Mdivi-1

Catalog No.S7162 Batch:S716207

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Technical Data

Formula

C15H10Cl2N2O2S
Molecular Weight 353.22 CAS No. 338967-87-6
Solubility (25°C)* In vitro DMSO 125 mg/mL (353.88 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 5.000mg/ml (14.16mM) Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.800mg/ml (2.26mM) Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Mdivi-1 (Mitochondrial division inhibitor 1) is a selective cell-permeable inhibitor of mitochondrial division DRP1 (dynamin-related GTPase) and mitochondrial division Dynamin I (Dnm1) with IC50 of 1-10 μM. Mdivi-1 attenuates mitophagy and enhances apoptosis.
Targets
Dnm1 GTPase [1]
(Cell-free assay)
1 μM-10 μM
In vitro

Mdivi-1 is a cell-permeable quinazolinone compound that inhibits yeast (Dnm1) and mammalian (Drp1) division DRPs (dynamin-related GTPases) and effectively induces mitochondrial fusion into net-like structures in a reversible manner. Cell-free studies indicate that mdivi-1 blocks Dnm1 ATPase activity (IC50<10 μM) and self-assembly by an allosteric modulation-based mechanism. Mdivi-1 is shown to effectively suppress STS- as well as C8-Bid-induced MOMP (Mitochondrial Outer Membrane Permeabilization) in HeLa cultures and in cell-free murine liver mitochondria preparations, respectively, as assessed by cytochrome C release. In cells, mdivi-1 retards apoptosis by inhibiting mitochondrial outer membrane permeabilization. In principle, mivi-1 represents a class of therapeutics for stroke, myocardial infarction, and neurodegenerative diseases. [1]
In vivo

Drp1 and GFAP protein expression is significantly increased in the early neurodegenerative events of ischemic mouse retina. Mdivi-1 treatment blocks apoptotic cell death in ischemic retina, and significantly increases RGC survival at 2 weeks after ischemia. In the normal mouse retina, Drp1 is expressed in the ganglion cell layer (GCL) as well as the inner plexiform layer, the inner nuclear layer (INL), and the outer plexiform layer (OPL). In the GCL, Drp1 immunoreactivity is strong in RGCs. While Drp1 protein expression is increased in the GCL of vehicle-treated ischemic retina at 12 hours. Mdivi-1 treatment does not change this increase of Drp1 protein expression but significantly decreased GFAP protein expression. [2]
Features The first selective inhibitor of mitochondrial division dynamins.

Protocol (from reference)

Animal Study:

[3]
  • Animal Models

    Male Sprague Dawley rats

  • Dosages

    3 mg/kg

  • Administration

    IP injection

References

  • https://pubmed.ncbi.nlm.nih.gov/18267088/
  • https://pubmed.ncbi.nlm.nih.gov/21372007/
  • https://pubmed.ncbi.nlm.nih.gov/27065821/

Customer Product Validation

<p>a Representative TUNEL/DAPI photomicrographs of ipsilateral cortex in different groups (scale bar = 100 μm).</p>

, , Neurochem Res, 2017, 42(5):1449-1458

Cell necroptosis were detected in cells transfected with PCYT1A siRNA or treated with mitophagy inhibitor Mdivi-1 (50 μm).

Data from [ , , Blood Cancer J, 2017, doi:10.1038/bcj.2017.61 ]

Mdivi-1 relaxes ET-1-induced constriction, and mdivi-1 pre-treatment inhibits ET-1-induced constriction in rat mesenteric arteries. (A) Mdi-vi-1 induced concentration-dependent relaxation in endothelium-intact rat mesenteric arteries pre-contracted with ET-1 (4 nM). The relaxation ratio of mdivi-1 was calculated by subtracting the relaxation ratio of corresponding control (DMSO). (B) Mdivi-1 (10 µM) pretreatment inhibited ET-1- induced vasoconstriction in endothelium-intact rat mesenteric arteries. **P<0.01 vs. Control. (C) Mdivi-1 induced vasorelaxation in endothelium-denuded rat mesenteric arteries pre-contracted with ET-1 (4 nM). The relaxation ratio of mdivi-1 was calculated by subtracting the relaxation ratio of corresponding control (DMSO). (D) Mdivi-1 (10 µM) pretreatment inhibited ET-1-induced constriction of rat mesenteric arteries with denuded endothelium. **P<0.01 vs. Control.

Data from [ , , Cell Physiol Biochem, 2017, 42(5):1802-1811 ]

Neurons were pretreated with kaempferol or Mdivi-1 and then subjected to 2 h of dimethyl succinate or OGD insult. (A) View of mitochondrial localization of HK-II with confocal scanning microscope (Green: HK-II; Red: MitoTracker Red CMXRos; Scale bars: 10 μm).

Data from [ , , Biochim Biophys Acta Mol Basis Dis, 2017, 1863(9):2307-2318 ]

Selleck's Mdivi-1 has been cited by 108 publications

Fine particulate matter exacerbates asthma by activating STC2-mediated mitophagy through METTL3/YTHDF2-dependent m6A methylation [ J Hazard Mater, 2025, 495:138854] PubMed: 40499413
DRP1 downregulation impairs mitophagy, driving mitochondrial ROS and SASP production in rheumatoid arthritis CD4+PD-1+T cells [ Redox Biol, 2025, 86:103818] PubMed: 40825269
Polystyrene microplastics exacerbate mitophagy through mitochondrial dysfunction in the duck lung [ J Nanobiotechnology, 2025, 23(1):437] PubMed: 40500713
Targeting LIF With Cyclovirobuxine D to Suppress Tumor Progression via LIF/p38MAPK/p62-Modulated Mitophagy in Hepatocellular Carcinoma [ MedComm (2020), 2025, 6(6):e70227] PubMed: 40416597
Ginsenoside F2-modified liposomes delivering FTY720 enhance glioblastoma targeting and antitumor activity via ferroptosis [ Phytomedicine, 2025, 144:156917] PubMed: 40480023
Gestational zearalenone causes fetal intrauterine growth restriction partially through deriving ROS-Drp1 mediated placental PANoptosis [ Ecotoxicol Environ Saf, 2025, 302:118636] PubMed: 40712548
Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation [ PLoS Pathog, 2025, 21(1):e1012872] PubMed: 39804926
Reactive Oxygen Species-Mediated TRPM2 Activation Facilitates Phagocytosis of Macrophages to Reverse Profibrotic Phenotype [ Liver Int, 2025, 45(10):e70341] PubMed: 40952323
Myeloid PGC1β attenuates high-fat-diet induced inflammation via mitochondrial fission/mtDNA/Nlrp3 pathway [ Biochim Biophys Acta Mol Basis Dis, 2025, 1871(1):167528] PubMed: 39366644
HEP14 treatment improves ovarian function in aged mice through mitophagy enhancement and oxidative stress reduction [ Commun Biol, 2025, 8(1):1141] PubMed: 40751000

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.