PD98059

For research use only.

Catalog No.S1177

365 publications

PD98059 Chemical Structure

CAS No. 167869-21-8

PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.

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Selleck's PD98059 has been cited by 365 publications

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Biological Activity

Description PD98059 is a non-ATP competitive MEK inhibitor with IC50 of 2 μM in a cell-free assay, specifically inhibits MEK-1-mediated activation of MAPK; does not directly inhibit ERK1 or ERK2. PD98059 is a ligand for the aryl hydrocarbon receptor (AHR) and functions as an AHR antagonist.
Features Does not inhibit c-Raf phosphorylated MEK1.
Targets
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
In vitro

PD98059 inhibits either basal MEK1 or a partially activated MEK produced by mutation of serine at residues 218 and 222 to glutamate (MEK-2E) with IC50 of 2 μM. PD98059 does not inhibit the MAPK homologues JNK and P38. PD98059 is highly selective against MEK, as it does not inhibit a number of other kinase activities including Raf kinase, cAMP-dependent kinase, protein kinase C, v-Src, epidermal growth factor (EGF) receptor kinase, insulin receptor kinase, PDGF receptor kinase, and phosphatidylinositol 3-kinase. PD98059 inhibits PDGF-stimulated activation of MAPK and thymidine incorporation into 3T3 cells with IC50 of ~10 μM and ~7 μM, respectively. [1] PD98059 potently prevents the activation of MEK1 by Raf or MEK kinase with IC50 of 4 μM, and weakly inhibits the activation of MEK2 by Raf with IC50 of 50 μM. PD98059 does not inhibit the activation of MEK homologues MKK4 and RK kinase that participate in stress and interleukin-1-stimulated kinase cascades in KB and PC12 cells, and the activation of p70 S6 kinase by insulin or epidermal growth factor in Swiss 3T3 cells. [2] PD98059 completely blocks the nerve growth factor (NGF)-induced differentiation of PC12 cells without altering cell viability. [3] PD98059 inhibits the proliferation of RAW264.7 cells in the culture containing RANKL in a dose-dependent manner, resulting in an apparent decrease of TRAP-positive cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  NHfpSmdHfW6ldHnvckBCe3OjeR?= MUiyNOKh|ryP MX[xJIg> NHnC[o9jdG:la4OgSHRETC2rbnT1Z4VlKESUNTDlfJBz\XO|aX;u MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ7Nki2Nkc,OjN4OU[4OlI9N2F-
AML 1# NX3CS3lRSXCxcITvd4l{KEG|c3H5 MmDVNlDDqM7:TR?= M33VS|EzKGh? MX;lcohidmOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGOx NYD0OVQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFY3QTFpPkKzO|A3PjlzPD;hQi=>
KG-1  NVvJPHY3SXCxcITvd4l{KEG|c3H5 NHey[WEzOMLizszN NIf4eXQyOiCq NF65boxmdmijbnPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHMy M2O1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
HUVECs NVLBTHdmSXCxcITvd4l{KEG|c3H5 MlXDNk81KM7:TR?= Mo[wNlQwPDhiaB?= NYrQR2tZcW6mdXPld{Bk\WyuIHTlZZRp M3HyZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3OVIxLz5{M{ewO|UzODxxYU6=
HepG2  MY\GeY5kfGmxbjDBd5NigQ>? MniwNlDDqM7:TR?= M1HTSVI1KGh? MY\pcohq[mm2czD0bIUhUE9vMTDwdo91\WmwIHX4dJJme3Orb36gZ48ufHKnYYTl[EB4cXSqIH3leIZwem2rbh?= M4DJTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3OlA6Lz5{M{ewO|YxQTxxYU6=
NB4  NH;5ToRCeG:ydH;zbZMhSXO|YYm= MofCNVAwOjBxNkCg{txO NWDpfJplOS53IHi= M2jzdWROW09? MW\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSnZDD3bZRpKFCjY3zpeIF5\Wx? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NUW0NUc,OjN5M{W1OFE9N2F-
BxPC-3 cells MkXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLsNlDDqM7:TR?= NFPNTWoxNjViaB?= MYPpcohq[mm2czDWSWdHNUFvcnXneYxifGWmIFjVWmVEKGe{b4f0bEBidmRidIXi[UBnd3KvYYTpc44hcW6mdXPl[EBjgSCSQWKtNkBCWA>? NXX3XYM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OlQxPDZpPkKzO|Y1ODR4PD;hQi=>
MKN45 M1TYPWFxd3C2b4Ppd{BCe3OjeR?= M4Pv[lExKM7:TdMg M1\HVFI1KGh? MWHpcoNz\WG|ZYOgeIhmKESDUGStbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? NWPGOW5RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 NYq2SWg6SXCxcITvd4l{KEG|c3H5 NI\0OoIyOCEQvF5CpC=> NXnYb4Q2OjRiaB?= MkTRbY5kemWjc3XzJJRp\SCGQWDUMYlv\HWlZXSgZ4VtdCCjcH;weI9{cXN? M4XEOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
MKN45 NGrGfWlHfW6ldHnvckBCe3OjeR?= NFq4UGMyOCEQvF5CpC=> MXWyOEBp NHLtU5RqdmirYnn0d{B1cGViZYjwdoV{e2mxbjDv[kBxcG:|cHjvdplt[XSnZDDFVmsyNzJ? NXm4PYR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVI2QDhpPkKzO|kzPTh6PD;hQi=>
SGC7901 NETuR5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPZPVkyOCEQvF5CpC=> MoH4NlQwPDhxN{KgbC=> M{HuR4lvcGmkaYTzJINmdGxiZ4Lve5RpKGOxLYTy[YF1\WRid3n0bEBFSVCW M3ztb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
MKN45 MkDMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zVelExKM7:TdMg NG\Fco8zPC92OD:3NkBp NHy5U21qdmirYnn0d{Bk\WyuIHfyc5d1cCClbz30doVifGWmIIfpeIghTEGSVB?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 NYXpN2hnTnWwY4Tpc44hSXO|YYm= NVP2VlFsOTBizszNxsA> M{\QSlI1KGh? M1zaS4lvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIFXST|EwOg>? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
HUASMCs NWrHXFhtTnWwY4Tpc44hSXO|YYm= M1[5Z|ExKM7:TdMg NUTQO3NROjRiaB?= MVXkbY1qdmm|aHXzJGFv\yCLST3jZZV{\WRiU1;DV|MhdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vyqB? MnHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MU[0OlgoRjJ|OEG2OFY5RC:jPh?=
HUASMCs NGP1eFFHfW6ldHnvckBCe3OjeR?= Mny0NVAh|ryPwrC= NITRZWYzPCCq MVjpcohq[mm2czDBcochUUlvaX7keYNm\CCHUluxM|IheGixc4Doc5J6dGG2aX;uJIxmfmWu Mn;WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MU[0OlgoRjJ|OEG2OFY5RC:jPh?=
EPOR/CR3 MXXGeY5kfGmxbjDBd5NigQ>? M3fHXVUxyqEQvF2= M1\5cVMhcA>? MXzy[YR2[2W|IFXQU{BidmRxb4KgTWwuOy2rbnT1Z4VlKHSqZTD0fZJwe2mwZTDwbI9{eGixconsZZRqd28EoB?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEezNUc,OjN6MkC3N|E9N2F-
NB4  MkXpSpVv[3Srb36gRZN{[Xl? NYHCSnBzOTBizszNxsA> M4LVV|czKGh? Mof5SG1UVw>? M1L5ZolvcGmkaYTzJIRie2G2aX7pZk1qdmS3Y3XkJGNFOTGkIHX4dJJme3Orb36= M3XvclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK1OVg2Lz5{M{iyOVU5PTxxYU6=
HL60  NFKyNmVHfW6ldHnvckBCe3OjeR?= MWKyNOKh|ryP M2XkSVczKGh? MYXEUXNQ MnTDbY5pcWKrdIOg[IF{[XSrbnniMYlv\HWlZXSgR2QyOWJiZYjwdoV{e2mxbh?= NVTP[phkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlU2QDVpPkKzPFI2PTh3PD;hQi=>
HUVECs NHToXWpHfW6ldHnvckBCe3OjeR?= MortNlUh|ryP MnHSNUBp NFXsNGhqdmO{ZXHz[UBPTi4QulKgdFY2KG63Y3zlZZIhfHKjboPsc4NifGmxbh?= NGrvOIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwNVAxQCd-MkO5NFExODh:L3G+
LNCaP  MXHGeY5kfGmxbjDBd5NigQ>? NIDBRVcyOCEQvF5CpC=> MV2xJIg> M2PsWIRm[3KnYYPld{B1cGViRVfGJJVxemWpdXzheIVlKHBvWVKtNS=> M3zvXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEO4N|E5Lz5{M{izPFMyQDxxYU6=
HL-60 M4fmU2Fxd3C2b4Ppd{BCe3OjeR?= M4j0VlUxyqEQvF2= MVqxJIg> NHjMSoxz\XOldXXzJGJCOTR3IH3l[IlifGWmIHHwc5B1d3Orcx?= M1q4SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUS4O|UyLz5{M{m0PFc2OTxxYU6=
HepG2 MY\GeY5kfGmxbjDBd5NigQ>? M3m0UVQxKM7:TR?= NETKNZk3NzF{IHi= MYLpcohq[mm2czD0bIUhcW6lcnXhd4Uhd2ZicD3FVmsyKGGwZDDwMYMuUnWwIIDyc5RmcW5iZYjwdoV{e2mxbjDifUBRVA>? MljmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NEK4OVEoRjJ|OUSyPFUyRC:jPh?=
HPAF-II NH7Fbo1HfW6ldHnvckBCe3OjeR?= NFHsO3MyOCEQvF5CpC=> Mlq1OkBp M13BWIlv[3KnYYPlJI1qWi1zNEOg[ZhxemW|c3nvci=> M4\UeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
BxPC-3 NICwUJVHfW6ldHnvckBCe3OjeR?= Mn7ENVAh|ryPwrC= MlfpOkBp MX\pcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= MmOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O3NVAoRjJ|OUezO|ExRC:jPh?=
COLO205 NEfCd3VCeG:ydH;zbZMhSXO|YYm= MVixNE8zOC92MDFOwG0> NX3iW4RxOjRiaB?= MnHybY5lfWOnczDEUmEhdGGmZHXyJIZwem2jdHnvci=> NX3a[JUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkyODhpPkK0NFE6OTB6PD;hQi=>
G292  M3i1S2Fxd3C2b4Ppd{BCe3OjeR?= MojzN|DDqM7:TR?= MYiyJIg> NETqVJhz\XO2b4Lld{Bk[XC|YXnjbY4ucW6mdXPl[EBk\WyuIHTlZZRp MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzMkmzNEc,OjRyMUK5N|A9N2F-
SGC7901 NVHHfWI3SXCxcITvd4l{KEG|c3H5 M{nGe|IxyqEQvF2= MYmxJIg> NVz0Wmc5cW6qaXLpeJMh[XCxcITvd4l{KGmwZIXj[YQh[nliSV\OMe6yyqCjbnSgOgKBui2GRmXS M{nC[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEK3O|UxLz5{NECyO|c2ODxxYU6=
MGC803  M1u1NmFxd3C2b4Ppd{BCe3OjeR?= M2LUeFIxyqEQvF2= NWP0Z4lmOSCq Ml6ybY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhUU[QLd8xxsBidmRiNfMAtk1FTlWU NILOOZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyO|c2OCd-MkSwNlc4PTB:L3G+
HPMC NVG5eJI6SXCxcITvd4l{KEG|c3H5 NE[1NVEyOMLizszN MmfmNlTDqGh? NFTE[XVz\X[ncoPld{Bl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KEiJUFTT MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB2MkizPEc,OjRyNEK4N|g9N2F-
HCT-8 NEDsZ5BCeG:ydH;zbZMhSXO|YYm= M4\QXVExyqEQvF2= NEP2bWkzPMLiaB?= M2S2eIRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? NHT5NW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVg3Oyd-MkSwPVU5PjN:L3G+
HPMC NH;vNpZHfW6ldHnvckBCe3OjeR?= MojsNVDDqM7:TR?= NEDne2Q1QCCq MVTy[ZZmenOnczD0bIUh[2ijbnfld{BqdiClZXzsJI1wenCqb3zv[5khcW6mdXPl[EBjgSCKR2DEVy=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB2MkizPEc,OjRyNEK4N|g9N2F-
A549 NV[2ZZdyTnWwY4Tpc44hSXO|YYm= MnHjOVDDqM7:TR?= NVrTSHdwOiCq MUTicI9kc3NiRWLLJJBpd3OyaH;yfYxifGmxbjDt[YRq[XSnZDDifUAyNDJvTmG= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB4N{eyO{c,OjRyNke3Nlc9N2F-
Caco-2  Mn\WRZBweHSxc3nzJGF{e2G7 M2KzV|ExyqEQvF2= NYPQb3h2OjUEoHi= MVjk[YNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTC1MWZW Ml\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUW4OlMoRjJ2MEm1PFY{RC:jPh?=
AGS MVPGeY5kfGmxbjDBd5NigQ>? NVjuPGdXOTBizszNxsA> NVvxNpk2OC53IHi= MkPobY5pcWKrdIOgeIhmKHWycnXneYxifGmxbjDv[kB1cGViSVytPEBo\W6n MlzhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzME[xOlYoRjJ2MUC2NVY3RC:jPh?=
Ca9-22 MmXaSpVv[3Srb36gRZN{[Xl? M2jzPFMh|ryP M2\scVEwOiCq MVzy[YR2[2W|IFjiVk1qdmS3Y3XkJGFVTi1{IIDoc5NxcG:{eXzheIlwdg>? MlPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[1N|IoRjJ2MUK2OVMzRC:jPh?=
Ca9-22 MnK3SpVv[3Srb36gRZN{[Xl? NEOyTnQ{KM7:TR?= MVyxJIg> M3K1fYFjd2yrc3jld{B1cGViYXLpcIl1gSCxZjDIZnIhfG9iaX7keYNmKEmOLUigdJJw\HWldHnvci=> NUfpV4Y{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY2OzJpPkK0NVI3PTN{PD;hQi=>
HAECs NYrTbWlNTnWwY4Tpc44hSXO|YYm= NVziXmNwOTBizszN MWqxJIg> NX3UNmtjyqCjdITlcpVifGW|IGTOSk3PuS2|dHnteYxifGWmIFnDRW0uOSCjbnSgWmNCVS1zIHX4dJJme3Orb36= NEPWRYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGzOFY2Pyd-MkSxN|Q3PTd:L3G+
Caco-2 NH\UTYlHfW6ldHnvckBCe3OjeR?= M3j4[lUxyqEQvF2= NIjLfYc1QGh? NXG0flZFTE2VTx?= NFLXeIJmdmijbnPld{B1cGVibWLORUBt\X[nbIOgc4bDqFOFTl6xRUxHYFmGMz|CpGxEXC{EoFzPXEzDqEiLRkPBMOKhYkdzNj|CpHBFTT[DwrDhcoTDqEyJQVzTNVbDqGenbnXzxsBkdy22cnXheIVlKHerdHigSIV5 NVTaXWR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOlE3QTVpPkK0NVYyPjl3PD;hQi=>
MCF-7 NX3mUlZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYGxNEDPxE1? M1jjRlQ5cA>? MnuySG1UVw>? MnrDdoV3\XK|ZYOgRm5HNWmwZIXj[YQh[2WubDDjfYNt\SCjcoLld5Q> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF4M{SwOEc,OjRzNkO0NFQ9N2F-
SMMC7721 NY\kU4ZETnWwY4Tpc44hSXO|YYm= MmDyNlUwPTBizszN NYDFU5lvOjRiaB?= MXLzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJJAuSWu2IH;yJJAuTVKNMT:yxsA> M4nFWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[4NFU3Lz5{NEG2PFA2PjxxYU6=
SGC7901  M{W2VmFxd3C2b4Ppd{BCe3OjeR?= MVy1NOKh|ryP M4PQRVI1NzR6L{eyJIg> M1TrXmROW09? MlW4bY5lfWOnczDhdI9xfG:|aYOgZ49u[mmwZXSge4l1cCCMQVuyJJNpWk6D NVLrRpNKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|gzPDBpPkK0NVc5OjRyPD;hQi=>
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DLD-1  M2naXWZ2dmO2aX;uJGF{e2G7 Ml\tNlDDqM7:TR?= MXW0PEBp M4DqbZJm\HWlZYOgeIhmKEKQSWCzJIV5eHKnc4Ppc44heHKnLYTy[YF1\WRid3n0bEA2NWG8YT3kRy=> NGDzSoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVU5OSd-MkSyNVE2QDF:L3G+
HT-29 MmTWSpVv[3Srb36gRZN{[Xl? NFHZUFIzOMLizszN NYHTR4N7PDhiaB?= NXnKOZgxemWmdXPld{B1cGViQl7JVFMh\XiycnXzd4lwdiCycnWteJJm[XSnZDD3bZRpKDVvYYrhMYRE MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUW4NUc,OjR{MUG1PFE9N2F-
7402 NVzLUVlCSXCxcITvd4l{KEG|c3H5 MkmyN|Ah|ryP MYO1JIQ> MYHk[YNz\WG|ZYOgZ4VtdCCycn;sbYZmemG2aX;u M{\XWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxNlU{Lz5{NEKxNVI2OzxxYU6=
MCF-7 NGTt[IZHfW6ldHnvckBCe3OjeR?= NFezWW4zOCEQvF2= NF3sTnYyKGh? Mn\0ZYJwdGm|aHXzJIV5eHKnc4Ppc44hd2ZicHjvd5Bpd3K7bHH0[YQhTVKNIHPvMZRz\WG2bXXueEB4cXSqIHPvcop2\2G2ZR?= MnnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MU[yPFkoRjJ2MkG2Nlg6RC:jPh?=
MCF-7 MnnLRZBweHSxc3nzJGF{e2G7 MlHnNlAh|ryP NWXxSoRkOSCq M4ixdYlv[3KnYYPld{Bk[XOyYYPlMVkh\W68eX3lJIFkfGm4aYT5 NVT4[Y1WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVYzQDlpPkK0NlE3Ojh7PD;hQi=>
CRL-2302 NGDSflBHfW6ldHnvckBCe3OjeR?= NIPrVIszOCEQvF2= NXfzc|NXOC53IHi= MmS2bY5pcWKrdIOgRZBmdGmwLXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb{BidmRiQXv0 MmPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{Mke5NVgoRjJ2MkK3PVE5RC:jPh?=
ARPE-19 M3exS2Z2dmO2aX;uJGF{e2G7 NYPkOJFROjBizszN MYmwMlUhcA>? MXHpcohq[mm2czDBdIVtcW5vaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiRYLrJIFv\CCDa4S= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{N{mxPEc,OjR{Mke5NVg9N2F-
MG-63 MXTGeY5kfGmxbjDBd5NigQ>? MVKyNEDPxE1? MVywMlUhcA>? M2PFeIJtd2OtczD0bIUhS0hvaX7keYNm\CCyaH;zdIhwenmuYYTl[EBGVEtzIIDyc5RmcW5iZYjwdoV{e2mxbh?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ|OU[0NEc,OjR{M{m2OFA9N2F-
SGC-7901  NFnV[olCeG:ydH;zbZMhSXO|YYm= MVqyNEDPxE1? NGf2OGszPCCq M3jwVIlvcGmkaYTzJGNRNW2nZHnheIVlKGGyb4D0c5Nqew>? MmDDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NEGzOVEoRjJ2MkSxN|UyRC:jPh?=
NUGC2 MlnMSpVv[3Srb36gRZN{[Xl? MXe1NEDPxE1? NIDoPZY1QCCq NI\BTXdFVVOR MWPpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
NUGC3 MVfGeY5kfGmxbjDBd5NigQ>? MnPKOVAh|ryP MlWwOFghcA>? Mn[zSG1UVw>? MYXpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| NXnHVW5ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
KATOIII  MoHTSpVv[3Srb36gRZN{[Xl? NYL5S2hEPTBizszN NYrKWJVLPDhiaB?= NUn2TI95TE2VTx?= NGK2eIhqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZjDIUGEuSTB{IH;yJGhNSS2DMkSgcY9t\WO3bHXz NVzXbpY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
NCI-N87  MVTGeY5kfGmxbjDBd5NigQ>? NEHXfIc2OCEQvF2= MVi0PEBp MV3EUXNQ MXjpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
OE19 Ml;aSpVv[3Srb36gRZN{[Xl? M2jE[|UxKM7:TR?= NFPBTlk1QCCq MoTXSG1UVw>? NUTyTYtxcW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> M4\aUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
MKN7 NVfmXmdMTnWwY4Tpc44hSXO|YYm= MWK1NEDPxE1? MmHDOFghcA>? NHjkXVhFVVOR MYTpcoNz\WG|ZYOg[ZhxemW|c3nvckBw\iCKTFGtRVAzKG:{IFjMRU1COjRibX;s[YN2dGW| NUHScXNORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE4 M3nVWmZ2dmO2aX;uJGF{e2G7 MWS1NEDPxE1? MoTWOFghcA>? NX;VXZBZTE2VTx?= MV;1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBJVEFiY3zhd5MhUQ>? M1rGPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
KYSE30 MXLGeY5kfGmxbjDBd5NigQ>? M2i1VlUxKM7:TR?= NWXSRYNZPDhiaB?= NHrafIlFVVOR M{nCdZVxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKEiOQTDjcIF{eyCL NWnocpVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE5 MnXWSpVv[3Srb36gRZN{[Xl? NIfYdHc2OCEQvF2= MnXMOFghcA>? NHrpc3JFVVOR NWLPN2h1fXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFzBJINt[XO|IFm= M4\nZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
TE1 MlrtSpVv[3Srb36gRZN{[Xl? MYS1NEDPxE1? MmDtOFghcA>? Ml[2SG1UVw>? NWfpW2p7fXC{ZXf1cIF1\XNidHjlJIV5eHKnc4Ppc44hd2ZiSFzBJINt[XO|IFm= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
TE3 NFTsOplHfW6ldHnvckBCe3OjeR?= MnLxOVAh|ryP MWG0PEBp Mn7MSG1UVw>? Mk\3eZBz\We3bHH0[ZMhfGinIHX4dJJme3Orb36gc4YhUEyDIHPsZZN{KEl? NULXOW9jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
KYSE30 NFXMVGRHfW6ldHnvckBCe3OjeR?= NIT1TGUzOC93MD:xNFAh|ryP MkiyOFghcA>? NV7RXYxpTE2VTx?= M4rGUIlvcGmkaYTzJJAuTXKtIHHu[EB4d3K2bXHucolvKGSxd37y[Yd2dGG2ZXSgdE1Cc3RiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MlPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
TE1 MnTHSpVv[3Srb36gRZN{[Xl? NVzV[HAxOjBxNUCvNVAxKM7:TR?= NUTxNFFIPDhiaB?= MWHEUXNQ M{LBdolvcGmkaYTzJJAuTXKtIHHu[EB4d3K2bXHucolvKGSxd37y[Yd2dGG2ZXSgdE1Cc3RiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NHnrWYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
TE4 MlW5SpVv[3Srb36gRZN{[Xl? MWmyNE82OC9zMECg{txO NHzUb201QCCq MXXEUXNQ MkfabY5pcWKrdIOgdE1GemtiYX7kJJdwenSvYX7ubY4h\G:5boLl[5Vt[XSnZDDwMWFsfCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NFTRcJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
HT29 M4TwWWZ2dmO2aX;uJGF{e2G7 NFHBTlcyOCEQvF2= Ml;yNkBp NGDoUFdqdmirYnn0d{Bw\iCMQVuyMEBGWktzL{KgZY5lKFOWQWSzJJBpd3OyaH;yfYxifGmxbh?= M3;kNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[1Nlk{Lz5{NEK2OVI6OzxxYU6=
HepG2 M4L0cWFxd3C2b4Ppd{BCe3OjeR?= M{C0NlIxKM7:TR?= Mn\jNlQhcA>? NUSwWXF{cW6qaXLpeJMhTVKNMT:yJJBpd3OyaH;yfYxifGmxbjDhcoQh\W6qYX7j[ZMhXkJzLXnu[JVk\WRiYYDvdJRwe2m| NXTSfW4yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFc6ODlpPkK0NlQ4QTB7PD;hQi=>
HepG2 NIHxfG9HfW6ldHnvckBCe3OjeR?= MlTQNlAh|ryP M2XUcFIhcA>? M1frd4VvcGGwY3XzJHZDOS2rbnT1Z4VlKE[RWF:zZUB1emGwc3PybZB1cW:wYXygZYN1cX[rdIm= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2N{mwPUc,OjR{NEe5NFk9N2F-
MC-3 MYTBdI9xfG:|aYOgRZN{[Xl? MYexNEDPxE1? MYCyOEBp MXPwc5RmdnSrYYTl[EBOTVOFLXnu[JVk\WRiYYDvdJRwe2m|IHnuJEBk\Wyucx?= NH3KUYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK3NFUzOyd-MkSyO|A2OjN:L3G+
Raji  Mne1SpVv[3Srb36gRZN{[Xl? MV[xNEDPxE1? MUGxJIg> NUnUeVlR[myxY3vzJIh{SkGIRjDpcoR2[2WmIFXyb|EwOiCyaH;zdIhwenmuYYTpc44> NE\jdmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVY{OCd-MkSyOlk3OzB:L3G+
Raji  MlLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\hOZcyOCEQvF2= MWWxJIg> MnfFbY5pcWKrdIOgeIhmKGKjc3HsJI9zKGi|QlHGSk1{fGmvdXzheIVlKGOnbHygdJJwdGmoZYLheIlwdiCjbnSgeoli[mmuaYT5 M2T5SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[5OlMxLz5{NEK2PVY{ODxxYU6=
A549 M2jjfGZ2dmO2aX;uJGF{e2G7 NWf3cGl1OzBizszN Mm[3NE42KGh? Mlf5SG1UVw>? M2SxdYlvcGmkaYTzJJRpem:vYnnuMYlv\HWlZXSgR{9GSlEQsjDUbJIzOzYEoIDoc5NxcG:{eXzheIlwdg>? NYD0ZnZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyO|c3QTZpPkK0Nlc4Pjl4PD;hQi=>
HCSMCs NID5O3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVWxNEDPxE1? M{jaXlI1KGh? M{TEd4Jtd2OtczDGRWJRPC2rbnT1Z4VlKEiFQWPNR{Bxem:uaX\ldoF1cW:w M{W2fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{GyN|gyLz5{NEOxNlM5OTxxYU6=
PANC-1 M2SwSmZ2dmO2aX;uJGF{e2G7 MlH4NlAh|ryP NVX1WGxSPDhiaB?= MmiwbY5pcWKrdIOgeIhmKGW6cILld5Nqd25ib3[g{rQ3TCCrbjDy[ZNxd26|ZTD0c{B1cGViUGDBVu61yqCjZ3;ubZN1yqB? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7NEGzN{c,OjR{OUSxN|M9N2F-
A549 MmjSSpVv[3Srb36gRZN{[Xl? M2jLRlMxKM7:TR?= M3TZbFAvPSCq MlvBSG1UVw>? MkLDbY5pcWKrdIOgeIhmKHSqcn;tZolvNWmwZIXj[YQhUUxvOD;DXGNNQC2OdXOgZYN1cX[rdIm= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5N{[5Okc,OjR{N{e2PVY9N2F-
SW480 M{ixTmZ2dmO2aX;uJGF{e2G7 M3fPcVExKM7:TR?= M2CwN|IxKGh? M2j2NmROW09? MlvQd5VxeHKnc4Pld{B1cGViQ2LUJIFkfGm4aYT5 M4\iZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 M{PiUGZ2dmO2aX;uJGF{e2G7 MXixNEDPxE1? NHzWd4I2KGh? M1\zWmROW09? MoKxbY5pcWKrdIOgW451NWmwZIXj[YQh|rJvY3H0[Y5qdi:WQ1[0JIFkfGm4aYT5JIFv\CCwdXPs[YFzKM7{LXPheIVvcW5iYXPjeY12dGG2aX;u NXPvZVg6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlQ{PjZpPkK0N|I1OzZ4PD;hQi=>
HEK 293 MWjGeY5kfGmxbjDBd5NigQ>? NVnD[HRPOTBizszN MXu1JIg> MnLWSG1UVw>? MVfzeZBxemW|c3XzJJRp\SCFUmSgZYN1cX[rdIm= M4\SclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
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HBMEC NF\WbmVHfW6ldHnvckBCe3OjeR?= MlfaNVAh|ryP Ml;UNUBp MYricI9kc3NiVlXHSk1qdmS3Y3XkJGVxcEF{IHX4dJJme3Orb36= NFzBW2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES1PFk5Oid-MkS0OVg6QDJ:L3G+
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HepG2  Moq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXwdowzOCEQvF2= MXuyOEBp MXjzeZBxemW|c3XzJHRITi4QskGtbY5lfWOnZDDj[YxtKHC{b3zp[oVz[XSrb36gZY5lKGmwdnHzbY9v NFnpZWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NFQ5QCd-MkW1OlA1QDh:L3G+
SW480 MX;GeY5kfGmxbjDBd5NigQ>? MlLBNlDDqM7:TR?= MlrJNeKhcA>? NGfuV2Vz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mJGFVTjNicILveIVqdg>? NVvIVm9nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFc5OTZpPkK1OFQ4QDF4PD;hQi=>
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HepG2  MnP0SpVv[3Srb36gRZN{[Xl? M{DIelExKM7:TR?= Mmf4OUBp M4rzVIJtd2OtczDwbI9{eGixconsZZRm\CCPQWDLd{BqdmS3Y3XkJIJ6KGW6b3flco92eyCWR1[t{tIy M4fBR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[wOFg5Lz5{NUW2NFQ5QDxxYU6=
MCF-7  NIO1U|ZHfW6ldHnvckBCe3OjeR?= MmrZNVAh|ryP Mlq1NUBp MkDSbY5pcWKrdIOgTWwuOThvZX7oZY5k\WRiY3XscEBucWe{YYTpc44> M4XVTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{K3NFEyLz5{NUeyO|AyOTxxYU6=
H1355 MnLpSpVv[3Srb36gRZN{[Xl? MYiyOUDPxE1? MmjjNUBp MVzicJVvfHNidHjlJGJc[V2SLXnu[JVk\WRiaX7jdoVie2ViaX6gdIhwe3Cqbz3DbIsyKGGwZDDwbI9{eGixLVXST{BmgHC{ZYPzbY9v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4OUG4NUc,OjV5NkmxPFE9N2F-
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TC32 MYjxTHRUKGG|c3H5 MWDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzz NF;jWJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MDA-MB-231 MUfGeY5kfGmxbjDhd5NigQ>? NX3MdHpvPTBidV2= NVXHbphwTG:5boLl[5Vt[XSrb36gc4YhVU2SMjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFUxKHWPIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NIrseJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+
MDA-MB-231 MYjGeY5kfGmxbjDhd5NigQ>? MYe1NEB2VQ>? NV22RVZXTG:5boLl[5Vt[XSrb36gc4YhVU2SOTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFUxKHWPIHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NFzYN4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
In vivo

Treatment of mice 30 minutes before focal cerebral ischemia with PD98059 protects against damage, resulting in a decrease in infarct volume. [5] Pretreated with PD98059 (10 mg/kg per i.v. injection) 30 minutes before and then together with hourly cerulein injections for 3 hours significantly ameliorates cerulein-induced acute pancreatitis ipancreatitis on the basis of pancreatic wet weight and histology. [6] Administration of PD98059 (10 mg/kg) in mice 1 hour after carrageenan causes a reduction in all the parameters of inflammation measured. [7]

Protocol

Kinase Assay:

[1]

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In vitro MEK-inhibitory activity:

Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50 μL of 50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE.
Cell Research:

[1]

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  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, and K562
  • Concentrations: Dissolved in DMSO, final concentrations ~100 μM
  • Incubation Time: 3 dyas, or 7-10 days
  • Method:

    For monolayer growth, cells are plated into multi-well plates at 10,000-20,000/mL. Forty-eight hours later, various concentrations of PD98059 are added to the cell growth medium and incubation is continued for an additional 3 days. Cells are then removed from the wells by incubation with trypsin and enumerated with a Coulter Counter. For growth in soft agar, cells are seeded into 35-mm dishes at 5,000-10,000 cells per dish with growth medium containing 0.3% agar and desired concentrations of PD98059. After 7-10 days of growth, visible colonies are manually enumerated with the aid of a dissecting microscope.


    (Only for Reference)
Animal Research:

[7]

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  • Animal Models: Male Sprague–Dawley rats with acute pancreatitis
  • Dosages: 10 mg/kg
  • Administration: Injection i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (52.37 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.28
Formula

C16H13NO3

CAS No. 167869-21-8
Storage powder
in solvent
Synonyms N/A
Smiles COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    How to formulate this inhibitor for i.p. injection?

  • Answer:

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID