Estradiol

Catalog No.S1709 Synonyms: 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol

For research use only.

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

Estradiol Chemical Structure

CAS No. 50-28-2

Selleck's Estradiol has been cited by 12 Publications

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Other Estrogen/progestogen Receptor Products

Biological Activity

Description Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 NWfmW4xmTnWwY4Tpc44h[XO|YYm= NF3zelNFcXOybHHj[Y1mdnRib3[gX|NJZWW|dILh[IlwdCCocn;tJGV{fHKxZ3XuJJJm[2WydH;yJIlvKE2FRj23JINmdGy|LDDJR|UxRTBwMECz{txO MkjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2OE[4N{c,OTV2OE[4N|ww[T5?
MCF-7 NXfESoFDTnWwY4Tpc44h[XO|YYm= NEX4OopG\m[nY4TpeoUh\G:|ZTDmc5IhYzOKXT2g[ZN1emGmaX;sJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDNR2YuPyClZXzsd{whTUN3ME2wMlAxODB{zszN NWW4dZVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PVY5OSd-Mke2PVY5OTxxYU6=
Ishikawa cells MkDFSpVv[3Srb36gZZN{[Xl? NFnLOIVEd26lZX70doF1cW:wIH;mJINwdXCxdX7kJJJmeXWrcnXkJJRwKGmwZIXj[UA2OCBnIH;mJJRp\SCvYYjpcZVuKHO2aX31cIF1cW:wIH;mJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGGldHn2bZR6KGmwIFnzbIls[XejIHPlcIx{NCCHQ{WwQVAvODByMEJOwG0> NVH0TG9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEKwNVU5Pyd-OEKwNVU5PzxxYU6=
MCF-7 NFXNe4dHfW6ldHnvckBie3OjeR?= M3nUS2lv\HWldHnvckBw\iCyU{KgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIF3DSk04KG2jbX3hdpkh[2G{Y3nuc41iKGOnbHzzMEBGSzVyPUCuNFAxODl7Nt88US=> NUPKUJdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOE[yO|YyPSd-OE[yO|YyPTxxYU6=
MCF-7 NFzDWXFHfW6ldHnvckBie3OjeR?= MnzzTY5lfWO2aX;uJI9nKHCVMjDtVm5CKGW6cILld5Nqd25iaX6gbJVu[W5iTVPGMVchdWGvbXHyfUBk[XKlaX7vcYEh[2WubIOsJGVEPTB;MD6wNFAzQTMQvF2= MmO0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDZ{N{[xOUc,QDZ{N{[xOVww[T5?
mammary cancer cells MnTDSpVv[3Srb36gZZN{[Xl? MWDB[oZqdmm2eTDld5Rzd2enbjDy[YNmeHSxcjDv[kBOS0ZvNzDoeY1idiCvYX3tZZJ6KGOjbnPldkBk\WyuczygSWM2OD1yLkCwN|jPxE1? NWfPWYF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOE[yO|YyPSd-OE[yO|YyPTxxYU6=
HeLa M1r0T2Z2dmO2aX;uJIF{e2G7 MX;UVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBGOjF5YnX0ZWchfXC2YXvlJIlvKE:jdICxMYV5eHKnc4PpcochUGWOYTDj[YxteyxiS3m9OE44|ryP NGXPO489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96N{e5PFk1Lz56N{e5PFk1RC:jPh?=
MCF-7 MYjGeY5kfGmxbjDhd5NigQ>? M4XNTmdzd3e2aDDy[ZNxd26|ZTDpckBkfWy2dYLld{Bw\iCPQ1[tO{ApcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmVrIHPlcIx{NCCHQ{WwQVAvODByMECxOe69VQ>? NX:4NGRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUO1O|U{Oyd-OUO1O|U{OzxxYU6=
MCF-7 M{Dub2Z2dmO2aX;uJIF{e2G7 MXLJcoR2[3Srb36gc4YheFN{IFflcoUh\XiycnXzd4lwdiCrbjDoeY1idiCPQ1[tO{Bk\WyuczygSWM2OD1yLkCwNFA{ODIQvF2= NGHJb2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97M{exNlQyLz57M{exNlQyRC:jPh?=
MCF-7 NYC1WI5qTnWwY4Tpc44h[XO|YYm= M3HNXGFn\mmwaYT5JIZweiCnc4Tyc4dmdiC{ZXPldJRweiCxZjDoeY1idiCPQ1[tO{Bk\WyuczygSWM2OD1yLkCwNFE5|ryP NUT3RoNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUO3NVI1OSd-OUO3NVI1OTxxYU6=
HRPE NXLoO5RjTnWwY4Tpc44h[XO|YYm= MYrUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBVTUFidYD0ZYtmKCiWRVG6JFIxKHWPKTDpckBQS1R|LXX4dJJme3OrbnegTHJRTSClZXzsd{whUUN3ME2xMlHPxE1? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDNyMEKyK|46QDNyMEKyQE9iRg>?
MCF-7 NVnCS|BQTnWwY4Tpc44h[XO|YYm= MkTzRYdwdmm|dDDl[oZm[3Rib36geJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gbY4hVUOILUegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[SxiRVO1NF0xNjByMECwO:69VQ>? MkXWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB4N{OwPVkoRjFyNkezNFk6RC:jPh?=
HeLa NVnKO5R1TnWwY4Tpc44h[XO|YYm= NXq5b4VoSWO2aY\heIlwdiCxZjDld5Rzd2enbjDy[ZNxd26|ZTDlcIVu\W62IHnuJGhmVGFiY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iRYP0do9o\W5icnXj[ZB1d3JiYXzwbIEvNCCHQ{WwQVAvODByMENOwG0> M3TVeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOUC2NlgxLz5zMUmwOlI5ODxxYU6=
HeLa NIDQV3ZHfW6ldHnvckBie3OjeR?= Mme4RYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[mW2YT6sJGVEPTB;MD6wNFAxP87:TR?= NVjYdoJERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
SH-SY5Y MULGeY5kfGmxbjDhd5NigQ>? NWjQSZZNSWexbnnzeEBi[3Srdnn0fUBqdiC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjc4PhfUBqdiCVSD3TXVV[KG6ndYLvZoxie3SxbXGgZ4VtdHNiZYjwdoV{e2mwZzDFd5Rzd2enbjDy[YNmeHSxcjDi[ZRiNCCHQ{WwQVAvODByMd88US=> MmnUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HEK293 MnPRSpVv[3Srb36gZZN{[Xl? M333b3RRZ1SUQV7TVG9TXEWUOjDpcohq[mm2aX;uJI9nKE2SUDugeZB1[WunIDjNVHAsQiByLkK1JJVOMSCrbjDPR3Q{NWW6cILld5NqdmdiSFXLNlk{KGOnbHzzMEBKSzVyPUKuPFjPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjFzME[wO{c,OTJzMUC2NFc9N2F-
HEK293 NXf4VnNGTnWwY4Tpc44h[XO|YYm= MVHUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBOWFBtIIXweIFs\SBqTWDQL|ohOC5{NTD1UUkhcW5iT1PUNU1mgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczygTWM2OD13Lkez{txO NFPpOm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGxNFYxPyd-MUKxNVA3ODd:L3G+
MCF-7-2a NX3GRVZzTnWwY4Tpc44h[XO|YYm= Mm\uRYdwdmm|dDDhZ5Rqfmm2eTDpckB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBie3OjeTDpckBOS0ZvNz2yZUBk\WyuczDjc41x[XKnZDD0c{Bme3S{YXTpc4whTTJuIFXDOVA:OC5yMEC0Pe69VQ>? M3jybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUO5OFQ4Lz5zMkGzPVQ1PzxxYU6=
T47D MlTtSpVv[3Srb36gZZN{[Xl? NW\6bYZlSWexbnnzeEBm\m[nY4Sgc44hfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hcW5iVES3SEBk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijLDDFR|UxRTBwMECwNFfPxE1? MmPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2NUmwNVcoRjF{NEW5NFE4RC:jPh?=
T47D NXfOZ4hITnWwY4Tpc44h[XO|YYm= MWDB[49vcXO2IHXm[oVkfCCxbjD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDv[kBVPDeGIHPlcIx{KGW6cILld5NqdmdiaIXtZY4h\XO2cn;n[Y4hemWlZYD0c5Ih[mW2YTygSWM2OD1yLkCwNFjPxE1? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR3OUCxO{c,OTJ2NUmwNVc9N2F-
MCF-7 NXy1N3g{TnWwY4Tpc44h[XO|YYm= M3PlW3BmemOnboSgZYdwdmm|dHnjJIFkfGm4aYT5JIZweiCnc4Tyc4dmdi2rbnT1Z4VlKHCVMjDlfJBz\XO|aX;uJIlvKE2FRj23JINmdGy|LDDFR|UxRTBwMECwNFHPxE1? NFviUIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMke0PVg6QCd-MUK3OFk5QTh:L3G+
MCF-7 MmC0SpVv[3Srb36gZZN{[Xl? M{PpXmZ2dmO2aX;uZYwhTVKjbIDoZUBz\WOncITvdkBi[3Srdnn0fUBqdiC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjc4PhfUBqdiCPQ1[tO{Bk\WyuczygSWQ2OD1yLkCwNFAxPDcQvF2= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NEC0N{c,OTJ6MkSwOFM9N2F-
SAOS-2 M3u1OWZ2dmO2aX;uJIF{e2G7 MXrGeY5kfGmxbnHsJGV{fHKxZ3XuJJJm[2WydH;yJIJmfGFiYXP0bZZqfHliYomgcYV1[Wyub4TobY9v\WmwIHnu[JVkfGmxbjDpckBUSU:VLUKgZ4VtdHNuIFXEOVA:OC5yMES3{txO NV3MbYFVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4NlQxPDNpPkGyPFI1ODR|PD;hQi=>
MCF-7 breast tumor MX;GeY5kfGmxbjDhd5NigQ>? MY\F[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KE2FRj23JIJz\WG|dDD0eY1weiClZXzsd{B2e2mwZzDNR2YuPyCjc4PhfU4tKEWFNUC9NE4xODByMd88US=> NX;UOIpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4NlU6OzVpPkGyPFI2QTN3PD;hQi=>
MCF-7-2a NGjiNXNHfW6ldHnvckBie3OjeR?= M{[zXGNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZYN1cX[jdHWgcJVkcW[ncnHz[UBmgHC{ZYPzbY9vKGmwIF3DSk04NTKjIHPlcIx{NCCHQ{WwQVAvODByMElOwG0> M2rSPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2N{[xNVk{Lz5zNEe2NVE6OzxxYU6=
Ishikawa Var-1 cells MXTGeY5kfGmxbjDhd5NigQ>? NV3UdmdUPzJiaILz MlrYSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SCYYYKtNUBk\WyuczDhd5Nme3OnZDDhd{B{fGmvdXzheIlwdiCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBi[3Srdnn0fUBu\WG|dYLl[EBjgSCvZYThZo9tcXOvIH;mJJAudmm2cn;wbIVvd2xicHjvd5Bp[XSjc3WgZYZ1\XJiN{KgbJJ{NCCHQ{WwQVAvODByMES3{txO M3v0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NU[4O|cxLz5zNUW2PFc4ODxxYU6=
HeLa M{TuPGZ2dmO2aX;uJIF{e2G7 MVfJcohq[mm2aX;uJI9nKFt|SG3ld5Rz[WSrb3ygZolv\GmwZzD0c{BpfW2jbjDld5Rzd2enbjDy[YNmeHSxcjDi[ZRiKGW6cILld5Nm\CCrbjDI[WxiKGOnbHzzMEBKSzVyPUCuNFI1|ryP M2HzblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFUyLz5zNU[1PFg2OTxxYU6=
HeLa NYnrbGVCTnWwY4Tpc44h[XO|YYm= MVLJcohq[mm2aX;uJI9nKFt|SG3ld5Rz[WSrb3ygZolv\GmwZzD0c{BpfW2jbjDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[SCneIDy[ZN{\WRiaX6gTIVN[SClZXzsd{whUUN3ME2wMlAzQM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi1NUc,OTV4NUi4OVE9N2F-
COS-1 NFf2U5ZHfW6ldHnvckBie3OjeR?= MnzCTY4hfmm2cn:gZYdwdmm|dDDhZ5Rqfmm2eTDmc5Ih\XO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGg[ZhxemW|c3XkJIlvKEORUz2xJINmdGy|LDDFR|UxRTBwMECyOO69VQ>? NUHPN3FGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVM1ODdpPkG1O|E{PDB5PD;hQi=>
COS-1 NU[xO5djTnWwY4Tpc44h[XO|YYm= NX\4XVNzUW5idnn0do8h[WexbnnzeEBi[3Srdnn0fUBnd3JiZYP0do9o\W5icnXj[ZB1d3JiYnX0ZUBmgHC{ZYPz[YQhcW5iQ1;TMVEh[2WubIOsJGVEPTB;MD6wNFk4|ryP M1mzNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{GzOFA4Lz5zNUexN|QxPzxxYU6=
293T Ml2zSpVv[3Srb36gZZN{[Xl? NHrRZ3BFcXOybHHj[Y1mdnRib3[gX|NJZTF5YnX0ZU1me3S{YXTpc4wh\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDFVoFteGijIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwQVAvODByN{ZOwG0> NU[3dmM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNVk1PjNpPkG2NlE6PDZ|PD;hQi=>
293T M1fxTWZ2dmO2aX;uJIF{e2G7 NFPXcG1FcXOybHHj[Y1mdnRib3[gX|NJZTF5YnX0ZU1me3S{YXTpc4wh\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xODFzON88US=> NIfndYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxPVQ3Oyd-MU[yNVk1PjN:L3G+
HEK293 MVXGeY5kfGmxbjDhd5NigQ>? NWTGdVliTW[oZXP0JI9vKHS{YX7zZYN1cX[jdHnvckBw\iCDTGCg[4Vv\SCneIDy[ZN{cW:wIHnuJGhGUzJ7MzDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKGiHUnHsdIhiNCCHQ{WwQVAvODByN{ZOwG0> M3TzSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{C5PVA4Lz5zNkOwPVkxPzxxYU6=
HEK293 NFrpRZVHfW6ldHnvckBie3OjeR?= MojSSYZn\WO2IH;uJJRz[W6|YXP0bZZifGmxbjDv[kBCVFBiZ3Xu[UBmgHC{ZYPzbY9vKGmwIFjFT|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIhGWmKndHGsJGVEPTB;MD6wNFIy|ryP MkXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|MEm5NFcoRjF4M{C5PVA4RC:jPh?=
SK-BR-3 MVzGeY5kfGmxbjDhd5NigQ>? NXHHTIlGPDhiaILz Ml;qTY5pcWKrdHnvckBw\iClaHXtc4F1fHKjY4ThcpQucW6mdXPl[EBk\WyuIH3p[5JifGmxbjDv[kBpfW2jbjDTT{1DWi1|IHPlcIx{KGGodHXyJFQ5KGi{czDifUB1emGwc4flcIwhdWmpcnH0bY9vKGG|c3H5MEBKSzVyPUCuNFAxOc7:TR?= MnK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N|MoRjF4NUKwO|M{RC:jPh?=
COS7 MoHZSpVv[3Srb36gZZN{[Xl? NHPnbopFcXOybHHj[Y1mdnRib3[gSVIuSWyneHG2N|Mh\nKxbTDHSnAufGGpZ3XkJGVT[WyyaHGg[ZhxemW|c3XkJIlvKEORU{egZ4VtdHNiYomgSmFEWyxiS3m9NE4xODB|zszN M4nke|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwO|M{Lz5zNkWyNFc{OzxxYU6=
COS7 NYHvdGN7TnWwY4Tpc44h[XO|YYm= MXnB[49vcXO2IHHjeIl3cXS7IHH0JGdHWC22YXfn[YQhT1CUM{Cg[ZhxemW|c3XkJIlvKEORU{egZ4VtdHNiYYPz[ZN{\WRiYYOgbIFt\iCuaX\lJIZweiCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXygZpkhe3CnY4Tyc4ZtfW:{aX3leJJ6NCCHQ{WwQVAvODByM988US=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N|M9N2F-
COS7 MmLoSpVv[3Srb36gZZN{[Xl? NH\nNmZFcXOybHHj[Y1mdnRib3[gSVIuSWyneHG2N|Mh\nKxbTDHSnAufGGpZ3XkJGVT[mW2YTDlfJBz\XO|ZXSgbY4hS0:VNzDj[YxteyCkeTDGRWNUNCCNaU2wMlAxODN6zszN MlzFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N|MoRjF4NUKwO|M{RC:jPh?=
MCF7 NWHPSHRITnWwY4Tpc44h[XO|YYm= M{jNe|Q5KGi{cx?= NXX5WZl1UW6qaXLpeIlwdiCxZjDjbIVud2G2dILhZ5RidnRvaX7keYNm\CClZXzsJI1q\3KjdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUB1emGwc4flcIwhdWmpcnH0bY9vKGG|c3H5MEBKSzVyPUCuNFAxPM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N|M9N2F-
COS7 MVjGeY5kfGmxbjDhd5NigQ>? MVnEbZNxdGGlZX3lcpQhd2ZiRUKtRYxmgGF4M{Og[pJwdSCJRmCteIFo\2WmIFfQVlMxKGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIJ6KE[DQ2OsJGtqRTBwMEC1O:69VQ>? Mn:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N|MoRjF4NUKwO|M{RC:jPh?=
COS7 MkCwSpVv[3Srb36gZZN{[Xl? MorONUBvVQ>? NUXCTXZzSWexbnnzeEBi[3Srdnn0fUBifCCJRmCteIFo\2WmIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbY51emGlZXzseYxieiClYXzjbZVuKGyndnXsJIF1KDFibl2gZpkhe3CnY4Tyc4ZtfW:{aX3leJJ6 NH\WVZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWyNFc{Oyd-MU[1NlA4OzN:L3G+
COS7 M2PJbWZ2dmO2aX;uJIF{e2G7 MUOxJI5O M2\yZWFod26rc4SgZYN1cX[rdImgZZQhT0[SLYTh[4dm\CCHUnLleIEh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBk[WylaYXtJIxmfmWuIHH0JFEhdk1iYomgd5Bm[3S{b3\seY9zcW2ndIL5 M4nvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwO|M{Lz5zNkWyNFc{OzxxYU6=
COS7 NXXyN5dtTnWwY4Tpc44h[XO|YYm= MVGxJI5O MVzB[49vcXO2IHHjeIl3cXS7IHH0JGdHWC22YXfn[YQhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczDhd5Nme3OnZDDhd{BRUTONIHHjeIl3[XSrb36gZZQhOSCwTTDifUBRUVB|IH71Z4xm[XJiYXPjeY12dGG2aX;uJIF{e2G7 MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N|M9N2F-
COS7 MYLGeY5kfGmxbjDhd5NigQ>? MYSxJJVO MkTVRYdwdmm|dDDhZ5Rqfmm2eTDheEBITlBvdHHn[4VlKEWUYnX0ZUBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczDhd5Nme3OnZDDhd{BRUTONIHHjeIl3[XSrb36gZZQhOSCwTTDifUBRUVB|IH71Z4xm[XJiYXPjeY12dGG2aX;uJIF{e2G7 MoHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N|MoRjF4NUKwO|M{RC:jPh?=
COS7 MmW0SpVv[3Srb36gZZN{[Xl? NXrxZpdDOSC3TR?= NXToXpBbSWexbnnzeEBi[3Srdnn0fUBifCCJRmCteIFo\2WmIFfQVlMxKGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIF{e2W|c3XkJIF{KFCLM1ugZYN1cX[jdHnvckBifCBzIH7NJIJ6KFCLUEOgcpVkdGWjcjDhZ4N2dXWuYYTpc44h[XO|YYm= Mn7oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N|MoRjF4NUKwO|M{RC:jPh?=
COS7 NHvLOFVHfW6ldHnvckBie3OjeR?= NVy3TpQ1OSCwTR?= M33BVWJqdmSrbnegZYZncW6rdImgeI8hdVKIUEGteIFo\2WmIFfQVlMxKGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJRmCteIFo\2WmIFXSZYxxcGFiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCHMj3BcIV5[TZ|MzDibY5lcW6pIHnuJI52[2yndYOgZZQhOSC3TTDifUBHSUOV MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N|M9N2F-
COS7 MUHGeY5kfGmxbjDhd5NigQ>? M1XKSlEhdk1? MmPHRolv\GmwZzDh[oZqdmm2eTD0c{BITlBvdHHn[4VlKEWUYXzwbIEh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZ49mgHC{ZYPzbY5oKG2URmCxMZRi\2enZDDHVHI{OCCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEV{LVHs[ZhiPjN|IHLpcoRqdmdiaX6gcpVkdGW3czDheEAyKHWPIHL5JGZCS1N? M{[0TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwO|M{Lz5zNkWyNFc{OzxxYU6=
MCF7 NF:0SWJHfW6ldHnvckBie3OjeR?= M33MZ|Ehdk1? NXLZXllLOjRiaILz MlvRSI94diC{ZXf1cIF1cW:wIH;mJIV{fHKxZ3XuJJJm[2WydH;yJIV5eHKnc4Ppc44hcW5iTVPGO{Bk\WyuczDheEAyKG6PIHHmeIVzKDJ2IHjydy=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ5NUOxOUc,OTd{N{WzNVU9N2F-
HEK293 NIHuSnFHfW6ldHnvckBie3OjeR?= NF7CZWNC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEWUYXzwbIEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{BjgSC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTB;MD6wNFA4Pc7:TR?= MnzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NEi2OVYoRjF5NES4OlU3RC:jPh?=
HEK293 M1;GTmZ2dmO2aX;uJIF{e2G7 MX\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JGVT[mW2YTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHL5JJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NF0xNjByMkJOwG0> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR2OE[1Okc,OTd2NEi2OVY9N2F-
HEK293 NWXZbVRpTnWwY4Tpc44h[XO|YYm= MUTB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVA:OC5yMEC3Oe69VQ>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7MEC4OEc,OTd6OUCwPFQ9N2F-
HEK293 MkPtSpVv[3Srb36gZZN{[Xl? NGLCe3BC\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OD1yLkCwNlHPxE1? NF\2W409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i5NFA5PCd-MUe4PVAxQDR:L3G+
MCF7 BUS NYrMSIc6TnWwY4Tpc44h[XO|YYm= MVnFd5Rzd2enbnnjJJBwfGWwY4mgbY4hcHWvYX6gUWNHPyCEVWOgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBqdmS3Y3nu[{Bu[XirbXHsJINmdGxicILvcIln\XKjdHnvckwhVUWGPUCuNFAy|ryP M2PTcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkSyPVk4Lz5zOEK0Nlk6PzxxYU6=
CHO NWPNO2diTnWwY4Tpc44h[XO|YYm= M2O4N2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gWGdTPSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KGy3Y3nm[ZJie2ViYYPzZZktKEWFNUC9N|gvPM7:TR?= MkDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|MEeyPVQoRjF6M{C3Nlk1RC:jPh?=
MCF7 NHXOVFNHfW6ldHnvckBie3OjeR?= NFvZe5k3KGSjeYO= NYO3ZpNUTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJINmdGxicILvcIln\XKjdHnvckBi\nSncjC2JIRigXNiYomgW3NVQCCjc4PhfUwhTUN3ME2wMlAxODByM{hOwG0> Ml7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5NkC2NFMoRjF6N{[wOlA{RC:jPh?=
HEC1 NXroboNXTnWwY4Tpc44h[XO|YYm= MYKyOEBpenN? NUXEOYpQSWexbnnzeEBi[3Srdnn0fUBifCCodXzsMYxmdme2aDDoeY1idiCnc4Tyc4dmdiC{ZXPldJRweiCkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUNzIHPlcIx{KGG|c3Xzd4VlKGG|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFAyO87:TR?= M4r5d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK4NFE3Lz5zOUGyPFAyPjxxYU6=
HEC1 Mmi2SpVv[3Srb36gZZN{[Xl? NILpSGQzPCCqcoO= M3rOcWFod26rc4SgZYN1cX[rdImgZZQh\nWubD3s[Y5ofGhiaIXtZY4h\XO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGg[ZhxemW|c3XkJIlvKGi3bXHuJGhGSzFiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwNFfPxE1? NGHqWW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGyPFAyPid-MUmxNlgxOTZ:L3G+
HEC1 Mne4SpVv[3Srb36gZZN{[Xl? NEH3cpozPCCqcoO= NVnwRVhKSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGW6cILld5Nm\CCrbjDoeY1idiCKRVOxJINmdGy|IHHzd4V{e2WmIHHzJJRz[W6|Y4LpdJRqd26jbDDwc5RmdmO7IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnLXLleIEh\2GuYXP0c5Nq\GG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFAxOc7:TR?= M16wcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7Mki2Nlg{Lz5zOUK4OlI5OzxxYU6=
HEC1 NXm2Z2NlTnWwY4Tpc44h[XO|YYm= NETNUFYzPCCqcoO= MonYRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWmKndHGg[ZhxemW|c3XkJIlvKGi3bXHuJGhGSzFiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKHCxdHXuZ5kh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4Uu[mW2YTDnZYxi[3Sxc3nkZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEC1{txO NHjs[GM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK4OlI5Oyd-MUmyPFYzQDN:L3G+
BL21(DE3) NULIbYlyTnWwY4Tpc44h[XO|YYm= M4HFTGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUhFTTNrIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBkd2GldHn2ZZRweiB3Mz3wdo91\WmwIHLpcoRqdmdiYomg[ox2d3Knc3PlcoNmKGG|c3H5 NVG2OlhtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OlE3OTlpPkG5OVYyPjF7PD;hQi=>
BL21(DE3) NH;4U2xHfW6ldHnvckBie3OjeR?= MYLB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZoV1[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW|c3XkJIlvKEW|Y3jldolkcGmjIHPvcIkhSkx{MTjESVMqKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDjc4FkfGm4YYTvdkA2Oy2ycn;0[YlvKGKrbnTpcoch[nliZnz1c5Jme2OnbnPlJIF{e2G7 NGftXoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW2NVYyQSd-MUm1OlE3OTl:L3G+
SF9 MkjkSpVv[3Srb36gZZN{[Xl? MWPEbZNxdGGlZX3lcpQhd2ZiW{PIYVE4[mW2YT3ld5Rz[WSrb3yg[pJwdSCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCVRkmgZ4VtdHNuIFnDOVA:OC5yMUK1{txO M2H5cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEO2PVQ6Lz5zOUizOlk1QTxxYU6=
COS7 MlTmSpVv[3Srb36gZZN{[Xl? MVzB[49vcXO2IHHjeIl3cXS7IHH0JIV{fHKxZ3XuJJJm[2WydH;yJIFteGijIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStUGJFKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxOzMQvF2= MlnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NkOwPFMoRjF7OE[zNFg{RC:jPh?=
COS7 Mn7MSpVv[3Srb36gZZN{[Xl? MVHB[49vcXO2IHHjeIl3cXS7IHH0JIV{fHKxZ3XuJJJm[2WydH;yJIJmfGFibHnnZY5lKGKrbnTpcoch\G:vYXnuJIV5eHKnc4Pl[EBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDU3M4KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIghT2GuND3MRmQh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFEy|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh4M{C4N{c,OTl6NkOwPFM9N2F-
MCF7:WS8 NISyS4RHfW6ldHnvckBie3OjeR?= NIHzbGI4KGSjeYO= M4jIVGV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hTVKjbIDoZUBmgHC{ZYPzbY5oKE2FRke6W3M5KGOnbHzzJIF{e2W|c3XkJIF{KHO2aX31cIF1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBi\nSncjC3JIRigXNuIFXDOVA:OC5yMECwNFHPxE1? MnzUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|M{SzOlgoRjJyM{O0N|Y5RC:jPh?=
MCF7:WS8 MlPrSpVv[3Srb36gZZN{[Xl? MYTBZ5RqfmG2aX;uJI9nKGi3bXHuJGVTKGmwIHj1cYFvKE2FRke6W3M5KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemW|cH;ud4l3\SCnbHXt[Y51KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MkLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|M{SzOlgoRjJyM{O0N|Y5RC:jPh?=
T47D:C:4:2 MXXGeY5kfGmxbjDhd5NigQ>? M4jsZWV{fHKxZ3XubYMh[WO2aY\peJkh[XRid3ns[EB1gXCnIFXSJIFteGijIHX4dJJme3OnZDDpckBVPDeGOlO6OFozKGOnbHzzJINw\XiycnXzd4lv\yCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGK7IHT1[YwhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7 Ml7KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|M{SzOlgoRjJyM{O0N|Y5RC:jPh?=
T47D:C:4:2 NEfkVFlHfW6ldHnvckBie3OjeR?= NXPtS|dlTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDD3bYxlKHS7cHWgSXIh[WyyaHGgSFM2OUdibYX0ZY51KGW6cILld5Nm\CCrbjDUOFdFQkN8NEqyJINmdGy|IHPv[ZhxemW|c3nu[{Bme3S{b3flckBz\XOyb37z[UBmdGWvZX70JIJ6KGS3ZXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN|NEO2PEc,OjB|M{SzOlg9N2F-
HEK293 NWn3dpJnTnWwY4Tpc44h[XO|YYm= MoDJNVghcHK| Moe0WJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGGodHXyJFE5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOlE4|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyOEWzNkc,OjB2MEi1N|I9N2F-
HEK293 M3nJXmZ2dmO2aX;uJIF{e2G7 NIfGd2cyQCCqcoO= M4POTHRz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDFVoFteGijIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwNFA3PDcQvF2= NXjnZ2Y6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFg2OzJpPkKwOFA5PTN{PD;hQi=>
CHO-K1 NVTWSIk2TnWwY4Tpc44h[XO|YYm= NVj0[IVSSWexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEh\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxRTBwMEROwG0> MoTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3NUOwNlMoRjJyNUWzNFI{RC:jPh?=
CHO-K1 MV;GeY5kfGmxbjDhd5NigQ>? NIizc|lC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEh\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxRTBwMEZOwG0> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV3M{CyN{c,OjB3NUOwNlM9N2F-
HeLa NX7JfoVMTnWwY4Tpc44h[XO|YYm= M{D4UlE5KGi{cx?= MWTEbZNxdGGlZX3lcpQhd2ZiW{KsOEw3NDdvM1jd[ZN1emGmaX;sJIZzd21iaIXtZY4hTVKjbIDoZUBmgHC{ZYPz[YQhcW5iSHXMZUBk\WyuczDh[pRmeiBzODDodpMh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwMECwNVbPxE1? NVPLNXEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NlE1QTJpPkKwOlIyPDl{PD;hQi=>
HEK293T MXXGeY5kfGmxbjDhd5NigQ>? M1zhOWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDFN|U{SSCvdYThcpQh\XiycnXzd4VlKGmwIFjFT|I6O1RiY3XscJMh[29vZYjwdoV{e2mwZzDFVmUh[XO|ZYPz[YQh[XNidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAxOjYQvF2= M2K4Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkW5PFAyLz5{ME[1PVgxOTxxYU6=
HEK293T NEHGe2dHfW6ldHnvckBie3OjeR?= MX3B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZoV1[SCHM{C1RUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N3Qh[2WubIOgZ48u\XiycnXzd4lv\yCHUlWgZZN{\XO|ZXSgZZMhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCxNFTPxE1? NWTiW|loRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2OVk5ODFpPkKwOlU6QDBzPD;hQi=>
HeLa NWLqdVZkTnWwY4Tpc44h[XO|YYm= NHTDdmVC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJdqdGRidInw[UBGWmGucHjhJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLXX4dJJme3OrbnegSXJGKGG|c3Xzd4VlKGG|IITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NGXrZZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[1PVgxOSd-MkC2OVk5ODF:L3G+
HeLa MkezSpVv[3Srb36gZZN{[Xl? M2LjTnRz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjD3bYxlKHS7cHWgSXJidHCqYTDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy2neIDy[ZN{cW6pIFHQMVEh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 NGH5c5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[1PVgxOSd-MkC2OVk5ODF:L3G+
HeLa NIXMTW1HfW6ldHnvckBie3OjeR?= NX:yVY1VSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckB4cWymIIT5dIUhTVKkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGOxLXX4dJJme3OrbnegSXJGKGG|c3Xzd4VlKGG|IITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> M2TKVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkW5PFAyLz5{ME[1PVgxOTxxYU6=
HeLa NIXFVo5HfW6ldHnvckBie3OjeR?= NHrzTZNCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKHerbHSgeJlx\SCHUnLleIEh\XiycnXzd4VlKGmwIHj1cYFvKEinTHGgZ4VtdHNiY3:t[ZhxemW|c3nu[{BCWC1zIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> Mmj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4NUm4NFEoRjJyNkW5PFAyRC:jPh?=
U2OS M2LOXWZ2dmO2aX;uJIF{e2G7 MoXVNVghcHK| M17HcWFod26rc4SgZYN1cX[rdImgZZQhTVKkZYThMWxDTCCrbjDoeY1idiCXMl;TJINmdGy|IITyZY5{\mWldHXkJJdqfGhiR3HsOE1FSkRiYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4YhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIFnfGW{IEG4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODB2NUix{txO M4T4e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEGyOlgyLz5{MEixNlY5OTxxYU6=
U2OS M2LYPGZ2dmO2aX;uJIF{e2G7 M3Ty[FE5KGi{cx?= NFvYOmpC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEuVEKGIHnuJIh2dWGwIGWyU3Mh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDHZYw1NUSERDDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBw\iC2cnHud4FkfGm4YYTpc44h[WO2aY\peJkh[W[2ZYKgNVghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMke3N:69VQ>? NGnH[mg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEixNlY5OSd-MkC4NVI3QDF:L3G+
U2OS NVrxbXJjTnWwY4Tpc44h[XO|YYm= NH\1TlQyQCCqcoO= M{TOPWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiRWLi[ZRiNUyERDDpckBpfW2jbjDVNm9UKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigS4FtPC2GQlSgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD0doFve2GldHn2ZZRqd25iYXP0bZZqfHliYX\0[ZIhOThiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkCxNFc1|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODhzMk[4NUc,OjB6MUK2PFE9N2F-
U2OS NGjrUJpHfW6ldHnvckBie3OjeR?= MVqxPEBpenN? NGPjNm5CdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFvTFLEJIlvKGi3bXHuJHUzV1NiY3XscJMhfHKjboPm[YN1\WRid3n0bEBI[Wx2LVTCSEBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJRz[W6|YXP0bZZifGmxbjDhZ5Rqfmm2eTDh[pRmeiBzODDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNFIyODUQvF2= NV3FNYxTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4NVI3QDFpPkKwPFEzPjhzPD;hQi=>
HEK293 MkXLSpVv[3Srb36gZZN{[Xl? M3PkcmFod26rc4SgZYN1cX[rdImgZZQhYk[SLX\1d4VlKEWUUnLleIEhVEKGIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgWHIuTlKHVDDhd5NigSxiRVO1NF0xNjByNEdOwG0> M2PhXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkG4O|g{Lz5{MUKxPFc5OzxxYU6=
MCF7 NVvsbpA6TnWwY4Tpc44h[XO|YYm= MlzSeZAhfG9iNjDkZZl{ NV\zZnhCSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJGxDTCCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhfXBidH:gOkBl[Xm|IHL5JINmdGy2aYTldk1odG9iYYPzZZktKEWFNUC9NE4xOc7:TR?= NFLlTmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUKxPFc5Oyd-MkGyNVg4QDN:L3G+
MCF7 MUHDfZRwfG:6aXPpeJkh[XO|YYm= NXnqVZVRPCC3TR?= MXSyOEBpenN? M17mR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XRiNDD1UUBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5 NYO5XHdzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OVk2QDFpPkKxOFU6PThzPD;hQi=>
MCF7 Mo\hSpVv[3Srb36gZZN{[Xl? M3X0dFQhfU1? MY[yOEBpenN? M2jQb3Jm\HWldHnvckBqdiCFZHuxJJBzd3SnaX6gcIV3\WxiaX6gbJVu[W5iTVPGO{Bk\WyuczDheEA1KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NIDDUGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS1PVU5OSd-MkG0OVk2QDF:L3G+
HEC1 NU\zOYxbTnWwY4Tpc44h[XO|YYm= NH7BdJczPCCqcoO= NVXBXJpjSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBnfWyuLXzlcod1cCCHUnHsdIhiKHKnY3XweI9zKGW6cILld5Nm\CCrbjDoeY1idiCKRVOxJINmdGy|IHPvMYV5eHKnc4PpcochMEWURTmyMZBUOi2udXOg[4Vv\SCjc4Pld5Nm\CCjczD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUCuNFAxODoQvF2= M{jPWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEixOFk4Lz5{MUS4NVQ6PzxxYU6=
HEC1 NWDsfINOTnWwY4Tpc44h[XO|YYm= NWnQfWlCOjRiaILz M{\LPGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6g[pVtdC2uZX7neIghTVKkZYThJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUNzIHPlcIx{KGOxLXX4dJJme3OrbnegLGVTTSl{LYDTNk1tfWNiZ3Xu[UBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwO|LPxE1? NG\3bIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS4NVQ6Pyd-MkG0PFE1QTd:L3G+
MCF7 M3zLNWZ2dmO2aX;uJIF{e2G7 M3XGZ|AvODFibl2= NF3U[Yc1QCCqcoO= M3HwdGV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hTVJvcH;zbZRqfmViTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{B{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCjY4Tpeol1gSCjdDCwMlAyKG6PIHHmeIVzKDR6IHjyd{BjgSCyaH;zdIhiNWyrZ3j0JJJmeG:{dHXyJINp\W2rbIXtbY5me2OnbnPlJIF{e2G7 M{LacFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOECwPFU6Lz5{MUiwNFg2QTxxYU6=
HEK NHfONmRHfW6ldHnvckBie3OjeR?= NUSyVWdtOThiaILz NFzOW3RC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEh\XiycnXzd4VlKGmwIFjFT{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGKndHGtcIFkfGGvYYPlJIFnfGW{IEG4JIhzeyCkeTDi[ZRiNWyjY4ThcYF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNFQ3|ryP MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh6NUK3PUc,OjF6OEWyO|k9N2F-
UAS M1fxPGZ2dmO2aX;uJIF{e2G7 NFm0TYwyQCCqcoO= NHPKepdC\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBmgHC{ZYPz[YQhcW5iVVHTJINmdGy|IHPvMYV5eHKnc4Ppcoch[mW2YT3sZYN1[W2jc3WgZYZ1\XJiMUigbJJ{KGK7IHLleIEudGGldHHtZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECyOe69VQ>? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh6NUK3PUc,OjF6OEWyO|k9N2F-
HEC-1 MUDGeY5kfGmxbjDhd5NigQ>? M13kTlI1KGi{cx?= MVHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhGSy1zIHPlcIx{KGOxZYjwdoV{e2mwZzDi[ZRiNWejbDDhd5Nme3OnZDDhd{BtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMUKz{txO MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
U2OS NXj1co9rTnWwY4Tpc44h[XO|YYm= MojHNlQhcHK| MVTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJHUzV1NiY3XscJMh[2:neIDy[ZN{cW6pIIDDUXgucFOUQ{OgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMEG1{txO NYjwb4ZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ|PD;hQi=>
HEC-1 NVjYRnRrTnWwY4Tpc44h[XO|YYm= NXzP[G5MOjRiaILz NGexNI9C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[mW2YTDlfJBz\XO|ZXSgbY4hUEWFLUGgZ4VtdHNiY3;lfJBz\XO|aX7nJIJmfGFvZ3HsJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECy{txO M4XlXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
U2OS NX3PNWw{TnWwY4Tpc44h[XO|YYm= NELPVGkzPCCqcoO= MXTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gWVJQWyClZXzsd{Bkd2W6cILld5NqdmdicFPNXE1pW1KFMzDhd5Nme3OnZDDhd{BtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByNN88US=> NGroT4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGyNlU3Oyd-MkKxNlI2PjN:L3G+
BL21 MVvGeY5kfGmxbjDhd5NigQ>? M3rxO|EhcHJ? M{DSe2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKE6USVStV3JEOyCxZjDFVoFteGijIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy|IHHmeIVzKDFiaIKgZpkhXFJvRmLFWEBie3OjeTygSWM2OD1yLkCwNFU3|ryP M{TmOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
BL21 NGDEOWxHfW6ldHnvckBie3OjeR?= Mnq1NUBpeg>? Mm\pRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDOMWhqezZvdHHn[4VlKHSncnLpeY0udGGkZXzs[YQhVlKLRD3TVmM{KG:oIFXSZoV1[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW|c3XkJIlvKEW|Y3jldolkcGmjIHPvcIkhSkx{MTDj[YxteyCjZoTldkAyKGi{IHL5JHRTNU[URWSgZZN{[XluIFXDOVA:OC5yMEC5{txO M33SNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
BL21 NGDRdm9HfW6ldHnvckBie3OjeR?= Mme4NUBpeg>? NUXCO49NSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBPNUirc{[teIFo\2WmIITldoJqfW1vbHHi[Yxt\WRiRWLi[ZRiKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIHPlcIx{KGG|c3Xzd4VlKGG|IILlZ5J2cXSvZX70JI9nKG[udX;y[ZNk\WmwLXzhZoVtdGWmIGPSR|Mh[W[2ZYKgNUBpeiCkeTDUVk1HWkWWIHHzd4F6NCCHQ{WwQVAvODBzNd88US=> MnzmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
BL21 M4TPcGZ2dmO2aX;uJIF{e2G7 NUXWNmt{OSCqch?= NEm1W2pC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJG4uUGm|Nj30ZYdo\WRidHXyZol2dS2uYXLlcIxm\CCHUnHsdIhiKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIHPlcIx{KGG|c3Xzd4VlKGG|IILlZ5J2cXSvZX70JI9nKG[udX;y[ZNk\WmwLXzhZoVtdGWmIGPSR|Mh[W[2ZYKgNUBpeiCkeTDUVk1HWkWWIHHzd4F6NCCHQ{WwQVAvODB|zszN M4LUPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
Ishikawa cells NVz2UFhOTnWwY4Tpc44h[XO|YYm= NH\neFQ{KGSjeYO= MXfB[49vcXO2IHHjeIl3cXS7IHH0JIV{fHKxZ3XuJJJm[2WydH;yJIlvKGi3bXHuJGl{cGmtYYfhJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGGua3HsbY5mKHCqb4PwbIF1[XOnIHzleoVtKGGodHXyJFMh\GG7czD1d4lv\yCSLX7peJJweGinbonsJJBpd3OyaHH0[UBieyC|dXLzeJJifGVuIFXDOVA:OC5yMEC5{txO MnfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzN{i1OVIoRjJ{MUe4OVUzRC:jPh?=
HepG2 NHvk[JRHfW6ldHnvckBie3OjeR?= MV[yOEBpenN? MU\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEKy{txO M4K4dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN|I5Lz5{MkK4N|MzQDxxYU6=
HepG2 M2HSXWZ2dmO2aX;uJIF{e2G7 NWr3VFJjOjRiaILz M3nufWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJj\XSjIHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMEGx{txO Mm\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OEOzNlgoRjJ{MkizN|I5RC:jPh?=
MCF7 NVTJUlZ7SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NWDjTIZrPzJiaILz MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwLXTl[olkcWWwdDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:OC5yMECwNFY1|ryP M4jiPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[0OlgyLz5{MkS2OFY5OTxxYU6=
MCF7:D5L NELY[YtHfW6ldHnvckBie3OjeR?= M4jaRVE3KGi{cx?= MWjFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KGW|dILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKE2FRke6SFVNKGOnbHzzJINwNWW6cILld5NqdmdiRWLFJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDseYNq\mW{YYPlJIdmdmViZYjwdoV{e2mxbjDh[pRmeiBzNjDodpMh[nlibXnjdo9xdGG2ZTDy[YFl\XJuIFXDOVA:OC5yMECwNVk3|ryP NWC4NZd[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
Ishikawa cells M{PxXGZ2dmO2aX;uJIF{e2G7 MYi3NkBpenN? MWXFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KGW|dILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKEm|aHnrZZdiKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcItidGmwZTDwbI9{eGijdHHz[UBmgHC{ZYPzbY9vKGGodHXyJFczKGi{czDifUBucWO{b4DsZZRmKHKnYXTldkwhTUN3ME2wMlAxODB{NEdOwG0> Ml7FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkS2PFEoRjJ{NE[0OlgyRC:jPh?=
Ishikawa cells NVHWS49zTnWwY4Tpc44h[XO|YYm= NFHEemoxNjFibl2= NUH1epFJPzJiaILz MlqzSYZn\WO2IH;uJINmdGxicILvcIln\XKjdHnvckBw\iCqdX3hckBKe2ira3H3ZUBk\WyuczDheEAxNjFibl2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?= NYrGU5FkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
CHO-K1 NUPySZVDTnWwY4Tpc44h[XO|YYm= NHvuO|QzPCCqcoO= NWrOcGdZXHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hd2ZiRWLi[ZRiKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMECzOu69VQ>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
CHO-K1 NXzCPJhMTnWwY4Tpc44h[XO|YYm= MUSyOEBpenN? NYDGN3NHXHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hd2ZiRWLhcJBp[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOe69VQ>? MoTjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109 MUHGeY5kfGmxbjDhd5NigQ>? M{X3WFI1KGi{cx?= NFXBd2NC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhVEKGIHX4dJJme3OnZDDpckB6\WG|dDDBTFExQSClZXzsd{Bie3Onc4Pl[EBieyClb33wc5Vv\CClb37j[Y51emG2aX;uJIF1KHeqaXPoJFExLSCjZ3;ubZN1KGGldHn2bZR6KG:oIITo[UBu[XirbYXtJIFkfGm4aYT5JI9nKEV{IHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCDY4Tpeol1gT1yLkCwNFHPxE1? MnvxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109 M1q0XWZ2dmO2aX;uJIF{e2G7 MlrtNlQhcHK| M1rUZ2Fod26rc4SgZYN1cX[rdImgZZQhTVKkZYThJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMh[2:vcH;1coQh[2:wY3XueJJifGmxbjDheEB4cGmlaDCxNEUh[WexbnnzeEBi[3Srdnn0fUBw\iC2aHWgcYF5cW23bTDhZ5Rqfmm2eTDv[kBGOiCjZoTldkAzPCCqcoOgZpkh[WyyaHGt[4Ft[WO2b4Pp[IF{\SCjc4PhfUwhSWO2aY\peJk:OC5yMECxPO69VQ>? NIrzPXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O|IyPyd-MkK2OFczOTd:L3G+
yeast AH109 MkXVSpVv[3Srb36gZZN{[Xl? MoHpNlQhcHK| MUTB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGgdoVk\XC2b4KgUGJFKGW6cILld5Nm\CCrbjD5[YF{fCCDSEGwPUBk\WyuczDhd5Nme3OnZDDhd{BqdnSncnHjeIlwdiC5aYToJHNTSzFiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEWFNUC9NE4xODFyNd88US=> MljNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109 M13QdGZ2dmO2aX;uJIF{e2G7 M1PpT|I1KGi{cx?= NEHxUnRC\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W|c3XkJIF{KGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiRVO1NF0xNjByMUC4{txO MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
U2-OS NX;xcmMxTnWwY4Tpc44h[XO|YYm= MortRYN1cX[jdHnvckBw\iCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCqdX3hckBWOi2RUzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZkhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2xuIFXDOVA:OC5yMECwNFTPxE1? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
U2-OS NITP[WZHfW6ldHnvckBie3OjeR?= NF;wXFJC[3SrdnH0bY9vKG:oIHj1cYFvKEWUYnX0ZUBmgHC{ZYPz[YQhcW5iaIXtZY4hXTJvT2OgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:uLDDFR|UxRTBwMECwNFHPxE1? NELrTJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C0N|I1Oid-MkOwOFMzPDJ:L3G+
MCF7/2a M3nydWZ2dmO2aX;uJIF{e2G7 NXfvb3RUSWO2aY\heIlwdiCxZjDFVoFteGijIHnuJIh2dWGwIF3DSlcwOmFiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5JJJmdGG2aY\lJJRwKHWwdILlZZRm\CClb370do9tNCCHQ{WwQVAvODByMd88US=> M3jjSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MESzNlQzLz5{M{C0N|I1OjxxYU6=
MCF7 MkiySpVv[3Srb36gZZN{[Xl? MkjQNUB2VQ>? MkTDSI94diC{ZXf1cIF1cW:wIH;mJGVT[WyyaHGgbJVu[W5iTVPGO{Bk\WyuczDheEAyKHWPIHL5JHdme3Sncn6gZoxwfHSrbne= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
HeLa NX;qOZlCTnWwY4Tpc44h[XO|YYm= NFv4[lgyKG6P NHPRT2kzOCC2bzCyOEBpenN? NX62OFhLSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKHKnY3XweI9zKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iCHUnHsdIhiNWKjc3XkJJRz[W6|Y4LpdJRqd25iYYSgNUBvVSCjZoTldkAzOCC2bzCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 Ml7kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEizOFYoRjJ|NES4N|Q3RC:jPh?=
MCF7 MmPaSpVv[3Srb36gZZN{[Xl? MlTaNUBvVQ>? NXjpeoF{OjBidH:gNlQhcHK| M2fEcWFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBz\WOncITvdkBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4YhTVKjbIDoZU1j[XOnZDD0doFve2O{aYD0bY9vKGG2IEGgcm0h[W[2ZYKgNlAhfG9iMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NIP5S|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0PFM1Pid-MkO0OFg{PDZ:L3G+
MCF7:WS8 MV3GeY5kfGmxbjDhd5NigQ>? M2GwSlQ5KGi{cx?= Mn;PRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWiCrbjDoeY1idiCPQ1[3PndUQCClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDwV|Ih\2WwZTDlfJBz\XO|aX;uJIF1KDFyJz2xNEBOKGGodHXyJFQ5KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= MmPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEWxPVkoRjJ2OEC1NVk6RC:jPh?=
MCF7:WS8 M4TKVWZ2dmO2aX;uJIF{e2G7 M1LSVlQ5KGi{cx?= NV\mN4pKSWexbnnzeEBi[3Srdnn0fUBifCCHUjDpckBpfW2jbjDNR2Y4QleVODDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBIWkWEMTDn[Y5mKGW6cILld5Nqd25iYYSgNVAoNTFyIF2gZYZ1\XJiNEigbJJ{KGK7IGLUMXBEWiCjbnHsfZNqew>? NYDrenV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NFUyQTlpPkK0PFA2OTl7PD;hQi=>
MCF7:WS8 M1vqbGZ2dmO2aX;uJIF{e2G7 NGe2cWY1QCCqcoO= NHvCT4RC\2:waYP0JIFkfGm4aYT5JIF1KEWUIHnuJIh2dWGwIF3DSlc7X1N6IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJHBoWiCpZX7lJIV5eHKnc4Ppc44h[XRiMUCnMVExKE1iYX\0[ZIhPDhiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> NH7ZcG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEiwOVE6QSd-MkS4NFUyQTl:L3G+
MCF7 NF3weWNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1mzWlIh\GG7cx?= MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;MkeuN|XPxE1? NI\rU|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKyNlg4Pid-MkWyNlI5PzZ:L3G+
MDA-MB-231 NVO1cWlsSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NU\TN5J5OiCmYYnz NITQcVNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbjDFVk1v\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;M{OuOFbPxE1? Mn3jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MkK4O|YoRjJ3MkKyPFc3RC:jPh?=
B16F10 M2DwNGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEG5fVczKGSjeYO= MmXNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgcY92e2ViQkG2SlExKGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;M{muPFHPxE1? NWXxU|ZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNlI5PzZpPkK1NlIzQDd4PD;hQi=>
MIAPaCa2 M33FOWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWrq[IpkOiCmYYnz MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idiCPSVHQZWNiOiClZXzsd{Bi\nSncjCyJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUSyMlk{|ryP NGK2b409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKyNlg4Pid-MkWyNlI5PzZ:L3G+
HEK293 MojhSpVv[3Srb36gZZN{[Xl? NIGxN3pC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwNlTPxE1? NYnIepZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFU3QDhpPkK1N|A2Pjh6PD;hQi=>
HEK293 MmG5SpVv[3Srb36gZZN{[Xl? Mlv1RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODByOEZOwG0> NXvKPZhsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFU3QDhpPkK1N|A2Pjh6PD;hQi=>
MCF7 MoniSpVv[3Srb36gZZN{[Xl? M{PoT|I1KGi{cx?= NFLYWGtC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCycn;n[ZN1\XKxbnWgdoVk\XC2b4Kg[Y5ld2enbn;1d{Bo\W6nczDhZ5RqfmG2aX;uJIFnfGW{IEK0JIhzeyCkeTDxVGNTNCCHQ{WwQVAvODByMEC4OO69VQ>? NX\5XFVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVkzOTNpPkK1OVU6OjF|PD;hQi=>
HEC1 MXvGeY5kfGmxbjDhd5NigQ>? M2PKN|I1KGi{cx?= MlHFRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoFteGijIHX4dJJme3OnZDDpckBpfW2jbjDISWMyKGOnbHzzJIF{e2W|c3XkJIF{KHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGGodHXyJFI1KGi{czDifUBGWkVvbIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKHS{YX7z[oVkfGmxbjDhd5NigSxiRVO1NF0xNjByMEG2{txO NEW1S209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1PVIyOyd-MkW1OVkzOTN:L3G+
MCF7 MWTGeY5kfGmxbjDhd5NigQ>? NIrUbnozPCCqcoO= NUTUUIRTSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCxdIXiZYlvKDJiZX7kc4dmdm:3czDn[Y5meyCjY4TpeoF1cW:wIHHmeIVzKDJ2IHjyd{BjgSCzUFPSMEBGSzVyPUCuNFAxO87:TR?= NUD1[|kxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVkzOTNpPkK1OVU6OjF|PD;hQi=>
HEC1 MVnGeY5kfGmxbjDhd5NigQ>? NGH3O20zPCCqcoO= NYTuPHA1SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGg[ZhxemW|c3XkJIlvKGi3bXHuJGhGSzFiY3XscJMh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYX\0[ZIhOjRiaILzJIJ6KEWURT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmVidILhcpNn\WO2aX;uJIF{e2G7LDDFR|UxRTBwMECwN|jPxE1? NWrBUVdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVkzOTNpPkK1OVU6OjF|PD;hQi=>
MCF7 M4jiXWZ2dmO2aX;uJIF{e2G7 M1vSU|ExKG6P NV3IPJBPOjBiaILz NVmzfFJmUW6mdXP0bY9vKG:oIHXzeJJw\2WwIILlZ4VxfG:{LXHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhOTBibl2gZYZ1\XJiMkCgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MmnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{m0PFUoRjJ3OEe5OFg2RC:jPh?=
Saos2 MYHGeY5kfGmxbjDhd5NigQ>? M{\PflEhfU1? NHfC[5U4KGSjeYO= MXvB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGgbY4hcHWvYX6gV4FwezJiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCBzIIXNJIFnfGW{IEeg[IF6eyCkeTDNWHMh[XO|YYm= NHv4cm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G1OVQ4OCd-MkexOVU1PzB:L3G+
Saos2 NVnjWIloTnWwY4Tpc44h[XO|YYm= NGf0eJMxNjFibl2= NEXwT5k{KGSjeYO= NVLRPYQ6SWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGmwIHj1cYFvKFOjb4OyJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEGOUDDhZ5Rqfmm2eTD1d4lv\yCyLX7peJJweGinbonsJJBpd3OyaHH0[UBieyC|dXLzeJJifGViYYSgNE4yKG6PIHHmeIVzKDNiZHH5d{BjgSClb3zvdolu\XS{aXOgZZN{[Xl? MnnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW0O|AoRjJ5MUW1OFcxRC:jPh?=
insect cells MWnGeY5kfGmxbjDhd5NigQ>? NIXxSGlFcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIHj1cYFvKHKnY3;tZolv[W62IH\1cIwhdGWwZ4ToJGVT[WyyaHGg[ZhxemW|c3XkJIlvKGmwc3XjeEBk\WyuczDt[YF{fXKnZDD1dEB1dyB2IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG|c3H5MEBKSzVyPUCuNFA1PM7:TR?= M2nxdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N|c2Lz5{N{[0O|M4PTxxYU6=
insect cells M{LFWmZ2dmO2aX;uJIF{e2G7 MXiyJIhzew>? M4jKT2Rqe3CuYXPlcYVvfCCxZjDmcJVwem2xbnWtcIFj\WynZDDFV|Ih\nKxbTDy[YNwdWKrbnHueEBnfWyuIHzlcod1cCCqdX3hckBGWmGucHjhJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{KGGodHXyJFIhcHK|IHL5JHRTNU[URWSgZZN{[XluIFnDOVA:OC5yMEC0Ne69VQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDFyNUK4N{c,OjhzMEWyPFM9N2F-
insect cells MVnGeY5kfGmxbjDhd5NigQ>? NGfiO5hKdmirYnn0bY9vKG:oIH\seY9zdW:wZTDFV|Ih[mmwZHnu[{B1dyC{ZXPvcYJqdmGwdDDmeYxtKGynbnf0bEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyCkeTDmcJVwemW|Y3XuZ4UheG:uYYLpfoF1cW:wIHHzd4F6NCCLQ{WwQVAvODB4NkZOwG0> MkXRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5M{WyNVQoRjJ6N{O1NlE1RC:jPh?=
Ishikawa cells MnrOSpVv[3Srb36gZZN{[Xl? M2XCdVk3KGi{cx?= MmfBSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZUBqdiCqdX3hckBKe2ira3H3ZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImgeZNqdmdicD3ObZRzd3CqZX7vcEBxcG:|cHjheIUh[XNic4Xid5Rz[XSnIIDy[ZRz\WG2ZXSg[o9zKDl4IHjyd{Bnd2yub4fl[EBjgSC|dXLzeJJifGViYXTkbZRqd25ibXXhd5Vz\WRiZY\ldpkhOTVic3Xjd{BjgSC|Y3HucolvNCCHQ{WwQVAvODByMt88US=> NXzC[mQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4NVI5QTJpPkK4PFEzQDl{PD;hQi=>
293 cells M2DJOmZ2dmO2aX;uJIF{e2G7 M33BPWJqdmSrbnegZYZncW6rdImgeI8hTVJiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gWWFUNWKuYTDHdolxXGm2ZTCyPVMh[2WubIOgZpkhW2WuZXP0JJNkemWnbjDjc41x\XSrdHn2[UBjcW6maX7nJIF{e2G7LDDFR|UxRTBwMECyN:69VQ>? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh6Nkm5Okc,Ojh6OE[5PVY9N2F-
HEk293 NEPLVWZHfW6ldHnvckBie3OjeR?= MoPaRYdwdmm|dDDhZ5Rqfmm2eTDheEBz\WOxbXLpcoFvfCCqdX3hckBISUx2LX\1d4VlKEWUYXzwbIEh\XiycnXzd4VlKGmwIFjFb|I6OyClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFAyOc7:TR?= NWfkTHlWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlU{OzVpPkK5OVI2OzN3PD;hQi=>
Ishikawa MVXGeY5kfGmxbjDhd5NigQ>? MmTmPVYhcHK| NHvke2hGe3S{b3flcolkKGGldHn2bZR6KGG2IFXSZYxxcGFiaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGGldHn2bZR6KHW|aX7nJJAudmm2cn;wbIVvd2xiYYOgd5Vje3S{YYTlJJRz\WG2ZXSg[o9zKDl4IHjyd{Bnd2yub4fl[EBjgSC|dXLzeJJifGViYXTkbZRqd25iYomgd5Bm[3S{b4Doc5RwdWW2cnnjJI1mfGixZDygSWM2OD1yLkCwNFA{|ryP M1PuXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkSxNlA3Lz5{OU[0NVIxPjxxYU6=
MDA-MB-231/beta41 NH\C[m5HfW6ldHnvckBie3OjeR?= MoHUNVghcHK| MYnFd5Rzd2enbnnjJIFkfGm4aYT5JIF1KEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCqdX3hckBOTEFvTVKtNlMyN2KndHG0NUBk\WyuczDh[pRmeiBzODDodpMh[nlicnXubYxt[SCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFAxO87:TR?= NGjYVI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0NVIxPid-Mkm2OFEzODZ:L3G+
insect cells M4H3RWZ2dmO2aX;uJIF{e2G7 MYSyJIhzew>? MnH1TY5pcWKrdHnvckBw\iCobIXvdo9ud26nIHLpcoRqdmdidH:gdoVkd22kaX7hcpQhcHWvYX6g[pVtdCCuZX7neIghfW62YXfn[YQhTVKkZYThJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgZYZ1\XJiMjDodpMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCjc4PhfUwhUUN3ME2wMlAxOTJ6zszN MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTh4OUWwN{c,Ojl6Nkm1NFM9N2F-
insect cells MoXQSpVv[3Srb36gZZN{[Xl? MWWyJIhzew>? MnS4TY5pcWKrdHnvckBw\iCobIXvdo9ud26nIHLpcoRqdmdidH:gdoVkd22kaX7hcpQhcHWvYX6g[pVtdCCuZX7neIghfW62YXfn[YQhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJIFnfGW{IEKgbJJ{KGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iYYPzZZktKEmFNUC9NE4xODR6MEVOwG0> NVi4dJZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4Olk2ODNpPkK5PFY6PTB|PD;hQi=>
insect cells M1j6NWZ2dmO2aX;uJIF{e2G7 NGDvb3YxNjFidH:gNVAxODBidV2= M1niT2JqdmSrbnegZYZncW6rdImgeI8hfW62YXfn[YQhcHWvYX6gdoVkd22kaX7hcpQh\nWubDDs[Y5ofGhiRWLhcJBp[SCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{KDBwMTD0c{AyODByMDD1UUBjgSCIbIXvdo1wdmViRWOyJGdz\WWwIHTpd5Bt[WOnbXXueEBj[XOnZDDhd5NigQ>? MoPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByOUG5NlAoRjNyMEmxPVIxRC:jPh?=
293 cells MnfGSpVv[3Srb36gZZN{[Xl? M4TnbmFod26rc4SgZYN1cX[rdImgZZQhT0GONDDEUmEu[mmwZHnu[{Bld22jaX6g[pV{\WRiRWLhcJBp[SBqdX7rco94diCxcnnnbY4qKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDlfJBz\XO|ZXSgbY4hXUGVLXLsZUBIemmyVHn0[UAzQTNiY3XscJMh[XO|ZYPz[YQh[XNiYnX0ZU1t[WO2YX3hd4UhfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[nliRmLFWE1j[XOnZDDH[Y5mSkyDenXyJIF{e2G7LDDJR|UxRTBwMECwNVA4|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF2NE[5O{c,OzBzNES2PVc9N2F-
293 cells M3LmcWZ2dmO2aX;uJIF{e2G7 MW\B[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRiRF7BMYJqdmSrbneg[I9u[WmwIH\1d4VlKEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gWWFUNWKuYTDHdolxXGm2ZTCyPVMh[2WubIOgZZN{\XO|ZXSgZZMh[mW2YT3sZYN1[W2jc3WgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZpkhTlKHVD3iZZNm\CCJZX7lRmxCgmW{IHHzd4F6NCCLQ{WwQVAvODByNUe5{txO MoiyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzNES2PVcoRjNyMUS0Olk4RC:jPh?=
HEK293 M{f1U2Z2dmO2aX;uJIF{e2G7 MYHT[Yxm[3SrdnWg[ZN1em:pZX6gdoVk\XC2b4Kg[I94di2{ZXf1cIF1d3JiYXP0bZZqfHliYYSgSmxCTy22YXfn[YQhTVKjbIDoZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIFXSZYxxcGFiZHXndoFl[XSrb36gZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMECwNFnPxE1? NWjWS2NYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C5OFA2PjVpPkOwPVQxPTZ3PD;hQi=>
HEK293 MknhSpVv[3Srb36gZZN{[Xl? M3fuS2Fod26rc4SgZYN1cX[rdImgZZQhTkyDRz30ZYdo\WRiRWLhcJBp[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJGVTNWGucHjhMY1m\GmjdHXkJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECxN:69VQ>? NIHmNIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEm0NFU3PSd-M{C5OFA2PjV:L3G+
Sf9 M1;JO2Z2dmO2aX;uJIF{e2G7 MlHnRolv\GmwZzDBd5NigTpiVHjlJI1mfGixZDDlcZBtd3mnZDD3ZZMh[WSjcITl[EBnem:vIITo[UB{[2mnboTp[olkKGyrdHXyZZR2emViYX7kJIRme2O{aXLl[EBqdiCmZYThbYwh[nliT4Pic5VzdiCndDDhcE4hMDF7OUOsJGJqd2OqZX3pd5RzgSxiM{KsJFYzOjlvNkKzOkkvKFKnY3;tZolv[W62IHj1cYFvKEWUYXzwbIEh[W6mIFXSVkBxem:2ZXnud{B4\XKnIID1dolncWWmIH\yc40hfHKjboPm[YN1\WRiU3[5MYNmdGy|LjDUbIUhcW5idnn0do8hNCCNaU2wMlAxODBzNd88US=> NVfFNXZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN3Lze+R4hGVUKOPD;hQi=>
Sf9 NHrNWXZHfW6ldHnvckBie3OjeR?= NYXYe2pGUW6qaXLpeIlwdiCDc4PhfVohXGinIH3leIhw\CCnbYDsc5lm\CC5YYOgZYRieHSnZDDmdo9uKHSqZTDzZ4lmdnSrZnnjJIxqfGW{YYT1doUh[W6mIHTld4NzcWKnZDDpckBl\XSjaXygZpkhV3Okb4XtJIV1KGGuLjCoNVk6OyxiQnnvZ4hmdWm|dIL5MEA{OixiNkKyPU03OjN4KT6gVoVkd22kaX7hcpQhcHWvYX6gSXJidHCqYTDhcoQhTVKkZYThJJBzd3SnaX7zJJdmemVicIXybYZq\WRiZoLvcUB1emGwc3\lZ5Rm\CCVZkmtZ4VtdHNwIGTo[UBqdiC4LDDLbV0xNjByMECxOe69VQ>? Ml:zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
HEK293T MXfGeY5kfGmxbjDhd5NigQ>? M1nYXVI1KGi{cx?= NWfLcHhwSWexbnnzeEBi[3Srdnn0fUBifCCHUj3i[ZRiKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gTGVMOjl|VDDj[YxteyCjZoTldkAzPCCqcoOgZpkhW3SnYXT5MWdtdyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFAxOzl6zszN MkXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
HEK293T MYLGeY5kfGmxbjDhd5NigQ>? NIfwXHYzPCCqcoO= NGTWdppC\2:waYP0JIFkfGm4aYT5JIF1KEWULXHsdIhiKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gTGVMOjl|VDDj[YxteyCjZoTldkAzPCCqcoOgZpkhW3SnYXT5MWdtdyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFAy|ryP MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|UwLz6FaFXNRmw9N2F-
Sf9 MWjGeY5kfGmxbjDhd5NigQ>? Mmf1Rolv\GmwZzDBd5NigTpiVHjlJI1mfGixZDDlcZBtd3mnZDD3ZZMh[WSjcITl[EBnem:vIITo[UB{[2mnboTp[olkKGyrdHXyZZR2emViYX7kJIRme2O{aXLl[EBqdiCmZYThbYwh[nliT4Pic5VzdiCndDDhcE4hMDF7OUOsJGJqd2OqZX3pd5RzgSxiM{KsJFYzOjlvNkKzOkkvKFKnY3;tZolv[W62IHj1cYFvKEWUYXzwbIEh[W6mIFXSVkBxem:2ZXnud{B4\XKnIID1dolncWWmIH\yc40hfHKjboPm[YN1\WRiU3[5MYNmdGy|LjDUbIUhcW5idnn0do8hNCCNaU2wMlAxODJzzszN M3LrOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{PS9pPlPoSW1DVDxxYU6=
Sf9 NVPzXWJLTnWwY4Tpc44h[XO|YYm= NFmy[mtKdmirYnn0bY9vKEG|c3H5PkBVcGVibXX0bI9lKGWvcHzvfYVlKHejczDh[IFxfGWmIH\yc40hfGinIIPjbYVvfGmoaXOgcIl1\XKjdIXy[UBidmRiZHXzZ5Jq[mWmIHnuJIRmfGGrbDDifUBQe2KxdX2g[ZQh[WxwIDixPVk{NCCEaX;jbIVucXO2comsJFMzNCB4MkK5MVYzOzZrLjDS[YNwdWKrbnHueEBpfW2jbjDFVoFteGijIHHu[EBGWmKndHGgdJJwfGWrboOge4Vz\SCydYLp[olm\CCocn;tJJRz[W6|ZnXjeIVlKFOoOT3j[Yxtey5iVHjlJIlvKHZuIFvpQVAvODByMkJOwG0> NE\Wfoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
CV-1 MknIUJVkcW[ncnHz[UBCe3OjeR?= M1q4Xmx2[2moZYLhd4UhSXO|YYm6JGV{fHKxZ3XuJJJm[2WydH;yMY5m\2G2aY\lJGNXNTFia3nkcoV6KGOnbHzzJIFz\SCvYXnueIFqdmWmIHnuJGR2dGKnY3PvK5MhdW:maX\p[YQhTWGpbHWnd{Bu\WSrdX2ge4l1cCB2LkWg[{9NKGeudXPvd4Uhe3WycHzlcYVvfGWmIIfpeIghOTBnIH\leIFtKGKxdnnu[UB{\XK3bTDhcoQhOTByIIXubZR{N22uIIDlcolkcWyuaX6td5Rz\XC2b335Z4lvKGG2IEO3xtAhSy5iaX6gZUBpfW2rZHnmbYVlKDVnIFPPNkBifG1uIFnDOVA:OC5yMEG3{txO NUfsTmYzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN3Lze+R4hGVUKOPD;hQi=>
CV-1 MmPHUJVkcW[ncnHz[UBCe3OjeR?= NU\XSHhmVHWlaX\ldoF{\SCDc4PhfVohTXO2cn;n[Y4hemWlZYD0c5IudmWpYYTpeoUhS1ZvMTDrbYRv\XliY3XscJMh[XKnIH3hbY51[WmwZXSgbY4hTHWuYnXjZ48oeyCvb3Tp[olm\CCHYXfs[Ud{KG2nZHn1cUB4cXSqIESuOUBoN0xiZ3z1Z49{\SC|dYDwcIVu\W62ZXSge4l1cCBzMDWg[oV1[WxiYn;2bY5mKHOncoXtJIFv\CBzMECgeY5qfHNxbXygdIVvcWOrbHzpck1{fHKncITvcZlkcW5iYYSgN|fDuCCFLjDpckBiKGi3bXnkbYZq\WRiNTWgR28zKGG2bTygTWM2OD1yLkCwNVnPxE1? MmLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
COS7 NF7re4lHfW6ldHnvckBie3OjeR?= NWHIOVFUS2:vcHX0bZRqfmViYnnu[Ilv\yCjZn\pcol1gSC2bzDHMZBzd3SnaX6gZ492eGynZDDld5Rzd2enbjDy[YNmeHSxcjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGK7IFWyJGFt\XijIE[zN{B{fGGrbnnu[{Bj[XOnZDDmcJVwemW|Y3XuZ4Uh[W6jbInzbZMtKEurPUCuNFAzP87:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|UwLz6FaFXNRmw9N2F-
MDA-MB-231 NH3kOGxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoqwNUBxVSC2bzCxJO69VQ>? M1L2eVQ5KGi{ MoTxS5Jwf3SqIHnubIljcXSrb36gc4YhUG:vbzDzZZBq\W6|IDjoeY1idiliTVTBMW1DNTJ|MTDj[YxteyCjdDCxNEcuOTJidH:gNVAoNTZiTTDh[pRmeiB2ODDodkBjgSCPVGSgZZN{[Xl? NH;vSGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
UMR106 NFjGRpJHfW6ldHnvckBie3OjeR?= MlyyNVAhdk1? M1vScVI1KGi{cx?= MX7UdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iRWKtZYxxcGFidILhcpNn\WO2ZXSgbY4hemG2IGXNVlExPiClZXzsd{BifCBzMDetPEBOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NEOzcoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
UMR106 MlXaSpVv[3Srb36gZZN{[Xl? M{L3VlExKG6P NFn5PHQzPCCqcoO= NFK5PIVVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gSXIu[mW2YTD0doFve2[nY4Tl[EBqdiC{YYSgWW1TOTB4IHPlcIx{KGG2IEGwK{05KE1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 M1nEWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{PS9pPlPoSW1DVDxxYU6=
U2OS M2jtUmZ2dmO2aX;uJIF{e2G7 MneyNE4xOSC3TR?= M3j0TFI1KGi{cx?= M2Ll[WFkfGm4YYTpc44hd2ZiRWLi[ZRiKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKFV{T2OgZ4VtdHNiYYSgNE4xOSC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN|UwLz6FaFXNRmw9N2F-
U2OS Ml:wSpVv[3Srb36gZZN{[Xl? NGnSVpExNjBzIIXN Mmq3NlQhcHK| NF3oN|JC[3SrdnH0bY9vKG:oIFXSZYxxcGFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iVULPV{Bk\WyuczDheEAxNjBzIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NHXDRno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
Assay
Methods Test Index PMID
Western blot p-GSK3β / GSK3 ; β-catenin ; Survivin / ER-alpha / NgBR ; ERα / FOXP1 / KRAS / PTEN 19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05175105 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05144256 Not yet recruiting Drug: Mitapivat|Drug: Mitapivat-matching placebo Pediatric Pyruvate Kinase Deficiency|Pediatric Hemolytic Anemia Agios Pharmaceuticals Inc. March 1 2022 Phase 3
NCT05096169 Not yet recruiting Drug: Clomiphene Citrate 25mg|Drug: Clomiphene Citrate 50mg Testosterone Deficiency Weill Medical College of Cornell University December 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Estradiol | Estradiol ic50 | Estradiol price | Estradiol cost | Estradiol solubility dmso | Estradiol purchase | Estradiol manufacturer | Estradiol research buy | Estradiol order | Estradiol mouse | Estradiol chemical structure | Estradiol mw | Estradiol molecular weight | Estradiol datasheet | Estradiol supplier | Estradiol in vitro | Estradiol cell line | Estradiol concentration | Estradiol nmr