Estradiol

Catalog No.S1709

Estradiol Chemical Structure

Molecular Weight(MW): 272.38

Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone.

Size Price Stock Quantity  
In DMSO USD 270 In stock
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 cells NUDnXJRPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWLsWpE6T3Kxd4ToJJJme3CxboPlJIlvKGO3bIT1doV{KG:oIF3DSk04KCiqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZTmgZ4VtdHNuIFXDOVA:OS53MnWtNFYh|ryP M{f4fVk{PTd3M{O=
human MCF7 cells M{DWO2Z2dmO2aX;uJIF{e2G7 NFTqS2w3KGSjeYO= NEDNRpVGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IE[g[IF6eyCkeTDXV3Q5KGG|c3H5MEBGSzVyPUOuO4UuODZizszN NInVeoUyQDd4ME[wNy=>
human U2-OS cells M2fWZWZ2dmO2aX;uJIF{e2G7 M2fheWFkfGm4YYTpc44hd2ZiaIXtZY4hTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaIXtZY4hXTJvT2OgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:uLDDFR|UxRTSnLUC2JO69VQ>? NE\3T|QzOzB2M{K0Ni=>
MCF7 cells NFzBcXJRem:uaX\ldoF1cW:wIHHzd4F6 MV23NkBp M1zD[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6t[IVncWOrZX70JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD14LkTlMVA3KM7:TR?= M4LwRlIzPDZ2Nkix
HeLa cells NUTaZpdZTnWwY4Tpc44h[XO|YYm= NVX2ZnJLSWO2aY\heIlwdiCxZjDld5Rzd2enbjDy[ZNxd26|ZTDlcIVu\W62IHnuJGhmVGFiY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iRYP0do9o\W5icnXj[ZB1d3JiYXzwbIEtKEWFNUC9NoUuODVizszN NWj4XFRlOTF7ME[yPFA>
HEK293 cells NVr0dGtNTnWwY4Tpc44h[XO|YYm= NUi3dJFWSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTJwNHWtNFUh|ryP NFfYSoIzPTNyNU[4PC=>
human Ishikawa cells MVjGeY5kfGmxbjDhd5NigQ>? M{H6c|czKGh? NEfx[5NGe3S{b3flcolkKGGldHn2bZR6KGG2IHXzeJJw\2WwIILlZ4VxfG:{IHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBidGujbHnu[UBxcG:|cHjheIF{\SCneIDy[ZN{cW:wIHHmeIVzKDd{IHjyd{BjgSCvaXPyc5Bt[XSnIILlZYRmeixiRVO1NF0zNjR4ZT2wOUDPxE1? NYrQVoRVOjJ2NkS2PFE>
CHO-K1 cells NVezdI15TnWwY4Tpc44h[XO|YYm= NH71T3gzPCCq NHzrSIFVemGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCHUnLleIEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVMvPmVvMEWg{txO NUfafGtiOjJ4NEeyNVc>
T47D cells MVzGeY5kfGmxbjDhd5NigQ>? NVz3Oop{SWexbnnzeEBm\m[nY4Sgc44hfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hcW5iVES3SEBk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijLDDFR|UxRTenLUC1JO69VQ>? NIXUWHUyOjR3OUCxOy=>
human HEC1 cells NWfXZ5p5TnWwY4Tpc44h[XO|YYm= NUSxUHpqOjRiaB?= NVXqeHNtSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBnfWyuLXzlcod1cCCHUnHsdIhiKHKnY3XweI9zKGW6cILld5Nm\CCrbjDoeY1idiCKRVOxJINmdGy|IHPvMYV5eHKnc4PpcochMEWURTmyMZBUOi2udXOg[4Vv\SCjc4Pld5Nm\CCjczD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUnlMVA2KM7:TR?= NEPCNnYzOTR6MUS5Oy=>
human SK-BR-3 cells MWTGeY5kfGmxbjDhd5NigQ>? M{fZNVQ5KGh? MkHVTY5pcWKrdHnvckBw\iClaHXtc4F1fHKjY4ThcpQucW6mdXPl[EBk\WyuIH3p[5JifGmxbjDv[kBpfW2jbjDTT{1DWi1|IHPlcIx{KGGodHXyJFQ5KGi{czDifUB1emGwc4flcIwhdWmpcnH0bY9vKGG|c3H5MEBKSzVyPUCuNUBvVQ>? Mn\JNVY2OjB5M{O=
UAS cells M3HOVmZ2dmO2aX;uJIF{e2G7 NY\HOFZxOThiaB?= NWDabWNQSWexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEh\XiycnXzd4VlKGmwIGXBV{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGKndHGtcIFkfGGvYYPlJIFnfGW{IEG4JIhzeyCkeTDi[ZRiNWyjY4ThcYF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMkWgcm0> NIHYco0zOTh6NUK3PS=>
COS7 cells M1HSNWZ2dmO2aX;uJIF{e2G7 NWLrV5RJTGm|cHzhZ4Vu\W62IH;mJGUzNUGuZYjhOlM{KG[{b32gS2ZRNXSjZ3fl[EBGWmGucHjhJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHL5JGZCS1NuIFvpQVAvOyCwTR?= MV:xOlUzODd|Mx?=
AH109 cells Mm\qSpVv[3Srb36gZZN{[Xl? NYrRbld5OjRiaB?= MojCRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCHQ{WwQVEvODVibl2= NH3oXWczOjZ2N{KxOy=>
HepG2 cells MWPGeY5kfGmxbjDhd5NigQ>? NF7ZfIYzPCCq NVvhVHB7SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCKZYDHNkBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUKuNkBvVQ>? MVSyNlI5OzN{OB?=
COS-1 cells M4TuVmZ2dmO2aX;uJIF{e2G7 MljYTY4hfmm2cn:gZYdwdmm|dDDhZ5Rqfmm2eTDmc5Ih\XO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGg[ZhxemW|c3XkJIlvKEORUz2xJINmdGy|LDDFR|UxRTJwNDDuUS=> M{ixOlE2PzF|NEC3
SAOS-2 cells NEPpZnVHfW6ldHnvckBie3OjeR?= NFTCXm1HfW6ldHnvcoFtKEW|dILv[4VvKHKnY3XweI9zKGKndHGgZYN1cX[rdImgZpkhdWW2YXzsc5RpcW:wZXnuJIlv\HWldHnvckBqdiCVQV;TMVIh[2WubIOsJGVFPTB;ND63JI5O NFu5cIoyOjh{NEC0Ny=>
MDA-MB-231 cells Mn7OVJJwdGmoZYLheIlwdiCjc4PhfS=> MmXONkBl[Xm| NE\SSHlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbjDFVk1v\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;M{OuOFYh|ryP MWiyOVIzOjh5Nh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.25 mM)
Ethanol 5 mg/mL (18.35 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03718338 Not yet recruiting Procedure: Evaluation|Other: Quality-of-Life Assessment Prostate Adenocarcinoma University of Washington|National Cancer Institute (NCI) November 30 2019 Not Applicable
NCT04093856 Not yet recruiting -- Diabetes Mellitus|Diabetes Mellitus Type 2|Obesity|Body Weight|Bone Diseases Metabolic|Fractures Bone|Osteoporotic Fractures|Osteoporosis|Quality of Life|Fall|Musculoskeletal Diseases Suzanne Morin|CHU de Quebec-Universite Laval|McGill University Health Centre/Research Institute of the McGill University Health Centre October 2019 --
NCT04007614 Not yet recruiting Other: Questionnaire Contraception|Macro-progestins Assistance Publique - Hôpitaux de Paris September 2019 --
NCT03680053 Not yet recruiting Drug: Duphaston|Drug: Cetrorelix Infertility|ART ShangHai Ji Ai Genetics & IVF Institute September 2019 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Estrogen/progestogen Receptor Signaling Pathway Map

Related Estrogen/progestogen Receptor Products

Tags: buy Estradiol | Estradiol ic50 | Estradiol price | Estradiol cost | Estradiol solubility dmso | Estradiol purchase | Estradiol manufacturer | Estradiol research buy | Estradiol order | Estradiol mouse | Estradiol chemical structure | Estradiol mw | Estradiol molecular weight | Estradiol datasheet | Estradiol supplier | Estradiol in vitro | Estradiol cell line | Estradiol concentration | Estradiol nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID