For research use only.

Catalog No.S7377

2 publications

CAS No. 9087-70-1

Aprotinin is a small protein serine protease inhibitor (Kd=0.06 pM for bovine β-trypsin), used to reduce perioperative blood loss and transfusion.

Selleck's Aprotinin has been cited by 2 publications

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Biological Activity

Description Aprotinin is a small protein serine protease inhibitor (Kd=0.06 pM for bovine β-trypsin), used to reduce perioperative blood loss and transfusion.
Thrombin [1]
(Cell-free assay)
Trypsin [1]
(Cell-free assay)
kallikrein [1]
(Cell-free assay)
chymotrypsin [1]
(Cell-free assay)
trypsinogen [1]
(Cell-free assay)
0.06 pM(Kd) 0.8 nM(Kd) 9.5 nM(Kd) 2 μM(Kd)
In vitro

Aprotinin is an antifibrinolytic molecule that inhibits trypsin and related proteolytic enzymes. In cell biology, aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues. In the presence of aprotinin, the fibrinolytic activity is inhibited concentration dependently and the coagulation time is prolonged. Aprotinin is an effective inhibitor of the contact (intrinsic) coagulation pathway in vitro[2].

In vivo Aprotinin inhibits clot lysis in vitro as well as rat-tail bleeding time in vivo and prolongs coagulation time in human plasma. In a rat arteriovenous shunt model, aprotinin reduces thrombus weight [2].


Cell Research:


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  • Cell lines: mouse G8-1 and C2C12 skeletal muscle myoblasts
  • Concentrations: 2 TIU/ml
  • Incubation Time: 12hrs
  • Method:

    Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days.

    (Only for Reference)
Animal Research:


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  • Animal Models: Male Wistar rats
  • Dosages: 1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h
  • Administration: bolus injection followed by a maintenance infusion(i.p.)
    (Only for Reference)

Solubility (25°C)

In vitro Water 100 mg/mL (15.35 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 6511.51


CAS No. 9087-70-1
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01554956 Completed Biological: Human Plasminogen Ligneous Conjunctivitis Kedrion S.p.A. May 22 2013 Phase 2|Phase 3
NCT00481806 Unknown status -- Coronary Artery Bypass|Thrombosis University of Arizona|University of Maryland Baltimore County February 2007 --
NCT00354900 Terminated Drug: Aprotinin Metastatic Breast Cancer Dartmouth-Hitchcock Medical Center July 2006 Phase 1
NCT00131040 Completed Drug: Aprotinin Ischemic Heart Disease|Angina Pectoris Imperial College London|British Heart Foundation January 2003 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID