Propranolol HCl

Catalog No.S4076 Synonyms: AY-64043, ICI-45520, NCS-91523

For research use only.

Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor with IC50 of 12 nM.

Propranolol HCl Chemical Structure

CAS No. 318-98-9

Selleck's Propranolol HCl has been cited by 8 Publications

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Biological Activity

Description Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor with IC50 of 12 nM.
Targets
β-adrenergic receptor [1]
12 nM
In vitro

Propranolol, a non-selective β1 and β2 adrenergic blocker, has no effect on the proliferation of neonatal mouse cardiac fibroblasts (NMCFs).[2]

Assay
Methods Test Index PMID
Growth inhibition assay Cell viability 27582542
Western blot Bax / Bcl-2 / Cyt C / Caspase-9 / Cleaved Caspase-9 / Caspase-3 / Cleaved Caspase-3 ; B-Raf / p-B-Raf / MEK / p-MEK / ERK / p-ERK / AKT / p-AKT 27582542
In vivo

Propranolol, an anti-arrhythmic drugs, consistently reduces the heart rates of adult mice by approximately 10%-20%, whereas isoproterenol has the opposite effect.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: neonatal mice cardiomyocytes (NMCMs)
  • Concentrations: 10, 20, 40, 50, and 100 μM
  • Incubation Time: 6, 12, 18, and 24 h
  • Method:

    In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.

Animal Research:

[2]

  • Animal Models: 1-day-old neonatal mice; 12-week-old adult mice
  • Dosages: 10 mg/kg/d
  • Administration: s.c.; ALZET Osmotic Pumps

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 295.8
Formula

C16H21NO2.HCl

CAS No. 318-98-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)NCC(COC1=CC=CC2=CC=CC=C21)O.Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04518124 Recruiting Drug: Propranolol Angiosarcoma The Netherlands Cancer Institute|Anticancer Fund Belgium December 27 2019 Phase 2
NCT03926663 Unknown status Drug: Dexmedetomidine|Drug: Bupivacaine Intranasal Dexmedetomidine|Intranasal Bupivacaine|Septoplasty Surgeries Cairo University July 20 2019 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-11-29)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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