Plerixafor (AMD3100)

For research use only.

Catalog No.S8030 Synonyms: JM 3100

59 publications

Plerixafor (AMD3100) Chemical Structure

CAS No. 110078-46-1

Plerixafor (AMD3100, JM 3100) is a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor inhibits human immunodeficiency virus (HIV) replication.

Size Price Stock Quantity  
USD 97 In stock
USD 147 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Plerixafor (AMD3100) has been cited by 59 publications

Purity & Quality Control

Choose Selective CXCR Inhibitors

Biological Activity

Description Plerixafor (AMD3100, JM 3100) is a chemokine receptor antagonist for CXCR4 and CXCL12-mediated chemotaxis with IC50 of 44 nM and 5.7 nM in cell-free assays, respectively. Plerixafor inhibits human immunodeficiency virus (HIV) replication.
Targets
CXCL12 [1]
(Cell-free assay)
CXCR4 [1]
(Cell-free assay)
5.7 nM 44 nM
In vitro

Plerixafor inhibits CXCL12-mediated chemotaxis with a potency lightly better than its affinity for CXCR4. [1] Plerixafor also antagonizes SDF-1/CXCL12 ligand binding with an IC50 of 651 nM. Plerixafor inhibits SDF-1 mediated GTP-binding, SDF-1 mediated calcium flux and SDF-1 stimulated chemotaxis with IC50 of 27 nM, 572 nM and 51 nM, respectively. Plerixafor does not inhibit calcium flux against cells expressing CXCR3, CCR1, CCR2b, CCR4, CCR5 or CCR7 when stimulated with their cognate ligands, nor does Plerixafor inhibit receptor binding of LTB4. Plerixafor does not, on its own, induce a calcium flux in the CCRF–CEM cells, which express multiple GPCRs including CXCR4, CCR4 and CCR7. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHOK1 M2PjUWZ2dmO2aX;uJIF{e2G7 MUTEbZNxdGGlZX3lcpQhd2ZiW{GyOWleW0SIMXHsdIhiKG[{b32gR2NTOi:FWFPSOEBmgHC{ZYPz[YQhcW5iQ1jPT|Eh[2WubIOsJGlEPTBiPTCwMlAxODB2IN88UU4> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdzNUGyPEc,OTd5MUWxNlg9N2F-
CHOK1 M{WyN2Z2dmO2aX;uJIF{e2G7 NY\YUlVRTGm|cHzhZ4Vu\W62IH;mJHsyOjWLXV3DVFEh\nKxbTDDR3IzN0O[Q2K0JIV5eHKnc4Pl[EBqdiCFSF;LNUBk\WyuczygTWM2OCB;IECuNFAxODlizszNMi=> NYq0fXY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NVUyOjhpPkG3O|E2OTJ6PD;hQi=>
CHOK1 NGnpWYdHfW6ldHnvckBie3OjeR?= MnfSSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZVOGRkHhcJBp[SCocn;tJGNZS1J2IHX4dJJme3OnZDDpckBEUE:NMTDj[YxteyxiSVO1NEA:KDBwMECwPFEh|ryPLh?= NHLUWlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{exOVEzQCd-MUe3NVUyOjh:L3G+
U87.CD4 MUjBcpRqfmm{YXygZZN{[Xl? NWX2WHI{SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQ{KGmwZnXjeIVlKGmwIGW4O{5ETDRiY3XscJMh\XiycnXzd4lv\yCqdX3hckBEYEOUNDDINlgySSCvdYThcpQtKEmFNUCgQUAxNjByMUmg{txONg>? NEnDem09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
U87.CD4 MVPBcpRqfmm{YXygZZN{[Xl? MlX1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQRFugbY5n\WO2ZXSgbY4hXTh5LlPEOEBk\WyuczDlfJBz\XO|aX7nJIh2dWGwIFPYR3I1KEh{OEHBJI12fGGwdDygTWM2OCB;IECuNFAyQSEQvF2u NHSz[Wk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
MT4 NX;3UXF4SW62aY\pdoFtKGG|c3H5 M1zVelUh\GG7cx?= MXHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5idnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjC1JIRigXNiYomgUXRVKGG|c3H5MEBGSzVyIE2gNE4xODJizszNMi=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl5NEO3Okc,OjZ7N{SzO|Y9N2F-
MT4 NX\qV3NOSW62aY\pdoFtKGG|c3H5 NYTTcWdKPSCmYYnz MkfiRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOiCUT1SgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGGodHXyJFUh\GG7czDifUBOXFRiYYPzZZktKEWFNUCgQUAxNjByMjFOwG0v MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl5NEO3Okc,OjZ7N{SzO|Y9N2F-
HEK293 MUHBcpRqfmm{YXygZZN{[Xl? MYKyJIRigXN? NHi5ZllCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBVOjBvcnXzbZN1[W62IFjJWlEhVkx2LUOgbY5n\WO2ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhemWybHnjZZRqd25iYX\0[ZIhOiCmYYnzMEBKSzVyIE2gNE4xODJ|IN88UU4> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
U87.CD4 NVLJUJpLSW62aY\pdoFtKGG|c3H5 MYXBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKGOuaX7pZ4FtKGm|b3zheIUhOTBiaX7m[YN1\WRiaX6gWVg4NkOGNDDj[YxteyCneIDy[ZN{cW6pIHj1cYFvKEO[Q2K0JGgzQDGDIH31eIFvfCxiSVO1NEA:KDBwMECyOEDPxE1w MlvHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
U87.CD4 M3LS[WFvfGm4aYLhcEBie3OjeR?= NXjadGo4SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDjcIlvcWOjbDDpd49t[XSnIEGwJIlv\mWldHXkJIlvKFV6Nz7DSFQh[2WubIOg[ZhxemW|c3nu[{BpfW2jbjD3bYxlKHS7cHWgR3hEWjRuIFnDOVAhRSByLkCwN{DPxE1w NUHP[2JyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
PBMC NWDOWFAzTnWwY4Tpc44h[XO|YYm= MUHF[oZm[3SrdnWgZ49v[2WwdILheIlwdiCxZjDjc41xd3WwZDDh[4FqdnO2IFjJWk0yKDh7Lk[gd5Rz[WmwIHnuJHBDVUNiY3XscJMtKEWFNUCgQUAxNjByM{ig{txONg>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDZ7OEG4PUc,OTR4OUixPFk9N2F-
MT4 MV;BcpRqfmm{YXygZZN{[Xl? MkmxOEBl[Xm| M13YbWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQ1KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNicnXwcIlk[XSrb36gZYZ1\XJiNDDkZZl{KGK7IF3UWEBie3OjeTygSWM2OCB;IECuNFA1KM7:TT6= NX3FTnJkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwOFM3OzhpPkKwNFQ{PjN6PD;hQi=>
MT-4 NWnpVpAyTnWwY4Tpc44h[XO|YYm= M{XNNGVn\mWldHn2[UBkd26lZX70doF1cW:wIHHnZYlve3RiSFnWMVEpUUmLQjmgdoVxdGmlYYTpc44hcW5iTWStOEBk\WyuczygSWM2OCB;IECuNFA1OiEQvF2u NFXkdJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96NU[4O|k4Lz56NU[4O|k4RC:jPh?=
HEK293 NHPWcmtCdnSrdnnyZYwh[XO|YYm= Ml2yNkBl[Xm| NIj5RlRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIF7MOE0{KGmwZnXjeIVlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEKg[IF6eyxiSVO1NEA:KDBwMEC0OkDPxE1w NH\0PGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
HEK293 MonKRY51cX[rcnHsJIF{e2G7 MlrWNkBl[Xm| MWLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDteYx1cWS{dXegdoV{cXO2YX70JGhKXjFiSGjCNkBqdm[nY4Tl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB{IHThfZMtKEmFNUCgQUAxNjByNUOg{txONg>? NF7SR449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
MT-4 MlG2SpVv[3Srb36gZZN{[Xl? NXSwR3ZMTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gZYdicW6|dDDITXYuOiiUT1SpJJJmeGyrY3H0bY9vKGmwIF3UMVQh[2WubIOsJGVEPTBiPTCwMlAxPTlizszNMi=> Mn3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDV4OEe5O{c,QDV4OEe5O|ww[T5?
CEM-CCRF M2PyPWZ2dmO2aX;uJIF{e2G7 M2TUclMxKG2rboO= MlTITY5pcWKrdHnvckBw\iCSRT3jc45rfWejdHXkMVEzTzViYX70bU1EYEOUNDDhcpRq[m:meTDibY5lcW6pIITvJGNZS1J2IHnuJIh2dWGwIFPFUU1ES1KIIHPlcIx{KHC{ZXnuZ5Vj[XSnZDDmc5IhOzBibXnud{Bnd2yub4fl[EBjgSCjboTpZo9lgSCjZHTpeIlwdiCkeTDGRWNUKEOjboTvJGlKKGO7dH;mcJVwem:vZYTybYMh[W6jbInzbZMtKEmFNUCgQUAxNjByNjFOwG0v MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV5MUCzPEc,Ojd3N{GwN|g9N2F-
HEK293 M2XWTmFvfGm4aYLhcEBie3OjeR?= MVOyJIRigXN? M3rMSmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiSGjCNkBqdm[nY4Tl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB{IHThfZMtKEmFNUCgQUAxNjByNkKg{txONg>? M3\3WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
HEK293 M3rme2FvfGm4aYLhcEBie3OjeR?= NXLN[3ByOiCmYYnz MYrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDOUnJVUS2{ZYPpd5RidnRiSFnWNUBJYEJ{IHnu[oVkfGWmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJJJmeGyrY3H0bY9vKGGodHXyJFIh\GG7czygTWM2OCB;IECuNFA4KM7:TT6= MlfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGzNFUoRjF7NEWxN|A2RC:jPh?=
U87.CD4 NFH5VGVCdnSrdnnyZYwh[XO|YYm= NXjheZE6SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOSGshcW6oZXP0[YQhcW5iVUi3MmNFPCClZXzsd{BmgHC{ZYPzbY5oKGi3bXHuJJdqdGRidInw[UBEYEOUNDygTWM2OCB;IECuNFA4PiEQvF2u NUDz[WZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
MT-4 NXPxS4dRSW62aY\pdoFtKGG|c3H5 NHrqbIVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZiMTDSTW4hUEmYIEGgVmlPKGijcnLvdolv\yCrboTl[5Jie2ViZ3Xu[UBqdm[nY4Tl[EBqdiCPVD20JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBjgSCPVGSgZZN{[XliYX\0[ZIhPDBicHHzd4Fo\XNic3Xs[YN1\WRiaX6gdJJme2WwY3Wgc4Yh[2:vcH;1coQtKEWFNUCgQUAxNjByODFOwG0v MmnDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|N{i3NVMoRjF6M{e4O|E{RC:jPh?=
MT-4 NGD6d5dCdnSrdnnyZYwh[XO|YYm= MnHaRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXKDFiUlnOJGhKXiBzIGLJUkBp[XKkb4LpcochcW62ZXfyZZNmKGenbnWgbY5n\WO2ZXSgbY4hVVRvNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZpkhVVSWIHHzd4F6KGGodHXyJFYxKHCjc4Ph[4V{KHOnbHXjeIVlKGmwIIDy[ZNmdmOnIH;mJINwdXCxdX7kMEBGSzVyIE2gNE4xODhizszNMi=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN5OEexN{c,OTh|N{i3NVM9N2F-
MT-4 NUDFZW1QSW62aY\pdoFtKGG|c3H5 NFvDVHBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZiMTDSTW4hcGG{Yn;ybY5oKGmwdHXndoF{\SCpZX7lJIlv\mWldHXkJIlvKE2WLUSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIJ6KE2WVDDhd5NigSCjZoTldkAzOCCyYYPzZYdmeyC|ZXzlZ5Rm\CCrbjDwdoV{\W6lZTDv[kBkd22yb4Xu[EwhTUN3MDC9JFAvODB6IN88UU4> Mk\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|N{i3NVMoRjF6M{e4O|E{RC:jPh?=
HEK293 NG\XT3FCdnSrdnnyZYwh[XO|YYm= MknHNkBl[Xm| NYTEXYhuSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhVlKWST3y[ZNqe3SjboSgTGlXOSCKWFKyJIlv\mWldHXkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHHmeIVzKDJiZHH5d{whUUN3MDC9JFAvODB7IN88UU4> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
HEK293 MlzBRY51cX[rcnHsJIF{e2G7 M4OxXVIh\GG7cx?= M2fQXmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHBKNXKnc3nzeIFvfCCKSW[xJGhZSjJiaX7m[YN1\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgNkBl[Xm|LDDJR|UxKD1iMD6wNFkzKM7:TT6= MkDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGzNFUoRjF7NEWxN|A2RC:jPh?=
U87.CD4 MW\BcpRqfmm{YXygZZN{[Xl? MmPlRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCQTESzJIlv\mWldHXkJIlvKFV6Nz7DSFQh[2WubIOg[ZhxemW|c3nu[{BpfW2jbjD3bYxlKHS7cHWgR3hEWjRuIFnDOVAhRSByLkCxOEDPxE1w MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
MT-4 MnjVRY51cX[rcnHsJIF{e2G7 MoTTRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXKDFiUlnOJIhiemKxcnnu[{BqdnSnZ4Lhd4Uh\2WwZTDpcoZm[3SnZDDpckBOXC12IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{dYOtbY5lfWOnZDDjfZRweGG2aHnjJIVn\mWldDDifUBOXFRiYYPzZZktKEWFNUCgQUAxNjBzNDFOwG0v M4TYW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{e4O|E{Lz5zOEO3PFcyOzxxYU6=
U87.CD4 MX3BcpRqfmm{YXygZZN{[Xl? MYnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKE6GSzDpcoZm[3SnZDDpckBWQDdwQ1S0JINmdGy|IHX4dJJme3OrbnegbJVu[W5iQ2jDVlQhTDF5MV6gcZV1[W62LDDJR|UxKD1iMD6wNVch|ryPLh?= MnGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
CD4+ T NHXTe|VHfW6ldHnvckBie3OjeR?= MkDsRY51[WexbnnzeEBi[3Srdnn0fUBifCCFWFPSOEBqdiCqdX3hckBETDRtIGSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFWFPMNVIudWWmaXH0[YQh[3m2b4PvcIlkKGOjbHPpeY0hdGW4ZXygdJJmcW6ldXLheIVlKHerdHigZ49ueG:3bnTzJIZwdGyxd3XkJIJ6KEO[Q1yxNkB{fGmvdXzheIlwdiCkeTDjZYxkcXWvIESg[JlmNWKjc3XkJGZNUVCUIHHzd4F6NCCLQ{WwJF0hOC5yMUig{txONg>? M4rQelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEm0PFQ{Lz5{OUS5OFg1OzxxYU6=
U87.CD4 MnS1RY51cX[rcnHsJIF{e2G7 NH\2fHVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHPsbY5q[2GuIHnzc4xifGViMUCgbY5n\WO2ZXSgbY4hXTh5LlPEOEBk\WyuczDlfJBz\XO|aX7nJIh2dWGwIFPYR3I1KERzN{HOJI12fGGwdDygTWM2OCB;IECuNFE6KM7:TT6= MnvhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
MT4 NYnmZYpVSW62aY\pdoFtKGG|c3H5 MXK1JIRigXN? NF;1ZlBCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBVNWOnbHygcIlv\S22cn;wbYMhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYToc4dmdmmlaYT5JIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiNTD1UUBw\iClaHzvdo9yfWmwZTygSWM2OCB;IECuNFI2KM7:TT6= NHP0TWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC5OFk1PCd-Mk[wPVQ6PDR:L3G+
Jurkat M1[5ZmZ2dmO2aX;uJIF{e2G7 M{HhSmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiQ2jDVlQhcW5iaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiU1TGNU1qdmS3Y3XkJINmdGxibXnndoF1cW:wLDDJR|UxKD1iMD6wNlc1KM7:TT6= M1vwXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUi4NFcyLz5zOUG4PFA4OTxxYU6=
MT-4 NVPVS4k4SW62aY\pdoFtKGG|c3H5 M{njd2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXiBzIEPCJIhiemKxcnnu[{BqdnSnZ4Lhd4UhVDN2TTDteZRidnRiaX7m[YN1\WRiaX6gUXQuPCClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYomgUXRVKGG|c3H5JJNmdGWldHXkJIFnfGW{IESwJJBie3OjZ3XzJIlvKHC{ZYPlcoNmKG:oIHPvcZBwfW6mLDDFR|UxKD1iMD6wNlgh|ryPLh?= M1\NWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{e4O|E{Lz5zOEO3PFcyOzxxYU6=
MT4 MUPBcpRqfmm{YXygZZN{[Xl? MnfnOUBl[Xm| NECyT2JCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBVNWOnbHygcIlv\S22cn;wbYMhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYToc4dmdmmlaYT5JIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYmsJGVEPTBiPTCwMlA{OiEQvF2u NV;Cb4xlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPVQ6PDRpPkK2NFk1QTR2PD;hQi=>
MT-4 MmnKRY51cX[rcnHsJIF{e2G7 Mlv5RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXKDFiM1KgbY5n\WO2ZXSgbY4hVVRvNDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZpkhVVSWIHHzd4F6NCCHQ{WwJF0hOC5yM{Sg{txONg>? NWLDbIFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizO|g4OTNpPkG4N|c5PzF|PD;hQi=>
MT4 MXHBcpRqfmm{YXygZZN{[Xl? Ml:4OUBl[Xm| NGP1bW1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBVNWOnbHygcIlv\S22cn;wbYMhUEmYMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCPVESgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYToc4dmdmmlaYT5JIFnfGW{IEWg[IF6eyCkeTDNWHQh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiMj61JJVOKG:oIHPocI9zd3G3aX7lMEBGSzVyIE2gNE4xOzlizszNMi=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB7NEm0OEc,OjZyOUS5OFQ9N2F-
U87.CD4 NWjoOoo2SW62aY\pdoFtKGG|c3H5 NXzEXWY{SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDOUFQ{KGmwZnXjeIVlKGmwIGW4O{5ETDRiY3XscJMh\XiycnXzd4lv\yCqdX3hckBEYEOUNDDENVcyViCvdYThcpQtKEmFNUCgQUAxNjB2NjFOwG0v MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
MT-4 NEPqfWxCdnSrdnnyZYwh[XO|YYm= M1HmWmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXiBzIEPCJIhiemKxcnnu[{BqdnSnZ4Lhd4UhTTl{UTDTNlMxViCmb4XicIUhdXW2YX70JIlv\mWldHXkJIlvKE2WLUSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIJ6KE2WVDDhd5NigSC|ZXzlZ5Rm\CCjZoTldkAzOCCyYYPzZYdmeyCrbjDwdoV{\W6lZTDv[kBkd22yb4Xu[EwhTUN3MDC9JFAvODR7IN88UU4> M3GyW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{e4O|E{Lz5zOEO3PFcyOzxxYU6=
MT-4 MW\BcpRqfmm{YXygZZN{[Xl? NGrPSHJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZiMTCzRkBp[XKkb4LpcochcW62ZXfyZZNmKEV7MmGsJHMzOzCQIHHu[EBNOzSPIITybZBt\SCvdYThcpQhcW6oZXP0[YQhcW5iTWStOEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5Qh[nliTWTUJIF{e2G7IIPlcIVkfGWmIHHmeIVzKDZyIIDhd5Ni\2W|IHnuJJBz\XOnbnPlJI9nKGOxbYDveY5lNCCHQ{WwJF0hOC5yNU[g{txONg>? NVuwdGJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizO|g4OTNpPkG4N|c5PzF|PD;hQi=>
MT-4 MUjGeY5kfGmxbjDhd5NigQ>? NYLIbXhqTW[oZXP0bZZmKGOxbnPlcpRz[XSrb36gc4Yh[2:vcH;1coQh[WejaX7zeEBJUVZvMTDJTWlDKHO2cnHpckBqdiCPVD20JINmdGy|LDDFR|UxKD1iMD6wOlUh|ryPLh?= MkDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR4OUixPFkoRjF2Nkm4NVg6RC:jPh?=
HEK293 MVLGeY5kfGmxbjDhd5NigQ>? MkTYTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KEh{OEHBJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|UxKD1iMD6wO|I4KM7:TT6= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
IR983F NYTsblBZTnWwY4Tpc44h[XO|YYm= NGPIT21FcXOybHHj[Y1mdnRib3[gX|EzPUmfQ2jDUFEzKG[{b32gR3hEWjRiaX6gdoF1KEmUOUizSkBk\WyuczygTWM2OCB;IECuNVA5KM7:TT6= Mlv2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyNUO3OlgoRjF7MEWzO|Y5RC:jPh?=
CEM-SS NVH2elR7TnWwY4Tpc44h[XO|YYm= MmjRSYZn\WO2aY\lJINwdmOnboTyZZRqd25ib3[gZ49ueG:3bnSgZYdicW6|dDDITXYuOSCOQVmgd5Rz[WmwIHnuJGNGVS2VUzDj[YxteyxiRVO1NEA:KDBwMUK3JO69VS5? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDZ7OEG4PUc,OTR4OUixPFk9N2F-
COS7 MV;GeY5kfGmxbjDhd5NigQ>? NUGxW5l{SW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFWFPSOEBXOTl4QTDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[2:neIDy[ZN{cW6pIFegdJJwfGWrbjDHdYk1dXm{IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2jDUFEzNWmwZIXj[YQheGixc4DoZZRq\HmuaX7vd4l1d2xidIXyco93\XJuIFvpJF0hOC5zNDFOwG0v M1uwTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
HEK293 NIrjO|VHfW6ldHnvckBie3OjeR?= MYHJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9iQ2jDVlQhTDF6MVGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVAhRSByLkG0N|ch|ryPLh?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
COS7 NUDRUGZ6TnWwY4Tpc44h[XO|YYm= NHXtSJBFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFchd2ZiaIXtZY4hS1iFUkSgTFI5OUFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IECuNVYh|ryPLh?= NELreWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 MXzGeY5kfGmxbjDhd5NigQ>? Mlm5RY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBEYEOUNDDGNVczSSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZ49mgHC{ZYPzbY5oKEdicILveIVqdiCJcXm0cZlzKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1iFTEGyMYlv\HWlZXSgdIhwe3CqYYTp[JltcW6xc3n0c4whfHW{bn;2[ZItKEurIE2gNE4yPyEQvF2u NWrWd2VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
HEK293 NV:3ZW9[TnWwY4Tpc44h[XO|YYm= MmXTTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KFZ{OEDBJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|UxKD1iMD6xO|U{KM7:TT6= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
COS7 Mo[wSpVv[3Srb36gZZN{[Xl? M3jkdmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgTFI5OUFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJIIDyc5RmcW5iR4HpOI16eiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEO[Q1yxNk1qdmS3Y3XkJJBpd3OyaHH0bYR6dGmwb4PpeI9tKHS3cn7veoVzNCCNaTC9JFAvOTlizszNMi=> M4Puc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
HEK293 MV\GeY5kfGmxbjDhd5NigQ>? M2HsXGlvcGmkaYTpc44hd2ZiTXHiJFEzTzViYnnu[Ilv\yC2bzDDXGNTPCCYMUGyRUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM2OCB;IECuNVk3PiEQvF2u NHT3XZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
COS7 MXHGeY5kfGmxbjDhd5NigQ>? M1rZTGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hf2muZDD0fZBmKEO[Q2K0JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHPv[ZhxemW|c3nu[{BIKHC{b4TlbY4hT3GrNH35dkBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGNZS0xzMj3pcoR2[2WmIIDoc5NxcGG2aXT5cIlvd3OrdH;sJJR2em6xdnXyMEBMcSB;IECuNlIh|ryPLh?= NV\xXotiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
HEK293 NYjpN2l7TnWwY4Tpc44h[XO|YYm= MWXJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9iQ2jDVlQhTTJ5NVGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVAhRSByLkKzOVYh|ryPLh?= NUHrdnlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVE{ODVpPkG5OFUyOzB3PD;hQi=>
CEM M4G4fGZ2dmO2aX;uJIF{e2G7 Mmf2SIl{eGyjY3Xt[Y51KG:oIGuxNlVKZUO[Q1yxNkBnem:vIFPYR3I1KGmwIHj1cYFvKEOHTTDj[YxteyxiSVO1NEA:KDBwMkS1JO69VS5? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB3M{e2PEc,OTlyNUO3Olg9N2F-
HEK293 NV;GeYZHTnWwY4Tpc44h[XO|YYm= MnL3TY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KFZ7OVGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVAhRSByLkK1PFUh|ryPLh?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
HEK293 NVzRTJhCTnWwY4Tpc44h[XO|YYm= M3PwfWlvcGmkaYTpc44hd2ZiTXHiJFEzTzViYnnu[Ilv\yC2bzDDXGNTPCCKMkCzRUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM2OCB;IECuNlU6KM7:TT6= NEewdY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
HEK293 M3GzcGZ2dmO2aX;uJIF{e2G7 M2HLXWlvcGmkaYTpc44hd2ZiTXHiJFEzTzViYnnu[Ilv\yC2bzDDXGNTPCCLMki0RUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM2OCB;IECuNlY2QCEQvF2u M4[2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
HEK293 MYjGeY5kfGmxbjDhd5NigQ>? MVjJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9id3ns[EB1gXCnIFPYR3I1KGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMtKEmFNUCgQUAxNjJ6OUGg{txONg>? NXPXZmc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVE{ODVpPkG5OFUyOzB3PD;hQi=>
COS7 M320SWZ2dmO2aX;uJIF{e2G7 M{DQemFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgTFIxO0FibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJIIDyc5RmcW5iR4HpOI16eiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEO[Q1yxNk1qdmS3Y3XkJJBpd3OyaHH0bYR6dGmwb4PpeI9tKHS3cn7veoVzNCCNaTC9JFAvOjlizszNMi=> MlrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 MoHOSpVv[3Srb36gZZN{[Xl? M{frRWFvfGGpb37pd5Qh[WO2aY\peJkh[XRidILhcpNu\W2kcnHu[UBld22jaX6gOkBw\iCqdX3hckBEYEOUNDDJNlU6SSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZ49mgHC{ZYPzbY5oKEdicILveIVqdiCJcXm0cZlzKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1iFTEGyMYlv\HWlZXSgdIhwe3CqYYTp[JltcW6xc3n0c4whfHW{bn;2[ZItKEurIE2gNE4zQSEQvF2u NEL0OYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 NEDlS4tHfW6ldHnvckBie3OjeR?= NFvh[pRCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEO[Q2K0JHQzQDeDIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bkd2W6cILld5NqdmdiRzDwdo91\WmwIFfxbVRugXJiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFWFPMNVIucW6mdXPl[EBxcG:|cHjheIllgWyrbn;zbZRwdCC2dYLuc5ZmeixiS3mgQUAxNjJ7IN88UU4> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
HPBALL NYrvRllbTnWwY4Tpc44h[XO|YYm= M33yN|MhcHK| NGrnRlFFcXOybHHj[Y1mdnRib3[gNVJIPS2FWFPMNVIh\nKxbTDDXGNTPCCrbjDoeY1idiCKUFLBUGwh[2WubIOgZYZ1\XJiMzDodpMh[nliRlHDV{BidmGueYPpd{whUUN3MDC9JFAvOjlizszNMi=> MkWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OUS4OFMoRjJ7NEm0PFQ{RC:jPh?=
HEK293 NGjUWXFHfW6ldHnvckBie3OjeR?= MoTkTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KEhzMUPBJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|UxKD1iMD6yPVY1KM7:TT6= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
HEK293 NI\zb|lHfW6ldHnvckBie3OjeR?= M3nHZmlvcGmkaYTpc44hd2ZiTXHiJFEzTzViYnnu[Ilv\yC2bzDDXGNTPCCZMkizRUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM2OCB;IECuN|AxOiEQvF2u M4HzdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
CEM-SS NH;SVFFCdnSrdnnyZYwh[XO|YYm= NEfCS3hCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIFzBTUBqdiCqdX3hckBETU1vU2OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdixiSVO1NEA:KDBwM{Kg{txONg>? MmTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NU[4NlcoRjF7M{W2PFI4RC:jPh?=
COS7 MoHHSpVv[3Srb36gZZN{[Xl? MVnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNZS1J2IFyxNlBHKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczDjc4V5eHKnc4PpcochTyCycn;0[YlvKEezaUTtfZIh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBEYEOOMUKtbY5lfWOnZDDwbI9{eGijdHnkfYxqdm:|aYTvcEB1fXKwb4\ldkwhU2liPTCwMlM{KM7:TT6= MnHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 MnL1SpVv[3Srb36gZZN{[Xl? NUnhWnFYSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFWFPSOEBSOjByVzDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[2:neIDy[ZN{cW6pIFegdJJwfGWrbjDHdYk1dXm{IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2jDUFEzNWmwZIXj[YQheGixc4DoZZRq\HmuaX7vd4l1d2xidIXyco93\XJuIFvpJF0hOC52IN88UU4> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
MT4 M3H6VWFvfGm4aYLhcEBie3OjeR?= MWrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKDOEIHnuJIh2dWGwIF3UOEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wLDDJR|UxKD1iMD60NUDPxE1w M4\TdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{W2PFI4Lz5zOUO1OlgzPzxxYU6=
HEK293 NFK3WXhHfW6ldHnvckBie3OjeR?= MoWxTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KEV{N{fBJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|UxKD1iMD60Olk2KM7:TT6= MlmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGzNFUoRjF7NEWxN|A2RC:jPh?=
COS7 MV3GeY5kfGmxbjDhd5NigQ>? NEDiNXdFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFUhd2ZiaIXtZY4hS1iFUkSgVVIxOEFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IECuOVYh|ryPLh?= MnzZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
HEK293 MnvKSpVv[3Srb36gZZN{[Xl? NV;SZop6UW6qaXLpeIlwdiCxZjDNZYIhOTKJNTDibY5lcW6pIITvJGNZS1J2IFiyPFFCKG23dHHueEBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBKSzlyIE2gNE42PzJ{IN88UU4> NFH0Ro49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
COS7 MlqwSpVv[3Srb36gZZN{[Xl? NIjLbmRCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEO[Q2K0JGkzPTmZIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bkd2W6cILld5NqdmdiRzDwdo91\WmwIFfxbVRugXJiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFWFPMNVIucW6mdXPl[EBxcG:|cHjheIllgWyrbn;zbZRwdCC2dYLuc5ZmeixiS3mgQUAxNjZ|IN88UU4> NV\4PXJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
COS7 NWrodIVTTnWwY4Tpc44h[XO|YYm= M3noZ2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgXVI2PUFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJIIDyc5RmcW5iR4HpOI16eiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEO[Q1yxNk1qdmS3Y3XkJJBpd3OyaHH0bYR6dGmwb4PpeI9tKHS3cn7veoVzNCCNaTC9JFAvPjZizszNMi=> M3nqWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
COS7 MULGeY5kfGmxbjDhd5NigQ>? NWX1WYRnSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFWFPSOEBJOTF|QTDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[2:neIDy[ZN{cW6pIFegdJJwfGWrbjDHdYk1dXm{IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2jDUFEzNWmwZIXj[YQheGixc4DoZZRq\HmuaX7vd4l1d2xidIXyco93\XJuIFvpJF0hOC55NDFOwG0v MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
HEK293 NHP4OXpHfW6ldHnvckBie3OjeR?= MlvOTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIFPYR3I1KERzOEHBJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|kxKD1iMD63PVU3KM7:TT6= MleyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NUGzNFUoRjF7NEWxN|A2RC:jPh?=
HEK293 NFW5XVFHfW6ldHnvckBie3OjeR?= MYLJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9iQ2jDVlQhXjJ6MFGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDPVAhRSByLkiyNVIh|ryPLh?= M2fEN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEWxN|A2Lz5zOUS1NVMxPTxxYU6=
HEK293 NF23[ZBHfW6ldHnvckBie3OjeR?= MVjJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9iQ2jDVlQhXjFzMlGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDPVAhRSByLkiyNVMh|ryPLh?= NHnqblU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
HEK293 M3:2TGZ2dmO2aX;uJIF{e2G7 M1zwUWlvcGmkaYTpc44hd2ZiTXHiJFEzTzViYnnu[Ilv\yC2bzDDXGNTPCCKMkCzRUBufXSjboSg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM6OCB;IECuPFYxPiEQvF2u NFH3PJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS1NVMxPSd-MUm0OVE{ODV:L3G+
COS7 NHPCbGxHfW6ldHnvckBie3OjeR?= M2DiVWRqe3CuYXPlcYVvfCCxZjDbNVI2UV1zMle1JIFvfGmkb3T5JIZzd21iaIXtZY4hf2muZDD0fZBmKEO[Q2K0JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|LDDLbUA:KDBwOEmg{txONg>? NHnrPHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 MWnGeY5kfGmxbjDhd5NigQ>? NGDvdpFCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEO[Q2K0JHEzODCDIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{Bkd2W6cILld5NqdmdiRzDwdo91\WmwIFfxbVRugXJiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFWFPMNVIucW6mdXPl[EBxcG:|cHjheIllgWyrbn;zbZRwdCC2dYLuc5ZmeixiS3mgQUAxNjl|IN88UU4> NETJSZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
HEK293 NYDmWndjTnWwY4Tpc44h[XO|YYm= MULJcohq[mm2aX;uJI9nKE2jYjCxNmc2KGKrbnTpcochfG9iQ2jDVlQhTTJ5NVGgcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDPVAhRSByLkmzNFIh|ryPLh?= NV\1b2JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVE{ODVpPkG5OFUyOzB3PD;hQi=>
HEK293 MX\GeY5kfGmxbjDhd5NigQ>? Mn\tTY5pcWKrdHnvckBw\iCPYXKgNVJIPSCkaX7kbY5oKHSxIIfpcIQhfHmyZTDDXGNTPCCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{NCCLQ{mwJF0hOC57N{GxJO69VS5? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
MDA-MB-231 MYPGeY5kfGmxbjDhd5NigQ>? NHH2RmsyOCCvaX7z NH;UNHVFcXOybHHj[Y1mdnRib3[gZolwfGmweXzheIVlKFSQMUSwNFMh\nKxbTDDXGNTPCCFWFPMNVIh[mmwZHnu[{Bld22jaX6gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDwdoVqdmO3YnH0[YQh\m:{IEGwJI1qdnNiZn;scI94\WRiYomgZolwfGmweXzheIVlKFSQMUSwNFMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgN|AhdWmwczD1d4lv\yC|dILldJRifmmmaX6tZ49vcnWpYYTl[EBzcG:mYX3pcoUh[nliZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdIlkKGFuIFXDJF0hOSEQvF2u Mn;uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzN{myNVUoRjJ5MUe5NlE2RC:jPh?=
MDA-MB-231 MnXwSpVv[3Srb36gZZN{[Xl? NFrZdo0yOCCvaX7z Ml\2TY5pcWKrdHnvckBw\iCkaX;0bY56dGG2ZXSgWG4yPDByMzDibY5lcW6pIITvJGNZS1J2IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNicILlbY5kfWKjdHXkJIZweiBzMDDtbY5{KG[xbHzve4VlKGK7IGTONVQxODNiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOzBibXnud{BjgSC{aH;kZY1qdmViZInlMYJie2WmIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzMEBGSyB;IEGg{txONg>? Mn7yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3Mkm1NFAoRjJ7NUK5OVAxRC:jPh?=
MDA-MB-231 MljNSpVv[3Srb36gZZN{[Xl? NHXWNnMyOCCvaX7z MkfaSIl{eGyjY3Xt[Y51KG:oIHLpc5RqdnmuYYTl[E1VVjF2MECzJIZzd21iQ2jDVlQhcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[ox2d3Knc3PlcoNmKHC{ZXnuZ5Vj[XSnZDDmc5IhOTBibXnud{Bnd2yub4fl[EBjgSCkaX;0bY56dGG2ZXStWG4yPDByMzDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB|MDDtbY5{KGK7IIP0doVxfGG4aXTpck1zcG:mYX3pcoUhe3SjaX7pcoch[mG|ZXSgcYlkem:|Y3;wbYMhNCCHQzC9JFEh|ryPLh?= NHTHdYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{mxOFM3OSd-Mke5NVQ{PjF:L3G+
MDA-MB-231 NYDxXJdlTnWwY4Tpc44h[XO|YYm= MlLDNVAhdWmwcx?= MoDpTY5pcWKrdHnvckBw\iCkaX;0bY56dGG2ZXSgWG4yPDByMzDibY5lcW6pIITvJGNZS1J2IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKG[udX;y[ZNk\W6lZTDwdoVqdmO3YnH0[YQh\m:{IEGwJI1qdnNiZn;scI94\WRiYomgZolwfGmweXzheIVlNVSQMUSwNFMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgN|AhdWmwczDifUB{fHKncITheollcW5vcnjv[IFucW6nIIP0ZYlvcW6pIHLhd4VlKGmvbYXuc4ZtNCCHQzC9JFEh|ryPLh?= NWrS[WJVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1NlEzPjFpPkK4OVIyOjZzPD;hQi=>
COS7 NXfldJhbTnWwY4Tpc44h[XO|YYm= NFHpWplFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFchd2ZiaIXtZY4hS1iFUkSgTVI5PEFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IEGuNUDPxE1w M4\TdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
COS7 M4Th[WZ2dmO2aX;uJIF{e2G7 M1rmVWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgTVI5PEFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJIIDyc5RmcW5iR4HpOI16eiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEO[Q1yxNk1qdmS3Y3XkJJBpd3OyaHH0bYR6dGmwb4PpeI9tKHS3cn7veoVzNCCNaTC9JFEvOiEQvF2u MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 NG\sdJdHfW6ldHnvckBie3OjeR?= MYHEbZNxdGGlZX3lcpQhd2ZiW{GyOWleOTKJNTDhcpRq[m:meTDmdo9uKHS{YX7zcYVu[nKjbnWg[I9u[WmwIE[gc4YhcHWvYX6gR3hEWjRiSUK1PWEhdXW2YX70JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|LDDLbUA:KDFwNTFOwG0v NH;XZWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 MWDGeY5kfGmxbjDhd5NigQ>? M1XsV2Rqe3CuYXPlcYVvfCCxZjDbNVI2UV1zMle1JIFvfGmkb3T5JIZzd21idILhcpNu\W2kcnHu[UBld22jaX6gOUBw\iCqdX3hckBEYEOUNDDINlA{SSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOsJGtqKD1iMT64JO69VS5? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 NFzsOZhHfW6ldHnvckBie3OjeR?= M1POPWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgSFE4OU5ibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJINw\XiycnXzd4lv\yCJIIDyc5RmcW5iR4HpOI16eiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEO[Q1yxNk1qdmS3Y3XkJJBpd3OyaHH0bYR6dGmwb4PpeI9tKHS3cn7veoVzNCCNaTC9JFEvQCEQvF2u M1PVOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
COS7 NVX6ZYt7TnWwY4Tpc44h[XO|YYm= NUT6e|R1TGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTD0doFve22nbXLyZY5mKGSxbXHpckA{KG:oIHj1cYFvKEO[Q2K0JGgyOTODIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{whU2liPTCyJO69VS5? Mk[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
U87.CD4 NFO2eodCdnSrdnnyZYwh[XO|YYm= NG\NZXZCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIHPsbY5q[2GuIHnzc4xifGViMUCgbY5n\WO2ZXSgbY4hXTh5LlPEOEBk\WyuczDlfJBz\XO|aX7nJIh2dWGwIFPYR3I1KER{NkLOJI12fGGwdDygTWM2OCB;IEKuOVQyKM7:TT6= MmKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
U87.CD4 M4nyfWFvfGm4aYLhcEBie3OjeR?= NUHFem9DSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTDjcIlvcWOjbDDpd49t[XSnIEGwJIlv\mWldHXkJIlvKFV6Nz7DSFQh[2WubIOg[ZhxemW|c3nu[{BpfW2jbjDDXGNTPCCGMUexUmQzPjKQIH31eIFvfCxiSVO1NEA:KDJwNkm0JO69VS5? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 M13SRWZ2dmO2aX;uJIF{e2G7 M17pcGRqe3CuYXPlcYVvfCCxZjDbNVI2UV1zMle1JIFvfGmkb3T5JIZzd21idILhcpNu\W2kcnHu[UBld22jaX6gOUBw\iCqdX3hckBEYEOUNDDRNlAxXyCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOsJGtqKD1iMj63JO69VS5? NXjLd2pHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
COS7 MXnGeY5kfGmxbjDhd5NigQ>? MX3EbZNxdGGlZX3lcpQhd2ZiW{GyOWleOTKJNTDhcpRq[m:meTDmdo9uKHS{YX7zcYVu[nKjbnWg[I9u[WmwIEWgc4YhcHWvYX6gR3hEWjRiR{KwO2YhdXW2YX70JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|LDDLbUA:KDJwNzFOwG0v MoftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 NGrod2lHfW6ldHnvckBie3OjeR?= NUXHUldRTGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTD0doFve22nbXLyZY5mKGSxbXHpckA2KG:oIHj1cYFvKEO[Q2K0JGczODeZIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{whU2liPTCzJO69VS5? NXLIOJpzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
COS7 NGX1WlRHfW6ldHnvckBie3OjeR?= MUXEbZNxdGGlZX3lcpQhd2ZiW{GyOWleOTKJNTDhcpRq[m:meTDmdo9uKHS{YX7zcYVu[nKjbnWg[I9u[WmwIEegc4YhcHWvYX6gR3hEWjRiVEK4O2EhdXW2YX70JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|LDDLbUA:KDNwMTFOwG0v M3jEVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
COS7 M1S4UmZ2dmO2aX;uJIF{e2G7 NYO3S3dUTGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTD0doFve22nbXLyZY5mKGSxbXHpckA1KG:oIHj1cYFvKEO[Q2K0JGYyPzSDIH31eIFvfCCneIDy[ZN{\WRiaX6gR29UPyClZXzsd{whU2liPTCzMlIh|ryPLh?= NVvPbVU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
COS7 NVroSGhRTnWwY4Tpc44h[XO|YYm= MlWySIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTF{R{WgZY51cWKxZImg[pJwdSCneITyZYNmdGy3bHHyJIxwd3BiMjDv[kBpfW2jbjDDXGNTPCCGMUiyRUBufXSjboSg[ZhxemW|c3XkJIlvKEORU{egZ4VtdHNuIFvpJF0hOy5{IN88UU4> M4[xR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
COS7 Mm\6SpVv[3Srb36gZZN{[Xl? MlvNSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTF{R{WgZY51cWKxZImg[pJwdSC2cnHud41mdWK{YX7lJIRwdWGrbjC2JI9nKGi3bXHuJGNZS1J2IGmyOVZCKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczygT4khRSB2IN88UU4> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 MULGeY5kfGmxbjDhd5NigQ>? NYDaT2FqTGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTDlfJRz[WOnbHz1cIFzKGyxb4CgNkBw\iCqdX3hckBEYEOUNDDONVc3SSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOsJGtqKD1iND6xJO69VS5? NG\LWXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 MWfGeY5kfGmxbjDhd5NigQ>? MlLiSIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTF{R{WgZY51cWKxZImg[pJwdSC2cnHud41mdWK{YX7lJIRwdWGrbjC1JI9nKGi3bXHuJGNZS1J2IG[xPVZCKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczygT4khRSB2Lk[g{txONg>? MnjIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 MnvqSpVv[3Srb36gZZN{[Xl? MkP6SIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTF{R{WgZY51cWKxZImg[pJwdSC2cnHud41mdWK{YX7lJIRwdWGrbjC2JI9nKGi3bXHuJGNZS1J2IFmyOVlYKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczygT4khRSB2Lk[g{txONg>? MkDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 NWDzS4loTnWwY4Tpc44h[XO|YYm= NFvlXGpFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFQhd2ZiaIXtZY4hS1iFUkSgSlE4OkFibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IESuO{DPxE1w NES5Ung9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
COS7 NIH6[YtHfW6ldHnvckBie3OjeR?= NWH5bVgzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFWFPSOEBFOjZ{TjDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[2:neIDy[ZN{cW6pIFegdJJwfGWrbjDHdYk1dXm{IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2jDUFEzNWmwZIXj[YQheGixc4DoZZRq\HmuaX7vd4l1d2xidIXyco93\XJuIFvpJF0hPC55IN88UU4> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 NWLRblNlTnWwY4Tpc44h[XO|YYm= MX;EbZNxdGGlZX3lcpQhd2ZiW{GyOWleOTKJNTDhcpRq[m:meTDmdo9uKHS{YX7zcYVu[nKjbnWg[I9u[WmwIE[gc4YhcHWvYX6gR3hEWjRiU{K2N2EhdXW2YX70JIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|LDDLbUA:KDVwNDFOwG0v NFTuO|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W5PVkyPid-MUe1PVk6OTZ:L3G+
CCRF-CEM MkPsSpVv[3Srb36gZZN{[Xl? NFLwe4I{OCCvaX7z MULJcohq[mm2aX;uJI9nKGGwdHmtR3hEWjRiUFWgZY51cWKxZImgZ4xwdmViMULHOUBjcW6maX7nJJRwKEO[Q2K0JIlvKGi3bXHuJGNEWkZvQ1XNJINmdGy|IIDy[Ylv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDhcpRqNUO[Q2K0JHBGKGGwdHnic4R6KGOub37lJFEzTzViYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOzBibXnud{BjgSCobH;3JIN6fG:vZYTybYMhdWW2aH;kMEBKSzVyIE2gOk4zKM7:TT6= NXzNd4RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNlUzQTVpPkK5NVI2Ojl3PD;hQi=>
COS7 MX\GeY5kfGmxbjDhd5NigQ>? MnvQRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBEYEOUNDDFNlg5SSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZ49mgHC{ZYPzbY5oKEdicILveIVqdiCJcXm0cZlzKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1iFTEGyMYlv\HWlZXSgdIhwe3CqYYTp[JltcW6xc3n0c4whfHW{bn;2[ZItKEurIE2gOk41KM7:TT6= NVnobnhJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
MT4 M{\5UmN6fG:2b4jpZ4l1gSCjc4PhfS=> NY\wbXQ5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVR2IHPlcIx{KGK7IF3UWEBie3OjeTygR2M2OCB;IE[uOUDPxE1w MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN3NkiyO{c,OTl|NU[4Nlc9N2F-
COS7 NYfCUnRRTnWwY4Tpc44h[XO|YYm= NXXo[pVoSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFWFPSOEBCOTd3RjDteZRidnRiZYjwdoV{e2WmIHnuJGNQWzdiY3XscJMh[2:neIDy[ZN{cW6pIFegdJJwfGWrbjDHdYk1dXm{IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2jDUFEzNWmwZIXj[YQheGixc4DoZZRq\HmuaX7vd4l1d2xidIXyco93\XJuIFvpJF0hQC53IN88UU4> MmPyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OUm5NVYoRjF5NUm5PVE3RC:jPh?=
COS7 NFTzfXhHfW6ldHnvckBie3OjeR?= Mn\ISIl{eGyjY3Xt[Y51KG:oIGuxNlVKZTF{R{WgZY51cWKxZImg[pJwdSC2cnHud41mdWK{YX7lJIRwdWGrbjC2JI9nKGi3bXHuJGNZS1J2IGmyOVVCKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczygT4khRSB7Lkeg{txONg>? M4O1dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUm5PVE3Lz5zN{W5PVkyPjxxYU6=
MT-4 MXPGeY5kfGmxbjDhd5NigQ>? NFXCXIxEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgd5lv[3m2aXGg[o9zdWG2aX;uJIJ6KDVyJTDvckBJUVZvMTDpcoZm[3SnZDDNWE01KGOnbHzzMEBGSzVyIE2gNVAh|ryPLh?= MlvpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTl{NUeyPEc,QTl{NUeyPFww[T5?
COS7 M3nES2Z2dmO2aX;uJIF{e2G7 NHzRfYFFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFQhd2ZiaIXtZY4hS1iFUkSgSFE4OU5ibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IEGzJO69VS5? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 M1jybmZ2dmO2aX;uJIF{e2G7 NVr3bZd5TGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTDlfJRz[WOnbHz1cIFzKGyxb4CgNkBw\iCqdX3hckBEYEOUNDDENVg4SSCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOsJGtqKD1iMUSg{txONg>? NXXVfHNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
HL60 NIHKVIFHfW6ldHnvckBie3OjeR?= NGDBbFBFcXOybHHj[Y1mdnRib3[gX|EzPUmfU1TGNYFteGijIH\yc40hS1iFUkSgbY4hcHWvYX6gTGw3OCClZXzsd{whUUN3MDC9JFE2NjJizszNMi=> MmPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzOEiwO|EoRjF7MUi4NFcyRC:jPh?=
COS7 Mkj2SpVv[3Srb36gZZN{[Xl? NXfGbWN3TGm|cHzhZ4Vu\W62IH;mJHsyOjWLXUGyS|Uh[W62aXLv[Jkh\nKxbTDlfJRz[WOnbHz1cIFzKGyxb4CgNkBw\iCqdX3hckBEYEOUNDDBNVc2TiCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOsJGtqKD1iM{[g{txONg>? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
COS7 NVLCTppPTnWwY4Tpc44h[XO|YYm= NHvKVolFcXOybHHj[Y1mdnRib3[gX|EzPUmfMULHOUBidnSrYn;kfUBnem:vIITyZY5{dWWvYoLhcoUh\G:vYXnuJFYhd2ZiaIXtZY4hS1iFUkSgSFI3Ok5ibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzMEBMcSB;IES2JO69VS5? NVXFRYZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PVk6OTZpPkG3OVk6QTF4PD;hQi=>
COS, TZM-bl MnvDSpVv[3Srb36gZZN{[Xl? NH\Ud5hKdmirYnn0bY9vKG:oIFjJWlEhVkx2LUOg[Y53\WyxcHWg[4x6[2:ycn;0[YlvKDF{MD3t[YRq[XSnZDDt[Y1jemGwZTDmeZNqd25iYnX0e4VmdiC4aYL1d{11emGwc3\lZ5Rm\CCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDU3Mh[2WubIOgZY5lKGi3bXHuJHRbVS2kbDDj[YxteyCkeTDseYNq\mW{YYPlMYJie2WmIHPlcIwu[2WubDDmeZNqd25iYYPzZZkhcW5icILld4Vv[2Vib3[gTWM6PTZ2 NWjVb2pqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5OVQ3QDlpPkG3PVU1Pjh7PD;hQi=>
CXCR4+/CD4+/U87 MoTQSpVv[3Srb36gZZN{[Xl? NIHBc|lKdmirYnn0bY9vKG:oIFjJWlEhQTKWSEW5OEBqdm[nY4Tl[EBEYEOUNDuvR2Q1My:XOEegZ4VtdHNidH:gZZN{\XO|IHPvMZJm[2WydH;yJJRzd3Crc32gZZMhdHWlaX\ldoF{\SCjY4Tpeol1gQ>? MoXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMU[2OlMoRjF5MUG2OlY{RC:jPh?=
CXCR4+/CD4+/U87 MkLiSpVv[3Srb36gZZN{[Xl? MXTJcohq[mm2aX;uJI9nKEiLVkGgTHhDOiCrbn\lZ5Rm\CCFWFPSOEswS0R2Kz;VPFch[2WubIOgeI8h[XO|ZYPzJINwNXKnY3XweI9zKHS{b4Dpd40h[XNibIXjbYZmemG|ZTDhZ5Rqfmm2eR?= NIX2Rpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GxOlY3Oyd-MUexNVY3PjN:L3G+
U87 NFzJfGhHfW6ldHnvckBie3OjeR?= NI\PZYMyODByIH7N NVHocYVoSW62YXfvcol{fCCjY4Tpeol1gSCjdDDDXGNTPCCrbjDoeY1idiCXOEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVRF[xMYlv\HWlZXSgcY9lfWyjdHnvckBw\iClQV3QJJBzd2S3Y4Tpc44h[XRiMUCwNEBvVSCkeTDUVk1HWkWWIHHzd4F6 Mm\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NUizOFQoRjF5OUW4N|Q1RC:jPh?=
CHOK1 NGL6bXRHfW6ldHnvckBie3OjeR?= MWTJcoR2[3Srb36gc4YhYzF{NVndUWNROSCmaYPzc4Nq[XSrb36g[pJwdSCFQ2KyM2NZS1J2IHX4dJJme3OnZDDpckBEUE:NMTDj[YxteyCkeTDuc44u\XG3aXzpZpJqfW1iYnnu[Ilv\yCjc4PhfS=> NYOycVM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NVUyOjhpPkG3O|E2OTJ6PD;hQi=>
CHOK1 NFnMRVBHfW6ldHnvckBie3OjeR?= NW\LPXljSW62YXfvcol{fCCjY4Tpeol1gSCjdDDDXGNTPCCneIDy[ZN{\WRiaX6gR2hQUzFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUTEZzLXHsdIhiNWmwZIXj[YQhe2mpbnHsbY5oKGK7IHHldZVwemmwLXLhd4VlKGG|c3H5 M1TpT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{G1NVI5Lz5zN{exOVEzQDxxYU6=
CHOK1 MYfGeY5kfGmxbjDhd5NigQ>? MkjnRY51[WexbnnzeEBi[3Srdnn0fUBifCCFQ2KyM2NZS1J2IHX4dJJme3OnZDDpckBEUE:NMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFOGRkGtZYxxcGFvaX7keYNm\CC|aXfuZYxqdmdiYomgZYVyfW:{aX6tZoF{\WRiYYPzZZk> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdzNUGyPEc,OTd5MUWxNlg9N2F-
CHOK1 M4PrNGZ2dmO2aX;uJIF{e2G7 M1vQVmlv\HWldHnvckBw\iCdMUK1TX1UTEZzYXzwbIEh\Gm|c3;jbYF1cW:wIH\yc40hS1iFUkSg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|IHL5JI5wdi2ncYXpcIljemm3bTDibY5lcW6pIHHzd4F6 M2D5W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{G1NVI5Lz5zN{exOVEzQDxxYU6=
U87.CD4 MVrGeY5kfGmxbjDhd5NigQ>? M{PzPWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS1iFUkSgTFI5OUFibYX0ZY51KGW6cILld5Nm\CCrbjDVPFcvS0R2IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1iFTEGyMYlv\HWlZXSgZ4Ft[2m3bTDtc4JqdGm8YYTpc44> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV7OUmxOkc,OTd3OUm5NVY9N2F-
MT2 M4K2XmFvfGm4aYLhcEBie3OjeR?= NGXZdpIyKHWpL33M M4\1clQh\GG7cx?= M4fQU2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gUXQzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicEK0JIFvfGmpZX6gdJJw\HWldHnvckBifCBzIIXnM41NKGGodHXyJFQh\GG7czDifUBGVEmVQR?= NYXUc3A4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxOlg{OzZpPkKxNVY5OzN4PD;hQi=>
MOLT4 MYrGeY5kfGmxbjDhd5NigQ>? NV3Qell4OTByMDDuUS=> NXjHOoVEUW6qaXLpeIlwdiCxZjDNZYIhOTKJNTDibY5lcW6pIITvJGNZS1J2IHX4dJJme3OnZDDpckBpfW2jbjDNU2xVPCClZXzsd{BifCBzMECwJI5OKGK7IF\BR3Mh[W6jbInzbZM> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3MUOwOUc,OTl2NUGzNFU9N2F-
TZM-bl MlG2RY51cX[rcnHsJIF{e2G7 M3KxVVExOCC3TR?= NI\VNWkzPCCqcoO= MXnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYhVkxvTHHpJIlv\mWldHXkJIlvKGi3bXHuJHRbVS2kbDDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJIlv\mWldHnvckBifCBzMECgeW0hfHKnYYTl[EBj\W[xcnWgeolz[WxiaX7m[YN1cW:wIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIHHzd4F6 NEXtbGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe4N|M4OSd-MkG3PFM{PzF:L3G+
Jurkat MmTKSpVv[3Srb36gZZN{[Xl? M2j3b|AvODFidH:gNVAxKHWP M1fIbmlvcGmkaYTpc44hd2ZiQ2jDVlQhcW5iaIXtZY4hUnW{a3H0JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCKSW[tUoVnNU1zLXnu[JVk\WRibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBl\XCxbHHybZpifGmxbjDheEAxNjBzIITvJFExOCC3TTDifUBLSzFiZInlJIJie2WmIH\seY9z\XOlZX7j[UBl\XCxbHHybZpifGmxbjDhd5NigQ>? NWDSZYFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVE{PjFpPkK2NVkyOzZzPD;hQi=>
MDA-MB-231 MmTlSpVv[3Srb36gZZN{[Xl? NUjrVWo2OTByIH7N M{XTflI1KGi{cx?= M4DpT2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiQ2jDVlQudWWmaXH0[YQh[2inbX;0ZZhqeyCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1iFTEGyMYlv\HWlZXSgZ4VtdCCrbo\hd4lwdiCjdDCxNFAhdk1iYX\0[ZIhOjRiaILzJIJ6KGO{eYP0ZYwhfmmxbHX0JJN1[WmwaX7nMYJie2WmIH3pZ5Jwe2OxcHnjJI1ifHKrZ3XsJIF{e2G7 MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR7NEi0N{c,Ojl2OUS4OFM9N2F-
TZM-bl NWnBW5JSSW62aY\pdoFtKGG|c3H5 M1no[FEhcHJ? MkTsRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSCKWFKyJJB{\XWmb4\pdpV{KGmwZnXjeIVlKGmwIHj1cYFvKFScTT3icEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKGWwdIL5JJRz\WG2ZXSgNUBpeiCyb4P0JIlv\mWldHnvckBu\WG|dYLl[EBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6 M2npXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Mk[2PFQ2Lz5{OEK2Olg1PTxxYU6=
CXCR4+ NHjGcHFHfW6ldHnvckBie3OjeR?= NV20OZJiPiCvZz;r[y=> NIW5VIozKGi{cx?= NGLDZndKdmS3Y4Tpc44hcGWvYYTvdI9q\XSrYzDzeIVuKGOnbHygcY9jcWyrenH0bY9vKGmwIFO1O2JNNzZibX;1d4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gR3hEWjRtIHPlcIx{KGmwIHLsc49lKGG2IE[gcYcwc2duIIPjJI1m[XO3cnXkJIFnfGW{IEKgbJJ{KGK7IFHQR{1kd26sdXfheIVlKGGwdHmtR3hEWjRvc4ThbY5qdmdiYnHz[YQh\myxdzDjfZRwdWW2comgdoVt[XSrdnWgeI8hfmWqaXPs[UBkd262cn;s MkG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|MUS4OFAoRjJ7M{G0PFQxRC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Immunofluorescence
CXCR4; 

PubMed: 28521261     


Representative immunofluorescence images of competition-binding affinity assay of three selected compounds compared to AMD3100. CXCR4 receptors on the cell surface are shown in red fluorescent color in this binding affinity assay using biotinylated TN14003 that binds to CXCR4. When our test compounds are preincubated with the cells and block the binding of biotinylated TN14003, the red fluorescent color gets reduced. The effective concentration (EC) of AMD3100 was 1000 nM, while compounds Ie, IIj and Iq showed much better EC of only 1, 1 and 10 nM, respectively.

β-arrestin2; 

PubMed: 31186083     


Confocal microscopy analysis of CXCR4 and BA2 in Jurkat cells pretreated with vehicle, AMD3100, or X4-2-6 and stimulated with CXCL12, as indicated. Arrows indicate CXCR4 (red), BA2 (green), or colocalization (orange). Images are representative of three independent experiments. Scale bars, 10 μM. DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride.

28521261 31186083
In vivo A single topical application of Plerixafor promotes wound healing in diabetic mice by increasing cytokine production, mobilizing bone marrow EPCs, and enhancing the activity of fibroblasts and monocytes/macrophages, thereby increasing both angiogenesis and vasculogenesis. [3] Cohorts of mice are administered with PBS, IGF1, PDGF, SCF, or VEGF for five consecutive days and Plerixafor on the 5th day. The number and size of the colonies are highest in IGF1 plus Plerixafor injected mice compared to PDGF, SCF and VEGF treated groups, in combination with Plerixafor. [4]

Protocol

Animal Research:[4]
- Collapse
  • Animal Models: Twelve-week-old C57BL/6 mice with segmental bone defect
  • Dosages: 5 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro Ethanol 100 mg/mL (198.89 mM)
Water 3 mg/mL warmed (5.96 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 502.78
Formula

C28H54N8

CAS No. 110078-46-1
Storage powder
in solvent
Synonyms JM 3100
Smiles C1CNCCNCCCN(CCNC1)CC2=CC=C(C=C2)CN3CCCNCCNCCCNCC3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04646603 Not yet recruiting Drug: MRG-001|Drug: Placebo COVID-19 MedRegen LLC|Kendall Healthcare Group Ltd. January 5 2021 Phase 1|Phase 2
NCT03653247 Recruiting Biological: Plerixafor|Drug: Busulfan|Genetic: BIVV003 Sickle Cell Disease Bioverativ a Sanofi company|Sanofi June 28 2019 Phase 1|Phase 2
NCT03547830 Recruiting Drug: Plerixafor|Drug: Gcsf Chronic Granulomatous Disease Federal Research Institute of Pediatric Hematology Oncology and Immunology April 13 2019 Phase 2
NCT03442673 Recruiting Drug: Vinorelbine|Drug: Gemcitabine|Drug: G-CSF Multiple Myeloma University Hospital Inselspital Berne September 17 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    How about the half-life of the product (Cat S8030)?

  • Answer:

    The biological half-life for this drug is 3-5 hours: https://en.wikipedia.org/wiki/Plerixafor.

CXCR Signaling Pathway Map

Related CXCR Products

Tags: buy Plerixafor (AMD3100) | Plerixafor (AMD3100) ic50 | Plerixafor (AMD3100) price | Plerixafor (AMD3100) cost | Plerixafor (AMD3100) solubility dmso | Plerixafor (AMD3100) purchase | Plerixafor (AMD3100) manufacturer | Plerixafor (AMD3100) research buy | Plerixafor (AMD3100) order | Plerixafor (AMD3100) mouse | Plerixafor (AMD3100) chemical structure | Plerixafor (AMD3100) mw | Plerixafor (AMD3100) molecular weight | Plerixafor (AMD3100) datasheet | Plerixafor (AMD3100) supplier | Plerixafor (AMD3100) in vitro | Plerixafor (AMD3100) cell line | Plerixafor (AMD3100) concentration | Plerixafor (AMD3100) nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID