PD98059

Catalog No.S1177

For research use only.

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

PD98059 Chemical Structure

CAS No. 167869-21-8

Selleck's PD98059 has been cited by 435 publications

Purity & Quality Control

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Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  Ml\iSpVv[3Srb36gRZN{[Xl? M{DCUlIxyqEQvF2= MlPoNUBp MXzicI9kc3NiRGTDSE1qdmS3Y3XkJGRTPSCneIDy[ZN{cW:w NGTZWYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[5Olg3Oid-MkO2PVY5PjJ:L3G+
AML 1# M3ruT2Fxd3C2b4Ppd{BCe3OjeR?= M2r2NlIxyqEQvF2= NGLuZ|YyOiCq M{[wdIVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgV|E> Mn;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5ME[2PVEoRjJ|N{C2OlkyRC:jPh?=
KG-1  NFrvOWFCeG:ydH;zbZMhSXO|YYm= NHH4TY4zOMLizszN MlLLNVIhcA>? Mmnp[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDTNS=> NVTINIUxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFY3QTFpPkKzO|A3PjlzPD;hQi=>
HUVECs NEfNZ3RCeG:ydH;zbZMhSXO|YYm= M4jjWVIwPCEQvF2= MVKyOE81QCCq MlrCbY5lfWOnczDj[YxtKGSnYYTo M1PLVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3OVIxLz5{M{ewO|UzODxxYU6=
HepG2  NEX4VGtHfW6ldHnvckBCe3OjeR?= NWHLUHUyOjEEoN88US=> MYqyOEBp NXvKd3k1cW6qaXLpeJMhfGinIFjPMVEheHKxdHXpckBmgHC{ZYPzbY9vKGOxLYTy[YF1\WRid3n0bEBu\XSob4LtbY4> NYPHPZB{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc3ODlpPkKzO|A4PjB7PD;hQi=>
NB4  M1Tj[WFxd3C2b4Ppd{BCe3OjeR?= NGDv[JUyOC9{MD:2NEDPxE1? NF7nOpAyNjViaB?= NEnyfnFFVVOR Ml7p[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHPvMZRz\WG2ZXSge4l1cCCSYXPsbZRigGWu Mn;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5M{W1OFEoRjJ|N{O1OVQyRC:jPh?=
BxPC-3 cells MkKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUmxPVZTOjEEoN88US=> MnPPNE42KGh? NXHXW2l2cW6qaXLpeJMhXkWJRj3BMZJm\3WuYYTl[EBJXV[HQzDndo94fGhiYX7kJJR2[mViZn;ycYF1cW:wIHnu[JVk\WRiYomgVGFTNTJiQWC= MlfjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NkSwOFYoRjJ|N{[0NFQ3RC:jPh?=
MKN45 NFvxNYlCeG:ydH;zbZMhSXO|YYm= Mn7nNVAh|ryPwrC= Mki3NlQhcA>? MYHpcoNz\WG|ZYOgeIhmKESDUGStbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? NUewS2hjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 NGSyTnJCeG:ydH;zbZMhSXO|YYm= MWSxNEDPxE4EoB?= M3u0XFI1KGh? MWjpcoNz\WG|ZYOgeIhmKESDUGStbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? MmCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 M4PKTWZ2dmO2aX;uJGF{e2G7 NXvONlV6OTBizszNxsA> NIrQTWwzPCCq M1uwbIlvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIFXST|EwOg>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 NEPOVYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M134OlExKM7:TdMg NHLKeXAzPC92OD:3NkBp NVPPWJRscW6qaXLpeJMh[2WubDDndo94fGhiY3:teJJm[XSnZDD3bZRpKESDUGS= MnrJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 M4DM[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWSxNEDPxE4EoB?