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Crocin Apoptosis related chemical

Name: Crocin
Brand: Selleck Chemicals
SKU: S4758
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S4758

Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.

Chemical Structure

Molecular Weight: 976.96

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.69%

99.69

Related Targets	Ras STAT TNF-alpha Bcl-2 Caspase PD-1/PD-L1 Ferroptosis p53 RIP kinase c-RET MDM2/MDMX Myc IAP OXPHOS PERK Thymidylate Synthase FKBP Heme Oxygenase PFKFB PKD Survivin Pyroptosis ASK Bcl-6 Nur77 DAPK TRADD cytohesin TACE Cuproptosis
Related Products	Importazole Genipin Plumbagin 6-Gingerol Colcemid Euphorbia factor L3 Sophoridine Pomolic acid Flavone Protocatechuic acid Bisdemethoxycurcumin (BDMC)

Chemical Information, Storage & Stability

Molecular Weight	976.96	Formula	C₄₄H₆₄O₂₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	42553-65-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Alpha-Crocin, Gardenia Yellow			Smiles	CC(=CC=CC=C(C)C=CC=C(C)C(=O)OC1C(C(C(C(O1)COC2C(C(C(C(O2)CO)O)O)O)O)O)O)C=CC=C(C)C(=O)OC3C(C(C(C(O3)COC4C(C(C(C(O4)CO)O)O)O)O)O)O

Solubility

In vitro
Batch:

DMSO : 100 mg/mL ( (102.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5mg/ml (5.12mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Crocin can significantly inhibit the proliferation of MCF-7 cells, and induce their apoptosis through mitochondrial signaling pathways including the activation of Caspase-8, upregulation of Bax, the disruption of mitochondrial membrane potential (MMP), and the release of cytochrome c^[1]. This compound decreases cell viability in DLA (Daltons lymphoma) cells, in a concentration- and time-dependent manner^[2].
In vivo	Crocin exhibits a variety of pharmacological effects in mice including inhibition of skin tumor growth, improvement of learning behavior previously impaired by ethanol, anti-hyperlipidemic effects, therapeutic efficacy for colon adenocarcinomas in rats, anti-atherosclerotic effects and anti-oxidant effects in PC-12 cells by increasing GSH synthesis. For Daltons lymphoma models, The lifespan of ascities tumor bearing mice treated with this compound, are found to be significantly increasing and a significant reduction in solid tumour volume is found in crocin treated animals when compared to that of control animals. This chemical also shows significant impact on hematological parameters, like the hemoglobin count and numbers of lymphocytes^[2]. It has shown that this compound exhibits beneficial effects on many organs including the nervous system, the most studied, gastrointestinal, cardiovascular, genital, endocrine, immune systems, etc. At pharmacological doses it does not harm liver and other organs' function^[3].
References	https://pubmed.ncbi.nlm.nih.gov/26884946/ https://pubmed.ncbi.nlm.nih.gov/20104983/ https://pubmed.ncbi.nlm.nih.gov/24275090/

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