Shipping services continue despite COVID-19 outbreak ! Click here to view COVID-19 related products

Estradiol

For research use only.

Catalog No.S1709 Synonyms: 17β-estradiol

8 publications

Estradiol Chemical Structure

CAS No. 50-28-2

Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 270 In stock
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Estradiol has been cited by 8 publications

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 cells MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUHZNnhnT3Kxd4ToJJJme3CxboPlJIlvKGO3bIT1doV{KG:oIF3DSk04KCiqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZTmgZ4VtdHNuIFXDOVA:OS53MnWtNFYh|ryP MUO5N|U4PTN|
human MCF7 cells Mmm4SpVv[3Srb36gZZN{[Xl? NYL1flRtPiCmYYnz NYjVcpQ3TXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJINmdGxicILvcIln\XKjdHnvckBi\nSncjC2JIRigXNiYomgW3NVQCCjc4PhfUwhTUN3ME2zMldmNTB4IN88US=> MkXWNVg4PjB4MEO=
human U2-OS cells NIr5d4JHfW6ldHnvckBie3OjeR?= Mme4RYN1cX[jdHnvckBw\iCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCqdX3hckBWOi2RUzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZkhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2xuIFXDOVA:PGVvME[g{txO MWqyN|A1OzJ2Mh?=
MCF7 cells NITXNnFRem:uaX\ldoF1cW:wIHHzd4F6 NHXp[o44OiCq MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwLXTl[olkcWWwdDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Pi52ZT2wOkDPxE1? NXvFVlA6OjJ2NkS2PFE>
HeLa cells NGnUcZRHfW6ldHnvckBie3OjeR?= MXzBZ5RqfmG2aX;uJI9nKGW|dILv[4VvKHKnc4DvcpNmKGWuZX3lcpQhcW5iSHXMZUBk\WyuczDzeIFjdHlidILhcpNn\WO2ZXSge4l1cCCqdX3hckBGe3S{b3flckBz\WOncITvdkBidHCqYTygSWM2OD1{ZT2wOUDPxE1? NEPDWZAyOTlyNkK4NC=>
HEK293 cells MmfWSpVv[3Srb36gZZN{[Xl? M1Lod2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0zNjSnLUC1JO69VQ>? MW[yOVMxPTZ6OB?=
human Ishikawa cells NIG4[HNHfW6ldHnvckBie3OjeR?= NGnPW244OiCq MoDkSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxs[WyrbnWgdIhwe3CqYYThd4Uh\XiycnXzd4lwdiCjZoTldkA4OiCqcoOgZpkhdWmlcn;wcIF1\SC{ZXHk[ZItKEWFNUC9Nk41PmVvMEWg{txO MYGyNlQ3PDZ6MR?=
CHO-K1 cells M{\zfWZ2dmO2aX;uJIF{e2G7 MnXZNlQhcA>? Mk[5WJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gc4YhTVKkZYThJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tT|Eh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2zMlZmNTB3IN88US=> M2TvclIzPjR5MkG3
T47D cells NWjZTplFTnWwY4Tpc44h[XO|YYm= NFvBdm9C\2:waYP0JIVn\mWldDDvckB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBqdiCWNEfEJINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZYxxcGFuIFXDOVA:P2VvMEWg{txO NH7IcHEyOjR3OUCxOy=>
human HEC1 cells MUnGeY5kfGmxbjDhd5NigQ>? NIfRXYUzPCCq M4\MUmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6g[pVtdC2uZX7neIghTVKjbIDoZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iaIXtZY4hUEWFMTDj[YxteyClbz3lfJBz\XO|aX7nJEhGWkVrMj3wV|IudHWlIHflcoUh[XO|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD17ZT2wOUDPxE1? M2rPfFIyPDhzNEm3
human SK-BR-3 cells MUjGeY5kfGmxbjDhd5NigQ>? NES5OZk1QCCq MVnJcohq[mm2aX;uJI9nKGOqZX3vZZR1emGldHHueE1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIGPLMWJTNTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJRz[W6|d3XscEBucWe{YYTpc44h[XO|YYmsJGlEPTB;MD6xJI5O NIHrWHAyPjV{MEezNy=>
UAS cells MXjGeY5kfGmxbjDhd5NigQ>? NUj3OJpCOThiaB?= M{TTXGFod26rc4SgZYN1cX[rdImgZZQhTVKkZYThJIV5eHKnc4Pl[EBqdiCXQWOgZ4VtdHNiY3:t[ZhxemW|c3nu[{Bj\XSjLXzhZ5RidWG|ZTDh[pRmeiBzODDodpMh[nliYnX0ZU1t[WO2YX3hd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjJ3IH7N M1XhdFIyQDh3Mke5
COS7 cells NFu0Sm9HfW6ldHnvckBie3OjeR?= M{LJXGRqe3CuYXPlcYVvfCCxZjDFNk1CdGW6YU[zN{Bnem:vIFfGVE11[WepZXSgSXJidHCqYTDlfJBz\XO|ZXSgbY4hS0:VNzDj[YxteyCkeTDGRWNUNCCNaU2wMlMhdk1? MYWxOlUzODd|Mx?=
AH109 cells NF\PemZHfW6ldHnvckBie3OjeR?= M1;Z[lI1KGh? M{nGZmFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W|c3XkJIF{KGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiRVO1NF0yNjB3IH7N NHH0SmozOjZ2N{KxOy=>
HepG2 cells NGfsSlJHfW6ldHnvckBie3OjeR?= NFTEbXUzPCCq Mn:zRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoFteGijIHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTJwMjDuUS=> MYOyNlI5OzN{OB?=
COS-1 cells M{HQcWZ2dmO2aX;uJIF{e2G7 MVHJckB3cXS{bzDh[49vcXO2IHHjeIl3cXS7IH\vdkBme3S{b3flckBz\WOncITvdkBidHCqYTDlfJBz\XO|ZXSgbY4hS0:VLUGgZ4VtdHNuIFXDOVA:Oi52IH7N NFXLVngyPTdzM{SwOy=>
SAOS-2 cells NXPBO4hpTnWwY4Tpc44h[XO|YYm= MV3GeY5kfGmxbnHsJGV{fHKxZ3XuJJJm[2WydH;yJIJmfGFiYXP0bZZqfHliYomgcYV1[Wyub4TobY9v\WmwIHnu[JVkfGmxbjDpckBUSU:VLUKgZ4VtdHNuIFXEOVA:PC55IH7N NXnVNng1OTJ6MkSwOFM>
MDA-MB-231 cells MVLQdo9tcW[ncnH0bY9vKGG|c3H5 M1fI[FIh\GG7cx?= NHvrRYdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbjDFVk1v\WejdHn2[UBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTB;M{OuOFYh|ryP NUKwVJZSOjV{MkK4O|Y>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.25 mM)
Ethanol 5 mg/mL (18.35 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage powder
in solvent
Synonyms 17β-estradiol
Smiles CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04189406 Not yet recruiting Other: Venapunction Turner Syndrome|Infertility Female|Premature Ovarian Failure|Gonadal Dysgenesis|Premature Menopause|Sex Chromosome Disorders|Ovarian Diseases Radboud University December 1 2020 --
NCT04308486 Not yet recruiting -- Non-obstructive Azoospermia Assiut University December 2020 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Estrogen/progestogen Receptor Signaling Pathway Map

Related Estrogen/progestogen Receptor Products

Tags: buy Estradiol | Estradiol ic50 | Estradiol price | Estradiol cost | Estradiol solubility dmso | Estradiol purchase | Estradiol manufacturer | Estradiol research buy | Estradiol order | Estradiol mouse | Estradiol chemical structure | Estradiol mw | Estradiol molecular weight | Estradiol datasheet | Estradiol supplier | Estradiol in vitro | Estradiol cell line | Estradiol concentration | Estradiol nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID