PD184352 (CI-1040)

Catalog No.S1020

For research use only.

PD184352 (CI-1040) is an ATP non-competitive MEK1/2 inhibitor with IC50 of 17 nM in cell-based assays, 100-fold more selective for MEK1/2 than MEK5. PD184352 (CI-1040) selectively induces apoptosis. Phase 2.

PD184352 (CI-1040) Chemical Structure

CAS No. 212631-79-3

Selleck's PD184352 (CI-1040) has been cited by 123 publications

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Biological Activity

Description PD184352 (CI-1040) is an ATP non-competitive MEK1/2 inhibitor with IC50 of 17 nM in cell-based assays, 100-fold more selective for MEK1/2 than MEK5. PD184352 (CI-1040) selectively induces apoptosis. Phase 2.
Features First MEK inhibitor to begin clinical development.
Targets
MEK1 [1]
(Cell-free assay)
MEK2 [1]
(Cell-free assay)
17 nM 17 nM
In vitro

CI-1040 treatment produces a reduction of pMAPK levels in multiple tumor cells including Colon 26, BX-PC3 pancreatic, A431 cervical, HT-29 colon, ZR-25-1 breast and SKOV-3 ovarian carcinomas. CI-1040 treatment doesn't inhibit the phosphorylation of Jun kinase, p38 kinase or Akt, indicating CI-1040 specifically targets MEK. Inhibition of MAPK activation by CI-1040 prevents cell cycle progression and induces a G1 block. [1] The IC50 for inhibition of MEK1 by CI-1040 is 0.3 μM, 15-fold higher than the concentration required to inhibit the EGF-induced activation of ERK2 in Swiss 3T3 cells. These results indicate CI-1040 exerts its effects on cells by suppressing the activation of MKK1, and not by blocking its activity. 2 nM PD184352 inhibits the activation of MKK1 in Swiss 3T3 cells by 50%, while over 100-fold concentration of CI-1040 inhibits MEK1 in vitro. PD184352 also inhibits the Raf-catalysed phosphorylation of MEK1 without any effect on the Raf-catalysed phosphorylation of myelin basic protein. [2] CI-1040 inhibits 86% of papillary thyroid carcinoma (PTC) cell growth with the RET/PTC1 rearrangement at 10μM compared with cells treated with DMSO only. CI-1040 shows potent inhibition to PTC cells (BRAF mutation) with GI50 of 52 nM, but low activity to RET/PTC1 rearrangement type with GI50 of 1.1 μM. [3] A recent research indicates CI-1040 increases the apoptotic effect of BMS-214662 in a CML blast crisis cell line, K562, and in primary chronic phase CD34+ CML cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC-3 M{P6Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTJ2LkC1NlYh|ryP Ml7qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
VM-CUB-1 MnLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTJ|LkezOFEh|ryP M1zSN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
LC-2-ad MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1yzVmlEPTB;MkOuOlE1PyEQvF2= MmD5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
A704 M{LZNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTJ|Lk[xNFMh|ryP M1e4eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
DMS-114 M4\STGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;QN49FUUN3ME2yN{42PzZ3IN88US=> NH3TSlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
EKVX M4HOXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDjNm9KSzVyPUKzMlM6ODlizszN MlHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SNU-449 M3foXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjQTWM2OD1{Mz6zNlkh|ryP NFvvbGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
HCC1806 MoLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XYR2lEPTB;MkOuNFU5OyEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
CaR-1 NWDUPZBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{KxO2lEPTB;MkOuNFIyOyEQvF2= M{XxTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H441 M{\Dc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTJ{LkW2PFkh|ryP NFX4R3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
TI-73 NVz5ZnZDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmC1TWM2OD1{Mj6zNlgh|ryP NFnHOpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H1755 M1;3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYKwUWsxUUN3ME2yNU43PzRzIN88US=> MnzCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
A498 NIf3TlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXlT3BbUUN3ME2yNU42OzF{IN88US=> M{e0blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CFPAC-1 NFvjV4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLlTWM2OD1{MT6zPFU5KM7:TR?= NFzNXmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
ChaGo-K-1 NHjmXVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvldYk3UUN3ME2yNU4{PjJ5IN88US=> M4m4e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
KP-4 M1HNfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjwTWM2OD1{MT6xNVk3KM7:TR?= M4KxTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
Becker NVXjUVhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTJzLkGxNVgh|ryP Mlr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
UMC-11 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWD1bnNJUUN3ME2yNE46OTZ2IN88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
TE-6 MlLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTJyLkiwOlch|ryP M2izV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SF539 MnjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;JNWk3UUN3ME2yNE42OTd{IN88US=> MlvQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
FTC-133 NIrDfINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLiVXJkUUN3ME2yNE41PzB|IN88US=> MmjTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H226 NIXp[nBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWj2ZmlkUUN3ME2yNE41Ozl7IN88US=> Mnf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H1048 MkXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTJyLkOyOlYh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
Calu-3 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDyTWM2OD1zOT60NlM4KM7:TR?= M3jMWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SF126 MoXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTF7LkO1NFIh|ryP NEfpflg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NTERA-S-cl-D1 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXlcFlkUUN3ME2xPU4zODB5IN88US=> NWTvPYkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SW1573 NGfYWmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{K1[mlEPTB;MUmuNVk3QSEQvF2= NXm0O293RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SNU-387 Ml2xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTF7LkG4PFkh|ryP NHPtU4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
ETK-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFiybVNKSzVyPUG5MlE5OjdizszN M4PJSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HuO9 MkmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTF7LkGzO|kh|ryP M3jueVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
RO82-W-1 MmLXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnMcHVKSzVyPUG4MlgzQDhizszN MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
D-502MG MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHk[YhKSzVyPUG4MlgyOzlizszN NHLhS2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
LS-123 NIe5PXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;xcmpnUUN3ME2xPE44QTBzIN88US=> NXr6bXA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
OC-314 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTF6LkezPFQh|ryP NGD3WHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
Ramos-2G6-4C10 NX7ZfYtsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTJTWM2OD1zOD61PVI4KM7:TR?= NHfMWGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
SW962 NXLkNIdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vMTmlEPTB;MUiuOVcxOiEQvF2= M{\5PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
SW982 NWTOVpl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTF6LkW2OFkh|ryP MmXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
KYSE-410 NUfu[5BNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXaT3l3UUN3ME2xPE41PTd5IN88US=> M2f1cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HT-3 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTF6LkO5O|ch|ryP NV3sWZg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
CAL-85-1 MlS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHqSFdKSzVyPUG4MlE5ODlizszN MlvNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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NCI-H1563 NH;SPWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DkZWlEPTB;MUWuN|Q6KM7:TR?= NFLX[2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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EC-GI-10 NFzofmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX30UmMzUUN3ME2xOU4zOzV5IN88US=> NHr2UZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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OVCAR-4 NEfyVGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHH1VY9KSzVyPUG1MlE1OjRizszN NFvhUIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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MKN7 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3DTWM2OD1zNT6wNFk{KM7:TR?= MknJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H661 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTF2Lkm1PUDPxE1? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LB831-BLC MkjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrSTWM2OD1zND64PVM1KM7:TR?= NX\QeWh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
BT-549 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TtS2lEPTB;MUSuPFI5PCEQvF2= Mk\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H2347 MmTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXftemxEUUN3ME2xOE45OjNizszN NF\yfY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
OVCAR-8 MkjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTF2LkeyNFEh|ryP NY\RUIhJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NCI-H630 NH\Kfm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTF2Lk[1Olgh|ryP Ml3HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
U-118-MG Mn34S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfQTFhKSzVyPUG0MlY{OSEQvF2= MnfRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
LS-1034 NFfCWoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlK4TWM2OD1zND62NVUzKM7:TR?= NGS5UHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
BALL-1 NFGxUJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHPc4tKSzVyPUG0MlQ3PjVizszN NFfoOpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
Capan-2 MnvRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrq[GFKSzVyPUG0MlM4OTRizszN MoiwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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HC-1 NGf1c4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGiyV5ZKSzVyPUG0MlIxQDdizszN NXXoR4pORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NCI-H2030 M3rI[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTF2LkG0OlUh|ryP NEDLNFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NB17 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzBfFRKSzVyPUG0MlA5PSEQvF2= NFT6SFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
GCT MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlezTWM2OD1zMz65Olk4KM7:TR?= NEfYZVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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GCIY NHLwOHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTF|Lk[5Nlch|ryP MlnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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NCI-H2009 NVnYW3hFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUC1Z4piUUN3ME2xN{42QTF7IN88US=> NGi0eIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
CAPAN-1 MoHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTF|LkW1NVQh|ryP NGXEUWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H2452 NV\HR2VkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3sVZFKSzVyPUGzMlIyPzVizszN NU\4SVl5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
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T-24 NFTOcpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfwV5hKSzVyPUKuPVEyOTJizszN MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
CAL-39 M1SxV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Tu[mlEPTB;Mj64PVk6QSEQvF2= NWDEdZdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
LB2241-RCC MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTTfphKSzVyPUKuPFU5QTNizszN NHfCW4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
COLO-792 M1HPNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnqUoxKSzVyPUKuPFQxOjdizszN MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
HCE-T NIPDfmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjKeYRKSzVyPUKuPFMzPjRizszN NWHTN5R1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
BFTC-905 NGDMdVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUK2d3hNUUN3ME2yMlgzPTNizszN M2rPfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CHL-1 NITzWIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTJwOEC3PFUh|ryP NV7RW2tZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
L-363 NE\HdYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIqzWWJKSzVyPUKuO|Q{PyEQvF2= M2LpelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HCC2218 NFX5[mJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrHVIliUUN3ME2yMlY1Pzd6IN88US=> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
DB NUPCOFVCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGe2XZVKSzVyPUKuOVg4QDhizszN MmrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
EW-13 NFzUfFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfCd|VGUUN3ME2yMlU3OzN7IN88US=> M3LiNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
KNS-62 NWX5XW1qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonzTWM2OD1{LkWxNVY1KM7:TR?= M2DPbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
RD M4nNd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTJwNES3NlMh|ryP NIP6PYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
BB65-RCC NGHX[|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPaTWM2OD1{LkSwOlc1KM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
CAL-27 MnzVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTJwM{myN|Yh|ryP NF7VTno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
DOK NEf0dpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTJwM{W3O|Mh|ryP MmS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NB7 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTJwM{SwPFYh|ryP M1zxW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
A2058 MmHPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn:0TWM2OD1{LkG5Nlc6KM7:TR?= NWPM[|ZTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
HN M1\m[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVP2OFk2UUN3ME2yMlE3Ojd6IN88US=> NWDPU416RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
MZ2-MEL MkLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\YbVJtUUN3ME2yMlEyOTJ5IN88US=> NYe3ZpJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
EM-2 M{fIR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFHsU2JKSzVyPUKuNFcyQThizszN M1u4[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HCC70 NGjL[JdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1u0OGlEPTB;Mj6wNlQzPiEQvF2= NFnsfHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
RXF393 NYLqWVQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDweXhDUUN3ME2xMlk4QDh5IN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LCLC-97TM1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2q1[mlEPTB;MT65NlI2PiEQvF2= M3zkXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
A375 M1zQ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrBOpJjUUN3ME2xMlg6PzF2IN88US=> NH7nbW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
BPH-1 M4nRcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLvTWM2OD1zLki5OVkyKM7:TR?= M2G1dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
LOXIMVI NHi4NmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHGTWM2OD1zLki5OFI4KM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NCI-H292 NXHFcZdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2X5SmlEPTB;MT64OlY6QSEQvF2= NGLXO409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NB69 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTFwN{i0Olkh|ryP MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
OVCAR-5 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFT1ZopKSzVyPUGuO|c3QDdizszN NYe3eJFPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
COLO-829 M13O[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DMSWlEPTB;MT62NlI{OiEQvF2= M1PVS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
RT-112 M4DYdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTUelhYUUN3ME2xMlU5QTV{IN88US=> NXzwTFhuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SK-MEL-2 NF\5[nJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTFwNUe0OVkh|ryP NHjvcII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
HD-MY-Z NYD0SWlQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTFwNUezO|Yh|ryP NWrOeIVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
A4-Fuk MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnZSZlHUUN3ME2xMlU4ODR3IN88US=> Mn\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SW1116 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;UV5NKSzVyPUGuOVY{QThizszN NGDKTHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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ML-2 NVrxcpR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;LTWM2OD1zLkWyPFY5KM7:TR?= MmfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
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LS-513 NYXsU|NbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TmdWlEPTB;MT60OVE6OiEQvF2= MnXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
BV-173 NWHyW4VJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvDVJlKSzVyPUGuOFQxOTVizszN NYCyV3I4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
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SW1463 M{HiU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1naXWlEPTB;MT6zN|czOSEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
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NB1643 MlH1dWhVWyCjc4PhfS=> NI\KTpRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz NF7Leow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 NVrweph5eUiWUzDhd5NigQ>? Ml3PdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NF;admc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 M4H4VJFJXFNiYYPzZZk> MkjpdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy| NIDPdIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 M{LqNpFJXFNiYYPzZZk> MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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MG 63 (6-TG R) MXzxTHRUKGG|c3H5 MYXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? NHewOnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
786-0 NEnWXIJHfW6ldHnvckBie3OjeR?= M3zvT|I1KGi{cx?= MnrzTY5pcWKrdHnvckBw\iCFS{KgbY4hcHWvYX6gO|g3NTBiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gRYt1OSCyaH;zdIhwenmuYYTpc44h[XRiU3XyNVI6KGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNuIFXDOVA:OC5zzszN MnXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4OEm5OFYoRjNyNki5PVQ3RC:jPh?=
Assay
Methods Test Index PMID
Western blot p-ERK1/2 / ERK1/2 ; p-ERK5 / ERK5 21762482
Growth inhibition assay Cell viability 29989268
In vivo Oral dosing of CI-1040 impairs the growth of colon tumor xenografts of mouse and human with a wide dose range of 48-200 mg/kg per dose, but not of P388 leukemia. [1] CI-1040 inhibits the tumor xenografts from PTC cells carrying a BRAF mutation with 31.3% reduction, carrying the RET/PTC1 rearrangement with 47.5% reduction than in untreated (vehicle) mice after 3 weeks of oral administration (300 mg/kg/d). No toxic effects are observed in any mice when they are treated with CI-1040. [2] Transient exposure of mammary tumors to CI-1040 and UCN-01 causes tumor cell death in vivo and prolonged suppression of tumor regrowth. Combined treatment with CI-1040 (25 mg/kg) and UCN-01 (0.1-0.2 mg/kg) significantly reduces MDA-MB-231, and largely abolishs MCF7 tumor growth in implanted athymic mice, while either single treatment has no significant activity. The drug combination leads to profound tumor cell death which correlates with a reduction in the phosphorylation of ERK1/2 and the immuno-reactivity of Ki67 and of CD31. [5]

Protocol (from reference)

Kinase Assay:

[2]

  • MEK1 Assay:

    MAP kinase is activated after phosphorylation by MEK; the activated MAP kinase subsequently phosphorylates myelin basic protein (MBP).Incorporation of 32P into myelin basic protein (MBP) is assayed in the presence of glutathione S-transferase (GST) fusion proteins containing the 44-kDa MAPK (GST-MAPK) or the 45-kDa MEK (GST-MEK1). Assays are conducted in 50μL of 50 mM Tris, pH 7.4/10 mM MgCl2 /2 mM EGTA/10 μM [γ-32P]ATP containing 10 μg of GST-MEK1, 0.5 μg of GST-MAPK, and 40 μg of MBP. After incubation at 30°C for 15 minutes, reactions are stopped by addition of Laemmli SDS sample buffer. Phosphorylated MBP is resolved by SDS/10% PAGE. This screening effort leads to the discovery of several small-molecule inhibitors of MEK, i.e. CI-1040. Experiments assessing the order of addition shows that CI-1040 directly inhibits MEK1 with a 50% inhibitory concentration (IC50) of 17 nM, without affecting the activity of MAPK.

Cell Research:

[1]

  • Cell lines: Colon 26 carcinoma cells
  • Concentrations: 0.1-10 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are planted seeded in T-75 cm 2 flasks and treated the next day for 24 hous with either DMSO or CI-1040. Single-cell suspensions are collected, and pellets are fixed in ice-cold ethanol (70%) for 30 minutes. After centrifugation of the samples, propidium iodide (50 μg/mL) and RNase (30 units/mL) are added to the pellets for 20 minutes at 37 °C. After filtration, samples are analyzed by flow cytometry.

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: PTC cells in athymic mice
  • Dosages: 150 mg/kg
  • Administration: Orally twice daily
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 96 mg/mL
(200.55 mM)
Ethanol 14 mg/mL
(29.24 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol, pH 9
For best results, use promptly after mixing.

10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 478.67
Formula

C17H14ClF2IN2O2

CAS No. 212631-79-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CC1CONC(=O)C2=C(C(=C(C=C2)F)F)NC3=C(C=C(C=C3)I)Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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