Adenosine Dialdehyde (ADOX)

Catalog No.S8608

Adenosine Dialdehyde (ADOX) Chemical Structure

Molecular Weight(MW): 265.23

Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.

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Biological Activity

Description Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.
Targets
AdoHcy hydrolase [1]
(Cell-free)
40 nM
In vitro

Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes[4].

Assay
Methods Test Index PMID
Western blot
p-Tau / Tau ; 

PubMed: 23943618     


Alterations in one-carbon metabolism affect the distribution of endogenous PP2A and Tau at the plasma membrane. A, Western blot analysis of plasma membrane fractions (30 μg of proteins) purified from N2a cells incubated for 2 h with 50 μM adenosine dialdehyde (AdOx), 10 mM cycloleucine (CL), or vehicle alone (Control) in normal RPMI medium containing 1% dialyzed serum. B, the loss of LCMT1, methylated C, and Tau dephosphorylated at the Tau-1 epitope in the plasma membrane was associated with an increase of Tau phosphorylated at Ser-422 in cytosolic fractions purified from the same cells. pSer422, phospho-Ser-422. In Aand B, representative blots from three separate experiments are shown.

AUF1; 

PubMed: 22379108     


Cells (RAW-264.7) were treated with AdOx (5 µM) for 48 h, and lysates were immunoblotted for AUF1 isoforms. Loading control was α-tubulin.

23943618 22379108
In vivo AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death[3].

Protocol

Cell Research:

[1]

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  • Cell lines: Hela cells
  • Concentrations: 0, 10, 20 or 40 μM
  • Incubation Time: 24 and 48 h
  • Method:

    Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).


    (Only for Reference)
Animal Research:

[3]

+ Expand
  • Animal Models: Murine Neuroblastoma Tumor Model (adult male A/J mice)
  • Formulation: DMSO (3.3%, v/v), ethanol (50%, v/v) and saline (46.7%, v/v)
  • Dosages: 1.5 to 2.5 mg/kg/day
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 19 mg/mL (71.63 mM)
Water 3 mg/mL (11.31 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 265.23
Formula

C10H11N5O4

CAS No. 34240-05-6
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Transferase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID