Estradiol

For research use only.

Catalog No.S1709 Synonyms: 17β-estradiol

5 publications

Estradiol Chemical Structure

CAS No. 50-28-2

Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

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Description Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 cells MkLoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk[5S5Jwf3SqIILld5BwdnOnIHnuJIN2dHS3cnXzJI9nKE2FRj23JEhpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\SliY3XscJMtKEWFNUC9NU42OmVvME[g{txO M3\DTlk{PTd3M{O=
human MCF7 cells NXy3U5J3TnWwY4Tpc44h[XO|YYm= NHzwepU3KGSjeYO= MofPSZN1em:pZX7pZ{Bi[3Srdnn0fUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwheHKxbHnm[ZJifGmxbjDh[pRmeiB4IHThfZMh[nliV2PUPEBie3OjeTygSWM2OD1|LkflMVA3KM7:TR?= M{TlT|E5PzZyNkCz
human U2-OS cells NE\KPW9HfW6ldHnvckBie3OjeR?= M3f0N2FkfGm4YYTpc44hd2ZiaIXtZY4hTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaIXtZY4hXTJvT2OgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:uLDDFR|UxRTSnLUC2JO69VQ>? M{\DOVI{ODR|MkSy
MCF7 cells M1PvcHBzd2yrZnXyZZRqd25iYYPzZZk> NXnOV201PzJiaB?= MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwLXTl[olkcWWwdDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Pi52ZT2wOkDPxE1? NFi4cWozOjR4NE[4NS=>
HeLa cells MnPNSpVv[3Srb36gZZN{[Xl? MnvGRYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGsJGVEPTB;MnWtNFUh|ryP NXrEZ41GOTF7ME[yPFA>
HEK293 cells MXnGeY5kfGmxbjDhd5NigQ>? MVzB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9Nk41\S1yNTFOwG0> MmnsNlU{ODV4OEi=
human Ishikawa cells MWHGeY5kfGmxbjDhd5NigQ>? NEfF[pQ4OiCq MkjKSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxs[WyrbnWgdIhwe3CqYYThd4Uh\XiycnXzd4lwdiCjZoTldkA4OiCqcoOgZpkhdWmlcn;wcIF1\SC{ZXHk[ZItKEWFNUC9Nk41PmVvMEWg{txO NHTObJUzOjR4NE[4NS=>
CHO-K1 cells MoX3SpVv[3Srb36gZZN{[Xl? NVj4eYdFOjRiaB?= MUnUdoFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDv[kBGWmKndHGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUOuOoUuODVizszN NFfqWmQzOjZ2N{KxOy=>
T47D cells NH\ZcVFHfW6ldHnvckBie3OjeR?= MV;B[49vcXO2IHXm[oVkfCCxbjD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDpckBVPDeGIHPlcIx{KGW6cILld5NqdmdiZYP0do9o\W5icnXj[ZB1d3JiYXzwbIEtKEWFNUC9O4UuODVizszN NYTLXml1OTJ2NUmwNVc>
human HEC1 cells Ml\QSpVv[3Srb36gZZN{[Xl? NEO1NHMzPCCq Mm\NRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDmeYxtNWynbnf0bEBGWmGucHjhJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUNzIHPlcIx{KGOxLXX4dJJme3OrbnegLGVTTSl{LYDTNk1tfWNiZ3Xu[UBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjZoTldkAzPCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTmnLUC1JO69VQ>? Mnn6NlE1QDF2OUe=
human SK-BR-3 cells Mn3tSpVv[3Srb36gZZN{[Xl? MknXOFghcA>? MUTJcohq[mm2aX;uJI9nKGOqZX3vZZR1emGldHHueE1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIGPLMWJTNTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJRz[W6|d3XscEBucWe{YYTpc44h[XO|YYmsJGlEPTB;MD6xJI5O M2Hz[lE3PTJyN{Oz
UAS cells M{LVUGZ2dmO2aX;uJIF{e2G7 NYHKcol4OThiaB?= MUXB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDlfJBz\XO|ZXSgbY4hXUGVIHPlcIx{KGOxLXX4dJJme3OrbnegZoV1[S2uYXP0ZY1ie2ViYX\0[ZIhOThiaILzJIJ6KGKndHGtcIFkfGGvYYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4zPSCwTR?= M3K4fFIyQDh3Mke5
COS7 cells NGD1eXdHfW6ldHnvckBie3OjeR?= NUD2OodbTGm|cHzhZ4Vu\W62IH;mJGUzNUGuZYjhOlM{KG[{b32gS2ZRNXSjZ3fl[EBGWmGucHjhJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHL5JGZCS1NuIFvpQVAvOyCwTR?= NHuyPJIyPjV{MEezNy=>
AH109 cells M1HxSWZ2dmO2aX;uJIF{e2G7 NYLnSGNwOjRiaB?= MoflRYdwdmm|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJJJm[2WydH;yJGxDTCCneIDy[ZN{\WRiaX6gfYVie3RiQVixNFkh[2WubIOgZZN{\XO|ZXSgZZMhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCHQ{WwQVEvODVibl2= Mor4NlI3PDd{MUe=
HepG2 cells NGPGWpNHfW6ldHnvckBie3OjeR?= MUSyOEBp MXTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:Oi5{IH7N M37ISlIzOjh|M{K4
COS-1 cells M2ThZWZ2dmO2aX;uJIF{e2G7 MnHRTY4hfmm2cn:gZYdwdmm|dDDhZ5Rqfmm2eTDmc5Ih\XO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGg[ZhxemW|c3XkJIlvKEORUz2xJINmdGy|LDDFR|UxRTJwNDDuUS=> NX\0ZoY2OTV5MUO0NFc>
SAOS-2 cells Ml7TSpVv[3Srb36gZZN{[Xl? Mmm5SpVv[3Srb37hcEBGe3S{b3flckBz\WOncITvdkBj\XSjIHHjeIl3cXS7IHL5JI1mfGGubH;0bIlwdmWrbjDpcoR2[3Srb36gbY4hW0GRUz2yJINmdGy|LDDFSFUxRTRwNzDuUS=> NV7WO2FEOTJ6MkSwOFM>
MDA-MB-231 cells NYK2e5VnWHKxbHnm[ZJifGmxbjDhd5NigQ>? NVzHU49GOiCmYYnz NYfFfGlWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcHWvYX6gSXIudmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCyJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUOzMlQ3KM7:TR?= MVWyOVIzOjh5Nh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.25 mM)
Water Insoluble
Ethanol '5 mg/mL

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Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage powder
in solvent
Synonyms 17β-estradiol
Smiles CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04308486 Not yet recruiting -- Non-obstructive Azoospermia Assiut University December 2020 --
NCT04189133 Not yet recruiting Drug: Lutropin alfa|Drug: Human chorionic gonadotropin Acquired Hypogonadotropic Hypogonadism Azienda Ospedaliero-Universitaria di Modena October 1 2020 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID