Savolitinib (AZD6094)

For research use only.

Catalog No.S7674 Synonyms: HMPL-504, Volitinib

9 publications

Savolitinib (AZD6094) Chemical Structure

CAS No. 1313725-88-0

Savolitinib (Volitinib, AZD6094, HMPL-504) is a novel, potent, and selective MET inhibitor currently in clinical development in various indications, including PRCC. The IC50 values of this compound for c-Met and p-Met are 5 nM and 3 nM, respectively. It shows exquisite selectivity for c-Met over 274 kinase.

Selleck's Savolitinib (AZD6094) has been cited by 9 publications

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Biological Activity

Description Savolitinib (Volitinib, AZD6094, HMPL-504) is a novel, potent, and selective MET inhibitor currently in clinical development in various indications, including PRCC. The IC50 values of this compound for c-Met and p-Met are 5 nM and 3 nM, respectively. It shows exquisite selectivity for c-Met over 274 kinase.
p-Met [1]
(Cell-free assay)
c-Met [1]
(Cell-free assay)
3 nM 5 nM
In vitro

Volitinib has exquisite kinase selectivity and excellent potency[1]. Volitinib displays a highly selective profile across a gastric cell line panel, potently inhibiting cell growth only in those lines with dysregulated cMET (EC50 values 0.6 nM/L-12.5 nM/L)[2]. Volitinib has high membrane permeability without efflux transport across Caco-2 cell monolayer and exhibits negligible P-gp inhibition (IC50 > 17 μM). Volitinib shows no significant reversible or mechanism-based CYP inhibition in human liver microsomes, and no induction of CYP1A2 and CYP3A4 in human hepatocytes[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H441 NFPhR5pHfW6ldHnvckBie3OjeR?= NIH5cI8yKGh? NUXRVnB[UW6qaXLpeIlwdiCxZjDjMW1mfCCjdYTvdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIF7DTU1JPDRzIHPlcIx{KG[xcjCxJIhzKGK7IFXMTXNCNCCLQ{WwQVAvODB|IN88US=> NW\VXHV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOFgzODlpPkK1NVQ5OjB7PD;hQi=>
NCI-H441 MoPmVJJwdGmoZYLheIlwdiCjc4PhfS=> MVi3NkBp MoPiRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFQ1OSClZXzsd{Bie3Onc4Pl[EBieyCKR1[tbY5lfWOnZDDwdo9tcW[ncnH0bY9vKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4xODZizszN MnPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNEiyNFkoRjJ3MUS4NlA6RC:jPh?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
pMET / MET / pAKT / AKT / pERK / ERK; 

PubMed: 27472392     

Immunoblot analysis of H1993 and EBC-1 cells treated with 100 nM savolitinib for the indicated times.

Growth inhibition assay
Cell viability; 

PubMed: 27472392     

MET inhibitor GI50 determination in H1993 and EBC-1 cells treated as indicated for five days. Data shown are the mean ± S.D. normalized to vehicle control.

In vivo In a mouse pharmacokinetic study (male ICR mice), the clearance of the compound is 4.28 L/(h·kg) and the half-time is 1.7 h. Despite its moderate oral bioavailability (F = 27.2%), the overall plasma exposure is much higher. Volitinib demonstrates dose-dependent tumor growth inhibition in a U87MG subcutaneous xenograft model[1]. Its treatment leads to pharmacodynamic modulation of c-MET signaling and potent tumor stasis in 3/3 cMET-dysregulated gastric cancer patient-derived tumor xenograft models, but has negligible activity in a gastric cancer control model[2]. Volitinib has moderate plasma protein binding rate (60%∼70% in rat, dog, and human; 40% in mouse; 80% in monkey) and exhibits wide distribution to different organs in rat, with high exposures in liver and kidney, very low in brain, spinal cord and testis compared to the plasma level. In PK studies in mouse, rat and dog, Volitinib shows the rapid oral absorption (Tmax<2.5 h) with high exposures and the acceptable bioavailability at 27.2%, 42.6% and 86.3%, respectively. The in vivo clearance (CL) is 11.0, 11.8 and 3.5 mL/min/kg in mouse, rat and dog, respectively, revealing a low extraction ratio. The volume of distribution in steady state (Vss) is 0.4, 1.4 and 1.4 L/kg in those species, respectively, indicating a moderate to low distribution pattern. Volitinib also displays linear pharmacokinetics (PK) in the dose ranges of 1 to 25 mg/kg in rat and 2 to 10 mg/kg in dog. Food hardly affects its PK profile in dog. In contrast, volitinib in monkey shows a notably high extraction ratio (CL=17.2 mL/min/kg) consistent with the in vitro metabolism result. Considering the rapid absorption of volitinib (Tmax=1.9 h) and moderately low distribution (Vss=0.7 L/kg), the poor oral bioavailability (1.9%) of volitinib in monkey is considered to be the result of excessive first-pass extraction. Overall, volitinib exhibits favorable preclinical PK/ADME properties[3].


Cell Research:


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  • Cell lines: NCI-H441 cells
  • Concentrations: --
  • Incubation Time: 1 h
  • Method:

    NCI-H441 cells are plated at a density of 15,000 cells/well in RPMI-1640 medium with 10% FBS in 96-well plates. After incubation overnight, cells are then treated with serially diluted test compounds at 37 ℃ for 1 h. Then the medium is removed, and cells are lysed in 100 μL/well lysis buffer (1% NP-40, 20 mM Tris/pH 8.0, 137 mM NaCl, 10% glycerol, 2 mM EDTA, 1 mM activated sodium orthovanadate, 10 mg/mL Aprotinin, 10 mg/mL Leupeptin). The plates containing cell lysate are kept at -80℃ overnight. The next day, the plates are thawed on ice, mixed gently. 25 μL/well of lysates are added into the assay plates pre-coated with anti-p-Met antibody to detect p-c-Met signal. p-c-Met level is determined at 450 nm and 570 nm.

    (Only for Reference)
Animal Research:


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  • Animal Models: Female athymic mice
  • Dosages: 1.0, 2.5 and 10.0 mg/kg (oral); 2.5 mg/kg (i.v.)
  • Administration: oral administration/i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 16 mg/mL (46.32 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 345.36


CAS No. 1313725-88-0
Storage powder
in solvent
Synonyms HMPL-504, Volitinib
Smiles CC(C1=CN2C=CN=C2C=C1)N3C4=NC(=CN=C4N=N3)C5=CN(N=C5)C

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT04187456 Completed Drug: Savolitinib|Drug: Midazolam Solid Tumors AstraZeneca|Parexel December 27 2019 Phase 1
NCT04179071 Completed Drug: Savolitinib|Drug: Famotidine Solid Tumours AstraZeneca|Parexel December 13 2019 Phase 1
NCT04121910 Completed Drug: Savolitinib|Drug: Itraconazole Solid Tumour AstraZeneca|Parexel November 7 2019 Phase 1
NCT04118842 Completed Drug: Savolitinib|Drug: Rifampicin Solid Tumors AstraZeneca October 17 2019 Phase 1

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c-Met Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID