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Protocatechuic acid

Name: Protocatechuic acid
Brand: Selleck Chemicals
SKU: S3975
Rating: 5 (1 reviews)

Synonyms: 3,4-Dihydroxybenzoic acid, Protocatechuate

Catalog No.S3975 Purity: 99.99%

Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.

Protocatechuic acid Chemical Structure

CAS: 99-50-3

Selleck's Protocatechuic acid has been cited by 1 publication

Purity & Quality Control

Batch: S397501 DMSO] 30 mg/mL] false] ] ] false] ] ] false Purity: 99.99%

99.99

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Biological Activity

Description	Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.

In vitro
In vitro	Protocatechuic acid (PCA) inhibits cell migration and invasion at non-cytotoxic concentrations. It down-regulates the Ras/Akt/NF-κB pathway by targeting RhoB activation, which in turn leads to a reduction of MMP-mediated cellular events in cancer cells. In some cancer cells, PCA treatment suppresses cell adhesion and the production of interleukin (IL)-6, IL-8, vascular endothelial growth factor and the intercellular adhesion molecule-1, which would further attenuate angiogenic and metastatic actions^[1]. PCA will lower the expression of cleaved caspase-3 and decrease the rate of cardiomyocyte apoptosis induced by hypoxia/reoxygenation. PCA is proven to have the neuroprotective effects in PC12 cells^[2].
Cell Research	Cell lines	human gastric adenocarcinoma (AGS) cells
	Concentrations	1 mM
	Incubation Time	24, 48 and 72 h
	Method	Cells (1 × 10⁴ cells/mL) are seeded in 12 well plates and treated with PCA (1.0 mM) or dimethyl sulphoxide (DMSO) as control for 24, 48 and 72 h. The number of cells is measured by the Trypan blue dye exclusion assay.

In Vivo
In vivo	Protocatechuic acid (PCA) inhibits metastasis of B16/F10 melanoma cells to the liver in mice^[1]. PCA is seen to enhance learning and memory ability, and alleviate oxidative stress, apoptosis and glial proliferation following CIH exposure in rats. In addition, PCA administration also decreases the level of IL-1β in brain and increases the expression of BDNF and SYN. Its administration inhibits apoptosis by decreasing JNK/P38 phosphoryltion and the downstream target including c-fos, Bax and Cleaved Caspase-3 in the hippocampal and prefrontal cortex areas. PCA exerts significant neuroprotective effects in CIH rats^[2].
Animal Research	Animal Models	6-week-old C57/BL6 mice with B16/F10 melanoma
	Dosages	200 and 400 mg/kg
	Administration	oral administration

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06192758	Not yet recruiting	Prostate\|Cancer	Boston Scientific Corporation	January 2024	Not Applicable
NCT05706285	Recruiting	Opioid Use\|Post Operative Pain\|Pain Assessment Scales	Marmara University	September 30 2023	--

References

Chemical Information & Solubility

Molecular Weight	154.12	Formula	C₇H₆O₄
CAS No.	99-50-3	SDF	Download Protocatechuic acid SDF
Smiles	C1=CC(=C(C=C1C(=O)O)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 30 mg/mL ( (194.65 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

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In vivo
Batch:

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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