Estradiol

For research use only.

Catalog No.S1709 Synonyms: 17β-estradiol

4 publications

Estradiol Chemical Structure

Molecular Weight(MW): 272.38

Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone.

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10mM (1mL in DMSO) USD 270 In stock
USD 97 In stock
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Biological Activity

Description Estradiol, or more precisely, 17β-estradiol, is a human sex hormone and steroid, and the primary female sex hormone.
Targets
Estrogen receptor [1]
In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. [1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. [2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF-7 cells MoTPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\1S5Jwf3SqIILld5BwdnOnIHnuJIN2dHS3cnXzJI9nKE2FRj23JEhpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\SliY3XscJMtKEWFNUC9NU42OmVvME[g{txO NVz4XXNVQTN3N{WzNy=>
human MCF7 cells MoLESpVv[3Srb36gZZN{[Xl? MX:2JIRigXN? NULIZ5htTXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJINmdGxicILvcIln\XKjdHnvckBi\nSncjC2JIRigXNiYomgW3NVQCCjc4PhfUwhTUN3ME2zMldmNTB4IN88US=> NGjOV4UyQDd4ME[wNy=>
human U2-OS cells MXjGeY5kfGmxbjDhd5NigQ>? MUfBZ5RqfmG2aX;uJI9nKGi3bXHuJGVT[WyyaHGg[ZhxemW|c3XkJIlvKGi3bXHuJHUzNU:VIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUBz\WyjdHn2[UB1dyC3boTy[YF1\WRiY3;ueJJwdCxiRVO1NF01\S1yNjFOwG0> MoCxNlMxPDN{NEK=
MCF7 cells NFvJZ2VRem:uaX\ldoF1cW:wIHHzd4F6 MWi3NkBp MnrCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbj3k[YZq[2mnboSgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTZwNHWtNFYh|ryP MUSyNlQ3PDZ6MR?=
HeLa cells Mn3PSpVv[3Srb36gZZN{[Xl? MlL0RYN1cX[jdHnvckBw\iCnc4Tyc4dmdiC{ZYPwc45{\SCnbHXt[Y51KGmwIFjlUIEh[2WubIOgd5Ri[my7IITyZY5{\mWldHXkJJdqfGhiaIXtZY4hTXO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGsJGVEPTB;MnWtNFUh|ryP MYOxNVkxPjJ6MB?=
HEK293 cells M2rYUWZ2dmO2aX;uJIF{e2G7 MX3B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9Nk41\S1yNTFOwG0> M{DZVFI2OzB3Nki4
human Ishikawa cells NHHvO3BHfW6ldHnvckBie3OjeR?= MV23NkBp NVTp[GNKTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDld5Rzd2enbjDy[YNmeHSxcjDpckBpfW2jbjDJd4hqc2G5YTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WytYXzpcoUheGixc4DoZZRie2ViZYjwdoV{e2mxbjDh[pRmeiB5MjDodpMh[nlibXnjdo9xdGG2ZTDy[YFl\XJuIFXDOVA:Oi52NnWtNFUh|ryP M4PxWFIzPDZ2Nkix
CHO-K1 cells NV3nW2c4TnWwY4Tpc44h[XO|YYm= NHX5THAzPCCq MXnUdoFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDv[kBGWmKndHGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUOuOoUuODVizszN Mn3ZNlI3PDd{MUe=
T47D cells MmrXSpVv[3Srb36gZZN{[Xl? M4W0cWFod26rc4Sg[YZn\WO2IH;uJJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJIlvKFR2N1SgZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[SxiRVO1NF04\S1yNTFOwG0> NEjheYMyOjR3OUCxOy=>
human HEC1 cells NEjBOIVHfW6ldHnvckBie3OjeR?= M2nSRVI1KGh? NGTRcXhC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJIZ2dGxvbHXu[5RpKEWUYXzwbIEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIHj1cYFvKEiHQ{GgZ4VtdHNiY3:t[ZhxemW|c3nu[{ApTVKHKUKtdHMzNWy3YzDn[Y5mKGG|c3Xzd4VlKGG|IITyZY5{[3KrcITpc45idCCjY4TpeoF1cW:wIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;OXWtNFUh|ryP MVWyNVQ5OTR7Nx?=
human SK-BR-3 cells Mn36SpVv[3Srb36gZZN{[Xl? NYDsfXU4PDhiaB?= MWrJcohq[mm2aX;uJI9nKGOqZX3vZZR1emGldHHueE1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIGPLMWJTNTNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJRz[W6|d3XscEBucWe{YYTpc44h[XO|YYmsJGlEPTB;MD6xJI5O NFHlc3YyPjV{MEezNy=>
UAS cells NHrUOIhHfW6ldHnvckBie3OjeR?= NVPzN45bOThiaB?= NUX1fVdZSWexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEh\XiycnXzd4VlKGmwIGXBV{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGKndHGtcIFkfGGvYYPlJIFnfGW{IEG4JIhzeyCkeTDi[ZRiNWyjY4ThcYF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxRTBwMkWgcm0> NYjJZXpCOjF6OEWyO|k>
COS7 cells NFHZW41HfW6ldHnvckBie3OjeR?= NYr5Und5TGm|cHzhZ4Vu\W62IH;mJGUzNUGuZYjhOlM{KG[{b32gS2ZRNXSjZ3fl[EBGWmGucHjhJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHL5JGZCS1NuIFvpQVAvOyCwTR?= NY\NZVQ4OTZ3MkC3N|M>
AH109 cells NH7nd3NHfW6ldHnvckBie3OjeR?= MXqyOEBp NHHpe5NC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhVEKGIHX4dJJme3OnZDDpckB6\WG|dDDBTFExQSClZXzsd{Bie3Onc4Pl[EBieyCrboTldoFkfGmxbjD3bZRpKFOUQ{GgZYZ1\XJiMkSgbJJ{KGK7IHHsdIhiNWejbHHjeI9{cWSjc3WgZZN{[XluIFXDOVA:OS5yNTDuUS=> MXWyNlY1PzJzNx?=
HepG2 cells M4PGOmZ2dmO2aX;uJIF{e2G7 NUniZZc5OjRiaB?= NEK5O2JC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGg[ZhxemW|c3XkJIlvKEincFeyJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;Mj6yJI5O M1rTd|IzOjh|M{K4
COS-1 cells M3PaT2Z2dmO2aX;uJIF{e2G7 MoruTY4hfmm2cn:gZYdwdmm|dDDhZ5Rqfmm2eTDmc5Ih\XO2cn;n[Y4hemWlZYD0c5Ih[WyyaHGg[ZhxemW|c3XkJIlvKEORUz2xJINmdGy|LDDFR|UxRTJwNDDuUS=> MkO5NVU4OTN2MEe=
SAOS-2 cells NYjRVoF3TnWwY4Tpc44h[XO|YYm= NV\xZpk4TnWwY4Tpc45idCCHc4Tyc4dmdiC{ZXPldJRweiCkZYThJIFkfGm4aYT5JIJ6KG2ndHHscI91cGmxbnXpckBqdmS3Y4Tpc44hcW5iU1HPV{0zKGOnbHzzMEBGTDVyPUSuO{BvVQ>? MVWxNlgzPDB2Mx?=
MDA-MB-231 cells NGn1Wm5Rem:uaX\ldoF1cW:wIHHzd4F6 MViyJIRigXN? M{K1TGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIh2dWGwIFXSMY5m\2G2aY\lJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiMjDkZZl{KGK7IF3UWEBie3OjeTygTWM2OD1|Mz60OkDPxE1? M1fpNVI2OjJ{OEe2

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3 ; 

PubMed: 19360103     


(A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. 

β-catenin; 

PubMed: 19360103     


(B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). 

Survivin / ER-alpha / NgBR ; 

PubMed: 24223763     


MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. 

ERα / FOXP1 / KRAS / PTEN ; 

PubMed: 27441287     


Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol. 

19360103 24223763 27441287
In vivo Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. [4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. [5]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (198.25 mM)
Ethanol 5 mg/mL (18.35 mM)
Water Insoluble

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Chemical Information

Molecular Weight 272.38
Formula

C18H24O2

CAS No. 50-28-2
Storage powder
in solvent
Synonyms 17β-estradiol
Smiles CC12CCC3C(CCC4=C3C=CC(=C4)O)C1CCC2O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04308486 Not yet recruiting -- Non-obstructive Azoospermia Assiut University December 2020 --
NCT03680053 Not yet recruiting Drug: Duphaston|Drug: Cetrorelix Infertility|ART ShangHai Ji Ai Genetics & IVF Institute September 2020 Not Applicable
NCT03557619 Recruiting Drug: Venetoclax|Drug: ethinyl estradiol/levonorgestrel Hematologic Malignancies AbbVie August 28 2020 Phase 1

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Estrogen/progestogen Receptor Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID