Adenosine Dialdehyde (ADOX)

Catalog No.S8608

For research use only.

Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.

Adenosine Dialdehyde (ADOX) Chemical Structure

CAS No. 34240-05-6

Selleck's Adenosine Dialdehyde (ADOX) has been cited by 4 Publications

Purity & Quality Control

Choose Selective Transferase Inhibitors

Biological Activity

Description Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.
Targets
AdoHcy hydrolase [1]
(Cell-free)
40 nM
In vitro

Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes[4].

Assay
Methods Test Index PMID
Western blot p-Tau / Tau ; AUF1 23943618 22379108
In vivo AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death[3].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Hela cells
  • Concentrations: 0, 10, 20 or 40 μM
  • Incubation Time: 24 and 48 h
  • Method:

    Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: Murine Neuroblastoma Tumor Model (adult male A/J mice)
  • Dosages: 1.5 to 2.5 mg/kg/day
  • Administration: s.c.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 19 mg/mL
(71.63 mM)
Water 3 mg/mL
(11.31 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 265.23
Formula

C10H11N5O4

CAS No. 34240-05-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=NC(=C2C(=N1)N(C=N2)C(C=O)OC(CO)C=O)N

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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