Sophoridine

Catalog No.S3895 Synonyms: 5-Epidihydrosophocarpine, Dihydro-5-episophocarpine

For research use only.

Sophoridine (5-Epidihydrosophocarpine, Dihydro-5-episophocarpine), a natural product obtained from medicinal plants, has a variety of pharmacological effects, including anti-cancer and anti-arrhythmia, and affects the immune and central nervous systems.

Sophoridine Chemical Structure

CAS No. 6882-68-4

Selleck's Sophoridine has been cited by 1 Publication

Purity & Quality Control

Related Other Products

Biological Activity

Description Sophoridine (5-Epidihydrosophocarpine, Dihydro-5-episophocarpine), a natural product obtained from medicinal plants, has a variety of pharmacological effects, including anti-cancer and anti-arrhythmia, and affects the immune and central nervous systems.
In vitro

Sophoridine kills cancer cells but has low cytotoxicity to normal cells. Pancreatic cancer cells are particularly sensitive. Sophoridine inhibits the proliferation of pancreatic cancer cells and induces cell cycle arrest at S phase and mitochondrial-related apoptosis. Moreover, Sophoridine induces a sustained activation of the phosphorylation of ERK and JNK. In addition, Sophoridine provokes the generation of reactive oxygen species (ROS) in pancreatic cancer cells[1]. Sophoridine also triggers significant down-regulated the expression of p27, CDK2, Survivin, Livin, Bcl-2, E2F1 and the transcriptional activity of FoxM1, NF-κb and AP-1, meanwhile, up-regulated the expression of caspase-3/8, p53, Smac, c-JNK and p38-MAPK. Sophoridine significantly inhibits ubiquitin-proteasome in tumor cells[2].

In vivo Sophoridine is an effective agent that can inhibit the growth of xenograft pancreatic tumors in vivo with well-tolerated toxicity[1]. Sophoridine attenuates intestinal inflammation in the experimental colitis model, which is associated with the downregulation of ICAM-1 gene expression[3].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Miapaca-2 and PANC-1; HPDE
  • Concentrations: 0, 10, 20, 40, 80, or 100 μM
  • Incubation Time: 24 h, 48 h, or 72 h
  • Method: Two human pancreatic cancer cell lines (Miapaca-2 and PANC-1) and pancreatic ductal epithelial cell line (HPDE) are treated with Sophoridine at different concentrations (0, 10, 20, 40, 80, or 100 μM) for different lengths of time (24 h, 48 h, or 72 h). Cell viability is determined by the CCK-8 assay.
Animal Research:[1]
  • Animal Models: BALB/c homozygous (nu/nu) nude mice (6 weeks, 18 g)
  • Dosages: 20 mg/kg or 40 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 248.36
Formula

C15H24N2O

CAS No. 6882-68-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CC2C3CCCN4C3C(CCC4)CN2C(=O)C1

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Sophoridine | Sophoridine supplier | purchase Sophoridine | Sophoridine cost | Sophoridine manufacturer | order Sophoridine | Sophoridine distributor