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NU7441 (KU-57788)

Name: NU7441 (KU-57788)
Brand: Selleck Chemicals
SKU: S2638
Rating: 5 (296 reviews)

Catalog No.S2638 Purity:99.94%

NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.

NU7441 (KU-57788) Chemical Structure

CAS No. 503468-95-9

Purity & Quality Control

Batch: Purity: 99.94%

99.94

Products often used together with NU7441 (KU-57788)

KU-0060648

NU7441 and KU-0060648 reduce the frequency of the non-homologous end joining (NHEJ) pathway while increasing the rate of homology-directed recombination (HDR).

Robert F, et al. Genome Med. 2015 Aug 27;7(1):93.

KU-55933

NU7441 and KU-55933 combination treatment significantly increase X-ray cytotoxicity in MDCK cells.

Koike M, et al. J Vet Med Sci. 2022 Nov; 84(11): 1485–1490.

NU7026

NU7441 and NU7026 are potent DNA-PK inhibitors that inhibit the DSBs repair pathway and enhance the sensitivity of cancer cells to ionizing radiation or chemo-potentiation.

Li LY, et al. Front Pharmacol. 2021 Feb 8;11:629266.

NU7441 (KU-57788) Related Products

Related Products	NU7026 AZD7648 CC-115 Nedisertib (M3814) KU-0060648 YU238259 LTURM34	Click to Expand
Related Compound Libraries	FDA-approved Drug Library Natural Product Library Apoptosis Compound Library DNA Damage/DNA Repair compound Library Cell Cycle compound library	Click to Expand

Signaling Pathway

DNA-PK Signaling Pathway

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
NCI-H1770	Growth Inhibition Assay			IC50=0.02143 μM	SANGER
A172	Growth Inhibition Assay			IC50=0.15316 μM	SANGER
NCI-H526	Growth Inhibition Assay			IC50=0.28407 μM	SANGER
HDLM-2	Growth Inhibition Assay			IC50=0.32345 μM	SANGER
HuO-3N1	Growth Inhibition Assay			IC50=0.46636 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay			IC50=0.60994 μM	SANGER
SK-N-AS	Growth Inhibition Assay			IC50=0.62216 μM	SANGER
LoVo	Growth Inhibition Assay			IC50=0.86749 μM	SANGER
LAMA-84	Growth Inhibition Assay			IC50=0.98833 μM	SANGER
CTB-1	Growth Inhibition Assay			IC50=1.11546 μM	SANGER
KP-N-YS	Growth Inhibition Assay			IC50=1.14231 μM	SANGER
HH	Growth Inhibition Assay			IC50=1.18093 μM	SANGER
CHL-1	Growth Inhibition Assay			IC50=1.21368 μM	SANGER
NB7	Growth Inhibition Assay			IC50=1.24745 μM	SANGER
HL-60	Growth Inhibition Assay			IC50=1.3819 μM	SANGER
639-V	Growth Inhibition Assay			IC50=1.44128 μM	SANGER
NCI-H1793	Growth Inhibition Assay			IC50=1.4584 μM	SANGER
NEC8	Growth Inhibition Assay			IC50=1.5535 μM	SANGER
PA-1	Growth Inhibition Assay			IC50=1.58294 μM	SANGER
D-423MG	Growth Inhibition Assay			IC50=1.69361 μM	SANGER
NCI-H292	Growth Inhibition Assay			IC50=1.74013 μM	SANGER
SK-MEL-1	Growth Inhibition Assay			IC50=1.79479 μM	SANGER
LB2241-RCC	Growth Inhibition Assay			IC50=2.14146 μM	SANGER
SK-MEL-3	Growth Inhibition Assay			IC50=2.1649 μM	SANGER
MHH-PREB-1	Growth Inhibition Assay			IC50=2.16609 μM	SANGER
BFTC-905	Growth Inhibition Assay			IC50=2.16687 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay			IC50=2.19909 μM	SANGER
G-361	Growth Inhibition Assay			IC50=2.2168 μM	SANGER
MOLT-4	Growth Inhibition Assay			IC50=2.21888 μM	SANGER
DEL	Growth Inhibition Assay			IC50=2.35564 μM	SANGER
MV-4-11	Growth Inhibition Assay			IC50=2.3739 μM	SANGER
SK-NEP-1	Growth Inhibition Assay			IC50=2.38783 μM	SANGER
RS4-11	Growth Inhibition Assay			IC50=2.56023 μM	SANGER
HuH-7	Growth Inhibition Assay			IC50=2.56702 μM	SANGER
A101D	Growth Inhibition Assay			IC50=2.61338 μM	SANGER
KG-1	Growth Inhibition Assay			IC50=2.67924 μM	SANGER
KALS-1	Growth Inhibition Assay			IC50=2.69633 μM	SANGER
VMRC-RCZ	Growth Inhibition Assay			IC50=2.77328 μM	SANGER
SK-UT-1	Growth Inhibition Assay			IC50=3.1531 μM	SANGER
SW962	Growth Inhibition Assay			IC50=3.20352 μM	SANGER
DBTRG-05MG	Growth Inhibition Assay			IC50=3.28085 μM	SANGER
PANC-08-13	Growth Inhibition Assay			IC50=3.41278 μM	SANGER
QIMR-WIL	Growth Inhibition Assay			IC50=3.41341 μM	SANGER
SK-MEL-30	Growth Inhibition Assay			IC50=3.42598 μM	SANGER
697	Growth Inhibition Assay			IC50=3.44993 μM	SANGER
NY	Growth Inhibition Assay			IC50=3.45022 μM	SANGER
ES7	Growth Inhibition Assay			IC50=3.46729 μM	SANGER
JVM-3	Growth Inhibition Assay			IC50=3.48077 μM	SANGER
U031	Growth Inhibition Assay			IC50=3.59368 μM	SANGER
BHT-101	Growth Inhibition Assay			IC50=3.59985 μM	SANGER
8305C	Growth Inhibition Assay			IC50=3.61736 μM	SANGER
MG-63	Growth Inhibition Assay			IC50=3.62676 μM	SANGER
NOS-1	Growth Inhibition Assay			IC50=3.65312 μM	SANGER
YH-13	Growth Inhibition Assay			IC50=3.87189 μM	SANGER
LXF-289	Growth Inhibition Assay			IC50=3.91041 μM	SANGER
GOTO	Growth Inhibition Assay			IC50=3.9149 μM	SANGER
EKVX	Growth Inhibition Assay			IC50=4.22524 μM	SANGER
CAL-51	Growth Inhibition Assay			IC50=4.24854 μM	SANGER
AN3-CA	Growth Inhibition Assay			IC50=4.25813 μM	SANGER
IA-LM	Growth Inhibition Assay			IC50=4.27935 μM	SANGER
KU-19-19	Growth Inhibition Assay			IC50=4.32921 μM	SANGER
A704	Growth Inhibition Assay			IC50=4.40888 μM	SANGER
RH-18	Growth Inhibition Assay			IC50=4.44953 μM	SANGER
IST-MEL1	Growth Inhibition Assay			IC50=4.58263 μM	SANGER
LU-134-A	Growth Inhibition Assay			IC50=4.59685 μM	SANGER
SF126	Growth Inhibition Assay			IC50=4.60715 μM	SANGER
EFO-27	Growth Inhibition Assay			IC50=4.66525 μM	SANGER
SN12C	Growth Inhibition Assay			IC50=4.71898 μM	SANGER
L-363	Growth Inhibition Assay			IC50=4.77116 μM	SANGER
MEL-HO	Growth Inhibition Assay			IC50=4.77989 μM	SANGER
TYK-nu	Growth Inhibition Assay			IC50=4.80128 μM	SANGER
MDA-MB-415	Growth Inhibition Assay			IC50=5.00314 μM	SANGER
HCC70	Growth Inhibition Assay			IC50=5.03939 μM	SANGER
IGROV-1	Growth Inhibition Assay			IC50=5.25292 μM	SANGER
NCI-H1355	Growth Inhibition Assay			IC50=5.296 μM	SANGER
23132-87	Growth Inhibition Assay			IC50=5.32024 μM	SANGER
NB69	Growth Inhibition Assay			IC50=5.3242 μM	SANGER
HSC-2	Growth Inhibition Assay			IC50=5.41773 μM	SANGER
KYSE-410	Growth Inhibition Assay			IC50=5.47636 μM	SANGER
GCIY	Growth Inhibition Assay			IC50=5.51378 μM	SANGER
A427	Growth Inhibition Assay			IC50=5.53661 μM	SANGER
ChaGo-K-1	Growth Inhibition Assay			IC50=5.56599 μM	SANGER
PFSK-1	Growth Inhibition Assay			IC50=5.62194 μM	SANGER
MLMA	Growth Inhibition Assay			IC50=5.83606 μM	SANGER
RVH-421	Growth Inhibition Assay			IC50=6.07338 μM	SANGER
LB1047-RCC	Growth Inhibition Assay			IC50=6.17522 μM	SANGER
MHH-NB-11	Growth Inhibition Assay			IC50=6.32721 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay			IC50=6.63291 μM	SANGER
UM-UC-3	Growth Inhibition Assay			IC50=6.64334 μM	SANGER
IST-SL1	Growth Inhibition Assay			IC50=6.69369 μM	SANGER
NB14	Growth Inhibition Assay			IC50=6.79761 μM	SANGER
NBsusSR	Growth Inhibition Assay			IC50=6.82502 μM	SANGER
D-263MG	Growth Inhibition Assay			IC50=6.91068 μM	SANGER
NCI-H2347	Growth Inhibition Assay			IC50=6.93213 μM	SANGER
ES8	Growth Inhibition Assay			IC50=6.97588 μM	SANGER
786-0	Growth Inhibition Assay			IC50=7.09786 μM	SANGER
EPLC-272H	Growth Inhibition Assay			IC50=7.16246 μM	SANGER
CAPAN-1	Growth Inhibition Assay			IC50=7.24359 μM	SANGER
NCI-H82	Growth Inhibition Assay			IC50=7.26659 μM	SANGER
G-402	Growth Inhibition Assay			IC50=7.30051 μM	SANGER
SW982	Growth Inhibition Assay			IC50=7.30203 μM	SANGER
OVCAR-4	Growth Inhibition Assay			IC50=7.31328 μM	SANGER
KARPAS-299	Growth Inhibition Assay			IC50=7.32622 μM	SANGER
MIA-PaCa-2	Growth Inhibition Assay			IC50=7.36823 μM	SANGER
AU565	Growth Inhibition Assay			IC50=7.59833 μM	SANGER
GAMG	Growth Inhibition Assay			IC50=7.64131 μM	SANGER
HuO9	Growth Inhibition Assay			IC50=7.71743 μM	SANGER
ACN	Growth Inhibition Assay			IC50=7.7433 μM	SANGER
RO82-W-1	Growth Inhibition Assay			IC50=7.74533 μM	SANGER
NKM-1	Growth Inhibition Assay			IC50=7.74955 μM	SANGER
CAL-12T	Growth Inhibition Assay			IC50=7.94352 μM	SANGER
FADU	Growth Inhibition Assay			IC50=8.03654 μM	SANGER
NCI-H28	Growth Inhibition Assay			IC50=8.16962 μM	SANGER
LCLC-97TM1	Growth Inhibition Assay			IC50=8.21122 μM	SANGER
CHP-134	Growth Inhibition Assay			IC50=8.24185 μM	SANGER
HT-1080	Growth Inhibition Assay			IC50=8.25093 μM	SANGER
PC-3	Growth Inhibition Assay			IC50=8.27392 μM	SANGER
SNU-449	Growth Inhibition Assay			IC50=8.2793 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay			IC50=8.34686 μM	SANGER
HT	Growth Inhibition Assay			IC50=8.3649 μM	SANGER
HOS	Growth Inhibition Assay			IC50=8.37589 μM	SANGER
KNS-62	Growth Inhibition Assay			IC50=8.37762 μM	SANGER
OAW-42	Growth Inhibition Assay			IC50=8.95964 μM	SANGER
A549	Growth Inhibition Assay			IC50=9.09612 μM	SANGER
CAKI-1	Growth Inhibition Assay			IC50=9.1661 μM	SANGER
HCC1937	Growth Inhibition Assay			IC50=9.69865 μM	SANGER
GI-ME-N	Growth Inhibition Assay			IC50=9.70137 μM	SANGER
DU-4475	Growth Inhibition Assay			IC50=9.96939 μM	SANGER
EM-2	Growth Inhibition Assay			IC50=10.2426 μM	SANGER
NCI-H1755	Growth Inhibition Assay			IC50=10.2428 μM	SANGER
NCI-H1437	Growth Inhibition Assay			IC50=10.5725 μM	SANGER
VM-CUB-1	Growth Inhibition Assay			IC50=10.6164 μM	SANGER
A2058	Growth Inhibition Assay			IC50=10.6404 μM	SANGER
BPH-1	Growth Inhibition Assay			IC50=10.7145 μM	SANGER
NB5	Growth Inhibition Assay			IC50=10.7559 μM	SANGER
G-401	Growth Inhibition Assay			IC50=10.7626 μM	SANGER
CAL-72	Growth Inhibition Assay			IC50=10.8094 μM	SANGER
SK-MEL-28	Growth Inhibition Assay			IC50=10.8202 μM	SANGER
C32	Growth Inhibition Assay			IC50=10.8347 μM	SANGER
NCI-H1155	Growth Inhibition Assay			IC50=10.8376 μM	SANGER
BB49-HNC	Growth Inhibition Assay			IC50=10.8586 μM	SANGER
GMS-10	Growth Inhibition Assay			IC50=10.8971 μM	SANGER
K5	Growth Inhibition Assay			IC50=10.9071 μM	SANGER
OS-RC-2	Growth Inhibition Assay			IC50=11.0559 μM	SANGER
HTC-C3	Growth Inhibition Assay			IC50=11.0714 μM	SANGER
LB996-RCC	Growth Inhibition Assay			IC50=11.0723 μM	SANGER
NCI-H1563	Growth Inhibition Assay			IC50=11.1011 μM	SANGER
CAL-54	Growth Inhibition Assay			IC50=11.4811 μM	SANGER
WM-115	Growth Inhibition Assay			IC50=11.6824 μM	SANGER
COLO-684	Growth Inhibition Assay			IC50=11.7267 μM	SANGER
HOP-62	Growth Inhibition Assay			IC50=11.7508 μM	SANGER
NCI-H650	Growth Inhibition Assay			IC50=11.8961 μM	SANGER
TE-11	Growth Inhibition Assay			IC50=11.9315 μM	SANGER
COLO-679	Growth Inhibition Assay			IC50=11.946 μM	SANGER
FTC-133	Growth Inhibition Assay			IC50=12.0076 μM	SANGER
D-283MED	Growth Inhibition Assay			IC50=12.0569 μM	SANGER
SK-PN-DW	Growth Inhibition Assay			IC50=12.1902 μM	SANGER
BALL-1	Growth Inhibition Assay			IC50=12.2662 μM	SANGER
GT3TKB	Growth Inhibition Assay			IC50=12.3074 μM	SANGER
HOP-92	Growth Inhibition Assay			IC50=12.5233 μM	SANGER
M14	Growth Inhibition Assay			IC50=13.0335 μM	SANGER
SKG-IIIa	Growth Inhibition Assay			IC50=13.0554 μM	SANGER
Becker	Growth Inhibition Assay			IC50=13.058 μM	SANGER
ES5	Growth Inhibition Assay			IC50=13.1356 μM	SANGER
NCI-H2030	Growth Inhibition Assay			IC50=13.2062 μM	SANGER
SNU-423	Growth Inhibition Assay			IC50=13.381 μM	SANGER
CP50-MEL-B	Growth Inhibition Assay			IC50=13.6232 μM	SANGER
NCI-H1092	Growth Inhibition Assay			IC50=13.7475 μM	SANGER
8-MG-BA	Growth Inhibition Assay			IC50=13.7513 μM	SANGER
CAL-27	Growth Inhibition Assay			IC50=14.0727 μM	SANGER
AGS	Growth Inhibition Assay			IC50=14.1319 μM	SANGER
MZ2-MEL	Growth Inhibition Assay			IC50=14.1488 μM	SANGER
BFTC-909	Growth Inhibition Assay			IC50=14.5055 μM	SANGER
D-566MG	Growth Inhibition Assay			IC50=14.7706 μM	SANGER
MZ7-mel	Growth Inhibition Assay			IC50=15.1067 μM	SANGER
TE-5	Growth Inhibition Assay			IC50=15.2333 μM	SANGER
647-V	Growth Inhibition Assay			IC50=15.2705 μM	SANGER
DMS-114	Growth Inhibition Assay			IC50=15.3291 μM	SANGER
ME-180	Growth Inhibition Assay			IC50=15.4783 μM	SANGER
MS-1	Growth Inhibition Assay			IC50=15.5623 μM	SANGER
NUGC-3	Growth Inhibition Assay			IC50=15.6245 μM	SANGER
GB-1	Growth Inhibition Assay			IC50=15.6437 μM	SANGER
LOXIMVI	Growth Inhibition Assay			IC50=15.8919 μM	SANGER
NCI-H1304	Growth Inhibition Assay			IC50=15.8928 μM	SANGER
KURAMOCHI	Growth Inhibition Assay			IC50=15.971 μM	SANGER
SW1783	Growth Inhibition Assay			IC50=16.0993 μM	SANGER
EGI-1	Growth Inhibition Assay			IC50=16.5365 μM	SANGER
KGN	Growth Inhibition Assay			IC50=16.5586 μM	SANGER
PANC-03-27	Growth Inhibition Assay			IC50=16.7662 μM	SANGER
RH-1	Growth Inhibition Assay			IC50=16.825 μM	SANGER
HT-1376	Growth Inhibition Assay			IC50=16.8446 μM	SANGER
ZR-75-30	Growth Inhibition Assay			IC50=16.9361 μM	SANGER
TI-73	Growth Inhibition Assay			IC50=16.9408 μM	SANGER
TK10	Growth Inhibition Assay			IC50=17.0079 μM	SANGER
SW684	Growth Inhibition Assay			IC50=17.0154 μM	SANGER
RPMI-8866	Growth Inhibition Assay			IC50=17.0699 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay			IC50=17.5201 μM	SANGER
NCI-H2291	Growth Inhibition Assay			IC50=18.4345 μM	SANGER
T47D	Growth Inhibition Assay			IC50=18.488 μM	SANGER
A204	Growth Inhibition Assay			IC50=18.5452 μM	SANGER
HT-144	Growth Inhibition Assay			IC50=18.7427 μM	SANGER
MDA-MB-361	Growth Inhibition Assay			IC50=19.0714 μM	SANGER
HCC1806	Growth Inhibition Assay			IC50=19.1591 μM	SANGER
NCI-H596	Growth Inhibition Assay			IC50=19.6391 μM	SANGER
K-562	Growth Inhibition Assay			IC50=19.8926 μM	SANGER
D-336MG	Growth Inhibition Assay			IC50=19.9322 μM	SANGER
SK-MEL-2	Growth Inhibition Assay			IC50=20.1559 μM	SANGER
HEC-1	Growth Inhibition Assay			IC50=20.4978 μM	SANGER
EW-13	Growth Inhibition Assay			IC50=20.5443 μM	SANGER
Hs-578-T	Growth Inhibition Assay			IC50=20.5583 μM	SANGER
SJSA-1	Growth Inhibition Assay			IC50=20.5688 μM	SANGER
RCC10RGB	Growth Inhibition Assay			IC50=20.6719 μM	SANGER
LAN-6	Growth Inhibition Assay			IC50=21.0396 μM	SANGER
NCI-H1048	Growth Inhibition Assay			IC50=21.2258 μM	SANGER
CW-2	Growth Inhibition Assay			IC50=21.2705 μM	SANGER
HCC2157	Growth Inhibition Assay			IC50=21.36 μM	SANGER
EW-22	Growth Inhibition Assay			IC50=21.376 μM	SANGER
A253	Growth Inhibition Assay			IC50=21.4481 μM	SANGER
BE-13	Growth Inhibition Assay			IC50=21.6113 μM	SANGER
KINGS-1	Growth Inhibition Assay			IC50=21.6316 μM	SANGER
ETK-1	Growth Inhibition Assay			IC50=21.8152 μM	SANGER
NCI-H2452	Growth Inhibition Assay			IC50=21.8647 μM	SANGER
HPAF-II	Growth Inhibition Assay			IC50=21.8923 μM	SANGER
D-542MG	Growth Inhibition Assay			IC50=21.9069 μM	SANGER
NCI-H630	Growth Inhibition Assay			IC50=22.0296 μM	SANGER
TGBC11TKB	Growth Inhibition Assay			IC50=22.049 μM	SANGER
BCPAP	Growth Inhibition Assay			IC50=22.611 μM	SANGER
HLE	Growth Inhibition Assay			IC50=22.697 μM	SANGER
NCI-H727	Growth Inhibition Assay			IC50=22.735 μM	SANGER
RD	Growth Inhibition Assay			IC50=22.7518 μM	SANGER
NCI-H522	Growth Inhibition Assay			IC50=22.8431 μM	SANGER
CGTH-W-1	Growth Inhibition Assay			IC50=23.0641 μM	SANGER
SK-LU-1	Growth Inhibition Assay			IC50=23.4683 μM	SANGER
BB65-RCC	Growth Inhibition Assay			IC50=23.7863 μM	SANGER
HSC-3	Growth Inhibition Assay			IC50=23.8527 μM	SANGER
SK-MES-1	Growth Inhibition Assay			IC50=24.0161 μM	SANGER
DB	Growth Inhibition Assay			IC50=24.1028 μM	SANGER
HO-1-N-1	Growth Inhibition Assay			IC50=24.3093 μM	SANGER
MKN1	Growth Inhibition Assay			IC50=24.68 μM	SANGER
MN-60	Growth Inhibition Assay			IC50=24.8519 μM	SANGER
8505C	Growth Inhibition Assay			IC50=25.1845 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay			IC50=25.2208 μM	SANGER
BB30-HNC	Growth Inhibition Assay			IC50=25.2483 μM	SANGER
KM12	Growth Inhibition Assay			IC50=25.5807 μM	SANGER
NB13	Growth Inhibition Assay			IC50=25.5898 μM	SANGER
HCC2998	Growth Inhibition Assay			IC50=26.0283 μM	SANGER
U-118-MG	Growth Inhibition Assay			IC50=26.0808 μM	SANGER
NB10	Growth Inhibition Assay			IC50=26.234 μM	SANGER
SK-LMS-1	Growth Inhibition Assay			IC50=27.0704 μM	SANGER
SW1573	Growth Inhibition Assay			IC50=27.11 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay			IC50=27.1294 μM	SANGER
DK-MG	Growth Inhibition Assay			IC50=27.1817 μM	SANGER
RMG-I	Growth Inhibition Assay			IC50=27.2029 μM	SANGER
SW1088	Growth Inhibition Assay			IC50=27.5042 μM	SANGER
SK-OV-3	Growth Inhibition Assay			IC50=27.7593 μM	SANGER
KP-N-YN	Growth Inhibition Assay			IC50=27.8043 μM	SANGER
RPMI-7951	Growth Inhibition Assay			IC50=28.2456 μM	SANGER
CaR-1	Growth Inhibition Assay			IC50=28.5582 μM	SANGER
MKN7	Growth Inhibition Assay			IC50=28.5964 μM	SANGER
CHP-212	Growth Inhibition Assay			IC50=28.7401 μM	SANGER
KYSE-450	Growth Inhibition Assay			IC50=28.8091 μM	SANGER
RXF393	Growth Inhibition Assay			IC50=29.2906 μM	SANGER
P30-OHK	Growth Inhibition Assay			IC50=29.3048 μM	SANGER
Ca9-22	Growth Inhibition Assay			IC50=29.4299 μM	SANGER
RT-112	Growth Inhibition Assay			IC50=29.4611 μM	SANGER
ES1	Growth Inhibition Assay			IC50=29.8544 μM	SANGER
HCE-4	Growth Inhibition Assay			IC50=29.8786 μM	SANGER
AM-38	Growth Inhibition Assay			IC50=30.0606 μM	SANGER
AsPC-1	Growth Inhibition Assay			IC50=30.8053 μM	SANGER
TE-9	Growth Inhibition Assay			IC50=30.8737 μM	SANGER
HuP-T4	Growth Inhibition Assay			IC50=30.9638 μM	SANGER
LB2518-MEL	Growth Inhibition Assay			IC50=31.0744 μM	SANGER
NCI-H1703	Growth Inhibition Assay			IC50=31.0905 μM	SANGER
NCI-H2228	Growth Inhibition Assay			IC50=31.4651 μM	SANGER
SCC-4	Growth Inhibition Assay			IC50=31.9181 μM	SANGER
KNS-81-FD	Growth Inhibition Assay			IC50=32.0128 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay			IC50=32.0739 μM	SANGER
Daoy	Growth Inhibition Assay			IC50=32.0838 μM	SANGER
ES4	Growth Inhibition Assay			IC50=32.3922 μM	SANGER
MHH-ES-1	Growth Inhibition Assay			IC50=32.4872 μM	SANGER
C-33-A	Growth Inhibition Assay			IC50=32.7728 μM	SANGER
BHY	Growth Inhibition Assay			IC50=32.9495 μM	SANGER
Detroit562	Growth Inhibition Assay			IC50=33.6657 μM	SANGER
EW-1	Growth Inhibition Assay			IC50=33.9322 μM	SANGER
NCI-H1395	Growth Inhibition Assay			IC50=34.0799 μM	SANGER
KM-H2	Growth Inhibition Assay			IC50=36.4743 μM	SANGER
NCI-H520	Growth Inhibition Assay			IC50=36.7541 μM	SANGER
D-392MG	Growth Inhibition Assay			IC50=37.3344 μM	SANGER
NOMO-1	Growth Inhibition Assay			IC50=37.8089 μM	SANGER
TE-6	Growth Inhibition Assay			IC50=38.124 μM	SANGER
HT-3	Growth Inhibition Assay			IC50=38.4436 μM	SANGER
RPMI-8226	Growth Inhibition Assay			IC50=38.9519 μM	SANGER
U251	Growth Inhibition Assay			IC50=39.0124 μM	SANGER
GCT	Growth Inhibition Assay			IC50=39.1852 μM	SANGER
A431	Growth Inhibition Assay			IC50=39.1894 μM	SANGER
TE-12	Growth Inhibition Assay			IC50=39.2448 μM	SANGER
HMV-II	Growth Inhibition Assay			IC50=39.5047 μM	SANGER
IST-MES1	Growth Inhibition Assay			IC50=40.1502 μM	SANGER
SW13	Growth Inhibition Assay			IC50=40.519 μM	SANGER
SF295	Growth Inhibition Assay			IC50=40.8066 μM	SANGER
MDA-MB-157	Growth Inhibition Assay			IC50=40.9462 μM	SANGER
TE-1	Growth Inhibition Assay			IC50=41.8714 μM	SANGER
HuCCT1	Growth Inhibition Assay			IC50=42.5289 μM	SANGER
KYSE-510	Growth Inhibition Assay			IC50=43.1754 μM	SANGER
NCI-H358	Growth Inhibition Assay			IC50=44.0304 μM	SANGER
SW900	Growth Inhibition Assay			IC50=44.0758 μM	SANGER
CAS-1	Growth Inhibition Assay			IC50=44.0991 μM	SANGER
TE-8	Growth Inhibition Assay			IC50=44.5658 μM	SANGER
P12-ICHIKAWA	Growth Inhibition Assay			IC50=44.6112 μM	SANGER
YKG-1	Growth Inhibition Assay			IC50=44.781 μM	SANGER
SNU-C2B	Growth Inhibition Assay			IC50=44.7903 μM	SANGER
LCLC-103H	Growth Inhibition Assay			IC50=44.8703 μM	SANGER
U-87-MG	Growth Inhibition Assay			IC50=45.1022 μM	SANGER
HAL-01	Growth Inhibition Assay			IC50=45.1379 μM	SANGER
OVCAR-8	Growth Inhibition Assay			IC50=45.3564 μM	SANGER
KYSE-150	Growth Inhibition Assay			IC50=47.1052 μM	SANGER
OE33	Growth Inhibition Assay			IC50=47.1429 μM	SANGER
CAL-62	Growth Inhibition Assay			IC50=47.4068 μM	SANGER
TCCSUP	Growth Inhibition Assay			IC50=47.6264 μM	SANGER
H4	Growth Inhibition Assay			IC50=47.6621 μM	SANGER
CP66-MEL	Growth Inhibition Assay			IC50=48.0704 μM	SANGER
SNB75	Growth Inhibition Assay			IC50=48.1874 μM	SANGER
Mo-T	Growth Inhibition Assay			IC50=48.279 μM	SANGER
BT-549	Growth Inhibition Assay			IC50=48.3729 μM	SANGER
IGR-1	Growth Inhibition Assay			IC50=48.6645 μM	SANGER
NCI-H1299	Growth Inhibition Assay			IC50=48.7248 μM	SANGER
ALL-PO	Growth Inhibition Assay			IC50=49.9268 μM	SANGER
CLL	Function assay			Inhibition of DNA-PK autophosphorylation at Ser2056 residue in human CLL cells by Western blot method, IC50 = 0.03 μM.	22835870
HeLa	Function assay			Inhibition of DNA-PK autophosphorylation at S2056 in human HeLa cells, EC50 = 0.212 μM.	23855836
CHO	Function assay			Inhibition of human ERG expressed in cisapride treated CHO cells by Ion Works assay, IC50 = 14 μM.	23855836
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
Saos-2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
RD	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells	29435139
BT-12	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
MG 63 (6-TG R)	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
OHS-50	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
SJ-GBM2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
Rh18	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells	29435139
INA-6	Function assay	10 uM	4 hrs	Inhibition of heat shock response in human INA-6 cells assessed as reduction in HSF-1 mediated upregulation of the HSP72 protein expression at 10 uM preincubated for 4 hrs followed by HSP90 inhibitor and HSR inducer NVP-AUY922 addition measured after 4 hr	28453931
INA-6	Function assay	10 uM	4 hrs	Inhibition of heat shock response in human INA-6 cells assessed as reduction in HSF-1 mediated upregulation of the HSP72 mRNA expression at 10 uM preincubated for 4 hrs followed by HSP90 inhibitor and HSR inducer NVP-AUY922 addition measured after 4 hrs b	28453931
INA-6	Function assay	10 uM	4 hrs	Downregulation of NVP-AUY922-induced HSF-1 protein expression in human INA-6 cells at 10 uM preincubated for 4 hrs followed by NVP-AUY922 addition measured after 4 hrs by Western blot method	28453931
INA-6	Function assay	10 uM	4 hrs	Inhibition of NVP-AUY922-induced HSF-1 phosphorylation in human INA-6 cells at 10 uM preincubated for 4 hrs followed by NVP-AUY922 addition measured after 4 hrs by Western blot method	28453931
INA-6	Function assay	10 uM	4 hrs	Downregulation of NVP-AUY922-induced HSF-1 mRNA expression in human INA-6 cells at 10 uM preincubated for 4 hrs followed by NVP-AUY922 addition measured after 4 hrs by RT-PCR method	28453931
Click to View More Cell Line Experimental Data

Biological Activity

Description

Targets

DNA-PK ^[1] (Cell-free assay)	mTOR ^[1] (cell-free assay)	PI3K ^[1] (cell-free assay)
14 nM	1.7 μM	5 μM

In vitro
In vitro	NU7441 increases the persistence of γH2AX foci after ionizing radiation–induced DNA damage. NU7441 (0.5 μM or 1 μM) appreciably increases G2-M accumulation induced by ionizing radiation, and doxorubicin in both SW620 and LoVo cells. ^[2] NU7441 causes persistence of doxorubicin- and ionising radiation-induced DNA double-strand break and also slightly decreases homologous recombination activity DNA-PK-proficient M059-Fus-1 and DNA-PK-deficient M059 J human tumour cells. ^[3] NU7441 inhibits UV-induced RPA p34 hyperphosphorylation in a dose-dependent manner both in cells lacking and cells expressing polymerase η. ^[4] NU7441 increases levels of -induced γH2AX foci and correspondingly decreased -induced cell death in chronic lymphocytic leukemia cells. ^[5] NU7441 also inhibits -induced DNA-PKcs autophosphorylation and repair in chronic lymphocytic leukemia cells. ^[6] It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage^[7].
Cell Research	Cell lines	SW620, LoVo, V3-YAC and V3 cells
	Concentrations	0.5 μM or 1 μM
	Incubation Time	17 hours
	Method	The effect of NU7441 on cellular survival following exposure to doxorubicin, and ionizing radiation is measured in SW620, LoVo, V3, and V3-YAC cells by clonogenic assays. Briefly, growing cells in six-well plates or 6-cm dishes are exposed to doxorubicin with or without NU7441 (0.5 or 1.0 μM) for 16 hours. For radiosensitization studies, NU7441 is added to the cells 1 hour before irradiation. V3 and V3-YAC cells are exposed to γ-irradiation (3.1 Gy/min ¹³⁷Cesium). SW620 and LoVo are exposed to X-irradiation (2.9 Gy/min at 230 kV, 10 mA) due to the equipment available. After irradiation, the cells are incubated with or without NU7441 for a further 16 hours. Cells are then harvested by trypsinization, counted, and seeded into 10-cm diameter Petri dishes at densities varying from 100 to 10⁵ per dish in drug-free medium for colony formation. Colonies are stained with crystal violet after 10 to 14 days and counted with an automated colony counter. The survival reduction factor (SRF) is calculated as the surviving fraction of cells in the absence of NU7441 divided by the surviving fraction of cells in the presence of NU7441 for any given dose or concentration of cytotoxic agent. The dose modification ratio (DMR90) is calculated as the concentration/dose of cytotoxic agent required to kill 90% of the cells in the absence of NU7441 divided by the concentration/dose of cytotoxic agent required to kill 90% of the cells in the presence of NU7441.
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51 p-mTOR(Ser2448) / mTOR / p-S6K(Thr389)		29344644

In Vivo
In vivo	NU7441 intraperitoneally administrated at dose of 10 mg/kg maintains for at least 4 hours shows nontoxic and increases induced tumor growth delay 2-fold in mice bearing SW620 xenografts. ^[2]
Animal Research	Animal Models	Female rude mice bearing SW620 xenografts
	Dosages	10 mg/kg
	Administration	Intraperitoneally administrated

References

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Chemical Information & Solubility

Molecular Weight	413.49	Formula	C₂₅H₁₉NO₃S
CAS No.	503468-95-9	SDF	Download NU7441 (KU-57788) SDF
Smiles	C1COCCN1C2=CC(=O)C3=C(O2)C(=CC=C3)C4=CC=CC5=C4SC6=CC=CC=C56
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 5 mg/mL ( (12.09 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.750mg/ml (1.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.500mg/ml (1.21mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Could you please suggest formulations of the drug for in vivo injection?

Answer:
We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

Question 2:
Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

Answer:
Slight color variation due to different processing is normal and usually doesn't interfere with the function.

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