Adenosine Dialdehyde (ADOX)

Catalog No.S8608

For research use only.

Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.

Adenosine Dialdehyde (ADOX) Chemical Structure

CAS No. 34240-05-6

Selleck's Adenosine Dialdehyde (ADOX) has been cited by 5 Publications

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Biological Activity

Description Adenosine Dialdehyde (ADOX) is an adenosine analog and S-adenosylmethionine-dependent methyltransferase inhibitor with an IC50 of 40 nM.
AdoHcy hydrolase [1]
40 nM
In vitro

Adenosine dialdehyde (AdOx), an indirect inhibitor that can be incorporated by cells. AdOx inhibits S-adenosyl-L-homocystein hydrolase, resulting in the accumulation of S-adenosyl-L-homo cystein (Adoicy), a product inhibitor of methyltransferases that utilize S-adenosyl-L-methionine (AdoMet) as the methyl group donor[2]. AdOx inhibited the Tax-activated NF-κB pathway, resulting in reactivation of p53 and induction of p53 target genes. Analysis of the NF-κB pathway demonstrated that AdOx treatment resulted in degradation of the IκB kinase complex and inhibition of NF-κB through stabilization of the NF-κB inhibitor IκBα. AdOx induced G2/M cell cycle arrest and cell death in HTLV-1-transformed but not control lymphocytes[4].

Methods Test Index PMID
Western blot p-Tau / Tau ; AUF1 23943618 22379108
In vivo AdOx exerts a potent inhibitory effect on the in situ growth of established murine neuroblastoma (MNB) tumors, prolongs the life span of tumor bearing mice, and does not suppress hematopoiesis when administered by steady state infusion. AdOx inhibits the replication of L1210 leukemia cells and increased life span approximately 40% when administered i.p. at a dose of 20 mg/kg/day until death[3].

Protocol (from reference)

Cell Research:


  • Cell lines: Hela cells
  • Concentrations: 0, 10, 20 or 40 μM
  • Incubation Time: 24 and 48 h
  • Method:

    Hela cells are grown in MEM medium supplemented with 10% fetal bovine serum at 37 ℃ in a 5% CO2 incubator. Treatment of cells with AdOx for various time periods was performed. The cells are harvested and washed with phosphate-buffered saline, resuspended in buffer A (phosphate-buffered saline with 5% glycerol, 1 mM sodium EGTA, 1 mM dithiothreitol, 0.5% Triton X-100 and Complete protease inhibitor cocktail).

Animal Research:


  • Animal Models: Murine Neuroblastoma Tumor Model (adult male A/J mice)
  • Dosages: 1.5 to 2.5 mg/kg/day
  • Administration: s.c.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 265.23


CAS No. 34240-05-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=NC(=C2C(=N1)N(C=N2)C(C=O)OC(CO)C=O)N

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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