Name: Propranolol HCl
Brand: Selleck Chemicals
SKU: S4076
Rating: 5 (13 reviews)

Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor.

Propranolol HCl Chemical Structure

CAS: 318-98-9

Selleck's Propranolol HCl has been cited by 13 publications

Purity & Quality Control

Batch: Purity: 99.78%

99.78

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Related Compound Libraries	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ GPCR Compound Library	Click to Expand

Signaling Pathway

Adrenergic Receptor Signaling Pathway

Choose Selective Adrenergic Receptor Inhibitors

Biological Activity

Description

Propranolol HCl (AY-64043, ICI-45520, NCS-91523) is a competitive non-selective beta-adrenergic receptors inhibitor.

Targets

β-adrenergic receptor ^[1]

12 nM

In vitro
In vitro	Propranolol, a non-selective β1 and β2 adrenergic blocker, has no effect on the proliferation of neonatal mouse cardiac fibroblasts (NMCFs).^[2]
Cell Research	Cell lines	neonatal mice cardiomyocytes (NMCMs)
	Concentrations	10, 20, 40, 50, and 100 μM
	Incubation Time	6, 12, 18, and 24 h
	Method	In vitro, the neonatal mice cardiomyocytes (NMCMs) are treated with different drugs of different concentration, including isoproterenol (0, 1, 2.5, 5, 10, 20, and 50 μM); amiodarone (0, 1, 2.5, 5, 10, 20, and 50 μM); metoprolol (0, 10, 20, 30, 50, 100, 150, and 200 μM); propranolol (0, 10, 20, 40, 50, and 100 μM); lidocaine (0, 1, 2.5, 5, 10, 20, and 50 μM); verapamil(0, 1, 2.5, 5, 10, 20, and 50 μM);ivabradine (0, 1, 2, 3, 5, 10, and 20 μM). And the concentrations of drugs that promote CMs proliferation most significantly are chosen for subsequent experiments, including isoproterenol (10 μM), amiodarone (5 μM), metoprolol (20 μM), propranolol (20 μM), lidocaine (5 μM), verapamil (2.5 μM), ivabradine (3 μM) for NMCMs, NMCFs, and hPSC-CM.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Growth inhibition assay	Cell viability		27582542
	Western blot	Bax / Bcl-2 / Cyt C / Caspase-9 / Cleaved Caspase-9 / Caspase-3 / Cleaved Caspase-3 B-Raf / p-B-Raf / MEK / p-MEK / ERK / p-ERK / AKT / p-AKT		27582542

In Vivo
In vivo	Propranolol, an anti-arrhythmic drugs, consistently reduces the heart rates of adult mice by approximately 10%-20%, whereas isoproterenol has the opposite effect.^[2]
Animal Research	Animal Models	1-day-old neonatal mice; 12-week-old adult mice
	Dosages	10 mg/kg/d
	Administration	s.c.; ALZET Osmotic Pumps

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06145074	Not yet recruiting	Pancreatic Cancer\|Surgery	Zealand University Hospital\|Rigshospitalet Denmark	January 1 2024	Phase 2
NCT05968690	Recruiting	Advanced Melanoma	Ryan Stephenson\|Rutgers The State University of New Jersey	September 11 2023	Phase 1
NCT05679193	Recruiting	Prostate Cancer	Oslo University Hospital	January 2 2023	Phase 2

References

Chemical Information & Solubility

Molecular Weight	295.8	Formula	C₁₆H₂₁NO₂.HCl
CAS No.	318-98-9	SDF	Download Propranolol HCl SDF
Smiles	CC(C)NCC(COC1=CC=CC2=CC=CC=C21)O.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 59 mg/mL ( (199.45 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 6 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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