For research use only.

Catalog No.S7038 Synonyms: BU-4061T,Aids010837

10 publications

Epoxomicin Chemical Structure

Molecular Weight(MW): 554.72

Epoxomicin is a selective proteasome inhibitor with anti-inflammatory activity, inhibits primarily the CH-L activity of the 20S proteasome, while T-L and PGPH catalytic activities are also inhibited at 100- and 1000-fold reduced rate. Epoxomicin promotes apoptosis.

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Selleck's Epoxomicin has been cited by 10 publications

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  • Epoxomicin did not inhibit the EV71-induced PMLIII and IV degradation. Cell lysates were prepared from 293T cells infected or mock-infected with EV71 at a multiplicity of infection (MOI) of 5 for 24 or 48 h in the presence or absence of epoxomicin (1 µM). At 24 h post-infection (p.i.) (left panels) or 48 h p.i. (right panels), 60 μg of protein extracted from each treatment were separated on SDS-PAGE and analyzed by performing a Western blot analysis using antibodies specific for PMLIII and IV (top panels), VP1 (middle panels), and GAPDH, which served as an internal loading control (bottom panels). PMLIII and IV were quantified by performing densitometry and are presented relative to the mock infection, which was set as 1.0. VP1 was quantified and is presented relative to that in the EV71-infected 293T cells at 24 h p.i. (left panels) or 48 h p.i. (right panels). The density value of VP1 without epoxomicin is set as 1.0. The experiment was performed three times, and representative results are shown.

    Front Immunol, 2018, 9:1268. Epoxomicin purchased from Selleck.

    Immature monocyte-derived dendritic cells (moDCs) were incubated 30 min prior and during the 3 h antigen (gp100/AZN-D1) pulse with 0.1% DMSO (vehicle), chloroquine (25 µM), MG132 (10 µM), epoxomicin (0.25 µM), and cathepsin S inhibitor (5 µM). Groups are significantly different compared to AZN-D1.

    Front Immunol, 2018, 9:1231. Epoxomicin purchased from Selleck.

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Biological Activity

Description Epoxomicin is a selective proteasome inhibitor with anti-inflammatory activity, inhibits primarily the CH-L activity of the 20S proteasome, while T-L and PGPH catalytic activities are also inhibited at 100- and 1000-fold reduced rate. Epoxomicin promotes apoptosis.
Features Epoxomicin is a natural product isolated from an Actinomycetes species.
20S proteasome [1]
In vitro

Epoxomicin covalently binds to the LMP7, X, MECL1, and Z catalytic subunits of the proteasome. Epoxomicin (100 nM) results in a 30-fold increase in the levels of p53 protein, a known target of the proteasome, in HUVECs. Epoxomicin (10 μM) results in the accumulation of ubiquitinated proteins in HeLa cells. Epoxomicin (10 μM) inhibits IκBα degradation by 10-fold in HeLa cells. Epoxomicin (10 μM) produces a significant dose-dependent reduction in TNF-α-stimulated NF-κB DNA-binding activity in HeLa cells. [1] Epoxomicin inhibits proliferating of EL4 lymphoma cells with biotinylated chimerae with IC50 of 4 nM. [2] Epoxomicin (1 μM) leads to a reduction of LCMV GP33 presentation and an enhancement of GP276 presentation. [3] Epoxomicin inhibits growth of Babesia bigemina with IC50 of 4 nM. Epoxomicin (0.5 mg/kg and 0.05 mg/kg) results in peak parasitemia levels of 34.8% and 42.3% in B. microti. [4] Epoxomicin (100 nM) decreases the total parasitemia by 78%, 86% and 77% in Plasmodium falciparum. Epoxomicin (10 μM) inhibits gametogenesis and exflagellation as well as development into oocysts of anopheles mosquitoes. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC3 cell NIHDcI5Rem:uaX\ldoF1cW:wIHHzd4F6 M1PBUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGxibHnu[UwhUUN3ME2wMlAxOSEQvF2= NXvZfWV5OTZ4OE[1N|c>
WM266.4 cells NXfUW|ZxS3m2b4TvfIlkcXS7IHHzd4F6 NFHvd3A4OiCq NVW3R2tiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hX01{Nk[uOEBk\WyuczDh[pRmeiB5MjDodpMh[nliQWTQcIl1\SCjc4PhfUwhUUN3ME2wMlAxPDhizszN NEO0[YkzOjJyNki2PS=>
human LCL cells M2G2emZ2dmO2aX;uJIF{e2G7 NY[2NItmOTJiaB?= MUPJcohq[mm2aX;uJI9nKGOqeX3veJJ6eHOrbj3sbYtmKGGldHn2bZR6KG:oIEKwV{Bxem:2ZXHzc41mKGKndHGyJIlvKGi3bXHuJGxEVCClZXzsd{Bi\nSncjCxNkBpenNuIFnDOVA:OC5yMEWg{txO NV7Nc21vOjB4OEe2NFk>
human DLD1 cells MXLGeY5kfGmxbjDhd5NigQ>? NWnLU|UzUW6qaXLpeIlwdiCxZjDjbJlud3S{eYDzbY4udGmtZTDhZ5Rqfmm2eTDv[kAzOFNicILveIVie2:vZTDpckBpfW2jbjDEUGQyKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigOHVjNUy3YzDn[Y5mKHW|aX7nJHN2[2OrbonsMWxmfS2OZYWtWoFtNVS7cj3BUWMh[XNic4Xid5Rz[XSnIHHmeIVzKDZiaILzJIJ6KHOyZXP0do9ndHWxcn;t[ZRzcWNiYX7hcJl{cXNuIFnDOVA:OC5yME[g{txO NHi4b2UzOjJyNki2PS=>
HEK293 cells M2K0PGZ2dmO2aX;uJIF{e2G7 MkfyNkBp M1zvSmlvcGmkaYTpc44hd2ZiY3j5cY91enmyc3nuMYxqc2ViYXP0bZZqfHlib3[gdJJwfGWjc3;t[UBj\XSjLUWgd5VjfW6rdDDpckBJTUt{OUOgZ4VtdHNidYPpcochW3WlLVzMWnkuT2yxIHHzJJN2[nO2cnH0[UBqdmO3YnH0[YQh\m:{IEKgbJJ{KHC{aX;yJJRwKHO3YoP0doF1\SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAyOCCvaX7zJIJ6KGOnbHytZoF{\WRicILveIVie2:vZT3HcI8h[mW2YUWgZZN{[XluIFnDOVA:OC5yMk[g{txO MlW2NlM2PDB5OUC=
lymphoblastoid cells MlPZSpVv[3Srb36gZZN{[Xl? M3mwVmlvcGmkaYTpc44hd2ZiY3HzdIF{\SCuaXvlJFIxWyCycn;0[YF{d22nIHHjeIl3cXS7IHnuJIx6dXCqb3LsZZN1d2mmIHPlcIx{NCCLQ{WwQVQvPTZizszN NVKwZlM{OTd7OU[5PFc>

... Click to View More Cell Line Experimental Data

In vivo Epoxomicin (0.58 mg/kg per day) reduces the CS response by 44% relative to the control group of mice treated with vehicle alone. Epoxomicin (2.9 mg/kg) potently inhibits the irritant-associated inflammatory response by 95% when ear edema measurements are made 24 hours postchallenge in mice. [1]


Animal Research:[1]
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  • Animal Models: BALB/c mice
  • Dosages: 2.9 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (180.27 mM)
Water Insoluble
Ethanol '100 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 554.72


CAS No. 134381-21-8
Storage powder
in solvent
Synonyms BU-4061T,Aids010837
Smiles CCC(C)C(NC(=O)C(C(C)CC)N(C)C(C)=O)C(=O)NC(=O)C(NC(CC(C)C)C(=O)C1(C)CO1)C(C)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID