PD98059

Catalog No.S1177

For research use only.

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

PD98059 Chemical Structure

CAS No. 167869-21-8

Selleck's PD98059 has been cited by 423 publications

Purity & Quality Control

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Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  MUHGeY5kfGmxbjDBd5NigQ>? NHvsN5AzOMLizszN MnrmNUBp NHPVSVZjdG:la4OgSHRETC2rbnT1Z4VlKESUNTDlfJBz\XO|aX;u NEDNOVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[5Olg3Oid-MkO2PVY5PjJ:L3G+
AML 1# MojTRZBweHSxc3nzJGF{e2G7 MW[yNOKh|ryP NY[5b2ZjOTJiaB?= NIWxe4NmdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHMy M3fveVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
KG-1  NYi3WpZ7SXCxcITvd4l{KEG|c3H5 NIDZW2IzOMLizszN MV2xNkBp NFvVN21mdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHMy M2fCRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
HUVECs M1PyU2Fxd3C2b4Ppd{BCe3OjeR?= Mn;oNk81KM7:TR?= M1S2PFI1NzR6IHi= NYPSepUzcW6mdXPld{Bk\WyuIHTlZZRp MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{WyNEc,OjN5MEe1NlA9N2F-
HepG2  MVzGeY5kfGmxbjDBd5NigQ>? NWe0PIU{OjEEoN88US=> MUCyOEBp M{Dy[YlvcGmkaYTzJJRp\SCKTz2xJJBzd3SnaX6g[ZhxemW|c3nvckBkdy22cnXheIVlKHerdHigcYV1\m:{bXnu Mm[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe2NFkoRjJ|N{C3OlA6RC:jPh?=
NB4  NX3jOlFISXCxcITvd4l{KEG|c3H5 MmXxNVAwOjBxNkCg{txO M2\wOlEvPSCq NXm3dI1yTE2VTx?= MkXE[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2ZXSge4l1cCCSYXPsbZRigGWu MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NUW0NUc,OjN5M{W1OFE9N2F-
BxPC-3 cells MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXyxOYIyOjEEoN88US=> NWPGeXhWOC53IHi= NWCydHVKcW6qaXLpeJMhXkWJRj3BMZJm\3WuYYTl[EBJXV[HQzDndo94fGhiYX7kJJR2[mViZn;ycYF1cW:wIHnu[JVk\WRiYomgVGFTNTJiQWC= MkPHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NkSwOFYoRjJ|N{[0NFQ3RC:jPh?=
MKN45 MmrhRZBweHSxc3nzJGF{e2G7 NFjJNHQyOCEQvF5CpC=> NGX1T2ozPCCq M{i3T4lv[3KnYYPld{B1cGViRFHQWE1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| NEXQcZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
SGC7901 MYDBdI9xfG:|aYOgRZN{[Xl? NGfOXGEyOCEQvF5CpC=> NILZR3czPCCq NHzUZmtqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= M{W3V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
MKN45 NUjwSWhmTnWwY4Tpc44hSXO|YYm= M163WFExKM7:TdMg MYGyOEBp MU\pcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwbI9{eGixconsZZRm\CCHUluxM|I> NI\kN2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
SGC7901 M375OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLmN4wyOTBizszNxsA> M4DLVVI1NzR6L{eyJIg> M3rGXYlvcGmkaYTzJINmdGxiZ4Lve5RpKGOxLYTy[YF1\WRid3n0bEBFSVCW MlG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 NXjvZ29jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUWxNEDPxE4EoB?= M4PaSlI1NzR6L{eyJIg> Mn3CbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[29vdILlZZRm\CC5aYToJGRCWFR? NIDsRpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
SGC7901 NFfzWotHfW6ldHnvckBCe3OjeR?= M{TKOlExKM7:TdMg MkflNlQhcA>? MknHbY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[gdIhwe3Cqb4L5cIF1\WRiRWLLNU8z M1PDNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
HUASMCs M2nnfWZ2dmO2aX;uJGF{e2G7 Mm[zNVAh|ryPwrC= NHX4ZXkzPCCq NELHW5plcW2rbnnzbIV{KEGwZzDJTU1k[XW|ZXSgV29EWzNibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:wwrC= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzNkS2PEc,OjN6MU[0Olg9N2F-
HUASMCs M{H1bmZ2dmO2aX;uJGF{e2G7 Mm\ENVAh|ryPwrC= NV7DbGlQOjRiaB?= NXOxb4xbcW6qaXLpeJMhSW6pIFnJMYlv\HWlZXSgSXJMOS9{IIDoc5NxcG:{eXzheIlwdiCuZY\lcC=> NYHSVZB{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NVY1PjhpPkKzPFE3PDZ6PD;hQi=>
EPOR/CR3 M{LyT2Z2dmO2aX;uJGF{e2G7 M{LYVFUxyqEQvF2= M{XET|MhcA>? NUL6SZdZemWmdXPld{BGWE9iYX7kM49zKEmOLUOtbY5lfWOnZDD0bIUhfHm{b4PpcoUheGixc4Doc5J6dGG2aX;uxsA> Mlr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkC3N|EoRjJ|OEKwO|MyRC:jPh?=
NB4  M1mzT2Z2dmO2aX;uJGF{e2G7 MYWxNEDPxE4EoB?= MU[3NkBp NYHCNldSTE2VTx?= NHTh[llqdmirYnn0d{Bl[XOjdHnubYIucW6mdXPl[EBETDFzYjDlfJBz\XO|aX;u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NUW4OUc,OjN6MkW1PFU9N2F-
HL60  NFXYN5NHfW6ldHnvckBCe3OjeR?= NGPVTmMzOMLizszN NH3oSpI4OiCq M4nTSGROW09? M4n4SolvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= M124fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK1OVg2Lz5{M{iyOVU5PTxxYU6=
HUVECs Mk\oSpVv[3Srb36gRZN{[Xl? MYSyOUDPxE1? NIDFXFAyKGh? MVrpcoNz\WG|ZTDOSk3PwkJicE[1JI52[2ynYYKgeJJidnOub3PheIlwdg>? MljFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEGwNFgoRjJ|OUCxNFA5RC:jPh?=
LNCaP  MV;GeY5kfGmxbjDBd5NigQ>? NXWxN|g2OTBizszNxsA> MkGzNUBp NEn3dYtl\WO{ZXHz[ZMhfGinIFXHSkB2eHKnZ4XsZZRm\CCyLWnCMVE> MnHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6M{izNVgoRjJ|OEO4N|E5RC:jPh?=
HL-60 NUP6[VNVSXCxcITvd4l{KEG|c3H5 NXTGXHBwPTEEoN88US=> M3q5dVEhcA>? MX;y[ZNkfWW|IFLBNVQ2KG2nZHnheIVlKGGyb4D0c5Nqew>? NX;LeoNmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFg4PTFpPkKzPVQ5PzVzPD;hQi=>
HepG2 NIT5[YdHfW6ldHnvckBCe3OjeR?= M3PXWFQxKM7:TR?= MUi2M|EzKGh? MV;pcohq[mm2czD0bIUhcW6lcnXhd4Uhd2ZicD3FVmsyKGGwZDDwMYMuUnWwIIDyc5RmcW5iZYjwdoV{e2mxbjDifUBRVA>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl2Mki1NUc,OjN7NEK4OVE9N2F-
HPAF-II NWC0dpNyTnWwY4Tpc44hSXO|YYm= MkHCNVAh|ryPwrC= MXm2JIg> MWLpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= Mmm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O3NVAoRjJ|OUezO|ExRC:jPh?=
BxPC-3 MonMSpVv[3Srb36gRZN{[Xl? MmX0NVAh|ryPwrC= MoW0OkBp M{eyR4lv[3KnYYPlJI1qWi1zNEOg[ZhxemW|c3nvci=> NELuXmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m3N|cyOCd-MkO5O|M4OTB:L3G+
COLO205 NGS0OmpCeG:ydH;zbZMhSXO|YYm= NIHxb|EyOC9{MD:0NEDPxE1? NETmV2QzPCCq MXjpcoR2[2W|IFTORUBt[WSmZYKg[o9zdWG2aX;u MljpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUmxNFgoRjJ2MEG5NVA5RC:jPh?=
G292  Ml\hRZBweHSxc3nzJGF{e2G7 NVTsfndpOzEEoN88US=> MX:yJIg> NGHFWVdz\XO2b4Lld{Bk[XC|YXnjbY4ucW6mdXPl[EBk\WyuIHTlZZRp MnHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUK5N|AoRjJ2MEGyPVMxRC:jPh?=
SGC7901 M4\sWmFxd3C2b4Ppd{BCe3OjeR?= MVqyNOKh|ryP MXyxJIg> MXjpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBKTk5vzsJCpIFv\CB34pEyMWRHXVJ? MnXyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMke3OVAoRjJ2MEK3O|UxRC:jPh?=
MGC803  NV\mXWRiSXCxcITvd4l{KEG|c3H5 NE\oToozOMLizszN NYn6RWxGOSCq M4n6WIlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= MmPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMke3OVAoRjJ2MEK3O|UxRC:jPh?=
HPMC NEi3[XNCeG:ydH;zbZMhSXO|YYm= MXyxNOKh|ryP M1v3SFI1yqCq Ml;wdoV3\XK|ZYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBJT1CGUx?= M4S3RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
HCT-8 NIjsWppCeG:ydH;zbZMhSXO|YYm= MoHVNVDDqM7:TR?= MmrENlTDqGh? NXHYU3kx\GWlcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhPS2IVR?= NEe2VoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVg3Oyd-MkSwPVU5PjN:L3G+
HPMC NXLCWnNrTnWwY4Tpc44hSXO|YYm= M1XQfFExyqEQvF2= MVO0PEBp Mn7sdoV3\XK|ZYOgeIhmKGOqYX7n[ZMhcW5iY3XscEBud3KyaH;sc4d6KGmwZIXj[YQh[nliSFfQSHM> M1HxSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
A549 NFHBfplHfW6ldHnvckBCe3OjeR?= MlnDOVDDqM7:TR?= M3jFTVIhcA>? NWqx[XNx[myxY3vzJGVTUyCyaH;zdIhwenmuYYTpc44hdWWmaXH0[YQh[nliMTyyMW5S NIjL[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2O|czPyd-MkSwOlc4Ojd:L3G+
Caco-2  NUjrdo1CSXCxcITvd4l{KEG|c3H5 NEjOPFkyOMLizszN MVeyOOKhcA>? M2q1eYRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUi2N{c,OjRyOUW4OlM9N2F-
AGS Mlf2SpVv[3Srb36gRZN{[Xl? NI\5V2QyOCEQvF5CpC=> M2DVSlAvPSCq MorlbY5pcWKrdIOgeIhmKHWycnXneYxifGmxbjDv[kB1cGViSVytPEBo\W6n M2n2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUC2NVY3Lz5{NEGwOlE3PjxxYU6=
Ca9-22 MXzGeY5kfGmxbjDBd5NigQ>? MofxN{DPxE1? M2r2flEwOiCq M{fIPJJm\HWlZYOgTIJTNWmwZIXj[YQhSVSILUKgdIhwe3Cqb4L5cIF1cW:w Mk\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[1N|IoRjJ2MUK2OVMzRC:jPh?=
Ca9-22 M3PFWWZ2dmO2aX;uJGF{e2G7 NGi3O5U{KM7:TR?= MkjBNUBp MWDhZo9tcXOqZYOgeIhmKGGkaXzpeJkhd2ZiSHLSJJRwKGmwZIXj[UBKVC16IIDyc4R2[3Srb36= NFTBNZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOlU{Oid-MkSxNlY2OzJ:L3G+
HAECs M4f5XWZ2dmO2aX;uJGF{e2G7 MVyxNEDPxE1? Mkj0NUBp NX;IeFFiyqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= NX\Tfo1KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|Q3PTdpPkK0NVM1PjV5PD;hQi=>
Caco-2 M{DPWmZ2dmO2aX;uJGF{e2G7 MVe1NOKh|ryP M{LGNFQ5cA>? NXjm[oE3TE2VTx?= NUTSd4Zn\W6qYX7j[ZMhfGinIH3SUmEhdGW4ZXzzJI9nyqCVQ17ONWEtTli\REOsxsBNS1RuwrDMU3gtyqCKSV[zRUzDqFqJMU[sxsBRTEV4QdMgZY5lyqCOR1HMV|E3yqCpZX7ld:Kh[29vdILlZZRm\CC5aYToJGRmgA>? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4MU[5OUc,OjRzNkG2PVU9N2F-
MCF-7 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PjTFExKM7:TR?= NFHERoY1QGh? MVLEUXNQ NV;NUJlZemW4ZYLz[ZMhSk6ILXnu[JVk\WRiY3XscEBkgWOuZTDhdpJme3R? M4fwfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[zOFA1Lz5{NEG2N|QxPDxxYU6=
SMMC7721 NFfLTZZHfW6ldHnvckBCe3OjeR?= M4DxWFI2NzVyIN88US=> M3zhflI1KGh? NV3KT3l6e3WycILld5NmeyC2aHWg[ZhxemW|c3nvckBw\iCyLVHreEBweiCyLVXST|EwOsLi M1;4ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[4NFU3Lz5{NEG2PFA2PjxxYU6=
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MCF-7 NHHMeW5HfW6ldHnvckBCe3OjeR?= M1OyVFExyqEQvF2= NFr2NpoyOC9|MDDtbY4> MULy[YR2[2W|IITo[UBWXFBvZHXw[Y5l\W62IFXST{BxcG:|cHjvdplt[XSrb36= NHT5U3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO5NFgyQSd-MkSzPVA5OTl:L3G+
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PC3  M3LTfmFxd3C2b4Ppd{BCe3OjeR?= MVq1NEDPxE1? NHWye4IxNjViaB?= MXnpcohq[mm2czDNTHkuPDR7LXnu[JVk\WRiYYDvdJRwe2m|wrC= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{NEi4PUc,OjR2MkS4PFk9N2F-
HPAEpiCs  M{nBdWZ2dmO2aX;uJGF{e2G7 MYqzNEDPxE1? NETu[ZIyKGh? M4KzcYlvcGmkaYTzJHRPTi4QsTDzeIlufWyjdHXkJJA1Oi:yNESgUWFRUyCyaH;zdIhwenmuYYTpc44> M2HycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NESxPFcxLz5{NES0NVg4ODxxYU6=
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A375 NVnmVlk3S2WubDDJcpZie2mxbjDBd5NigQ>? NUfOZZJWOTEkgKOyNEDDvU1? NXy0OG02OjRiaB?= NFuySJpz\WS3Y3XzJI1mdGGwb33hJINmdGxiaX72ZZNqd25? NYD3NGh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlYxOzZpPkK0OFY3ODN4PD;hQi=>
HBMEC MXHGeY5kfGmxbjDBd5NigQ>? MX6xNEDPxE1? NWDyb5VUOSCq NY\qcpE3[myxY3vzJHZGT0ZvaX7keYNm\CCHcHjBNkBmgHC{ZYPzbY9v MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3OEm4Nkc,OjR2NUi5PFI9N2F-
HCT-15 MX\GeY5kfGmxbjDBd5NigQ>? NGr4bZYyKGh? MYrheJRmdnWjdHXzJHBITTJvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiRYLrxsA> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|MUSyOUc,OjV2M{G0NlU9N2F-
HCT-15 M2H5W2Fxd3C2b4Ppd{BCe3OjeR?= MVGxJIg> Mmj1ZYJwdGm|aHXzJJRp\SCycn;0[YN1cX[nIHXm[oVkfHNib3[gVGdGOsLiYXfhbY5{fCCldYLjeY1qdi2rbnT1Z4VlKGGyb4D0c5Nqew>? NFP1VGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzNVQzPSd-MkW0N|E1OjV:L3G+
786-O NWDCb3pMSXCxcITvd4l{KEG|c3H5 M4qzb|UxyqEQvF2= NYfBTYhlOjRiaB?= Mn21dI91\W62aXH0[ZPDqHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeJMhd2ZiTlO= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVyOES3Okc,OjR3MEi0O|Y9N2F-
HepG2  Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDTbpIzOCEQvF2= NIPKeHEzPCCq NHTiboh{fXCycnXzd4V{KFSJRj5OtlEucW6mdXPl[EBk\WyuIIDyc4xq\mW{YYTpc44h[W6mIHnueoF{cW:w NVi1c|lkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OlA1QDhpPkK1OVYxPDh6PD;hQi=>
SW480 M1S4OmZ2dmO2aX;uJGF{e2G7 MUOyNOKh|ryP M4f2dVHDqGh? NFjtV|hz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mJGFVTjNicILveIVqdg>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2N{ixOkc,OjV2NEe4NVY9N2F-
MDA-MB-231 MoPxSpVv[3Srb36gRZN{[Xl? MofKNlUh|ryP MVKyMVMhcA>? NHP5eFJl\WO{ZXHz[ZMheC2HUluxM|Ih[W6mIGOxNFBCPCCneIDy[ZN{cW:w M4jXZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW1PFc2Lz5{NUW1OVg4PTxxYU6=
HepG2  NIDvelRHfW6ldHnvckBCe3OjeR?= MV6xNEDPxE1? NWS4OY1JPSCq M3K1foJtd2OtczDwbI9{eGixconsZZRm\CCPQWDLd{BqdmS3Y3XkJIJ6KGW6b3flco92eyCWR1[t{tIy NYXzTItRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OlA1QDhpPkK1OVYxPDh6PD;hQi=>
MCF-7  MY\GeY5kfGmxbjDBd5NigQ>? M1m4TVExKM7:TR?= MYqxJIg> M1fqXolvcGmkaYTzJGlNNTF6LXXubIFv[2WmIHPlcIwhdWmpcnH0bY9v M130TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{K3NFEyLz5{NUeyO|AyOTxxYU6=
H1355 NUXUSG84TnWwY4Tpc44hSXO|YYm= MWeyOUDPxE1? M2TZTVEhcA>? M{ThfoJtfW62czD0bIUhSlujXWCtbY5lfWOnZDDpcoNz\WG|ZTDpckBxcG:|cHjvMWNpczFiYX7kJJBpd3OyaH:tSXJMKGW6cILld5Nqd25? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4OUG4NUc,OjV5NkmxPFE9N2F-
PC12 NHu0TFRHfW6ldHnvckBie3OjeR?= NGfRd|dKdmirYnn0bY9vKG:oIH7ldpZmKGe{b4f0bEBn[WO2b4KtcYVlcWG2ZXSgUWFRKGurbnHz[UBi[3Srdnn0fUBqdiCqdX3hckBRSzF{IHPlcIx{NCCLQ{WwJF0hOiEQvF2u NInCdFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC3O|M3Oyd-MUiwO|c{PjN:L3G+
HT-29 NELpXYZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MX:1JIRigXN? MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDViZHH5d{BjgSCZU2SxJIF{e2G7LDDJR|UxKD1iNDFOwG0v NVvUXIU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwO|g{OTZpPkK1NFc5OzF4PD;hQi=>
IEC6 M4jhTWZ2dmO2aX;uJIF{e2G7 NWn5cWJzPSCvaX7z NH;ub3RKdmirYnn0bY9vKG:oIF3FT|EwOiCrbjDyZZQhUUWFNjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hTVKNMT:yJIxwd3BicHjvd5Bpd3K7bHH0bY9vKGSxc3XkJFMxKG2rboOgZoVnd3KnIITvJJN1cW23bHH0bY9vKHerdHigNVAmKHOncoXtJIZweiB3IH3pcpMh[nliaX3teY5w[myxdITpcochdWW2aH;kMEBKSzVyIE2gOE4zKM7:TT6= MojnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyN{izNVYoRjJ3MEe4N|E3RC:jPh?=
NG 108-15 NF;IdlBHfW6ldHnvckBie3OjeR?= Mk\VR49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDhZo9tcXOqIF3BVGsh[WO2aY\peJkhcW5ibX;1d4UhdmW3cn;icIF{fG:vYTDhcoQhemG2IHfsbY9u[SCqeXLybYQhVkdiMUC4MVE2KGOnbHzzMEBC[3Srdnn0fUA:KDFyIN88UU4> Mk\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3M{ezOVQoRjF3NUO3N|U1RC:jPh?=
PC12 Mk\qSpVv[3Srb36gZZN{[Xl? MkjENVAhfU1? MkjhOUBpenN? NIjDRmpC[3SrdnH0bY9vKG:oIF7y[lIwSVKHIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJGhQNTFicILveIVqdiCrbnT1Z5Rqd25iYYSgNVAhfU1iYX\0[ZIhPSCqcoOgdJJmfHKnYYTl[EB4cXSqIFrOT{BqdmirYnn0c5IhW1B4MECxNlUh\m:{IEGgbJIh[mWob4LlJINwdXCxdX7kJIFl\Gm2aX;uJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= Mk\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
PC12 MlfpSpVv[3Srb36gZZN{[Xl? M{TwcFExKHWP NFzoN2g2KGi{cx?= MofkRYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCPRVuyJIlvcGmkaYTvdkBWODF{NjDmc5IhOSCqcjDi[YZwemViY3;tdI92dmRiYXTkbZRqd25iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ M3f6b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{S1Olg2Lz5{MUO0OVY5PTxxYU6=
PC12 NYP0d4E1TnWwY4Tpc44h[XO|YYm= NIHL[VQyOCC3TR?= M3rxR|UhcHK| NG\US3lC[3SrdnH0bY9vKG:oIF7y[lIwSVKHIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJGhQNTFicILveIVqdiCrbnT1Z5Rqd25iYYSgNVAhfU1iYX\0[ZIhPSCqcoOgdJJmfHKnYYTl[EB4cXSqIF3FT|EhcW6qaXLpeI9zKFCGOUiwOVkh\m:{IEGgbJIh[mWob4LlJINwdXCxdX7kJIFl\Gm2aX;uJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2NU[4OUc,OjF|NEW2PFU9N2F-
HeLa MVvGeY5kfGmxbjDhd5NigQ>? NXH2U4xMOjBidV2= NFyz[o0{KGi{cx?= NEjDc4RKdmirYnn0bY9vKG:oIFXST|EwOiCyaH;zdIhwenmuYYTpc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCyNEB2VSCjZoTldkA{KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nz NUfNVI1kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A3OTVpPkKzOVcxPjF3PD;hQi=>
RD Moj5dWhVWyCjc4PhfS=> M1rCbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? M1PJeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MlzndWhVWyCjc4PhfS=> Ml\KdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= M2T2[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD MVzxTHRUKGG|c3H5 NVjOW|dueUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWkRiY3XscJM> MnvBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC NILwdo1yUFSVIHHzd4F6 MUDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= M37pRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32 MkjKdWhVWyCjc4PhfS=> NXTzWYp4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MDA-MB-231 MonhSpVv[3Srb36gZZN{[Xl? MnfCOVAhfU1? NFXPb5RFd3ewcnXneYxifGmxbjDv[kBOVVB{IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ NX;YW3NERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NlYzOjZpPkKyPVI3OjJ4PD;hQi=>
MDA-MB-231 Mm\TSpVv[3Srb36gZZN{[Xl? NV;wPJBiPTBidV2= MV;Ec5dvemWpdXzheIlwdiCxZjDNUXA6KGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{NkKyOkc,OjJ7Mk[yNlY9N2F-
Assay
Methods Test Index PMID
Western blot c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT ; p-JNK / JNK / Cyclin D1 ; p-HER2 / HER2 ; MMP9 / XIAP / VEGF 17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro MEK-inhibitory activity:

    Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.

Cell Research:

[1]

  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.

Animal Research:

[7]

  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.

Solubility (25°C)

In vitro

DMSO 14 mg/mL warmed
(52.37 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

1mg/mL

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
How to formulate this inhibitor for i.p. injection?

Answer:
You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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