6-Gingerol

Catalog No.S3836

For research use only.

6-Gingerol is the active constituent of fresh ginger known to exhibit a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.

6-Gingerol Chemical Structure

CAS No. 23513-14-6

Selleck's 6-Gingerol has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description 6-Gingerol is the active constituent of fresh ginger known to exhibit a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.
In vitro

6-gingerol treatment significantly reduces the cell viability of human colon cancer cell, LoVo, in a dose-dependent manner. 6-gingerol induces significant G2/M phase arrest and has slight influence on sub-G1 phase in LoVo cells. Levels of cyclin A, cyclin B1, and CDK1 are diminished; in contrast, levels of the negative cell cycle regulators p27Kip1 and p21Cip1 are increased in response to 6-gingerol treatment. In addition, 6-gingerol treatment elevates intracellular reactive oxygen species (ROS) and phosphorylation level of p53. 6-gingerol is effective in suppressing the transformation, hyperproliferation, and inflammatory processes that initiate and promote carcinogenesis, as well as the later steps of carcinogenesis, namely, angiogenesis and metastasis[1]. 6-gingerol has direct cytotoxic effects on cultures of tumor cells, such as colorectal cancer cells, HL-60 cells and breast cancer cells[2].

In vivo 6-gingerol inhibits tumor growth in several types of murine tumors, such as B16F1 melanomas, Renca renal cell carcinomas and CT26 colon carcinomas, established by inoculating tumor cells on the flanks of mice, although it does not lead to complete eradication of the tumors. 6-gingerol treatment of tumor-bearing mice causes massive infiltration of CD4 and CD8 T-cells and B220+ B-cells, but reduces the number of CD4+Foxp3+ regulatory T-cells. The CD8 tumor-infiltrating T lymphocytes in 6-gingerol-treated mice strongly express IFN-γ, a marker of activation of CTL CD107a cells, and chemokine receptors that are expressed on TH1 cells, such as CXCR3 and CCR5[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Colon cancer cell line LoVo
  • Concentrations: 1, 5, 10, and 15 μg/mL
  • Incubation Time: 24 h or 48 h
  • Method: Colon cancer cell line LoVo is maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% v/v fetal bovine serum, 1% nonessential amino acid, 1% L-glutamine, and 100 μg/mL penicillin/streptomycin at 37°C in a humidified atmosphere with 5% CO2. Cells are seeded in 10 cm Petri dishes at an initial density of 2×105 cells/mL and grown to approximately 80% confluence. Then, the cells are collected for the subsequent analyses including cell viability, flow cytometric analysis, and immunoblotting analysis. For 6-gingerol treatments, cells are starved for 24 hours (h) in serum-free DMEM and then incubated with 6-gingerol at a series of concentrations in serum-free DMEM (1, 5, 10, and 15 μg/mL) for 24 h or 48 h.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Female Balb/c and C57BL/6 mice (Mice areinoculated with 3.0×105 CT26 cells, 7×105 Renca cells [Balb/c], or 2.5×105 B16F1 cells [C57BL/6] in the left flank)
  • Dosages: 3 μg/ml
  • Administration: p.o.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 58 mg/mL
(197.01 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.39
Formula

C17H26O4

CAS No. 23513-14-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCC(CC(=O)CCC1=CC(=C(C=C1)O)OC)O

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