Tubeimoside I

Catalog No.S3814 Synonyms: Lobatoside H

For research use only.

Tubeimoside I (Lobatoside H, TBMS1), a triterpenoid saponin, isolated from the tubers of Bolbostemma paniculatum, shows potent antitumor and antitumor-promoting effects.

Tubeimoside I Chemical Structure Tubeimoside I Chemical Structure

CAS No. 102040-03-9

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Biological Activity

Description Tubeimoside I (Lobatoside H, TBMS1), a triterpenoid saponin, isolated from the tubers of Bolbostemma paniculatum, shows potent antitumor and antitumor-promoting effects.
In vitro

TBMS I inhibits the proliferation of both HepG2 and L-02 cells in a dose- and time-dependent manner, but HepG2 cells appear more sensitive to the agent. When exposed to TBMS I for 24, 48 and 72 h, IC50 for HepG2 cells versus L-02 cells are 15.5 vs. 23.1, 11.7 vs. 16.2, 9.2 vs. 13.1 (μM, p<0.01), respectively. TBMS I induces cell shrinkage, nuclear condensation and fragmentation, cell cycle arrest at the G2/M phase, mitochondrial membrane disruption, release of cytochrome c from the mitochondria, activation of caspase 3 and 9, and shifting Bax/Bcl-2 ratio from being anti-apoptotic to pro-apoptotic, all indicative of initiation and progression of apoptosis involving mitochondrial dysfunction[1].

In vivo TBMS1 significantly inhibits the production of the pro-inflammatory cytokines, TNF-α, IL-6 and IL-1β in vitro and in vivo. Pretreatment with TBMS1 markedly attenuates the development of pulmonary edema, histological severities and inflammatory cells infiltration in mice with acute lung injury (ALI). TBMS1 exerts an anti-inflammatory effect in vivo model of ALI through suppression of IkB activation and p38/extracellular signal-regulated kinase mitogen-activated protein kinases signaling in a dose-dependent manner. TBMS1 could be a potent anti-tumor agent by inducing apoptosis in a variety of cancer types via the mitochondrial-related signaling pathway[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HepG2 cells, L-02 cells, HCCLM3 cells, or QSG-7701 cells
  • Concentrations: 5, 10, 15, 20, 30, 40, 90 μM
  • Incubation Time: 24, 48 and 72 h
  • Method: TBMS I cytotoxicity to cultured HepG2 cells, L-02 cells, HCCLM3 cells, or QSG-7701 cells are evaluated in terms of proliferation inhibition determined by the relative absorbance of MTT dye by the cells. In brief, cells (1×104 cells/well) are seeded in 96-well tissue culture plates and then cultured in RPMI-1640 growth medium for 24 h. Subsequently, the medium is replaced with RPMI 1640 growth medium containing designated concentrations of TBMS I (5, 10, 15, 20, 30, 40, 90 μM). Cells treated with sham containing equal volume of cell culture medium but no TBMS I (0 μM) are used as control in every experiment throughout this study. After exposure to TBMS I for 24, 48 and 72 h, MTT dye is added into each well at a final concentration of 0.5 mg/ml, and the plates are incubated for an additional 4 h at 37 °C. The insoluble formazan produced by the living cells in response to MTT dye is collected and dissolved in DMSO and measured with an ELISA reader at the wavelength of 492 nm. Each plate contained multiple wells for a given experimental condition and multiple wells of control. The procedure is replicated three times.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: BALB/c mice
  • Dosages: 1, 2 or 4 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(75.79 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1319.43
Formula

C63H98O29

CAS No. 102040-03-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1C(C(C(C(O1)OC2C(C(COC2OC(=O)C34CCC(CC3C5=CCC6C(C5(CC4)C)(CCC7C6(CC(C8C7(COC(=O)CC(CC(=O)OC9C(C(COC9OC1C(C(C(OC1O8)CO)O)O)O)O)(C)O)C)O)C)C)(C)C)O)O)O)OC1C(C(C(CO1)O)O)O)O

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