Catalog No.S7958

Lificiguat(YC-1) Chemical Structure

Molecular Weight(MW): 304.34

YC-1 is an nitric oxide (NO)-independent activator of soluble guanylyl cyclase(sGC) and an inhibitor of Hypoxia-inducible factor-1alpha (HIF-1alpha).

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Biological Activity

Description YC-1 is an nitric oxide (NO)-independent activator of soluble guanylyl cyclase(sGC) and an inhibitor of Hypoxia-inducible factor-1alpha (HIF-1alpha).
sGC [1] HIF-1α [2]
In vitro

YC-1 is an allosteric activator of soluble guanylyl cyclase (sGC). YC-1 increases the catalytic rate of the enzyme and sensitizes the enzyme toward its gaseous activators nitric oxide or carbon monoxide. YC-1 alone activates the enzyme only 10-fold, but it potentiates the CO- and NO-dependent activation of sGC, resulting in stimulation of the highly purified enzyme that may be several hundred- to several thousand-fold [1]. It inhibits platelet aggregation and vascular contraction and also inhibits HIF-1 activity in vitro. YC-1 completely blocks HIF-1α expression at the post-transcriptional level and consequently inhibits the transcription factor activity of HIF-1 in hepatoma cells cultured under hypoxic conditions, suggesting that these effects of YC-1 are likely to be linked with the oxygen-sensing pathway and not with the activation of soluble guanylyl cyclase[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A498 cells NX7UUGNuS3m2b4TvfIlkcXS7IHHzd4F6 M{fYV|Q5KGh? Mn\xR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ6QCClZXzsd{BqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MD6zJO69VQ>? NFfmTIMzPjJ|NUm1NS=>
ACHN cells M1G3XGZ2dmO2aX;uJIF{e2G7 NXP1fHpGPDhiaB?= M13JUWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFHDTG4h[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeR?= NH;NdGMzODB7N{S1Oi=>
HeLa cells NHPOTJVHfW6ldHnvckBie3OjeR?= MYDJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZzYXzwbIEhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIlvKGi3bXHuJGhmVGFiY3XscJMh\XiycnXzd4lv\yCKUlWtUJVkKGGodHXyJFEzKGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OS53IN88US=> M4fZVlI1QTByNk[y
NCI-H226 cells M2r1c2Z2dmO2aX;uJIF{e2G7 MYC0PEBp NFfyPVhCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDJ{NjDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvQSEQvF2= NYPBSmF1OjByOUe0OVY>
AGS cells MUfGeY5kfGmxbjDhd5NigQ>? MnXaTY5pcWKrdHnvckBw\iCqeYDvfIliKGmwZIXj[YQhUEmIMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gbJVu[W5iQVfTJINmdGy|IHL5JINmdGxvYnHz[YQhUFKHIILldI9zfGW{IHHzd4F6NCCLQ{WwQVIh|ryP NGfCNnMyPzN{OEWzNi=>
HepG2 cells Mnq1R5l1d3SxeHnjbZR6KGG|c3H5 MmewO|IhcA>? M4DXWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9Nk4zKM7:TR?= MUmyOFkxODZ4Mh?=
HCT116 cells NXO1T3lxS3m2b4TvfIlkcXS7IHHzd4F6 M2HlWVczKGh? NHnnU4FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zNjJizszN NIfORoMzPDlyME[2Ni=>
HEK293 cells M2TqSGZ2dmO2aX;uJIF{e2G7 M3e2elE3KGh? MVXJcohq[mm2aX;uJI9nKGi7cH;4bYEucW6mdXPl[EBJUUZzYXzwbIEhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5JIlvKEiHS{K5N{Bk\WyuczDpcoN2[mG2ZXSg[o9zKDF4IHjyd{BjgSCqeYDvfIliKHKnc4DvcpNmKGWuZX3lcpQu\HKrdnXuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF06NjJzIN88US=> NUP1WYlEOTl2M{W2OlE>
Hep3B cells MV3GeY5kfGmxbjDhd5NigQ>? MWXJcohq[mm2aX;uJI9nKGi7cH;4bYEhcW6mdXPl[EBJUUZzLXHsdIhiKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBqdiCqdX3hckBJ\XB|QjDj[YxteyCkeTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUGzMlgh|ryP M3qyflE4QDh2NEm1
U251HRE cells M2\UT2Z2dmO2aX;uJIF{e2G7 NFfTWGpKdmirYnn0bY9vKG:oIHj5dI95cWFvaX7keYNm\CCKSV[xJIFkfGm4YYTpc44hcW5iaIXtZY4hXTJ3MVjSSUBk\WyuczDifUBk\WyuIHLhd4VlKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OTRwODFOwG0> NX3xNIVnOTh3MEG2NFE>
HL60 cells MmPlR5l1d3SxeHnjbZR6KGG|c3H5 NIDuZ5I1QCCq NFLCeGJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlUvOjdizszN MortNlM5OzF6MEm=
HT1080 cells MVfGeY5kfGmxbjDhd5NigQ>? MkiyTY5pcWKrdHnvckBw\iCKSV[xJIlvKGi3bXHuJGhVOTB6MDDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKDW6SGLFM5BIVDNxVlXHSk9GOWJicnXwc5J1\XJicHzhd41q\CCycnWtbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KGmwY4XiZZRqd25idX7k[ZIhcHmyb4jpZUBkd26maYTpc45{KG[xcjCyOEBpenNiYomgTHJGNWS{aY\lckBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:PDhwNDFOwG0> MWSyOVc4OzBzNB?=

... Click to View More Cell Line Experimental Data

In vivo The administration of YC-1 to experimental animals results in the inhibition of the platelet-rich thrombosis and a decrease of the mean arterial pressure, which correlates with increased cGMP levels [1]. YC-1 effectively inhibits tumor growth in tumor-bearing mice. The inhibition of HIF-1 activity in tumors from YC-1-treated mice is associated with blocked angiogenesis and an inhibition of tumor growth, while the anti-platelet aggregation effect of YC-1 does not appear to affect tumor growt[2].


Cell Research:[2]
+ Expand
  • Cell lines: Hep3B cells
  • Concentrations: 0.01-10 μM
  • Incubation Time: 24 h
  • Method: Hep3B cells are plated in a six-well plate at a density of 1 × 105 cells/well in α-modified Eagle medium supplemented with 10% heat-inactivated FBS and incubated overnight. Cells are treated with YC-1 (0.01-10 μM) or vehicle (DMSO) for 5 minutes and are then subjected to normoxia or hypoxia for 24 hours. VEGF levels in the conditioned media are quantified by using the Quantikine human VEGF Immunoassay kit. The VEGF concentrations are quantified by comparison with a series of VEGF standard samples included in the assay kit.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: Male nude (BALB/cAnNCrj–nu/nu) mice
  • Formulation: DMSO
  • Dosages: 30 µg/g
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 60 mg/mL (197.14 mM)
Ethanol 60 mg/mL (197.14 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 304.34


CAS No. 170632-47-0
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID