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Diethylstilbestrol Estrogen/progestogen Receptor agonist

Name: Diethylstilbestrol
Brand: Selleck Chemicals
SKU: S1859
Availability: InStock
Rating: 5 (11 reviews)

Cat.No.S1859

Diethylstilbestrol (Stilbestrol) is a synthetic nonsteroidal estrogen, used to prevent miscarriage and other pregnancy complications.This compound can be used to induce animal models of Pituitary Adenoma.

Chemical Structure

Molecular Weight: 268.35

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Quality Control

Batch: Purity: 99.92%

99.92

Related Targets	Adrenergic Receptor GPR Androgen Receptor Glucocorticoid Receptor ACE RAAS Progesterone Receptor Opioid Receptor PGES THR
Other Estrogen/progestogen Receptor Inhibitors	Elacestrant (RAD1901) Dihydrochloride MPP dihydrochloride Cholesterol Endoxifen HCl G15 Chrysin Licochalcone A AZD9496 PHTPP Liquiritigenin

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (372.64 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	268.35	Formula	C₁₈H₂₀O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	56-53-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Stilbestrol			Smiles	CCC(=C(CC)C1=CC=C(C=C1)O)C2=CC=C(C=C2)O

Mechanism of Action

Targets/IC₅₀/Ki	Estrogen receptor
In vitro	Diethylstilbestrol promotes coactivator release from orphan nuclear receptor ERR beta and inhibits its transcriptional activity in trophoblast stem cells.
In vivo	Diethylstilbestrol treated pregnant mice exhibits abnormal early placenta development associated with an overabundance of trophoblast giant cells and an absence of diploid trophoblast. This compound causes epigenetic methylation changes that result in persistent alterations in gene expression, leading to tumorigenesis in mouse uterus. It induces c-fos exon-4 hypomethylation at postnatal day 17 in mice uterus, while elevating its mRNA level from postnatal day 5. This chemical exposure leads to hypomethylation in the exon-4 region of c-fos mRNA. It-treated rats exhibits more pronounced delay in maturational development of an adult pattern of immunoexpression of the three proteins compared with GnRHa-treated rats. This compound results in similar reductions in both Sertoli cell numbers and suppression of testicular growth at 18 and 25 days, though by 35 days the suppression is more pronounced in DES-treated rats. This treatment appears to imprint an abnormal, site-specific demethylation of CpG/-464, which requires ovarian hormones to occur in adult mice. It (2.0 ng/g) per day increases adult prostate weight, whereas a 200 ng/g dose decreases adult prostate weight in male offspring mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/11297507/ [2] https://pubmed.ncbi.nlm.nih.gov/14502647/ [3] https://pubmed.ncbi.nlm.nih.gov/9780313/ [4] https://pubmed.ncbi.nlm.nih.gov/9331098/ [5] https://pubmed.ncbi.nlm.nih.gov/9050904/

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Handling Instructions

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