For research use only.

Catalog No.S7038 Synonyms: BU-4061T,Aids010837

10 publications

Epoxomicin Chemical Structure

CAS No. 134381-21-8

Epoxomicin (BU-4061T, Aids010837) is a selective proteasome inhibitor with anti-inflammatory activity, inhibits primarily the CH-L activity of the 20S proteasome, while T-L and PGPH catalytic activities are also inhibited at 100- and 1000-fold reduced rate. Epoxomicin promotes apoptosis.

Size Price Stock Quantity  
EUR 183 In stock
EUR 567 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Epoxomicin has been cited by 10 publications

2 Customer Reviews

  • Epoxomicin did not inhibit the EV71-induced PMLIII and IV degradation. Cell lysates were prepared from 293T cells infected or mock-infected with EV71 at a multiplicity of infection (MOI) of 5 for 24 or 48 h in the presence or absence of epoxomicin (1 µM). At 24 h post-infection (p.i.) (left panels) or 48 h p.i. (right panels), 60 μg of protein extracted from each treatment were separated on SDS-PAGE and analyzed by performing a Western blot analysis using antibodies specific for PMLIII and IV (top panels), VP1 (middle panels), and GAPDH, which served as an internal loading control (bottom panels). PMLIII and IV were quantified by performing densitometry and are presented relative to the mock infection, which was set as 1.0. VP1 was quantified and is presented relative to that in the EV71-infected 293T cells at 24 h p.i. (left panels) or 48 h p.i. (right panels). The density value of VP1 without epoxomicin is set as 1.0. The experiment was performed three times, and representative results are shown.

    Front Immunol, 2018, 9:1268. Epoxomicin purchased from Selleck.

    Immature monocyte-derived dendritic cells (moDCs) were incubated 30 min prior and during the 3 h antigen (gp100/AZN-D1) pulse with 0.1% DMSO (vehicle), chloroquine (25 µM), MG132 (10 µM), epoxomicin (0.25 µM), and cathepsin S inhibitor (5 µM). Groups are significantly different compared to AZN-D1.

    Front Immunol, 2018, 9:1231. Epoxomicin purchased from Selleck.

Purity & Quality Control

Choose Selective Proteasome Inhibitors

Biological Activity

Description Epoxomicin (BU-4061T, Aids010837) is a selective proteasome inhibitor with anti-inflammatory activity, inhibits primarily the CH-L activity of the 20S proteasome, while T-L and PGPH catalytic activities are also inhibited at 100- and 1000-fold reduced rate. Epoxomicin promotes apoptosis.
Features Epoxomicin is a natural product isolated from an Actinomycetes species.
20S proteasome [1]
In vitro

Epoxomicin covalently binds to the LMP7, X, MECL1, and Z catalytic subunits of the proteasome. Epoxomicin (100 nM) results in a 30-fold increase in the levels of p53 protein, a known target of the proteasome, in HUVECs. Epoxomicin (10 μM) results in the accumulation of ubiquitinated proteins in HeLa cells. Epoxomicin (10 μM) inhibits IκBα degradation by 10-fold in HeLa cells. Epoxomicin (10 μM) produces a significant dose-dependent reduction in TNF-α-stimulated NF-κB DNA-binding activity in HeLa cells. [1] Epoxomicin inhibits proliferating of EL4 lymphoma cells with biotinylated chimerae with IC50 of 4 nM. [2] Epoxomicin (1 μM) leads to a reduction of LCMV GP33 presentation and an enhancement of GP276 presentation. [3] Epoxomicin inhibits growth of Babesia bigemina with IC50 of 4 nM. Epoxomicin (0.5 mg/kg and 0.05 mg/kg) results in peak parasitemia levels of 34.8% and 42.3% in B. microti. [4] Epoxomicin (100 nM) decreases the total parasitemia by 78%, 86% and 77% in Plasmodium falciparum. Epoxomicin (10 μM) inhibits gametogenesis and exflagellation as well as development into oocysts of anopheles mosquitoes. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC3 cell M{jRbHBzd2yrZnXyZZRqd25iYYPzZZk> MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxtKGyrbnWsJGlEPTB;MD6wNFEh|ryP M2\vVVE3Pjh4NUO3
WM266.4 cells MY\DfZRwfG:6aXPpeJkh[XO|YYm= NHHie2s4OiCq M4fiXWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHdOOjZ4LkSgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEGWUHzpeIUh[XO|YYmsJGlEPTB;MD6wNFQ5KM7:TR?= NF3iXlQzOjJyNki2PS=>
human LCL cells M335ZmZ2dmO2aX;uJIF{e2G7 MkDmNVIhcA>? NH31NXdKdmirYnn0bY9vKG:oIHPofY1wfHK7cIPpck1tcWunIHHjeIl3cXS7IH;mJFIxWyCycn;0[YF{d22nIHLleIEzKGmwIHj1cYFvKEyFTDDj[YxteyCjZoTldkAyOiCqcoOsJGlEPTB;MD6wNFUh|ryP M3y5flIxPjh5NkC5
human DLD1 cells M1m4NGZ2dmO2aX;uJIF{e2G7 NYm2blE3UW6qaXLpeIlwdiCxZjDjbJlud3S{eYDzbY4udGmtZTDhZ5Rqfmm2eTDv[kAzOFNicILveIVie2:vZTDpckBpfW2jbjDEUGQyKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigOHVjNUy3YzDn[Y5mKHW|aX7nJHN2[2OrbonsMWxmfS2OZYWtWoFtNVS7cj3BUWMh[XNic4Xid5Rz[XSnIHHmeIVzKDZiaILzJIJ6KHOyZXP0do9ndHWxcn;t[ZRzcWNiYX7hcJl{cXNuIFnDOVA:OC5yME[g{txO NV\0SFJ7OjJ{ME[4Olk>
HEK293 cells MmXoSpVv[3Srb36gZZN{[Xl? NFG3fmIzKGh? MVnJcohq[mm2aX;uJI9nKGOqeX3veJJ6eHOrbj3sbYtmKGGldHn2bZR6KG:oIIDyc5Rm[XOxbXWgZoV1[S13IIP1ZpVvcXRiaX6gTGVMOjl|IHPlcIx{KHW|aX7nJHN2[y2OTG\ZMWdtdyCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB{IHjyd{BxemmxcjD0c{B{fWK|dILheIUh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNVAhdWmwczDifUBk\WyuLXLhd4VlKHC{b4TlZZNwdWVvR3zvJIJmfGF3IHHzd4F6NCCLQ{WwQVAvODJ4IN88US=> MVmyN|U1ODd7MB?=
lymphoblastoid cells Mn;DSpVv[3Srb36gZZN{[Xl? MoGyTY5pcWKrdHnvckBw\iClYYPwZZNmKGyra3WgNlBUKHC{b4TlZZNwdWViYXP0bZZqfHliaX6gcJlueGixYnzhd5RwcWRiY3XscJMtKEmFNUC9OE42PiEQvF2= M4nSV|E4QTl4OUi3

... Click to View More Cell Line Experimental Data

In vivo Epoxomicin (0.58 mg/kg per day) reduces the CS response by 44% relative to the control group of mice treated with vehicle alone. Epoxomicin (2.9 mg/kg) potently inhibits the irritant-associated inflammatory response by 95% when ear edema measurements are made 24 hours postchallenge in mice. [1]


Animal Research:[1]
- Collapse
  • Animal Models: BALB/c mice
  • Dosages: 2.9 mg/kg
  • Administration: intraperitoneal injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (180.27 mM)
Water Insoluble
Ethanol ''100 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 554.72


CAS No. 134381-21-8
Storage powder
in solvent
Synonyms BU-4061T,Aids010837
Smiles CCC(C)C(C(=O)NC(C(C)O)C(=O)NC(CC(C)C)C(=O)C1(CO1)C)NC(=O)C(C(C)CC)N(C)C(=O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Proteasome Signaling Pathway Map

Related Proteasome Products

Tags: buy Epoxomicin | Epoxomicin supplier | purchase Epoxomicin | Epoxomicin cost | Epoxomicin manufacturer | order Epoxomicin | Epoxomicin distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID