Estradiol

Name: Estradiol
Brand: Selleck Chemicals
SKU: S1709
Rating: 5 (10 reviews)

For research use only.

Catalog No.S1709 Synonyms: 17β-estradiol, β-Estradiol, E2, 17β-Oestradiol

10 publications

CAS No. 50-28-2

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.

Selleck's Estradiol has been cited by 10 publications

Cancer Lett, 2021, 513:36-49

PubMed: 33991616 ( click the link to review the publication )

J Steroid Biochem Mol Biol, 2021, 209:105826

PubMed: 33581253 ( click the link to review the publication )

Theranostics, 2020, 10(4):1833-1848

PubMed: 32042339 ( click the link to review the publication )

Life Sci, 2020, 1;248:117449

PubMed: 32088212 ( click the link to review the publication )

BMC Cancer, 2020, 20(1):1020

PubMed: 33087072 ( click the link to review the publication )

Int J Med Sci, 2020, 17(18):3174-3189

PubMed: 33173437 ( click the link to review the publication )

Mol Cell, 2019, 75(6):1103-1116.e9

PubMed: 31420216 ( click the link to review the publication )

J Anim Sci Biotechnol, 2019, 10:34

PubMed: 31049198 ( click the link to review the publication )

Biol Blood Marrow Transplant, 2019, 25(7):1387-1394

PubMed: 30935966 ( click the link to review the publication )

Mol Med Rep, 2016, 14(6):5304-5310

PubMed: 27779689 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description

Targets

Estrogen receptor ^[1]

In vitro

Estradiol induces new dendritic spines and synapses on hippocampal CA1 pyramidal cells. Estradiol treatment resulted in a 46% increase in NMDA receptor binding. Estradiol treatment increases NMDA receptor binding in parallel with dendritic spine and synapse density. Estradiol treatment results in increased sensitivity of CA1 pyramidal cells to NMDA receptor-mediated synaptic input and that this increase is well correlated with the estradiol-induced increase in dendritic spine density in the apical dendritic tree of these cells. ^[1] 17 beta-estradiol is found to reduce Ba2+ entry reversibly via Ca2+ channels in acutely dissociated and cultured neostriatal neurons. 17 alpha-Estradiol also reduces Ba2+ currents but is significantly less effective than 17 beta-estradiol in rat neostriatal neurons. ^[2] 17 beta-estradiol exerts a dose-dependent inhibition of IL-1-, TNF-, and IL-1 and TNF-induced production of bioassayable IL-6. Estradiol inhibits both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
MCF-7	NXT4bo9mTnWwY4Tpc44h[XO\|YYm=			NUjLWotjTGm\|cHzhZ4Vu\W62IH;mJHs{UF2nc4TyZYRqd2xiZoLvcUBGe3S{b3flckBz\WOncITvdkBqdiCPQ1[tO{Bk\WyuczygTWM2OD1yLkCwN:69VQ>?	M37nV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEi2PFMoRjF3NEi2PFM9N2F-
MCF-7	M4jaTGZ2dmO2aX;uJIF{e2G7			MXTF[oZm[3SrdnWg[I9{\SCob4KgX\|NJZS1iZYP0doFlcW:uIHHnZYlve3RicILvcIln\XKjdHnvckBw\iCPQ1[tO{Bk\WyuczygSWM2OD1yLkCwNFAz\|ryP	NYKwNnU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PVY5OSd-Mke2PVY5OTxxYU6=
Ishikawa cells	MXLGeY5kfGmxbjDhd5NigQ>?			NXPVbWI5S2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBz\XG3aYLl[EB1dyCrbnT1Z4UhPTBiJTDv[kB1cGVibXH4bY12dSC\|dHnteYxifGmxbjDv[kBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSCrbjDJd4hqc2G5YTDj[YxteyxiRVO1NF0xNjByMECx{txO	NUC1OmJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOEKwNVU5Pyd-OEKwNVU5PzxxYU6=
MCF-7	NFfKNmJHfW6ldHnvckBie3OjeR?=			NI\k[GNKdmS3Y4Tpc44hd2ZicGOyJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDNR2YuPyCvYX3tZZJ6KGOjcnPpco9u[SClZXzsd{whTUN3ME2wMlAxODB7OUdOwG0>	NUL5cVVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOE[yO\|YyPSd-OE[yO\|YyPTxxYU6=
MCF-7	MlHySpVv[3Srb36gZZN{[Xl?			MUXJcoR2[3Srb36gc4YheFN{IH3SUmEh\XiycnXzd4lwdiCrbjDoeY1idiCPQ1[tO{Bu[W2vYYL5JINiemOrbn;tZUBk\WyuczygSWM2OD1yLkCwNFI6Os7:TR?=	NHPiboE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NkK3OlE2Lz56NkK3OlE2RC:jPh?=
mammary cancer cells	NHXLbpFHfW6ldHnvckBie3OjeR?=			MXXB[oZqdmm2eTDld5Rzd2enbjDy[YNmeHSxcjDv[kBOS0ZvNzDoeY1idiCvYX3tZZJ6KGOjbnPldkBk\WyuczygSWM2OD1yLkCwN\|jPxE1?	MnTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDZ{N{[xOUc,QDZ{N{[xOVww[T5?
HeLa	MWnGeY5kfGmxbjDhd5NigQ>?			MUjUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBGOjF5YnX0ZWchfXC2YXvlJIlvKE:jdICxMYV5eHKnc4PpcochUGWOYTDj[YxteyxiS3m9OE44\|ryP	MkTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDd5OUi5OEc,QDd5OUi5OFww[T5?
MCF-7	NYSxbIFxTnWwY4Tpc44h[XO\|YYm=			NGrHcmpIem:5dHigdoV{eG:wc3WgbY4h[3WudIXy[ZMhd2ZiTVPGMVchMGi3bXHuJIJz\WG\|dDDjZY5k\XJiY3XscEBtcW6nKTDj[YxteyxiRVO1NF0xNjByMECwNVXPxE1?	NFLXdIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97M{W3OVM{Lz57M{W3OVM{RC:jPh?=
MCF-7	NUK1WlltTnWwY4Tpc44h[XO\|YYm=			NIG4SW1KdmS3Y4Tpc44hd2ZicGOyJGdmdmViZYjwdoV{e2mxbjDpckBpfW2jbjDNR2YuPyClZXzsd{whTUN3ME2wMlAxODB\|MEJOwG0>	M4fSdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl\|N{GyOFEoRjl\|N{GyOFE9N2F-
MCF-7	MkPzSpVv[3Srb36gZZN{[Xl?			NVL4bIlsSW[oaX7peJkh\m:{IHXzeJJw\2WwIILlZ4VxfG:{IH;mJIh2dWGwIF3DSk04KGOnbHzzMEBGSzVyPUCuNFAxOTkQvF2=	NGfiV5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97M{exNlQyLz57M{exNlQyRC:jPh?=
HRPE	MVTGeY5kfGmxbjDhd5NigQ>?			MkKxWHBgXFKDTmPQU3JVTVJ8IHnubIljcXSrb36gc4YhXEWDIIXweIFs\SBqVFXBPkAzOCC3TTmgbY4hV0OWMz3lfJBz\XO\|aX7nJGhTWEViY3XscJMtKEmFNUC9NU4y\|ryP	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDNyMEKyK\|46QDNyMEKyQE9iRg>?
MCF-7	M1XIO2Z2dmO2aX;uJIF{e2G7			NUHLXWQySWexbnnzeEBm\m[nY4Sgc44hfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hcW5iTVPGMVch[2WubIOg[ZhxemW\|c3nu[{Bme3S{b3flckBz\WOncITvdkBidHCqYTygSWM2OD1yLkCwNFAxP87:TR?=	NXPsfGdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC2O\|MxQTlpPkGwOlc{ODl7PD;hQi=>
HeLa	M4XRc2Z2dmO2aX;uJIF{e2G7			M2L0bWFkfGm4YYTpc44hd2ZiZYP0do9o\W5icnXzdI9ve2ViZXzlcYVvfCCrbjDI[WxiKGOnbHzzJJN1[WKueTD0doFve2[nY4Tl[EB4cXSqIHj1cYFvKEW\|dILv[4VvKHKnY3XweI9zKGGucHjhMkwhTUN3ME2wMlAxODB{zszN	MlTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7ME[yPFAoRjFzOUC2NlgxRC:jPh?=
HeLa	NV;jTnBJTnWwY4Tpc44h[XO\|YYm=			NFW0d5ZC[3SrdnH0bY9vKG:oIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRiaX6gTIVN[SClZXzsd{B{fGGkbImgeJJidnOoZXP0[YQhf2m2aDDoeY1idiCHc4Tyc4dmdiC{ZXPldJRweiCkZYThMkwhTUN3ME2wMlAxODB5zszN	NV;NVWJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
SH-SY5Y	M1jsWGZ2dmO2aX;uJIF{e2G7			Ml7TRYdwdmm\|dDDhZ5Rqfmm2eTDpckB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBie3OjeTDpckBUUC2VWUXZJI5mfXKxYnzhd5RwdWFiY3XscJMh\XiycnXzd4lv\yCHc4Tyc4dmdiC{ZXPldJRweiCkZYThMEBGSzVyPUCuNFAxOc7:TR?=	NXT6VnNERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5NFYzQDBpPkGxPVA3OjhyPD;hQi=>
HEK293	Mo\FSpVv[3Srb36gZZN{[Xl?			NXT5fIZoXFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiTWDQL{B2eHSja3WgLG1RWCt8IECuNlUhfU1rIHnuJG9EXDNvZYjwdoV{e2mwZzDISWszQTNiY3XscJMtKEmFNUC9Nk45QM7:TR?=	MkXZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzMUC2NFcoRjF{MUGwOlA4RC:jPh?=
HEK293	NX:0ZYFkTnWwY4Tpc44h[XO\|YYm=			MoLBWHBgXFKDTmPQU3JVTVJ8IHnubIljcXSrb36gc4YhVVCSKzD1dJRic2ViKF3QVEs7KDBwMkWgeW0qKGmwIF;DWFEu\XiycnXzd4lv\yCKRVuyPVMh[2WubIOsJGlEPTB;NT63N:69VQ>?	MojRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzMUC2NFcoRjF{MUGwOlA4RC:jPh?=
MCF-7-2a	M1PFN2Z2dmO2aX;uJIF{e2G7			NHPHbpJC\2:waYP0JIFkfGm4aYT5JIlvKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGG\|c3H5JIlvKE2FRj23MVJiKGOnbHzzJINwdXCjcnXkJJRwKGW\|dILh[IlwdCCHMjygSWM2OD1yLkCwNFQ6\|ryP	NYfCNXAyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKxN\|k1PDdpPkGyNVM6PDR5PD;hQi=>
T47D	M4X4ZmZ2dmO2aX;uJIF{e2G7			M1rvR2Fod26rc4Sg[YZn\WO2IH;uJJRz[W6\|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJIlvKFR2N1SgZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[SxiRVO1NF0xNjByMEC3{txO	NUDSVW9yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0OVkxOTdpPkGyOFU6ODF5PD;hQi=>
T47D	MojPSpVv[3Srb36gZZN{[Xl?			M3LrdGFod26rc4Sg[YZn\WO2IH;uJJRz[W6\|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJI9nKFR2N1SgZ4VtdHNiZYjwdoV{e2mwZzDoeY1idiCnc4Tyc4dmdiC{ZXPldJRweiCkZYThMEBGSzVyPUCuNFAxQM7:TR?=	NIDKSHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkS1PVAyPyd-MUK0OVkxOTd:L3G+
MCF-7	M33KTGZ2dmO2aX;uJIF{e2G7			Mn;vVIVz[2WwdDDh[49vcXO2aXOgZYN1cX[rdImg[o9zKGW\|dILv[4VvNWmwZIXj[YQheFN{IHX4dJJme3Orb36gbY4hVUOILUegZ4VtdHNuIFXDOVA:OC5yMECwNe69VQ>?	Ml\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ5NEm4PVgoRjF{N{S5PFk5RC:jPh?=
MCF-7	MV7GeY5kfGmxbjDhd5NigQ>?			MV;GeY5kfGmxbnHsJGVT[WyyaHGgdoVk\XC2b4KgZYN1cX[rdImgbY4hfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[XO\|YYmgbY4hVUOILUegZ4VtdHNuIFXEOVA:OC5yMECwNFQ3\|ryP	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NEC0N{c,OTJ6MkSwOFM9N2F-
SAOS-2	NHH0TnNHfW6ldHnvckBie3OjeR?=			M1njOmZ2dmO2aX;uZYwhTXO2cn;n[Y4hemWlZYD0c5Ih[mW2YTDhZ5Rqfmm2eTDifUBu\XSjbHzveIhqd26naX6gbY5lfWO2aX;uJIlvKFODT2OtNkBk\WyuczygSWQ2OD1yLkCwOFfPxE1?	Ml\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6MkSwOFMoRjF{OEK0NFQ{RC:jPh?=
MCF-7 breast tumor	MXLGeY5kfGmxbjDhd5NigQ>?			NFHQWZhG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDh[4FqdnO2IF3DSk04KGK{ZXHzeEB1fW2xcjDj[YxteyC3c3nu[{BOS0ZvNzDhd5NigS5uIFXDOVA:OC5yMECwNe69VQ>?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NUmzOUc,OTJ6MkW5N\|U9N2F-
MCF-7-2a	M17YVWZ2dmO2aX;uJIF{e2G7			MmDDR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDhZ5RqfmG2ZTDseYNq\mW{YYPlJIV5eHKnc4Ppc44hcW5iTVPGMVcuOmFiY3XscJMtKEWFNUC9NE4xODByON88US=>	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd4MUG5N{c,OTR5NkGxPVM9N2F-
Ishikawa Var-1 cells	MU\GeY5kfGmxbjDhd5NigQ>?		M3rCZVczKGi{cx?=	NUTIU4wxTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDld5Rzd2enbjDy[YNmeHSxcjDpckBpfW2jbjDJd4hqc2G5YTDWZZIuOSClZXzsd{Bie3Onc4Pl[EBieyC\|dHnteYxifGmxbjDv[kBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSCvZXHzeZJm\CCkeTDt[ZRi[m:uaYPtJI9nKHBvbnn0do9xcGWwb3ygdIhwe3CqYYThd4Uh[W[2ZYKgO\|IhcHK\|LDDFR\|UxRTBwMECwNFQ4\|ryP	NVTzW4plRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1Olg4PzBpPkG1OVY5PzdyPD;hQi=>
HeLa	M3Lx[2Z2dmO2aX;uJIF{e2G7			NIi2V5hKdmirYnn0bY9vKG:oIGuzTH1me3S{YXTpc4wh[mmwZHnu[{B1dyCqdX3hckBme3S{b3flckBz\WOncITvdkBj\XSjIHX4dJJme3OnZDDpckBJ\UyjIHPlcIx{NCCLQ{WwQVAvODJ2zszN	M1\mUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFUyLz5zNU[1PFg2OTxxYU6=
HeLa	NWrLPI5GTnWwY4Tpc44h[XO\|YYm=			MkD5TY5pcWKrdHnvckBw\iCdM1jd[ZN1emGmaX;sJIJqdmSrbnegeI8hcHWvYX6g[ZN1em:pZX6gdoVk\XC2b4KgZYxxcGFiZYjwdoV{e2WmIHnuJGhmVGFiY3XscJMtKEmFNUC9NE4xOjkQvF2=	NIPvSFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[1PFg2OSd-MUW2OVg5PTF:L3G+
COS-1	MkW1SpVv[3Srb36gZZN{[Xl?			M1\NSWlvKH[rdILvJIFod26rc4SgZYN1cX[rdImg[o9zKGW\|dILv[4VvKHKnY3XweI9zKGGucHjhJIV5eHKnc4Pl[EBqdiCFT2OtNUBk\WyuczygSWM2OD1yLkCwNlTPxE1?	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzM{SwO{c,OTV5MUO0NFc9N2F-
COS-1	NWn6O\|VwTnWwY4Tpc44h[XO\|YYm=			NUjQSGpKUW5idnn0do8h[WexbnnzeEBi[3Srdnn0fUBnd3JiZYP0do9o\W5icnXj[ZB1d3JiYnX0ZUBmgHC{ZYPz[YQhcW5iQ1;TMVEh[2WubIOsJGVEPTB;MD6wNFk4\|ryP	M3XSPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{GzOFA4Lz5zNUexN\|QxPzxxYU6=
293T	MWDGeY5kfGmxbjDhd5NigQ>?			M4\1UmRqe3CuYXPlcYVvfCCxZjDbN2heOTekZYThMYV{fHKjZHnvcEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJFI6O1RiY3XscJMtKEmFNUC9NE4xODB5Nd88US=>	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJzOUS2N{c,OTZ{MUm0OlM9N2F-
293T	NH73ZnVHfW6ldHnvckBie3OjeR?=			NX62O\|BlTGm\|cHzhZ4Vu\W62IH;mJHs{UF1zN3LleIEu\XO2cnHkbY9tKG[{b32gdoVkd22kaX7hcpQhcHWvYX6gSXJj\XSjIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwQVAvODBzMUlOwG0>	NIKwbpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkKxPVQ3Oyd-MU[yNVk1PjN:L3G+
HEK293	Ml\CSpVv[3Srb36gZZN{[Xl?			Mo\MSYZn\WO2IH;uJJRz[W6\|YXP0bZZifGmxbjDv[kBCVFBiZ3Xu[UBmgHC{ZYPzbY9vKGmwIFjFT\|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIhGWmGucHjhMEBGSzVyPUCuNFAxPzYQvF2=	NEG0dIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkOwPVkxPyd-MU[zNFk6ODd:L3G+
HEK293	M1y3c2Z2dmO2aX;uJIF{e2G7			MlrHSYZn\WO2IH;uJJRz[W6\|YXP0bZZifGmxbjDv[kBCVFBiZ3Xu[UBmgHC{ZYPzbY9vKGmwIFjFT\|I6OyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIhGWmKndHGsJGVEPTB;MD6wNFIy\|ryP	M3;p[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{C5PVA4Lz5zNkOwPVkxPzxxYU6=
SK-BR-3	MYfGeY5kfGmxbjDhd5NigQ>?		MnHyOFghcHK\|	MY\Jcohq[mm2aX;uJI9nKGOqZX3vZZR1emGldHHueE1qdmS3Y3XkJINmdGxibXnndoF1cW:wIH;mJIh2dWGwIGPLMWJTNTNiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JJRz[W6\|d3XscEBucWe{YYTpc44h[XO\|YYmsJGlEPTB;MD6wNFAy\|ryP	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N\|M9N2F-
COS7	M4[3TmZ2dmO2aX;uJIF{e2G7			MkHhSIl{eGyjY3Xt[Y51KG:oIFWyMWFt\XijNkOzJIZzd21iR1\QMZRi\2enZDDFVoFteGijIHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGK7IF\BR3MtKEurPUCuNFAxO87:TR?=	M1TOWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwO\|M{Lz5zNkWyNFc{OzxxYU6=
COS7	MnfvSpVv[3Srb36gZZN{[Xl?			NInsNIdC\2:waYP0JIFkfGm4aYT5JIF1KEeIUD30ZYdo\WRiR2DSN\|Ah\XiycnXzd4VlKGmwIFPPV\|ch[2WubIOgZZN{\XO\|ZXSgZZMhcGGuZjDsbYZmKG[xcjDpcoNz\WG\|ZTDpckBqdnS{YXPlcIx2dGG{IHPhcINqfW1ibHX2[Ywh[nlic4DlZ5Rzd2[udX;ybY1mfHK7LDDFR\|UxRTBwMECwN:69VQ>?	NID3W3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWyNFc{Oyd-MU[1NlA4OzN:L3G+
COS7	NFLuOHFHfW6ldHnvckBie3OjeR?=			MXjEbZNxdGGlZX3lcpQhd2ZiRUKtRYxmgGF4M{Og[pJwdSCJRmCteIFo\2WmIFXSZoV1[SCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{BjgSCIQVPTMEBMcT1yLkCwNFM5\|ryP	MojBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N\|MoRjF4NUKwO\|M{RC:jPh?=
MCF7	NILBeWhHfW6ldHnvckBie3OjeR?=		MYi0PEBpenN?	Mn\nTY5pcWKrdHnvckBw\iClaHXtc4F1fHKjY4ThcpQucW6mdXPl[EBk\WyuIH3p[5JifGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTD0doFve3enbHygcYloemG2aX;uJIF{e2G7LDDJR\|UxRTBwMECwOO69VQ>?	NFLBRZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWyNFc{Oyd-MU[1NlA4OzN:L3G+
COS7	MmW2SpVv[3Srb36gZZN{[Xl?			MXjEbZNxdGGlZX3lcpQhd2ZiRUKtRYxmgGF4M{Og[pJwdSCJRmCteIFo\2WmIFfQVlMxKGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIJ6KE[DQ2OsJGtqRTBwMEC1O:69VQ>?	Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N\|MoRjF4NUKwO\|M{RC:jPh?=
COS7	M1X0b2Z2dmO2aX;uJIF{e2G7	M{XYelEhdk1?		NF7NW4lC\2:waYP0JIFkfGm4aYT5JIF1KEeIUD30ZYdo\WRiRWLhcJBp[SCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXygZZQhOSCwTTDifUB{eGWldILv[ox2d3KrbXX0dpk>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N\|M9N2F-
COS7	M3\iXmZ2dmO2aX;uJIF{e2G7	MVmxJI5O		NGnSUXZC\2:waYP0JIFkfGm4aYT5JIF1KEeIUD30ZYdo\WRiRWLi[ZRiKGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh[2GuY3n1cUBt\X[nbDDheEAyKG6PIHL5JJNx\WO2cn;mcJVwemmvZYTyfS=>	NEG1ZWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWyNFc{Oyd-MU[1NlA4OzN:L3G+
COS7	NXnBOGJbTnWwY4Tpc44h[XO\|YYm=	MmTmNUBvVQ>?		NFvwSGpC\2:waYP0JIFkfGm4aYT5JIF1KEeIUD30ZYdo\WRiRWLhcJBp[SCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bie3Onc4Pl[EBieyCSSUPLJIFkfGm4YYTpc44h[XRiMTDuUUBjgSCSSWCzJI52[2ynYYKgZYNkfW23bHH0bY9vKGG\|c3H5	M136UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUKwO\|M{Lz5zNkWyNFc{OzxxYU6=
COS7	MmmwSpVv[3Srb36gZZN{[Xl?	NFzTSXgyKHWP		MWXB[49vcXO2IHHjeIl3cXS7IHH0JGdHWC22YXfn[YQhTVKkZYThJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy\|IHHzd4V{e2WmIHHzJHBKO0tiYXP0bZZifGmxbjDheEAyKG6PIHL5JHBKWDNiboXjcIVieiCjY3P1cZVt[XSrb36gZZN{[Xl?	MkLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N\|MoRjF4NUKwO\|M{RC:jPh?=
COS7	MmXVSpVv[3Srb36gZZN{[Xl?	MnfvNUB2VQ>?		NFjlVW9C\2:waYP0JIFkfGm4aYT5JIF1KEeIUD30ZYdo\WRiR2DSN\|Ah\XiycnXzd4VlKGmwIFPPV\|ch[2WubIOgZZN{\XO\|ZXSgZZMhWEl\|SzDhZ5RqfmG2aX;uJIF1KDFibl2gZpkhWEmSMzDueYNt\WG{IHHjZ5VufWyjdHnvckBie3OjeR?=	Ml\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N\|MoRjF4NUKwO\|M{RC:jPh?=
COS7	MWPGeY5kfGmxbjDhd5NigQ>?	NUm1WpRLOSCwTR?=		NHjqeZlDcW6maX7nJIFn\mmwaYT5JJRwKG2URmCxMZRi\2enZDDHVHI{OCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bkd2W6cILld5NqdmdiR1\QMZRi\2enZDDFVoFteGijIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiRUKtRYxmgGF4M{OgZolv\GmwZzDpckBvfWOuZYXzJIF1KDFidV2gZpkhTkGFUx?=	Ml;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3MkC3N\|MoRjF4NUKwO\|M{RC:jPh?=
COS7	MnjaSpVv[3Srb36gZZN{[Xl?	M1HkVlEhdk1?		NFTKdYtDcW6maX7nJIFn\mmwaYT5JJRwKEeIUD30ZYdo\WRiRWLhcJBp[SCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bkd2W6cILld5NqdmdibWLGVFEufGGpZ3XkJGdRWjNyIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiRUKtRYxmgGF4M{OgZolv\GmwZzDpckBvfWOuZYXzJIF1KDFidV2gZpkhTkGFUx?=	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV{MEezN{c,OTZ3MkC3N\|M9N2F-
MCF7	Mkn5SpVv[3Srb36gZZN{[Xl?	NWDne2E3OSCwTR?=	NEXaXlkzPCCqcoO=	NYH3R\|NsTG:5bjDy[Yd2dGG2aX;uJI9nKGW\|dILv[4VvKHKnY3XweI9zKGW6cILld5Nqd25iaX6gUWNHPyClZXzsd{BifCBzIH7NJIFnfGW{IEK0JIhzew>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ5NUOxOUc,OTd{N{WzNVU9N2F-
HEK293	MVPGeY5kfGmxbjDhd5NigQ>?			MYrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JGVT[WyyaHGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVA:OC5yMEC3Oe69VQ>?	NVPoWGxPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OFg3PTZpPkG3OFQ5PjV4PD;hQi=>
HEK293	MYLGeY5kfGmxbjDhd5NigQ>?			MoTJRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDy[YNwdWKrbnHueEBGWmKndHGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUB1emGwc3HjeIl3[XSrb36gZZN{[XluIFXDOVA:OC5yMEKx{txO	MoH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NEi2OVYoRjF5NES4OlU3RC:jPh?=
HEK293	NIX1d3BHfW6ldHnvckBie3OjeR?=			M1K5fWFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIJ6KHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OD1yLkCwNFc2\|ryP	NYT1boJsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PVAxQDRpPkG3PFkxODh2PD;hQi=>
HEK293	MofjSpVv[3Srb36gZZN{[Xl?			M3P6T2Fod26rc4SgZYN1cX[rdImgZZQhTVKkZYThJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxRTBwMECyNe69VQ>?	Ml;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OUCwPFQoRjF5OEmwNFg1RC:jPh?=
MCF7 BUS	NETHUmFHfW6ldHnvckBie3OjeR?=			NXjZdZJ3TXO2cn;n[Y5q[yCyb4TlcoN6KGmwIHj1cYFvKE2FRkegRnVUKGOnbHzzJIF{e2W\|c3XkJIF{KGS{dXegcIV3\WxiaX7keYNqdmdibXH4bY1idCClZXzsJJBzd2yrZnXyZZRqd25uIF3FSF0xNjByMd88US=>	NEf4UJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0Nlk6Pyd-MUiyOFI6QTd:L3G+
CHO	NImyN3lHfW6ldHnvckBie3OjeR?=			MXrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGTHVlUh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDifUBtfWOrZnXyZZNmKGG\|c3H5MEBGSzVyPUO4MlTPxE1?	MmTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|MEeyPVQoRjF6M{C3Nlk1RC:jPh?=
MCF7	NYnQWpE2TnWwY4Tpc44h[XO\|YYm=		MkHEOkBl[Xm\|	NWDxXnl5TXO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJINmdGxicILvcIln\XKjdHnvckBi\nSncjC2JIRigXNiYomgW3NVQCCjc4PhfUwhTUN3ME2wMlAxODByM{hOwG0>	NHzzWZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEe2NFYxOyd-MUi3OlA3ODN:L3G+
HEC1	NU\qOFh2TnWwY4Tpc44h[XO\|YYm=		NInuNIszPCCqcoO=	MVfB[49vcXO2IHHjeIl3cXS7IHH0JIZ2dGxvbHXu[5RpKGi3bXHuJIV{fHKxZ3XuJJJm[2WydH;yJIJmfGFiZYjwdoV{e2WmIHnuJIh2dWGwIFjFR\|Eh[2WubIOgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMEGz{txO	M3zFOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK4NFE3Lz5zOUGyPFAyPjxxYU6=
HEC1	NXvVN3BGTnWwY4Tpc44h[XO\|YYm=		NGfOU4YzPCCqcoO=	MVHB[49vcXO2IHHjeIl3cXS7IHH0JIZ2dGxvbHXu[5RpKGi3bXHuJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijIHX4dJJme3OnZDDpckBpfW2jbjDISWMyKGOnbHzzJIF{e2W\|c3XkJIF{KHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEC3{txO	M2HrSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUK4NFE3Lz5zOUGyPFAyPjxxYU6=
HEC1	MnG1SpVv[3Srb36gZZN{[Xl?		MmjkNlQhcHK\|	MoP1RYdwdmm\|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUNzIHPlcIx{KGG\|c3Xzd4VlKGG\|IITyZY5{[3KrcITpc45idCCyb4TlcoN6KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmNWKndHGg[4Ft[WO2b4Pp[IF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxRTBwMECwNe69VQ>?	NUXlXlh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyPFYzQDNpPkG5Nlg3Ojh\|PD;hQi=>
HEC1	MnOxSpVv[3Srb36gZZN{[Xl?		MYSyOEBpenN?	M4nE[WFod26rc4SgZYN1cX[rdImgZZQhTVKkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUNzIHPlcIx{KGG\|c3Xzd4VlKGG\|IITyZY5{[3KrcITpc45idCCyb4TlcoN6KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmNWKndHGg[4Ft[WO2b4Pp[IF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxRTBwMECwOe69VQ>?	MknWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{OE[yPFMoRjF7Mki2Nlg{RC:jPh?=
BL21(DE3)	M3XoZ2Z2dmO2aX;uJIF{e2G7			NEPzNm5C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEpTEV\|KTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[2:jY4TpeoF1d3JiNUOtdJJwfGWrbjDibY5lcW6pIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfS=>	NUCzUoVLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1OlE3OTlpPkG5OVYyPjF7PD;hQi=>
BL21(DE3)	M{njSGZ2dmO2aX;uJIF{e2G7			M4XD[mFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJj\XSjIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxLGRGOyliY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJINw[WO2aY\heI9zKDV\|LYDyc5RmcW5iYnnu[Ilv\yCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYm=	M{Pob\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NU[xOlE6Lz5zOUW2NVYyQTxxYU6=
SF9	M4\FeGZ2dmO2aX;uJIF{e2G7			MmXFSIl{eGyjY3Xt[Y51KG:oIGuzTH0yP2KndHGt[ZN1emGmaX;sJIZzd21iaIXtZY4hTVKjbIDoZUBmgHC{ZYPz[YQhcW5iU1[5JINmdGy\|LDDJR\|UxRTBwMEGyOe69VQ>?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh\|Nkm0PUc,OTl6M{[5OFk9N2F-
COS7	NH\PPXhHfW6ldHnvckBie3OjeR?=			NUDwfWZFSWexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZUBtcWejbnSgZolv\GmwZzDkc41icW5iZYjwdoV{e2WmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KEORU{egZ4VtdHNiY3:teJJidnOoZXP0[YQhf2m2aDDHZYw1NUyERDDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEOy{txO	MmjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NkOwPFMoRjF7OE[zNFg{RC:jPh?=
COS7	M2j6eGZ2dmO2aX;uJIF{e2G7			M1rxRWFod26rc4SgZYN1cX[rdImgZZQh\XO2cn;n[Y4hemWlZYD0c5Ih[mW2YTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h\XiycnXzd4VlKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNQWzdiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCJYXy0MWxDTCCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xOTIQvF2=	MnzVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NkOwPFMoRjF7OE[zNFg{RC:jPh?=
MCF7:WS8	MX7GeY5kfGmxbjDhd5NigQ>?		MUK3JIRigXN?	MWfFd5Rzd2enbnnjJIFkfGm4aYT5JIlvKGi3bXHuJGVT[WyyaHGg[ZhxemW\|c3nu[{BOS0Z5OmfTPEBk\WyuczDhd5Nme3OnZDDhd{B{fGmvdXzheIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36gZYZ1\XJiNzDkZZl{NCCHQ{WwQVAvODByMECx{txO	MkPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB\|M{SzOlgoRjJyM{O0N\|Y5RC:jPh?=
MCF7:WS8	M2TCbmZ2dmO2aX;uJIF{e2G7			MYfBZ5RqfmG2aX;uJI9nKGi3bXHuJGVTKGmwIHj1cYFvKE2FRke6W3M5KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemW\|cH;ud4l3\SCnbHXt[Y51KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	NFvvepc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEOzOFM3QCd-MkCzN\|Q{Pjh:L3G+
T47D:C:4:2	NHvpXJZHfW6ldHnvckBie3OjeR?=			NVvGbJJbTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDD3bYxlKHS7cHWgSXIh[WyyaHGg[ZhxemW\|c3XkJIlvKFR2N1S6R\|o1QjJiY3XscJMh[2:neIDy[ZN{cW6pIHXzeJJw\2WwIILld5BwdnOnIHXs[Y1mdnRiYomg[JVmdCCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M3u2clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{O0N\|Y5Lz5{MEOzOFM3QDxxYU6=
T47D:C:4:2	NX7MUlZQTnWwY4Tpc44h[XO\|YYm=			M1rpPWV{fHKxZ3XubYMh[WO2aY\peJkh[XRid3ns[EB1gXCnIFXSJIFteGijIFSzOVFIKG23dHHueEBmgHC{ZYPz[YQhcW5iVES3SFpEQjR8MjDj[YxteyClb3X4dJJme3Orbneg[ZN1em:pZX6gdoV{eG:wc3Wg[YxmdWWwdDDifUBlfWWuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MlzYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB\|M{SzOlgoRjJyM{O0N\|Y5RC:jPh?=
HEK293	Ml3kSpVv[3Srb36gZZN{[Xl?		M{\2S\|E5KGi{cx?=	MlnOWJJidnOjY4TpeoF1cW:wIH;mJIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gTGVMOjl\|IHPlcIx{KGGodHXyJFE5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOlE4\|ryP	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODRyOEWzNkc,OjB2MEi1N\|I9N2F-
HEK293	MUfGeY5kfGmxbjDhd5NigQ>?		MYexPEBpenN?	NGHvWZhVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6gSXJidHCqYTDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFAxPjR4zszN	NGfvSI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESwPFU{Oid-MkC0NFg2OzJ:L3G+
CHO-K1	NGSxTm1HfW6ldHnvckBie3OjeR?=			NX7YRZpRSWexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEh\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxRTBwMEROwG0>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV3M{CyN{c,OjB3NUOwNlM9N2F-
CHO-K1	NImwcHJHfW6ldHnvckBie3OjeR?=			NX\0SI9VSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKHS{YX7zZYN1cX[jdHnvckBie3OjeTygSWM2OD1yLkC1{txO	Mne3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3NUOwNlMoRjJyNUWzNFI{RC:jPh?=
HeLa	MkfESpVv[3Srb36gZZN{[Xl?		Mn7XNVghcHK\|	NWH1VZRPTGm\|cHzhZ4Vu\W62IH;mJHszNDRuNjy3MVNJZWW\|dILh[IlwdCCocn;tJIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhmVGFiY3XscJMh[W[2ZYKgNVghcHK\|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaU2wMlAxODF4zszN	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ{MUS5Nkc,OjB4MkG0PVI9N2F-
HEK293T	NWXRNnk1TnWwY4Tpc44h[XO\|YYm=			MXzB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiRUO1N2EhdXW2YX70JIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJINwNWW6cILld5NqdmdiRWLFJIF{e2W\|c3XkJIF{KHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFI2\|ryP	NYHLdHhvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2OVk5ODFpPkKwOlU6QDBzPD;hQi=>
HEK293T	NGq5O\|RHfW6ldHnvckBie3OjeR?=			NEj5S5ZC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[mW2YTDFN\|A2SSCvdYThcpQh\XiycnXzd4VlKGmwIFjFT\|I6O1RiY3XscJMh[29vZYjwdoV{e2mwZzDFVmUh[XO\|ZYPz[YQh[XNidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2wMlAyODUQvF2=	NYmzbohURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2OVk5ODFpPkKwOlU6QDBzPD;hQi=>
HeLa	M4DZW2Z2dmO2aX;uJIF{e2G7			M{HVPGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6ge4lt\CC2eYDlJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmVGFiY3XscJMh[29vZYjwdoV{e2mwZzDFVmUh[XO\|ZYPz[YQh[XNidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	Mor2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4NUm4NFEoRjJyNkW5PFAyRC:jPh?=
HeLa	Ml3tSpVv[3Srb36gZZN{[Xl?			NIT4UZpVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6ge4lt\CC2eYDlJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmVGFiY3XscJMh[29vZYjwdoV{e2mwZzDBVE0yKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ3OUiwNUc,OjB4NUm4NFE9N2F-
HeLa	MULGeY5kfGmxbjDhd5NigQ>?			M4PRSGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6ge4lt\CC2eYDlJGVT[mW2YTDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{Bkdy2neIDy[ZN{cW6pIFXSSUBie3Onc4Pl[EBieyC2cnHud4FkfGm4YYTpc44h[WO2aY\peJkh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ3OUiwNUc,OjB4NUm4NFE9N2F-
HeLa	NEnicVlHfW6ldHnvckBie3OjeR?=			NV7idYhpSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiC5aXzkJJR6eGViRWLi[ZRiKGW6cILld5Nm\CCrbjDoeY1idiCKZVzhJINmdGy\|IHPvMYV5eHKnc4PpcochSVBvMTDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	M4G5dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkW5PFAyLz5{ME[1PVgxOTxxYU6=
U2OS	NHrQZmJHfW6ldHnvckBie3OjeR?=		MnnHNVghcHK\|	M1ziSmFod26rc4SgZYN1cX[rdImgZZQhTVKkZYThMWxDTCCrbjDoeY1idiCXMl;TJINmdGy\|IITyZY5{\mWldHXkJJdqfGhiR3HsOE1FSkRiYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4YhfHKjboPhZ5RqfmG2aX;uJIFkfGm4aYT5JIFnfGW{IEG4JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODB2NUix{txO	NXzZUYZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4NVI3QDFpPkKwPFEzPjhzPD;hQi=>
U2OS	NVjDRlFtTnWwY4Tpc44h[XO\|YYm=		M1nTPVE5KGi{cx?=	NUDYW4ZVSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiNUyERDDpckBpfW2jbjDVNm9UKGOnbHzzJJRz[W6\|ZnXjeIVlKHerdHigS4FtPC2GQlSgZZN{\XO\|ZXSgZZMhcW6lcnXhd4Uhd2ZidILhcpNi[3SrdnH0bY9vKGGldHn2bZR6KGGodHXyJFE5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEK3O\|PPxE1?	MmO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6MUK2PFEoRjJyOEGyOlgyRC:jPh?=
U2OS	Mn\nSpVv[3Srb36gZZN{[Xl?		NGKzdVkyQCCqcoO=	MlSxRY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnLleIEuVEKGIHnuJIh2dWGwIGWyU3Mh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDHZYw1NUSERDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCjZoTldkAyQCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR\|UxRTBwMEGwO\|TPxE1?	NEDGcVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEixNlY5OSd-MkC4NVI3QDF:L3G+
U2OS	MojBSpVv[3Srb36gZZN{[Xl?		MWOxPEBpenN?	M{jOUmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiRWLhcJBp[S2OQlSgbY4hcHWvYX6gWVJQWyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJGdidDRvRFLEJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[geJJidnOjY4TpeoF1cW:wIHHjeIl3cXS7IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGlEPTB;MD6wNlExPM7:TR?=	MkS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6MUK2PFEoRjJyOEGyOlgyRC:jPh?=
HEK293	M1vDeWZ2dmO2aX;uJIF{e2G7			M17uOWFod26rc4SgZYN1cX[rdImgZZQhYk[SLX\1d4VlKEWUUnLleIEhVEKGIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgWHIuTlKHVDDhd5NigSxiRVO1NF0xNjByNEdOwG0>	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJzOEe4N{c,OjF{MUi3PFM9N2F-
MCF7	NGTtWpdHfW6ldHnvckBie3OjeR?=		NV;F[oZHfXBidH:gOkBl[Xm\|	NUT5RoRRSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJGxDTCCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iYX\0[ZIhfXBidH:gOkBl[Xm\|IHL5JINmdGy2aYTldk1odG9iYYPzZZktKEWFNUC9NE4xOc7:TR?=	NW\JdI9tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyNVg4QDNpPkKxNlE5Pzh\|PD;hQi=>
MCF7	M3nhb2N6fG:2b4jpZ4l1gSCjc4PhfS=>	NYi2SVFKPCC3TR?=	MlraNlQhcHK\|	NX7sbm1GS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjdDC0JJVOKGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZk>	M3jDSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEW5OVgyLz5{MUS1PVU5OTxxYU6=
MCF7	MkfqSpVv[3Srb36gZZN{[Xl?	M1fod\|QhfU1?	NF[4ZogzPCCqcoO=	NEG1d\|FT\WS3Y4Tpc44hcW5iQ3TrNUBxem:2ZXnuJIxmfmWuIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhPCC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR3OUW4NUc,OjF2NUm1PFE9N2F-
HEC1	M2nu[GZ2dmO2aX;uJIF{e2G7		MXmyOEBpenN?	M3P4R2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6g[pVtdC2uZX7neIghTVKjbIDoZUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iaIXtZY4hUEWFMTDj[YxteyClbz3lfJBz\XO\|aX7nJEhGWkVrMj3wV\|IudHWlIHflcoUh[XO\|ZYPz[YQh[XNidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwNFA6\|ryP	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR6MUS5O{c,OjF2OEG0PVc9N2F-
HEC1	NHPNZXJHfW6ldHnvckBie3OjeR?=		MnHJNlQhcHK\|	MVnB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIH\1cIwudGWwZ4ToJGVT[mW2YTDy[YNmeHSxcjDlfJBz\XO\|ZXSgbY4hcHWvYX6gTGVEOSClZXzsd{Bkdy2neIDy[ZN{cW6pIDjFVmUqOi2yU{KtcJVkKGenbnWgZZN{\XO\|ZXSgZZMhfHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44h[W[2ZYKgNlQhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjByMEey{txO	NUDtdWQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0PFE1QTdpPkKxOFgyPDl5PD;hQi=>
MCF7	MWjGeY5kfGmxbjDhd5NigQ>?	M1rPWVAvODFibl2=	MX60PEBpenN?	M3jpfmV{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hTVJvcH;zbZRqfmViTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{B{\WO{ZYTl[EBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSCjdDCwMlAyKG6PIHHmeIVzKDR6IHjyd{BjgSCyaH;zdIhiNWyrZ3j0JJJmeG:{dHXyJINp\W2rbIXtbY5me2OnbnPlJIF{e2G7	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOThyMEi1PUc,OjF6MEC4OVk9N2F-
HEK	Mm\aSpVv[3Srb36gZZN{[Xl?		MVqxPEBpenN?	M4rnRmFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iSFXLJINmdGy\|IHPvMYV5eHKnc4Ppcoch[mW2YT3sZYN1[W2jc3WgZYZ1\XJiMUigbJJ{KGK7IHLleIEudGGldHHtZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOFbPxE1?	MmX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6OEWyO\|koRjJzOEi1Nlc6RC:jPh?=
UAS	Mli2SpVv[3Srb36gZZN{[Xl?		Mlr6NVghcHK\|	NIPXXo5C\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBmgHC{ZYPz[YQhcW5iVVHTJINmdGy\|IHPvMYV5eHKnc4Ppcoch[mW2YT3sZYN1[W2jc3WgZYZ1\XJiMUigbJJ{KGK7IHLleIEudGGldHHtZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECyOe69VQ>?	MlLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6OEWyO\|koRjJzOEi1Nlc6RC:jPh?=
HEC-1	NIiySWNHfW6ldHnvckBie3OjeR?=		MYOyOEBpenN?	MWPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhGSy1zIHPlcIx{KGOxZYjwdoV{e2mwZzDi[ZRiNWejbDDhd5Nme3OnZDDhd{BtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMUKz{txO	NIiwTGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGyNlU3Oyd-MkKxNlI2PjN:L3G+
U2OS	MkPQSpVv[3Srb36gZZN{[Xl?		NUGwSVdKOjRiaILz	NYDrZW13SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHPv[ZhxemW\|c3nu[{BxS02[LXjTVmM{KGG\|c3Xzd4VlKGG\|IHz1Z4ln\XKjc3WgZYN1cX[rdImgZYZ1\XJiMkSgbJJ{KGK7IILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFAyPc7:TR?=	NV:0cmpCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ\|PD;hQi=>
HEC-1	NHvRbnVHfW6ldHnvckBie3OjeR?=		NIq5VZMzPCCqcoO=	MY\B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFXSZoV1[SCneIDy[ZN{\WRiaX6gTGVENTFiY3XscJMh[2:neIDy[ZN{cW6pIHLleIEu\2GuIHHzd4V{e2WmIHHzJIx2[2moZYLhd4Uh[WO2aY\peJkh[W[2ZYKgNlQhcHK\|IHL5JJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODB{zszN	NWH1e41MRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNlI2PjNpPkKyNVIzPTZ\|PD;hQi=>
U2OS	M1;vOGZ2dmO2aX;uJIF{e2G7		M4HmdlI1KGi{cx?=	M4TOVGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJj\XSjIHX4dJJme3OnZDDpckBWOk:VIHPlcIx{KGOxZYjwdoV{e2mwZzDwR21ZNWiVUlOzJIF{e2W\|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMEC0{txO	MoGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzMkK1OlMoRjJ{MUKyOVY{RC:jPh?=
BL21	M4[2T2Z2dmO2aX;uJIF{e2G7		MXmxJIhz	M1PYNWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKE6USVStV3JEOyCxZjDFVoFteGijIHzp[4Fv\CCkaX7kbY5oKGSxbXHpckBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJINmdGy\|IHHmeIVzKDFiaIKgZpkhXFJvRmLFWEBie3OjeTygSWM2OD1yLkCwNFU3\|ryP	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF{MkW2N{c,OjJzMkK1OlM9N2F-
BL21	M{\aPGZ2dmO2aX;uJIF{e2G7		NFXXd2YyKGi{	Mlv5RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDOMWhqezZvdHHn[4VlKHSncnLpeY0udGGkZXzs[YQhVlKLRD3TVmM{KG:oIFXSZoV1[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6g[ZhxemW\|c3XkJIlvKEW\|Y3jldolkcGmjIHPvcIkhSkx{MTDj[YxteyCjZoTldkAyKGi{IHL5JHRTNU[URWSgZZN{[XluIFXDOVA:OC5yMEC5{txO	M1TtXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
BL21	NUHLVlh7TnWwY4Tpc44h[XO\|YYm=		MWqxJIhz	MWDB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIF6tTIl{Pi22YXfn[YQhfGW{Ynn1cU1t[WKnbHzl[EBGWmKndHGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFLMNlEh[2WubIOgZZN{\XO\|ZXSgZZMhemWlcoXpeI1mdnRib3[g[ox2d3Knc3PlbY4udGGkZXzs[YQhW1KFMzDh[pRmeiBzIHjyJIJ6KFSULV\SSXQh[XO\|YYmsJGVEPTB;MD6wNFE2\|ryP	M33x[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
BL21	M1f6XGZ2dmO2aX;uJIF{e2G7		NIjsTpIyKGi{	M17iN2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUk1JcXN4LYTh[4dm\CC2ZYLibZVuNWyjYnXscIVlKEWUYXzwbIEhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGJNOjFiY3XscJMh[XO\|ZYPz[YQh[XNicnXjdpVqfG2nboSgc4Yh\my3b4Lld4NmcW5vbHHi[Yxt\WRiU2LDN{Bi\nSncjCxJIhzKGK7IGTSMWZTTVRiYYPzZZktKEWFNUC9NE4xODQQvF2=	M1qx[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUKyOVY{Lz5{MkGyNlU3OzxxYU6=
Ishikawa cells	M1TVdGZ2dmO2aX;uJIF{e2G7		NYDObI1KOyCmYYnz	MorxRYdwdmm\|dDDhZ5Rqfmm2eTDheEBme3S{b3flckBz\WOncITvdkBqdiCqdX3hckBKe2ira3H3ZUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBw\iCjbHvhcIlv\SCyaH;zdIhifGG\|ZTDs[ZZmdCCjZoTldkA{KGSjeYOgeZNqdmdiUD3ubZRzd3CqZX75cEBxcG:\|cHjheIUh[XNic4Xid5Rz[XSnLDDFR\|UxRTBwMECwPe69VQ>?	M1joU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUe4OVUzLz5{MkG3PFU2OjxxYU6=
HepG2	NXOwdpJUTnWwY4Tpc44h[XO\|YYm=		NX:yPXE6OjRiaILz	M2fkbmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDlfJBz\XO\|ZXSgbY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OD1yLkCwNlLPxE1?	M{jnd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN\|I5Lz5{MkK4N\|MzQDxxYU6=
HepG2	NHXMUnhHfW6ldHnvckBie3OjeR?=		NXLUUVRtOjRiaILz	Ml36RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUJOwG0>	M3G4WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkizN\|I5Lz5{MkK4N\|MzQDxxYU6=
MCF7	NUnmOY4xSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M3LxPFczKGi{cx?=	M4LRN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6t[IVncWOrZX70JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1yLkCwNFAxPjUQvF2=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4NE[4NUc,OjJ2NkS2PFE9N2F-
MCF7:D5L	MWTGeY5kfGmxbjDhd5NigQ>?		M4\zZ\|E3KGi{cx?=	M13DfmV{fHKxZ3XubYMh[WO2aY\peJkh[XRiZYP0do9o\W5icnXj[ZB1d3JiaX6gbJVu[W5iTVPGO\|pFPUxiY3XscJMh[29vZYjwdoV{e2mwZzDFVmUh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIx2[2moZYLhd4Uh\2WwZTDlfJBz\XO\|aX;uJIFnfGW{IEG2JIhzeyCkeTDtbYNzd3CuYYTlJJJm[WSncjygSWM2OD1yLkCwNFAyQTcQvF2=	M2PNO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[0OlgyLz5{MkS2OFY5OTxxYU6=
Ishikawa cells	M2\ZbGZ2dmO2aX;uJIF{e2G7		MVK3NkBpenN?	NXTxUoVzTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDld5Rzd2enbjDy[YNmeHSxcjDpckBpfW2jbjDJd4hqc2G5YTDj[YxteyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WytYXzpcoUheGixc4DoZZRie2ViZYjwdoV{e2mxbjDh[pRmeiB5MjDodpMh[nlibXnjdo9xdGG2ZTDy[YFl\XJuIFXDOVA:OC5yMECwNlQ3\|ryP	NYjhbldDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
Ishikawa cells	NX\sfFFQTnWwY4Tpc44h[XO\|YYm=	MlTpNE4yKG6P	NE\E[ZA4OiCqcoO=	NFPkdmJG\m[nY4Sgc44h[2WubDDwdo9tcW[ncnH0bY9vKG:oIHj1cYFvKEm\|aHnrZZdiKGOnbHzzJIF1KDBwMTDuUUBi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5	NYPHZVFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0OlQ3QDFpPkKyOFY1PjhzPD;hQi=>
CHO-K1	MV\GeY5kfGmxbjDhd5NigQ>?		Mn\6NlQhcHK\|	NHrP[opVemGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBw\iCHUnLleIEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMEO2{txO	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
CHO-K1	MYXGeY5kfGmxbjDhd5NigQ>?		MUKyOEBpenN?	NYrS[FFLXHKjboPjdolxfGmxbnHsJIFkfGm4YYTpc44hd2ZiRWLhcJBp[SC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwOe69VQ>?	M3TrR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkS3NlE4Lz5{Mk[0O\|IyPzxxYU6=
yeast AH109	MknaSpVv[3Srb36gZZN{[Xl?		NU[5WW9oOjRiaILz	NUfxd4xbSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKHKnY3XweI9zKEyERDDlfJBz\XO\|ZXSgbY4hgWWjc4SgRWgyODliY3XscJMh[XO\|ZYPz[YQh[XNiY3;tdI92dmRiY3;uZ4VvfHKjdHnvckBifCC5aHnjbEAyOCViYXfvcol{fCCjY4Tpeol1gSCxZjD0bIUhdWG6aX31cUBi[3Srdnn0fUBw\iCHMjDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG\|ZTDhd5NigSxiQXP0bZZqfHl;MD6wNFAy\|ryP	NULETlA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
yeast AH109	MV;GeY5kfGmxbjDhd5NigQ>?		NWTLUIVvOjRiaILz	NEnpfm5C\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W\|c3XkJIF{KGOxbYDveY5lKGOxbnPlcpRz[XSrb36gZZQhf2irY3igNVAmKGGpb37pd5Qh[WO2aY\peJkhd2ZidHjlJI1igGmvdX2gZYN1cX[rdImgc4YhTTJiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEGldHn2bZR6RTBwMECwNVjPxE1?	MlPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
yeast AH109	NGXpV5FHfW6ldHnvckBie3OjeR?=		MkLMNlQhcHK\|	MXnB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGgdoVk\XC2b4KgUGJFKGW6cILld5Nm\CCrbjD5[YF{fCCDSEGwPUBk\WyuczDhd5Nme3OnZDDhd{BqdnSncnHjeIlwdiC5aYToJHNTSzFiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEWFNUC9NE4xODFyNd88US=>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
yeast AH109	NEjnVlRHfW6ldHnvckBie3OjeR?=		MXSyOEBpenN?	MVXB[49vcXO2IHHjeIl3cXS7IHH0JGVT[mW2YTDy[YNmeHSxcjDMRmQh\XiycnXzd4VlKGmwIInlZZN1KEGKMUC5JINmdGy\|IHHzd4V{e2WmIHHzJIlvfGW{YXP0bY9vKHerdHigV3JEOSCjZoTldkAzPCCqcoOgZpkh[WyyaHGt[4Ft[WO2b4Pp[IF{\SCjc4PhfUwhTUN3ME2wMlAxOTB6zszN	NGrMTog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O\|IyPyd-MkK2OFczOTd:L3G+
U2-OS	MnXtSpVv[3Srb36gZZN{[Xl?			NXjUO3JtSWO2aY\heIlwdiCxZjDoeY1idiCHUnHsdIhiKGW6cILld5Nm\CCrbjDoeY1idiCXMj3PV{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XlicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGVEPTB;MD6wNFAxODUQvF2=	NV3kcWNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOFMzPDJpPkKzNFQ{OjR{PD;hQi=>
U2-OS	MlHrSpVv[3Srb36gZZN{[Xl?			M{PQfGFkfGm4YYTpc44hd2ZiaIXtZY4hTVKkZYThJIV5eHKnc4Pl[EBqdiCqdX3hckBWOi2RUzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZkhemWuYYTpeoUhfG9idX70doVifGWmIHPvcpRzd2xuIFXDOVA:OC5yMECwNe69VQ>?	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
MCF7/2a	NUXNOXFDTnWwY4Tpc44h[XO\|YYm=			M2Xld2FkfGm4YYTpc44hd2ZiRWLhcJBp[SCrbjDoeY1idiCPQ1[3M\|JiKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTDy[YxifGm4ZTD0c{B2dnS{ZXH0[YQh[2:wdILvcEwhTUN3ME2wMlAxODIQvF2=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB2M{K0Nkc,OjNyNEOyOFI9N2F-
MCF7	MY\GeY5kfGmxbjDhd5NigQ>?	NEfwWVYyKHWP		NHHtXWpFd3ewIILl[5Vt[XSrb36gc4YhTVKjbIDoZUBpfW2jbjDNR2Y4KGOnbHzzJIF1KDFidV2gZpkhX2W\|dHXyckBjdG:2dHnu[y=>	NFrwVIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C0N\|I1Oid-MkOwOFMzPDJ:L3G+
HeLa	NYr5U3BvTnWwY4Tpc44h[XO\|YYm=	NXvIOoVpOSCwTR?=	MYeyNEB1dyB{NDDodpM>	NEjVeGlC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWUYXzwbIEu[mG\|ZXSgeJJidnOlcnnweIlwdiCjdDCxJI5OKGGodHXyJFIxKHSxIEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2OEO0Okc,OjN2NEizOFY9N2F-
MCF7	Mn\hSpVv[3Srb36gZZN{[Xl?	M2HxPFEhdk1?	NGW5c4IzOCC2bzCyOEBpenN?	M3fJTmFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBz\WOncITvdkBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHHjeIl3[XSrb36gc4YhTVKjbIDoZU1j[XOnZDD0doFve2O{aYD0bY9vKGG2IEGgcm0h[W[2ZYKgNlAhfG9iMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	NFO0ZVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0PFM1Pid-MkO0OFg{PDZ:L3G+
MCF7:WS8	Ml;MSpVv[3Srb36gZZN{[Xl?		MnOxOFghcHK\|	NFXRc2pC\2:waYP0JIFkfGm4aYT5JIF1KEWUIHnuJIh2dWGwIF3DSlc7X1N6IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJJBUOiCpZX7lJIV5eHKnc4Ppc44h[XRiMUCnMVExKE1iYX\0[ZIhPDhiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=>	MmLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEWxPVkoRjJ2OEC1NVk6RC:jPh?=
MCF7:WS8	MX;GeY5kfGmxbjDhd5NigQ>?		M3Lwc\|Q5KGi{cx?=	M4DBSGFod26rc4SgZYN1cX[rdImgZZQhTVJiaX6gbJVu[W5iTVPGO\|pYWzhiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gS3JGSjFiZ3Xu[UBmgHC{ZYPzbY9vKGG2IEGwK{0yOCCPIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM>	MkmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEWxPVkoRjJ2OEC1NVk6RC:jPh?=
MCF7:WS8	NYHhTZF3TnWwY4Tpc44h[XO\|YYm=		NHTNWIE1QCCqcoO=	NHGyXlhC\2:waYP0JIFkfGm4aYT5JIF1KEWUIHnuJIh2dWGwIF3DSlc7X1N6IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJHBoWiCpZX7lJIV5eHKnc4Ppc44h[XRiMUCnMVExKE1iYX\0[ZIhPDhiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=>	M3zsU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEC1NVk6Lz5{NEiwOVE6QTxxYU6=
MCF7	Mm[zRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NYPkdFBPOiCmYYnz	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO\|YYmsJGlEPTB;MkeuN\|XPxE1?	NWO1UGRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNlI5PzZpPkK1NlIzQDd4PD;hQi=>
MDA-MB-231	MnXFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NW[2No9UOiCmYYnz	MoW1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbJVu[W5iRWKtcoVo[XSrdnWgUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB{IHThfZMh[nliTWTUJIF{e2G7LDDJR\|UxRTN\|LkS2{txO	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{Mki3Okc,OjV{MkK4O\|Y9N2F-
B16F10	NYHFPZltSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M1XXelIh\GG7cx?=	MmHzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgcY92e2ViQkG2SlExKGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO\|YYmsJGlEPTB;M{muPFHPxE1?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{Mki3Okc,OjV{MkK4O\|Y9N2F-
MIAPaCa2	MXfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MWKyJIRigXN?	M3m2SGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIh2dWGwIF3JRXBiS2F{IHPlcIx{KGGodHXyJFIh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9OFIvQTQQvF2=	NVPsO2RmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyNlI5PzZpPkK1NlIzQDd4PD;hQi=>
HEK293	MmLQSpVv[3Srb36gZZN{[Xl?			NGfOemxC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwNlTPxE1?	MkftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|MEW2PFgoRjJ3M{C1Olg5RC:jPh?=
HEK293	M{ntfWZ2dmO2aX;uJIF{e2G7			MnTtRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDFVoJmfGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODByOEZOwG0>	M2i5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C1Olg5Lz5{NUOwOVY5QDxxYU6=
MCF7	MoriSpVv[3Srb36gZZN{[Xl?		MnSwNlQhcHK\|	NVLybVlKSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmGucHjhJIlvKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNicILv[4V{fGW{b37lJJJm[2WydH;yJIVv\G:pZX7veZMh\2WwZYOgZYN1cX[jdHnvckBi\nSncjCyOEBpenNiYomgdXBEWixiRVO1NF0xNjByMECwPFTPxE1?	MkPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NUmyNVMoRjJ3NUW5NlE{RC:jPh?=
HEC1	NGLMcZlHfW6ldHnvckBie3OjeR?=		Mmr2NlQhcHK\|	M{DSTWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJidHCqYTDlfJBz\XO\|ZXSgbY4hcHWvYX6gTGVEOSClZXzsd{Bie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjZoTldkAzPCCqcoOgZpkhTVKHLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SC2cnHud4Zm[3Srb36gZZN{[XluIFXDOVA:OC5yMECxOu69VQ>?	NUHO[pZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVkzOTNpPkK1OVU6OjF\|PD;hQi=>
MCF7	NHPSbmlHfW6ldHnvckBie3OjeR?=		MoO4NlQhcHK\|	NUPJWYVwSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBGWmKndHGgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCxdIXiZYlvKDJiZX7kc4dmdm:3czDn[Y5meyCjY4TpeoF1cW:wIHHmeIVzKDJ2IHjyd{BjgSCzUFPSMEBGSzVyPUCuNFAxO87:TR?=	NFizOJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1PVIyOyd-MkW1OVkzOTN:L3G+
HEC1	M1H5RWZ2dmO2aX;uJIF{e2G7		NXLZ[WRKOjRiaILz	M4jteWFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gSXJj\XSjIHX4dJJme3OnZDDpckBpfW2jbjDISWMyKGOnbHzzJIF{e2W\|c3XkJIF{KHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGGodHXyJFI1KGi{czDifUBGWkVvbIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKHS{YX7z[oVkfGmxbjDhd5NigSxiRVO1NF0xNjByMEO4{txO	NW\ze3Z6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVkzOTNpPkK1OVU6OjF\|PD;hQi=>
MCF7	M{D2RmZ2dmO2aX;uJIF{e2G7	NUfqe\|JlOTBibl2=	NXXJVpJmOjBiaILz	NW\sTHVCUW6mdXP0bY9vKG:oIHXzeJJw\2WwIILlZ4VxfG:{LXHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgZZQhOTBibl2gZYZ1\XJiMkCgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	MnvwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{m0PFUoRjJ3OEe5OFg2RC:jPh?=
Saos2	NYm5Rng3TnWwY4Tpc44h[XO\|YYm=	M2mxfVEhfU1?	NUPNTXM3PyCmYYnz	MXXB[49vcXO2IHHjeIl3cXS7IHH0JGVT[WyyaHGgbY4hcHWvYX6gV4FwezJiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCBzIIXNJIFnfGW{IEeg[IF6eyCkeTDNWHMh[XO\|YYm=	M2m2fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUW1OFcxLz5{N{G1OVQ4ODxxYU6=
Saos2	MlXGSpVv[3Srb36gZZN{[Xl?	M2XRSVAvOSCwTR?=	NWKwV\|hGOyCmYYnz	MkHVRYdwdmm\|dDDhZ5Rqfmm2eTDheEBGWmGucHjhJIlvKGi3bXHuJHNid3N{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJGFNWCCjY4Tpeol1gSC3c3nu[{BxNW6rdILvdIhmdnmuIIDoc5NxcGG2ZTDhd{B{fWK\|dILheIUh[XRiMD6xJI5OKGGodHXyJFMh\GG7czDifUBkd2yxcnnt[ZRzcWNiYYPzZZk>	Ml7XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW0O\|AoRjJ5MUW1OFcxRC:jPh?=
insect cells	NIPCSoxHfW6ldHnvckBie3OjeR?=			M16wfmRqe3CuYXPlcYVvfCCxZjDGcJVwem2xbnWgSXMzKEe{ZXXuJIZzd21iaIXtZY4hemWlb33ibY5idnRiZoXscEBt\W6pdHigSXJidHCqYTDlfJBz\XO\|ZXSgbY4hcW6\|ZXP0JINmdGy\|IH3lZZN2emWmIIXwJJRwKDRiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[XO\|YYmsJGlEPTB;MD6wNFQ1\|ryP	M{nM[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N\|c2Lz5{N{[0O\|M4PTxxYU6=
insect cells	MXnGeY5kfGmxbjDhd5NigQ>?		NVrVVGVXOiCqcoO=	MmCzSIl{eGyjY3Xt[Y51KG:oIH\seY9zdW:wZT3sZYJmdGWmIFXTNkBnem:vIILlZ49u[mmwYX70JIZ2dGxibHXu[5RpKGi3bXHuJGVT[WyyaHGgcIlo[W6mIHLpcoRqdmdiZH;tZYlvKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMh[W[2ZYKgNkBpenNiYomgWHIuTlKHVDDhd5NigSxiSVO1NF0xNjByMESx{txO	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDFyNUK4N{c,OjhzMEWyPFM9N2F-
insect cells	NEfI[IxHfW6ldHnvckBie3OjeR?=			MoD4TY5pcWKrdHnvckBw\iCobIXvdo1wdmViRWOyJIJqdmSrbnegeI8hemWlb33ibY5idnRiZoXscEBt\W6pdHigbJVu[W5iRWLi[ZRiKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCjc4PhfUwhUUN3ME2wMlAxPjZ3zszN	Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5M{WyNVQoRjJ6N{O1NlE1RC:jPh?=
Ishikawa cells	M2rDSmZ2dmO2aX;uJIF{e2G7		MXu5OkBpenN?	MlHVSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZUBqdiCqdX3hckBKe2ira3H3ZUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiYXzrZYxqdmVicHjvd5Bp[XSjc3WgZYN1cX[rdImgeZNqdmdicD3ObZRzd3CqZX7vcEBxcG:\|cHjheIUh[XNic4Xid5Rz[XSnIIDy[ZRz\WG2ZXSg[o9zKDl4IHjyd{Bnd2yub4fl[EBjgSC\|dXLzeJJifGViYXTkbZRqd25ibXXhd5Vz\WRiZY\ldpkhOTVic3Xjd{BjgSC\|Y3HucolvNCCHQ{WwQVAvODByMt88US=>	M1fCOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEGyPFkzLz5{OEixNlg6OjxxYU6=
293 cells	NFe3TGdHfW6ldHnvckBie3OjeR?=			NHTsVW1DcW6maX7nJIFn\mmwaYT5JJRwKEWUIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJHVCWy2kbHGgS5JqeFSrdHWgNlk{KGOnbHzzJIJ6KFOnbHXjeEB{[3KnZX6gZ49ueGW2aYTpeoUh[mmwZHnu[{Bie3OjeTygSWM2OD1yLkCwNlPPxE1?	M3HVZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEi2PVk3Lz5{OEi4Olk6PjxxYU6=
HEk293	MYrGeY5kfGmxbjDhd5NigQ>?			M2fnWWFod26rc4SgZYN1cX[rdImgZZQhemWlb33ibY5idnRiaIXtZY4hT0GOND3meZNm\CCHUnHsdIhiKGW6cILld5Nm\CCrbjDISYszQTNiY3XscJMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyPUCuNFAxOTIQvF2=	M3LZWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK1N\|M2Lz5{OUWyOVM{PTxxYU6=
Ishikawa	MX;GeY5kfGmxbjDhd5NigQ>?		MXO5OkBpenN?	NYm5fVVDTXO2cn;n[Y5q[yCjY4Tpeol1gSCjdDDFVoFteGijIHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSC3c3nu[{BxNW6rdILvdIhmdm:uIHHzJJN2[nO2cnH0[UB1emWjdHXkJIZweiB7NjDodpMh\m:ubH;3[YQh[nlic4Xid5Rz[XSnIHHk[Il1cW:wIHL5JJNx\WO2cn;wbI91d22ndILpZ{Bu\XSqb3SsJGVEPTB;MD6wNFAxO87:TR?=	NFXaXWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[0NVIxPid-Mkm2OFEzODZ:L3G+
MDA-MB-231/beta41	NYKzNnZETnWwY4Tpc44h[XO\|YYm=		MmDrNVghcHK\|	Ml3qSZN1em:pZX7pZ{Bi[3Srdnn0fUBifCCHUnLleIEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hVUSDLV3CMVI{OS:kZYThOFEh[2WubIOgZYZ1\XJiMUigbJJ{KGK7IILlcoltdGFibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBGSzVyPUCuNFAxODQQvF2=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ2MUKwOkc,Ojl4NEGyNFY9N2F-
insect cells	NYTINGJlTnWwY4Tpc44h[XO\|YYm=		NIfyTpkzKGi{cx?=	MYHJcohq[mm2aX;uJI9nKG[udX;yc41wdmViYnnu[Ilv\yC2bzDy[YNwdWKrbnHueEBpfW2jbjDmeYxtKGynbnf0bEB2dnSjZ3fl[EBGWmKndHGg[ZhxemW\|c3XkJIlvKGmwc3XjeEBk\WyuczDh[pRmeiB{IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5MEBKSzVyPUCuNFAyOjkQvF2=	M3r1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OE[5OVA{Lz5{OUi2PVUxOzxxYU6=
insect cells	NHnhS4RHfW6ldHnvckBie3OjeR?=		MVmyJIhzew>?	MofkTY5pcWKrdHnvckBw\iCobIXvdo9ud26nIHLpcoRqdmdidH:gdoVkd22kaX7hcpQhcHWvYX6g[pVtdCCuZX7neIghfW62YXfn[YQhTVKjbIDoZUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJIFnfGW{IEKgbJJ{KGK7IH\seY9z\XOlZX7j[UBxd2yjcnn6ZZRqd25iYYPzZZktKEmFNUC9NE4xODR6MEVOwG0>	M2PhS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OE[5OVA{Lz5{OUi2PVUxOzxxYU6=
insect cells	NFH5bnpHfW6ldHnvckBie3OjeR?=	NH\xUGMxNjFidH:gNVAxODBidV2=		MVvCbY5lcW6pIHHm[olvcXS7IITvJJVvfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JIZ2dGxibHXu[5RpKEWUYXzwbIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{AxNjFidH:gNVAxODBidV2gZpkhTmy3b4Ltc45mKEWVMjDHdoVmdiCmaYPwcIFk\W2nboSgZoF{\WRiYYPzZZk>	NHjufVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEC5NVkzOCd-M{CwPVE6OjB:L3G+
293 cells	NVzBPWh3TnWwY4Tpc44h[XO\|YYm=			Moj0RYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUx2IFTORU1jcW6maX7nJIRwdWGrbjDmeZNm\CCHUnHsdIhiKCi3bnvuc5dvKG:{aXfpckkhdGmpYX7kJIJqdmSrbneg[I9u[WmwIHX4dJJme3OnZDDpckBWSVNvYnzhJGdzcXCWaYTlJFI6OyClZXzsd{Bie3Onc4Pl[EBieyCkZYThMYxi[3SjbXHz[UB1emGwc3PybZB1cW:wYXygZYN1cX[jdHnvckBjgSCIUlXUMYJie2WmIFflcoVDVEG8ZYKgZZN{[XluIFnDOVA:OC5yMECxNFfPxE1?	NGHLZ\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEG0OFY6Pyd-M{CxOFQ3QTd:L3G+
293 cells	M4LxPGZ2dmO2aX;uJIF{e2G7			MYrB[49vcXO2IHHjeIl3cXS7IHH0JGdCVDRiRF7BMYJqdmSrbneg[I9u[WmwIH\1d4VlKEWUYnX0ZUApfW6tbn;3ckBwemmpaX6pJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gWWFUNWKuYTDHdolxXGm2ZTCyPVMh[2WubIOgZZN{\XO\|ZXSgZZMh[mW2YT3sZYN1[W2jc3WgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZpkhTlKHVD3iZZNm\CCJZX7lRmxCgmW{IHHzd4F6NCCLQ{WwQVAvODByNUe5{txO	MoDuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzNES2PVcoRjNyMUS0Olk4RC:jPh?=
HEK293	M1vtVWZ2dmO2aX;uJIF{e2G7			MXXT[Yxm[3SrdnWg[ZN1em:pZX6gdoVk\XC2b4Kg[I94di2{ZXf1cIF1d3JiYXP0bZZqfHliYYSgSmxCTy22YXfn[YQhTVKjbIDoZUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIFXSZYxxcGFiZHXndoFl[XSrb36gZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR\|UxRTBwMECwNFnPxE1?	M13EUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOUSwOVY2Lz5\|MEm0NFU3PTxxYU6=
HEK293	MkXNSpVv[3Srb36gZZN{[Xl?			MWDB[49vcXO2IHHjeIl3cXS7IHH0JGZNSUdvdHHn[4VlKEWUYXzwbIEhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDFVk1idHCqYT3t[YRq[XSnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODByMUROwG0>	M2q5eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOUSwOVY2Lz5\|MEm0NFU3PTxxYU6=
Sf9	NFvFZ5BHfW6ldHnvckBie3OjeR?=			NHH5c2tDcW6maX7nJGF{e2G7OjDUbIUhdWW2aH;kJIVueGyxeXXkJJdieyCjZHHweIVlKG[{b32geIhmKHOlaXXueIlncWNibHn0[ZJifHW{ZTDhcoQh\GW\|Y4LpZoVlKGmwIHTleIFqdCCkeTDPd4JwfXKwIHX0JIFtNiBqMUm5N{whSmmxY3jlcYl{fHK7LDCzNkwhPjJ{OT22NlM3MS5iUnXjc41jcW6jboSgbJVu[W5iRWLhcJBp[SCjbnSgSXJTKHC{b4TlbY5{KHencnWgdJVzcW[rZXSg[pJwdSC2cnHud4Zm[3SnZDDT[lku[2WubIOuJHRp\SCrbjD2bZRzdyBuIFvpQVAvODByMEG1{txO	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN\|UwLz6FaFXNRmw9N2F-
Sf9	MYXGeY5kfGmxbjDhd5NigQ>?			M4HQVGlvcGmkaYTpc44hSXO\|YYm6JHRp\SCvZYToc4Qh\W2ybH;5[YQhf2G\|IHHkZZB1\WRiZoLvcUB1cGVic3Pp[Y51cW[rYzDsbZRmemG2dYLlJIFv\CCmZYPjdolj\WRiaX6g[IV1[WmuIHL5JG9{[m:3bTDleEBidC5iKEG5PVMtKEKrb3Po[Y1qe3S{eTygN\|ItKDZ{MkmtOlI{PilwIGLlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiYX7kJGVT[mW2YTDwdo91\WmwczD3[ZJmKHC3cnnmbYVlKG[{b32geJJidnOoZXP0[YQhW2Z7LXPlcIx{NiCWaHWgbY4hfixiS3m9NE4xODByMUZOwG0>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN\|UwLz6FaFXNRmw9N2F-
HEK293T	NYe1TYZDTnWwY4Tpc44h[XO\|YYm=		MlTQNlQhcHK\|	Ml[wRYdwdmm\|dDDhZ5Rqfmm2eTDheEBGWi2kZYThJEh2dmuwb4fuJI9zcWerbjmgeJJidnOoZXP0[YQhcW5iSFXLNlk{XCClZXzsd{Bi\nSncjCyOEBpenNiYomgV5Rm[WS7LVfsc{BtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMECwN\|k5\|ryP	NH:zOGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
HEK293T	M1HZUGZ2dmO2aX;uJIF{e2G7		M2mxSFI1KGi{cx?=	NFz0[IRC\2:waYP0JIFkfGm4aYT5JIF1KEWULXHsdIhiKCi3bnvuc5dvKG:{aXfpckkhfHKjboPm[YN1\WRiaX6gTGVMOjl\|VDDj[YxteyCjZoTldkAzPCCqcoOgZpkhW3SnYXT5MWdtdyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTB;MD6wNFAy\|ryP	NWDTdnR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN3Lze+R4hGVUKOPD;hQi=>
Sf9	MoqxSpVv[3Srb36gZZN{[Xl?			MkDLRolv\GmwZzDBd5NigTpiVHjlJI1mfGixZDDlcZBtd3mnZDD3ZZMh[WSjcITl[EBnem:vIITo[UB{[2mnboTp[olkKGyrdHXyZZR2emViYX7kJIRme2O{aXLl[EBqdiCmZYThbYwh[nliT4Pic5VzdiCndDDhcE4hMDF7OUOsJGJqd2OqZX3pd5RzgSxiM{KsJFYzOjlvNkKzOkkvKFKnY3;tZolv[W62IHj1cYFvKEWUYXzwbIEh[W6mIFXSVkBxem:2ZXnud{B4\XKnIID1dolncWWmIH\yc40hfHKjboPm[YN1\WRiU3[5MYNmdGy\|LjDUbIUhcW5idnn0do8hNCCNaU2wMlAxODJzzszN	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN\|UwLz6FaFXNRmw9N2F-
Sf9	M1rrWmZ2dmO2aX;uJIF{e2G7			MV3Jcohq[mm2aX;uJGF{e2G7OjDUbIUhdWW2aH;kJIVueGyxeXXkJJdieyCjZHHweIVlKG[{b32geIhmKHOlaXXueIlncWNibHn0[ZJifHW{ZTDhcoQh\GW\|Y4LpZoVlKGmwIHTleIFqdCCkeTDPd4JwfW1iZYSgZYwvKChzOUmzMEBDcW:laHXtbZN1enluIEOyMEA3OjJ7LU[yN\|YqNiCUZXPvcYJqdmGwdDDoeY1idiCHUnHsdIhiKGGwZDDFVoJmfGFicILveIVqdnNid3Xy[UBxfXKrZnnl[EBnem:vIITyZY5{\mWldHXkJHNnQS2lZXzsd{4hXGinIHnuJJYtKEurPUCuNFAxOjIQvF2=	MoHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
CV-1	MY\MeYNq\mW{YYPlJGF{e2G7			MlL2UJVkcW[ncnHz[UBCe3OjeUqgSZN1em:pZX6gdoVk\XC2b4KtcoVo[XSrdnWgR3YuOSCtaXTu[Zkh[2WubIOgZZJmKG2jaX70ZYlv\WRiaX6gSJVt[mWlY3:nd{Bud2SrZnnl[EBG[WeuZTfzJI1m\Gm3bTD3bZRpKDRwNTDnM2wh\2y3Y3;z[UB{fXCybHXt[Y51\WRid3n0bEAyOCViZnX0ZYwh[m:4aX7lJJNmenWvIHHu[EAyODBidX7peJMwdWxicHXubYNqdGyrbj3zeJJmeHSxbYnjbY4h[XRiM{hCtEBENiCrbjDhJIh2dWmmaX\p[YQhPSViQ1:yJIF1dSxiSVO1NF0xNjByMUhOwG0>	M{O3e\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{PS9pPlPoSW1DVDxxYU6=
CV-1	M1zTbmx2[2moZYLhd4UhSXO\|YYm=			MYDMeYNq\mW{YYPlJGF{e2G7OjDFd5Rzd2enbjDy[YNmeHSxcj3u[YdifGm4ZTDDWk0yKGurZH7lfUBk\WyuczDhdoUhdWGrboThbY5m\CCrbjDEeYxj\WOlbzfzJI1w\GmoaXXkJGVi\2ynJ4OgcYVlcXWvIIfpeIghPC53IHevUEBodHWlb4PlJJN2eHCuZX3lcpRm\CC5aYToJFExLSCoZYThcEBjd3[rbnWgd4VzfW1iYX7kJFExOCC3bnn0d{9udCCyZX7pZ4ltdGmwLYP0doVxfG:veXPpckBifCB\|N9MwJGMvKGmwIHGgbJVucWSrZnnl[EA2LSCFT{KgZZRuNCCLQ{WwQVAvODBzOd88US=>	NXK0dGZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTN3Lze+R4hGVUKOPD;hQi=>
COS7	NELsUmVHfW6ldHnvckBie3OjeR?=			M4jhUmNwdXCndHn0bZZmKGKrbnTpcoch[W[oaX7peJkhfG9iRz3wdo91\WmwIHPveZBt\WRiZYP0do9o\W5icnXj[ZB1d3JiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{BjgSCHMjDBcIV5[SB4M{Ogd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJIFv[Wy7c3nzMEBMcT1yLkCwNlfPxE1?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN\|UwLz6FaFXNRmw9N2F-
MDA-MB-231	NWXyPGtGT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	M1vzSlEheE1idH:gNUDPxE1?	NYTIRYZmPDhiaIK=	M4GyV2dzd3e2aDDpcohq[mm2aX;uJI9nKEixbX:gd4FxcWWwczCobJVu[W5rIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAoNTF{IITvJFExLy14IF2gZYZ1\XJiNEigbJIh[nliTWTUJIF{e2G7	NGOyWoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
UMR106	MWjGeY5kfGmxbjDhd5NigQ>?	NHH6T3AyOCCwTR?=	NIXvT40zPCCqcoO=	M1LNTXRz[W6\|YXP0bZZifGmxbjDv[kBpfW2jbjDFVk1idHCqYTD0doFve2[nY4Tl[EBqdiC{YYSgWW1TOTB4IHPlcIx{KGG2IEGwK{05KE1iYX\0[ZIhOjRiaILzJIJ6KGS3YXygcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6	M2exeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE{PS9pPlPoSW1DVDxxYU6=
UMR106	NV\oOJBQTnWwY4Tpc44h[XO\|YYm=	NGfIZm0yOCCwTR?=	Mn;GNlQhcHK\|	MVTUdoFve2GldHn2ZZRqd25ib3[gbJVu[W5iRWKtZoV1[SC2cnHud4Zm[3SnZDDpckBz[XRiVV3SNVA3KGOnbHzzJIF1KDFyJz24JG0h[W[2ZYKgNlQhcHK\|IHL5JIR2[WxibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	NFnPN\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOzVxJ{7DbGVOSkx:L3G+
U2OS	NFzYUIRHfW6ldHnvckBie3OjeR?=	NVXm[m94OC5yMTD1US=>	MnPoNlQhcHK\|	NE\XWHlC[3SrdnH0bY9vKG:oIFXSZoV1[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCXMl;TJINmdGy\|IHH0JFAvODFidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVM2Nyd-Q3jFUWJNRC:jPh?=
U2OS	NYDnOpRDTnWwY4Tpc44h[XO\|YYm=	M1;RWVAvODFidV2=	M{D0d\|I1KGi{cx?=	NGL0bmVC[3SrdnH0bY9vKG:oIFXSZYxxcGFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gbJVu[W5iVULPV{Bk\WyuczDheEAxNjBzIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxN\|UwLz6FaFXNRmw9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-GSK3β / GSK3 ; PubMed: 19360103 PLoS One (A)-Neuroblastoma N2a-m were treated with different doses of estradiol and for different times (20–120 min with 10–500 nM) and the maximum increase in GSK3β-PSer9 was observed when they were treated for 60 min with 100–200 nM estradiol. β-catenin; PubMed: 19360103 PLoS One (B)-Stabilization of β-catenin. N2a-m cells treated with estradiol showed a clear increase in β-catenin that could be prevented by prior exposure to ICI 182780 (used 100× concentrated in comparison to estradiol), 60 minutes before estradiol treatment (lower panel). Survivin / ER-alpha / NgBR ; PubMed: 24223763 PLoS One MCF-7 is an estrogen-dependent breast cancer cell line. NgBR was knocked down in MCF-7 cells using siRNA. (A) NgBR knockdown diminished estradiol-induced survivin expression in MCF-7 breast tumor cells. MCF-7 cells were treated with 10 nM estradiol for 6 and 48 hours. Protein levels of NgBR and survivin were determined by Western blot analysis. Beta-Actin is applied as a housekeeping protein. The density of each band was measured using NIH ImageJ and presented as relative intensity of survivin after normalized with beta-actin housekeeping protein. ERα / FOXP1 / KRAS / PTEN ; PubMed: 27441287 Heliyon Stimulation with different concentrations of estradiol. Levels of expression of ERα, FOXP1, and KRAS increased in accordance with the concentrations of estradiol.	19360103 24223763 27441287

In vivo

Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. ^[4] Estradiol alone can reverse the ovariectomy-induced decrease in spine density. Estradiol combined with Progesterone initially increases spine density for a period of 2 to 6 hours but then results in a much sharper decrease than is observed following estradiol alone. ^[5]

Protocol

References

- Collapse

Solubility (25°C)

In vitro	DMSO	54 mg/mL (198.25 mM)
	Water	Insoluble
	Ethanol	'5 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Estradiol SDF

Molecular Weight	272.38
Formula	C₁₈H₂₄O₂
CAS No.	50-28-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	17β-estradiol, β-Estradiol, E2, 17β-Oestradiol
Smiles	CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04189133	Not yet recruiting	Drug: Lutropin alfa\|Drug: Human chorionic gonadotropin	Acquired Hypogonadotropic Hypogonadism	Azienda Ospedaliero-Universitaria di Modena	October 1 2021	Phase 2
NCT05002738	Not yet recruiting	Drug: Desogestrel and Ethinyl Estradiol Tablets	Contraception	University of Colorado Denver	September 2021	Phase 4
NCT05021094	Not yet recruiting	Drug: Kuntai Capsule\|Drug: Femoston	Primary Ovarian Insufficiency	Affiliated Hospital of Nantong University	September 1 2021	Phase 4
NCT05003336	Not yet recruiting	Drug: Xiangshao Granules\|Drug: Xiangshao Granules Placebo	Anxiety\|Menopause	Peking Union Medical College Hospital	September 2021	Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Estrogen/progestogen Receptor Signaling Pathway Map

Related Estrogen/progestogen Receptor Products

Licochalcone A ^New

Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.
Endoxifen HCl ^New

Endoxifen HCl, the active metabolite of Tamoxifen, ia a potent and selective estrogen receptor antagonist. Phase 2.
Sacubitril/valsartan (LCZ696) ^New

Sacubitril/valsartan (LCZ696, Sacubitril, Valsartan), consisting of valsartan and sacubitril in 1:1 molar ratio, is an orally bioavailable, dual-acting angiotensin receptor-neprilysin inhibitor (ARNi) for hypertension and heart failure. Phase 3.
Fulvestrant (ICI-182780)

Fulvestrant (ICI-182780, ZD 9238, ZM 182780) is an estrogen receptor (ER) antagonist with IC50 of 0.94 nM in a cell-free assay. Fulvestrant also induces autophagy and apoptosis and has antitumor activity.
Tamoxifen (ICI 46474) Citrate

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.
Mifepristone (RU486)

Mifepristone (RU486, C-1073, RU 38486, Mifegyne) is a remarkably active antagonist of progesterone receptor and glucocorticoid receptor with IC50 of 0.2 nM and 2.6 nM, respectively. Mifepristone promotes cell autophagy and apoptosis, decreases Bcl-2 level and increases Beclin1 level, accompanied by weakened interaction between Bcl-2 and Beclin1.

Features:Mifepristone is the first approved medication for patients with endogenous cushing's syndrome.
Raloxifene HCl

Raloxifene (LY156758, Keoxifene) HCl a selective and orally active estrogen receptor modulator (SERM), which inhibits human cytosolic aldehyde oxidase-catalyzed phthalazine oxidation activity with IC50 of 5.7 nM.

Features:Raloxifene is as effective as tamoxifen in reducing the incidence of breast cancer in postmenopausal women at increased risk.
Toremifene Citrate (NK 622)

Toremifene Citrate (NK 622, NSC 613680) is an oral selective estrogen receptor modulator (SERM), used in the treatment of advanced breast cancer.
Progesterone (NSC 9704)

Progesterone (NSC 9704, NSC 64377, Pregn-4-ene-3,20-dione) is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.
Bazedoxifene (WAY-140424) HCl

Bazedoxifene HCl (WAY-140424, TSE-424) is a novel, non-steroidal, indole-based estrogen receptor modulator (SERM) binding to both ERα and ERβ with IC50 of 23 nM and 89 nM, respectively.

Choose Your Country or Region

By Signaling Pathways

By product type

Estradiol