PD98059

For research use only.

Catalog No.S1177

401 publications

PD98059 Chemical Structure

CAS No. 167869-21-8

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

Selleck's PD98059 has been cited by 401 publications

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Choose Selective MEK Inhibitors

Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  M1vibWZ2dmO2aX;uJGF{e2G7 NV7CUWt7OjEEoN88US=> MVSxJIg> MUTicI9kc3NiRGTDSE1qdmS3Y3XkJGRTPSCneIDy[ZN{cW:w NFjBdVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[5Olg3Oid-MkO2PVY5PjJ:L3G+
AML 1# NUHWeG5qSXCxcITvd4l{KEG|c3H5 NUXVb2RLOjEEoN88US=> MlfLNVIhcA>? NWLsW3I5\W6qYX7j[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBUOQ>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyNk[5NUc,OjN5ME[2PVE9N2F-
KG-1  NUG1d3RZSXCxcITvd4l{KEG|c3H5 M4HQW|IxyqEQvF2= M4iwPFEzKGh? MmjB[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDTNS=> NVjTZnh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFY3QTFpPkKzO|A3PjlzPD;hQi=>
HUVECs NW\YVHo4SXCxcITvd4l{KEG|c3H5 NIrwcJQzNzRizszN MVuyOE81QCCq MWXpcoR2[2W|IHPlcIwh\GWjdHi= M4DINVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3OVIxLz5{M{ewO|UzODxxYU6=
HepG2  M3X4TGZ2dmO2aX;uJGF{e2G7 NUHsZ442OjEEoN88US=> NIfJd2UzPCCq MlHDbY5pcWKrdIOgeIhmKEiRLUGgdJJwfGWrbjDlfJBz\XO|aX;uJINwNXS{ZXH0[YQhf2m2aDDt[ZRnd3KvaX6= Ml\GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe2NFkoRjJ|N{C3OlA6RC:jPh?=
NB4  MYfBdI9xfG:|aYOgRZN{[Xl? MWqxNE8zOC94MDFOwG0> M3TFfFEvPSCq NX;6R|FUTE2VTx?= MX;k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSnZDD3bZRpKFCjY3zpeIF5\Wx? M4\nRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{O1OVQyLz5{M{ezOVU1OTxxYU6=
BxPC-3 cells MkDFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnp[FUzOMLizszN NIP2VGwxNjViaB?= MVfpcohq[mm2czDWSWdHNUFvcnXneYxifGWmIFjVWmVEKGe{b4f0bEBidmRidIXi[UBnd3KvYYTpc44hcW6mdXPl[EBjgSCSQWKtNkBCWA>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd4NEC0Okc,OjN5NkSwOFY9N2F-
MKN45 Ml;TRZBweHSxc3nzJGF{e2G7 NXrsN4U{OTBizszNxsA> MU[yOEBp NILtW2pqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= NHfuTWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
SGC7901 NXTvbmM4SXCxcITvd4l{KEG|c3H5 NXrreZpDOTBizszNxsA> NWHESlJsOjRiaB?= NFfUSodqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
MKN45 MW\GeY5kfGmxbjDBd5NigQ>? MWCxNEDPxE4EoB?= MUeyOEBp MlfobY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[gdIhwe3Cqb4L5cIF1\WRiRWLLNU8z MmLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
SGC7901 Ml3WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[xUIhROTBizszNxsA> NUjUeW9mOjRxNEivO|IhcA>? NEm1c|RqdmirYnn0d{Bk\WyuIHfyc5d1cCClbz30doVifGWmIIfpeIghTEGSVB?= NIG3WHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
MKN45 NHPEWGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXrNVAh|ryPwrC= M1fZelI1NzR6L{eyJIg> MnHBbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[29vdILlZZRm\CC5aYToJGRCWFR? M4jX[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
SGC7901 NGTDcHJHfW6ldHnvckBCe3OjeR?= M4O3SVExKM7:TdMg M{XxZlI1KGh? MXXpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjDwbI9{eGixconsZZRm\CCHUluxM|I> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
HUASMCs M1zDc2Z2dmO2aX;uJGF{e2G7 NE\hfo8yOCEQvF5CpC=> NGXtdVUzPCCq M4D4T4RqdWmwaYPo[ZMhSW6pIFnJMYNifXOnZDDTU2NUOyCvUl7BJIFv\CCycn;0[YlvKGW6cILld5Nqd28EoB?= M2frZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG2OFY5Lz5{M{ixOlQ3QDxxYU6=
HUASMCs M{OzemZ2dmO2aX;uJGF{e2G7 MUmxNEDPxE4EoB?= M3zyT|I1KGh? NHrodXFqdmirYnn0d{BCdmdiSVmtbY5lfWOnZDDFVmsyNzJicHjvd5Bpd3K7bHH0bY9vKGyndnXs MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzNkS2PEc,OjN6MU[0Olg9N2F-
EPOR/CR3 NVnIOoVOTnWwY4Tpc44hSXO|YYm= MWC1NOKh|ryP NVnWenM5OyCq MWLy[YR2[2W|IFXQU{BidmRxb4KgTWwuOy2rbnT1Z4VlKHSqZTD0fZJwe2mwZTDwbI9{eGixconsZZRqd28EoB?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEezNUc,OjN6MkC3N|E9N2F-
NB4  MlLRSpVv[3Srb36gRZN{[Xl? MlnNNVAh|ryPwrC= M1exTlczKGh? NUmwNnFkTE2VTx?= NXjxXHl{cW6qaXLpeJMh\GG|YYTpcoljNWmwZIXj[YQhS0RzMXKg[ZhxemW|c3nvci=> NYPjRlY1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlU2QDVpPkKzPFI2PTh3PD;hQi=>
HL60  MmnRSpVv[3Srb36gRZN{[Xl? Ml;kNlDDqM7:TR?= M2C4fFczKGh? M4PmVWROW09? MkLCbY5pcWKrdIOg[IF{[XSrbnniMYlv\HWlZXSgR2QyOWJiZYjwdoV{e2mxbh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NUW4OUc,OjN6MkW1PFU9N2F-
HUVECs MkDXSpVv[3Srb36gRZN{[Xl? MWCyOUDPxE1? Ml3sNUBp NYjW[ZZDcW6lcnXhd4UhVkZvzsrCJJA3PSCwdXPs[YFzKHS{YX7zcI9k[XSrb36= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyMUCwPEc,OjN7MEGwNFg9N2F-
LNCaP  M4mwbmZ2dmO2aX;uJGF{e2G7 NUfHe4xLOTBizszNxsA> NHjqOIIyKGh? NFy1VnNl\WO{ZXHz[ZMhfGinIFXHSkB2eHKnZ4XsZZRm\CCyLWnCMVE> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh|OEOxPEc,OjN6M{izNVg9N2F-
HL-60 NVfab25JSXCxcITvd4l{KEG|c3H5 M3zNTFUxyqEQvF2= MWKxJIg> NULyN|BTemW|Y4Xld{BDSTF2NTDt[YRq[XSnZDDhdI9xfG:|aYO= M{np[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUS4O|UyLz5{M{m0PFc2OTxxYU6=
HepG2 NFrF[HJHfW6ldHnvckBCe3OjeR?= NFXscFY1OCEQvF2= MkPEOk8yOiCq M332SolvcGmkaYTzJJRp\SCrbnPy[YF{\SCxZjDwMWVTUzFiYX7kJJAu[y2MdX6gdJJwfGWrbjDlfJBz\XO|aX;uJIJ6KFCO NWnZclZiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFI5PTFpPkKzPVQzQDVzPD;hQi=>
HPAF-II NUiydYhSTnWwY4Tpc44hSXO|YYm= M{PORlExKM7:TdMg NUTsVohOPiCq M1vM[olv[3KnYYPlJI1qWi1zNEOg[ZhxemW|c3nvci=> MmPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O3NVAoRjJ|OUezO|ExRC:jPh?=
BxPC-3 MWnGeY5kfGmxbjDBd5NigQ>? NEDPXlgyOCEQvF5CpC=> MoPVOkBp NH\TWZZqdmO{ZXHz[UBucVJvMUSzJIV5eHKnc4Ppc44> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{exNEc,OjN7N{O3NVA9N2F-
COLO205 MXfBdI9xfG:|aYOgRZN{[Xl? MmHQNVAwOjBxNECg{txO NVXpWWxROjRiaB?= M1XKeolv\HWlZYOgSG5CKGyjZHTldkBnd3KvYYTpc44> NI\6OWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxPVExQCd-MkSwNVkyODh:L3G+
G292  NUPnb29tSXCxcITvd4l{KEG|c3H5 NFrDeY0{OMLizszN MYWyJIg> MoLXdoV{fG:{ZYOgZ4Fxe2GrY3nuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NX3TTnp1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVI6OzBpPkK0NFEzQTNyPD;hQi=>
SGC7901 NH3ycZNCeG:ydH;zbZMhSXO|YYm= M3n2OFIxyqEQvF2= MX2xJIg> NIq5W4hqdmirYnn0d{BieG:ydH;zbZMhcW6mdXPl[EBjgSCLRl6t{tHDqGGwZDC15qCzNUSIVWK= NWXhPYJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlc4PTBpPkK0NFI4PzVyPD;hQi=>
MGC803  MkT2RZBweHSxc3nzJGF{e2G7 NFXBOVgzOMLizszN NVvWWllmOSCq NVnnV5JTcW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliSV\OMe6yyqCjbnSgOgKBui2GRmXS MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB{N{e1NEc,OjRyMke3OVA9N2F-
HPMC M{nSU2Fxd3C2b4Ppd{BCe3OjeR?= NXTxPYpqOTEEoN88US=> M162R|I1yqCq NEnBeodz\X[ncoPld{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KEiJUFTT MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB2MkizPEc,OjRyNEK4N|g9N2F-
HCT-8 M3TJNGFxd3C2b4Ppd{BCe3OjeR?= NXH0T3JpOTEEoN88US=> NYTnZ497OjUEoHi= M37FOoRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUi2N{c,OjRyOUW4OlM9N2F-
HPMC NEXze4pHfW6ldHnvckBCe3OjeR?= M33Z[FExyqEQvF2= NWHwZXRpPDhiaB?= NWjDZW1FemW4ZYLz[ZMhfGinIHPoZY5o\XNiaX6gZ4VtdCCvb4LwbI9td2e7IHnu[JVk\WRiYomgTGdRTFN? Mn3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNEK4N|goRjJ2MESyPFM5RC:jPh?=
A549 MVTGeY5kfGmxbjDBd5NigQ>? Mnm1OVDDqM7:TR?= MUSyJIg> NF7KfpBjdG:la4OgSXJMKHCqb4PwbI9zgWyjdHnvckBu\WSrYYTl[EBjgSBzLEKtUnE> M1zycVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2ME[3O|I4Lz5{NEC2O|czPzxxYU6=
Caco-2  MkW4RZBweHSxc3nzJGF{e2G7 NHL6W44yOMLizszN NXHmToZHOjUEoHi= M2HudoRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? NWXlfZlPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVU5PjNpPkK0NFk2QDZ|PD;hQi=>
AGS NXLFNpM5TnWwY4Tpc44hSXO|YYm= MnrVNVAh|ryPwrC= MX6wMlUhcA>? M{i1eYlvcGmkaYTzJJRp\SC3cILl[5Vt[XSrb36gc4YhfGinIFnMMVgh\2WwZR?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNkG2Okc,OjRzME[xOlY9N2F-
Ca9-22 NIPqTWhHfW6ldHnvckBCe3OjeR?= MV2zJO69VQ>? M3rJblEwOiCq NYDpeXJDemWmdXPld{BJ[lJvaX7keYNm\CCDVF[tNkBxcG:|cHjvdplt[XSrb36= MnWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[1N|IoRjJ2MUK2OVMzRC:jPh?=
Ca9-22 M37PemZ2dmO2aX;uJGF{e2G7 MlTjN{DPxE1? NH6wWocyKGh? Ml32ZYJwdGm|aHXzJJRp\SCjYnnsbZR6KG:oIFjiVkB1dyCrbnT1Z4UhUUxvODDwdo9lfWO2aX;u MkDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[1N|IoRjJ2MUK2OVMzRC:jPh?=
HAECs MnzjSpVv[3Srb36gRZN{[Xl? MlTINVAh|ryP MXexJIg> M2nkXOKh[XS2ZX71ZZRmeyCWTl[t{tEue3SrbYXsZZRm\CCLQ1HNMVEh[W6mIG\DRW0uOSCneIDy[ZN{cW:w NXrUR5pHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|Q3PTdpPkK0NVM1PjV5PD;hQi=>
Caco-2 NV20eGFTTnWwY4Tpc44hSXO|YYm= MVm1NOKh|ryP MoXJOFhp MXjEUXNQ NFrqfpBmdmijbnPld{B1cGVibWLORUBt\X[nbIOgc4bDqFOFTl6xRUxHYFmGMz|CpGxEXC{EoFzPXEzDqEiLRkPBMOKhYkdzNj|CpHBFTT[DwrDhcoTDqEyJQVzTNVbDqGenbnXzxsBkdy22cnXheIVlKHerdHigSIV5 Moj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkG2PVUoRjJ2MU[xOlk2RC:jPh?=
MCF-7 NVLpZoVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUixNEDPxE1? MlXpOFhp MWfEUXNQ M4nNOpJmfmW{c3XzJGJPTi2rbnT1Z4VlKGOnbHygZ5lkdGViYYLy[ZN1 MmDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkO0NFQoRjJ2MU[zOFA1RC:jPh?=
SMMC7721 M4nVPWZ2dmO2aX;uJGF{e2G7 NIXFVpozPS93MDFOwG0> NEfQW4UzPCCq NHL2Xlh{fXCycnXzd4V{KHSqZTDlfJBz\XO|aX;uJI9nKHBvQXv0JI9zKHBvRWLLNU8zyqB? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4OEC1Okc,OjRzNkiwOVY9N2F-
SGC7901  MoH6RZBweHSxc3nzJGF{e2G7 MWW1NOKh|ryP NIfV[GkzPC92OD:3NkBp M3K5SGROW09? Mn2xbY5lfWOnczDhdI9xfG:|aYOgZ49u[mmwZXSge4l1cCCMQVuyJJNpWk6D NXf5ZZdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|gzPDBpPkK0NVc5OjRyPD;hQi=>
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DLD-1  MoP3SpVv[3Srb36gRZN{[Xl? NHPQ[WszOMLizszN MXS0PEBp NHjsSmpz\WS3Y3XzJJRp\SCETlnQN{BmgHC{ZYPzbY9vKHC{ZT30doVifGWmIIfpeIghPS2jenGt[GM> NGPjeVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVU5OSd-MkSyNVE2QDF:L3G+
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SGC-7901  Mmr6RZBweHSxc3nzJGF{e2G7 MmrnNlAh|ryP M3vINlI1KGh? NWK5bIo6cW6qaXLpeJMhS1BvbXXkbYF1\WRiYYDvdJRwe2m| MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2MUO1NUc,OjR{NEGzOVE9N2F-
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KATOIII  MnXVSpVv[3Srb36gRZN{[Xl? NUX4e2JqPTBizszN MnPROFghcA>? MX3EUXNQ MUjpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| NGPkO409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
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KYSE30 M3rBPGZ2dmO2aX;uJGF{e2G7 NWXVcHZPOjBxNUCvNVAxKM7:TR?= NHr5[WE1QCCq MUjEUXNQ M1j2eYlvcGmkaYTzJJAuTXKtIHHu[EB4d3K2bXHucolvKGSxd37y[Yd2dGG2ZXSgdE1Cc3RiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NVztPJpRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE1 MoXZSpVv[3Srb36gRZN{[Xl? NHHZVmQzOC93MD:xNFAh|ryP NES0Rng1QCCq M3jvNmROW09? NFPmOWhqdmirYnn0d{BxNUW{azDhcoQhf2:{dH3hco5qdiCmb4fudoVofWyjdHXkJJAuSWu2IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NWHXV2Q3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE4 M{XWXGZ2dmO2aX;uJGF{e2G7 M1jve|IxNzVyL{GwNEDPxE1? M334TFQ5KGh? NHvHOW5FVVOR NX\PXZBGcW6qaXLpeJMheC2HcnugZY5lKHexcoTtZY5vcW5iZH;3cpJm\3WuYYTl[EBxNUGtdDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= M1\FTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
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HepG2 NFnQWpFCeG:ydH;zbZMhSXO|YYm= NUC1dY9zOjBizszN MWGyOEBp M1Ll[IlvcGmkaYTzJGVTUzFxMjDwbI9{eGixconsZZRqd25iYX7kJIVvcGGwY3XzJHZDOS2rbnT1Z4VlKGGyb4D0c5Nqew>? MkX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NEe5NFkoRjJ2MkS3PVA6RC:jPh?=
HepG2 NETNO2VHfW6ldHnvckBCe3OjeR?= NI\0V4kzOCEQvF2= NE\xfmczKGh? NFr3c2JmdmijbnPld{BXSjFvaX7keYNm\CCIT2jPN4EhfHKjboPjdolxfGmxbnHsJIFkfGm4aYT5 NHTjUJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0O|kxQSd-MkSyOFc6ODl:L3G+
MC-3 M1TWdmFxd3C2b4Ppd{BCe3OjeR?= NUDRXJRvOTBizszN NF\JXY8zPCCq M1PIZ5BwfGWwdHnheIVlKE2HU1OtbY5lfWOnZDDhdI9xfG:|aYOgbY4hKGOnbHzz NWLado13RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|A2OjNpPkK0NlcxPTJ|PD;hQi=>
Raji  MVvGeY5kfGmxbjDBd5NigQ>? M3TKdFExKM7:TR?= NHfiR5QyKGh? MXPicI9kc3NiaIPCRWZHKGmwZIXj[YQhTXKtMT:yJJBpd3OyaH;yfYxifGmxbh?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4OU[zNEc,OjR{Nkm2N|A9N2F-
Raji  M3PXW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLYcop6OTBizszN NEDiRoQyKGh? MXHpcohq[mm2czD0bIUh[mG|YXygc5IhcHOEQV\GMZN1cW23bHH0[YQh[2WubDDwdo9tcW[ncnH0bY9vKGGwZDD2bYFjcWyrdIm= NHjzPIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVY{OCd-MkSyOlk3OzB:L3G+
A549 M1LPNWZ2dmO2aX;uJGF{e2G7 MUKzNEDPxE1? M1XzRlAvPSCq MUfEUXNQ MV;pcohq[mm2czD0bJJwdWKrbj3pcoR2[2WmIFOvSWJR|rJiVHjyNlM2yqCyaH;zdIhwenmuYYTpc44> MofTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{N{e2PVYoRjJ2Mke3Olk3RC:jPh?=
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PANC-1 M{fsUGZ2dmO2aX;uJGF{e2G7 NUToXXYzOjBizszN MkTBOFghcA>? NHn6VmNqdmirYnn0d{B1cGViZYjwdoV{e2mxbjDv[kDPnD[GIHnuJJJme3CxboPlJJRwKHSqZTDQVGFT|rUEoHHnc45qe3UEoB?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7NEGzN{c,OjR{OUSxN|M9N2F-
A549 NX\iN4VzTnWwY4Tpc44hSXO|YYm= NV;vVZVGOzBizszN NWPFZWh3OC53IHi= NWXKWWJMTE2VTx?= NWmxVplicW6qaXLpeJMhfGinIITodo9u[mmwLXnu[JVk\WRiSVytPE9EYEOOOD3MeYMh[WO2aY\peJk> NXnlUWk2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|c3QTZpPkK0Nlc4Pjl4PD;hQi=>
SW480 NFj4NndHfW6ldHnvckBCe3OjeR?= NGP1W40yOCEQvF2= NIO4[WwzOCCq MYTEUXNQ NF;ZXnZ{fXCycnXzd4V{KHSqZTDDVnQh[WO2aY\peJk> M1LGWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 MVvGeY5kfGmxbjDBd5NigQ>? Ml3ENVAh|ryP M3TGVlUhcA>? MWnEUXNQ MX7pcohq[mm2czDXcpQucW6mdXPl[EDPui2lYYTlcolvN1SFRkSgZYN1cX[rdImgZY5lKG63Y3zlZZIh|rJvY3H0[Y5qdiCjY3P1cZVt[XSrb36= MmTjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkSzOlYoRjJ2M{K0N|Y3RC:jPh?=
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HepG2  NFHkb5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHIT4QzOCEQvF2= NX7hR4dNOjRiaB?= MWHzeZBxemW|c3XzJHRITi4QskGtbY5lfWOnZDDj[YxtKHC{b3zp[oVz[XSrb36gZY5lKGmwdnHzbY9v MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV4MES4PEc,OjV3NkC0PFg9N2F-
SW480 M13jbmZ2dmO2aX;uJGF{e2G7 NI\h[pkzOMLizszN NYrVNpFIOcLiaB?= MXry[YR2[2W|IITo[UBmgHC{ZYPzbY9vKG:oIFHUSlMheHKxdHXpci=> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2N{ixOkc,OjV2NEe4NVY9N2F-
MDA-MB-231 Mor4SpVv[3Srb36gRZN{[Xl? MmHVNlUh|ryP MW[yMVMhcA>? M4TodoRm[3KnYYPld{BxNUWUS{GvNkBidmRiU{GwNGE1KGW6cILld5Nqd25? M3j1SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW1PFc2Lz5{NUW1OVg4PTxxYU6=
HepG2  MVnGeY5kfGmxbjDBd5NigQ>? MUOxNEDPxE1? NYXnUJp1PSCq M37PS4Jtd2OtczDwbI9{eGixconsZZRm\CCPQWDLd{BqdmS3Y3XkJIJ6KGW6b3flco92eyCWR1[t{tIy NW\xNndTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OlA1QDhpPkK1OVYxPDh6PD;hQi=>
MCF-7  M{TuUWZ2dmO2aX;uJGF{e2G7 NWi3Z5dXOTBizszN NFe1enYyKGh? NFzZOlRqdmirYnn0d{BKVC1zOD3lcohidmOnZDDj[YxtKG2rZ4LheIlwdg>? M2jBdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{K3NFEyLz5{NUeyO|AyOTxxYU6=
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PC12 NXfhNJdUTnWwY4Tpc44h[XO|YYm= NFPJWJUyOCC3TR?= MmrjOUBpenN? M1\URWFkfGm4YYTpc44hd2ZiToLmNk9CWkViaX6gdoF1KFCFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgTG8uOSCycn;0[YlvKGmwZIXjeIlwdiCjdDCxNEB2VSCjZoTldkA2KGi{czDwdoV1emWjdHXkJJdqfGhiTVXLNkBqdmirYnn0c5IhXTBzMk[g[o9zKDFiaIKgZoVnd3KnIHPvcZBwfW6mIHHk[Il1cW:wIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2NU[4OUc,OjF|NEW2PFU9N2F-
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HeLa NF3TfFZHfW6ldHnvckBie3OjeR?= M{nFW|IxKHWP NIXrZW8{KGi{cx?= MYLJcohq[mm2aX;uJI9nKEWUS{GvNkBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCB{MDD1UUBi\nSncjCzJIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| Mn3OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{C2NVUoRjJ|NUewOlE2RC:jPh?=
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NB1643 NHH0fpRyUFSVIHHzd4F6 M1HX[pFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= NWTOTmtnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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SK-N-MC NWrTeJJbeUiWUzDhd5NigQ>? NUTwNmhHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> M3:1d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32 MUPxTHRUKGG|c3H5 M1TiSpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> MnjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MDA-MB-231 NYLVNGdyTnWwY4Tpc44h[XO|YYm= M2\kZ|UxKHWP NYHVXnp[TG:5boLl[5Vt[XSrb36gc4YhVU2SMjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFUxKHWPIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NGj4NpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+
MDA-MB-231 NULq[2pPTnWwY4Tpc44h[XO|YYm= NYDCdYRqPTBidV2= MUjEc5dvemWpdXzheIlwdiCxZjDNUXA6KGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NF60VoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol

Kinase Assay:

[1]

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In vitro MEK-inhibitory activity:

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.
Cell Research:

[1]

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  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.


    (Only for Reference)
Animal Research:

[7]

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  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (52.37 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to formulate this inhibitor for i.p. injection?

  • Answer:

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID