Catalog No.S2579 Synonyms: Azidothymidine, NSC 602670

For research use only.

Zidovudine (ZDV, Azidothymidine, NSC 602670) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.

Zidovudine  Chemical Structure

CAS No. 30516-87-1

Selleck's Zidovudine has been cited by 14 publications

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Biological Activity

Description Zidovudine (ZDV, Azidothymidine, NSC 602670) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.
Reverse transcriptase [1]
In vitro

Zidovudine pretreatment has potent anti-HIV-1 activity in the newly infected T and monocytic cells but not in chronically infected cells. [1] Inhibition of reverse transcription by Zidovudine decreases p24 antigen levels modestly, decreased HIV-1 gag by 19-fold, and inhibits detection of 2-LTR HIV-1 DNA. [2] Zidovudine and dideoxynucleosides deplete wild-type mitochondrial DNA levels and increase deletedmitochondrial DNA levels in cultured Kearns-Sayre syndrome fibroblasts. [3] Zidovudine (AZT, 0.1-50 mM) has a concentration dependent suppressive effect on the growth of granulocyte-monocyte colony forming unit (CFU-GM) derived colonies. Zidovudine exposure also induces a concentration dependent suppressive effect (35-90%) on GM-CSF receptor type alpha (GM-CSFR alpha) gene expression. Zidovudine causes a much lower decrease (15-22%) on the IL-3 receptor type alpha (IL-3R alpha) message level, and has an insignificant effect on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and c-myc message levels. [4] Zidovudine causes a concentration-dependent inhibition in the levels of the mRNA of Epo receptors and c-fos, whereas the level of c-myc mRNA is unaffected. Zidovudine also inhibits protein kinase C (PKC) activity in a concentration- and time-dependent manner, causing 50% inhibition at 10 mM within 3 hours. Zidovudine-induced down-regulation of Epo receptors and c-fos expression coupled with inhibition of Epo receptor-mediated signal transduction through PKC are significant contributory factors to AZT-induced erythroid toxicity. [5] Zidovudine could decrease the HDR efficiency. It decrease CRISPR-mediated sequence-specific genome knockin events while increases knockout efficiency[6] .

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human C8166 cells NYLHSnBpTnWwY4Tpc44h[XO|YYm= MmDERY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iaX3teY5w\GWoaXPp[Y5kgSC4aYL1d{AyKGmwZnXjeIVlKGmwIHj1cYFvKEN6MU[2JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyeZMucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEwhTUN3ME20[U0xPiEQvF2= NHf3R2ozOTV|NEW0NC=>
human H9 cells M33XR2Z2dmO2aX;uJIF{e2G7 MnvKOkBl[Xm| NHjVcVFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gTFkh[2WubIOgbY5n\WO2ZXSge4l1cCB4LkK1JJVNKG:oII\pdpV{KHO2b3PrJIF{e2W|c3XkJIF{KGW6cILld5Nqd25ib3[gdFI1KGGwdHnn[Y4h[W[2ZYKgOkBl[Xm|IIDvd5Rqdm[nY4Tpc44h[nliRVzJV2EtKEmFNUC9NE4{KG6P MUCyNFA5PjF2OR?=
AA5 cells NYrDXYFrTnWwY4Tpc44h[XO|YYm= MXzBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDv[kBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiM1KgbY5n\WO2ZXSgbY4hSUF3IHPlcIx{KGmwZnXjeIVlKHerdHigNU42PiC3TDDv[kB3cXK3czDzeI9kcyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJHJmfmW{c3WgeJJidnOlcnnweIF{\SCkeTDbN2heXFSSIHnuZ49zeG:{YYTpc44h[XO|YYmsJGlEPTB;MD6zJI5O M37aSVIxODh4MUS5
CEM cells M2HFWGZ2dmO2aX;uJIF{e2G7 MY\Dc45k\W62cnH0bY9vKG:oIITo[UBlenWpIILld5VtfGmwZzDpckA2OCVicnXkeYN1cW:wIH;mJJRp\SC4aYLhcEBkgXSxcHH0bIlkKGWoZnXjeEBi\2GrboP0JGhKXi1zIILldIxq[2G2aX;uJIlvKEOHTTDj[YxteyxiRVO1NF0xNjN{IH7N NVfESYVLOjF3M{KwOi=>
MT4 cells NXPRTppDTnWwY4Tpc44h[XO|YYm= M2LJR|Qh\GG7cx?= NGHwPIVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolzfXNvaX7keYNm\CCleYTvdIF1cG:pZX7pZ4l1gSCrbjDNWFQh[2WubIOgZYZ1\XJiNDDkZZl{KGK7IF3UWEBie3OjeTygSWM2OD1yLkegcm0> NXLCOotzOTd7NkS3PVY>
human MT2 cells M3TuZ2Z2dmO2aX;uJIF{e2G7 MnfWNUBp MnH5RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSC|dXL0fZBmKEJvM1KgbY5n\WO2ZXSgbY4hOSCqcj3wdoV1emWjdHXkJIh2dWGwIF3UNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIH31cJRq[3mlbHWgdoVxdGmlYYTpc44hdWWjc4Xy[YQhd25iZHH5JFUheG:|dHnu[oVkfGmxbjDifUBTXCCVUFGsJGVEPTB;MT6yJI5O NEfwTm0yQDNzNkWyNS=>
C8166 cells M2Lqb2Z2dmO2aX;uJIF{e2G7 M{f6[WlvcGmkaYTpc44hd2ZiSFnWNU0{SiC{ZYDsbYNifGmxbjDpckBEQDF4NjDj[YxteyxiRVO1NF0yNjlibl2= NHnGcnEyPjJ5OUe3Ny=>
PBLs NVr0[HVoTnWwY4Tpc44h[XO|YYm= M2TTOGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JJN2[nS7cHWgbZNwdGG2ZTDHJJN1emGrbjDpckBRSky|IDjw[ZJqeGincnHsJIJtd2:mIHz5cZBpd2O7dHXzLUwhUUN3ME2wMlAxOiEQvF2= MmHONVE4ODh7MUO=
human lymphocyte CEM/0 cell line NXXqWmRnTnWwY4Tpc44h[XO|YYm= M13MWGFvfGliSFnWMVEh[WO2aY\peJkhcW5iaIXtZY4hdHmvcHjvZ5l1\SCFRV2vNEBk\WyuIHzpcoUtKEWFNUC9NE4xODNizszN Mn:xPFQ4QDlyNB?=
Jurkat cell MoD0SpVv[3Srb36gZZN{[Xl? MoPBTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiSFnWMVEhcW6oZXP0[YQhUnW{a3H0JINmdGxibHnu[ZMtKEmFNUC9NE4xOSEQvF2= NVLkTXlpPzh|N{KyNC=>
human H9 cells M4Xv[WN6fG:2b4jpZ4l1gSCjc4PhfS=> MoDyOkBl[Xm| MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIPUBk\WyuczDh[pRmeiB4IHThfZMh[nliTWTUJIF{e2G7LDDFR|UxRTBwMEGg{txO MXeyNFg1Pjh4OB?=
C3H/3T3 cells NXPwSHZ3TnWwY4Tpc44h[XO|YYm= NWrzeJRZS2:wY3XueJJifGmxbjDv[kBkd22yb4Xu[EBz\XG3aYLl[EB1dyCrbnjpZol1KEiLVj2xJIlv\HWlZXSgZ5l1d3CjdHjv[4VvcWOrdImgc4YhVVOYLXnu[JVk\WRidILhcpNnd3KvYYTpc44hd2ZiQ{PIM|NVOyClZXzsd{BjgSB3MDWsJGVEPTB;MD6wNkDPxE1? MVWyNFE3PzF6
U937 cells NIP2UppHfW6ldHnvckBie3OjeR?= M3XydGVn\mWldHn2[UBkd26lZX70doF1cW:wIILldZVqemWmIH\vdkBidnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBO[WO{b4DoZYdmKGOnbHygcIlv\SCxZjDVPVM4KGOnbHzzJI9nKEi3bXHuJIJ6KFiWVDDhd5NigSxiRVO1NF0xNjB|IN88US=> Moi0O|k{OjV{Nh?=

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 53 mg/mL
(198.32 mM)
Water 53 mg/mL warmed
(198.32 mM)
Ethanol 18 mg/mL warmed
(67.35 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.24


CAS No. 30516-87-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)N=[N+]=[N-]

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01818258 Completed Drug: ZDV+3TC+LPV/r HIV Positive|Malnourished International Maternal Pediatric Adolescent AIDS Clinical Trials Group|National Institute of Allergy and Infectious Diseases (NIAID)|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|National Institute of Mental Health (NIMH) October 26 2015 Phase 4

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

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Frequently Asked Questions

Question 1:
Do you happen to have any information regarding the half-life of AZT?

The Half-life of S2579 in human is available (http://www.rxlist.com/retrovir-drug/clinical-pharmacology.htm), about 0.5-3 hours in adult subjects.

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