NU7441 (KU-57788)

For research use only.

Catalog No.S2638

156 publications

NU7441 (KU-57788) Chemical Structure

CAS No. 503468-95-9

NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.

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Selleck's NU7441 (KU-57788) has been cited by 156 publications

Purity & Quality Control

Choose Selective DNA-PK Inhibitors

Biological Activity

Description NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.
Targets
DNA-PK [1]
(Cell-free assay)
mTOR [1]
(cell-free assay)
PI3K [1]
(cell-free assay)
14 nM 1.7 μM 5 μM
In vitro

NU7441 increases the persistence of γH2AX foci after ionizing radiation–induced or etoposide-induced DNA damage. NU7441 (0.5 μM or 1 μM) appreciably increases G2-M accumulation induced by ionizing radiation, etoposide, and doxorubicin in both SW620 and LoVo cells. [2] NU7441 causes persistence of doxorubicin- and ionising radiation-induced DNA double-strand break and also slightly decreases homologous recombination activity DNA-PK-proficient M059-Fus-1 and DNA-PK-deficient M059 J human tumour cells. [3] NU7441 inhibits UV-induced RPA p34 hyperphosphorylation in a dose-dependent manner both in cells lacking and cells expressing polymerase η. [4] NU7441 increases levels of fludarabine-induced γH2AX foci and correspondingly decreased fludarabine-induced cell death in chronic lymphocytic leukemia cells. [5] NU7441 also inhibits mitoxantrone-induced DNA-PKcs autophosphorylation and repair in chronic lymphocytic leukemia cells. [6] It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage[7].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1770 MlKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHi4eIVKSzVyPUCuNFIyPDNizszN M4PzOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
A172 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkj5TWM2OD1yLkG1N|E3KM7:TR?= NWrkeJJLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NCI-H526 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3n2emlEPTB;MD6yPFQxPyEQvF2= M3SxSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
HDLM-2 M4\IXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nVd2lEPTB;MD6zNlM1PSEQvF2= MkLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
HuO-3N1 NGDBcYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TK[2lEPTB;MD60OlY{PiEQvF2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTBwNkC5PVQh|ryP NHr3[Yc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
SK-N-AS MoPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTBwNkKyNVYh|ryP MlLEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
LoVo MkjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwOE[3OFkh|ryP NUfzT49GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
LAMA-84 M{LiVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DMdmlEPTB;MD65PFg{OyEQvF2= NG\kWGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
CTB-1 M{PEXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\KTWM2OD1zLkGxOVQ3KM7:TR?= M4PXXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
KP-N-YS NUfHO4IzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTFwMUSyN|Eh|ryP NXTHRoRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
HH NYGyOZZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\kPFJKSzVyPUGuNVgxQTNizszN NIPwdZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
CHL-1 NILkfopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXKTWM2OD1zLkKxN|Y5KM7:TR?= NGXxdZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NB7 MonoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTFwMkS3OFUh|ryP NEfBNWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
HL-60 M2jq[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXsTWM2OD1zLkO4NVkh|ryP M2HnXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
639-V M2PZeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHSTWM2OD1zLkS0NVI5KM7:TR?= NE\6TVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
NCI-H1793 NWH4UJduT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPYPZdKUUN3ME2xMlQ2QDRizszN NW\afnlYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
NEC8 NVTvcpdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFwNUWzOUDPxE1? NV;T[VM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
PA-1 MoTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTKTWM2OD1zLkW4Nlk1KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
D-423MG NXj0WlNHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILqcVFKSzVyPUGuOlk{PjFizszN MkLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
NCI-H292 NUPyNFNmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTFwN{SwNVMh|ryP MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-MEL-1 M3vyVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfGTWM2OD1zLke5OFc6KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
LB2241-RCC NVTLNlZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;2d2l3UUN3ME2yMlE1OTR4IN88US=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
SK-MEL-3 NVPsV4pjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jZVGlEPTB;Mj6xOlQ6KM7:TR?= NIDIOVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
MHH-PREB-1 NF3TOHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXQTWM2OD1{LkG2OlA6KM7:TR?= MljVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
BFTC-905 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPLNYlKUUN3ME2yMlE3Pjh5IN88US=> MmDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
Ramos-2G6-4C10 NX61RlNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHKwcoJKSzVyPUKuNVk6ODlizszN M4Tt[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
G-361 M1PpdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mly0TWM2OD1{LkKxOlgh|ryP NFPLZ3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
MOLT-4 M3PjR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTJwMkG4PFgh|ryP NHL0TXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
DEL M{LM[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPQTWM2OD1{LkO1OVY1KM7:TR?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
MV-4-11 Ml;uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVr4cYJ3UUN3ME2yMlM4OzlizszN MmO3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
SK-NEP-1 NGrmU4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnqwTWM2OD1{LkO4O|g{KM7:TR?= NEP5fYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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A101D M1;2Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPkRm1KSzVyPUKuOlE{OzhizszN NF\1Umw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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KALS-1 NHLuZXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4q2Z2lEPTB;Mj62PVY{OyEQvF2= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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DBTRG-05MG MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrITWM2OD1|LkK4NFg2KM7:TR?= NUfVXnZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
PANC-08-13 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3EWmU{UUN3ME2zMlQyOjd6IN88US=> MnLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
QIMR-WIL MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTNwNEGzOFEh|ryP NWTtWlJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
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CAL-54 MmPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWS2S414UUN3ME2xNU41QDFzIN88US=> NX3iTYlCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
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FTC-133 NUL0ZYNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUK0UHU2UUN3ME2xNk4xODd4IN88US=> M1:0blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
D-283MED NWfBWIhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV3CUpZQUUN3ME2xNk4xPTZ7IN88US=> NHW5WGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDh4N{ivK|5USU6JRWK8M4E,
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BALL-1 Mon5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjSPIxzUUN3ME2xNk4zPjZ{IN88US=> M2rFR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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SKG-IIIa M2HKSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXlbWxsUUN3ME2xN{4xPTV2IN88US=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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NCI-H1092 M2GweGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnrTWM2OD1zMz63OFc2KM7:TR?= M1nIZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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CAL-27 NYP5Z3gyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\xZ3VGUUN3ME2xOE4xPzJ5IN88US=> NU\xOnJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh6Nke4M{c,W0GQR1XSQE9iRg>?
AGS Mny4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NED1PIZKSzVyPUG0MlE{OTlizszN MoDUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVg5Pjd6Lze+V2FPT0WUPD;hQi=>
MZ2-MEL MmrjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzxTWM2OD1zND6xOFg5KM7:TR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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MZ7-mel NVruNYVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLYTWM2OD1zNT6xNFY4KM7:TR?= M2H0e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5QDZ5OD:nQnNCVkeHUkyvZV4>
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GB-1 MnjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mln6TWM2OD1zNT62OFM4KM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFg3PzhxJ{7TRW5ITVJ:L3G+
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HeLa M2\n[GZ2dmO2aX;uJIF{e2G7 NYPRcHNoUW6qaXLpeIlwdiCxZjDEUmEuWEtiYYX0c5Bpd3OyaH;yfYxifGmxbjDheEBUOjB3NjDpckBpfW2jbjDI[WxiKGOnbHzzMEBGSzVyIE2gNE4zOTJizszNMi=> NH\lbWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1OVg{Pid-MkO4OVU5OzZ:L3G+
CHO MV;GeY5kfGmxbjDhd5NigQ>? NVrtcJB3UW6qaXLpeIlwdiCxZjDoeY1idiCHUleg[ZhxemW|c3XkJIlvKGOrc3Hwdoll\SC2cnXheIVlKEOKTzDj[YxteyCkeTDJc44hX2:{a4OgZZN{[XluIFnDOVAhRSBzNDFOwG0v NEH1fpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1OVg{Pid-MkO4OVU5OzZ:L3G+
A673 MVzxTHRUKGG|c3H5 MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NEToTVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY MmXCdWhVWyCjc4PhfS=> NED5c|FyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= MkCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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BT-37 MlPZdWhVWyCjc4PhfS=> MmXidWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy| NVnHc3U4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NEi0bVJyUFSVIHHzd4F6 MmnQdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ M17iTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 MnS0dWhVWyCjc4PhfS=> MkmwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy| MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) MWjxTHRUKGG|c3H5 NV;MN25DeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz NUTo[I5NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NYfENHdneUiWUzDhd5NigQ>? NHjpfFJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz M{D5NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 Mm[4dWhVWyCjc4PhfS=> M3W3UZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= NETLemw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 NXHNT4FueUiWUzDhd5NigQ>? MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NITwOWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC M37HOJFJXFNiYYPzZZk> Mn;wdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= NHjrXI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 Ml3qdWhVWyCjc4PhfS=> MXXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= M2LLXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 MoDydWhVWyCjc4PhfS=> M1rpN5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 M3j0RpFJXFNiYYPzZZk> MXjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
INA-6 MlrOSpVv[3Srb36gZZN{[Xl? NVHWdpZzOTBidV2= MkfWOEBpenN? NF;YPGdKdmirYnn0bY9vKG:oIHjlZZQhe2ixY3ugdoV{eG:wc3WgbY4hcHWvYX6gTW5CNTZiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGhUTi1zIH3l[IlifGWmIIXwdoVofWyjdHnvckBw\iC2aHWgTHNRPzJicILveIVqdiCneIDy[ZN{cW:wIHH0JFExKHWPIIDy[Ylv[3WkYYTl[EBnd3JiNDDodpMh\m:ubH;3[YQh[nliSGPQPVAhcW6qaXLpeI9zKGGwZDDIV3IhcW6mdXPldkBPXlBvQWXZPVIzKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHJ? MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUO5N|EoRjJ6NEWzPVMyRC:jPh?=
INA-6 NFPFbFJHfW6ldHnvckBie3OjeR?= M4fwTVExKHWP NV7SfVlRPCCqcoO= MYjJcohq[mm2aX;uJI9nKGinYYSgd4hw[2ticnXzdI9ve2ViaX6gbJVu[W5iSV7BMVYh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFjTSk0yKG2nZHnheIVlKHWycnXneYxifGmxbjDv[kB1cGViSGPQO|IhdVKQQTDlfJBz\XO|aX;uJIF1KDFyIIXNJJBz\WmwY4XiZZRm\CCob4KgOEBpenNiZn;scI94\WRiYomgTHNRQTBiaX7obYJqfG:{IHHu[EBJW1JiaX7keYNmeiCQVmCtRXV[QTJ{IHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDRiaILzJII> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-
INA-6 Mn\WSpVv[3Srb36gZZN{[Xl? MnHXNVAhfU1? NH;QWWQ1KGi{cx?= M{\kSGRwf26{ZXf1cIF1cW:wIH;mJG5XWC2DVWm5NlIucW6mdXPl[EBJW0ZvMTDwdo91\WmwIHX4dJJme3Orb36gbY4hcHWvYX6gTW5CNTZiY3XscJMh[XRiMUCgeW0heHKnaX7jeYJifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBPXlBvQWXZPVIzKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3M{mzNUc,Ojh2NUO5N|E9N2F-
INA-6 NHnIVVNHfW6ldHnvckBie3OjeR?= M4DFTlExKHWP MnLBOEBpenN? MVvJcohq[mm2aX;uJI9nKE6YUD3BWXk6OjJvaX7keYNm\CCKU1[tNUBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTW5CNTZiY3XscJMh[XRiMUCgeW0heHKnaX7jeYJifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBPXlBvQWXZPVIzKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JHdme3Sncn6gZoxwfCCvZYToc4Q> M4nEO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEWzPVMyLz5{OES1N|k{OTxxYU6=
INA-6 NWrBfIN3TnWwY4Tpc44h[XO|YYm= NEPIVW4yOCC3TR?= NHm1Rmc1KGi{cx?= Moi1SI94dnKnZ4XsZZRqd25ib3[gUnZRNUGXWUmyNk1qdmS3Y3XkJGhUTi1zIH3SUmEh\XiycnXzd4lwdiCrbjDoeY1idiCLTlGtOkBk\WyuczDheEAyOCC3TTDwdoVqdmO3YnH0[YQh\m:{IESgbJJ{KG[xbHzve4VlKGK7IF7WVE1CXVl7MkKgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiNDDodpMh[nliUmStVGNTKG2ndHjv[C=> M17aWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEWzPVMyLz5{OES1N|k{OTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-DNA-PKcs / DNA-PKcs / p-ATM / ATM / p-ATR / ATR / Ku70 / p-CHK1 / p-CHK2/ p-AKT / RAD51; 

PubMed: 29344644     


SUNE1 cells were irradiated with 5 Gy in the presence or absence of NU7441 (2 µM). The expression of cell cycle checkpoint proteins and double strand break repair proteins was assayed by western blot analysis at 6 or 12 h post-IR.

p-mTOR(Ser2448) / mTOR / p-S6K(Thr389); 

PubMed: 25714019     


Phosphorylation of mTOR and p70S6K were detected by Western blot analysis 24 hours after treatment.

29344644 25714019
In vivo NU7441 intraperitoneally administrated at dose of 10 mg/kg maintains for at least 4 hours shows nontoxic and increases etoposide-induced tumor growth delay 2-fold in mice bearing SW620 xenografts. [2]

Protocol

Cell Research:

[2]

- Collapse
  • Cell lines: SW620, LoVo, V3-YAC and V3 cells
  • Concentrations: 0.5 μM or 1 μM
  • Incubation Time: 17 hours
  • Method:

    The effect of NU7441 on cellular survival following exposure to etoposide, doxorubicin, and ionizing radiation is measured in SW620, LoVo, V3, and V3-YAC cells by clonogenic assays. Briefly, growing cells in six-well plates or 6-cm dishes are exposed to etoposide or doxorubicin with or without NU7441 (0.5 or 1.0 μM) for 16 hours. For radiosensitization studies, NU7441 is added to the cells 1 hour before irradiation. V3 and V3-YAC cells are exposed to γ-irradiation (3.1 Gy/min 137Cesium). SW620 and LoVo are exposed to X-irradiation (2.9 Gy/min at 230 kV, 10 mA) due to the equipment available. After irradiation, the cells are incubated with or without NU7441 for a further 16 hours. Cells are then harvested by trypsinization, counted, and seeded into 10-cm diameter Petri dishes at densities varying from 100 to 105 per dish in drug-free medium for colony formation. Colonies are stained with crystal violet after 10 to 14 days and counted with an automated colony counter. The survival reduction factor (SRF) is calculated as the surviving fraction of cells in the absence of NU7441 divided by the surviving fraction of cells in the presence of NU7441 for any given dose or concentration of cytotoxic agent. The dose modification ratio (DMR90) is calculated as the concentration/dose of cytotoxic agent required to kill 90% of the cells in the absence of NU7441 divided by the concentration/dose of cytotoxic agent required to kill 90% of the cells in the presence of NU7441.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Female rude mice bearing SW620 xenografts
  • Dosages: 10 mg/kg
  • Administration: Intraperitoneally administrated
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 4 mg/mL warmed (9.67 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
For best results, use promptly after mixing.
0.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 413.49
Formula

C25H19NO3S

CAS No. 503468-95-9
Storage powder
in solvent
Synonyms N/A
Smiles C1COCCN1C2=CC(=O)C3=C(O2)C(=CC=C3)C4=CC=CC5=C4SC6=CC=CC=C56

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Could you please suggest formulations of the drug for in vivo injection?

  • Answer:

    We tested the following formulation for in vivo use, 4% DMSO+30% PEG 300+5% Tween 80+ddH2O up to 1mg/mL. It is a clear solution.

  • Question 2:

    Previous aliquots were clear and colorless while this time, it becomes yellow when the powder was dissolved in DMSO? Could you advice on this?

  • Answer:

    Slight color variation due to different processing is normal and usually doesn't interfere with the function.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID