PD98059

For research use only.

Catalog No.S1177

386 publications

PD98059 Chemical Structure

CAS No. 167869-21-8

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

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Selleck's PD98059 has been cited by 386 publications

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Choose Selective MEK Inhibitors

Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  MkTPSpVv[3Srb36gRZN{[Xl? NVGyNpg{OjEEoN88US=> NX\xepY3OSCq MWPicI9kc3NiRGTDSE1qdmS3Y3XkJGRTPSCneIDy[ZN{cW:w MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ7Nki2Nkc,OjN4OU[4OlI9N2F-
AML 1# NX\4SGc2SXCxcITvd4l{KEG|c3H5 Ml\4NlDDqM7:TR?= MkflNVIhcA>? MUHlcohidmOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGOx M3j5PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
KG-1  NF;RbodCeG:ydH;zbZMhSXO|YYm= MWCyNOKh|ryP NXHuN|dPOTJiaB?= NFm1UWJmdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHMy NIraZVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewOlY6OSd-MkO3NFY3QTF:L3G+
HUVECs MXLBdI9xfG:|aYOgRZN{[Xl? MnywNk81KM7:TR?= NFjxTZYzPC92ODDo MUDpcoR2[2W|IHPlcIwh\GWjdHi= NYqxN4lNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc2OjBpPkKzO|A4PTJyPD;hQi=>
HepG2  MWjGeY5kfGmxbjDBd5NigQ>? NFOxd2wzOMLizszN NHSyeGszPCCq M{H6XIlvcGmkaYTzJJRp\SCKTz2xJJBzd3SnaX6g[ZhxemW|c3nvckBkdy22cnXheIVlKHerdHigcYV1\m:{bXnu Mk\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe2NFkoRjJ|N{C3OlA6RC:jPh?=
NB4  NYXHPI9CSXCxcITvd4l{KEG|c3H5 M4rRVlExNzJyL{[wJO69VQ>? NXTUWVc2OS53IHi= MXzEUXNQ Mofa[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2ZXSge4l1cCCSYXPsbZRigGWu NXPuWG5mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|U2PDFpPkKzO|M2PTRzPD;hQi=>
BxPC-3 cells NWLG[oJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\TN45{OjEEoN88US=> NInpTlgxNjViaB?= NYq2ZWlbcW6qaXLpeJMhXkWJRj3BMZJm\3WuYYTl[EBJXV[HQzDndo94fGhiYX7kJJR2[mViZn;ycYF1cW:wIHnu[JVk\WRiYomgVGFTNTJiQWC= Mn7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NkSwOFYoRjJ|N{[0NFQ3RC:jPh?=
MKN45 NWPXZnlTSXCxcITvd4l{KEG|c3H5 MoPWNVAh|ryPwrC= NHu2NJozPCCq MYXpcoNz\WG|ZYOgeIhmKESDUGStbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 NHzaSFNCeG:ydH;zbZMhSXO|YYm= M{CyZlExKM7:TdMg NVfnTIFjOjRiaB?= NFfP[5BqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
MKN45 MnzsSpVv[3Srb36gRZN{[Xl? NXftfHVxOTBizszNxsA> NVHadoJoOjRiaB?= M2T4WolvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIFXST|EwOg>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 MmjhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\FfWoyOCEQvF5CpC=> MXqyOE81QC95MjDo MkfmbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[29vdILlZZRm\CC5aYToJGRCWFR? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
MKN45 NFjEOHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4njOFExKM7:TdMg MYCyOE81QC95MjDo MoXGbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[29vdILlZZRm\CC5aYToJGRCWFR? M33sVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
