Estradiol

Catalog No.S1709 Synonyms: 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol

For research use only.

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

Estradiol Chemical Structure

CAS No. 50-28-2

Selleck's Estradiol has been cited by 11 Publications

Purity & Quality Control

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Biological Activity

Description Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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UAS NHP0eWNHfW6ldHnvckBie3OjeR?= MV:xPEBpenN? NUe1enB[SWexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEh\XiycnXzd4VlKGmwIGXBV{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGKndHGtcIFkfGGvYYPlJIFnfGW{IEG4JIhzeyCkeTDi[ZRiNWyjY4ThcYF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNlXPxE1? NI\OfpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi4OVI4QSd-MkG4PFUzPzl:L3G+
HEC-1 MVPGeY5kfGmxbjDhd5NigQ>? M3X4WFI1KGi{cx?= NX;GOWY5SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCKRVOtNUBk\WyuczDjc4V5eHKnc4Ppcoch[mW2YT3nZYwh[XO|ZYPz[YQh[XNibIXjbYZmemG|ZTDhZ5Rqfmm2eTDh[pRmeiB{NDDodpMh[nlicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwNFEzO87:TR?= NHjDc|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGyNlU3Oyd-MkKxNlI2PjN:L3G+
U2OS NYD2ellJTnWwY4Tpc44h[XO|YYm= NGPqelkzPCCqcoO= MWfB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJHUzV1NiY3XscJMh[2:neIDy[ZN{cW6pIIDDUXgucFOUQ{OgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMEG1{txO MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
HEC-1 MkDySpVv[3Srb36gZZN{[Xl? MYiyOEBpenN? NYjRRVNZSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGg[ZhxemW|c3XkJIlvKEiHQz2xJINmdGy|IHPv[ZhxemW|c3nu[{Bj\XSjLXfhcEBie3Onc4Pl[EBieyCudXPp[oVz[XOnIHHjeIl3cXS7IHHmeIVzKDJ2IHjyd{BjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNu69VQ>? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
U2OS NFTlOG5HfW6ldHnvckBie3OjeR?= M1rsU|I1KGi{cx?= MVnB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gWVJQWyClZXzsd{Bkd2W6cILld5NqdmdicFPNXE1pW1KFMzDhd5Nme3OnZDDhd{BtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByNN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
BL21 M2nubmZ2dmO2aX;uJIF{e2G7 M375[lEhcHJ? NVPPVFlxSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBPNUirc{[teIFo\2WmIITldoJqfW1vbHHi[Yxt\WRiTmLJSE1UWkN|IH;mJGVT[WyyaHGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZYZ1\XJiMTDodkBjgSCWUj3GVmVVKGG|c3H5MEBGSzVyPUCuNFAxPTcQvF2= MnTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
BL21 MmKxSpVv[3Srb36gZZN{[Xl? NFvqco8yKGi{ M2L6cWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKE6USVStV3JEOyCxZjDFVoJmfGFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCHc3Po[ZJq[2irYTDjc4xqKEKOMkGgZ4VtdHNiYX\0[ZIhOSCqcjDifUBVWi2IUlXUJIF{e2G7LDDFR|UxRTBwMECwPe69VQ>? NID4Xok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGyNlU3Oyd-MkKxNlI2PjN:L3G+
BL21 NWXs[VVJTnWwY4Tpc44h[XO|YYm= M4GxW|EhcHJ? M1jYbmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKEWUYnX0ZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{Bie3Onc4Pl[EBieyC{ZXPyeYl1dWWwdDDv[kBndHWxcnXzZ4Vqdi2uYXLlcIxm\CCVUlOzJIFnfGW{IEGgbJIh[nliVGKtSnJGXCCjc4PhfUwhTUN3ME2wMlAxOTYQvF2= MnHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
BL21 M{HidmZ2dmO2aX;uJIF{e2G7 NEjnR3cyKGi{ MnPpRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDOMWhqezZvdHHn[4VlKHSncnLpeY0udGGkZXzs[YQhTVKjbIDoZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSClZXzsd{Bie3Onc4Pl[EBieyC{ZXPyeYl1dWWwdDDv[kBndHWxcnXzZ4Vqdi2uYXLlcIxm\CCVUlOzJIFnfGW{IEGgbJIh[nliVGKtSnJGXCCjc4PhfUwhTUN3ME2wMlAxO87:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
Ishikawa cells MoKySpVv[3Srb36gZZN{[Xl? NHf6[|U{KGSjeYO= NXPvPIlvSWexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBt\X[nbDDh[pRmeiB|IHThfZMhfXOrbnegVE1vcXS{b4Do[Y56dCCyaH;zdIhifGViYYOgd5Vje3S{YYTlMEBGSzVyPUCuNFAxQc7:TR?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF5OEW1Nkc,OjJzN{i1OVI9N2F-
HepG2 NHXLUVdHfW6ldHnvckBie3OjeR?= MXyyOEBpenN? MWXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEKy{txO M3T5NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN|I5Lz5{MkK4N|MzQDxxYU6=
HepG2 MlLySpVv[3Srb36gZZN{[Xl? MYWyOEBpenN? MnTsRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUJOwG0> MoXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OEOzNlgoRjJ{MkizN|I5RC:jPh?=
MCF7 M2fEcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXW3NkBpenN? MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwLXTl[olkcWWwdDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:OC5yMECwNFY1|ryP Mn;nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkS2PFEoRjJ{NE[0OlgyRC:jPh?=
MCF7:D5L M4nKbWZ2dmO2aX;uJIF{e2G7 NI\keY0yPiCqcoO= NFPiVFdGe3S{b3flcolkKGGldHn2bZR6KGG2IHXzeJJw\2WwIILlZ4VxfG:{IHnuJIh2dWGwIF3DSlc7TDWOIHPlcIx{KGOxLXX4dJJme3OrbnegSXJGKGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBtfWOrZnXyZZNmKGenbnWg[ZhxemW|c3nvckBi\nSncjCxOkBpenNiYomgcYlkem:ybHH0[UBz\WGmZYKsJGVEPTB;MD6wNFAxOTl4zszN M1r4cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[0OlgyLz5{MkS2OFY5OTxxYU6=
Ishikawa cells M4i1S2Z2dmO2aX;uJIF{e2G7 NFPYR244OiCqcoO= NFm4TpNGe3S{b3flcolkKGGldHn2bZR6KGG2IHXzeJJw\2WwIILlZ4VxfG:{IHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBidGujbHnu[UBxcG:|cHjheIF{\SCneIDy[ZN{cW:wIHHmeIVzKDd{IHjyd{BjgSCvaXPyc5Bt[XSnIILlZYRmeixiRVO1NF0xNjByMECyOFbPxE1? M2rPbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[0OlgyLz5{MkS2OFY5OTxxYU6=
Ishikawa cells M1H6[WZ2dmO2aX;uJIF{e2G7 NXHjSHFTOC5zIH7N M3HMSlczKGi{cx?= MkfuSYZn\WO2IH;uJINmdGxicILvcIln\XKjdHnvckBw\iCqdX3hckBKe2ira3H3ZUBk\WyuczDheEAxNjFibl2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?= NVrmcHF1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
CHO-K1 Mk\CSpVv[3Srb36gZZN{[Xl? NHjqZ3IzPCCqcoO= MmXzWJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gc4YhTVKkZYThJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tT|Eh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxODB|Nt88US=> M33LT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O|IyPzxxYU6=
CHO-K1 NHPvdIFHfW6ldHnvckBie3OjeR?= MkPxNlQhcHK| MnHTWJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gc4YhTVKjbIDoZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPMWsyKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODByNd88US=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109 MWPGeY5kfGmxbjDhd5NigQ>? MWCyOEBpenN? NGq3PYhC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhVEKGIHX4dJJme3OnZDDpckB6\WG|dDDBTFExQSClZXzsd{Bie3Onc4Pl[EBieyClb33wc5Vv\CClb37j[Y51emG2aX;uJIF1KHeqaXPoJFExLSCjZ3;ubZN1KGGldHn2bZR6KG:oIITo[UBu[XirbYXtJIFkfGm4aYT5JI9nKEV{IHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCDY4Tpeol1gT1yLkCwNFHPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109 NVi0dohkTnWwY4Tpc44h[XO|YYm= NX;nTVJQOjRiaILz M1ix[mFod26rc4SgZYN1cX[rdImgZZQhTVKkZYThJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMh[2:vcH;1coQh[2:wY3XueJJifGmxbjDheEB4cGmlaDCxNEUh[WexbnnzeEBi[3Srdnn0fUBw\iC2aHWgcYF5cW23bTDhZ5Rqfmm2eTDv[kBGOiCjZoTldkAzPCCqcoOgZpkh[WyyaHGt[4Ft[WO2b4Pp[IF{\SCjc4PhfUwhSWO2aY\peJk:OC5yMECxPO69VQ>? MmrkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109 NXXl[2o1TnWwY4Tpc44h[XO|YYm= NYjmXHdWOjRiaILz MnOxRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCHQ{WwQVAvODBzMEZOwG0> M{HLU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O|IyPzxxYU6=
yeast AH109 NIP1XGJHfW6ldHnvckBie3OjeR?= M2TudFI1KGi{cx?= MUXB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDy[YNmeHSxcjDMRmQh\XiycnXzd4VlKGmwIInlZZN1KEGKMUC5JINmdGy|IHHzd4V{e2WmIHHzJIlvfGW{YXP0bY9vKHerdHigV3JEOSCjZoTldkAzPCCqcoOgZpkh[WyyaHGt[4Ft[WO2b4Pp[IF{\SCjc4PhfUwhTUN3ME2wMlAxOTB6zszN NGLmSWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O|IyPyd-MkK2OFczOTd:L3G+
U2-OS NFzBZolHfW6ldHnvckBie3OjeR?= NXLOd3VjSWO2aY\heIlwdiCxZjDoeY1idiCHUnHsdIhiKGW6cILld5Nm\CCrbjDoeY1idiCXMj3PV{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGVEPTB;MD6wNFAxODUQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
U2-OS NIDhcGdHfW6ldHnvckBie3OjeR?= MUPBZ5RqfmG2aX;uJI9nKGi3bXHuJGVT[mW2YTDlfJBz\XO|ZXSgbY4hcHWvYX6gWVIuV1NiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5JJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9tNCCHQ{WwQVAvODByMEJOwG0> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
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Assay
Methods Test Index PMID
Western blot p-GSK3β / GSK3 ; β-catenin ; Survivin / ER-alpha / NgBR ; ERα / FOXP1 / KRAS / PTEN 19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 54 mg/mL
(198.25 mM)
Water Insoluble
Ethanol '5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05175105 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05144256 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05096169 Not yet recruiting Drug: Clomiphene Citrate 25mg|Drug: Clomiphene Citrate 50mg Testosterone Deficiency Weill Medical College of Cornell University December 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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