CU-CPT9a

For research use only.

Catalog No.S9730

CU-CPT9a Chemical Structure

CAS No. 2165340-32-7

CU-CPT9a is a potent and specific antagonist of Toll-like receptor 8 (TLR8) with IC50 of 0.5 nM.

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Biological Activity

Description

CU-CPT9a is a potent and specific antagonist of Toll-like receptor 8 (TLR8) with IC50 of 0.5 nM.

Targets
TLR8 [1]
(Cell-free assay)
0.5 nM
In vitro

PBMC treatment with R848 or RNA-40 induces IL-6 production, and this activation is suppressed by CU-CPT9a in a dose-dependent manner. A dose-dependent suppression of IL-6 production by CU-CPT9a is also observed when cells are stimulated with violacein. Previous addition of CU-CPT9a only blocks IL-1β stimulated by R848 and has no effect on violacein-induced IL-1β stimulation.[2]

Protocol

Cell Research:

[2]

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  • Cell lines: Human PBMCs
  • Concentrations: 0.02 μmol/L, 0.2 μmol/L, 2 μmol/L, 20 μmol/L
  • Incubation Time: 1 h
  • Method:

    Human PBMCs are cultured (50 μL of 6 × 106 live cells/mL) in a 96-well plate. Cells are treated with different concentrations of CU-CPT9a (0.02, 0.2, 2 and 20 μmol/L) or medium as a negative control and are incubated for 1 h at 37 °C, 5% CO2. After this, cells are treated with violacein (30 and 15 μmol/L), R848 (1 μmol/L), LPS (50 ng/mL) or RNA-40 (5 μg/mL). After incubating for 20 h, the culture supernatants are collected and used to measure the concentration of IL-6 or IL-1β by ELISA according to the manufacturer’s instructions.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 53 mg/mL (199.76 mM)
Water Insoluble
Ethanol '''''''10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 265.31
Formula

C17H15NO2

CAS No. 2165340-32-7
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID