Estradiol

For research use only.

Catalog No.S1709 Synonyms: 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol

10 publications

Estradiol Chemical Structure

CAS No. 50-28-2

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

Selleck's Estradiol has been cited by 10 publications

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HepG2 NHXMUnhHfW6ldHnvckBie3OjeR?= NXLUUVRtOjRiaILz Ml36RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUJOwG0> M3G4WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN|I5Lz5{MkK4N|MzQDxxYU6=
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Ishikawa cells M2\ZbGZ2dmO2aX;uJIF{e2G7 MVK3NkBpenN? NXTxUoVzTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDld5Rzd2enbjDy[YNmeHSxcjDpckBpfW2jbjDJd4hqc2G5YTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WytYXzpcoUheGixc4DoZZRie2ViZYjwdoV{e2mxbjDh[pRmeiB5MjDodpMh[nlibXnjdo9xdGG2ZTDy[YFl\XJuIFXDOVA:OC5yMECwNlQ3|ryP NYjhbldDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
Ishikawa cells NX\sfFFQTnWwY4Tpc44h[XO|YYm= MlTpNE4yKG6P NE\E[ZA4OiCqcoO= NFPkdmJG\m[nY4Sgc44h[2WubDDwdo9tcW[ncnH0bY9vKG:oIHj1cYFvKEm|aHnrZZdiKGOnbHzzJIF1KDBwMTDuUUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5 NYPHZVFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
CHO-K1 MV\GeY5kfGmxbjDhd5NigQ>? Mn\6NlQhcHK| NHrP[opVemGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCHUnLleIEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMEO2{txO MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
CHO-K1 MYXGeY5kfGmxbjDhd5NigQ>? MUKyOEBpenN? NYrS[FFLXHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hd2ZiRWLhcJBp[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOe69VQ>? M3TrR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O|IyPzxxYU6=
yeast AH109 MknaSpVv[3Srb36gZZN{[Xl? NU[5WW9oOjRiaILz NUfxd4xbSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKHKnY3XweI9zKEyERDDlfJBz\XO|ZXSgbY4hgWWjc4SgRWgyODliY3XscJMh[XO|ZYPz[YQh[XNiY3;tdI92dmRiY3;uZ4VvfHKjdHnvckBifCC5aHnjbEAyOCViYXfvcol{fCCjY4Tpeol1gSCxZjD0bIUhdWG6aX31cUBi[3Srdnn0fUBw\iCHMjDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiQXP0bZZqfHl;MD6wNFAy|ryP NULETlA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
yeast AH109 MV;GeY5kfGmxbjDhd5NigQ>? NWTLUIVvOjRiaILz NEnpfm5C\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W|c3XkJIF{KGOxbYDveY5lKGOxbnPlcpRz[XSrb36gZZQhf2irY3igNVAmKGGpb37pd5Qh[WO2aY\peJkhd2ZidHjlJI1igGmvdX2gZYN1cX[rdImgc4YhTTJiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEGldHn2bZR6RTBwMECwNVjPxE1? MlPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109 NGXpV5FHfW6ldHnvckBie3OjeR?= MkLMNlQhcHK| MXnB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGgdoVk\XC2b4KgUGJFKGW6cILld5Nm\CCrbjD5[YF{fCCDSEGwPUBk\WyuczDhd5Nme3OnZDDhd{BqdnSncnHjeIlwdiC5aYToJHNTSzFiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEWFNUC9NE4xODFyNd88US=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109 NEjnVlRHfW6ldHnvckBie3OjeR?= MXSyOEBpenN? MVXB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDy[YNmeHSxcjDMRmQh\XiycnXzd4VlKGmwIInlZZN1KEGKMUC5JINmdGy|IHHzd4V{e2WmIHHzJIlvfGW{YXP0bY9vKHerdHigV3JEOSCjZoTldkAzPCCqcoOgZpkh[WyyaHGt[4Ft[WO2b4Pp[IF{\SCjc4PhfUwhTUN3ME2wMlAxOTB6zszN NGrMTog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O|IyPyd-MkK2OFczOTd:L3G+
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U2-OS MlHrSpVv[3Srb36gZZN{[Xl? M{PQfGFkfGm4YYTpc44hd2ZiaIXtZY4hTVKkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBWOi2RUzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZkhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2xuIFXDOVA:OC5yMECwNe69VQ>? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.25 mM)
Water Insoluble
Ethanol '5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage powder
in solvent
Synonyms 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol
Smiles CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04189133 Not yet recruiting Drug: Lutropin alfa|Drug: Human chorionic gonadotropin Acquired Hypogonadotropic Hypogonadism Azienda Ospedaliero-Universitaria di Modena October 1 2021 Phase 2
NCT05002738 Not yet recruiting Drug: Desogestrel and Ethinyl Estradiol Tablets Contraception University of Colorado Denver September 2021 Phase 4
NCT05021094 Not yet recruiting Drug: Kuntai Capsule|Drug: Femoston Primary Ovarian Insufficiency Affiliated Hospital of Nantong University September 1 2021 Phase 4
NCT05003336 Not yet recruiting Drug: Xiangshao Granules|Drug: Xiangshao Granules Placebo Anxiety|Menopause Peking Union Medical College Hospital September 2021 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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