Zidovudine

Catalog No.S2579 Synonyms: Azidothymidine, NSC 602670

Zidovudine  Chemical Structure

Molecular Weight(MW): 267.24

Zidovudine (ZDV) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.

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Biological Activity

Description Zidovudine (ZDV) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.
Targets
Reverse transcriptase [1]
In vitro

Zidovudine pretreatment has potent anti-HIV-1 activity in the newly infected T and monocytic cells but not in chronically infected cells. [1] Inhibition of reverse transcription by Zidovudine decreases p24 antigen levels modestly, decreased HIV-1 gag by 19-fold, and inhibits detection of 2-LTR HIV-1 DNA. [2] Zidovudine and dideoxynucleosides deplete wild-type mitochondrial DNA levels and increase deletedmitochondrial DNA levels in cultured Kearns-Sayre syndrome fibroblasts. [3] Zidovudine (AZT, 0.1-50 mM) has a concentration dependent suppressive effect on the growth of granulocyte-monocyte colony forming unit (CFU-GM) derived colonies. Zidovudine exposure also induces a concentration dependent suppressive effect (35-90%) on GM-CSF receptor type alpha (GM-CSFR alpha) gene expression. Zidovudine causes a much lower decrease (15-22%) on the IL-3 receptor type alpha (IL-3R alpha) message level, and has an insignificant effect on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and c-myc message levels. [4] Zidovudine causes a concentration-dependent inhibition in the levels of the mRNA of Epo receptors and c-fos, whereas the level of c-myc mRNA is unaffected. Zidovudine also inhibits protein kinase C (PKC) activity in a concentration- and time-dependent manner, causing 50% inhibition at 10 mM within 3 hours. Zidovudine-induced down-regulation of Epo receptors and c-fos expression coupled with inhibition of Epo receptor-mediated signal transduction through PKC are significant contributory factors to AZT-induced erythroid toxicity. [5] Zidovudine could decrease the HDR efficiency. It decrease CRISPR-mediated sequence-specific genome knockin events while increases knockout efficiency[6] .

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human C8166 cells MofDSpVv[3Srb36gZZN{[Xl? MWjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhcW6oZXP0[YQhcW5iaIXtZY4hSzhzNk[gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0MEBGSzVyPUTlMVA3KM7:TR?= NH63b4czOTV|NEW0NC=>
PBMC NXLmOYhxTnWwY4Tpc44h[XO|YYm= NGrh[GoyKM7:TR?= MXLBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKFSHS1mgdoVxdGmlYYTpc44hcW5iUFLNR{BifCBzIIXNMEBKSzVyPUCuNFAxOTRizszN M4PXZVE3PDJyMEK3
human H9 cells NXj1[Gt{TnWwY4Tpc44h[XO|YYm= NUDL[HlRPiCmYYnz MVzBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhO0JiaX7m[YN1\WRiaX6gbJVu[W5iSEmgZ4VtdHNiaX7m[YN1\WRid3n0bEA3NjJ3IIXMJI9nKH[rcoXzJJN1d2OtIHHzd4V{e2WmIHHzJIV5eHKnc4Ppc44hd2ZicEK0JIFvfGmpZX6gZYZ1\XJiNjDkZZl{KHCxc4TpcoZm[3Srb36gZpkhTUyLU1GsJGlEPTB;MD6zJI5O NEfBSGUzODB6NkG0PS=>
AA5 cells NFv4e3pHfW6ldHnvckBie3OjeR?= M1fSUGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JI9nKEi3bXHuJIludXWwb3Tl[olkcWWwY4mgeolzfXNiMTCzRkBqdm[nY4Tl[EBqdiCDQUWgZ4VtdHNiaX7m[YN1\WRid3n0bEAyNjV4IIXMJI9nKH[rcoXzJJN1d2OtIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhWmW4ZYLz[UB1emGwc3PybZB1[XOnIHL5JHs{UF2WVGCgbY5kd3Kyb4LheIlwdiCjc4PhfUwhUUN3ME2wMlMhdk1? NEfoOIUzODB6NkG0PS=>
CEM cells MYTGeY5kfGmxbjDhd5NigQ>? MXPDc45k\W62cnH0bY9vKG:oIITo[UBlenWpIILld5VtfGmwZzDpckA2OCVicnXkeYN1cW:wIH;mJJRp\SC4aYLhcEBkgXSxcHH0bIlkKGWoZnXjeEBi\2GrboP0JGhKXi1zIILldIxq[2G2aX;uJIlvKEOHTTDj[YxteyxiRVO1NF0xNjN{IH7N NIfFSYYzOTV|MkC2
MT4 cells NGjVXnFHfW6ldHnvckBie3OjeR?= NXXCbHhkPCCmYYnz MnfLRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdpV{NWmwZIXj[YQh[3m2b4DheIhw\2WwaXPpeJkhcW5iTWS0JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[XluIFXDOVA:OC55IH7N NI\ISGwyPzl4NEe5Oi=>
human MT2 cells M1XRPWZ2dmO2aX;uJIF{e2G7 Mny4NUBp MVrBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDITXYyKHO3YoT5dIUhSi1|QjDpcoZm[3SnZDDpckAyKGi{LYDy[ZRz\WG2ZXSgbJVu[W5iTWSyJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZibYXseIlkgWOuZTDy[ZBtcWOjdHnvckBu\WG|dYLl[EBwdiCmYYmgOUBxd3O2aX7m[YN1cW:wIHL5JHJVKFOSQTygSWM2OD1zLkKgcm0> NEflSXQyQDNzNkWyNS=>
C8166 cells NEfWWo5HfW6ldHnvckBie3OjeR?= M{\obGlvcGmkaYTpc44hd2ZiSFnWNU0{SiC{ZYDsbYNifGmxbjDpckBEQDF4NjDj[YxteyxiRVO1NF0yNjlibl2= MY[xOlI4QTd5Mx?=
CEM-SS cell M2PFOWZ2dmO2aX;uJIF{e2G7 NH\FOnFKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhfGinIFjJWk0yNWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5QhcW5iQ1XNMXNUKGOnbHygcIlv\SxiSVO1NF0xNjByMjFOwG0> NFf0Wo84PjVyNke4
PBLs MmXISpVv[3Srb36gZZN{[Xl? MUPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDzeYJ1gXCnIHnzc4xifGViRzDzeJJicW5iaX6gVGJNeyBqcHXybZBp\XKjbDDicI9w\CCueX3wbI9kgXSnczmsJGlEPTB;MD6wNFIh|ryP M1rZPFEyPzB6OUGz
human lymphocyte CEM/0 cell line M33tNmZ2dmO2aX;uJIF{e2G7 NXezdmg2SW62aTDITXYuOSCjY4Tpeol1gSCrbjDoeY1idiCueX3wbI9kgXSnIFPFUU8xKGOnbHygcIlv\SxiRVO1NF0xNjByMzFOwG0> NGjrd3o5PDd6OUC0
Jurkat cell Mnz5SpVv[3Srb36gZZN{[Xl? Ml7qTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiSFnWMVEhcW6oZXP0[YQhUnW{a3H0JINmdGxibHnu[ZMtKEmFNUC9NE4xOSEQvF2= NXfTVGdPPzh|N{KyNC=>
human H9 cells NXzSOXN2S3m2b4TvfIlkcXS7IHHzd4F6 NXrscoo5PiCmYYnz MnXLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFkh[2WubIOgZYZ1\XJiNjDkZZl{KGK7IF3UWEBie3OjeTygSWM2OD1yLkCxJO69VQ>? MV[yNFg1Pjh4OB?=
C3H/3T3 cells MWXGeY5kfGmxbjDhd5NigQ>? NIfSWpZEd26lZX70doF1cW:wIH;mJINwdXCxdX7kJJJmeXWrcnXkJJRwKGmwaHnibZQhUEmYLUGgbY5lfWOnZDDjfZRweGG2aH;n[Y5q[2m2eTDv[kBOW1ZvaX7keYNm\CC2cnHud4Zwem2jdHnvckBw\iCFM1ivN3Q{KGOnbHzzJIJ6KDVyJTygSWM2OD1yLkCyJO69VQ>? MVSyNFE3PzF6
U937 cells Ml3TSpVv[3Srb36gZZN{[Xl? MU\F[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CCob4KgZY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgUYFkem:yaHHn[UBk\WyuIHzpcoUhd2ZiVUmzO{Bk\WyuczDv[kBJfW2jbjDifUBZXFRiYYPzZZktKEWFNUC9NE4xOyEQvF2= MlzzO|k{OjV{Nh?=

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 53 mg/mL (198.32 mM)
Water 53 mg/mL warmed (198.32 mM)
Ethanol 18 mg/mL warmed (67.35 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.24
Formula

C10H13N5O4

CAS No. 30516-87-1
Storage powder
in solvent
Synonyms Azidothymidine, NSC 602670

Bio Calculators

Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00528957 Completed HIV Infections Gilead Sciences December 28 2006 Phase 3
NCT01714414 Completed HIV-1 Infection Michael Hoelscher|European and Developing Countries Clinical Trials Partnership (EDCTP)|German Federal Ministry of Education and Research|National Institute for Medical Research Tanzania|Treichville Academic hospital center Division of infectious and tropical diseases (SMIT)|French National Institute for Health and Medical Research-French National Agency for Research on AIDS and Viral Hepatitis (Inserm-ANRS)|Kumasi Centre for Collaborative Research (KCCR)|Bernhard Nocht Institute for Tropical Medicine|Pharmaceutical Company (Chiracon GmbH)|Pharmaceutical Company (STADA Vietnam Joint Venture Co. Ltd.)|Ludwig-Maximilians - University of Munich December 2012 Phase 2
NCT01540240 Unknown status HIV|Zidovudine Adverse Reaction University Hospital Geneva August 2011 Phase 2|Phase 3
NCT00672412 Completed HIV Infections National Institute of Allergy and Infectious Diseases (NIAID)|International Maternal Pediatric Adolescent AIDS Clinical Trials Group|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) October 2008 Phase 1|Phase 2
NCT00854581 Terminated Lymphoma|Precancerous/Nonmalignant Condition University of Miami|National Cancer Institute (NCI) November 2007 Phase 4
NCT00470041 Completed Healthy University Ghent June 2007 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    Do you happen to have any information regarding the half-life of AZT?

  • Answer:

    The Half-life of S2579 in human is available (http://www.rxlist.com/retrovir-drug/clinical-pharmacology.htm), about 0.5-3 hours in adult subjects.

Reverse Transcriptase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID