Crocin

Catalog No.S4758 Synonyms: Alpha-Crocin, Gardenia Yellow

For research use only.

Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.

Crocin Chemical Structure

CAS No. 42553-65-1

Selleck's Crocin has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavoring and coloring food preparations, and in Chinese traditional medicine as an anodyne or tranquilizer.
In vitro

Crocin can significantly inhibit the proliferation of MCF-7 cells, and induce their apoptosis through mitochondrial signaling pathways including the activation of Caspase-8, upregulation of Bax, the disruption of mitochondrial membrane potential (MMP), and the release of cytochrome c[1]. Crocin decreases cell viability in DLA (Daltons lymphoma) cells, in a concentration- and time-dependent manner[2].

In vivo Crocin exhibits a variety of pharmacological effects in mice including inhibition of skin tumor growth, improvement of learning behavior previously impaired by ethanol, anti-hyperlipidemic effects, therapeutic efficacy for colon adenocarcinomas in rats, anti-atherosclerotic effects and anti-oxidant effects in PC-12 cells by increasing GSH synthesis. For Daltons lymphoma models, The lifespan of ascities tumor bearing mice treated with crocin, are found to be significantly increasing and a significant reduction in solid tumour volume is found in crocin treated animals when compared to that of control animals. Crocin also shows significant impact on hematological parameters, like the hemoglobin count and numbers of lymphocytes[2]. It has shown that crocin exhibits beneficial effects on many organs including the nervous system, the most studied, gastrointestinal, cardiovascular, genital, endocrine, immune systems, etc. At pharmacological doses it does not harm liver and other organs' function[3].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: MCF-7 cells
  • Concentrations: 10, 25, 50 µg/ml
  • Incubation Time: 24 h
  • Method:

    MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.

  • (Only for Reference)
Animal Research:

[1]

  • Animal Models: Swiss albino mice injected 1 × 106 Daltons lymphoma cells to the peritoneal cavity
  • Dosages: 10 ml/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(102.35 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 976.96
Formula

C44H64O24

CAS No. 42553-65-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=CC=CC=C(C)C=CC=C(C)C(=O)OC1C(C(C(C(O1)COC2C(C(C(C(O2)CO)O)O)O)O)O)O)C=CC=C(C)C(=O)OC3C(C(C(C(O3)COC4C(C(C(C(O4)CO)O)O)O)O)O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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