NU7441 (KU-57788)

Catalog No.S2638

For research use only.

NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.

NU7441 (KU-57788) Chemical Structure

CAS No. 503468-95-9

Selleck's NU7441 (KU-57788) has been cited by 196 publications

Purity & Quality Control

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Biological Activity

Description NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.
Targets
DNA-PK [1]
(Cell-free assay)
mTOR [1]
(cell-free assay)
PI3K [1]
(cell-free assay)
14 nM 1.7 μM 5 μM
In vitro

NU7441 increases the persistence of γH2AX foci after ionizing radiation–induced or etoposide-induced DNA damage. NU7441 (0.5 μM or 1 μM) appreciably increases G2-M accumulation induced by ionizing radiation, etoposide, and doxorubicin in both SW620 and LoVo cells. [2] NU7441 causes persistence of doxorubicin- and ionising radiation-induced DNA double-strand break and also slightly decreases homologous recombination activity DNA-PK-proficient M059-Fus-1 and DNA-PK-deficient M059 J human tumour cells. [3] NU7441 inhibits UV-induced RPA p34 hyperphosphorylation in a dose-dependent manner both in cells lacking and cells expressing polymerase η. [4] NU7441 increases levels of fludarabine-induced γH2AX foci and correspondingly decreased fludarabine-induced cell death in chronic lymphocytic leukemia cells. [5] NU7441 also inhibits mitoxantrone-induced DNA-PKcs autophosphorylation and repair in chronic lymphocytic leukemia cells. [6] It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage[7].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1770 NH;DNmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwMEKxOFMh|ryP Mlj0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
A172 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\3PXVKSzVyPUCuNVU{OTZizszN NH\ueYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NCI-H526 M1\LXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFu0V|ZKSzVyPUCuNlg1ODdizszN MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
HDLM-2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jBUGlEPTB;MD6zNlM1PSEQvF2= M1TteFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
HuO-3N1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjRZ5ZKSzVyPUCuOFY3OzZizszN NGXJWIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NCI-SNU-1 M{jxRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HyOGlEPTB;MD62NFk6PCEQvF2= NH\iZVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
SK-N-AS NX;3R29uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfvfHBKSzVyPUCuOlIzOTZizszN MoXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
LoVo NX;0eJdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULyTFJYUUN3ME2wMlg3PzR7IN88US=> MnLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
LAMA-84 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DRNWlEPTB;MD65PFg{OyEQvF2= NInE[GQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
CTB-1 M4[1[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LzeWlEPTB;MT6xNVU1PiEQvF2= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
KP-N-YS NXvEVZBCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzucWFKSzVyPUGuNVQzOzFizszN M4PwV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
HH MnPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTFwMUiwPVMh|ryP NXL6NXZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
CHL-1 NGHzXFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TqU2lEPTB;MT6yNVM3QCEQvF2= MnzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NB7 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnTepFKSzVyPUGuNlQ4PDVizszN NWnwNWNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
HL-60 M{XCfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nIRWlEPTB;MT6zPFE6KM7:TR?= MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
639-V NWPDWJJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHLWoZKSzVyPUGuOFQyOjhizszN M2G4N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
NCI-H1793 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzjTWM2OD1zLkS1PFQh|ryP NFzVR5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NEC8 NGTnXm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfCTWM2OD1zLkW1N|Uh|ryP NInGc5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
PA-1 NGHacHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PDfmlEPTB;MT61PFI6PCEQvF2= MnvxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
D-423MG M{fRfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTFwNkmzOlEh|ryP NYSyc4VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NCI-H292 NIflPJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYro[WRbUUN3ME2xMlc1ODF|IN88US=> M13LOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
SK-MEL-1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTFwN{m0O|kh|ryP MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
LB2241-RCC NVK2fJc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4P0PWlEPTB;Mj6xOFE1PiEQvF2= MnXKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
SK-MEL-3 NWrsUWRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTJwMU[0PUDPxE1? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
MHH-PREB-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTJwMU[2NFkh|ryP MlfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
BFTC-905 Mn7oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnT3TWM2OD1{LkG2Olg4KM7:TR?= NYq5XI1rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkL5TWM2OD1{LkG5PVA6KM7:TR?= NH\QRlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
G-361 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTJwMkG2PEDPxE1? M4i0eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
MOLT-4 Ml3HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTWcGEzUUN3ME2yMlIyQDh6IN88US=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
DEL M2jX[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTJwM{W1OlQh|ryP MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
MV-4-11 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4G3[GlEPTB;Mj6zO|M6KM7:TR?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-NEP-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHpTWM2OD1{LkO4O|g{KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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HuH-7 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTJwNU[3NFIh|ryP MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
A101D NID1XodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorvTWM2OD1{Lk[xN|M5KM7:TR?= NXnHWHU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
KG-1 MnfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DRXWlEPTB;Mj62O|kzPCEQvF2= MkO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
KALS-1 MluyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXG5OYxnUUN3ME2yMlY6PjN|IN88US=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
VMRC-RCZ MlvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHnTWM2OD1{Lke3N|I5KM7:TR?= M4PKdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
SK-UT-1 NEjITFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2WxdWlEPTB;Mz6xOVMyKM7:TR?= M1jOZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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DBTRG-05MG MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTNwMkiwPFUh|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
PANC-08-13 NVTUTWNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTNwNEGyO|gh|ryP NVLVbnlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
QIMR-WIL MkH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfmTWM2OD1|LkSxN|QyKM7:TR?= NHW3[4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
SK-MEL-30 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HSOGlEPTB;Mz60NlU6QCEQvF2= NU\td3VyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
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NCI-H2291 M37vN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDpclVKSzVyPUG4MlQ{PDVizszN NXuz[VhSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
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HT-144 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTEbGxJUUN3ME2xPE44PDJ5IN88US=> MlrGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
MDA-MB-361 M1S0[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfiV4NKSzVyPUG5MlA4OTRizszN NWLsO4llRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
HCC1806 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7CWVFlUUN3ME2xPU4yPTlzIN88US=> NEXz[XY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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D-336MG M17zdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLpTWM2OD1zOT65N|IzKM7:TR?= NXzNbW1nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
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RCC10RGB MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYf5TotXUUN3ME2yNE43PzF7IN88US=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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CW-2 NHvpe4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XDfWlEPTB;MkGuNlcxPSEQvF2= Mn3WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
HCC2157 NED2b3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;tTWM2OD1{MT6zOkDPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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KINGS-1 NEnkTndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTJzLk[zNVYh|ryP M{fkNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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NCI-H2452 NHzjZWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfaO5p6UUN3ME2yNU45PjR5IN88US=> M3fRSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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NCI-H630 M3naWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3icI45UUN3ME2yNk4xOjl4IN88US=> NEXvW449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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NCI-H727 MkS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojxTWM2OD1{Mj63N|Uh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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NCI-H522 Mm\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXyd49CUUN3ME2yNk45PDNzIN88US=> NH3nfW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
CGTH-W-1 MmnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTJ|LkC2OFEh|ryP M4C5[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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BB65-RCC MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7s[HVKSzVyPUKzMlc5PjNizszN NFOwWms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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BT-12 MWDxTHRUKGG|c3H5 MofYdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy| NH;CeZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MXLxTHRUKGG|c3H5 MWXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? MkPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 M2[yPJFJXFNiYYPzZZk> M{\RXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= Mlj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 NHPmTlZyUFSVIHHzd4F6 MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| M3Lwc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 M1;yO5FJXFNiYYPzZZk> NHvaXFByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> MkDBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MVHxTHRUKGG|c3H5 NVvqZ2tkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> Ml;RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 NU\lcnhzeUiWUzDhd5NigQ>? MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= NW\ndWdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 NFjEbm1yUFSVIHHzd4F6 MWnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 M1XJU5FJXFNiYYPzZZk> NY\2Z|RQeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoNVgh[2WubIO= Mmj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
INA-6 MYHGeY5kfGmxbjDhd5NigQ>? NWTme285OTBidV2= NIO3PVk1KGi{cx?= MoDwTY5pcWKrdHnvckBw\iCqZXH0JJNpd2OtIILld5BwdnOnIHnuJIh2dWGwIFnORU03KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDIV2YuOSCvZXTpZZRm\CC3cILl[5Vt[XSrb36gc4YhfGinIFjTVFczKHC{b4TlbY4h\XiycnXzd4lwdiCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KEiVUEmwJIlvcGmkaYTvdkBidmRiSGPSJIlv\HWlZYKgUnZRNUGXWUmyNkBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjC0JIhz MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-
INA-6 MWXGeY5kfGmxbjDhd5NigQ>? M13BUFExKHWP MVy0JIhzew>? Mn7nTY5pcWKrdHnvckBw\iCqZXH0JJNpd2OtIILld5BwdnOnIHnuJIh2dWGwIFnORU03KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDIV2YuOSCvZXTpZZRm\CC3cILl[5Vt[XSrb36gc4YhfGinIFjTVFczKG2UTlGg[ZhxemW|c3nvckBifCBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JGhUWDlyIHnubIljcXSxcjDhcoQhUFOUIHnu[JVk\XJiTm\QMWFWYTl{MjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB2IHjyd{Bj NFz1cIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES1N|k{OSd-Mki0OVM6OzF:L3G+
INA-6 NEPBUpVHfW6ldHnvckBie3OjeR?= Mn;XNVAhfU1? NXe4PHhUPCCqcoO= MkjHSI94dnKnZ4XsZZRqd25ib3[gUnZRNUGXWUmyNk1qdmS3Y3XkJGhUTi1zIIDyc5RmcW5iZYjwdoV{e2mxbjDpckBpfW2jbjDJUmEuPiClZXzsd{BifCBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JG5XWC2DVWm5NlIh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUO5N|EoRjJ6NEWzPVMyRC:jPh?=
INA-6 MX\GeY5kfGmxbjDhd5NigQ>? MYGxNEB2VQ>? MkjnOEBpenN? M3r3cGlvcGmkaYTpc44hd2ZiTm\QMWFWYTl{Mj3pcoR2[2WmIFjTSk0yKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBKVkFvNjDj[YxteyCjdDCxNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KE6YUD3BWXk6OjJiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? NXzre|U2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OVM6OzFpPkK4OFU{QTNzPD;hQi=>
INA-6 NVmxWYd7TnWwY4Tpc44h[XO|YYm= MnzNNVAhfU1? M1\4TlQhcHK| NE\BSYFFd3ewcnXneYxifGmxbjDv[kBPXlBvQWXZPVIzNWmwZIXj[YQhUFOILUGgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIFnORU03KGOnbHzzJIF1KDFyIIXNJJBz\WmwY4XiZZRm\CCob4KgOEBpenNiZn;scI94\WRiYomgUnZRNUGXWUmyNkBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjC0JIhzeyCkeTDSWE1RS1JibXX0bI9l MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-
Assay
Methods Test Index PMID
Western blot p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51 ; p-mTOR(Ser2448) / mTOR / p-S6K(Thr389) 29344644 25714019
In vivo NU7441 intraperitoneally administrated at dose of 10 mg/kg maintains for at least 4 hours shows nontoxic and increases etoposide-induced tumor growth delay 2-fold in mice bearing SW620 xenografts. [2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: SW620, LoVo, V3-YAC and V3 cells
  • Concentrations: 0.5 μM or 1 μM
  • Incubation Time: 17 hours
  • Method:

    The effect of NU7441 on cellular survival following exposure to etoposide, doxorubicin, and ionizing radiation is measured in SW620, LoVo, V3, and V3-YAC cells by clonogenic assays. Briefly, growing cells in six-well plates or 6-cm dishes are exposed to etoposide or doxorubicin with or without NU7441 (0.5 or 1.0 μM) for 16 hours. For radiosensitization studies, NU7441 is added to the cells 1 hour before irradiation. V3 and V3-YAC cells are exposed to γ-irradiation (3.1 Gy/min 137Cesium). SW620 and LoVo are exposed to X-irradiation (2.9 Gy/min at 230 kV, 10 mA) due to the equipment available. After irradiation, the cells are incubated with or without NU7441 for a further 16 hours. Cells are then harvested by trypsinization, counted, and seeded into 10-cm diameter Petri dishes at densities varying from 100 to 105 per dish in drug-free medium for colony formation. Colonies are stained with crystal violet after 10 to 14 days and counted with an automated colony counter. The survival reduction factor (SRF) is calculated as the surviving fraction of cells in the absence of NU7441 divided by the surviving fraction of cells in the presence of NU7441 for any given dose or concentration of cytotoxic agent. The dose modification ratio (DMR90) is calculated as the concentration/dose of cytotoxic agent required to kill 90% of the cells in the absence of NU7441 divided by the concentration/dose of cytotoxic agent required to kill 90% of the cells in the presence of NU7441.

Animal Research:

[2]

  • Animal Models: Female rude mice bearing SW620 xenografts
  • Dosages: 10 mg/kg
  • Administration: Intraperitoneally administrated

Solubility (25°C)

In vitro

DMSO 4 mg/mL warmed
(9.67 mM)
Water Insoluble
Ethanol Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.

0.5mg/mL

Chemical Information

Molecular Weight 413.49
Formula

C25H19NO3S

CAS No. 503468-95-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1COCCN1C2=CC(=O)C3=C(O2)C(=CC=C3)C4=CC=CC5=C4SC6=CC=CC=C56

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
Could you please suggest formulations of the drug for in vivo injection?

Answer:
We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

Question 2:
Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

Answer:
Slight color variation due to different processing is normal and usually doesn't interfere with the function.

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