Smoothened Agonist (SAG) HCl
Molecular Weight(MW): 526.52
Smoothened Agonist (SAG) HCl is a cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells.
Cited by 16 Publications
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Activating Shh signaling by SAG injection in naïve mice significantly increased BDNF expression, and pretreatment with cyclopamine effectively prevented the upregulation of BDNF induced by SAG (E). Tissues were collected at 2 hours after SAG injection. Six mice were included in each group.
J Pain Res, 2018, 11:649-659. Smoothened Agonist (SAG) HCl purchased from Selleck.
Purity & Quality Control
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|Description||Smoothened Agonist (SAG) HCl is a cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells.|
SAG regulates Smo activity by binding directly to the Smo heptahelical bundle.  SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way.  SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. 
|In vivo||In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells.  In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. |
|In vitro||Water||100 mg/mL (189.92 mM)|
|DMSO||71 mg/mL warmed (134.84 mM)|
|Ethanol||40 mg/mL warmed (75.97 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|CAS No.||912545-86-9(free base)|
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03645044||Recruiting||--||Hiv|HBV Coinfection||University of Melbourne|National Health and Medical Research Council Australia|The University of Western Australia|University of Adelaide|The HIV Netherlands Australia Thailand Research Collaboration|University of Malaya|YR Gaitonde Centre for AIDS Research and Education|Melbourne Health||May 24 2018||--|
|NCT03180619||Active not recruiting||Drug: TAF||Chronic Hepatitis B||Gilead Sciences||June 29 2017||Phase 2|
|NCT02195518||Completed||Drug: Tenofovir disoproxil fumarate||Hepatitis B Chronic||GlaxoSmithKline||March 18 2015||Phase 4|
|NCT02364336||Completed||Drug: Peginterferon alfa-2a||Chronic Hepatitis B||National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)|National Institutes of Health Clinical Center (CC)||February 14 2015||Phase 2|
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