Citrate synthetase Antibody [K19C21]

Catalog No.: F3776

    Home Primary Antibodies Citrate synthetase Antibody [K19C21]

    For research use only.

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000 - 1:10000
    1:250 - 1:500
    1:100 - 1:500
    1:20
    Application
    WB, IHC, IF, FCM
    Reactivity
    Mouse, Rat, Human
    Source
    Rabbit Monoclonal Antibody
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    51 kDa,73 kDa 45 kDa,78 kDa, 51 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    Citrate synthetase Antibody [K19C21] detects endogenous levels of total Citrate synthetase protein.
    Clone
    K19C21
    Synonym(s)
    Citrate (Si)-synthase; CS
    Background
    Citrate synthase (CS) is a mitochondrial enzyme classified within the citrate synthase family, playing a crucial role in cellular metabolism as it catalyzes the first and rate-limiting step of the tricarboxylic acid (TCA) cycle, or Krebs cycle. Citrate synthase is a globular protein of approximately 433 to 437 amino acids organized mainly into two alpha-helical domains separated by a cleft that forms the enzyme’s active site. Key catalytic residues include histidine and aspartate residues (His274, His320, Asp375), which facilitate substrate interaction and catalysis. The enzyme undergoes a significant conformational change upon substrate binding, allowing oxaloacetate to bind first and induce a closed conformation that creates the binding site for acetyl-CoA, ensuring proper catalysis and prevention of premature hydrolysis. Citrate synthase catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and coenzyme A in an aldol-Claisen condensation followed by hydrolysis, making it pivotal for energy production through ATP generation by mitochondrial respiration pathways. It acts as an important metabolic checkpoint, with citrate levels modulating further steps in the Krebs cycle and providing feedback inhibition for glycolysis. Citrate synthase’s activity influences overall metabolic rate, and its dysfunction has been linked to disease states such as cancer, where reduced citrate synthase activity is associated with more malignant cell phenotypes, metabolic disorders, including diabetes, and mitochondrial diseases affecting energy homeostasis.
    References
    • https://pubmed.ncbi.nlm.nih.gov/37393492/
    • https://pubmed.ncbi.nlm.nih.gov/35873436/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
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