research use only

TPH2 Antibody [B20J2]

Cat.No.: F3163

Application: Reactivity: Human, Rat, Mouse

Lane 1: Mouse midbrain, Lane 2: Rat midbrain

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:2000 IF1:500 FCM1:500

Application
WB, IP, IHC, IF, FCM

Reactivity
Human, Rat, Mouse

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
56 kDa

Datasheet & SDS

Biological Description

Specificity
TPH2 Antibody [B20J2] detects endogenous levels of total TPH2 protein.

Clone
B20J2

Synonym(s)
NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

Background
Tryptophan hydroxylase 2 (TPH2), a brain-specific, rate-limiting enzyme catalyzing the conversion of L-tryptophan to 5-hydroxytryptophan (5-HTP) using tetrahydrobiopterin (BH4), O2, and ferrous iron as cofactors in the first committed step of serotonin (5-HT) biosynthesis within serotonergic neurons of raphe nuclei, features a conserved modular structure with an N-terminal regulatory domain (~100 residues) harboring autoinhibitory helices and phosphorylation sites (e.g., Ser19 by CaMKII, Ser260 by ERK for activation), a central catalytic domain with the iron-binding motif (H306NIT309 for Fe2+ coordination) and pterin-binding pocket (Trp²⁵⁹, Phe²⁶⁰ stabilizing BH4), plus a C-terminal tetramerization domain (Leu³⁶⁰-Leu⁴⁴⁰) forming homodimers or heterotetramers with TPH1 to enhance stability and activity. Activation occurs via N-terminal dephosphorylation/tryptophan binding relieving autoinhibition, BH4/Fe2+ saturation, and posttranslational modifications boosting Vmax up to 10-fold, feeding 5-HTP to aromatic L-amino acid decarboxylase (AADC) for 5-HT production that modulates synaptic transmission through G-protein-coupled receptors (5-HT1-7) regulating mood, anxiety, sleep, appetite, and cognition via downstream cAMP/PKA, PLCβ/IP3, and β-arrestin pathways, while TPH2 knockout mice exhibit 60-80% reduced brain 5-HT levels causing growth retardation, hyperactivity, impulsivity, and impaired fear conditioning alongside HPA axis dysregulation; polymorphisms like rs4570625 or Pro447Arg diminish enzyme activity linking to major depression, bipolar disorder, ADHD, and suicidality through blunted 5-HT tone and gene-environment interactions exacerbating stress vulnerability.

Background

Tryptophan hydroxylase 2 (TPH2), a brain-specific, rate-limiting enzyme catalyzing the conversion of L-tryptophan to 5-hydroxytryptophan (5-HTP) using tetrahydrobiopterin (BH4), O2, and ferrous iron as cofactors in the first committed step of serotonin (5-HT) biosynthesis within serotonergic neurons of raphe nuclei, features a conserved modular structure with an N-terminal regulatory domain (~100 residues) harboring autoinhibitory helices and phosphorylation sites (e.g., Ser19 by CaMKII, Ser260 by ERK for activation), a central catalytic domain with the iron-binding motif (H306NIT309 for Fe2+ coordination) and pterin-binding pocket (Trp²⁵⁹, Phe²⁶⁰ stabilizing BH4), plus a C-terminal tetramerization domain (Leu³⁶⁰-Leu⁴⁴⁰) forming homodimers or heterotetramers with TPH1 to enhance stability and activity. Activation occurs via N-terminal dephosphorylation/tryptophan binding relieving autoinhibition, BH4/Fe2+ saturation, and posttranslational modifications boosting Vmax up to 10-fold, feeding 5-HTP to aromatic L-amino acid decarboxylase (AADC) for 5-HT production that modulates synaptic transmission through G-protein-coupled receptors (5-HT1-7) regulating mood, anxiety, sleep, appetite, and cognition via downstream cAMP/PKA, PLCβ/IP3, and β-arrestin pathways, while TPH2 knockout mice exhibit 60-80% reduced brain 5-HT levels causing growth retardation, hyperactivity, impulsivity, and impaired fear conditioning alongside HPA axis dysregulation; polymorphisms like rs4570625 or Pro447Arg diminish enzyme activity linking to major depression, bipolar disorder, ADHD, and suicidality through blunted 5-HT tone and gene-environment interactions exacerbating stress vulnerability.

References
https://pubmed.ncbi.nlm.nih.gov/36046836/ https://pubmed.ncbi.nlm.nih.gov/32119710/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%