PD98059

For research use only.

Catalog No.S1177

343 publications

PD98059 Chemical Structure

CAS No. 167869-21-8

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

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Selleck's PD98059 has been cited by 343 publications

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Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  NF\NVm1HfW6ldHnvckBCe3OjeR?= NILM[nYzOMLizszN MkfJNUBp NIrZbmFjdG:la4OgSHRETC2rbnT1Z4VlKESUNTDlfJBz\XO|aX;u MoT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OU[4OlIoRjJ|Nkm2PFYzRC:jPh?=
AML 1# M4jxW2Fxd3C2b4Ppd{BCe3OjeR?= M{CwOFIxyqEQvF2= NW[1blY1OTJiaB?= MV;lcohidmOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGOx M3\r[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
KG-1  NE[3bZVCeG:ydH;zbZMhSXO|YYm= NEnxc24zOMLizszN MkXENVIhcA>? NHPBdFFmdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHMy NGjseXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewOlY6OSd-MkO3NFY3QTF:L3G+
HUVECs NIO2R25CeG:ydH;zbZMhSXO|YYm= M4fTUFIwPCEQvF2= NGjMN3AzPC92ODDo NXHPcGRycW6mdXPld{Bk\WyuIHTlZZRp MlXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEe1NlAoRjJ|N{C3OVIxRC:jPh?=
HepG2  M1KyPGZ2dmO2aX;uJGF{e2G7 MUCyNOKh|ryP NXz1endxOjRiaB?= MXfpcohq[mm2czD0bIUhUE9vMTDwdo91\WmwIHX4dJJme3Orb36gZ48ufHKnYYTl[EB4cXSqIH3leIZwem2rbh?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{[wPUc,OjN5MEe2NFk9N2F-
NB4  NUjxOWtESXCxcITvd4l{KEG|c3H5 M3jGNFExNzJyL{[wJO69VQ>? NHnTdXkyNjViaB?= NWLPTpluTE2VTx?= M{j0d4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDjc{11emWjdHXkJJdqfGhiUHHjcIl1[XinbB?= NGjaNYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ezOVU1OSd-MkO3N|U2PDF:L3G+
BxPC-3 cells M376Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfXblEzOMLizszN NHHycIIxNjViaB?= NH;3bmZqdmirYnn0d{BXTUeILVGtdoVofWyjdHXkJGhWXkWFIHfyc5d1cCCjbnSgeJVj\SCob4LtZZRqd25iaX7keYNm\CCkeTDQRXIuOiCDUB?= MoqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5NkSwOFYoRjJ|N{[0NFQ3RC:jPh?=
MKN45 M3T6W2Fxd3C2b4Ppd{BCe3OjeR?= NH7yd3oyOCEQvF5CpC=> NHPqTYczPCCq NUHLfJhGcW6lcnXhd4V{KHSqZTDERXBVNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= M2\iTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
SGC7901 MXvBdI9xfG:|aYOgRZN{[Xl? NH;ibZkyOCEQvF5CpC=> MYCyOEBp NIS0NVlqdmO{ZXHz[ZMhfGinIFTBVHQucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
MKN45 MlvpSpVv[3Srb36gRZN{[Xl? NELibWYyOCEQvF5CpC=> NUSyN4U1OjRiaB?= NEniVYRqdmirYnn0d{B1cGViZYjwdoV{e2mxbjDv[kBxcG:|cHjvdplt[XSnZDDFVmsyNzJ? MnjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
SGC7901 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWT5VIpEOTBizszNxsA> M1\GclI1NzR6L{eyJIg> NVzjOXBWcW6qaXLpeJMh[2WubDDndo94fGhiY3:teJJm[XSnZDD3bZRpKESDUGS= NU\NSI5HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
MKN45 M17nNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PhbVExKM7:TdMg MkfhNlQwPDhxN{KgbC=> MXfpcohq[mm2czDj[YxtKGe{b4f0bEBkdy22cnXheIVlKHerdHigSGFRXA>? NVnZVnI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 NHT6dIpHfW6ldHnvckBCe3OjeR?= MoHMNVAh|ryPwrC= M175d|I1KGh? NYfVelI3cW6qaXLpeJMhfGinIHX4dJJme3Orb36gc4YheGixc4Doc5J6dGG2ZXSgSXJMOS9{ NGG3ZoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
HUASMCs NELLZnRHfW6ldHnvckBCe3OjeR?= MWmxNEDPxE4EoB?= M3TCclI1KGh? NYXUVHlC\GmvaX7pd4hmeyCDbnegTWku[2G3c3XkJHNQS1N|IH3SUmEh[W6mIIDyc5RmcW5iZYjwdoV{e2mxbtMg NEn2R2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ixOlQ3QCd-MkO4NVY1Pjh:L3G+
HUASMCs MlTrSpVv[3Srb36gRZN{[Xl? NUXWUow2OTBizszNxsA> NHf5WIwzPCCq Ml\6bY5pcWKrdIOgRY5oKEmLLXnu[JVk\WRiRWLLNU8zKHCqb4PwbI9zgWyjdHnvckBt\X[nbB?= M3zoTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG2OFY5Lz5{M{ixOlQ3QDxxYU6=
EPOR/CR3 NXjtVZBvTnWwY4Tpc44hSXO|YYm= NF7L[2I2OMLizszN NFPrVXc{KGh? MknNdoVlfWOnczDFVG8h[W6mL3;yJGlNNTNvaX7keYNm\CC2aHWgeJlzd3OrbnWgdIhwe3Cqb4L5cIF1cW:wwrC= M3PPNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEKwO|MyLz5{M{iyNFc{OTxxYU6=
NB4  NXrlW3U5TnWwY4Tpc44hSXO|YYm= NV3OWmNXOTBizszNxsA> MlLoO|IhcA>? MUXEUXNQ NVXzbJFtcW6qaXLpeJMh\GG|YYTpcoljNWmwZIXj[YQhS0RzMXKg[ZhxemW|c3nvci=> NEf6cVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOVU5PSd-MkO4NlU2QDV:L3G+
HL60  MmTZSpVv[3Srb36gRZN{[Xl? M3HYVlIxyqEQvF2= NIjoNHY4OiCq Mn35SG1UVw>? M3S4ZolvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= NXG3OGlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlU2QDVpPkKzPFI2PTh3PD;hQi=>
HUVECs M1;JR2Z2dmO2aX;uJGF{e2G7 NV\udpc6OjVizszN MkTtNUBp NIq2bJNqdmO{ZXHz[UBPTi4QulKgdFY2KG63Y3zlZZIhfHKjboPsc4NifGmxbh?= NWrscnBlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFExODhpPkKzPVAyODB6PD;hQi=>
LNCaP  MoDDSpVv[3Srb36gRZN{[Xl? MYSxNEDPxE4EoB?= MoCyNUBp NF7UNZJl\WO{ZXHz[ZMhfGinIFXHSkB2eHKnZ4XsZZRm\CCyLWnCMVE> M3XET|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEO4N|E5Lz5{M{izPFMyQDxxYU6=
HL-60 MWPBdI9xfG:|aYOgRZN{[Xl? NILw[HQ2OMLizszN MmOxNUBp Mnn1doV{[3WnczDCRVE1PSCvZXTpZZRm\CCjcH;weI9{cXN? NHq3OWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0PFc2OSd-MkO5OFg4PTF:L3G+
HepG2 MXPGeY5kfGmxbjDBd5NigQ>? MmG1OFAh|ryP MVu2M|EzKGh? MlTnbY5pcWKrdIOgeIhmKGmwY4LlZZNmKG:oIICtSXJMOSCjbnSgdE1kNUq3bjDwdo91\WmwIHX4dJJme3Orb36gZpkhWEx? M3zqTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUSyPFUyLz5{M{m0Nlg2OTxxYU6=
HPAF-II NXLWRW5oTnWwY4Tpc44hSXO|YYm= MlL3NVAh|ryPwrC= MXO2JIg> MmmybY5kemWjc3WgcYlTNTF2MzDlfJBz\XO|aX;u M{fVdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
BxPC-3 Mn;6SpVv[3Srb36gRZN{[Xl? NXTGWm9tOTBizszNxsA> MmjJOkBp MUTpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= M3\2O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
COLO205 Mk\5RZBweHSxc3nzJGF{e2G7 NUCzPW5MOTBxMkCvOFAh|ryP M4TvTFI1KGh? MkDxbY5lfWOnczDEUmEhdGGmZHXyJIZwem2jdHnvci=> MknVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUmxNFgoRjJ2MEG5NVA5RC:jPh?=
G292  MVrBdI9xfG:|aYOgRZN{[Xl? Ml3UN|DDqM7:TR?= MVyyJIg> MorndoV{fG:{ZYOgZ4Fxe2GrY3nuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> M{KwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEGyPVMxLz5{NECxNlk{ODxxYU6=
SGC7901 NFXlT3RCeG:ydH;zbZMhSXO|YYm= NU\KPZpYOjEEoN88US=> NEXCNYgyKGh? MnfibY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhUU[QLd8xxsBidmRiNfMAtk1FTlWU MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB{N{e1NEc,OjRyMke3OVA9N2F-
MGC803  MWnBdI9xfG:|aYOgRZN{[Xl? NW\QeVdXOjEEoN88US=> NYCxdJQ4OSCq M4iwPYlvcGmkaYTzJIFxd3C2b4Ppd{BqdmS3Y3XkJIJ6KEmITj5OteKh[W6mIEZihNIuTE[XUh?= NGfPXow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyO|c2OCd-MkSwNlc4PTB:L3G+
HPMC M{XwPWFxd3C2b4Ppd{BCe3OjeR?= NV7XU|JzOTEEoN88US=> MmrqNlTDqGh? NV;PXGlkemW4ZYLz[ZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkhcW6mdXPl[EBjgSCKR2DEVy=> MkPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNEK4N|goRjJ2MESyPFM5RC:jPh?=
HCT-8 Mm\3RZBweHSxc3nzJGF{e2G7 NFi2ZWkyOMLizszN M{XrZlI1yqCq NIDheHRl\WO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUA2NU[X NYHtcoh7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVU5PjNpPkK0NFk2QDZ|PD;hQi=>
HPMC MYHGeY5kfGmxbjDBd5NigQ>? M3PjSVExyqEQvF2= MYK0PEBp MVTy[ZZmenOnczD0bIUh[2ijbnfld{BqdiClZXzsJI1wenCqb3zv[5khcW6mdXPl[EBjgSCKR2DEVy=> M3y4Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
A549 MmLwSpVv[3Srb36gRZN{[Xl? NFnMZmE2OMLizszN M1;4WlIhcA>? M1n0boJtd2OtczDFVmsheGixc4Doc5J6dGG2aX;uJI1m\GmjdHXkJIJ6KDFuMj3OVS=> NELSV3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2O|czPyd-MkSwOlc4Ojd:L3G+
Caco-2  NYLLVVBpSXCxcITvd4l{KEG|c3H5 NXrOOmRnOTEEoN88US=> MVyyOOKhcA>? NHTWW4tl\WO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUA2NU[X MlG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW4OlMoRjJ2MEm1PFY{RC:jPh?=
AGS NW[yXVNSTnWwY4Tpc44hSXO|YYm= M1O4[|ExKM7:TdMg NIi3doQxNjViaB?= M3HM[4lvcGmkaYTzJJRp\SC3cILl[5Vt[XSrb36gc4YhfGinIFnMMVgh\2WwZR?= MorFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzME[xOlYoRjJ2MUC2NVY3RC:jPh?=
Ca9-22 M3PMb2Z2dmO2aX;uJGF{e2G7 NV7zWXFFOyEQvF2= M1S4PFEwOiCq M1LLepJm\HWlZYOgTIJTNWmwZIXj[YQhSVSILUKgdIhwe3Cqb4L5cIF1cW:w NUPRWI06RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY2OzJpPkK0NVI3PTN{PD;hQi=>
Ca9-22 NY\mOIcxTnWwY4Tpc44hSXO|YYm= NYTlWnpWOyEQvF2= NIn4NpoyKGh? M2jHUYFjd2yrc3jld{B1cGViYXLpcIl1gSCxZjDIZnIhfG9iaX7keYNmKEmOLUigdJJw\HWldHnvci=> NFHBSFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOlU{Oid-MkSxNlY2OzJ:L3G+
HAECs MmnjSpVv[3Srb36gRZN{[Xl? M{jYOFExKM7:TR?= NV3qOXA2OSCq NWe4N3VWyqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= M4\CfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO0OlU4Lz5{NEGzOFY2PzxxYU6=
Caco-2 NWfhNndkTnWwY4Tpc44hSXO|YYm= NUHtRpBIPTEEoN88US=> NV35VppxPDiq MUfEUXNQ Mn[0[Y5p[W6lZYOgeIhmKG2UTlGgcIV3\Wy|IH;mxsBUS06QMVGsSnh[TDNuwrDMR3QtyqCOT2isxsBJUUZ|QT|CpHpIOTZuwrDQSGU3ScLiYX7kxsBNT0GOU{G2xsBo\W6nc9MgZ48ufHKnYYTl[EB4cXSqIFTlfC=> Ml\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkG2PVUoRjJ2MU[xOlk2RC:jPh?=
MCF-7 M4\TbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGxNEDPxE1? M4i4RlQ5cA>? MmjQSG1UVw>? MmnRdoV3\XK|ZYOgRm5HNWmwZIXj[YQh[2WubDDjfYNt\SCjcoLld5Q> MlPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkO0NFQoRjJ2MU[zOFA1RC:jPh?=
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Raji  NUfid5BUTnWwY4Tpc44hSXO|YYm= NXPEO25SOTBizszN M173NlEhcA>? MWPicI9kc3NiaIPCRWZHKGmwZIXj[YQhTXKtMT:yJJBpd3OyaH;yfYxifGmxbh?= NWLPeWV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3OzBpPkK0NlY6PjNyPD;hQi=>
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HEK 293 NECxWnpHfW6ldHnvckBCe3OjeR?= Mn\DNVAh|ryP MornOUBp NFniWI5FVVOR Mk\nd5VxeHKnc4Pld{B1cGViQ2LUJIFkfGm4aYT5 MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN{NEO2Okc,OjR|MkSzOlY9N2F-
HL-60  MYPGeY5kfGmxbjDBd5NigQ>? MXexNE8zOCEQvF2= NXjrO2lqOSCq NWLISI1TcW6qaXLpeJMhfGinwrDOMkBkcGmwZX7zbZNmgHS{YXP0xsBqdmS3Y3XkJIRq\m[ncnXueIlifGmxbjDpcpRwKGe{YX71cI9kgXSncx?= NFrBPGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO1O|AzOCd-MkSzOVcxOjB:L3G+
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HeLa NYPrdWRQTnWwY4Tpc44hSXO|YYm= NFW4dVY2OCEQvF2= NYTBS4VzOC53IHi= M4PJTYJtd2OtczDUVnguOSCwdXPs[YFzKG2rZ4LheIlwdiCjbnSgWHhPUVBiZH;3ck1z\We3bHH0bY9v M2HQeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2PFI4Lz5{NEO3OlgzPzxxYU6=
HUVECs NIS4RnBHfW6ldHnvckBCe3OjeR?= M4e2b|ExyqEQvF2= MX[xJIg> NV;oTnZ1cW6qaXLpeJMhfGinIFjEUEBz\WS3Y3XkJGNQYC1{IHX4dJJme3Orb36gZY5lKFCJST2yJJJmdGWjc3W= M33Eb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{i1NVA6Lz5{NEO4OVExQTxxYU6=
MCF-7 MnK3SpVv[3Srb36gRZN{[Xl? NHLNZpQyOMLizszN M1fnRVExNzNyIH3pci=> M{Pkd5Jm\HWlZYOgeIhmKFWWUD3k[ZBmdmSnboSgSXJMKHCqb4PwbI9zgWyjdHnvci=> MlvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|OUC4NVkoRjJ2M{mwPFE6RC:jPh?=
HGC-27 MWXBdI9xfG:|aYOgRZN{[Xl? NV7yUW5qOSEEtV2= NH3vOJgyKGh? MXXzeZBxemW|c3XzJHJCTDByMTDwcJV{KE2NLUKyNFYucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NVfLbZczRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NVY{PDlpPkK0OFE3OzR7PD;hQi=>
PC3  M3\VXWFxd3C2b4Ppd{BCe3OjeR?= NIXtNoQ2OCEQvF2= MkDnNE42KGh? NWfXfWpRcW6qaXLpeJMhVUi\LUS0PU1qdmS3Y3XkJIFxd3C2b4Ppd:Kh NF64b2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyOFg5QSd-MkS0NlQ5QDl:L3G+
HPAEpiCs  MX\GeY5kfGmxbjDBd5NigQ>? MoXxN|Ah|ryP M2LOVFEhcA>? M1fZU4lvcGmkaYTzJHRPTi4QsTDzeIlufWyjdHXkJJA1Oi:yNESgUWFRUyCyaH;zdIhwenmuYYTpc44> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2MUi3NEc,OjR2NEG4O|A9N2F-
BeWo NES5[nlHfW6ldHnvckBCe3OjeR?= NEjwO3gyOMLizszN MWqyJIg> NH\RO|hqdmirYnn0d{BGWktzL{K= M3L2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEOzPFQ3Lz5{NESzN|g1PjxxYU6=
A498 MmLsRZBweHSxc3nzJGF{e2G7 NHPFWGU2OMLizszN NXv1WGVnOjRiaB?= MV\wc5RmdnSrYYTld:KhfGinIIDyc{1ieG:ydH;0bYMh\W[oZXP0d{Bw\iCQQx?= MlfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEi0O|YoRjJ2NUC4OFc3RC:jPh?=
NHBE NF7WU2xHfW6ldHnvckBCe3OjeR?= NGHmSnIzNzJyIN88US=> MmexNkBp NUXYe4N6[XS2ZX71ZZRmeyCLTD2zN{B{fGmvdXzheIVlKEO[Q1y4M2lNNThic3XjdoV1cW:w MnixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m1NlYoRjJ2NEe5OVI3RC:jPh?=
A375 NVm1dHRXS2WubDDJcpZie2mxbjDBd5NigQ>? M17BO|Ex6oDVMkCgxtVO NGHi[nUzPCCq M4fvdZJm\HWlZYOgcYVt[W6xbXGgZ4VtdCCrbo\hd4lwdg>? NXXG[Vl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlYxOzZpPkK0OFY3ODN4PD;hQi=>
HBMEC NGrmWFlHfW6ldHnvckBCe3OjeR?= NWrCeGFYOTBizszN NG\3Z2wyKGh? MXficI9kc3NiVlXHSk1qdmS3Y3XkJGVxcEF{IHX4dJJme3Orb36= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3OEm4Nkc,OjR2NUi5PFI9N2F-
HCT-15 NWX3N3hCTnWwY4Tpc44hSXO|YYm= MV6xJIg> Mn\2ZZR1\W63YYTld{BRT0V{LXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb:Kh NHLpOWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzNVQzPSd-MkW0N|E1OjV:L3G+
HCT-15 NIOwTHhCeG:ydH;zbZMhSXO|YYm= M2rUb|EhcA>? MmjDZYJwdGm|aHXzJJRp\SCycn;0[YN1cX[nIHXm[oVkfHNib3[gVGdGOsLiYXfhbY5{fCCldYLjeY1qdi2rbnT1Z4VlKGGyb4D0c5Nqew>? M3nmR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOxOFI2Lz5{NUSzNVQzPTxxYU6=
786-O NUDzSHJKSXCxcITvd4l{KEG|c3H5 MX21NOKh|ryP Ml\iNlQhcA>? M1HlUZBwfGWwdHnheIV{yqC2aHWgdJJwNWGyb4D0c5Rq[yCnZn\lZ5R{KG:oIF7D M3G0NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUC4OFc3Lz5{NEWwPFQ4PjxxYU6=
HepG2  MnzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXyyNEDPxE1? NYn3NmR1OjRiaB?= NHvYb4J{fXCycnXzd4V{KFSJRj5OtlEucW6mdXPl[EBk\WyuIIDyc4xq\mW{YYTpc44h[W6mIHnueoF{cW:w Mm\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NkC0PFgoRjJ3NU[wOFg5RC:jPh?=
SW480 NUjvZXpmTnWwY4Tpc44hSXO|YYm= M3\ReVIxyqEQvF2= MYOxxsBp MkG3doVlfWOnczD0bIUh\XiycnXzd4lwdiCxZjDBWGY{KHC{b4TlbY4> NHHpRW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS0O|gyPid-MkW0OFc5OTZ:L3G+
MDA-MB-231 MmP3SpVv[3Srb36gRZN{[Xl? NFvBfVMzPSEQvF2= MkPnNk0{KGh? MYTk[YNz\WG|ZYOgdE1GWktzL{KgZY5lKFNzMEDBOEBmgHC{ZYPzbY9v MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NUi3OUc,OjV3NUW4O|U9N2F-
HepG2  NFTLZ3NHfW6ldHnvckBCe3OjeR?= NWq0V4NIOTBizszN M4PYdVUhcA>? M3zCbYJtd2OtczDwbI9{eGixconsZZRm\CCPQWDLd{BqdmS3Y3XkJIJ6KGW6b3flco92eyCWR1[t{tIy NEPueoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NFQ5QCd-MkW1OlA1QDh:L3G+
MCF-7  NUPQU|BzTnWwY4Tpc44hSXO|YYm= MlTINVAh|ryP NIHtVYcyKGh? NEHMOItqdmirYnn0d{BKVC1zOD3lcohidmOnZDDj[YxtKG2rZ4LheIlwdg>? NHXPOFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUeyO|AyOSd-MkW3NlcxOTF:L3G+
H1355 MXrGeY5kfGmxbjDBd5NigQ>? NWT3cI45OjVizszN NEfJWJUyKGh? M2fiNYJtfW62czD0bIUhSlujXWCtbY5lfWOnZDDpcoNz\WG|ZTDpckBxcG:|cHjvMWNpczFiYX7kJJBpd3OyaH:tSXJMKGW6cILld5Nqd25? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4OUG4NUc,OjV5NkmxPFE9N2F-
PC12 NIPJTpNHfW6ldHnvckBie3OjeR?= M1fFOGlvcGmkaYTpc44hd2ZibnXyeoUh\3Kxd4ToJIZi[3Sxcj3t[YRq[XSnZDDNRXAhc2mwYYPlJIFkfGm4aYT5JIlvKGi3bXHuJHBEOTJiY3XscJMtKEmFNUCgQUAzKM7:TT6= NH2wZ4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC3O|M3Oyd-MUiwO|c{PjN:L3G+
HT-29 NFn2TW5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWG1JIRigXN? M4DXVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWFEh[XO|YYmsJGlEPTBiPTC0JO69VS5? NYjH[2Q3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwO|g{OTZpPkK1NFc5OzF4PD;hQi=>
IEC6 NEfr[XZHfW6ldHnvckBie3OjeR?= M2SyN|UhdWmwcx?= MXzJcohq[mm2aX;uJI9nKE2HS{GvNkBqdiC{YYSgTWVEPiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gSXJMOS9{IHzvc5AheGixc4Doc5J6dGG2aX;uJIRwe2WmIEOwJI1qdnNiYnXmc5JmKHSxIIP0bY12dGG2aX;uJJdqfGhiMUClJJNmenWvIH\vdkA2KG2rboOgZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:mLDDJR|UxKD1iND6yJO69VS5? M3ixNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEe4N|E3Lz5{NUC3PFMyPjxxYU6=
NG 108-15 M2jiemZ2dmO2aX;uJIF{e2G7 M4fnOWNwdmOnboTyZZRqd25icnXxeYlz\WRidH:gZYJwdGm|aDDNRXBMKGGldHn2bZR6KGmwIH3veZNmKG6ndYLvZoxie3SxbXGgZY5lKHKjdDDncIlwdWFiaInidollKE6JIEGwPE0yPSClZXzsd{whSWO2aY\peJkhRSBzMDFOwG0v M{DlblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUO3N|U1Lz5zNUWzO|M2PDxxYU6=
PC12 NXLZcoJFTnWwY4Tpc44h[XO|YYm= NFzCVXkyOCC3TR?= NVmzRpZDPSCqcoO= Mny3RYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCMTlugbY5pcWKrdH;yJHNRPjByMUK1JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? Mnu4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
PC12 MnvESpVv[3Srb36gZZN{[Xl? M{O3ZVExKHWP MWC1JIhzew>? NGjkVXVC[3SrdnH0bY9vKG:oIF7y[lIwSVKHIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJGhQNTFicILveIVqdiCrbnT1Z5Rqd25iYYSgNVAhfU1iYX\0[ZIhPSCqcoOgdJJmfHKnYYTl[EB4cXSqIF3FT|IhcW6qaXLpeI9zKFVyMUK2JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MmW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
PC12 NVvGO29yTnWwY4Tpc44h[XO|YYm= NFPaZWoyOCC3TR?= M4DuXFUhcHK| NYDTWnM2SWO2aY\heIlwdiCxZjDOdoYzN0GURTDpckBz[XRiUFOxNkBk\WyuczDhd5Nme3OnZDDhd{BJVy1zIIDyc5RmcW5iaX7keYN1cW:wIHH0JFExKHWPIHHmeIVzKDViaILzJJBz\XS{ZXH0[YQhf2m2aDDNSWsyKGmwaHnibZRweiCSREm4NFU6KG[xcjCxJIhzKGKnZn;y[UBkd22yb4Xu[EBi\GSrdHnvckBjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NVLCOXJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzOFU3QDVpPkKxN|Q2Pjh3PD;hQi=>
HeLa MmKzSpVv[3Srb36gZZN{[Xl? MmfXNlAhfU1? M{jxc|MhcHK| NFXKdm5KdmirYnn0bY9vKG:oIFXST|EwOiCyaH;zdIhwenmuYYTpc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCyNEB2VSCjZoTldkA{KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nz NG\GXVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NFYyPSd-MkO1O|A3OTV:L3G+
RD MV7xTHRUKGG|c3H5 M{nXcpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>? Mnm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 NXnLS2tieUiWUzDhd5NigQ>? M{ezdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= M{KyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD NXPvOWZjeUiWUzDhd5NigQ>? MXzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucx?= NXnPWoc5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MYHxTHRUKGG|c3H5 M3;1WZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz NVfL[lF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 MYLxTHRUKGG|c3H5 NFvSOFRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ NY[4OFdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MDA-MB-231 MXfGeY5kfGmxbjDhd5NigQ>? NVrVZYliPTBidV2= NFLlNJNFd3ewcnXneYxifGmxbjDv[kBOVVB{IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MkX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7Mk[yNlYoRjJ{OUK2NlI3RC:jPh?=
MDA-MB-231 M1T3[WZ2dmO2aX;uJIF{e2G7 NEP0NFg2OCC3TR?= NIX5c2FFd3ewcnXneYxifGmxbjDv[kBOVVB7IHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOVAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl{NkKyOkc,OjJ7Mk[yNlY9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol

Kinase Assay:

[1]

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In vitro MEK-inhibitory activity:

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.
Cell Research:

[1]

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  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.


    (Only for Reference)
Animal Research:

[7]

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  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (52.37 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to formulate this inhibitor for i.p. injection?

  • Answer:

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID