ONX-0914 (PR-957)

For research use only.

Catalog No.S7172

13 publications

ONX-0914 (PR-957) Chemical Structure

CAS No. 960374-59-8

ONX-0914 (PR-957) is a potent and selective immunoproteasome inhibitor with minimal cross-reactivity for the constitutive proteasome in a cell-free assay.

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Selleck's ONX-0914 (PR-957) has been cited by 13 publications

2 Customer Reviews

  • Dependence of SPRI signal on receptor concentration: (a) ONX 0914 inhibitor, (b) inhibitor 5. 20Si concentration: 2.8 μg/mL, pH 7.4

    Cell Mol Bioeng, 2017, 10(2):174-185. ONX-0914 (PR-957) purchased from Selleck.

  • Mice were treated with daily doses of 10 mg/kg ONX-0914 iv or vehicle (n = 5–6). Not significant by log-rank test.

    Parasite Immunol, 2015, 37(11):599-604.. ONX-0914 (PR-957) purchased from Selleck.

Purity & Quality Control

Choose Selective Proteasome Inhibitors

Biological Activity

Description ONX-0914 (PR-957) is a potent and selective immunoproteasome inhibitor with minimal cross-reactivity for the constitutive proteasome in a cell-free assay.
Features The first highly selective, small molecule inhibitor of the immunoproteasome. Potential use in cancer and autoimmune diseases (e.g. rheumatoid arthritis, inflammatory bowel disease, and lupus).
LMP7 [1]
(Cell-free assay)
~10 nM
In vitro

ONX-0914 is 20- to 40-fold more selective for LMP7 over the next most sensitive sites, β5 or LMP2. ONX-0914 blocks presentation of LMP7-specific, MHC-I–restricted antigens in vitro and in vivo with minimal cross-reactivity for the constitutive proteasome. Selective inhibition of LMP7 by ONX-0914 blocks production of interleukin-23 (IL-23) by activated monocytes and interferon-gamma and IL-2 by T cells. LMP7 inhibition blocks production of IL-23 by ~90% and of tumor necrosis factor-α (TNF-α) and IL-6 by ~50%.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human Raji cells NUW0cnp1TnWwY4Tpc44h[XO|YYm= NIjySmNKdmirYnn0bY9vKG:oIIDyc5Rm[XOxbXWgd5VjfW6rdDDi[ZRiNTWrIHnuJIh2dWGwIGLhbokh[2WubIOgeZNqdmdiQl;ETXB[NU6FMEC1JIJ6KG[udX;y[ZNk\W62IHTlcpNqfG:vZYTyfUwhUUN3ME21Mlchdk1? NWrrclFHOjVyME[3OFY>
human RPMI8226 cells MUTGeY5kfGmxbjDhd5NigQ>? MoPPTY5pcWKrdHnvckBw\iCycn;0[YF{d22nIIP1ZpVvcXRiYnX0ZU02cSCrbjDoeY1idiCUUF3JPFIzPiClZXzsd{B2e2mwZzDCU2RKWFlvTlOwNFUh[nliZnz1c5Jme2OnboSg[IVve2m2b33leJJ6NCCLQ{WwQVAvODF6IN88US=> MXqyOVAxPjd2Nh?=
human Raji cells M37OfWZ2dmO2aX;uJIF{e2G7 M2O4Z2lvcGmkaYTpc44hd2ZiMkDzJJBzd3SnYYPvcYUhe3WkdX7peEBj\XSjLUXjJIlvKGi3bXHuJHJicmliY3XscJMhfXOrbnegRm9FUVC\LV7DNFA2KGK7IH\seY9z\XOlZX70JIRmdnOrdH;t[ZRzgSxiSVO1NF0xNjB3NDFOwG0> NVLzSYl4OjVyME[3OFY>
human RPMI8226 cells MXvGeY5kfGmxbjDhd5NigQ>? NXzCOYpwUW6qaXLpeIlwdiCxZjCyNJMheHKxdHXhd49u\SC|dXL1col1KGKndHGtOYMhcW5iaIXtZY4hWlCPSUiyNlYh[2WubIOgeZNqdmdiQl;ETXB[NU6FMEC1JIJ6KG[udX;y[ZNk\W62IHTlcpNqfG:vZYTyfUwhUUN3ME2wMlE5KM7:TR?= NXPxNmdLOjVyME[3OFY>
human RPMI8226 cells MX3GeY5kfGmxbjDhd5NigQ>? NWX6fWpZUW6qaXLpeIlwdiCxZjDwdo91\WG|b33lJJN2[nWwaYSgZoV1[S1zaTDpckBpfW2jbjDSVG1KQDJ{NjDj[YxteyC3c3nu[{BDV0SLUGmtUmMxODFiYomg[ox2d3Knc3PlcpQh\GWwc3n0c41mfHK7LDDJR|UxRTBwM{Sg{txO NEHzW4UzPTByNke0Oi=>
human Raji cells MoHHSpVv[3Srb36gZZN{[Xl? MWXJcohq[mm2aX;uJI9nKHC{b4TlZZNwdWVic4XieY5qfCCkZYThMVFqKGmwIHj1cYFvKFKjanmgZ4VtdHNidYPpcochSk:GSWDZMUBmeG:6b33pZ4lvKGK7IH\seY9z\XOlZX70JIRmdnOrdH;t[ZRzgSxiSVO1NF0xNjR4IN88US=> M{DZ[FI2ODB4N{S2
human Raji cells MV3GeY5kfGmxbjDhd5NigQ>? NEf1NGxKdmirYnn0bY9vKG:oIIDyc5Rm[XOxbXWgd5VjfW6rdDDi[ZRiNTKrIHnuJIh2dWGwIGLhbokh[2WubIOgeZNqdmdiQl;ETXB[NSCncH;4c41q[2mwIHL5JIZtfW:{ZYPj[Y51KGSnboPpeI9u\XS{eTygTWM2OD1yLkW5JO69VQ>? M1;I[VI2ODB4N{S2
human RPMI8226 cells NWrJVHhFTnWwY4Tpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKHC{b4TlZZNwdWVic4XieY5qfCCkZYThMVJqKGmwIHj1cYFvKFKSTVm4NlI3KGOnbHzzJJV{cW6pIFLPSGlRYS2ncH;4c41q[2mwIHL5JIZtfW:{ZYPj[Y51KGSnboPpeI9u\XS{eTygTWM2OD1yLkW5JO69VQ>? M4Dyc|I2ODB4N{S2
human Raji cells MWLGeY5kfGmxbjDhd5NigQ>? NF73cFVKdmirYnn0bY9vKG:oIEKwd{Bxem:2ZXHzc41mKHO3YoXubZQh[mW2YT2yZ{BqdiCqdX3hckBT[WqrIHPlcIx{KHW|aX7nJGJQTEmSWT2g[ZBwgG:vaXPpckBjgSCobIXvdoV{[2WwdDDk[Y5{cXSxbXX0dpktKEmFNUC9NU4yKM7:TR?= MUWyOVAxPjd2Nh?=
human RPMI8226 cells NX:2[mxPTnWwY4Tpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKDJyczDwdo91\WG|b33lJJN2[nWwaYSgZoV1[S1{YzDpckBpfW2jbjDSVG1KQDJ{NjDj[YxteyC3c3nu[{BDV0SLUGmt[ZBwgG:vaXPpckBjgSCobIXvdoV{[2WwdDDk[Y5{cXSxbXX0dpk> NWLFZWpTOjVyME[3OFY>

... Click to View More Cell Line Experimental Data

In vivo In mouse models of rheumatoid arthritis and lupus, ONX-0914 treatment reverses signs of disease and results in reductions in cellular infiltration, cytokine production and autoantibody levels at well-tolerated doses. The maximum tolerated dose (MTD) of ONX-0914 in mice to be 30 mg/kg body weight. IFN-g release is inhibited by ~60% at LMP7-selective concentrations of ONX-0914 and by ~90% at higher concentrations. Production of IL-2 is also inhibited by ~50%.[1]


Animal Research:[1]
- Collapse
  • Animal Models: collagen antibody–induced arthritis (CAIA) and collagen-induced arthritis (CIA)
  • Dosages: 2, 6 or 10 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (172.21 mM)
Ethanol 100 mg/mL (172.21 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+castor oil
For best results, use promptly after mixing.
10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 580.67


CAS No. 960374-59-8
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID