Zidovudine

Catalog No.S2579 Synonyms: Azidothymidine, NSC 602670

Zidovudine  Chemical Structure

Molecular Weight(MW): 267.24

Zidovudine (ZDV) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.

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Biological Activity

Description Zidovudine (ZDV) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.
Targets
Reverse transcriptase [1]
In vitro

Zidovudine pretreatment has potent anti-HIV-1 activity in the newly infected T and monocytic cells but not in chronically infected cells. [1] Inhibition of reverse transcription by Zidovudine decreases p24 antigen levels modestly, decreased HIV-1 gag by 19-fold, and inhibits detection of 2-LTR HIV-1 DNA. [2] Zidovudine and dideoxynucleosides deplete wild-type mitochondrial DNA levels and increase deletedmitochondrial DNA levels in cultured Kearns-Sayre syndrome fibroblasts. [3] Zidovudine (AZT, 0.1-50 mM) has a concentration dependent suppressive effect on the growth of granulocyte-monocyte colony forming unit (CFU-GM) derived colonies. Zidovudine exposure also induces a concentration dependent suppressive effect (35-90%) on GM-CSF receptor type alpha (GM-CSFR alpha) gene expression. Zidovudine causes a much lower decrease (15-22%) on the IL-3 receptor type alpha (IL-3R alpha) message level, and has an insignificant effect on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and c-myc message levels. [4] Zidovudine causes a concentration-dependent inhibition in the levels of the mRNA of Epo receptors and c-fos, whereas the level of c-myc mRNA is unaffected. Zidovudine also inhibits protein kinase C (PKC) activity in a concentration- and time-dependent manner, causing 50% inhibition at 10 mM within 3 hours. Zidovudine-induced down-regulation of Epo receptors and c-fos expression coupled with inhibition of Epo receptor-mediated signal transduction through PKC are significant contributory factors to AZT-induced erythroid toxicity. [5] Zidovudine could decrease the HDR efficiency. It decrease CRISPR-mediated sequence-specific genome knockin events while increases knockout efficiency[6] .

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human C8166 cells MVjGeY5kfGmxbjDhd5NigQ>? NHHQbm1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiaX7m[YN1\WRiaX6gbJVu[W5iQ{ixOlYh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1NCCHQ{WwQVRmNTB4IN88US=> NHrzbIszOTV|NEW0NC=>
PBMC NFLvZppHfW6ldHnvckBie3OjeR?= NHTLW|gyKM7:TR?= NIHZZo5CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIGTFT2khemWybHnjZZRqd25iaX6gVGJOSyCjdDCxJJVONCCLQ{WwQVAvODByMUSg{txO M1THdFE3PDJyMEK3
human H9 cells NI[xTW1HfW6ldHnvckBie3OjeR?= M1v1NFYh\GG7cx?= MYnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhO0JiaX7m[YN1\WRiaX6gbJVu[W5iSEmgZ4VtdHNiaX7m[YN1\WRid3n0bEA3NjJ3IIXMJI9nKH[rcoXzJJN1d2OtIHHzd4V{e2WmIHHzJIV5eHKnc4Ppc44hd2ZicEK0JIFvfGmpZX6gZYZ1\XJiNjDkZZl{KHCxc4TpcoZm[3Srb36gZpkhTUyLU1GsJGlEPTB;MD6zJI5O MnvzNlAxQDZzNEm=
AA5 cells NIXQSYZHfW6ldHnvckBie3OjeR?= M1:2XmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JI9nKEi3bXHuJIludXWwb3Tl[olkcWWwY4mgeolzfXNiMTCzRkBqdm[nY4Tl[EBqdiCDQUWgZ4VtdHNiaX7m[YN1\WRid3n0bEAyNjV4IIXMJI9nKH[rcoXzJJN1d2OtIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhWmW4ZYLz[UB1emGwc3PybZB1[XOnIHL5JHs{UF2WVGCgbY5kd3Kyb4LheIlwdiCjc4PhfUwhUUN3ME2wMlMhdk1? MWKyNFA5PjF2OR?=
CEM cells M37zemZ2dmO2aX;uJIF{e2G7 M{f3W2NwdmOnboTyZZRqd25ib3[geIhmKGS{dXegdoV{fWy2aX7nJIlvKDVyJTDy[YR2[3Srb36gc4YhfGinII\pdoFtKGO7dH;wZZRpcWNiZX\m[YN1KGGpYXnud5QhUEmYLUGgdoVxdGmlYYTpc44hcW5iQ1XNJINmdGy|LDDFR|UxRTBwM{Kgcm0> NF3Qe2szOTV|MkC2
MT4 cells NX;JZohZTnWwY4Tpc44h[XO|YYm= MljWOEBl[Xm| MorHRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGlXOSB|QjDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdpV{NWmwZIXj[YQh[3m2b4DheIhw\2WwaXPpeJkhcW5iTWS0JINmdGy|IHHmeIVzKDRiZHH5d{BjgSCPVGSgZZN{[XluIFXDOVA:OC55IH7N Mnq2NVc6PjR5OU[=
human MT2 cells MmXSSpVv[3Srb36gZZN{[Xl? MV[xJIg> NH7LTllCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIIP1ZpR6eGViQj2zRkBqdm[nY4Tl[EBqdiBzIHjyMZBz\XS{ZXH0[YQhcHWvYX6gUXQzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gcZVtfGmleXPs[UBz\XCuaXPheIlwdiCvZXHzeZJm\CCxbjDkZZkhPSCyb4P0bY5n\WO2aX;uJIJ6KFKWIGPQRUwhTUN3ME2xMlIhdk1? M2HQO|E5OzF4NUKx
C8166 cells MXnGeY5kfGmxbjDhd5NigQ>? Ml\JTY5pcWKrdHnvckBw\iCKSW[xMVNDKHKncHzpZ4F1cW:wIHnuJGM5OTZ4IHPlcIx{NCCHQ{WwQVEvQSCwTR?= NGK2ZlMyPjJ5OUe3Ny=>
CEM-SS cell NXXHe4ttTnWwY4Tpc44h[XO|YYm= M33tfWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEB1cGViSFnWMVEucW6mdXPl[EBkgXSxcHH0bIlkKGWoZnXjeEBqdiCFRV2tV3Mh[2WubDDsbY5mNCCLQ{WwQVAvODB{IN88US=> Ml;DO|Y2ODZ5OB?=
PBLs NWfO[YsyTnWwY4Tpc44h[XO|YYm= MnvXRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4Sgd5VjfHmyZTDpd49t[XSnIFegd5Rz[WmwIHnuJHBDVHNiKIDldolxcGW{YXygZoxwd2RibIntdIhw[3m2ZYOpMEBKSzVyPUCuNFAzKM7:TR?= NUTIOJVXOTF5MEi5NVM>
human lymphocyte CEM/0 cell line M161O2Z2dmO2aX;uJIF{e2G7 NVvYfHR6SW62aTDITXYuOSCjY4Tpeol1gSCrbjDoeY1idiCueX3wbI9kgXSnIFPFUU8xKGOnbHygcIlv\SxiRVO1NF0xNjByMzFOwG0> MknXPFQ4QDlyNB?=
Jurkat cell Ml7HSpVv[3Srb36gZZN{[Xl? NIfNTnhKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDITXYuOSCrbn\lZ5Rm\CCMdYLrZZQh[2WubDDsbY5meyxiSVO1NF0xNjBzIN88US=> NYLyeVFEPzh|N{KyNC=>
human H9 cells NEP1XZBEgXSxdH;4bYNqfHliYYPzZZk> MkfWOkBl[Xm| MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIPUBk\WyuczDh[pRmeiB4IHThfZMh[nliTWTUJIF{e2G7LDDFR|UxRTBwMEGg{txO NVzqTlR[OjB6NE[4Olg>
C3H/3T3 cells NXrBSZNlTnWwY4Tpc44h[XO|YYm= MoTYR49v[2WwdILheIlwdiCxZjDjc41xd3WwZDDy[ZF2cXKnZDD0c{BqdmirYnn0JGhKXi1zIHnu[JVk\WRiY4n0c5BifGixZ3XubYNqfHlib3[gUXNXNWmwZIXj[YQhfHKjboPmc5Ju[XSrb36gc4YhSzOKL{PUN{Bk\WyuczDifUA2OCVuIFXDOVA:OC5yMjFOwG0> MXGyNFE3PzF6
U937 cells NHfuRYxHfW6ldHnvckBie3OjeR?= Ml\4SYZn\WO2aY\lJINwdmOnboTyZZRqd25icnXxeYlz\WRiZn;yJIFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JG1i[3KxcHjh[4Uh[2WubDDsbY5mKG:oIGW5N|ch[2WubIOgc4YhUHWvYX6gZpkhYFSWIHHzd4F6NCCHQ{WwQVAvODNizszN NULRN41KPzl|MkWyOi=>

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 53 mg/mL (198.32 mM)
Water 53 mg/mL warmed (198.32 mM)
Ethanol 18 mg/mL warmed (67.35 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 267.24
Formula

C10H13N5O4

CAS No. 30516-87-1
Storage powder
in solvent
Synonyms Azidothymidine, NSC 602670

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02229760 Terminated Drug: Tipranavir capsules|Drug: Ritonavir capsules HIV Infections Boehringer Ingelheim August 2006 Phase 1|Phase 2
NCT01386970 Completed Drug: zidovudine 300mg and lamivudine 150mg as Combivir HIV University of Colorado Denver|National Institute of Allergy and Infectious Diseases (NIAID)|University of Hawaii May 2005 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    Do you happen to have any information regarding the half-life of AZT?

  • Answer:

    The Half-life of S2579 in human is available (http://www.rxlist.com/retrovir-drug/clinical-pharmacology.htm), about 0.5-3 hours in adult subjects.

Reverse Transcriptase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID