Home Endocrinology & Hormones Estrogen/progestogen Receptor modulator - CRISPR/Cas9 activator 4-Hydroxytamoxifen (Afimoxifene)

4-Hydroxytamoxifen (Afimoxifene)

Catalog No.S7827 Synonyms: Afimoxifene

For research use only.

4-Hydroxytamoxifen (Afimoxifene) is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants.

4-Hydroxytamoxifen (Afimoxifene) Chemical Structure

CAS No. 68392-35-8

Selleck's 4-Hydroxytamoxifen (Afimoxifene) has been cited by 13 publications

Purity & Quality Control

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Estrogen/progestogen Receptor Signaling Pathways

Estrogen/progestogen Receptor Signaling Pathway Map

Biological Activity

Description 4-Hydroxytamoxifen (Afimoxifene) is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants.
In vitro

4-OHT is a potent activator of ER and has been shown to have a higher affinity for estrogen receptors compared to tamoxifen. It has significant dose and time-dependent direct effects to alter the contractile function of isolated adult rat cardiac myocytes[1]. 4-HT activates on-target modification frequency for intein-Cas9 variants [3].
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells MVfQdo9tcW[ncnH0bY9vKGG|c3H5 MkK3TY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2|dHnteYxifGWmIF3DSk04KGOnbHygdJJwdGmoZYLheIlwdixiSVO1NF0xNjVibl2= MnHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTV2OEixO{c,QTV2OEixO|ww[T5?
MCF7 NYLsSmJzTnWwY4Tpc44h[XO|YYm= M4\vfWVn\mWldHn2[UBld3OnIH\vdkBcO0ifLTDld5Rz[WSrb3ygZYdicW6|dDDwdo9tcW[ncnH0bY9vKG:oIF3DSk04KGOnbHzzMEBGSzVyIE2gNE4xODByMzFOwG0v Mlq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OU[4NUc,Ojd4OU[4NVww[T5?
MCF7 MUfGeY5kfGmxbjDhd5NigQ>? MXq0JIhzew>? NWHYeZJWUW6mdXP0bY9vKG:oIFXSZYxxcGFiZHXndoFl[XSrb36gbY4hcHWvYX6gUWNHPyClZXzsd{BqdiCyaHXuc4whemWmIH\y[YUhWlCPSTDt[YRqfW1iY3;ueIFqdmmwZzC1KUBkcGG{Y3;hcEBl\Xi2cnHuMZRz\WG2ZXSgSmJUKGmwY4XiZZRm\CCob4KgOEBpenNiYomgV3AyKGGwZDDhcpRqNUWUIILhZoJqfCCvb37vZ49tdmGuIHHueIljd2S7IHLhd4VlKGmwLXPlcIwhX2W|dHXyckBie3OjeTygRYN1cX[rdImgQUAxNjByMEGg{txONg>? NHvuT3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC4OlYzPid-M{CwPFY3OjZ:L3G+
MCF7 M3PLUGZ2dmO2aX;uJIF{e2G7 MV\F[oZm[3SrdnWgZ49v[2WwdILheIlwdiCjZ3HpcpN1KE2FRj23JIJz\WG|dDD0eY1weiClZXzsd{B2e2mwZzDNR2YuPyCjc4PhfUwhTUN3MDC9JFAvODByMUKg{txONg>? NUjaRndJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK4NlU6OzVpPkGyPFI2QTN3PD;hQi=>
MCF7 NF34OFVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGLkc2VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTBiPTCwMlAxODVizszNMi=> NHnrNW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSwOVI5Pid-MkK0NFUzQDZ:L3G+
MCF7 NVfYbmJPSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NGXSWG9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTBiPTCwMlAxODV|NzFOwG0v MkTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MEewNVIoRjJ4NEC3NFEzRC:jPh?=
MCF MXfGeY5kfGmxbjDhd5NigQ>? Mn;NRY51[WexbnnzeEBi[3Srdnn0fUBifCCycn;n[ZN1\XKxbnWgdoVk\XC2b4KgbY4hcHWvYX6gUWNHKGOnbHzzJIF{e2W|c3XkJIF{KGW|dILh[IlwdC2rbnT1Z4VlKHKnY3XweI9zKHKnc4DvcpNmNCCLQ{WwJF0hOC5yMEC3PVQ{KM7:TT6= MoTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MEewNVIoRjJ4NEC3NFEzRC:jPh?=
Ishikawa endometrial cells NF\W[3pHfW6ldHnvckBie3OjeR?= NYnBZ|ZnSWexbnnzeEBi[3Srdnn0fUBieyCjbHvhcIlv\SCyaH;zdIhifGG|ZTDpcoR2[3Srb36gbY4hUXOqaXvhe4Eh\W6mb33leJJq[WxiY3XscJMh[2:vcHHy[YQhfG9iRUKsJGVEPTBiPTCwMlAxODhizszNMi=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN6MEKwPEc,OTV|OECyNFg9N2F-
MCF7 NI\sOGZHfW6ldHnvckBie3OjeR?= M3n4PGlvcGmkaYTpc44hd2ZiZYP0do9o\W5vaX7keYNm\CCycn;sbYZmemG2aX;uJIlvKGi3bXHuJG1ETi15IHLy[YF{fCClYX7j[ZIh[2WubIOsJGlEPTBiPTCwMlAxOTF5IN88UU4> NXfHNGNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUG1OFk3Oyd-OUG1OFk3OzxxYU6=
MCF7 MX3GeY5kfGmxbjDhd5NigQ>? NXLncW1iOjRiaILz NFvYTYNCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHXzeJJw\2WwLXnu[JVk\WRidILhcpNkemmydHnvckBxemWrbnP1ZoF1\WRib4\ldo5q\2i2IH\vcIxwf2WmIHL5JIV{fHKxZ3XuJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IEK0JIhzeyCkeTDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFA{KM7:TT6= NHTM[mM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK5OlM6QCd-MkiyPVY{QTh:L3G+
Rosetta 2 DE3 MXjGeY5kfGmxbjDhd5NigQ>? MUexJIhz NVzH[IY3TGm|cHzhZ4Vu\W62IH;mJHs{UF1vZYP0doFlcW:uIH\yc40hemWlb33ibY5idnRiaIXtZY4hVi22ZYLtbY5idCCKaYOteIFo\2WmIFXSZYxxcGFiTFLEJIhiemKxcnnu[{BEOzhzUz;DOFE4Wy:FNUOwV{BufXSjboSg[ZhxemW|c3XkJIlvKFKxc3X0eIEhOiCGRUOgZ49ueGW2ZX70JINmdGy|IHHmeIVzKDFiaIKgZpkhW1CDIHLpcoRqdmdiYYPzZZktKEmFNUCgQUAxNjByNDFOwG0v M1e2fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Mkm2N|k5Lz5{OEK5OlM6QDxxYU6=
insect cells NIXBdoVHfW6ldHnvckBie3OjeR?= NES1R4JKdmirYnn0bY9vKG:oIH\seY9zdW:wZTDFV|Ih\3KnZX6gZolv\GmwZzD0c{Bz\WOxbXLpcoFvfCCodXzsJIxmdme2aDDoeY1idiCHUnHsdIhiKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMh[nliZnz1c5Jme2OnbnPlJJBwdGG{aYrheIlwdiCjc4PhfUwhUUN3MDC9JFAvODB4Mkmg{txONg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd|NUKxOEc,Ojh5M{WyNVQ9N2F-
MCF-7-2a M3nicWZ2dmO2aX;uJIF{e2G7 NXHqblZNUW6qaXLpeIlwdiCxZjDld5Rz[WSrb3ygbY5lfWOnZDDld5Rzd2enbjDy[YNmeHSxcjD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDpckBOS0ZvNz2yZUBk\WyuczygTWM2OCB;IECuNFA4KM7:TT6= NWjLd2ZjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2O|IzPDlpPkGyOlczOjR7PD;hQi=>
MCF7 MkXsRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mk\lNlQhcHK| NULWTpJHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTBiPTCwMlAxQDVizszNMi=> NWn1N2dPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyO|IzPTZpPkG4NlczOjV4PD;hQi=>
MCF7 NFHqV5RHfW6ldHnvckBie3OjeR?= MYrJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCPQ1[tO{BjemWjc4SgeJVud3JiY3XscJMhfXOrbnegUWNHNTdiYYPzZZktKEmFNUCgQUAxNjByOEWg{txONg>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NUmzOUc,OTJ6MkW5N|U9N2F-
Ishikawa cells MoOzSpVv[3Srb36gZZN{[Xl? MlOyO|IhcHK| Moe4RY51[WexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZU9j\XSjIHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGGodHXyJFczKGi{czDifUBidGujbHnu[UBxcG:|cHjheIF{\SCjc4PhfUwhUUN3MDC9JFAvODFizszNMi=> NXn0eXNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NlY6OzFpPkK4OFI3QTNzPD;hQi=>
insect cells MmDlSpVv[3Srb36gZZN{[Xl? NEnmZllFcXOybHHj[Y1mdnRib3[g[ox2d3Knc3PlbY4hdGGkZXzl[EBme3S{YXTpc4wh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDFVoJmfGFiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBk\WyuczDifUBndHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7LDDJR|UxKD1iMD6wNkDPxE1w MkftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6M{WxO|YoRjF6OEO1NVc3RC:jPh?=
MCF7 MlXwR5l1d3SxeHnjbZR6KGG|c3H5 MXW1JIRigXN? M3jRZ2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOUBl[Xm|LDDJR|UxKD1iMD6wNlkyKM7:TT6= Mn7JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS5NlgoRjJ|OE[0PVI5RC:jPh?=
GT1-7 NHzofndHfW6ldHnvckBie3OjeR?= Mm\oNlAhfG9iMkSgbJJ{ NWPZU4EySW62YXfvcol{fCCjY4Tpeol1gSCjdDDld5Rzd2enbjDy[YNmeHSxcjDpckBud3W|ZTDHWFEuPyClZXzsd{Bp[XKkb4Lpcoch[mW2YT3nZYxi[3Sxc3nkZZNmKHKncH;yeIVzKGenbnWgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIEG3ZoV1[S2nc4TyZYRqd2xiaX7keYNm\CC{ZYPwc45{\SCvZXHzeZJm\CCjZoTldkAzOCC2bzCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yM{OyJO69VS5? M{XBSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NECwNlM6Lz5{OESwNFI{QTxxYU6=
insect cells M{nOW2Z2dmO2aX;uJIF{e2G7 MXfEbZNxdGGlZX3lcpQhd2ZiZnz1c5Jme2OnaX6gcIFj\WynZDDld5Rz[WSrb3yg[pJwdSCqdX3hckBz\WOxbXLpcoFvfCCHUnHsdIhiKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgZ4VtdHNiYomg[ox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigSxiSVO1NEA:KDBwMESg{txONg>? NE\6XIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVE4Pid-MUi4N|UyPzZ:L3G+
HEK293 NEO1SoxHfW6ldHnvckBie3OjeR?= NV7OTppJOzBibXnudy=> M3;jWGlvcGmkaYTpc44hd2ZiR1\QMZRi\2enZDDoeY1idiCSTFSyJIV5eHKnc4Pl[EBqdiCqdX3hckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4heGixc4DoZZRq\HmuYoX0ZY5wdC2dZEndJJBzd2S3Y4Tpc44h[W[2ZYKgN|AhdWmwczDifUBu[XO|IIPw[YN1em:vZYTybYMh[W6jbInzbZMtKEmFNUCgQUAxNjB2NDFOwG0v MnTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzM{[5O|UoRjF7MUO2PVc2RC:jPh?=
HEK293 NF\XWJdHfW6ldHnvckBie3OjeR?= MWKxJIhz NEfG[mpCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGW|dILh[IlwdC2rbnT1Z4VlKFmIUD3meZNm\CCVUlOxJINw[WO2aY\heI9zKHKnY4L1bZRu\W62IH3lZZN2emWmIHHmeIVzKDFiaIKgbY4heHKnc3XuZ4Uhd2ZiQ3;lcIVvfGW{YYrpcoUhUCCkeTDCVmVVKGG|c3H5MEBKSzVyIE2gNE4xPyEQvF2u M1vQclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEe4OlA6Lz5|MEC3PFYxQTxxYU6=
MCF7 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MUG3NkBpenN? MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{IHTldIVv\GWwdDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkGwO{DPxE1w MnuyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6M{WxO|YoRjF6OEO1NVc3RC:jPh?=
MCF7 NXG1OZFuSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4LlbFczKGi{cx?= NV:2SmdWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTD0dplx[W5iYnz1[UBie3OjeTygS2k2OCB;IECuNVA4KM7:TT6= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NUiyPUc,OjN5M{W4Nlk9N2F-
MCF7 M2HlW2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 M{i1e|czKGi{cx?= Mo\WRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFVk1l\XCnbnTlcpQhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNE4yOSEQvF2u NWDtfmpMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NlY6OzFpPkK4OFI3QTNzPD;hQi=>
T47D MY\DfZRwfG:6aXPpeJkh[XO|YYm= NYnyTJp4PSCmYYnz MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIFnfGW{IEWg[IF6eyxiSVO1NEA:KDBwMkGg{txONg>? MmPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS5NlgoRjJ|OE[0PVI5RC:jPh?=
Ishikawa endometrial cells NYj1fZo1TnWwY4Tpc44h[XO|YYm= MkLpRY51[WexbnnzeEBi[3Srdnn0fUBieyCrbnjpZol1cW:wIH;mJFEhdk1iMUetZoV1[S2nc4TyZYRqd2xic4TpcZVt[XSnZDDhcItidGmwZTDwbI9{eGijdHHz[UBqdmS3Y4Tpc44hcW5iSYPobYtif2FiZX7kc41mfHKrYXygZ4VtdHNuIFnDOVAhRSByLkWxJO69VS5? MnHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV|OECyNFgoRjF3M{iwNlA5RC:jPh?=
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Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot p-ERK / ERK / p-MEK / MEK1 / MEK2 / p21 / Cyclin D1 26163823
Growth inhibition assay Cell viability 26014809

Protocol (from reference)

Cell Research:

[1]
  • Cell lines: Adult Rat and Mouse Cardiac Myocytes
  • Concentrations: 0, 0.5, 1, 3, 5, and 10 µM
  • Incubation Time: 15-45 min
  • Method:

    For sarcomere length measurements, cells are covered in Tyrode’s containing 4OHT, allowed to reach 36 ± 1°C, and then stimulated at 0.2 Hz.
Animal Research:

[2]
  • Animal Models: Sprague-Dawley rats
  • Dosages: 21 mg/kg, 54 μmol/kg
  • Administration: oral administration

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 387.51
Formula

C26H29NO2
CAS No. 68392-35-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(=C(C1=CC=C(C=C1)O)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05133674 Not yet recruiting Drug: Tamoxifen 20 mg Breast Cancer|Breast Carcinoma|Breast Tumors|Cancer of Breast|Malignant Neoplasm of Breast Karolinska University Hospital January 10 2022 Phase 2
NCT03199963 Terminated Drug: 4-OH tamoxifen|Drug: Placebo Mammographic Breast Density BHR Pharma LLC August 21 2017 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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