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AOA (Aminooxyacetic acid) hemihydrochloride Transferase inhibitor

Name: AOA (Aminooxyacetic acid) hemihydrochloride
Brand: Selleck Chemicals
SKU: S4989
Availability: InStock
Rating: 5 (16 reviews)

Cat.No.S4989

AOA (Aminooxyacetic acid) hemihydrochloride is an inhibitor of aminobutyrate aminotransferase involved in amino acid and polyamine metabolism. AOA hemihydrochloride is also a CBS (cystathionine β-synthase) inhibitor. AOA hemihydrochloride inhibits the malate-aspartate shuttle (MAS).

Chemical Structure

Molecular Weight: 109.3

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Quality Control

Batch: Purity: 99%

Related Targets	Dehydrogenase HSP P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase
Other Transferase Inhibitors	FK866 (Daporinad) Tunicamycin Ezatiostat GGTI 298 Trifluoroacetate 2-Bromohexadecanoic acid (2-BP) OSMI-4 A-922500 (DGAT-1 Inhibitor 4a) PF-04620110 FIDAS-3 SHIN1(RZ-2994)

Solubility

In vitro
Batch:

DMSO : 22 mg/mL (201.28 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 22 mg/mL

Ethanol : 6 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.100mg/ml (10.06mM)	Taking the 1 mL working solution as an example, add 50 μL of 22 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.550mg/ml (5.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 11 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	109.3	Formula	C₂H₅NO₃._1/2HCl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	2921-14-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Carboxymethoxylamine hemihydrochloride, AOAA hemihydrochloride, Aminooxyacetate hemihydrochloride			SMILES	C(C(=O)O)ON.C(C(=O)O)ON.Cl

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	GABA-T Malate-aspartate shuttle
In vitro	Aminooxyacetic acid (AOAA) is a malate-aspartate shuttle inhibitor, leading to decreased intracellular ATP levels and altering cell cycle and increasing apoptosis and necrosis of C6 glioma cells, without affecting the survival of primary astrocyte cultures. AOAA also decreases the glycolytic rate and the levels of extracellular lactate and pyruvate, without affecting the mitochondrial membrane potential of C6 cells. Aminooxyacetic acid can selectively inhibit the proliferation of breast cancer cells.
In vivo	Aminooxyacetic acid suppresses the growth of breast adenocarcinoma in athymic mice. AOAA is much less toxic in rats than in mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/27157912/ [2] https://pubmed.ncbi.nlm.nih.gov/2809589/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-03-13)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06818175	RECRUITING	Low Back Pain; Chronic Low-back Pain; Acute Low Back Pain	Edward Via Virginia College of Osteopathic Medicine	2023-09-01
NCT06111248	COMPLETED	All Conditions Requiring Non-cardiac Surgery	Centre Hospitalier Universitaire de Nīmes	2024-02-05
NCT05402605	UNKNOWN	in Vitro Fertilization	Mỹ Đức Hospital	2022-08-29
NCT04519918	UNKNOWN	Fertilization	Tang-Du Hospital	2020-10-01
NCT03354013	COMPLETED	Embryo; Embryo Disorder; Genetic Disease	University Hospital, Ghent	2018-01-15
NCT04744753	COMPLETED	Infertility Primary	MOHAMED BEHERY	2017-07-15	PHASE4

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Handling Instructions

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