Home Immunology & Inflammation TLR antagonist CU-CPT9a For research use only.

CU-CPT9a

CU-CPT9a is a potent and specific antagonist of Toll-like receptor 8 (TLR8) with IC50 of 0.5 nM.

CU-CPT9a Chemical Structure

CU-CPT9a Chemical Structure

CAS: 2165340-32-7

Purity & Quality Control

Batch: S973001 DMSO] 53 mg/mL] false] Ethanol] 10 mg/mL] false] Water] Insoluble] false Purity: 99.93%
99.93

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Biological Activity

Description

CU-CPT9a is a potent and specific antagonist of Toll-like receptor 8 (TLR8) with IC50 of 0.5 nM.
Targets
TLR8 [1]
(Cell-free assay)
0.5 nM
In vitro
In vitro

PBMC treatment with R848 or RNA-40 induces IL-6 production, and this activation is suppressed by CU-CPT9a in a dose-dependent manner. A dose-dependent suppression of IL-6 production by CU-CPT9a is also observed when cells are stimulated with violacein. Previous addition of CU-CPT9a only blocks IL-1β stimulated by R848 and has no effect on violacein-induced IL-1β stimulation.[2]
Cell Research Cell lines Human PBMCs
Concentrations 0.02 μmol/L, 0.2 μmol/L, 2 μmol/L, 20 μmol/L
Incubation Time 1 h
Method

Human PBMCs are cultured (50 μL of 6 × 106 live cells/mL) in a 96-well plate. Cells are treated with different concentrations of CU-CPT9a (0.02, 0.2, 2 and 20 μmol/L) or medium as a negative control and are incubated for 1 h at 37 °C, 5% CO2. After this, cells are treated with violacein (30 and 15 μmol/L), R848 (1 μmol/L), LPS (50 ng/mL) or RNA-40 (5 μg/mL). After incubating for 20 h, the culture supernatants are collected and used to measure the concentration of IL-6 or IL-1β by ELISA according to the manufacturer’s instructions.

Chemical Information & Solubility

Molecular Weight 265.31 Formula

C17H15NO2
CAS No. 2165340-32-7 SDF --
Smiles COC1=CC2=C(C=C1)C(=CC=N2)C3=CC(=C(O)C=C3)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 53 mg/mL ( (199.76 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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