Leupeptin Hemisulfate

Catalog No.S7380

For research use only.

Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.

Leupeptin Hemisulfate Chemical Structure

CAS No. 103476-89-7

Selleck's Leupeptin Hemisulfate has been cited by 22 publications

Purity & Quality Control

Choose Selective Serine Protease Inhibitors

Biological Activity

Description Leupeptin Hemisulfate is a reversible inhibitor of serine and cysteine proteases. It inhibits cathepsin B (Ki = 6 nM), calpain (Ki = 10 nM), trypsin (Ki = 35 nM), plasmin (Ki = 3.4 μM), and kallikrein (Ki = 19 μM), and has no effect against chymotrypsin, elastase, renin, or pepsin.
Targets
Cysteine protease [1] serine protease [1]
In vitro

Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. [1] Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. [2] Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 MV3GeY5kfGmxbjDhd5NigQ>? NEDkPYEzOCCvaX7z NEm4fVZKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IH3heJJqeHSjc3WgZ4F1[Wy7dHnjJIRwdWGrbjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHmeIVzKDJyIH3pcpMtKEurIE2gNU46KM7:TT6= NEDXUIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[4OFU6Pyd-MkC2PFQ2QTd:L3G+
HEK293 MX3GeY5kfGmxbjDhd5NigQ>? MmXJNlAhdWmwcx?= NI\VVIdKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IH3heJJqeHSjc3WgNkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIlvKGOxbnTpeIlwdmWmIH3l[Il2dSCjZoTldkAzOCCvaX7zMEBMcSB;IEKuOEDPxE1w MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ6NEW5O{c,OjB4OES1PVc9N2F-
HEK293 NE[1ZmFHfW6ldHnvckBie3OjeR?= M2THUlIxKG2rboO= M163[mlvcGmkaYTpc44hd2ZiaIXtZY4heHW{aX\p[YQhdWG2cnnweIF{\SB{IHPheIFtgXSrYzDkc41icW5iZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAzOCCvaX7zMEBMcSB;IESuNUDPxE1w NIHyPHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[4OFU6Pyd-MkC2PFQ2QTd:L3G+

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 95 mg/mL warmed
(199.75 mM)
Water 95 mg/mL
(199.75 mM)
Ethanol '95 mg/mL

Chemical Information

Molecular Weight 475.59
Formula

C20H38N6O4.1/2H2SO4

CAS No. 103476-89-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCCN=C(N)N)C=O)NC(=O)C.CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCCN=C(N)N)C=O)NC(=O)C.OS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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