SGC7901 M3nFeGZ2dmO2aX;uJGF{e2G7 MmG1NVAh|ryPwrC= Mnq2NlQhcA>? MYfpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwbI9{eGixconsZZRm\CCHUluxM|I> M3zzPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
HUASMCs NF7FbpBHfW6ldHnvckBCe3OjeR?= NULJOlJJOTBizszNxsA> NE[4dIozPCCq NUnTfWJx\GmvaX7pd4hmeyCDbnegTWku[2G3c3XkJHNQS1N|IH3SUmEh[W6mIIDyc5RmcW5iZYjwdoV{e2mxbtMg NETjWnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ixOlQ3QCd-MkO4NVY1Pjh:L3G+
HUASMCs NFn5blJHfW6ldHnvckBCe3OjeR?= M17wTVExKM7:TdMg NETROpYzPCCq M2X5[IlvcGmkaYTzJGFv\yCLST3pcoR2[2WmIFXST|EwOiCyaH;zdIhwenmuYYTpc44hdGW4ZXy= M4TpSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG2OFY5Lz5{M{ixOlQ3QDxxYU6=
EPOR/CR3 NF24[nRHfW6ldHnvckBCe3OjeR?= NIrTSoo2OMLizszN MoWzN{Bp MmLvdoVlfWOnczDFVG8h[W6mL3;yJGlNNTNvaX7keYNm\CC2aHWgeJlzd3OrbnWgdIhwe3Cqb4L5cIF1cW:wwrC= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEezNUc,OjN6MkC3N|E9N2F-
NB4  NVTRW5E3TnWwY4Tpc44hSXO|YYm= MkTTNVAh|ryPwrC= MWm3NkBp M1T5eGROW09? NI\uOXVqdmirYnn0d{Bl[XOjdHnubYIucW6mdXPl[EBETDFzYjDlfJBz\XO|aX;u NIPRS|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOVU5PSd-MkO4NlU2QDV:L3G+
HL60  MnjYSpVv[3Srb36gRZN{[Xl? NYDMb5BTOjEEoN88US=> NWfPfZp7PzJiaB?= NGjXWYtFVVOR NGD1TYtqdmirYnn0d{Bl[XOjdHnubYIucW6mdXPl[EBETDFzYjDlfJBz\XO|aX;u NIXOWoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOVU5PSd-MkO4NlU2QDV:L3G+
HUVECs MmflSpVv[3Srb36gRZN{[Xl? MonnNlUh|ryP MVmxJIg> NF\LbG9qdmO{ZXHz[UBPTi4QulKgdFY2KG63Y3zlZZIhfHKjboPsc4NifGmxbh?= MoD1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEGwNFgoRjJ|OUCxNFA5RC:jPh?=
LNCaP  NGTMfIxHfW6ldHnvckBCe3OjeR?= M3\hV|ExKM7:TdMg MnT0NUBp M123WYRm[3KnYYPld{B1cGViRVfGJJVxemWpdXzheIVlKHBvWVKtNS=> NFXIPFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{izPFMyQCd-MkO4N|g{OTh:L3G+
HL-60 M321fGFxd3C2b4Ppd{BCe3OjeR?= MYe1NOKh|ryP Ml\BNUBp MkOzdoV{[3WnczDCRVE1PSCvZXTpZZRm\CCjcH;weI9{cXN? NEHxbHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0PFc2OSd-MkO5OFg4PTF:L3G+
HepG2 NXfucIoxTnWwY4Tpc44hSXO|YYm= M2noflQxKM7:TR?= M4PyXVYwOTJiaB?= NV7nS2UxcW6qaXLpeJMhfGinIHnuZ5Jm[XOnIH;mJJAuTVKNMTDhcoQheC2lLVr1ckBxem:2ZXnuJIV5eHKnc4Ppc44h[nliUFy= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl2Mki1NUc,OjN7NEK4OVE9N2F-
HPAF-II MnXFSpVv[3Srb36gRZN{[Xl? NHLLbmIyOCEQvF5CpC=> NUH4c241PiCq MULpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= Mk\aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O3NVAoRjJ|OUezO|ExRC:jPh?=
BxPC-3 MljNSpVv[3Srb36gRZN{[Xl? MmPINVAh|ryPwrC= NF7uWGs3KGh? M2TyOIlv[3KnYYPlJI1qWi1zNEOg[ZhxemW|c3nvci=> M3q5fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
COLO205 NFTRfYxCeG:ydH;zbZMhSXO|YYm= M{e3flExNzJyL{SwJO69VQ>? NYLzdVk{OjRiaB?= MUfpcoR2[2W|IFTORUBt[WSmZYKg[o9zdWG2aX;u NVX0T3dWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkyODhpPkK0NFE6OTB6PD;hQi=>
G292  NVK2Tog3SXCxcITvd4l{KEG|c3H5 NETNd2w{OMLizszN Mnm5NkBp NFfmb4tz\XO2b4Lld{Bk[XC|YXnjbY4ucW6mdXPl[EBk\WyuIHTlZZRp M3rTdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEGyPVMxLz5{NECxNlk{ODxxYU6=
SGC7901 MVnBdI9xfG:|aYOgRZN{[Xl? MkLDNlDDqM7:TR?= MlHsNUBp M{XDPYlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= MkjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMke3OVAoRjJ2MEK3O|UxRC:jPh?=
MGC803  M17teGFxd3C2b4Ppd{BCe3OjeR?= MXiyNOKh|ryP Mn6wNUBp NYLPV5QzcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliSV\OMe6yyqCjbnSgOgKBui2GRmXS NU[1NHN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlc4PTBpPkK0NFI4PzVyPD;hQi=>
HPMC MlH6RZBweHSxc3nzJGF{e2G7 NHnINZEyOMLizszN MlfGNlTDqGh? MY\y[ZZmenOnczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JGhIWESV NITLdoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC0Nlg{QCd-MkSwOFI5Ozh:L3G+
HCT-8 NXz1S3RjSXCxcITvd4l{KEG|c3H5 NXTCfWVQOTEEoN88US=> MWGyOOKhcA>? NE\SdY1l\WO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUA2NU[X NHS3NpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVg3Oyd-MkSwPVU5PjN:L3G+
HPMC NGDQNnJHfW6ldHnvckBCe3OjeR?= MkDGNVDDqM7:TR?= M4HmUlQ5KGh? M{\Zb5JmfmW{c3XzJJRp\SClaHHu[4V{KGmwIHPlcIwhdW:{cHjvcI9ogSCrbnT1Z4VlKGK7IFjHVGRU M4XJRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
A549 MYfGeY5kfGmxbjDBd5NigQ>? MVO1NOKh|ryP NH74Z44zKGh? Mlf0Zoxw[2u|IFXST{BxcG:|cHjvdplt[XSrb36gcYVlcWG2ZXSgZpkhOSx{LV7R NFnDWmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2O|czPyd-MkSwOlc4Ojd:L3G+
Caco-2  M4Tzc2Fxd3C2b4Ppd{BCe3OjeR?= M{PXdlExyqEQvF2= M12xfVI1yqCq NHTuc4Fl\WO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUA2NU[X M{HjOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1PFY{Lz5{NEC5OVg3OzxxYU6=
AGS NYLaXnZ5TnWwY4Tpc44hSXO|YYm= NI\nb5gyOCEQvF5CpC=> MWSwMlUhcA>? NGGzeZRqdmirYnn0d{B1cGVidYDy[Yd2dGG2aX;uJI9nKHSqZTDJUE05KGenbnW= NETXd3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwOlE3Pid-MkSxNFYyPjZ:L3G+
Ca9-22 NF;KT49HfW6ldHnvckBCe3OjeR?= MlTPN{DPxE1? MVexM|IhcA>? NHzLPGNz\WS3Y3XzJGhjWi2rbnT1Z4VlKEGWRj2yJJBpd3OyaH;yfYxifGmxbh?= M37qSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK2OVMzLz5{NEGyOlU{OjxxYU6=
Ca9-22 MVLGeY5kfGmxbjDBd5NigQ>? M{T3R|Mh|ryP NYn3OWpEOSCq NH7WSYFi[m:uaYPo[ZMhfGinIHHibYxqfHlib3[gTIJTKHSxIHnu[JVk\SCLTD24JJBzd2S3Y4Tpc44> NYjNXmZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY2OzJpPkK0NVI3PTN{PD;hQi=>
HAECs M{fJeGZ2dmO2aX;uJGF{e2G7 M3GzVFExKM7:TR?= NXzYXYF2OSCq NXTCVII1yqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|NE[1O{c,OjRzM{S2OVc9N2F-
Caco-2 MXfGeY5kfGmxbjDBd5NigQ>? NYTkVIVIPTEEoN88US=> NGTZR|E1QGh? NEfvOHNFVVOR Mmn4[Y5p[W6lZYOgeIhmKG2UTlGgcIV3\Wy|IH;mxsBUS06QMVGsSnh[TDNuwrDMR3QtyqCOT2isxsBJUUZ|QT|CpHpIOTZuwrDQSGU3ScLiYX7kxsBNT0GOU{G2xsBo\W6nc9MgZ48ufHKnYYTl[EB4cXSqIFTlfC=> NGrIPIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2NVY6PSd-MkSxOlE3QTV:L3G+
MCF-7 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjlTG0yOCEQvF2= MXy0PIg> MULEUXNQ NGnTNYlz\X[ncoPld{BDVkZvaX7keYNm\CClZXzsJIN6[2ynIHHydoV{fA>? NYfNZoRbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlM1ODRpPkK0NVY{PDB2PD;hQi=>
SMMC7721 NXTmOItCTnWwY4Tpc44hSXO|YYm= Mlv2NlUwPTBizszN MUeyOEBp MYLzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJJAuSWu2IH;yJJAuTVKNMT:yxsA> NFziSlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2PFA2Pid-MkSxOlgxPTZ:L3G+
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DLD-1  MlHNSpVv[3Srb36gRZN{[Xl? NFn4VFYzOMLizszN NGfLdmI1QCCq NFe1SHBz\WS3Y3XzJJRp\SCETlnQN{BmgHC{ZYPzbY9vKHC{ZT30doVifGWmIIfpeIghPS2jenGt[GM> M4\HOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxOVgyLz5{NEKxNVU5OTxxYU6=
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7402 NXfvdXZlSXCxcITvd4l{KEG|c3H5 NHzG[2o{OCEQvF2= NY\C[m5EPSCm NWLtOINw\GWlcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdg>? MkLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGyOVMoRjJ2MkGxNlU{RC:jPh?=
MCF-7 MVXGeY5kfGmxbjDBd5NigQ>? M4fNWVIxKM7:TR?= MYWxJIg> MnjxZYJwdGm|aHXzJIV5eHKnc4Ppc44hd2ZicHjvd5Bpd3K7bHH0[YQhTVKNIHPvMZRz\WG2bXXueEB4cXSqIHPvcop2\2G2ZR?= M1jKXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkG2Nlg6Lz5{NEKxOlI5QTxxYU6=
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ARPE-19 M4XzbWZ2dmO2aX;uJGF{e2G7 MoLoNlAh|ryP M2nZS|AvPSCq M3SwOYlvcGmkaYTzJGFx\Wyrbj3pcoR2[2WmIIDoc5NxcG:{eXzheIlwdiCxZjDFdosh[W6mIFHreC=> NFnKbm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyO|kyQCd-MkSyNlc6OTh:L3G+
MG-63 MYnGeY5kfGmxbjDBd5NigQ>? NIXEdJUzOCEQvF2= NV\aR3c5OC53IHi= MX3icI9kc3NidHjlJGNJNWmwZIXj[YQheGixc4Doc5J6dGG2ZXSgSWxMOSCycn;0[YlvKGW6cILld5Nqd25? NHXxNGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKzPVY1OCd-MkSyN|k3PDB:L3G+
SGC-7901  MnK4RZBweHSxc3nzJGF{e2G7 NF7ieHMzOCEQvF2= MonaNlQhcA>? M2HyPIlvcGmkaYTzJGNRNW2nZHnheIVlKGGyb4D0c5Nqew>? NFLUbHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0NVM2OSd-MkSyOFE{PTF:L3G+
NUGC2 NFLXRW5HfW6ldHnvckBCe3OjeR?= Mo[1OVAh|ryP NUC0eFNpPDhiaB?= MmfXSG1UVw>? NGXNelVqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZjDIUGEuSTB{IH;yJGhNSS2DMkSgcY9t\WO3bHXz NVrhNXJVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
NUGC3 NYizS4c2TnWwY4Tpc44hSXO|YYm= NWTU[mhMPTBizszN NX7C[Hg5PDhiaB?= MVXEUXNQ NWrHVmlUcW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> NYnNV3RGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
KATOIII  MXLGeY5kfGmxbjDBd5NigQ>? MYG1NEDPxE1? NU\2bGFiPDhiaB?= MV\EUXNQ M3TMfIlv[3KnYYPld{BmgHC{ZYPzbY9vKG:oIFjMRU1CODJib4KgTGxCNUF{NDDtc4xm[3WuZYO= NUnSW2NpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
NCI-N87  NXfuXZVmTnWwY4Tpc44hSXO|YYm= NXrv[G1WPTBizszN NHPNfHM1QCCq NHLocZhFVVOR MYrpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
OE19 NWPFbY01TnWwY4Tpc44hSXO|YYm= MVy1NEDPxE1? NYLORlhsPDhiaB?= M{LEZmROW09? NG\RWIVqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZjDIUGEuSTB{IH;yJGhNSS2DMkSgcY9t\WO3bHXz NFHGO5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
MKN7 MYfGeY5kfGmxbjDBd5NigQ>? NGewfI02OCEQvF2= MUS0PEBp NFjtdlFFVVOR MXnpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| MlHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
TE4 NFj5RlZHfW6ldHnvckBCe3OjeR?= MYC1NEDPxE1? NXPI[3Q6PDhiaB?= M4fTcGROW09? MV;1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBJVEFiY3zhd5MhUQ>? NYTDSG5TRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
KYSE30 MVTGeY5kfGmxbjDBd5NigQ>? MUC1NEDPxE1? MkLkOFghcA>? M{\n[2ROW09? NY[1SXNbfXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFzBJINt[XO|IFm= M1XYeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
TE5 NESwT5dHfW6ldHnvckBCe3OjeR?= NFv6cGQ2OCEQvF2= M4\IOFQ5KGh? NEnUU|JFVVOR NVr1SVFpfXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFzBJINt[XO|IFm= NV;UZ4lTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE1 M2[0S2Z2dmO2aX;uJGF{e2G7 MX[1NEDPxE1? MYS0PEBp NUmxRnE5TE2VTx?= M2POW5VxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKEiOQTDjcIF{eyCL MnjqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
TE3 NIH5ZYxHfW6ldHnvckBCe3OjeR?= NX3DSolLPTBizszN M17QOVQ5KGh? MVzEUXNQ M3;BfpVxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKEiOQTDjcIF{eyCL NEHYSJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
KYSE30 MXTGeY5kfGmxbjDBd5NigQ>? NYTkb296OjBxNUCvNVAxKM7:TR?= Mn:zOFghcA>? M1LoTWROW09? NVjNZppEcW6qaXLpeJMheC2HcnugZY5lKHexcoTtZY5vcW5iZH;3cpJm\3WuYYTl[EBxNUGtdDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NH30R2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
TE1 MljlSpVv[3Srb36gRZN{[Xl? MlK0NlAwPTBxMUCwJO69VQ>? NXTQ[IpiPDhiaB?= M1TaXGROW09? MVTpcohq[mm2czDwMWVzcyCjbnSge49zfG2jbn7pckBld3ewcnXneYxifGWmIICtRYt1KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NV\WSmhtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE4 NWrDW5ZCTnWwY4Tpc44hSXO|YYm= NVfmfppiOjBxNUCvNVAxKM7:TR?= MoDzOFghcA>? M3G3SGROW09? MYfpcohq[mm2czDwMWVzcyCjbnSge49zfG2jbn7pckBld3ewcnXneYxifGWmIICtRYt1KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M2r2dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
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HepG2 NEXDWXpCeG:ydH;zbZMhSXO|YYm= Mnv6NlAh|ryP Mln3NlQhcA>? MYjpcohq[mm2czDFVmsyNzJicHjvd5Bpd3K7bHH0bY9vKGGwZDDlcohidmOnczDWRlEucW6mdXPl[EBieG:ydH;zbZM> Mlv3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NEe5NFkoRjJ2MkS3PVA6RC:jPh?=
HepG2 MVrGeY5kfGmxbjDBd5NigQ>? M1v0UFIxKM7:TR?= NYrxcWFHOiCq NXfOPHFm\W6qYX7j[ZMhXkJzLXnu[JVk\WRiRl;YU|NiKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fS=> NF\kTnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0O|kxQSd-MkSyOFc6ODl:L3G+
MC-3 NVzTZ3NySXCxcITvd4l{KEG|c3H5 M4HHb|ExKM7:TR?= M1LvVVI1KGh? MYHwc5RmdnSrYYTl[EBOTVOFLXnu[JVk\WRiYYDvdJRwe2m|IHnuJEBk\Wyucx?= M3PkclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkewOVI{Lz5{NEK3NFUzOzxxYU6=
Raji  NU\YT41xTnWwY4Tpc44hSXO|YYm= NFXXXlMyOCEQvF2= M134PFEhcA>? NHznc3FjdG:la4OgbJNDSU[IIHnu[JVk\WRiRYLrNU8zKHCqb4PwbI9zgWyjdHnvci=> NYC4NVk{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3OzBpPkK0NlY6PjNyPD;hQi=>
Raji  NYL0NnVZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3:yRlExKM7:TR?= MY[xJIg> Mo\rbY5pcWKrdIOgeIhmKGKjc3HsJI9zKGi|QlHGSk1{fGmvdXzheIVlKGOnbHygdJJwdGmoZYLheIlwdiCjbnSgeoli[mmuaYT5 NVXqOoV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3OzBpPkK0NlY6PjNyPD;hQi=>
A549 MVfGeY5kfGmxbjDBd5NigQ>? NVK1WGgyOzBizszN MVqwMlUhcA>? MW\EUXNQ NYnCXlQ5cW6qaXLpeJMhfGi{b33ibY4ucW6mdXPl[EBEN0WEUN8yJHRpejJ|NdMgdIhwe3Cqb4L5cIF1cW:w MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5N{[5Okc,OjR{N{e2PVY9N2F-
HCSMCs M3HDemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvscZEyOTBizszN MoH0NlQhcA>? MVLicI9kc3NiRlHCVFQucW6mdXPl[EBJS0GVTVOgdJJwdGmoZYLheIlwdg>? NHT5WoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOxNlM5OSd-MkSzNVI{QDF:L3G+
PANC-1 MX\GeY5kfGmxbjDBd5NigQ>? MWqyNEDPxE1? M{PaZ|Q5KGh? MmGzbY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[g{rQ3TCCrbjDy[ZNxd26|ZTD0c{B1cGViUGDBVu61yqCjZ3;ubZN1yqB? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7NEGzN{c,OjR{OUSxN|M9N2F-
A549 NGXiW4tHfW6ldHnvckBCe3OjeR?= NY[x[nAzOzBizszN MWewMlUhcA>? MlfCSG1UVw>? M1fhcIlvcGmkaYTzJJRp\SC2aILvcYJqdi2rbnT1Z4VlKEmOLUivR3hEVDhvTIXjJIFkfGm4aYT5 NVPhS5pORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|c3QTZpPkK0Nlc4Pjl4PD;hQi=>
SW480 MYrGeY5kfGmxbjDBd5NigQ>? NVK1d|kxOTBizszN MnryNlAhcA>? NVHhepl{TE2VTx?= M3TEdpN2eHC{ZYPz[ZMhfGinIFPSWEBi[3Srdnn0fS=> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{NEO2Okc,OjR|MkSzOlY9N2F-
HEK 293 MUHGeY5kfGmxbjDBd5NigQ>? Ml\DNVAh|ryP M4HPXlUhcA>? M4DDSGROW09? M37Pe4lvcGmkaYTzJHdvfC2rbnT1Z4VlKM7{LXPheIVvcW5xVFPGOEBi[3Srdnn0fUBidmRiboXjcIVieiEQsj3jZZRmdmmwIHHjZ5VufWyjdHnvci=> NXmwenF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlQ{PjZpPkK0N|I1OzZ4PD;hQi=>
HEK 293 MX;GeY5kfGmxbjDBd5NigQ>? NXPUd5R5OTBizszN MoTCOUBp MVvEUXNQ NYrsSZJ1e3WycILld5NmeyC2aHWgR3JVKGGldHn2bZR6 NES3c|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyOFM3Pid-MkSzNlQ{PjZ:L3G+
HL-60  NFXWeFlHfW6ldHnvckBCe3OjeR?= NWTQUGNUOTBxMkCg{txO MXexJIg> NXXGfpJucW6qaXLpeJMhfGinwrDOMkBkcGmwZX7zbZNmgHS{YXP0xsBqdmS3Y3XkJIRq\m[ncnXueIlifGmxbjDpcpRwKGe{YX71cI9kgXSncx?= NEG0SWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1O|AzOCd-MkSzOVcxOjB:L3G+
HL-60 MkHFSpVv[3Srb36gRZN{[Xl? NWHlV2V5OiEEtV2= NYfaUoU6OTZiaB?= NV;Le|duTE2VTx?= MlTabY5pcWKrdIOgeIhmKGG|c3;jbYF1cW:wIH;mJJBUPjJzIGLh[k0yKGGwZDDOSmFV[zNuIHHu[EB1cGViUlGtbY5lfWOnZDDwbI9{eGixconsZZRqd25ib3[gcpVkdGWjcjDOSmFV[zN? MkHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|M{CwOlgoRjJ2M{OwNFY5RC:jPh?=
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A375 NHfXPFdE\WyuIFnueoF{cW:wIFHzd4F6 MUWxNQKBmzJyINM1US=> M2HkblI1KGh? MWHy[YR2[2W|IH3lcIFvd22jIHPlcIwhcW64YYPpc44> NVv5PJdHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlYxOzZpPkK0OFY3ODN4PD;hQi=>
HBMEC NF\UZoFHfW6ldHnvckBCe3OjeR?= MW[xNEDPxE1? M2fIUVEhcA>? MknVZoxw[2u|IG\FS2YucW6mdXPl[EBGeGiDMjDlfJBz\XO|aX;u NXLFNItzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVg6QDJpPkK0OFU5QTh{PD;hQi=>
HCT-15 MYHGeY5kfGmxbjDBd5NigQ>? NXi1[3hYOSCq MlLGZZR1\W63YYTld{BRT0V{LXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb:Kh NXzp[nVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|E1OjVpPkK1OFMyPDJ3PD;hQi=>
HCT-15 NXjZSpBTSXCxcITvd4l{KEG|c3H5 MljMNUBp MmjvZYJwdGm|aHXzJJRp\SCycn;0[YN1cX[nIHXm[oVkfHNib3[gVGdGOsLiYXfhbY5{fCCldYLjeY1qdi2rbnT1Z4VlKGGyb4D0c5Nqew>? M1vvS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOxOFI2Lz5{NUSzNVQzPTxxYU6=
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HepG2  MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonjNlAh|ryP NXjyd4JFOjRiaB?= M2PZVJN2eHC{ZYPz[ZMhXEeILd8yNU1qdmS3Y3XkJINmdGxicILvcIln\XKjdHnvckBidmRiaX72ZZNqd25? Mn7wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NkC0PFgoRjJ3NU[wOFg5RC:jPh?=
SW480 NUC4OFlKTnWwY4Tpc44hSXO|YYm= NInFUGszOMLizszN M1X3PFHDqGh? NFjVSpZz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mJGFVTjNicILveIVqdg>? MnjsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NEe4NVYoRjJ3NES3PFE3RC:jPh?=
MDA-MB-231 NX;l[ZkyTnWwY4Tpc44hSXO|YYm= NH7QUVkzPSEQvF2= Mn\TNk0{KGh? Mmnv[IVkemWjc3XzJJAuTVKNMT:yJIFv\CCVMUCwRVQh\XiycnXzd4lwdg>? M33vXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW1PFc2Lz5{NUW1OVg4PTxxYU6=
HepG2  M3v0O2Z2dmO2aX;uJGF{e2G7 MlLGNVAh|ryP MnLmOUBp MnfXZoxw[2u|IIDoc5NxcG:{eXzheIVlKE2DUFvzJIlv\HWlZXSgZpkh\XixZ3Xuc5V{KFSJRj5OtlE> NIrBS2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NFQ5QCd-MkW1OlA1QDh:L3G+
MCF-7  M4SwNWZ2dmO2aX;uJGF{e2G7 MlfPNVAh|ryP MnHENUBp NYf3bolGcW6qaXLpeJMhUUxvMUit[Y5p[W6lZXSgZ4VtdCCvaXfyZZRqd25? NYHEOXNKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3NlcxOTFpPkK1O|I4ODFzPD;hQi=>
H1355 NIXxfolHfW6ldHnvckBCe3OjeR?= MV:yOUDPxE1? MoDvNUBp NXrlT4Vz[my3boTzJJRp\SCEW3HdVE1qdmS3Y3XkJIlv[3KnYYPlJIlvKHCqb4PwbI8uS2itMTDhcoQheGixc4Doc{1GWktiZYjwdoV{e2mxbh?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4OUG4NUc,OjV5NkmxPFE9N2F-
PC12 M4S4PGZ2dmO2aX;uJIF{e2G7 NYi2NIg{UW6qaXLpeIlwdiCxZjDu[ZJ3\SCpcn;3eIgh\mGldH;yMY1m\GmjdHXkJG1CWCCtaX7hd4Uh[WO2aY\peJkhcW5iaIXtZY4hWENzMjDj[YxteyxiSVO1NEA:KDJizszNMi=> M3XwSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEe3N|Y{Lz5zOEC3O|M3OzxxYU6=
HT-29 NFvIVHhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NH\XXVY2KGSjeYO= MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDViZHH5d{BjgSCZU2SxJIF{e2G7LDDJR|UxKD1iNDFOwG0v NVTFR|dpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwO|g{OTZpPkK1NFc5OzF4PD;hQi=>
IEC6 NV7X[lRGTnWwY4Tpc44h[XO|YYm= NYfOOmRZPSCvaX7z NIjpTldKdmirYnn0bY9vKG:oIF3FT|EwOiCrbjDyZZQhUUWFNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hTVKNMT:yJIxwd3BicHjvd5Bpd3K7bHH0bY9vKGSxc3XkJFMxKG2rboOgZoVnd3KnIITvJJN1cW23bHH0bY9vKHerdHigNVAmKHOncoXtJIZweiB3IH3pcpMh[nliaX3teY5w[myxdITpcochdWW2aH;kMEBKSzVyIE2gOE4zKM7:TT6= Mn3uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyN{izNVYoRjJ3MEe4N|E3RC:jPh?=
NG 108-15 NEDkNlFHfW6ldHnvckBie3OjeR?= M4\5fmNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZYJwdGm|aDDNRXBMKGGldHn2bZR6KGmwIH3veZNmKG6ndYLvZoxie3SxbXGgZY5lKHKjdDDncIlwdWFiaInidollKE6JIEGwPE0yPSClZXzsd{whSWO2aY\peJkhRSBzMDFOwG0v M1HGXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUO3N|U1Lz5zNUWzO|M2PDxxYU6=
PC12 MWrGeY5kfGmxbjDhd5NigQ>? M3K4UFExKHWP MXu1JIhzew>? MkPyRYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCMTlugbY5pcWKrdH;yJHNRPjByMUK1JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2NU[4OUc,OjF|NEW2PFU9N2F-
PC12 M1rUemZ2dmO2aX;uJIF{e2G7 MYqxNEB2VQ>? MYO1JIhzew>? MmfKRYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCPRVuyJIlvcGmkaYTvdkBWODF{NjDmc5IhOSCqcjDi[YZwemViY3;tdI92dmRiYXTkbZRqd25iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M361S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{S1Olg2Lz5{MUO0OVY5PTxxYU6=
PC12 MWDGeY5kfGmxbjDhd5NigQ>? M17WO|ExKHWP NHezUoU2KGi{cx?= M3vydGFkfGm4YYTpc44hd2ZiToLmNk9CWkViaX6gdoF1KFCFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgTG8uOSCycn;0[YlvKGmwZIXjeIlwdiCjdDCxNEB2VSCjZoTldkA2KGi{czDwdoV1emWjdHXkJJdqfGhiTVXLNUBqdmirYnn0c5IhWER7OEC1PUBnd3JiMTDodkBj\W[xcnWgZ49ueG:3bnSgZYRlcXSrb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MnX5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
HeLa NVrQRpp1TnWwY4Tpc44h[XO|YYm= MWqyNEB2VQ>? NW[wOmNEOyCqcoO= MoPyTY5pcWKrdHnvckBw\iCHUluxM|IheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMkCgeW0h[W[2ZYKgN{BpenNiYomgW4V{fGW{bjDicI91fGmwZzDhcoFtgXOrcx?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5ME[xOUc,OjN3N{C2NVU9N2F-
RD MXfxTHRUKGG|c3H5 Mlz5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ M2TMfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MVnxTHRUKGG|c3H5 NXv3XIJGeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> MkjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD M4DaSZFJXFNiYYPzZZk> MXTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucx?= NHuzUHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NUjrZpZLeUiWUzDhd5NigQ>? MV3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NYPsWW5xRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 MkL0dWhVWyCjc4PhfS=> M13wXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NHy2VI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MDA-MB-231 NUX5TYRuTnWwY4Tpc44h[XO|YYm= MkDKOVAhfU1? NGW0SlRFd3ewcnXneYxifGmxbjDv[kBOVVB{IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M1u3cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUK2NlI3Lz5{MkmyOlIzPjxxYU6=
MDA-MB-231 MVHGeY5kfGmxbjDhd5NigQ>? NFjhTWw2OCC3TR?= MWfEc5dvemWpdXzheIlwdiCxZjDNUXA6KGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NW\3eIk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlYzOjZpPkKyPVI3OjJ4PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol

Kinase Assay:

[1]

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In vitro MEK-inhibitory activity:

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.
Cell Research:

[1]

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  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.


    (Only for Reference)
Animal Research:

[7]

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  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (52.37 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to formulate this inhibitor for i.p. injection?

  • Answer:

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID