXSxdH;4bYNqfHliYYPzZZk> NFXiSI44OiCq NF\ZXWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJPjmDUjDj[YxteyCxdnXy[ZhxemW|c3nu[{BOTFJzIHHmeIVzKDd{IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigS5iSVO1NF0xNjJ3IN88US=> M4Ti[VIyOzRzNke0
MCF7 cells NYWybml6S3m2b4TvfIlkcXS7IHHzd4F6 NWrUenAxOjRiaB?= M4S3XWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XlwIFnDOVA:OC53IN88US=> NUTsU3p6OjZ6NEGxOlg>
HCT116 MW\GeY5kfGmxbjDhd5NigQ>? M4jqW2NwdXCxdX7kJJdieyC2ZYP0[YQhcW5idnn0do8h\m:{IHP5eI91d3irY3n0fUBi\2GrboP0JGhEXDFzNjygbJVu[W5iY3;sc44h[2GwY3XyJINmdGy|IDj0ZZhwdC2{ZYPpd5RidnRrIHH0JIEh\HK3ZzDjc45k\W62cnH0bY9vKHC{b3T1Z4lv\yB3MDWgbY5pcWKrdHnvckBw\iClb3zvcpkh\m:{bXH0bY9vNiCLQ{WwQVAvPTRizszN MWKxNVk3PTN4Mh?=
RPMI8402 NUnMNmlXS3m2b4TvfIlkcXS7IHHzd4F6 M{HCOVQh\GG7cx?= Mn7qR5l1d3SxeHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5ibIntdIhw[myjc4SgeJVud3JiY3XscEBtcW6nIGLQUWk5PDB{IHHmeIVzKDRiZHH5d{Bw\iC2cnXheI1mdnRwIFnDOVA:OC53NzFOwG0> NGT3TYEyOjd2N{e5PC=>
KB3-1 cells MnzMR5l1d3SxeHnjbZR6KGG|c3H5 M1fWdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDOy1zIHPlcIx{KGK7IF3UWEBu\XSqb3SuJGlEPTB;MD62PEDPxE1? M3OwOlE5PzdzOUOw
PC3 cells MUnQdo9tcW[ncnH0bY9vKGG|c3H5 MnLxO|IhcA>? NV7D[XhLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZZN{\XO|ZXSgZZMh[2WubIXsZZIhTE6DIHPvcpRmdnRiYX\0[ZIhPzJiaILzJIJ6KEO7UWXBUnQhVkZiZnz1c5Jme2OnbnPlJIF{e2G7LjDJR|UxRTBwODFOwG0> MWqyOlc{OTNyMB?=
A549 cells Ml\jR5l1d3SxeHnjbZR6KGG|c3H5 MVSyOEBp M2S4TWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XlwIFnDOVA:OC56IN88US=> MVGyOlg1OTF4OB?=
MCF7 cells NGjJcYZRem:uaX\ldoF1cW:wIHHzd4F6 MkPtPVYhcA>? MnrDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJINmdGy3bHHyJGRPSSClb370[Y51KGGodHXyJFk3KGi{czDifUBEgVGXQV7UJG5HKG[udX;y[ZNk\W6lZTDhd5NigS5iSVO1NF0xNjlizszN MUWyOlc{OTNyMB?=
HepG2 cells NWLrcXl7S3m2b4TvfIlkcXS7IHHzd4F6 MX6yOEBp MkDnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCPVGOgZZN{[XlwIFnDOVA:OC57IN88US=> MYKyOlg1OTF4OB?=
MDA-MB-435 cells MWTDfZRwfG:6aXPpeJkh[XO|YYm= MXO3NkBp Ml3RR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LjDJR|UxRTFwMUSg{txO M{PGO|IxOzdzMUiz
LS174T cells NVKwSmx5S3m2b4TvfIlkcXS7IHHzd4F6 MnvZPVYhcA>? MljpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMyPzSWIHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZkvKEmFNUC9NU4yPiEQvF2= NUn6TVNwOTh3MUO5O|Y>
T84 cells M2rnNXBzd2yrZnXyZZRqd25iYYPzZZk> M{n0RVk3KGh? MkLCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCWOESgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdHWuYYKgSG5CKGOxboTlcpQh[W[2ZYKgPVYhcHK|IHL5JGN6WVWDTmSgUmYh\my3b4Lld4NmdmOnIHHzd4F6NiCLQ{WwQVEvOiEQvF2= NFn1NXkzPjd|MUOwNC=>
SW480 cells M3zRdmN6fG:2b4jpZ4l1gSCjc4PhfS=> M1S3VFI1KGh? NEDy[HhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUXzR6MDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiMkSgbJJ{KGK7IF3UV{Bie3OjeT6gTWM2OD1zLkSg{txO NVTwUYR3OjZ6NEGxOlg>
K562 cells Ml3kVJJwdGmoZYLheIlwdiCjc4PhfS=> MofhO|IhcA>? MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhPzJiaILzMkBKSzVyPUGuPUDPxE1? MWOyOVQzODF5NR?=
HT-29 cells Moj2VJJwdGmoZYLheIlwdiCjc4PhfS=> M{f2NlczKGh? MorQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyudXzhdkBFVkFiY3;ueIVvfCCjZoTldkA4OiCqcoOgZpkhS3mTVVHOWEBPTiCobIXvdoV{[2WwY3WgZZN{[XlwIFnDOVA:OS57IN88US=> M4HyO|I3PzNzM{Cw
HCT116 cells NIiy[HlRem:uaX\ldoF1cW:wIHHzd4F6 NWLTUmJsOjRvN{KgbC=> NX:wXVR4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgNlQhfG9iN{KgbJJ{KGK7IGPSRkBie3OjeT6gTWM2OD1{IN88US=> NEjPNFEzPjV7NUi3OS=>
Hep3B cells NIrFNGhRem:uaX\ldoF1cW:wIHHzd4F6 M3rl[lczKGh? M{HF[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwN2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGjUWEBie3OjeT6gTWM2OD12LkezJO69VQ>? NFrwSIwyQTd7Nkm1Oi=>
Hep3B cells M3jF[mdzd3e2aDDpcohq[mm2b36gZZN{[Xl? NWX4[Fl7PzJiaB?= M1\ZT2dzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhmeDOEIHPlcIx{KGGodHXyJFczKGi{czDifUBZXFRiYYPzZZkvKEmFNUC9OE44OyEQvF2= NVLZSoVzOjJyN{myOVQ>
LoVo cells M1fSSWN6fG:2b4jpZ4l1gSCjc4PhfS=> MY[3NkBp MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmuJGlEPTB;ND65PUDPxE1? M4K5R|IxOzdzMUiz
KBH5.0 cells NETse2pEgXSxdH;4bYNqfHliYYPzZZk> MoKxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2JJPS5yIHPlcIx{KGK7IF3UWEBu\XSqb3SuJGlEPTB;Nz60JO69VQ>? Mn\DNVg4PzF7M{C=
H3347 cells NILMR2xEgXSxdH;4bYNqfHliYYPzZZk> MX:3NkBp Ml;GR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFM{PDdiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBDdHWnIHHzd4F6NiCLQ{WwQVcvPTNizszN MofNNlQ2QDN|NUW=
HCT15 cells M4no[HBzd2yrZnXyZZRqd25iYYPzZZk> NFPpOmkzPC15MjDo NGf1cHlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFE2KGOnbHzzJIFnfGW{IEK0JJRwKDd{IHjyd{BjgSCVUlKgZZN{[XlwIFnDOVA:QC53IN88US=> NWLjUXBLOjZ3OUW4O|U>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
AMPK / p-AMPK / mTOR / p-mTOR / p70S6K / p-p70S6K; 

PubMed: 25973791     


Western blot showing the protein levels of AMPK, p-AMPK, mTOR, p-mTOR, p70S6K, and p-p70S6K in LoVo and LoVo-R8 with or without irinotecan. 

NFκB p65 / phospho-NFκB p65 / NFκB p50 / IκBα / p27Kip1; 

PubMed: 22206574     


For Western blot analysis (B), cytoplasmic proteins were analyzed using antibodies against NFκB p65 and p50, phospho-NFκB p65, IκBα and p27Kip1. In the presence of irinotecan, there was a loss of cytoplasmic NF-κBp65, but in the presence of sorafenib, this loss was greatly reduced, corresponding to a decrease in phosphorylation of NF-κBp65. In addition, compared to treatment with sorafenib or irinotecan alone, there was increased expression of IκBα following treatment with sorafenib and irinotecan. Lastly, following treatment with irinotecan and sorafenib irinotecan combination, there was decreased expression of p27Kip1 compared to sorafenib treatment alone.

TopI / pAKT / pMEK / pERK / p-p38 MAPK / pJNK2; 

PubMed: 29237470     


a Gimatecan significantly inhibited the expression of TopI, pAKT, pMEK, and pERK, and activated the expression of p-p38 MAPK and pJNK2 in SNU-1 cells. b Gimatecan significantly inhibited the expression of pAKT and pERK in NCI-N87 cells. Cells were starved in serum-free medium overnight, exposed to gimatecan or irinotecan for 48 h and harvested at 70–80% confluence. Total protein of SNU-1 and NCI-N87 was extracted and the expression of TopI, pAKT, pMEK, pERK, p-p38 MAPK and pJNK2 were assessed by western-blotting 

25973791 22206574 29237470
Immunofluorescence
NFκB; 

PubMed: 22206574     


BT12 cells were incubated with sorafenib or vehicle for 30 minutes followed by treatment with irinotecan for an additional 2 hours. For indirect immunofluorescence (A), cells were fixed and stained with antibodies to NF-κB followed by fluorescent labeled secondary antibodies. Concurrent DAPI stain was used to localize the nuclei (lower panel). Slides were visualized using a fluorescent microscope and random fields were photographed. The cytoplasmic staining seen in untreated and sorafenib treated cells was significantly reduced following treatment with irinotecan. However, the addition of sorafenib enabled the cells to maintain cytoplasmic staining in the presence of irinotecan. 

22206574
Growth inhibition assay
Cell viability; 

PubMed: 25973791     


The sensitivity of eight colon cancer cell lines to irinotecan was measured using the CCK-8 assay. For the CCK-8 assay, cells were exposed to irinotecan at given concentrations for 72 h before measurement. The cell viability was presented as the percentage relative to untreated cells.

25973791
In vivo In COLO 320 xenografts, Irinotecan induces a maximum growth inhibition of 92%. [5] A single dose of Irinotecan significantly increases amounts of topoisomerase I covalently bound to DNA in stomach, duodenum, colon and liver. Concomitantly, the Irinotecan-treated group shows significantly higher amounts of DNA strand breaks in colon mucosa cells compared to the control group. [6]

Protocol

Cell Research:

[1]
+ Expand
  • Cell lines: LoVo and HT-29 cells
  • Concentrations: 0 μM -100 μM
  • Incubation Time: 48 hours
  • Method:

    Exponentially growing cells (LoVo and HT-29 cells) are seeded in 20 cm2 Petri dishes with an optimal cell number for each cell line (2 × 104 for LoVo cells, 105 for HT-29 cells). They are treated 2 days later with increasing concentrations of Irinotecan or SN-38 for one cell doubling time (24 hours for LoVo cells, 40 hours for HT-29 cells). After washing with 0.15 M NaCl, the cells are further grown for two doubling times in normal medium, detached from the support with trypsin-EDTA and counted in a hemocytometer. The IC50 values are then estimated as the Irinotecan or SN-38 concentrations responsible for 50% growth inhibition as compared with cells incubated without Irinotecan or SN-38.


    (Only for Reference)
Animal Research:

[5]
+ Expand
  • Animal Models: Female nude mice with COLO 320 and WiDr xenografts.
  • Formulation: 0.9% NaCl
  • Dosages: 20 mg/kg
  • Administration: Intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (147.67 mM)
Ethanol 7 mg/mL (10.33 mM)
Water 1 mg/mL (1.47 mM)
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+saline
For best results, use promptly after mixing. 		20mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 677.18
Formula

C33H38N4O6.HCl.3H2O
CAS No. 136572-09-3
Storage powder
in solvent
Synonyms CPT-11 HCl Trihydrate

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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04074343 Recruiting Drug: TAS-102|Drug: Irinotecan Gastric Adenocarcinoma|GastroEsophageal Cancer University of California Irvine|Taiho Pharmaceutical Co. Ltd. August 28 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID