KDM1/LSD1 (Nuclear Marker) Antibody [P12K1]

Catalog No.: F3362

    Home Primary Antibodies KDM1/LSD1 (Nuclear Marker) Antibody [P12K1]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:10000 - 1:50000
    1:10 - 1:100
    1:50 - 1:250
    1:450
    1:20
    5 µg for 25 µg chromatin
    Application
    WB,IP, IHC, IF, FCM, ChIP
    Reactivity
    Mouse, Rat, Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    92 kDa, 155 kDa 110 kDa, 160 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    KDM1/LSD1 (Nuclear Marker) Antibody [P12K1] detects endogenous levels of total KDM1/LSD1 protein.
    Clone
    P12K1
    Synonym(s)
    AOF2; KDM1; KIAA0601; LSD1; KDM1A; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2; [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A
    Background
    KDM1A, also known as lysine-specific demethylase 1 (LSD1), is a flavin adenine dinucleotide (FAD)-dependent histone demethylase belonging to the amine oxidase family, playing a pivotal role in epigenetic regulation. LSD1 catalyzes the removal of mono- and di-methyl groups from lysine residues on histone H3, primarily H3K4me1/2 and H3K9me1/2, thereby functioning as a transcriptional co-repressor or co-activator depending on cellular context. LSD1 features three conserved domains: an N-terminal SWIRM domain that mediates chromatin targeting and protein-protein interactions; a central, LSD1-specific Tower domain that facilitates binding to the CoREST corepressor complex; and a C-terminal amine oxidase domain containing the catalytic site for FAD-dependent oxidative demethylation. The enzymatic reaction involves hydride transfer and the formation of an imine intermediate to excise methyl groups, with cofactors like CoREST required for efficient nucleosomal demethylation. LSD1 regulates gene expression programs involved in hematopoiesis, embryogenesis, and neuronal differentiation. It also demethylates non-histone proteins such as p53 and DNMT1, influencing cellular proliferation, DNA repair, and epigenetic stability. Additionally, LSD1 contributes to epithelial-to-mesenchymal transition (EMT) by repressing epithelial markers and is implicated in cancers. LSD1 is localized in the nucleus, serving as a nuclear marker fundamental to chromatin remodeling and transcriptional control.
    References
    • https://pubmed.ncbi.nlm.nih.gov/27479862/
    • https://pubmed.ncbi.nlm.nih.gov/36188536/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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