Medroxyprogesterone

For research use only.

Catalog No.S3635 Synonyms: MP

Medroxyprogesterone Chemical Structure

Molecular Weight(MW): 344.49

Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.

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Biological Activity

Description Medroxyprogesterone (MP) is a synthetic pregnane steroid and a derivative of progesterone. It is a potent progesterone receptor agonist.
In vitro

Medroxyprogesterone acetate (MPA, 10 nM) treatment for 48 h induces proliferation of the cells (1.6-fold induction). MPA induces cyclin D1 expression (3.3-fold induction). MPA increases both the protein level (2.2-fold induction) and promoter activity (2.7-fold induction) of cyclin D1 in MCF-7 cells transfected with PRB but not with PRA. Although MPA transcriptionally activates cyclin D1 expression, cyclin D1 promoter does not have progesterone-responsive element-related sequence. MPA induces the transient phosphorylation of Akt (2.7-fold induction at 5 min) and also phosphorylation of inhibitor of NFkappaBalpha (IkappaBalpha) (2.3-fold induction)[1]. MPA activates glucocorticoid receptors, which could mimic part of the anti-atherosclerotic effects of glucocorticoids, and has, on the other hand, also anti-androgenic activity, which might diminish protective oestrogen effects[2].

In vivo Long-term treatment with MPA and MPA + E2 increases arterial thrombosis despite inhibitory effects of MPA on atherosclerosis in ApoE-deficient mice. Increased thrombin formation, reduced smooth muscle content and remodelling of non-collagenous plaque matrix may be involved in the pro-thrombotic effects. Thus, MPA exhibits differential effects on arterial thrombosis and on atherosclerosis. In monkeys, MPA interferes with anti-atherosclerotic oestrogen effects whereas progesterone does not[2].

Protocol

Cell Research:[1]
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  • Cell lines: T47D human breast cancer cells
  • Concentrations: 10 nM
  • Incubation Time: 4 days
  • Method: The cells are plated at a density of 10 or 40 × 104 cells/well in 12-well plates and allowed to attach overnight. The cells are then growth arrested by incubation in phenol red-free DMEM with 10% charcoal-stripped serum for 24 h followed by treatment with vehicle or MPA by exchanging the culture medium containing these agents with fresh medium every 24 h for 4 d. A Neubauer chamber is used to count cells, and trypan blue experiments are carried out in quadruplicate.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 68 mg/mL (197.39 mM)
Ethanol 14 mg/mL (40.63 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 344.49
Formula

C22H32O3

CAS No. 520-85-4
Storage powder
in solvent
Synonyms MP
Smiles CC1CC2C(CCC3(C)C2CCC3(O)C(C)=O)C4(C)CCC(=O)C=C14

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04334278 Not yet recruiting Behavioral: 3RP-OA|Behavioral: Health Enhancement Program Knee Osteoarthritis|Obesity|Depression University of Kentucky|Massachusetts General Hospital|Brigham and Women''s Hospital January 2021 Not Applicable
NCT04078139 Not yet recruiting Drug: The MP/RP group|Drug: The RP group Pain Postoperative Beijing Tiantan Hospital October 1 2020 Phase 4
NCT03636165 Not yet recruiting Drug: Methylprednisolone|Drug: Ropivacaine Pain Postoperative|Post-Craniotomy Headache Beijing Tiantan Hospital September 2020 Phase 4
NCT04282798 Not yet recruiting Other: Personalized Music Intervention Alzheimer Disease Johns Hopkins University June 2020 Not Applicable
NCT04147052 Not yet recruiting Behavioral: iSLEEPms Multiple Sclerosis Johns Hopkins University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) June 1 2020 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID