Name: Allopurinol
Brand: Selleck Chemicals
SKU: S1630
Rating: 5 (19 reviews)

Allopurinol is a purine analog inhibitor of the enzyme xanthine oxidase, used to treat gout or kidney stones, and to decrease levels of uric acid.

Allopurinol Chemical Structure

CAS: 315-30-0

Selleck's Allopurinol has been cited by 19 publications

Purity & Quality Control

Batch: Purity: 99.98%

99.98

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Biological Activity

Description

Allopurinol is a purine analog inhibitor of the enzyme xanthine oxidase, used to treat gout or kidney stones, and to decrease levels of uric acid.

Targets

xanthine oxidase ^[1]

In vitro
In vitro	Allopurinol reverses the increased xanthine oxidase activity in ischemia-reperfusion injury of neonatal rat hearts. Allopurinol (10 mM) treatment suppresses xanthine oxidase activity induced by hypoxia-reoxygenation injury and the production of reactive oxygen species. Allopurinol also decreases the concentration of intracellular Ca2+ increased by enhanced xanthine oxidase activity. ^[1]

In Vivo
In vivo	Allopurinol shows abnormal pyrimidine metabolism together with renal toxicity which could be ameliorated by uridine, indicating that Allopurinol essentially causes pyrimidine metabolism abnormality leading to renal impairment in normal mice. Allopurinol increases urinary OD excretion to an extent similar to that in normal mice administered the same dose of Allopurinol in DNFB-sensitized mice. ^[2] Allopurinol promotes a clinical improvement which is accompanied by a reduction in the parasitic load in the blood, skin and lymph nodes but, even after long period of allopurinol administration alone, Leishmania may persist in dog tissues in Leishmania-infected dogs. ^[3] Allopurinol prevents early alcohol-induced liver injury in rats, most likely by preventing oxidant-dependent activation of NF-kappaB. ^[4] Allopurinol protects dose-dependently against acetaminophen-induced cell injury, the loss of ATP and the increase of the GSSG content in the total liver and in the mitochondrial compartment without inhibiting reactive metabolite formation in mice. Allopurinol almost completely inhibits hepatic xanthine oxidase and dehydrogenase activity, but only high doses prevents the increase of the mitochondrial GSSG content. ^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05665699	Recruiting	Gout	InventisBio Co. Ltd	April 17 2023	Phase 2
NCT05193838	Unknown status	Dilated Cardiomyopathy	Assiut University	May 1 2022	--
NCT05360628	Completed	Healthy	InventisBio Co. Ltd	November 1 2021	Early Phase 1
NCT04853615	Not yet recruiting	SGLT2i Kideny Protection Against Contrast in Diabetic Kidney	Fayoum University\|Cairo University	July 1 2021	--

References

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Chemical Information & Solubility

Molecular Weight	136.11	Formula	C₅H₄N₄O
CAS No.	315-30-0	SDF	Download Allopurinol SDF
Smiles	C1=NNC2=C1C(=O)NC=N2
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 27 mg/mL ( (198.36 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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