For research use only.

Catalog No.S7162 Synonyms: Mitochondrial division inhibitor 1

34 publications

Mdivi-1 Chemical Structure

CAS No. 338967-87-6

Mdivi-1 (Mitochondrial division inhibitor 1) is a selective cell-permeable inhibitor of mitochondrial division DRP1 (dynamin-related GTPase) and mitochondrial division Dynamin I (Dnm1) with IC50 of 1-10 μM. Mdivi-1 attenuates mitophagy and enhances apoptosis.

Selleck's Mdivi-1 has been cited by 34 publications

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Choose Selective Dynamin Inhibitors

Biological Activity

Description Mdivi-1 (Mitochondrial division inhibitor 1) is a selective cell-permeable inhibitor of mitochondrial division DRP1 (dynamin-related GTPase) and mitochondrial division Dynamin I (Dnm1) with IC50 of 1-10 μM. Mdivi-1 attenuates mitophagy and enhances apoptosis.
Features The first selective inhibitor of mitochondrial division dynamins.
Dnm1 GTPase [1]
(Cell-free assay)
1 μM-10 μM
In vitro

Mdivi-1 is a cell-permeable quinazolinone compound that inhibits yeast (Dnm1) and mammalian (Drp1) division DRPs (dynamin-related GTPases) and effectively induces mitochondrial fusion into net-like structures in a reversible manner. Cell-free studies indicate that mdivi-1 blocks Dnm1 ATPase activity (IC50<10 μM) and self-assembly by an allosteric modulation-based mechanism. Mdivi-1 is shown to effectively suppress STS- as well as C8-Bid-induced MOMP (Mitochondrial Outer Membrane Permeabilization) in HeLa cultures and in cell-free murine liver mitochondria preparations, respectively, as assessed by cytochrome C release. In cells, mdivi-1 retards apoptosis by inhibiting mitochondrial outer membrane permeabilization. In principle, mivi-1 represents a class of therapeutics for stroke, myocardial infarction, and neurodegenerative diseases. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2/ADM cells NHTZSG1E\WyuII\pZYJqdGm2eTDhd5NigQ>? NXTaPG1pOTBizszN MUOxJIg> MXHt[Il3cS1zIIDy[ZRz\WG2bXXueEBqdmO{ZXHz[YQhSjWJMT3pcoR2[2WmIHHwc5B1d3OrczDhcoQh[2WubDDk[YF1cCCrbjDI[ZBIOi:DRF2gZ4VtdHN? NVvtcYt2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C4OVA2QDVpPkOwPFUxPTh3PD;hQi=>
R28 MXjGeY5kfGmxbjDhd5NigQ>? MkDqOUDPxE1? M17tdVIhcA>? NUDOOVEzdWm2b3Poc45lemmjIHnuJHIzQCClZXzsd{BxemW2cnXheIVlKHerdHigOUDPxE1iRILwNUBqdmirYnn0c5IhVWSrdnmtNUBz\XSjaX7l[EB1cGViYY\ldoFo\SCuZX7neIgh[W6mIHHwdIViemWmIHHzJIVtd26pYYTl[EB1fWK3bHHyMEB1cHKnYXStcIls\SCwZYT3c5JseyCjZoTldkBqenKjZHnheIlwdg>? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV|OE[yNUc,OzB3M{i2NlE9N2F-
INS-1E Mmm2SpVv[3Srb36gZZN{[Xl? NVjYSXVqPTBizszN NYfoPYlvOTJiaB?= M3fpTpRz\WG2bXXueEB4cXSqIF3kbZZqNTFic3nncolncWOjboTsfUBqdmirYnn0[YQhdWm2b3Poc45lemmjbDDmdoFodWWwdHH0bY9vKGGwZDDjcIViemy7IHnuZ5Jm[XOnZDD0bIUhfmmjYnnsbZR6d2[yYX7jdoVifGmlIHLleIEhUU6VLUHFJINmdGy|IIXu[IVzKGi7cH;4bYEh[2:wZHn0bY9vew>? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd4OEWxN{c,Ojl5Nki1NVM9N2F-
L1210 MkHXSpVv[3Srb36gZZN{[Xl? MmS1Nk42NCB3IHHu[EAyOCEQvF2= MmjUO|IhcA>? MV\N[Il3cS1zIDi1JOK2VSlic3nncolncWOjboTsfUBifHSnboXheIVlKDBwNDDt[{9tKG:oIHPpd5Bt[XSrbj3pcoR2[2WmIHPlcIwh\GWjdHigbY4hVDF{MUCgZ4VtdHNuIIfpeIghfGinIHX4Z4VxfGmxbjDv[kAzNjViYX7kJFExKML3TTDv[kBO\Gm4aT2x MmnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4N{e4NVQoRjJ6Nke3PFE1RC:jPh?=
SH-SY5Y NFTxSppHfW6ldHnvckBie3OjeR?= NIezfWcyOCEQvF2= M{SyVFMxKG2rbh?= NF7lR|JKdmirYnn0bY5oKES{cEGgdJJwfGWldIOgZYdicW6|dDDDVGYucW6mdXPl[EBqdnS{aX7zbYMh[XCxcITvd4l{KGK7IHLsc4NscW6pIFLhfEB1emGwc3zvZ4F1cW:w MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7OEK5OEc,OjZ3OUiyPVQ9N2F-

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-Chk1 / p-Chk2 / Chk1 / Chk2 / p-ATM / ATM ; 

PubMed: 24952704     

MDA-MB-231 cells were treated with cisplatin alone, mdivi-1 alone or the combination at the indicated concentrations for 4 h Western blot was then performed to detect the phosphorylation status of key proteins involved in DNA damage response.

COX-2 / p-Drp1 / Drp1 / Mfn2 / ABCG2 / Oct4 ; 

PubMed: 28435473     

CNE1 and CNE2 cells were treated with or without various concentrations of Mdivi-1 (0, 10, or 20 μM) for 24 h and the level of COX-2, p-Drp1(Ser616), p-Drp1(Ser637), t-Drp1, Mfn2, ABCG2, and Oct4 in CNE1 and CNE2 cells were measured by WB assay. 

24952704 28435473

PubMed: 25458053     

MDA-MB-231 cells were synchronized at prometaphase by incubation with nocodazole (100 ng/ml) for 16 h, and then released for the indicated time in fresh media in the presence of vehicle DMSO or 50 mM mdivi-1. DNA was then visualized by staining cells with DAPI (blue), and microtubules were visualized by staining cells with Alexa Fluor 555-conjugated anti-b-tubulin antibody (red). Scale bars: 5 μm.

Growth inhibition assay
Cell viability; 

PubMed: 24952704     

MDA-MB-231 cells were treated with increasing doses of mdivi-1 for 72 h The survival was assessed by MTS assay.


PubMed: 26032958     

A375 and SK-MEL-28 cells were treated with mDIVI-1 (0, 5, 10, 25, 50, 100 μM) for 48 hours before AnnexinV-FITC analysis. 

24952704 26032958
In vivo Drp1 and GFAP protein expression is significantly increased in the early neurodegenerative events of ischemic mouse retina. Mdivi-1 treatment blocks apoptotic cell death in ischemic retina, and significantly increases RGC survival at 2 weeks after ischemia. In the normal mouse retina, Drp1 is expressed in the ganglion cell layer (GCL) as well as the inner plexiform layer, the inner nuclear layer (INL), and the outer plexiform layer (OPL). In the GCL, Drp1 immunoreactivity is strong in RGCs. While Drp1 protein expression is increased in the GCL of vehicle-treated ischemic retina at 12 hours. Mdivi-1 treatment does not change this increase of Drp1 protein expression but significantly decreased GFAP protein expression. [2]


Animal Research:


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  • Animal Models: C57BL/6 mice
  • Dosages: 50 mg/kg
  • Administration: IP injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 70 mg/mL (198.17 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 353.22


CAS No. 338967-87-6
Storage powder
in solvent
Synonyms Mitochondrial division inhibitor 1
Smiles COC1=C(C=C(C(=C1)N2C(=O)C3=CC=CC=C3NC2=S)Cl)Cl

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID