Molecular Weight(MW): 570.38
KC7F2 is a selective HIF-1α translation inhibitor with IC50 of 20 μM in a cell-based assay.
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|Description||KC7F2 is a selective HIF-1α translation inhibitor with IC50 of 20 μM in a cell-based assay.|
KC7F2 inhibits HIF-1α protein synthesis but not its mRNA transcription. KC7F2 inhibits HRE-driven transcription and decreases HIF-1α protein levels in LN229-HRE-AP cells. KC7F2 shows a dose-response cytotoxicity with IC50 of approximately 15 to 25 μM in cancer cells MCF7, LNZ308, A549, U251MG, and LN229. In D54MG glioma cells, KC7F2 inhibits colony formation, especially under hypoxia.  In hypoxic microglial cultures, KC7F2 downregulates the expression of TfR and DMT, and reduces the HIF-1α mediated iron accumulation. 
|In vivo||KC7F2 significantly reduces the latent period in the pentylenetetrazole kindling rat model and increases the rate of spontaneous recurrent seizures during the chronic stage in the lithium-pilocarpine rat model. |
HIF transcriptional activity assay:Cells are incubated at 37癈 in a humidified atmosphere containing 5% CO2 and 21% O2 (normoxia) or 1% O2 (hypoxia) in a hypoxia workstation. The LN229-HRE-AP reporter cell line for HIF transcriptional activity is created by stably transfecting LN229 cells with the pACN188 plasmid, which contains an alkaline phosphatase gene driven by six HREs derived from the VEGF gene.
|In vitro||DMSO||100 mg/mL (175.32 mM)|
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