Higenamine hydrochloride

Catalog No.S3960 Synonyms: Norcoclaurine HCl, (+-)-Demethylcoclaurine hydrochloride

For research use only.

Higenamine (Norcoclaurine, (+-)-Demethylcoclaurine), also known as Norcoclaurine HCl, is a non-selective β2 adrenoceptor agonist which is a chemical compound naturally occurring in a number of plants.

Higenamine hydrochloride Chemical Structure

CAS No. 11041-94-4

Selleck's Higenamine hydrochloride has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description Higenamine (Norcoclaurine, (+-)-Demethylcoclaurine), also known as Norcoclaurine HCl, is a non-selective β2 adrenoceptor agonist which is a chemical compound naturally occurring in a number of plants.
Targets
β2-adrenoceptor [1]
()
α1-adrenoceptor [1]
()
In vitro

Higenamine is a potential β2-adrenoceptor agonist. As a potent cardiotonic and vasodilator, It is also an α1 antagonist and a weak α2 agonist[1]. Higenamine decreases the intracellular dopamine content dose-dependently and shows 55.2% inhibition of dopamine content in PC12 cells at a concentration of 2OM with 24h incubation. The IC50 value of higenamine for inhibiting dopamine biosynthesis in PC12 cells is 18.2 μM. Dopamine content is lowered and reaches minimal level at 12—24h after exposure to higenamine[2].

In vivo Higenamine can produce relaxation in tracheal muscle[1]. Higenamine has anti-thrombotic effects in both mouse acute thrombosis model and rat arterio-venous shunt (AV-shunt) models. The oral administration of higenamine (50 or 100 mg/kg) increases the recovery rates from the acute thrombotic challenge in mice and lowers the weight of thrombus formed inside the AV-shunt tube in rats[3].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: PC12 cells
  • Concentrations: 5-20 μM
  • Incubation Time: 12-48 h
  • Method:

    PC12 cells are maintained routinely in RPMI 1640 medium supplemented with 10% heat-inactivated horse serum and 5 % fetal calf serum pius 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C. The PC12 cells (ca. 1×105 cells/cm2) are treated with higenamine (5-2O μM) and then incubated for 12 (-48) h. The cells (ca. 1.5-2 × 105 cells/cm2) are harvested with phosphate buffered saline (PBS) and the pellet extract is used for the measurement of dopamine content, and TH and AADC activities.

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: mice
  • Dosages: 50 or 100 mg/kg
  • Administration: oral
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 61 mg/mL
(198.19 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 307.77
Formula

C16H17NO3.HCl

CAS No. 11041-94-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CNC(C2=CC(=C(C=C21)O)O)CC3=CC=C(C=C3)O.Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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