= MYiyOE81QC95MjDo M1vrT4lvcGmkaYTzJINmdGxiZ4Lve5RpKGOxLYTy[YF1\WRid3n0bEBFSVCW MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 M{DoVGZ2dmO2aX;uJGF{e2G7 NWWy[mZ[OTBizszNxsA> M3HVeVI1KGh? NG\pUldqdmirYnn0d{B1cGViZYjwdoV{e2mxbjDv[kBxcG:|cHjvdplt[XSnZDDFVmsyNzJ? NXXxXHhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
HUASMCs MmP6SpVv[3Srb36gRZN{[Xl? MlzINVAh|ryPwrC= MXeyOEBp MUfkbY1qdmm|aHXzJGFv\yCLST3jZZV{\WRiU1;DV|MhdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vyqB? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhzNkS2PEc,OjN6MU[0Olg9N2F-
HUASMCs NGDpRWhHfW6ldHnvckBCe3OjeR?= MkjVNVAh|ryPwrC= NV32bmhXOjRiaB?= NHXJ[GRqdmirYnn0d{BCdmdiSVmtbY5lfWOnZDDFVmsyNzJicHjvd5Bpd3K7bHH0bY9vKGyndnXs NGL6bXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ixOlQ3QCd-MkO4NVY1Pjh:L3G+
EPOR/CR3 NVPpUVd1TnWwY4Tpc44hSXO|YYm= MWS1NOKh|ryP M3PlRlMhcA>? NH7xNHpz\WS3Y3XzJGVRVyCjbnSvc5IhUUxvMz3pcoR2[2WmIITo[UB1gXKxc3nu[UBxcG:|cHjvdplt[XSrb39CpC=> MkfQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkC3N|EoRjJ|OEKwO|MyRC:jPh?=
NB4  NGXFNJNHfW6ldHnvckBCe3OjeR?= NG\SbYsyOCEQvF5CpC=> NFvGZ|Y4OiCq NHfaeVBFVVOR MWjpcohq[mm2czDkZZNifGmwaXKtbY5lfWOnZDDDSFEy[iCneIDy[ZN{cW:w NF;JU|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOVU5PSd-MkO4NlU2QDV:L3G+
HL60  NVnmOGNnTnWwY4Tpc44hSXO|YYm= M3HY[FIxyqEQvF2= NUPRZpM{PzJiaB?= M2TSZmROW09? M2PMZolvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= MkDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkW1PFUoRjJ|OEK1OVg2RC:jPh?=
HUVECs NYrsNGdxTnWwY4Tpc44hSXO|YYm= MoXuNlUh|ryP M1r4R|EhcA>? NUjERVVjcW6lcnXhd4UhVkZvzsrCJJA3PSCwdXPs[YFzKHS{YX7zcI9k[XSrb36= MnLMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEGwNFgoRjJ|OUCxNFA5RC:jPh?=
LNCaP  MlnSSpVv[3Srb36gRZN{[Xl? MUCxNEDPxE4EoB?= NGfjcmgyKGh? NGH3TmFl\WO{ZXHz[ZMhfGinIFXHSkB2eHKnZ4XsZZRm\CCyLWnCMVE> M1WxW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEO4N|E5Lz5{M{izPFMyQDxxYU6=
HL-60 NVjVbXU6SXCxcITvd4l{KEG|c3H5 NIHpcHE2OMLizszN NVLSV406OSCq M3nSU5Jme2O3ZYOgRmEyPDVibXXkbYF1\WRiYYDvdJRwe2m| NETpRXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0PFc2OSd-MkO5OFg4PTF:L3G+
HepG2 MV3GeY5kfGmxbjDBd5NigQ>? MorUOFAh|ryP NGq1WJk3NzF{IHi= NH7lOJVqdmirYnn0d{B1cGViaX7jdoVie2Vib3[gdE1GWktzIHHu[EBxNWNvSoXuJJBzd3SnaX6g[ZhxemW|c3nvckBjgSCSTB?= M136dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUSyPFUyLz5{M{m0Nlg2OTxxYU6=
HPAF-II NVO2RWU4TnWwY4Tpc44hSXO|YYm= MkTjNVAh|ryPwrC= NWD6PWNOPiCq NVS1co5{cW6lcnXhd4UhdWmULUG0N{BmgHC{ZYPzbY9v MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{exNEc,OjN7N{O3NVA9N2F-
BxPC-3 NXfieWUxTnWwY4Tpc44hSXO|YYm= NIPtXHQyOCEQvF5CpC=> MYK2JIg> NUKxeZVicW6lcnXhd4UhdWmULUG0N{BmgHC{ZYPzbY9v MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{exNEc,OjN7N{O3NVA9N2F-
COLO205 MVvBdI9xfG:|aYOgRZN{[Xl? M1\NWFExNzJyL{SwJO69VQ>? MoPaNlQhcA>? MWDpcoR2[2W|IFTORUBt[WSmZYKg[o9zdWG2aX;u NHq3UYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxPVExQCd-MkSwNVkyODh:L3G+
G292  M{mwdGFxd3C2b4Ppd{BCe3OjeR?= MVqzNOKh|ryP NGn0U4szKGh? NGfoXmpz\XO2b4Lld{Bk[XC|YXnjbY4ucW6mdXPl[EBk\WyuIHTlZZRp Mni5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUK5N|AoRjJ2MEGyPVMxRC:jPh?=
SGC7901 NGO3PJNCeG:ydH;zbZMhSXO|YYm= M4TKU|IxyqEQvF2= MmT6NUBp M2\qZYlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= NWjzb3R6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlc4PTBpPkK0NFI4PzVyPD;hQi=>
MGC803  NUjhRoFrSXCxcITvd4l{KEG|c3H5 M3;pPFIxyqEQvF2= MWKxJIg> MoDDbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhUU[QLd8xxsBidmRiNfMAtk1FTlWU NImxe|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyO|c2OCd-MkSwNlc4PTB:L3G+
HPMC M{PhbmFxd3C2b4Ppd{BCe3OjeR?= M3:4XVExyqEQvF2= Mo\jNlTDqGh? MkjwdoV3\XK|ZYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBJT1CGUx?= M2j1flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
HCT-8 NU\BXlNQSXCxcITvd4l{KEG|c3H5 M4HlTlExyqEQvF2= M2K5S|I1yqCq MXnk[YNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTC1MWZW MlzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW4OlMoRjJ2MEm1PFY{RC:jPh?=
HPMC M3KzO2Z2dmO2aX;uJGF{e2G7 MoLZNVDDqM7:TR?= M1jqXFQ5KGh? MkTFdoV3\XK|ZYOgeIhmKGOqYX7n[ZMhcW5iY3XscEBud3KyaH;sc4d6KGmwZIXj[YQh[nliSFfQSHM> NX;uTFJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOFI5OzhpPkK0NFQzQDN6PD;hQi=>
A549 NWjoOoNiTnWwY4Tpc44hSXO|YYm= NEnEbHU2OMLizszN NH;1T5gzKGh? MmLYZoxw[2u|IFXST{BxcG:|cHjvdplt[XSrb36gcYVlcWG2ZXSgZpkhOSx{LV7R NHX5e4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2O|czPyd-MkSwOlc4Ojd:L3G+
Caco-2  M1X4SGFxd3C2b4Ppd{BCe3OjeR?= Mln4NVDDqM7:TR?= M3i1c|I1yqCq Mknj[IVkemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgOU1HXQ>? M1rPVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1PFY{Lz5{NEC5OVg3OzxxYU6=
AGS M4XKWGZ2dmO2aX;uJGF{e2G7 NHPCWXAyOCEQvF5CpC=> M4TWSFAvPSCq NGnKOZJqdmirYnn0d{B1cGVidYDy[Yd2dGG2aX;uJI9nKHSqZTDJUE05KGenbnW= M4DpZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUC2NVY3Lz5{NEGwOlE3PjxxYU6=
Ca9-22 NIjBbodHfW6ldHnvckBCe3OjeR?= NVztdJl4OyEQvF2= NHvvdlQyNzJiaB?= M3Hk[5Jm\HWlZYOgTIJTNWmwZIXj[YQhSVSILUKgdIhwe3Cqb4L5cIF1cW:w NY\vdGtURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY2OzJpPkK0NVI3PTN{PD;hQi=>
Ca9-22 M2H4WGZ2dmO2aX;uJGF{e2G7 MoLCN{DPxE1? M2TjeVEhcA>? NF7tUWpi[m:uaYPo[ZMhfGinIHHibYxqfHlib3[gTIJTKHSxIHnu[JVk\SCLTD24JJBzd2S3Y4Tpc44> NHH6eZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOlU{Oid-MkSxNlY2OzJ:L3G+
HAECs NX:2cllpTnWwY4Tpc44hSXO|YYm= MlvxNVAh|ryP NFjpdpMyKGh? NW\oRYM{yqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|NE[1O{c,OjRzM{S2OVc9N2F-
Caco-2 NF:yPIlHfW6ldHnvckBCe3OjeR?= MVi1NOKh|ryP MoPTOFhp NUS1T4l[TE2VTx?= MYrlcohidmOnczD0bIUhdVKQQTDs[ZZmdHNib3dCpHNEVk5zQTzGXHlFOy{EoFzDWEzDqEyRWD|CpGhKTjODLNMgXmcyPi{EoGDESVZCyqCjbnVCpGxISUyVMUdCpIdmdmW|wrDjc{11emWjdHXkJJdqfGhiRHX4 M1fTbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[xOlk2Lz5{NEG2NVY6PTxxYU6=
MCF-7 NVnNWFU6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\BbFE6OTBizszN M4jsRlQ5cA>? Mk\QSG1UVw>? MkXldoV3\XK|ZYOgRm5HNWmwZIXj[YQh[2WubDDjfYNt\SCjcoLld5Q> NVO1OmdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlM1ODRpPkK0NVY{PDB2PD;hQi=>
SMMC7721 MWXGeY5kfGmxbjDBd5NigQ>? MlLzNlUwPTBizszN MVuyOEBp M4TIPZN2eHC{ZYPz[ZMhfGinIHX4dJJme3Orb36gc4YheC2Da4Sgc5IheC2HUluxM|LDqA>? NV3kRmhbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlgxPTZpPkK0NVY5ODV4PD;hQi=>
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KYSE30 MVHGeY5kfGmxbjDBd5NigQ>? MXm1NEDPxE1? MV:0PEBp M1PvNGROW09? MUL1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBJVEFiY3zhd5MhUQ>? MofNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
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TE1 NYeyWIZzTnWwY4Tpc44hSXO|YYm= MVKyNE82OC9zMECg{txO NGnQT3g1QCCq M4T5[GROW09? M3T1UIlvcGmkaYTzJJAuTXKtIHHu[EB4d3K2bXHucolvKGSxd37y[Yd2dGG2ZXSgdE1Cc3RiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NVPjZ29HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
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HT29 MoTnSpVv[3Srb36gRZN{[Xl? M3\5UlExKM7:TR?= M2[4W|IhcA>? MWXpcohq[mm2czDv[kBLSUt{LDDFVmsyNzJiYX7kJHNVSVR|IIDoc5NxcG:{eXzheIlwdg>? NH7hUXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2OVI6Oyd-MkSyOlUzQTN:L3G+
HepG2 NHfrUW5CeG:ydH;zbZMhSXO|YYm= NFW5S2ozOCEQvF2= MVmyOEBp M4nXVIlvcGmkaYTzJGVTUzFxMjDwbI9{eGixconsZZRqd25iYX7kJIVvcGGwY3XzJHZDOS2rbnT1Z4VlKGGyb4D0c5Nqew>? NF3B[VU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0O|kxQSd-MkSyOFc6ODl:L3G+
HepG2 Mn7lSpVv[3Srb36gRZN{[Xl? MYCyNEDPxE1? MkK1NkBp MmHY[Y5p[W6lZYOgWmIyNWmwZIXj[YQhTk:[T{PhJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eR?= MlnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NEe5NFkoRjJ2MkS3PVA6RC:jPh?=
MC-3 M2K5N2Fxd3C2b4Ppd{BCe3OjeR?= M3XSTFExKM7:TR?= MkntNlQhcA>? MoLIdI91\W62aXH0[YQhVUWVQz3pcoR2[2WmIHHwc5B1d3OrczDpckAh[2WubIO= NEDJZ249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3NFUzOyd-MkSyO|A2OjN:L3G+
Raji  M1HkSWZ2dmO2aX;uJGF{e2G7 NUi3eHBKOTBizszN MUCxJIg> Mlm2Zoxw[2u|IHjzRmFHTiCrbnT1Z4VlKEW{a{GvNkBxcG:|cHjvdplt[XSrb36= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4OU[zNEc,OjR{Nkm2N|A9N2F-
Raji  M4faWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjG[Xp6OTBizszN M3jaOlEhcA>? NGfue3FqdmirYnn0d{B1cGViYnHzZYwhd3JiaIPCRWZHNXO2aX31cIF1\WRiY3XscEBxem:uaX\ldoF1cW:wIHHu[EB3cWGkaXzpeJk> M4Cx[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[5OlMxLz5{NEK2PVY{ODxxYU6=
A549 NH62NXJHfW6ldHnvckBCe3OjeR?= NXLldJIxOzBizszN Mmm1NE42KGh? NF\qVGRFVVOR MoPvbY5pcWKrdIOgeIhzd22kaX6tbY5lfWOnZDDDM2VDWM7{IGTodlI{PcLicHjvd5Bpd3K7bHH0bY9v MmTjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{N{e2PVYoRjJ2Mke3Olk3RC:jPh?=
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A549 NF3ESFhHfW6ldHnvckBCe3OjeR?= MVuzNEDPxE1? M33z[FAvPSCq NVe1Not[TE2VTx?= NFLUd4FqdmirYnn0d{B1cGVidHjyc41jcW5vaX7keYNm\CCLTD24M2NZS0x6LVz1Z{Bi[3Srdnn0fS=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5N{[5Okc,OjR{N{e2PVY9N2F-
SW480 NVTaS5oxTnWwY4Tpc44hSXO|YYm= M{TYblExKM7:TR?= NUjsZZFZOjBiaB?= Ml7sSG1UVw>? M335V5N2eHC{ZYPz[ZMhfGinIFPSWEBi[3Srdnn0fS=> NW\tSpZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlQ{PjZpPkK0N|I1OzZ4PD;hQi=>
HEK 293 NITwS3FHfW6ldHnvckBCe3OjeR?= M3zqRlExKM7:TR?= M{XqUFUhcA>? NEewUG9FVVOR NF34bYxqdmirYnn0d{BYdnRvaX7keYNm\CEQsj3jZZRmdmmwL2TDSlQh[WO2aY\peJkh[W6mIH71Z4xm[XJizsKtZ4F1\W6rbjDhZ4N2dXWuYYTpc44> M4nPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 NX\mV2VOTnWwY4Tpc44hSXO|YYm= NWGxWGt[OTBizszN NGTC[Wg2KGh? Mk\ySG1UVw>? NH3GSm9{fXCycnXzd4V{KHSqZTDDVnQh[WO2aY\peJk> M{jlflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HL-60  NV75RlFzTnWwY4Tpc44hSXO|YYm= MoH3NVAwOjBizszN NV;jOXF6OSCq MVnpcohq[mm2czD0bIXDqE5wIHPobY5mdnOrc3X4eJJi[3UEoHnu[JVk\WRiZHnm[oVz\W62aXH0bY9vKGmwdH:g[5JidnWub3P5eIV{ NYfXfm1oRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOVcxOjBpPkK0N|U4ODJyPD;hQi=>
HL-60 MX\GeY5kfGmxbjDBd5NigQ>? NFLu[|kzKML3TR?= M{ewN|E3KGh? MnHlSG1UVw>? NWGxUW5icW6qaXLpeJMhfGinIHHzd49kcWG2aX;uJI9nKHCVNkKxJHJi\i1zIHHu[EBPTkGWY{OsJIFv\CC2aHWgVmEucW6mdXPl[EBxcG:|cHjvdplt[XSrb36gc4YhdnWlbHXhdkBPTkGWY{O= NXnKUIlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzN|AxPjhpPkK0N|MxODZ6PD;hQi=>
HeLa MXvGeY5kfGmxbjDBd5NigQ>? NVq5UVNGPTBizszN NYnSUZlROC53IHi= NWP4WZpD[myxY3vzJHRTYC1zIH71Z4xm[XJibXnndoF1cW:wIHHu[EBVYE6LUDDkc5dvNXKnZ4XsZZRqd25? NFPFb5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3OlgzPyd-MkSzO|Y5Ojd:L3G+
HUVECs MV7GeY5kfGmxbjDBd5NigQ>? MYCxNOKh|ryP MVmxJIg> NEnqW4NqdmirYnn0d{B1cGViSFTMJJJm\HWlZXSgR29ZNTJiZYjwdoV{e2mxbjDhcoQhWEeLLUKgdoVt\WG|ZR?= NILMN4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO4OVExQSd-MkSzPFUyODl:L3G+
MCF-7 NXjk[ZlCTnWwY4Tpc44hSXO|YYm= MUGxNOKh|ryP NInjPWEyOC9|MDDtbY4> MXXy[YR2[2W|IITo[UBWXFBvZHXw[Y5l\W62IFXST{BxcG:|cHjvdplt[XSrb36= NVK4NmJWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPVA5OTlpPkK0N|kxQDF7PD;hQi=>
HGC-27 M4HQVWFxd3C2b4Ppd{BCe3OjeR?= NFjL[ZYyKML3TR?= M3XQflEhcA>? NUnHTHpQe3WycILld5NmeyCUQVSwNFEheGy3czDNT{0zOjB4LXnu[JVk\WRiY3XscEB3cWGkaXzpeJkhdG:|cx?= NFvaUVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESxOlM1QSd-MkS0NVY{PDl:L3G+
PC3  NYLBTJJzSXCxcITvd4l{KEG|c3H5 MUG1NEDPxE1? MWiwMlUhcA>? Mn2wbY5pcWKrdIOgUWh[NTR2OT3pcoR2[2WmIHHwc5B1d3Orc9Mg MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{NEi4PUc,OjR2MkS4PFk9N2F-
HPAEpiCs  M1zmNmZ2dmO2aX;uJGF{e2G7 MUKzNEDPxE1? MVWxJIg> MVLpcohq[mm2czDUUmYu|rFic4TpcZVt[XSnZDDwOFIweDR2IF3BVGsheGixc4Doc5J6dGG2aX;u NFrQTpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES0NVg4OCd-MkS0OFE5PzB:L3G+
BeWo MmHZSpVv[3Srb36gRZN{[Xl? NVTwRVFqOTEEoN88US=> Ml;qNkBp MofZbY5pcWKrdIOgSXJMOS9{ MnHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{O4OFYoRjJ2NEOzPFQ3RC:jPh?=
A498 NFnldHhCeG:ydH;zbZMhSXO|YYm= MVS1NOKh|ryP M1fsZ|I1KGh? MUPwc5RmdnSrYYTld:KhfGinIIDyc{1ieG:ydH;0bYMh\W[oZXP0d{Bw\iCQQx?= MnO4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEi0O|YoRjJ2NUC4OFc3RC:jPh?=
NHBE M{jmfmZ2dmO2aX;uJGF{e2G7 NE\4c3AzNzJyIN88US=> MlKwNkBp NWHqVmtC[XS2ZX71ZZRmeyCLTD2zN{B{fGmvdXzheIVlKEO[Q1y4M2lNNThic3XjdoV1cW:w MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5OUWyOkc,OjR2N{m1NlY9N2F-
A375 NXLXZYFrS2WubDDJcpZie2mxbjDBd5NigQ>? MnrMNVDjiJN{MDFCuW0> MU[yOEBp NHyxXWZz\WS3Y3XzJI1mdGGwb33hJINmdGxiaX72ZZNqd25? NGL1TWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OlA{Pid-MkS0OlYxOzZ:L3G+
HBMEC M33SVmZ2dmO2aX;uJGF{e2G7 MlXrNVAh|ryP M{fJclEhcA>? Mln5Zoxw[2u|IG\FS2YucW6mdXPl[EBGeGiDMjDlfJBz\XO|aX;u MmnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUi5PFIoRjJ2NEW4PVgzRC:jPh?=
HCT-15 M4\3fGZ2dmO2aX;uJGF{e2G7 NGPyZ5oyKGh? MVHheJRmdnWjdHXzJHBITTJvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiRYLrxsA> M2H5N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOxOFI2Lz5{NUSzNVQzPTxxYU6=
HCT-15 MkPaRZBweHSxc3nzJGF{e2G7 M2m0UVEhcA>? MUnhZo9tcXOqZYOgeIhmKHC{b4TlZ5RqfmViZX\m[YN1eyCxZjDQS2UzyqCjZ3HpcpN1KGO3cnP1cYlvNWmwZIXj[YQh[XCxcITvd4l{ NIXPN2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzNVQzPSd-MkW0N|E1OjV:L3G+
786-O MUnBdI9xfG:|aYOgRZN{[Xl? Ml2zOVDDqM7:TR?= NWDjeotLOjRiaB?= M{PtPZBwfGWwdHnheIV{yqC2aHWgdJJwNWGyb4D0c5Rq[yCnZn\lZ5R{KG:oIF7D Ml3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEi0O|YoRjJ2NUC4OFc3RC:jPh?=
HepG2  NHfwN2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVSyNEDPxE1? NHXwVJEzPCCq M4LXVZN2eHC{ZYPz[ZMhXEeILd8yNU1qdmS3Y3XkJINmdGxicILvcIln\XKjdHnvckBidmRiaX72ZZNqd25? MlXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NkC0PFgoRjJ3NU[wOFg5RC:jPh?=
SW480 M{T5bWZ2dmO2aX;uJGF{e2G7 M3q3cVIxyqEQvF2= M3HiSFHDqGh? NWfEdJN{emWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBCXEZ|IIDyc5RmcW5? NWrpW|lIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFc5OTZpPkK1OFQ4QDF4PD;hQi=>
MDA-MB-231 NWnBOlhITnWwY4Tpc44hSXO|YYm= NW\Pd4trOjVizszN MXWyMVMhcA>? MnTl[IVkemWjc3XzJJAuTVKNMT:yJIFv\CCVMUCwRVQh\XiycnXzd4lwdg>? NH7LNYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OVg4PSd-MkW1OVU5PzV:L3G+
HepG2  NEnqVHBHfW6ldHnvckBCe3OjeR?= MnS2NVAh|ryP NHLwdHY2KGh? NFzVXJBjdG:la4OgdIhwe3Cqb4L5cIF1\WRiTVHQT5MhcW6mdXPl[EBjgSCneH;n[Y5wfXNiVFfGMe6zOQ>? M3HL[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[wOFg5Lz5{NUW2NFQ5QDxxYU6=
MCF-7  MnK5SpVv[3Srb36gRZN{[Xl? M2PYN|ExKM7:TR?= NI\SPW8yKGh? NEjVWFlqdmirYnn0d{BKVC1zOD3lcohidmOnZDDj[YxtKG2rZ4LheIlwdg>? M2LhbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{K3NFEyLz5{NUeyO|AyOTxxYU6=
H1355 MXXGeY5kfGmxbjDBd5NigQ>? Mlz3NlUh|ryP MoPhNUBp NX\NSFdD[my3boTzJJRp\SCEW3HdVE1qdmS3Y3XkJIlv[3KnYYPlJIlvKHCqb4PwbI8uS2itMTDhcoQheGixc4Doc{1GWktiZYjwdoV{e2mxbh?= M3nGeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{[5NVgyLz5{NUe2PVE5OTxxYU6=
PC12 NVmxeZVXTnWwY4Tpc44h[XO|YYm= NHm1cINKdmirYnn0bY9vKG:oIH7ldpZmKGe{b4f0bEBn[WO2b4KtcYVlcWG2ZXSgUWFRKGurbnHz[UBi[3Srdnn0fUBqdiCqdX3hckBRSzF{IHPlcIx{NCCLQ{WwJF0hOiEQvF2u NFzpfpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC3O|M3Oyd-MUiwO|c{PjN:L3G+
HT-29 NVy0XIdySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MmjPOUBl[Xm| NIfafHhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IEWg[IF6eyCkeTDXV3QyKGG|c3H5MEBKSzVyIE2gOEDPxE1w NH7WPI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC3PFMyPid-MkWwO|g{OTZ:L3G+
IEC6 M4nXWGZ2dmO2aX;uJIF{e2G7 NES2eZc2KG2rboO= Mn[yTY5pcWKrdHnvckBw\iCPRVuxM|IhcW5icnH0JGlGSzZiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGVTUzFxMjDsc49xKHCqb4PwbI9zgWyjdHnvckBld3OnZDCzNEBucW6|IHLl[o9z\SC2bzDzeIlufWyjdHnvckB4cXSqIEGwKUB{\XK3bTDmc5IhPSCvaX7zJIJ6KGmvbYXuc4Jtd3S2aX7nJI1mfGixZDygTWM2OCB;IESuNkDPxE1w MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB5OEOxOkc,OjVyN{izNVY9N2F-
NG 108-15 MlP0SpVv[3Srb36gZZN{[Xl? NFTzSlREd26lZX70doF1cW:wIILldZVqemWmIITvJIFjd2yrc3igUWFRUyCjY4Tpeol1gSCrbjDtc5V{\SCwZYXyc4Jt[XO2b33hJIFv\CC{YYSg[4xqd22jIHj5ZpJq\CCQRzCxNFguOTViY3XscJMtKEGldHn2bZR6KD1iMUCg{txONg>? NET5e4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUWzO|M2PCd-MUW1N|c{PTR:L3G+
PC12 NWrHfG1DTnWwY4Tpc44h[XO|YYm= M1PLUFExKHWP MnjGOUBpenN? MkPvRYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCMTlugbY5pcWKrdH;yJHNRPjByMUK1JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN2NU[4OUc,OjF|NEW2PFU9N2F-
PC12 NF;4e4hHfW6ldHnvckBie3OjeR?= M3rReFExKHWP NIrsV4c2KGi{cx?= NEnGV3BC[3SrdnH0bY9vKG:oIF7y[lIwSVKHIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJGhQNTFicILveIVqdiCrbnT1Z5Rqd25iYYSgNVAhfU1iYX\0[ZIhPSCqcoOgdJJmfHKnYYTl[EB4cXSqIF3FT|IhcW6qaXLpeI9zKFVyMUK2JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NGP2eHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0OVY5PSd-MkGzOFU3QDV:L3G+
PC12 NVfwXWJYTnWwY4Tpc44h[XO|YYm= M3zk[|ExKHWP NG\KZVg2KGi{cx?= NGnQUIhC[3SrdnH0bY9vKG:oIF7y[lIwSVKHIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJGhQNTFicILveIVqdiCrbnT1Z5Rqd25iYYSgNVAhfU1iYX\0[ZIhPSCqcoOgdJJmfHKnYYTl[EB4cXSqIF3FT|EhcW6qaXLpeI9zKFCGOUiwOVkh\m:{IEGgbJIh[mWob4LlJINwdXCxdX7kJIFl\Gm2aX;uJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MonFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
HeLa M1u5UmZ2dmO2aX;uJIF{e2G7 MoXMNlAhfU1? MmTzN{BpenN? MlruTY5pcWKrdHnvckBw\iCHUluxM|IheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMkCgeW0h[W[2ZYKgN{BpenNiYomgW4V{fGW{bjDicI91fGmwZzDhcoFtgXOrcx?= NIG5fYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NFYyPSd-MkO1O|A3OTV:L3G+
RD NIPKUpVyUFSVIHHzd4F6 NV\ySWl7eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| NXfLdXAzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NXXheHFueUiWUzDhd5NigQ>? M4jpOJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= NX7TVJlVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NVLLOlZHeUiWUzDhd5NigQ>? M3;lfJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJFKGOnbHzz MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC NES2e3hyUFSVIHHzd4F6 NV\pPYNPeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NVn0XWtjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 MUjxTHRUKGG|c3H5 M4DXbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NWH3VmJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MDA-MB-231 Mn;pSpVv[3Srb36gZZN{[Xl? NF7EU5c2OCC3TR?= NF7q[XZFd3ewcnXneYxifGmxbjDv[kBOVVB{IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7Mk[yNlYoRjJ{OUK2NlI3RC:jPh?=
MDA-MB-231 MVvGeY5kfGmxbjDhd5NigQ>? MYS1NEB2VQ>? MVrEc5dvemWpdXzheIlwdiCxZjDNUXA6KGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7Mk[yNlYoRjJ{OUK2NlI3RC:jPh?=
Assay
Methods Test Index PMID
Western blot c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT ; p-JNK / JNK / Cyclin D1 ; p-HER2 / HER2 ; MMP9 / XIAP / VEGF 17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro MEK-inhibitory activity:

    Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.

Cell Research:

[1]

  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.

Animal Research:

[7]

  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

1mg/mL

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to formulate this inhibitor for i.p. injection?

Answer:
You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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