L755507

Catalog No.S7974

For research use only.

L-755,507 is characterized as a potent and selective β3 adrenergic receptor partial agonist with EC50 of 0.43 nM. It is also recently identified to enhance CRISPR-mediated homology-directed repair (HDR) efficiency in human induced pluripotent stem cells (iPSCs) and other cell types.

L755507 Chemical Structure

CAS No. 159182-43-1

Selleck's L755507 has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description L-755,507 is characterized as a potent and selective β3 adrenergic receptor partial agonist with EC50 of 0.43 nM. It is also recently identified to enhance CRISPR-mediated homology-directed repair (HDR) efficiency in human induced pluripotent stem cells (iPSCs) and other cell types.
Targets
β3 adrenergic receptor [1]
()
0.43 nM(EC50)
In vitro

L-755,507 displays an excellent activity profile as an extremely potent human β3 adrenergic receptor agonist (β3 EC50 0.43 nM), with >440-fold selectivity over β1 and β2 binding[1]. L755507 causes a robust concentration-dependent increase in cAMP accumulation in CHO-K1 cells expressing human β3-adrenoceptors(pEC50 values of 12.3)[4]. In a recent study employing a high-throughput screen to identify chemicals capable of modulating HR-mediated genome editing, L-755507 is identified that could enhance HR repair by up to ninefold[3].

In vivo Acute exposure of rhesus monkeys to L-755,507 elicits lipolysis and metabolic rate elevation, and that chronic exposure increases uncoupling protein 1 expression in rhesus brown adipose tissue[2].

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: cancer cell lines(K562 and HeLa), suspension cells (K562), primary neonatal cells (HUVEC and fibroblast CRL-2097), and human ESC derived cells (neural stem cells)
  • Concentrations: 5 μM
  • Incubation Time: 24 hours
  • Method:

    --

Animal Research:

[2]

  • Animal Models: Male lean rhesus monkeys
  • Dosages: 3 mg/kg
  • Administration: i.v.

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(171.01 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 584.73
Formula

C30H40N4O6S

CAS No. 159182-43-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCCCCNC(=O)NC1=CC=C(C=C1)S(=O)(=O)NC2=CC=C(C=C2)CCNCC(COC3=CC=C(C=C3)O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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